LOCUS       NZ_CP069880             2107 bp    DNA     linear   CON 05-JAN-2024
DEFINITION  Klebsiella pneumoniae strain FDAARGOS_1323 plasmid unnamed6.
ACCESSION   NZ_CP069880
VERSION     NZ_CP069880.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN16357465
            Assembly: GCF_016903295.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 2107)
  AUTHORS   Sokurenko,E., Tallon,L., Sadzewicz,L., Zhao,X., Boylan,J., Ott,S.,
            Bowen,H., Vavikolanu,K., Mehta,A., Aluvathingal,J., Nadendla,S.,
            Yan,Y. and Sichtig,H.
  TITLE     FDA dAtabase for Regulatory Grade micrObial Sequences (FDA-ARGOS):
            Supporting development and validation of Infectious Disease Dx tests
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2107)
  AUTHORS   Sokurenko,E., Tallon,L., Sadzewicz,L., Zhao,X., Boylan,J., Ott,S.,
            Bowen,H., Vavikolanu,K., Mehta,A., Aluvathingal,J., Nadendla,S.,
            Yan,Y. and Sichtig,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2021) Institute for Genome Sciences, University of
            Maryland School of Medicine, BioPark II, 801 W. Baltimore St., Suite
            619, Baltimore, MD 21201, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SMRT v. 8.0.0, HGAP v. 4
            Genome Coverage                   :: 771.48x
            Sequencing Technology             :: Pacbio; Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/05/2024 07:40:22
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,312
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,080
            CDSs (with protein)               :: 5,080
            Genes (RNA)                       :: 124
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 13
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 48 of 108
            Pseudo Genes (incomplete)         :: 59 of 108
            Pseudo Genes (internal stop)      :: 15 of 108
            Pseudo Genes (multiple problems)  :: 14 of 108
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP069880.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..2107
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="FDAARGOS_1323"
                     /host="Homo sapiens"
                     /culture_collection="FDA:FDAARGOS_1323"
                     /db_xref="taxon:573"
                     /plasmid="unnamed6"
                     /geo_loc_name="USA: WA"
                     /collected_by="ARMADA"
     gene            complement(239..400)
                     /locus_tag="I6K53_RS26760"
     CDS             complement(239..400)
                     /locus_tag="I6K53_RS26760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_148593481.1"
                     /translation="MHEPPVQSDCCALSGNYHLESNPERHDKAPLAAATGNGFRGVGLE
                     VMRLLRLN"
                     /name="replication initiation protein"
     gene            805..996
                     /locus_tag="I6K53_RS26440"
                     /old_locus_tag="I6K53_26440"
     CDS             805..996
                     /locus_tag="I6K53_RS26440"
                     /old_locus_tag="I6K53_26440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_023302508.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLEKLNELDLDVEADLCEKLHDDA
                     EHLFRTLSSRLDELQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            996..1418
                     /locus_tag="I6K53_RS26445"
                     /old_locus_tag="I6K53_26445"
     CDS             996..1418
                     /locus_tag="I6K53_RS26445"
                     /old_locus_tag="I6K53_26445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_025714834.1"
                     /translation="MARVSISEAARLVKVSRPTIYKMINSGKLSYTSVVKHGKAIKVID
                     TSELIRVFGSLDGVIDTVKYDVKSDAESTSINSVGLHDLQHRIALLEAENDGLKGAVKA
                     RDEHIDSLRQAMQLLEHKREPSSPPHSPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     gene            1796..>2107
                     /locus_tag="I6K53_RS26450"
                     /old_locus_tag="I6K53_26450"
                     /pseudo=""
     CDS             1796..>2107
                     /locus_tag="I6K53_RS26450"
                     /old_locus_tag="I6K53_26450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001415424.1"
                     /note="incomplete; too short partial abutting assembly gap;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /name="hypothetical protein"
     Typing          complement(288..757)
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="90.041"
                     /coverage="92.0"
                     /evalue="5.88e-172"
ORIGIN
        1 gacaccggag gtggggctgt ttcaccttca tcgacggtga ccggagtcac gcttgatagc
       61 ggtacgaacg gcaatccgta aaggatcttc ttgagatccc atttggatcg tcgtaatctc
      121 ttgctctgta aacgaaaaaa ccgccttgga gggcggtttt tccgaaggtt caaacacttg
      181 caggtgattg aaccaaggta actggcttag aggagcgcag tcaccaaaac ttgttctttc
      241 agtttagcct taacaggcgc atgacttcaa gaccaactcc tctaaatccg ttaccagtgg
      301 ctgctgccag tggcgctttg tcgtgtcttt ccgggttgga ctcaagatga tagttaccgg
      361 ataaggcgca gcagtcggac tgaacggggg gttcgtgcat acagtccagc ttggagcgaa
      421 ctgcctaccc ggaactgagt gtcaggcgtg gaatgagaca aacgcggcca taacagcgga
      481 atgacaccgg taaaccgaat ggcaggaaca ggagagcgca cgagggagcc atcaggggga
      541 aacgcctggt atctttatag tcctgtcggg tttcgccacc actgatttga gcgtcaaatt
      601 ctgtgatgct tgtcaggggg gcggagccta tggaaaaacg gccgctgtgc tgccttctcc
      661 ctctccgagc tgtctgctct atgtcgttat gtgcgcgttg cgaccgctcg ccgcagccga
      721 acggcaggag cgtagcgact gagtgagcga ggaagcggaa aagagatctg tctgacattg
      781 agcactgacg ctctgaggcc ctttatgacg aaacaacaga aaacagcgct gaacatggct
      841 aaattcatcc agaatcagtc tctgctcctg ctggagaagc ttaacgagct tgatctcgat
      901 gttgaggccg acctttgtga gaaactccac gatgatgcgg agcatctttt ccgtacactg
      961 tcctcgagac tggatgaatt acaggatggc aattgatggc cagagtgtct attagtgaag
     1021 cggcacggct tgttaaggtt agccgtccca caatttacaa gatgattaac agtgggaagt
     1081 taagttacac ctctgttgta aagcacggga aggctattaa agttattgat acttcagagc
     1141 ttattagagt ttttggttct cttgatggtg tcatcgatac tgtaaaatat gatgtaaaaa
     1201 gtgacgcaga atctacaagt attaacagcg ttggtttaca tgatttacaa catagaatcg
     1261 ctctacttga agctgaaaac gatggtctta aaggggctgt taaggctcga gatgagcata
     1321 ttgattccct tcgtcaggct atgcagcttc tggagcacaa gcgcgagcct tcttcaccac
     1381 cccattcccc gtggtggaaa ttctggaaaa aatcctgatc ctgccatgct tgagttttcc
     1441 tctccggcca tcccagggag ttcctgaacc ttcccaaaaa tcgccagatc tctgaacgct
     1501 cctcacgact aaaaataccc acacactacg caaaattaaa attttgcaga ttccttattt
     1561 aaaacaattg gttaatgaaa aattaatgtg cagtgatgtg ttacaaagtg cagatccaag
     1621 tcgtggacga ctctatggcg aatcgtttac gaattgccca cgattcatat gtgaatcacg
     1681 ctttattatc atgttgttat gatgaatcga ctcttgattc aattcatcaa tttgggcgtt
     1741 ttttgttcat ggtgactctt gattcaattc acgattcgaa tcgccggtga atcgtgtggt
     1801 gagtcgtccg gcgacttgcc ataacgtgat tgccggccgt cgacgaaccc gattcctgaa
     1861 cgtgctgaaa accggttttc cggctgcgcc ggcaacgcct cacggccacg caataaattg
     1921 cgtccccgtt caccgttttc ggatcagaag aacgccggaa acaggactta ccctggctga
     1981 atggcgtgaa tttccgcgct atgcacttgc gcgcatactc atgcatgccg taaaaacaga
     2041 gcctgcgcgt ttctggcggg ttttcgggtg gtttgttgcc tgttttaccg gtttcccgtc
     2101 agaaacg
//
