LOCUS       NZ_CP069876             3373 bp    DNA     circular CON 05-JAN-2024
DEFINITION  Klebsiella pneumoniae strain FDAARGOS_1323 plasmid unnamed4,
            complete sequence.
ACCESSION   NZ_CP069876
VERSION     NZ_CP069876.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN16357465
            Assembly: GCF_016903295.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 3373)
  AUTHORS   Sokurenko,E., Tallon,L., Sadzewicz,L., Zhao,X., Boylan,J., Ott,S.,
            Bowen,H., Vavikolanu,K., Mehta,A., Aluvathingal,J., Nadendla,S.,
            Yan,Y. and Sichtig,H.
  TITLE     FDA dAtabase for Regulatory Grade micrObial Sequences (FDA-ARGOS):
            Supporting development and validation of Infectious Disease Dx tests
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3373)
  AUTHORS   Sokurenko,E., Tallon,L., Sadzewicz,L., Zhao,X., Boylan,J., Ott,S.,
            Bowen,H., Vavikolanu,K., Mehta,A., Aluvathingal,J., Nadendla,S.,
            Yan,Y. and Sichtig,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2021) Institute for Genome Sciences, University of
            Maryland School of Medicine, BioPark II, 801 W. Baltimore St., Suite
            619, Baltimore, MD 21201, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SMRT v. 8.0.0, HGAP v. 4
            Genome Coverage                   :: 771.48x
            Sequencing Technology             :: Pacbio; Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/05/2024 07:40:22
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,312
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,080
            CDSs (with protein)               :: 5,080
            Genes (RNA)                       :: 124
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 13
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 48 of 108
            Pseudo Genes (incomplete)         :: 59 of 108
            Pseudo Genes (internal stop)      :: 15 of 108
            Pseudo Genes (multiple problems)  :: 14 of 108
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP069876.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3373
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="FDAARGOS_1323"
                     /host="Homo sapiens"
                     /culture_collection="FDA:FDAARGOS_1323"
                     /db_xref="taxon:573"
                     /plasmid="unnamed4"
                     /geo_loc_name="USA: WA"
                     /collected_by="ARMADA"
     gene            8..568
                     /locus_tag="I6K53_RS26370"
                     /old_locus_tag="I6K53_26370"
     CDS             8..568
                     /locus_tag="I6K53_RS26370"
                     /old_locus_tag="I6K53_26370"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_000067486.1"
                     /translation="MSNLLQMGTDFEKKLKERAASTENMLNSEFRKLEESVDKALSLNR
                     QKIRDAISEHTTSVKKQLDTLSTTVSMQFSTTEAELSRQQKKLLWRVIKGRILFPALTA
                     LSVTGGIFLGCWGLMEWQESKIAKNILTIREQENTLAKLEAKTWGVTFVNGENGKFLVL
                     PDGMKGENTWTVGDKNAVRLVRE"
                     /name="MbeB family mobilization protein"
     gene            575..808
                     /locus_tag="I6K53_RS26375"
                     /old_locus_tag="I6K53_26375"
     CDS             575..808
                     /locus_tag="I6K53_RS26375"
                     /old_locus_tag="I6K53_26375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137389.1"
                     /translation="MTELEGHLLNALEHLQQDYMRRLNEWESAFAELQKMHEVTQRNNA
                     ILNERVVILSQQVQRLAGQVDRLSRLFIANNS"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(2125..2316)
                     /locus_tag="I6K53_RS26380"
                     /old_locus_tag="I6K53_26380"
     CDS             complement(2125..2316)
                     /locus_tag="I6K53_RS26380"
                     /old_locus_tag="I6K53_26380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165979.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNDLDADEQADICESLHDHA
                     DELYRSCLARFGDDGESN"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            2365..2703
                     /locus_tag="I6K53_RS26385"
                     /old_locus_tag="I6K53_26385"
     CDS             2365..2703
                     /locus_tag="I6K53_RS26385"
                     /old_locus_tag="I6K53_26385"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_266097495.1"
                     /translation="MKREKMLTIRVTDEEHARLLERCEGKRLAEWMRRVCLGEPVARTG
                     KLPTLAPPLLRHLAAIGNNLNQTARKVNSGQWSSIDRVHVVAALMAIEGELRQLRQAVR
                     EQGVRDDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     Typing          1962..2044
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="92.857"
                     /coverage="99.0"
                     /evalue="1.38e-25"
     Typing          2..891
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="87.748"
                     /coverage="100.0"
                     /evalue="8.33e-145"
     Typing          2694..3373
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="94.273"
                     /coverage="100.0"
                     /evalue="6.69e-143"
ORIGIN
        1 gcgctgtatg agcaatcttt tacaaatggg aacagacttc gagaagaaac tgaaagagcg
       61 agccgcttct accgagaaca tgctgaacag cgagttcagg aagctcgaag agtctgtaga
      121 caaggcattg agcttaaaca ggcagaaaat cagagacgct atcagcgaac atacaacatc
      181 cgtgaaaaag caactggata cgctgagtac caccgtgagc atgcagttca gcaccacaga
      241 ggccgaactg tcccggcagc agaaaaaact gctctggcgg gtgatcaaag ggcggatact
      301 gtttccggcg ctgaccgcgc tcagcgtcac gggcgggatt ttcctggggt gctgggggct
      361 gatggagtgg caggaaagca agattgcgaa aaatatcctg actatcagag agcaggaaaa
      421 cacgctggcg aaactcgagg caaaaacctg gggggtgacg ttcgtcaatg gcgagaacgg
      481 gaaatttctg gtgttgccgg acgggatgaa aggggaaaac acctggacgg tgggcgacaa
      541 gaacgccgtg aggctggtca gggagtaaac gagtatgacg gaactggaag ggcatttgct
      601 gaacgcctta gagcatttgc aacaggacta tatgcggcgg ctgaacgagt gggagagcgc
      661 cttcgcggaa ttgcagaaga tgcacgaggt tacgcaacgg aacaacgcga tactgaacga
      721 gcgcgtcgtg atattgagtc agcaggtgca gagattagcc gggcaagtgg atcgcttgag
      781 tcggttattt atcgcgaaca acagctaagg gatgaacagg agcgtgcccg gtacagacat
      841 gaggtacagg aacgcgcggc acgggttcgg agtgggccat caatggagat gtgaccgggg
      901 aagtgacgaa atcttgccat tttgacccgt tcgacggctg gggcgagcga aacaaatcgt
      961 gtgccgtatt tgtgcatagc tatgcaacat cctgaaaacg catttcacgc gtttttgggg
     1021 attcagccag gggaaaacat ggttttttta ctttttgagc gtaaagacgt gaaaaatctc
     1081 gttattgcac ttgcgatctt tcgtcggcgg gcgtagaacg aaaacagctc gccaggaagg
     1141 cgagctgttt cgggttcatt gaggtctctg acctcctcaa tgttcctgtg gaaactgttg
     1201 agatccacaa cagaaaccag ttcattcagt ttgcatgagc cttctgtatt cttcaagaat
     1261 ttttcaacag cacagtctcc caggaacccc gaaaaaaacc tgtttttgca gatacttgcg
     1321 tatttgcgag aatcgtttgc ggttggggga gctgtgacga cggcgaccgc acgcgtgaga
     1381 gttccgctac cggcgcagag agtttcatcg acagcggaca cctgatgagt tgccacaaaa
     1441 accgtatgta gcgcggctag attggttgag ttagaccttg gcggcagttc ccggtatgtg
     1501 gtggcgtgac gggtgagatg gtgcggcgaa gacgcatacg tcacgcaggc gcgggttctt
     1561 ccactgacgc gccccgtgat cgatggcctt ggctccgtcc ggcatatcga gagcggcaaa
     1621 ctacccgtcc cttcactggg aggggtgagt tgttgccgtt ccctccgacc ttccaccatc
     1681 cagcatatcg cacgatccca tgtgcaccgc tactcctgat aactccagtg caaaaaaaaa
     1741 aaacggatct gcatcacaac gagagtgatc ccaccgctcg ccgcagcccc agcgaccgaa
     1801 cgtagtgagc gagtgggcga ggaagcggaa gagtcacaga ggcaagcatt gtgcacttac
     1861 gcgcctgtgc cagtgtccgc gtgtgtgttg tttatcgggc gcgctgtttt tacgcatctg
     1921 tgcggctttt tactcctgac atggtgcact tcccatccac tctgcaccga tgcaaaatga
     1981 ggtaagccag tatacactcc gctatcgcta cgtgactggg tcagggcttc gccccgacac
     2041 ccgcactgac gcgccctggc gggcttgtct gctcccggca tccgctcaca ggcaagctgt
     2101 gaacgtctcc gggagctgca tgtttcagtt gctttcaccg tcatcgccga agcgtgcgag
     2161 gcaactgcgg tagagttcgt cggcgtggtc gtgaagtgat tcgcagatgt ctgcctgttc
     2221 gtccgcgtcc agatcgttga gtttttccag cagggttagc gtctggcttc tgataaagcg
     2281 agccatgtta agggctgttt tttcctgttt ggtcatggtc ttaaggctcc ttgtatgggg
     2341 gatttgttag ggggaatgat accgatgaaa cgcgagaaga tgctcacgat acgggttacc
     2401 gatgaggagc acgcccggtt actggaacgc tgcgagggta aacgactggc ggaatggatg
     2461 cggcgggtct gtctggggga gccggttgca cgcacaggaa aactgccgac actggcaccg
     2521 ccattgctgc ggcatctggc tgccatcggg aacaacctga accagacagc ccggaaggtg
     2581 aacagcggcc agtggtcttc gattgaccgg gttcatgtgg tggccgcact gatggccatc
     2641 gagggtgaat tgcgccagtt gcggcaggct gtcagggaac agggggtacg ggatgatagt
     2701 taaatttcat gccaggggga aaggcggtgg aagtggcccg gtcgattacc tgctggggcg
     2761 ggagcgtaac cgcgaaggcg caacggtgct ccggggtaac ccggaagaag tgcgggaact
     2821 cattgattcc acgccatttt cgaagaaata cacgtcaggc gttctgtcgt tcgcggagaa
     2881 ggagctacca ccgggtgaac gcgaaagagt gatgacgagc tttgagcgtg ttcttatgcc
     2941 cggtctcgaa aaagaccagt acagcattct ctgggtggaa caccaggata aggggcggct
     3001 cgaactgaat tttgtcatcc cgaacatgga gctacagagc ggtaagcgcc tccagccgta
     3061 ctacgaccgt gcggacaggc cgaggataaa tgcctggcag acgctggtta atcatcatta
     3121 cgggctgcgt gacccgaacg caccggagaa cagacggacg cttgtcacgc caaataatct
     3181 gccaaaagcg aaacaggagg ccgcagaagc gattacacgg ggtcttgagg ctctttacca
     3241 tgccggagca ataaaaacgc gtcaggatgt cacagaggcg cttacagcgg caggttttga
     3301 ggtggtgagg acaaccaaaa gcagcatcag tattgccgac ccggaggggg ggcggaatct
     3361 caggctaaag ggg
//
