LOCUS       NZ_CP069877             1917 bp    DNA     circular CON 05-JAN-2024
DEFINITION  Klebsiella pneumoniae strain FDAARGOS_1323 plasmid unnamed7,
            complete sequence.
ACCESSION   NZ_CP069877
VERSION     NZ_CP069877.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN16357465
            Assembly: GCF_016903295.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 1917)
  AUTHORS   Sokurenko,E., Tallon,L., Sadzewicz,L., Zhao,X., Boylan,J., Ott,S.,
            Bowen,H., Vavikolanu,K., Mehta,A., Aluvathingal,J., Nadendla,S.,
            Yan,Y. and Sichtig,H.
  TITLE     FDA dAtabase for Regulatory Grade micrObial Sequences (FDA-ARGOS):
            Supporting development and validation of Infectious Disease Dx tests
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1917)
  AUTHORS   Sokurenko,E., Tallon,L., Sadzewicz,L., Zhao,X., Boylan,J., Ott,S.,
            Bowen,H., Vavikolanu,K., Mehta,A., Aluvathingal,J., Nadendla,S.,
            Yan,Y. and Sichtig,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2021) Institute for Genome Sciences, University of
            Maryland School of Medicine, BioPark II, 801 W. Baltimore St., Suite
            619, Baltimore, MD 21201, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SMRT v. 8.0.0, HGAP v. 4
            Genome Coverage                   :: 771.48x
            Sequencing Technology             :: Pacbio; Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/05/2024 07:40:22
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,312
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,080
            CDSs (with protein)               :: 5,080
            Genes (RNA)                       :: 124
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 13
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 48 of 108
            Pseudo Genes (incomplete)         :: 59 of 108
            Pseudo Genes (internal stop)      :: 15 of 108
            Pseudo Genes (multiple problems)  :: 14 of 108
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP069877.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1917
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="FDAARGOS_1323"
                     /host="Homo sapiens"
                     /culture_collection="FDA:FDAARGOS_1323"
                     /db_xref="taxon:573"
                     /plasmid="unnamed7"
                     /geo_loc_name="USA: WA"
                     /collected_by="ARMADA"
     gene            610..912
                     /locus_tag="I6K53_RS26395"
                     /old_locus_tag="I6K53_26395"
     CDS             610..912
                     /locus_tag="I6K53_RS26395"
                     /old_locus_tag="I6K53_26395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005161566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TM2 domain-containing protein"
                     /protein_id="WP_031942343.1"
                     /translation="MSSMVYCRGCGKEIHETAKSCPHCGATNASSGSGEKSRIAAALLA
                     FFLGGFGAHKFYLGKIGQGFLYLIFCWTFIPAIIAFIEFIIYLCDSDEKFARKYG"
                     /name="TM2 domain-containing protein"
     gene            complement(1172..1408)
                     /locus_tag="I6K53_RS26400"
                     /old_locus_tag="I6K53_26400"
     CDS             complement(1172..1408)
                     /locus_tag="I6K53_RS26400"
                     /old_locus_tag="I6K53_26400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001676031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_012772745.1"
                     /translation="MARLTNLTPAEKKFLDDAVAAAERASGKKLNQPNRHIVLNRARAQ
                     IELQRYADRQRALREDERQQSDFAWSRPRAPRR"
                     /name="hypothetical protein"
     Typing          complement(1574..1747)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="2.5600000000000002e-86"
     Typing          11..506
                     /name="rep_cluster_2358"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="84.0"
                     /coverage="99.0"
                     /evalue="6.33e-132"
     Typing          1720..1917
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="91.96"
                     /coverage="95.0"
                     /evalue="1.92e-72"
ORIGIN
        1 ctcaaatcag tggtggcgaa acccgatgcg gcttaaagat accaggcgtt tccccctggt
       61 ggctccctcg tgctcccccg cattcctgcc tttcggttta ccggtgtcat tccgctgtta
      121 tggccgcgtt tatctcattc cacgcctgac actcagttcc gcgaaggcag ttcgctcctg
      181 gatggactgt atgcacgaac cccccgttca gtccgactgc tgcgccttat ccggtaacta
      241 tcatcttgag tccaacccgg aaagacacga cttatcgcca ctggcagcag ccactggtaa
      301 ctgatttaga ggagttagtc ttgaagttat gcgcctgtta aggctaaact gaaagaacag
      361 atttggttac tgcgctcctc gtaagccagt taccacggtt caaagagttg ctgctctgag
      421 aaccttcgaa aaaccgcctt gcagggcggt tttttcgttt acagagcaag agattacgac
      481 gccacagaaa ggatctcaag aagatccttc accgtaatgg aaatctatcc aatccgagca
      541 cataggatac acatcgttat aaaacatcgg atattatgat cccaattaaa ataagctcga
      601 ggataaaaaa tgagttcaat ggtttactgc cgaggatgtg gcaaggaaat acacgaaaca
      661 gcaaagtcat gcccgcactg cggagccaca aacgcctctt caggttcagg tgaaaaaagt
      721 agaatcgcag ccgcgctatt agcattcttc cttggtggat ttggcgcaca taaattttac
      781 ttaggaaaaa taggacaggg gttcctgtat ctaatttttt gctggacatt catacccgcg
      841 atcatcgcgt ttattgagtt tattatttat ctatgcgatt ccgatgaaaa attcgcacga
      901 aaatatggct aactcatagc aaaaaaaagc cccctaaggg cttttttttg tacctgacgg
      961 tcagaccgat taaacataac atcccttgcc cgcacccccg caggcgccct cagcgcaatc
     1021 ctgctgcatc agggccgttt ccagcacctt tcctcaccag tatcccggaa aaacgcccct
     1081 gatgatttct gcgcgactgc atggctatgc acgcaggtga atggcaggtg atttcgcagc
     1141 gtcagcacca ctaaaccgcc aaaagccaaa atcagcggcg cggcgcgcgc ggacgtgacc
     1201 aggcaaaatc ggactgctgc cgctcatcct cacgtaatgc acgctggcgg tcggcgtaac
     1261 gctgcaactc aatttgcgca cgcgctctgt tcaggacgat gtgccggtta ggctgattta
     1321 gctttttacc tgacgctctt tcagctgcgg caacagcatc atcaagaaat tttttctctg
     1381 caggcgtcaa gtttgtcagt ctggccataa cgttctccag tcagaataaa tcagcacagt
     1441 ctgccagaca cagtagaaac gatgaagcgc cagcgacggt gaaaggcagt cagaaacggt
     1501 gaacgggagc ggattttaat ccggtgccgt gaggcgttgg acaagccgca ggcgcgtcat
     1561 tagcttttag cgggtttcgg ggcgcagccc tgaaccagtc acgtagcgct agcggagtgt
     1621 atactggctg actatgttgg cacggatgtg gaaatcagtg aagtgcttca tgtagcagga
     1681 gaaaaaaggc ggcaccagcg cgtcagcagg atatgtagta caggatatat tccgcttcct
     1741 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtgccg gcttacaggc
     1801 ggggcggaaa tttcctggga gataccagga aggagcttaa cagggaagag ataaggccgc
     1861 ggcgaagccg tttttccata ggctccgccc ccctgacaaa catcatgaaa tctgacg
//
