LOCUS       NZ_CP069881             1596 bp    DNA     linear   CON 05-JAN-2024
DEFINITION  Klebsiella pneumoniae strain FDAARGOS_1323 plasmid unnamed8.
ACCESSION   NZ_CP069881
VERSION     NZ_CP069881.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN16357465
            Assembly: GCF_016903295.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 1596)
  AUTHORS   Sokurenko,E., Tallon,L., Sadzewicz,L., Zhao,X., Boylan,J., Ott,S.,
            Bowen,H., Vavikolanu,K., Mehta,A., Aluvathingal,J., Nadendla,S.,
            Yan,Y. and Sichtig,H.
  TITLE     FDA dAtabase for Regulatory Grade micrObial Sequences (FDA-ARGOS):
            Supporting development and validation of Infectious Disease Dx tests
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1596)
  AUTHORS   Sokurenko,E., Tallon,L., Sadzewicz,L., Zhao,X., Boylan,J., Ott,S.,
            Bowen,H., Vavikolanu,K., Mehta,A., Aluvathingal,J., Nadendla,S.,
            Yan,Y. and Sichtig,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2021) Institute for Genome Sciences, University of
            Maryland School of Medicine, BioPark II, 801 W. Baltimore St., Suite
            619, Baltimore, MD 21201, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SMRT v. 8.0.0, HGAP v. 4
            Genome Coverage                   :: 771.48x
            Sequencing Technology             :: Pacbio; Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/05/2024 07:40:22
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,312
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,080
            CDSs (with protein)               :: 5,080
            Genes (RNA)                       :: 124
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 13
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 48 of 108
            Pseudo Genes (incomplete)         :: 59 of 108
            Pseudo Genes (internal stop)      :: 15 of 108
            Pseudo Genes (multiple problems)  :: 14 of 108
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP069881.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..1596
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="FDAARGOS_1323"
                     /host="Homo sapiens"
                     /culture_collection="FDA:FDAARGOS_1323"
                     /db_xref="taxon:573"
                     /plasmid="unnamed8"
                     /geo_loc_name="USA: WA"
                     /collected_by="ARMADA"
     gene            complement(<1..152)
                     /locus_tag="I6K53_RS26455"
                     /old_locus_tag="I6K53_26455"
     CDS             complement(<1..152)
                     /locus_tag="I6K53_RS26455"
                     /old_locus_tag="I6K53_26455"
                     /inference="COORDINATES: protein motif:HMM:NF014457.4"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_071561715.1"
                     /translation="MKKLLFLGVVTVSVVLAACQVNNVRDTGGGAVSPSSTVTGVTLDS
                     GTNGNP"
                     /name="colicin release lysis protein"
     gene            complement(260..517)
                     /locus_tag="I6K53_RS26460"
                     /old_locus_tag="I6K53_26460"
     CDS             complement(260..517)
                     /locus_tag="I6K53_RS26460"
                     /old_locus_tag="I6K53_26460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013365855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="WP_025714833.1"
                     /translation="MAGNKISDFTEEEFTAFVSKIIAADFPTDKENDDAIYSFAQLTEH
                     PAGWNLIFKPKVGEDSSAKGIVKTVKEWRAANGKPGFKKA"
                     /name="bacteriocin immunity protein"
     gene            complement(519..>1121)
                     /locus_tag="I6K53_RS26465"
                     /old_locus_tag="I6K53_26465"
                     /pseudo=""
     CDS             complement(519..>1121)
                     /locus_tag="I6K53_RS26465"
                     /old_locus_tag="I6K53_26465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178051.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="klebicin B-related nuclease bacteriocin"
                     /name="klebicin B-related nuclease bacteriocin"
     gene            <1120..1461
                     /locus_tag="I6K53_RS26470"
                     /old_locus_tag="I6K53_26470"
                     /pseudo=""
     CDS             <1120..1461
                     /locus_tag="I6K53_RS26470"
                     /old_locus_tag="I6K53_26470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178052.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /name="hypothetical protein"
ORIGIN
        1 ggattgccgt tcgtaccgct atcaagcgtg actccggtca ccgtcgatga aggtgaaaca
       61 gccccacctc cggtgtctcg aacattattg acctgacatg ctgccagaac cacagaaacg
      121 gtaacaaccc ctaggaaaag taattttttc atatatcacc ttttattttt attattctag
      181 gcaaatggat atctagtaat tattcttcaa atccagagtt acaaatgagc gggctcataa
      241 cttgaacccg caaattcaat cacgcttttt taaaacctgg cttaccgtta gcagctcgcc
      301 attcctttac ggttttaaca attcctttcg ccgaagaatc ttcgcctacc ttaggcttga
      361 agatgaggtt ccaaccagca ggatgctctg ttagttgggc gaacgaataa atagcatcat
      421 cgttctcttt gtcagttgga aaatcagcag cgattatttt actaacaaaa gcagtaaatt
      481 cttcttccgt aaaatctgaa attttattac cagccatttt attttccttt atgtgtctca
      541 atatgatttt taggtgttag cgccttaatg ttatcgacat ccataacagc ccctccttga
      601 gatatttctt tttcgtggtg taattcaatt ttaacacgtt tccctactga ttctaaaaag
      661 tccgcagtag gtgcccttcc agctttcatc tgattaatat cagcttcact aaattgcttg
      721 ctcagtgcag aatcgtcagc aaccgctttc cagaacgccc gacggaaaga atcaaaacta
      781 ccgaaagtct tcccgcgtaa tttatccgca atctgggaag gaacaggagc accatccccg
      841 gtactggccc cccccatcca gttattgcca acgttctgcc ctttgccgct cactgtaccg
      901 ggcatgttac gagggctacg gtacatcaca tacagcggtt ttaggccgga ttccggagga
      961 aacaccagaa taatatcgtc aaaatcgagg tcatccgcta ccggcgtcac ggttttatct
     1021 gtagcctgtg gcgccgaaat ctgatcaccg gtatgcagaa tacgctccgg tagtggtacc
     1081 ggcccgctta gcgtcagtgg tccgtttgca ccaggtgcgt caagtgcact agatttcacc
     1141 aggatggcgc ttaatcagga aaaatacctc gatgacttta catggaaaga actgaacgac
     1201 gttatcaact tcatacgcaa agcagaaggt agtgcatctg accacagcta tgcactccag
     1261 ttactggaga ttaatttcaa ggcaaaccca ctggatctca tctatcaccc tgactgctgg
     1321 ttcaatgatg aagcattgtt tcatgcccgg ctgactaccg aagaaattgg tggctactta
     1381 atgaaaaaat caggtcgatg gctcaacgat gcaccggata tccaactggt gtatgccatc
     1441 ccccaggatg tttatgatta accaagtcac catttaccat aatcccttaa ttgtacgcac
     1501 cgctaaaacg gcctcaggac gtttctgacg ggaaaccggt aaaacaggca acaaaccacc
     1561 cgaaaacccg ccagaaacgc gcaggctctg ttttta
//
