LOCUS       NZ_CP069872             2955 bp    DNA     linear   CON 17-JUN-2023
DEFINITION  Escherichia coli strain FDAARGOS_1282 plasmid unnamed8.
ACCESSION   NZ_CP069872
VERSION     NZ_CP069872.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN16357424
            Assembly: GCF_016903275.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2955)
  AUTHORS   Sokurenko,E., Tallon,L., Sadzewicz,L., Zhao,X., Boylan,J., Ott,S.,
            Bowen,H., Vavikolanu,K., Mehta,A., Aluvathingal,J., Nadendla,S.,
            Yan,Y. and Sichtig,H.
  TITLE     FDA dAtabase for Regulatory Grade micrObial Sequences (FDA-ARGOS):
            Supporting development and validation of Infectious Disease Dx tests
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2955)
  AUTHORS   Sokurenko,E., Tallon,L., Sadzewicz,L., Zhao,X., Boylan,J., Ott,S.,
            Bowen,H., Vavikolanu,K., Mehta,A., Aluvathingal,J., Nadendla,S.,
            Yan,Y. and Sichtig,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2021) Institute for Genome Sciences, University of
            Maryland School of Medicine, BioPark II, 801 W. Baltimore St., Suite
            619, Baltimore, MD 21201, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SMRT v. 8.0.0, HGAP v. 4
            Genome Coverage                   :: 976.61x
            Sequencing Technology             :: Pacbio; Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 06/17/2023 05:21:51
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,162
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 124
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 14
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 65 of 186
            Pseudo Genes (incomplete)         :: 127 of 186
            Pseudo Genes (internal stop)      :: 33 of 186
            Pseudo Genes (multiple problems)  :: 37 of 186
            Pseudo Genes (short protein)      :: 4 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP069872.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..2955
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="FDAARGOS_1282"
                     /host="Homo sapiens"
                     /culture_collection="FDA:FDAARGOS_1282"
                     /db_xref="taxon:562"
                     /plasmid="unnamed8"
                     /geo_loc_name="USA: WA"
                     /collected_by="ARMADA"
     gene            complement(<1..115)
                     /locus_tag="I6K12_RS25535"
                     /old_locus_tag="I6K12_25540"
     CDS             complement(<1..115)
                     /locus_tag="I6K12_RS25535"
                     /old_locus_tag="I6K12_25540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_149822489.1"
                     /translation="MNKQQQAVLNMAGFIKSQSLTLLEKLDALDADEQAAMC"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            399..722
                     /gene="mobA"
                     /locus_tag="I6K12_RS25540"
                     /old_locus_tag="I6K12_25545"
     CDS             399..722
                     /gene="mobA"
                     /locus_tag="I6K12_RS25540"
                     /old_locus_tag="I6K12_25545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052604.1"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization protein MobA"
                     /protein_id="WP_000166966.1"
                     /translation="MTKRSGSNTRRRAISRPVRLTAEEDQEIRKRAAECGKTVSGFLRA
                     AALGKKVNSLTDDRVLKEVMRLGALQKKLFIDGKRVGDREYAEVLIAITEYHRALLSRL
                     MAD"
                     /name="mobA"
     gene            complement(947..1366)
                     /locus_tag="I6K12_RS25545"
                     /old_locus_tag="I6K12_25550"
     CDS             complement(947..1366)
                     /locus_tag="I6K12_RS25545"
                     /old_locus_tag="I6K12_25550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="WP_001254967.1"
                     /translation="MRRTGNKLCLIAMITATVALTACTPKGSVEQHTRHYVYASDDGFD
                     PNFSTQKADTTRMMVPFFRQFWDMGAKDKATGKSRSDVQQRIQQFHSQEFLNSLRGTTQ
                     FAGTDYRSKDLTPKKSRLLADTISAVYLDGYEGRQ"
                     /name="Exc2 family lipoprotein"
     gene            complement(1447..1884)
                     /locus_tag="I6K12_RS25550"
                     /old_locus_tag="I6K12_25555"
     CDS             complement(1447..1884)
                     /locus_tag="I6K12_RS25550"
                     /old_locus_tag="I6K12_25555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001749514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="entry exclusion protein 1"
                     /protein_id="WP_195390224.1"
                     /translation="MGHCNFMAWHTLRDALKMTGRSRSQFYRDMRSGLVSYRTSQDGRR
                     EFETSELIRAYGELKRNETPERHTQGHSEKPHDQQTERILRELNELKQCLTLMLEDKQA
                     QDTERRRQEAERKQLQNEIAQFRQALEQEKKRGFWSRLFGH"
                     /name="entry exclusion protein 1"
     gene            2144..>2955
                     /locus_tag="I6K12_RS25555"
                     /old_locus_tag="I6K12_25560"
                     /pseudo=""
     CDS             2144..>2955
                     /locus_tag="I6K12_RS25555"
                     /old_locus_tag="I6K12_25560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447006.1"
                     /note="incomplete; too short partial abutting assembly gap;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-10"
                     /name="colicin-10"
     Typing          151..390
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="99.585"
                     /coverage="100.0"
                     /evalue="3.54e-121"
     Typing          400..719
                     /name="MOBF"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.065"
                     /coverage="100.0"
                     /evalue="1.68e-62"
ORIGIN
        1 cacacatggc agcctgttcg tcagcatcga gtgcgtccag cttttcgagc agcgtcaggc
       61 tctggctttt tatgaatcct gccatgttga gtacagcttg ctgctgcttg ttcatctttt
      121 cgttttctcc gttctgtctg tcatctgcgt tgtgtgatta tatcgcgtac cacttttcga
      181 ctgttttacc gccggtattc tgccgtctga cgctttgacg ggtatttctg cctgacaata
      241 ctgtcactgc caaaaaactg ccgtgccttt gtcggtaatt cgaacttgct gacaggacag
      301 gatgtgtaat ttttataccg cgcatacatg cacgctatta caattaccct ggtcagggct
      361 ttgccccgac acccatgtca gatacggagc catgttttat gacaaaacga agtggaagta
      421 atacgcgcag gcgggctatc agtcgccctg ttcgtctgac ggcagaagaa gaccaggaaa
      481 tcagaaaaag ggctgctgaa tgcggcaaga ccgtttccgg ttttttacgg gcggcagctc
      541 tcggtaagaa agttaactca ctgactgatg atcgggtact gaaagaagtt atgagactgg
      601 gggcgttaca gaaaaaactc tttatcgacg gcaagcgtgt cggggacagg gagtatgcgg
      661 aggtgctgat cgctattacg gagtatcacc gtgccctgtt atccaggctt atggcagatt
      721 agcttcccgg agagaaaact gtcgaaaact gacggtatga acaccgtaag ctcccaaagt
      781 gatcaccatt cgctttcatg catagctatg cagagagctg aaaacgatcc tgacgcatcc
      841 ttcctgtttt tccggggtaa aacatctctt tttgcggtgt ctgcgtcaga atcgcgttca
      901 gcgcgtttca gtggtgcgta caattaaggg attatggtaa atccacttac tgtctgccct
      961 cgtagccatc gagataaacc gcagaaatcg tgtcagccag cagcctggat tttttcgggg
     1021 taaggtcttt gctgcggtaa tcagtacccg caaattgagt tgtgccccgg agtgagttta
     1081 aaaattcctg gctgtgaaac tgttgaatgc gttgttgcac atcactccgt gattttcctg
     1141 tcgctttatc tttagctccc atatcccaga actgccgaaa aaaaggcacc atcattcgtg
     1201 ttgtgtcggc tttttgggtg gaaaagttgg gatcaaaacc gtcatcagaa gcatatacgt
     1261 aatgccgggt atgttgttcc acgctgccct ttggggtaca ggctgtgaga gctactgttg
     1321 ctgttatcat ggcgataaga caaagtttgt ttccggtacg tctcatgagt gtctccctac
     1381 cctaaagtat ttattctcgg tgagttatta tccgaagatt atcttatcat cagtttctga
     1441 gcgcgttcag tgaccgaaca gcctggacca gaatccccgt ttcttttcct gctccagagc
     1501 ctgcctgaac tgggctatct cattctgtag ctgtttccgt tctgcttcct ggcgccggcg
     1561 ctccgtatcc tgggcttgtt tatcctcaag catcagcgtc aggcattgtt tcagctcatt
     1621 cagttcccgg agaatgcgtt ctgtctgctg atcatgaggt ttttctgagt gtccttgagt
     1681 gtgcctttct ggtgtctcat tccgctttaa ttcgccgtat gcccggatca gttcactggt
     1741 ttcaaactcc cgtcgtccgt cctggctggt acggtaactg acaagacctg agcgcatatc
     1801 acggtaaaac tgtgaacgcg atcttccagt cattttcagt gcgtcccgga gtgtgtgcca
     1861 tgccataaag ttacagtgtc ccataggtgt ctcatctcat ggtttcagta aaacataatg
     1921 aggtctgaga acggtaatgt ttgtgctggt ttttgtggca tcgggtggaa atagcacgtg
     1981 gtgtgggtgg ctgttttttt gatcgttttc acaaaaatga aaatccacag ggttgacagg
     2041 gaaaatgcag cggcgtaatt ttaaactgta tataaaacca gtgattatat gtacagtata
     2101 ttgcttttaa ctatttgttt ttaatgtcaa agaggatttt ataatggaaa cagctgtagc
     2161 gtactataaa gatggtgttc cttatgatga taaggggcag gtaatcatta ctcttttgaa
     2221 tggtaatcca gacgggagtg gctctggcgg tggtggtgga actggaggta gcaaaagtga
     2281 aagttctgca gccattcatg ccacagctaa atggtctact gctcaattga agaaaacgca
     2341 ggcagaacag gctgcccgag caaaagctgc cgcagaagca caggctaaag caaaagcaaa
     2401 ccgggatgcg ctgactcaac atctgaagga tattgtgaat gaggcgcttc gccataattc
     2461 cactcatccg gaggttattg acctggctca tgccaataat gcagcgatgc aggcagaagc
     2521 agagcggttg cgccttgcaa aagcagaaga aaaagcccgt aaagaagcgg aagctgcgga
     2581 aaaggctttt caggaagcag aacaacgacg taaagagata gagaaggagc aggctgaaac
     2641 agaacgccaa ttgaaactgg ctgaggatga agagaaacgc ctggcagcat tgagtgaaga
     2701 ggcccgggct gtggaggtgg cacaaaaaaa tcttgctgct gcacaatctg agctggcgaa
     2761 agtggatgaa gagattaata cgctcaatac ccgtttaagc tccagtattc atgcccgtga
     2821 tgcagaaacg aatacgctgt ccggaaaacg aaatgagttg gatcaggcat ctgctaaata
     2881 taaagaactg gatgaaaggg ttaaactttt atctcctagg gcgaatgatc cgcttcagag
     2941 tcgtcctttt tttga
//
