LOCUS       NZ_CP069889             1669 bp    DNA     linear   CON 17-JUN-2023
DEFINITION  Escherichia coli strain FDAARGOS_1289 plasmid unnamed7.
ACCESSION   NZ_CP069889
VERSION     NZ_CP069889.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN16357431
            Assembly: GCF_016903315.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1669)
  AUTHORS   Sokurenko,E., Tallon,L., Sadzewicz,L., Zhao,X., Boylan,J., Ott,S.,
            Bowen,H., Vavikolanu,K., Mehta,A., Aluvathingal,J., Nadendla,S.,
            Yan,Y. and Sichtig,H.
  TITLE     FDA dAtabase for Regulatory Grade micrObial Sequences (FDA-ARGOS):
            Supporting development and validation of Infectious Disease Dx tests
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1669)
  AUTHORS   Sokurenko,E., Tallon,L., Sadzewicz,L., Zhao,X., Boylan,J., Ott,S.,
            Bowen,H., Vavikolanu,K., Mehta,A., Aluvathingal,J., Nadendla,S.,
            Yan,Y. and Sichtig,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2021) Institute for Genome Sciences, University of
            Maryland School of Medicine, BioPark II, 801 W. Baltimore St., Suite
            619, Baltimore, MD 21201, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SMRT v. 8.0.0, HGAP v. 4
            Genome Coverage                   :: 1351.18x
            Sequencing Technology             :: Pacbio; Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 06/17/2023 05:17:14
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,308
            Genes (coding)                    :: 5,115
            CDSs (with protein)               :: 5,115
            Genes (RNA)                       :: 123
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 92
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 193
            CDSs (without protein)            :: 193
            Pseudo Genes (ambiguous residues) :: 0 of 193
            Pseudo Genes (frameshifted)       :: 64 of 193
            Pseudo Genes (incomplete)         :: 139 of 193
            Pseudo Genes (internal stop)      :: 44 of 193
            Pseudo Genes (multiple problems)  :: 46 of 193
            Pseudo Genes (short protein)      :: 4 of 193
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP069889.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..1669
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="FDAARGOS_1289"
                     /host="Homo sapiens"
                     /culture_collection="FDA:FDAARGOS_1289"
                     /db_xref="taxon:562"
                     /plasmid="unnamed7"
                     /geo_loc_name="USA: WA"
                     /collected_by="ARMADA"
     gene            complement(<1..1416)
                     /gene="csa"
                     /locus_tag="I6K19_RS26835"
                     /old_locus_tag="I6K19_26830"
     CDS             complement(<1..1416)
                     /gene="csa"
                     /locus_tag="I6K19_RS26835"
                     /old_locus_tag="I6K19_26830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_104355618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like pore-forming protein"
                     /protein_id="WP_204606484.1"
                     /translation="MAKELSGYGPTAGESMGGTGANLNQQGGNNNSNSGVHWGGGSGSG
                     NGGREHGSQTGWGWSKTNNPDVPPYVDDNGQVRITITNGLVKTPVYGVPGAGGNSDVQG
                     GYIPENPNDEVARKWDKNNLPREIDVSIDGFKYRVTLNDNGRAIGILRTGVRPYVGSEK
                     AKAGIMEKINHKTPEEIYEALGFNKDESQRQEKAKQQAEDAWDRLPPNVRKFDVDVEQF
                     HYLVVLDDYGNVLSVTRTAVRPYVGSEKAKAGIMDKVDHKTPEEIYEALGFNNEEPQRQ
                     NQAKKAAYDVFYSFSMNRDRIQSDVLNKTAEVISDIGNKVGDYLGDAYKSLAREIADDV
                     KNFQGKTIRSYDDAMASLNKVLSNPGFKFNRADSDALANVWRSIDAQDMANKLGNISKA
                     FKFADVVMKVEKVREKSIEGYETGNWGPLMLEVESWVLSGIASAVALGVFSATLGAYAL
                     SLGAPAIAVGIVGI"
                     /name="csa"
ORIGIN
        1 aatacctacg ataccgacag caatagcagg agccccaaga gataaggcat atgcccctaa
       61 tgttgctgaa aaaacaccca gggccacagc agaagcgatg ccactgagca cccaggactc
      121 cacctccagc atcagaggtc cccagttccc ggtttcatag ccctcgatgc ttttctcccg
      181 gaccttctca accttcatca ccacatcggc aaatttgaaa gccttgctga tattacccag
      241 tttatttgcc atatcctgag catcaatact tcgccagacg ttcgccagtg catcactgtc
      301 ggccctgtta aatttaaacc caggattaga caaaacttta tttaatgacg ccattgcatc
      361 atcatagcta cggatagttt tcccttggaa atttttgaca tcatcagcaa tttctcttgc
      421 taacgactta taagcatctc caagataatc accgacctta ttacctatat cagagataac
      481 ttcagcggtt ttattaagta catcactctg aatccggtca cggttcatac tgaaagaata
      541 aaacacatca taagcagctt ttttagcttg attctgtcgt tgaggctctt cattattaaa
      601 tcctaatgcc tcatatatct cttccggggt cttatggtcg actttatcca taataccagc
      661 cttagctttt tcactcccta cataaggacg aacagctgtt ctagttacag ataaaacatt
      721 accataatca tcaagcacca ctaaatagtg gaactgttca acatcaacat caaattttct
      781 cacattagga ggcaaacgat cccacgcatc ttcagcctgt tgttttgctt tttcctgccg
      841 ctgagattca tctttattaa aacctaacgc ctcatatatc tcctcaggag ttttatgatt
      901 gattttttcc ataatacccg ccttagcttt ttcactccct acgtaaggac ggacacctgt
      961 tcgaagaata ccgatagctc taccattatc attaagcgtt acacgatatt taaaaccatc
     1021 gatactcaca tcaatctctc gcggtaagtt gtttttatcc cacttacgag caacttcatc
     1081 attaggattt tcaggaatat agcccccctg aacatcacta tttccgccag ccccaggaac
     1141 tccatataca ggcgttttta ccagaccatt tgtgatggta atgcgcacct gcccattatc
     1201 atcaacataa ggcggcacat ccggattatt ggttttactc catccccacc ccgtctgact
     1261 tccatgttca cgacctccat taccagagcc tgaaccacca ccccagtgaa ctccactatt
     1321 actattatta tttccacctt gctgattcag attcgcccct gttcccccca tagactcacc
     1381 agcagttggg ccatatccac ttagttcttt agccataaat tcctctttga taattaaaac
     1441 aataaattaa aaacaatata ctgtacatat aaccactggt tttatgtaca gcaaaaccta
     1501 ctactcacca ttgtccatgt caagagcatg gattttcatt tttgcaataa ggatcacact
     1561 atggggaggc aggcattgag aacgtcgaaa cagaacaccg gagcaaatca ggatgagata
     1621 taaaactgtt ggatcatgaa aaaacggaga acgatgtgag caaatcacc
//
