LOCUS       NZ_CP069878             3818 bp    DNA     linear   CON 05-JAN-2024
DEFINITION  Klebsiella pneumoniae strain FDAARGOS_1323 plasmid unnamed3.
ACCESSION   NZ_CP069878
VERSION     NZ_CP069878.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN16357465
            Assembly: GCF_016903295.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 3818)
  AUTHORS   Sokurenko,E., Tallon,L., Sadzewicz,L., Zhao,X., Boylan,J., Ott,S.,
            Bowen,H., Vavikolanu,K., Mehta,A., Aluvathingal,J., Nadendla,S.,
            Yan,Y. and Sichtig,H.
  TITLE     FDA dAtabase for Regulatory Grade micrObial Sequences (FDA-ARGOS):
            Supporting development and validation of Infectious Disease Dx tests
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3818)
  AUTHORS   Sokurenko,E., Tallon,L., Sadzewicz,L., Zhao,X., Boylan,J., Ott,S.,
            Bowen,H., Vavikolanu,K., Mehta,A., Aluvathingal,J., Nadendla,S.,
            Yan,Y. and Sichtig,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2021) Institute for Genome Sciences, University of
            Maryland School of Medicine, BioPark II, 801 W. Baltimore St., Suite
            619, Baltimore, MD 21201, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SMRT v. 8.0.0, HGAP v. 4
            Genome Coverage                   :: 771.48x
            Sequencing Technology             :: Pacbio; Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/05/2024 07:40:22
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,312
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,080
            CDSs (with protein)               :: 5,080
            Genes (RNA)                       :: 124
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 13
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 48 of 108
            Pseudo Genes (incomplete)         :: 59 of 108
            Pseudo Genes (internal stop)      :: 15 of 108
            Pseudo Genes (multiple problems)  :: 14 of 108
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP069878.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..3818
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="FDAARGOS_1323"
                     /host="Homo sapiens"
                     /culture_collection="FDA:FDAARGOS_1323"
                     /db_xref="taxon:573"
                     /plasmid="unnamed3"
                     /geo_loc_name="USA: WA"
                     /collected_by="ARMADA"
     gene            504..2846
                     /locus_tag="I6K53_RS26405"
                     /old_locus_tag="I6K53_26405"
     CDS             504..2846
                     /locus_tag="I6K53_RS26405"
                     /old_locus_tag="I6K53_26405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010904246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-type pyocin domain-containing protein"
                     /protein_id="WP_181486822.1"
                     /translation="MGGGFNYNGEGATGTGLDRDPYVRDSNGNAIGVKSGYHAESYGTS
                     SPALGPNGAIQITAGVIAVPGDKPRPDGGSGGGNTVNTGPAGQLLVMNKGQLGYWETRS
                     TGAGNNEHNTSVFVAVGPSEAEKTASAEKALKEKQQAEAAAKDFAAKTAAASATAEKER
                     QQAIAAATAAGQHQSVSDARNSLNNATSDVSRLKSAADSALQEAKAKRKAAIDAVPVAT
                     QAENKYQELQQKIKGLKLKNGEYGTEKWEIIGSNKEHDHWGYRFYPSGITKAQVDVAQS
                     DAVNKRNQATSLASQATAAEQDSLKATAAYNAAETRRQAAQAALNSAEQAAAAERKRQE
                     AEAAAAAAAEKKRQADAVAKAAEEARAAAEKARLMQERQAAADKLKSTDIQSVRGIPST
                     ASPAASPISWAVASLGGISLDSVTAGKAWTQIAEVMAKLRGIAGASLVGPVVATAVGLF
                     WSRDVGIGSDVVPGRDISGLMPGDALSLPDLATLIKAADSKTGVSMPVRGRIIVREGDY
                     LESQFVRTPVAGSVPVVRATLDKATGYWGYTLPAIQGVPGQTILVSPSDAPGVNAPLGL
                     AGPVPLPETIIHTGGQTTVPQGGTVTVSPVIGEVDFRDLILVFPPESGLKPLYVMPVSP
                     YGDATEKGEHSGRPYNPDKAGGPVQNLNWRSAVINKAGIDKVKLHTGRFGESAANKVMI
                     DRLEKILSGQLQVTDTDKRFYTHELRELERYRALGVPDNVKPDDKGVTWNNTHTATLED
                     YKINEKTDPLYTKDAEEAEYKAELKNK"
                     /name="S-type pyocin domain-containing protein"
     gene            2857..3120
                     /locus_tag="I6K53_RS26410"
                     /old_locus_tag="I6K53_26410"
     CDS             2857..3120
                     /locus_tag="I6K53_RS26410"
                     /old_locus_tag="I6K53_26410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015377593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_064148700.1"
                     /translation="MSNPIEDIIKNESVSDVLRYFGPGRDISKIDRMYVSYKFEVISEG
                     VLLSEYKKLFDNGELKYDSNKNVIKGPNWKEPAFVTQKKYGI"
                     /name="hypothetical protein"
     gene            3302..3559
                     /locus_tag="I6K53_RS26415"
                     /old_locus_tag="I6K53_26415"
     CDS             3302..3559
                     /locus_tag="I6K53_RS26415"
                     /old_locus_tag="I6K53_26415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015366249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="WP_010904244.1"
                     /translation="MANKTLADYTEQEFIEFIEKIKKADFATESEHDEAIYEFSQLTEH
                     PDGWDLIYHPQAGADNSPAGVVKTVKEWRAANGKPGFKKS"
                     /name="bacteriocin immunity protein"
     gene            3667..>3818
                     /locus_tag="I6K53_RS26420"
                     /old_locus_tag="I6K53_26420"
     CDS             3667..>3818
                     /locus_tag="I6K53_RS26420"
                     /old_locus_tag="I6K53_26420"
                     /inference="COORDINATES: protein motif:HMM:NF014457.4"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_071561715.1"
                     /translation="MKKLLFLGVVTVSVVLAACQVNNVRDTGGGAVSPSSTVTGVTLDS
                     GTNGNP"
                     /name="colicin release lysis protein"
ORIGIN
        1 taaaaacaga gcctgcgcgt ttctggcggg ttttcgggtg gtttgttgcc tgttttaccg
       61 gtttcccgtc agaaacgccc tgaggccgtt ttagcggtgc gtacaattaa gggattatgg
      121 taaatggggg cgctcgggtg gcggaatacc ctctacacca agactttttg tacaggtctc
      181 tgtccgtcag tcacaccctt tttaagagca catatcgtta attttttttc tctgccgagc
      241 cgcgtcctgt ctgggttttg tcatttctat tacgcttaac ttaatgaaaa ttcaaaagat
      301 aattgtatct ctccgcgtgt tgacagagtt ctcatgtcag gagtagtatt caagtactgt
      361 atataaacac atgtatttat atacagtact tggtgtggca gagaactaat aacagcctcc
      421 ggatacaggt tcgaaggcga taaaccgcac accagattct gacaagcagg aaaacgataa
      481 aaataacaac aaaggagaga gttatgggtg gtggatttaa ctataacggg gaaggtgcta
      541 ctggcaccgg attggatcgt gatccatatg ttcgcgacag caatggtaat gctattggtg
      601 ttaaatcagg ctatcacgcg gagtcctatg ggacctcaag tccagcctta gggcctaatg
      661 gcgctattca gattactgct ggggttattg ctgtgcctgg agataagccc cgacctgacg
      721 gtggtagtgg tggtgggaat actgttaaca ccggaccggc aggacagctc ctggtgatga
      781 ataaaggtca gcttggatac tgggaaactc gttctacggg cgcgggtaac aatgagcata
      841 atacgagtgt atttgttgcc gtaggtcctt ctgaggcaga aaaaactgct tctgcagaga
      901 aggcgttaaa ggaaaaacag caggcagaag cagcagcaaa agactttgcg gctaaaactg
      961 ctgccgcatc agccacggcg gaaaaagaac gccagcaggc aattgctgct gcaactgcag
     1021 cgggtcagca tcagtcagtt tctgatgccc gtaatagcct gaataacgcg acgtcagatg
     1081 tgtctcgtct gaaatcagct gcagacagtg cactgcagga ggcaaaggca aaacggaagg
     1141 ctgctattga tgctgtacct gttgcaaccc aggcggaaaa taaatatcag gagctgcagc
     1201 agaagattaa aggcctgaag ctgaaaaatg gtgagtatgg tacggaaaaa tgggaaataa
     1261 ttggctctaa taaggagcat gatcactggg gatacaggtt ttatccatcc ggaattacca
     1321 aagctcaggt tgatgtggcg cagagcgatg ctgtgaataa gcgaaatcag gctaccagtc
     1381 ttgccagtca ggcaacagca gcagaacagg acagcctgaa agctacagct gcctataatg
     1441 cggcagaaac gcgccgtcag gctgctcagg cggcgttaaa ttctgctgaa caggctgctg
     1501 ctgctgaacg taagcggcag gaagctgagg cggctgcagc agctgctgct gagaaaaaac
     1561 gtcaggcaga cgcagtagca aaagcagcag aggaagcgcg tgcggcagcg gaaaaagcca
     1621 ggctgatgca ggagcgtcag gcagcagcag ataagctgaa atccacagat attcagtctg
     1681 ttcgcgggat cccgtctacg gcatcgcctg cagcgtcacc catttcctgg gccgttgcat
     1741 cacttggtgg tatatcgctg gatagtgtta ctgcagggaa agcctggacg cagattgctg
     1801 aggtgatggc taaactgcga ggtattgccg gtgcgagtct tgttggtccc gtggtggcaa
     1861 ctgctgtagg gctgttttgg tcacgtgatg ttggtattgg cagtgatgtg gtgcccggac
     1921 gtgacatcag cgggctgatg ccgggtgatg cactgtcatt acctgatctg gccactttga
     1981 ttaaagctgc tgacagtaaa acgggtgtca gtatgccggt tcgaggccgg attatcgtgc
     2041 gtgaaggcga ttatctggag tctcagttcg ttcgaacacc tgttgccggt agtgttccgg
     2101 ttgttcgggc tactctggat aaagctactg gttactgggg atatacgttg ccggcgatac
     2161 agggcgtgcc cggacagaca atactggtaa gtccgtcaga tgcgccgggc gttaatgctc
     2221 ctctgggact tgctgggccg gttcctttgc ctgaaactat tatacatacc ggtgggcaaa
     2281 ctacggtccc tcaggggggg actgtgacag tatctcctgt tattggtgaa gttgattttc
     2341 gagatcttat ccttgttttc cctcctgagt cagggttaaa gccgttatat gtgatgccag
     2401 ttagccctta tggagatgca acagaaaaag gcgagcacag cggaagacct tataatccgg
     2461 ataaggcagg tggtcctgta cagaatctta actggcgtag tgcagtcatc aacaaggccg
     2521 ggattgataa ggtgaagcta cataccggtc gctttggtga atcagcagca aacaaagtaa
     2581 tgatcgaccg tctggagaaa atcctgagcg gtcagttaca ggtaactgat acggacaagc
     2641 ggttttacac ccatgaactc agagagctgg aacgttatcg tgctttaggg gtccctgata
     2701 atgtgaagcc agatgataaa ggtgttacgt ggaataacac tcatacagca acgctggaag
     2761 attataaaat taatgaaaaa actgatccgc tctatacaaa ggatgctgaa gaggcagagt
     2821 ataaagcaga gctaaaaaat aaatgagggg gagagaatga gtaatccgat tgaagacatc
     2881 attaaaaatg aatcggtaag tgatgtgctt cgctactttg gtcctggtcg cgacatttca
     2941 aaaattgacc gtatgtatgt ttcatataag tttgaagtga tctctgaagg cgttctttta
     3001 tctgaataca aaaagttatt cgacaatgga gaactaaagt atgactctaa taaaaatgtt
     3061 attaaggggc ctaactggaa agagcctgct tttgtaacac aaaaaaaata cggcatttaa
     3121 agtgtgcata tgaagccctt tgaaaagagg gctttgatta aagctcacat atgaaaaggg
     3181 tttctttttt gagtggtcgt ggtttcgatg atactggcag catgtcaggt caacaatatc
     3241 tggggtaagc tgagtgcatt aaaacctaaa cgccatattg aaatccataa tgggaactga
     3301 aatggctaat aaaactttgg ctgactatac agagcaggaa tttattgagt ttatcgaaaa
     3361 aattaaaaaa gcagactttg ctactgagtc tgagcatgat gaggccattt atgagttcag
     3421 ccagttgact gaacatccag atggttggga tcttatttat catcctcaag caggagccga
     3481 taactctcct gctggtgttg taaaaacagt aaaagagtgg cgagcagcta acggtaagcc
     3541 aggttttaaa aaatcgtgat tgaatgtgcg ggttcaagtg atgaatccgc acatttttaa
     3601 ctctggattt gaataataat tactaggtat ccatttgctt agaataataa aaataaaagg
     3661 tgatatatga aaaaattact tttcctgggg gttgttaccg tttctgtggt tctggcggca
     3721 tgtcaggtca ataatgttcg tgacaccgga ggtggggctg tttcaccttc atcgacggtg
     3781 accggagtca cgcttgatag cggtacgaac ggcaatcc
//
