LOCUS       NZ_CP069870             5167 bp    DNA     circular CON 17-JUN-2023
DEFINITION  Escherichia coli strain FDAARGOS_1282 plasmid unnamed6, complete
            sequence.
ACCESSION   NZ_CP069870
VERSION     NZ_CP069870.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN16357424
            Assembly: GCF_016903275.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5167)
  AUTHORS   Sokurenko,E., Tallon,L., Sadzewicz,L., Zhao,X., Boylan,J., Ott,S.,
            Bowen,H., Vavikolanu,K., Mehta,A., Aluvathingal,J., Nadendla,S.,
            Yan,Y. and Sichtig,H.
  TITLE     FDA dAtabase for Regulatory Grade micrObial Sequences (FDA-ARGOS):
            Supporting development and validation of Infectious Disease Dx tests
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5167)
  AUTHORS   Sokurenko,E., Tallon,L., Sadzewicz,L., Zhao,X., Boylan,J., Ott,S.,
            Bowen,H., Vavikolanu,K., Mehta,A., Aluvathingal,J., Nadendla,S.,
            Yan,Y. and Sichtig,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2021) Institute for Genome Sciences, University of
            Maryland School of Medicine, BioPark II, 801 W. Baltimore St., Suite
            619, Baltimore, MD 21201, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SMRT v. 8.0.0, HGAP v. 4
            Genome Coverage                   :: 976.61x
            Sequencing Technology             :: Pacbio; Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 06/17/2023 05:21:51
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,162
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 124
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 14
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 65 of 186
            Pseudo Genes (incomplete)         :: 127 of 186
            Pseudo Genes (internal stop)      :: 33 of 186
            Pseudo Genes (multiple problems)  :: 37 of 186
            Pseudo Genes (short protein)      :: 4 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP069870.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5167
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="FDAARGOS_1282"
                     /host="Homo sapiens"
                     /culture_collection="FDA:FDAARGOS_1282"
                     /db_xref="taxon:562"
                     /plasmid="unnamed6"
                     /geo_loc_name="USA: WA"
                     /collected_by="ARMADA"
     gene            join(5111..5167,1..342)
                     /locus_tag="I6K12_RS25490"
                     /old_locus_tag="I6K12_25495"
     CDS             join(5111..5167,1..342)
                     /locus_tag="I6K12_RS25490"
                     /old_locus_tag="I6K12_25495"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001222912.1"
                     /translation="MRISNIEWLKKRIGFIRKLGEQTARQRQIIDLIDNEAGLTEQERK
                     LLHVLATAEKNDLQAQESERKQAVQKRIEGKKQRRERNHQLFLAAGLLIEAGLVDTKTG
                     ELCYKKDRILQALKELKYDLETSPNPDA"
                     /name="hypothetical protein"
     gene            complement(546..1460)
                     /locus_tag="I6K12_RS25495"
                     /old_locus_tag="I6K12_25500"
     CDS             complement(546..1460)
                     /locus_tag="I6K12_RS25495"
                     /old_locus_tag="I6K12_25500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_001271346.1"
                     /translation="MSAALQYFDENLPHRPYHTDDLAFGLRISGKGRALLARYIQQNQP
                     HAQFWLVFDVDREGAAIDWSDRNAPAPNITVKNPVNGHAHLLYALNIAVRTAPDASVKA
                     LKYAAAVERSLCEKLCADVNYSGLICKNPFHLEWLVMEWREEAYTLDELADYLDLSASA
                     RRSIDKHYGMGRNCHLFEMTRKWAYRAIRQGWPAFSQWLEAVIQRVEMYNASLPVPLSL
                     AECRAIGKSIAKYTHRNFTPETFAQYVADTHTPEIQAARGRKGGSKSKRSTVATSARTL
                     KPWEALGISRAWYYQLKKRGLVE"
                     /name="replication initiation protein"
     gene            3197..3406
                     /locus_tag="I6K12_RS25500"
                     /old_locus_tag="I6K12_25505"
     CDS             3197..3406
                     /locus_tag="I6K12_RS25500"
                     /old_locus_tag="I6K12_25505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001618649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001096196.1"
                     /translation="MAKLGENTPHIIDATVDFMASSQAFREYLKKLPPDNTIPPALTDE
                     HVPLYIRRLEYYRLLYRPKQTESY"
                     /name="hypothetical protein"
     gene            complement(3472..4005)
                     /locus_tag="I6K12_RS25505"
                     /old_locus_tag="I6K12_25510"
     CDS             complement(3472..4005)
                     /locus_tag="I6K12_RS25505"
                     /old_locus_tag="I6K12_25510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000168770.1"
                     /translation="MASTEEKLLTLLLSVEHLQHTAMEQQKAVADSLAVARETLRGYRD
                     ISDGVAKQVRDEVRNEVQKLDVAGLIGERISQDFQRLEESVANMKANADALNRDIATIH
                     SNIVQEYNTLKGYSWKWLGGVSVAFLVMFCVLGWGMKTILDRNYNATAAVYQKVVTLED
                     KLVEKPVTGKSRKN"
                     /name="hypothetical protein"
     gene            complement(4008..4934)
                     /locus_tag="I6K12_RS25510"
                     /old_locus_tag="I6K12_25515"
     CDS             complement(4008..4934)
                     /locus_tag="I6K12_RS25510"
                     /old_locus_tag="I6K12_25515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="WP_001331297.1"
                     /translation="MATYHLSVKFGGKGQAANHADYIERKEKYRDRQDLEYSAHGNMPE
                     WARDNPSHFWQAADQFERANGSTYRELEIALPRELTPEQRLELVQDFVRQEAGERHAWS
                     FAIHNPKASIDGGEQPHAHIMMSQRVNDGIERTPEQYFRRYNARYPERGGAKKDSGSLT
                     LTQQKEQLRELRKRWEVKHNEHMRKHGFERGFIDCRTLKEQGIERRPEVHLGFEAAGRL
                     DDAQRHDIMQKRSEPDYSRHLEQARTVGHDIREWLDTPVKQHELVRRLQHDVAREHGFD
                     MRDRVSRQNRTAPERVKSPSRGFSMGF"
                     /name="MobA/MobL family protein"
     Typing          complement(686..1460)
                     /name="Col156"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="96.907"
                     /coverage="83.0"
                     /evalue="0.0"
     Typing          complement(4012..4934)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="98.052"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 ggagaacaga ccgcacgaca gagacagatt attgacctga tcgacaacga agccggactc
       61 acagaacagg aacgcaaact tctccacgta ctggcaacag cggaaaaaaa cgacttacag
      121 gcacaggaga gcgaacgaaa acaggcggtc caaaaacgaa tcgagggaaa aaaacaacga
      181 cgggaacgca atcaccagct ctttctcgcg gctgggctac tgatcgaagc aggactggta
      241 gacacaaaaa caggcgagct atgctataaa aaagacagga tattacaagc acttaaggaa
      301 cttaagtacg atctggagac atccccaaac ccagacgcgt aagtgcctcc atccgtgttc
      361 cgattccctg gtgcttcctc gctcattcgc tcgctacgct cggtcatgag actgcggcga
      421 gcactaccac cacaatcacg cggaaagccg cgccattgca gggctgaacg gggaaaaacg
      481 gcaaaaaacc tgctgctcaa aaagacgcca gaaaggcgct gttatctgat ataggcttat
      541 ttggtctact ctacgagacc tcgttttttc agttggtaat accaggcgcg actgatccct
      601 aatgcctccc acggtttcag cgtgcgtgca gatgtagcta ctgtgctgcg cttagactta
      661 gaaccgccct tgcgaccgcg tgcagcctga atttctggcg tgtgcgtatc agccacatac
      721 tgtgcgaaag tttccggcgt gaagttcctg tgcgtgtatt tcgcaatgct cttgccaata
      781 gcccgacatt cagccagtga aagcggaacg ggaagcgatg cattgtacat ttcgacacgc
      841 tggatcaccg cttcaagcca ctgtgagaac gctggccagc cctgacgaat cgccctgtaa
      901 gcccatttgc gcgtcatttc gaacaggtgg cagtttcgcc ccatcccgta atgtttatcg
      961 atgctacgac gcgctgaggc gctcaaatcg agataatcag ccagttcatc gagggtatag
     1021 gcttcctcgc gccactccat caccagccat tccaggtgga acggattttt gcaaatcagg
     1081 ccgctgtagt tcacatccgc acaaagtttc tcacacagcg aacgttcaac cgctgcggca
     1141 tatttcagcg ccttaaccga ggcatcaggc gcggttctca cggcgatgtt gagtgcgtag
     1201 agcaggtgag cgtgtccgtt cacaggattt ttaacggtga tgttcggcgc gggagcgttc
     1261 cggtcgctcc agtcaatcgc agccccctcg cggtcaacgt caaaaaccag ccagaactgc
     1321 gcatgaggct ggttctgctg tatgtaccgc gcaagaagcg cacgcccttt gccggagatg
     1381 cgaagaccaa aagcgagatc atccgtgtga tagggacgat ggggtaaatt ttcgtcgaag
     1441 tattgaagcg cggcgctcat gtgttacgat tctctccggg ttatgcgacc tacacgccct
     1501 ctgtatttca tgttgaatct tgccggatct ggacatgatt tcaggggcgt ttgtttttca
     1561 gtcgcttaca ctacacccaa cggcaaatcc tgtcgaacct tcccgaaaac ccgatccatt
     1621 taccataact cccgttgtac gtacccatta aaacgccctc aatcgctttc tggcgcaaaa
     1681 aacgcaaaaa gacaagaaaa caccaggcca aaccagaaaa acgcgtcaga atcgttttca
     1741 gggcgttgca taactatgca cgaaagcgat gtgcgatcgg tttcgtacgt tctgaacacc
     1801 gaacggctca aaacgacaag ttttctcccc tggtgcgtgg cgctctgacg gggtttcctg
     1861 tttccaaact tcacatctcg ccaggacaaa aaaaaaccga cctggggtcg gtggcaagaa
     1921 tcagagtcta tgggaaatat tttagttatc ggatgctcaa tattcacaaa cgcaaaaaat
     1981 gcctcagtga tgccctaaaa caacctttcc agaaggagtc cgcgctttac gatgcacgga
     2041 agagataaaa aaccccacaa aagtggggaa ccaagagaaa caacaattac aggttgttgg
     2101 ttgtgttcga acaacgatga agtagctacg ctaaataata attaccgtac aggccctaca
     2161 tattcaacac taaatttaag atcctgacga aggatttata aatgcacgaa aacacataga
     2221 cagacaaatt cactaaattc atacacaaaa acaagtgtag ttttctaagt aaattagatt
     2281 cgacaaccaa ttagtttatc cacatcgcat tccactcttg ctacaccaat cacatttctt
     2341 gatatgtaat agcagaaaaa ataaaaccag acaccaatag accaaaaaac aaacattcaa
     2401 caagaaacat tttgaacaat atcacaaaac tgtcacaacc atcatcagac tattgaaaaa
     2461 aactggttgc tgttttataa aagcgactgt aacaacctcc actctatagt tccattagtt
     2521 taaagtgtca cacaaactga gcatccattt gttttcctta cttttgcccc tgtattccaa
     2581 ggggtttttt tataacttaa agattagcac accacacaaa aacaataaaa aaaaacgaca
     2641 agttatacaa taaaaccagt actatccata cagctaatta catataaata ctattaaacc
     2701 atctggacat taaaaataaa ctcttataac ataaaattaa tgcggttccg gtaccatatg
     2761 tcagggataa tgtgcaaatg gaggtatcgg aactgcattt cagttcatta ttaattaatg
     2821 acaaaaaaaa cctctgcgag cgcagagggg gtaaggaatg ttcccggtca taatatgtcg
     2881 atagtgttta tatacataca gaacaagaac aacaaccagc caactaataa atattttatt
     2941 ttttagagct gaaaccttaa agctcacttc cattccatac attgttataa accttatgtt
     3001 gaaaactgaa agtaagtacg gcagacttat taagttgcgt gcagtctttt cacgttatac
     3061 aaacaagaac acctgcgaag ttatttctcc ttacatatcc ccctatgcca acgggggatt
     3121 ttttctggtt cactaacgca taaataatag tgacacccta tcattcacaa accgtgataa
     3181 tataaggatt atcatcatgg caaaattagg tgaaaacacc ccacatatta tcgatgctac
     3241 tgtagatttt atggcatcaa gccaggcatt tcgtgagtat ctgaaaaaac ttccccctga
     3301 taacacaata ccgccagctc ttactgatga gcacgtaccg ttatatatac gaaggctgga
     3361 atactatcgt ctgctttatc gaccaaagca gacagaatca tattgaaaaa aacggtgaac
     3421 ggttcgcgaa atcctttcgc gggtcgtgag gcgttggaca agcgaagcgc gtcagttttt
     3481 ccggctcttc cccgtgacag gtttctccac cagtttatct tccagtgtca caactttctg
     3541 atacaccgcc gctgtggcgt tgtagttcct gtcgagtatt gttttcattc cccatccgag
     3601 cacacagaac ataaccagaa aagccactga caccccaccc agccatttcc agctataccc
     3661 tttaagggtg ttgtattcct gcacgatatt gctgtgaatt gtcgcaatat ccctgttcag
     3721 ggcatccgcg tttgccttca tgtttgcgac tgactcttcc agccgctgaa aatcctggct
     3781 gatgcgctca cctatcagtc ctgccacgtc cagcttctgc acctcatttc tgacttcatc
     3841 cctgacctgt tttgccacac cgtcagaaat atcccgatat ccgcgaagag tttcccttgc
     3901 cacagccaga gaatcagcaa cagctttctg ttgctccatt gcggtatgtt gcagatgctc
     3961 caccgataac aggagcgtga gtaatttttc ttctgtgctg gccatgttca gaatcccata
     4021 gaaaatccac gtgacggtga tttaacccgt tcaggtgcag ttcggttctg tctgctcact
     4081 ctgtctctca tgtcaaagcc atgctcccgc gcaacatcat gttgcagcct tctgaccagt
     4141 tcatgttgct taaccggagt gtccagccat tccctgatgt catgcccgac cgttcgcgcc
     4201 tgttcaagat ggcgggaata gtccggttca gaccgttttt gcatgatgtc atgacgctgg
     4261 gcatcatcaa gccgtccggc agcttcaaaa cccagatgca cttcgggacg tcgttcaatg
     4321 ccctgttctt tcagcgtccg gcagtcaatg aagccccttt caaagccatg ctttctcata
     4381 tgttcgttgt gcttcacctc ccagcgtttt cgcaattccc gcaactgctc tttttgctga
     4441 gtgagagtca ggcttccgga atcctttttc gcacctcccc gctcgggata cctggcgtta
     4501 tagcgccgga aatactgttc cggtgtacgt tcaatgccat cattcacacg ctgactcatc
     4561 atgatatgcg catgaggctg ttcgccgccg tcaatgctcg ctttcggatt gtgaatggca
     4621 aaactccagg cgtgccgttc gccagcttcc tgacgtacaa aatcctgcac cagttcaagg
     4681 cgctgttccg gtgtcagttc acggggtaat gcaatttcaa gttccctata ggtcgaaccg
     4741 ttcgcacgtt caaactggtc agccgcctgc cagaaatggg aaggattatc cctggcccat
     4801 tccggcatgt ttccgtgtgc ggaatattcc aaatcctgac ggtcgcggta tttctctttg
     4861 cgctcgatgt aatcggcgtg gtttgcagcc tgccccttgc caccaaattt aacgctgagg
     4921 tgataggttg ccacggtttc agccctctgc aggagcgcac tgtttctcga cgaaggagag
     4981 aaacatataa gtgcgcttcc ttctaatttt acgaattagc acggaagata ccccctgcac
     5041 ccgccgaccg caagcgatat actgcacgac cacaacacac cgcaaaaaaa caccagggag
     5101 catcacgaac atgcgcatca gcaacatcga atggctgaag aaacggatcg gattcatcag
     5161 gaagctc
//
