LOCUS       NZ_CP069852             8731 bp    DNA     circular CON 03-DEC-2023
DEFINITION  Acinetobacter baumannii strain FDAARGOS_1359 plasmid unnamed,
            complete sequence.
ACCESSION   NZ_CP069852
VERSION     NZ_CP069852.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN16357501
            Assembly: GCF_016903215.1
KEYWORDS    RefSeq.
SOURCE      Acinetobacter baumannii
  ORGANISM  Acinetobacter baumannii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Acinetobacter; Acinetobacter calcoaceticus/baumannii
            complex.
REFERENCE   1  (bases 1 to 8731)
  AUTHORS   Carlson,P., Fischbach,M., Hastie,J., Bilen,M., Cheng,A., Tallon,L.,
            Sadzewicz,L., Zhao,X., Boylan,J., Ott,S., Bowen,H., Vavikolanu,K.,
            Mehta,A., Aluvathingal,J., Nadendla,S., Yan,Y. and Sichtig,H.
  TITLE     FDA dAtabase for Regulatory Grade micrObial Sequences (FDA-ARGOS):
            Supporting development and validation of Infectious Disease Dx tests
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8731)
  AUTHORS   Carlson,P., Fischbach,M., Hastie,J., Bilen,M., Cheng,A., Tallon,L.,
            Sadzewicz,L., Zhao,X., Boylan,J., Ott,S., Bowen,H., Vavikolanu,K.,
            Mehta,A., Aluvathingal,J., Nadendla,S., Yan,Y. and Sichtig,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2021) Institute for Genome Sciences, University of
            Maryland School of Medicine, BioPark II, 801 W. Baltimore St., Suite
            619, Baltimore, MD 21201, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SMRT v. 8.0.0, HGAP v. 4
            Genome Coverage                   :: 829.45x
            Sequencing Technology             :: Pacbio; Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 12/03/2023 04:02:55
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,722
            CDSs (total)                      :: 3,626
            Genes (coding)                    :: 3,554
            CDSs (with protein)               :: 3,554
            Genes (RNA)                       :: 96
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 23 of 72
            Pseudo Genes (incomplete)         :: 39 of 72
            Pseudo Genes (internal stop)      :: 16 of 72
            Pseudo Genes (multiple problems)  :: 6 of 72
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP069852.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..8731
                     /organism="Acinetobacter baumannii"
                     /mol_type="genomic DNA"
                     /strain="FDAARGOS_1359"
                     /culture_collection="FDA:FDAARGOS_1359"
                     /db_xref="taxon:470"
                     /plasmid="unnamed"
                     /geo_loc_name="USA: MD"
                     /collected_by="FDA-Stanford Microbiome Effort"
     gene            complement(21..2432)
                     /gene="znuD2"
                     /locus_tag="I6K89_RS18475"
                     /old_locus_tag="I6K89_18475"
     CDS             complement(21..2432)
                     /gene="znuD2"
                     /locus_tag="I6K89_RS18475"
                     /old_locus_tag="I6K89_18475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000932945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor ZnuD2"
                     /protein_id="WP_000932953.1"
                     /translation="MLNKSKLFLALITLGASKILLAAEGPVTTLNTIVLTAQSDELGSE
                     LLGKSLNVSNQFIDTSKLKQRSTTLGDALGTELGIHSNQYGGGASTPIIRGQEGKRIKV
                     LQNNADVLDMSNMSPDHAVTVEPSLAKSIEIIRGASTLLYSSNSAAGVVNVIDYKIPTQ
                     MPQDGLEGNTTLRFNTGSNEKLTTAGVTVGLSPHVALRAEGLYRNAGNYKTPHYQSSSY
                     NSLEDLENQNSIYKNLKYLPESWAESRVGTLGLSWIDDNTYLGVSYTHRHDEYGLPAHS
                     HLYEGCGASAIGIDSRISGLKNYLLYYPQLMDEQDINYINPRPDCHQHNHIHETNFSHN
                     APYIDLNTRRYDVRGEFTQPFTGIDKIRTSLSYIDYFHNELEGDKITNFFKNTGKVGRI
                     ELSHQPLGELTGILGLQYLEQDNSALSPVHSQEGHTTYLDNQQLLNRNVTKNFSVFGLE
                     KYNWNDFTFELGARIEKQKVSMDYDIEKIKDSMKPWPNKYNSPYVEKNNKIRAQNLKSI
                     LEAVQPNKETAFSYAGTVHWRFAPNYILSLTGTHQERLPNAQEMYTHGMHLATNSFEIG
                     NRFLRKEKSNNLEISLAYKDDLLDYQISTYYYDFDNYIYLQTLNEVLGTTKVRDQHTLR
                     INHYSQSAANFYGLEGNIGYQFNSVYHGSLFGDYVKGRLTNLPDAVIAYDIWNREPTLA
                     PQKDRYTPRLPPARLGTRLKADFDESLKGEIEYYRVFKQDNISKFEQVTSGYNMLNMTL
                     AYKNKLSHTEYDLFFKANNLLDQKVYAHETFLPYIPQIGRNFSLGLNLNF"
                     /name="znuD2"
     gene            complement(2560..2874)
                     /locus_tag="I6K89_RS18480"
                     /old_locus_tag="I6K89_18480"
     CDS             complement(2560..2874)
                     /locus_tag="I6K89_RS18480"
                     /old_locus_tag="I6K89_18480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006582675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnA antitoxin family protein"
                     /protein_id="WP_001983304.1"
                     /translation="MSMVRYSHKELNEKFGEKQDAEIQRLLAKGTVPDDQLDLSDIPEI
                     TDWSNAVRQNQFYRPVKQQTSIRLDADVLAWFKAQGKGYQTRMNEILRDAMLKELKNHQ
                     "
                     /name="BrnA antitoxin family protein"
     gene            complement(2861..3148)
                     /locus_tag="I6K89_RS18485"
                     /old_locus_tag="I6K89_18485"
     CDS             complement(2861..3148)
                     /locus_tag="I6K89_RS18485"
                     /old_locus_tag="I6K89_18485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019383542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnT family toxin"
                     /protein_id="WP_000438826.1"
                     /translation="MEQYFEWDEAKNRKNQKKHDISFETASLVFEDPLRISIQDRHTNG
                     EERWQTIGRVKGVLMLLVAHTIFDEDDCEIIRIISARQVTKAERNKYEHG"
                     /name="BrnT family toxin"
     gene            3521..4039
                     /locus_tag="I6K89_RS18490"
                     /old_locus_tag="I6K89_18490"
     CDS             3521..4039
                     /locus_tag="I6K89_RS18490"
                     /old_locus_tag="I6K89_18490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447193.1"
                     /GO_function="GO:0005515 - protein binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="WP_000447193.1"
                     /translation="METFAEPVFSNDLLAKAENGDTSAQLELAEIYLYGHGVDSDENQA
                     EVWALKSAENGNVAAMFWLADGYVTYARLMEDDDKNDSLEHFQKAFKWFQKASENGHSE
                     SMVELADLYTRADSGIEVNINKAFELREKAAKLGNKKAMRSLSVMYRDGIGIPKNTDLA
                     QSWWDKSEN"
                     /name="tetratricopeptide repeat protein"
     gene            complement(4228..4371)
                     /locus_tag="I6K89_RS18495"
                     /old_locus_tag="I6K89_18495"
     CDS             complement(4228..4371)
                     /locus_tag="I6K89_RS18495"
                     /old_locus_tag="I6K89_18495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004786602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001125246.1"
                     /translation="MPKLKDIALGIIVAPLLIPIMLIASYQDKKALKKELDECQKEKDQ
                     AS"
                     /name="hypothetical protein"
     gene            complement(4391..4966)
                     /locus_tag="I6K89_RS18500"
                     /old_locus_tag="I6K89_18500"
     CDS             complement(4391..4966)
                     /locus_tag="I6K89_RS18500"
                     /old_locus_tag="I6K89_18500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005102333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid replication DNA-binding protein"
                     /protein_id="WP_001096616.1"
                     /translation="MAKLSLSEVSKKFHVDRSTIYRAVRNGRLSRSSDGQFDLAEVIRC
                     FGEPEQTSQKIESSKQEGDESTKKLIAHLENEVKKYQEREERLMQQIDRMQTLIELKSV
                     APATAAPHQDATACDTKMPQHATTQQDTDNKKNNELNIAENVAVPQQETTAYHTQTLQH
                     ATLQSVAVPQHKKRGLFGRVLNAVFDND"
                     /name="plasmid replication DNA-binding protein"
     gene            complement(4959..5909)
                     /gene="repM"
                     /locus_tag="I6K89_RS18505"
                     /old_locus_tag="I6K89_18505"
     CDS             complement(4959..5909)
                     /gene="repM"
                     /locus_tag="I6K89_RS18505"
                     /old_locus_tag="I6K89_18505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004786597.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein RepM"
                     /protein_id="WP_001205343.1"
                     /translation="MRDLVVKDNALINASYNLDLVEQRLILLAIVEARESGKGINANDP
                     LEVHADSYINQFGVHRNTAYQALKDACKDLFARQFSYQEKKANGNIRNVMSRWVSQIAY
                     NDNEATVDLIFAPAVVPFITRLEEQFTKYELQQVSSLSSAYAIRLYELLIQWRSTGKTP
                     TIELQEFRKKLGVLDNEYLRMAHLKERVLELSIKQINEHTDITVKYEQHKRGRSISGFS
                     FTFKQKKKDSPSIERDPNTLELFSKMTDAQRHMFANKLSELPEMGRYSQGTESYQQFAV
                     RIAEMLQDPAQFKELYPYLKKVGYMPSNKKDTVNG"
                     /name="repM"
     gene            6593..6847
                     /locus_tag="I6K89_RS18720"
     CDS             6593..6847
                     /locus_tag="I6K89_RS18720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001014301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001014303.1"
                     /translation="MNGQKIDFLRFSKQGNKYFFPCKTKHRPYVTLANFPIQDPAHAGI
                     FLSHTTHLTMTPLKALQDKDFSDFLGSQKRVQKNGLFLE"
                     /name="hypothetical protein"
     gene            7119..7490
                     /locus_tag="I6K89_RS18510"
                     /old_locus_tag="I6K89_18510"
     CDS             7119..7490
                     /locus_tag="I6K89_RS18510"
                     /old_locus_tag="I6K89_18510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000504218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000504218.1"
                     /translation="MGEKVKTYAVRLDPQVAEFYDQLANSEGIRTSKLFSKILTNDFQS
                     IAVKQQADRIEDLVNRLEKRIDDFEEDNNFSEKYYEDFSGLYFMMLWLLMKNGASKDEV
                     RMIQAKGYSYADDNYKRVK"
                     /name="hypothetical protein"
     gene            7490..7663
                     /locus_tag="I6K89_RS18515"
                     /old_locus_tag="I6K89_18515"
     CDS             7490..7663
                     /locus_tag="I6K89_RS18515"
                     /old_locus_tag="I6K89_18515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001282484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001282484.1"
                     /translation="MSDNIIQLSKPCTLCENRQNVQLFAGLMLCGSCQENIRLTNPSMF
                     SANDEIEQKAQN"
                     /name="hypothetical protein"
     gene            complement(7986..8447)
                     /locus_tag="I6K89_RS18520"
                     /old_locus_tag="I6K89_18520"
     CDS             complement(7986..8447)
                     /locus_tag="I6K89_RS18520"
                     /old_locus_tag="I6K89_18520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004781077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DIP1984 family protein"
                     /protein_id="WP_000761346.1"
                     /translation="MKLAEALLLRSDQQKKIISLKQRINANVLVQDGDQPSEDPNELLK
                     QVFSLIQEFQKLSYAIHETNALTKLNDGRSLLALLTLRDEFVEQHKTLTAAISNTSRES
                     DRYSTREIKWHKVIPVSSLQKQADDISLKLRDLNVLIQSTNWKIDLIEA"
                     /name="DIP1984 family protein"
     Typing          complement(4960..5909)
                     /name="rep_cluster_1172"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 agaagattac agtatcttat ctagaaatta agatttaagc ctaaactaaa atttcttcca
       61 atttggggaa tatatggcaa aaatgtttca tgggcataga ctttctgatc aagtaaatta
      121 ttcgctttaa agaataaatc atattctgtg tgagatagct tatttttata tgctaaagtc
      181 atattaagca tattataacc tgatgtaacc tgctcaaatt tagaaatatt atcttgttta
      241 aagactcgat aatattctat ttcaccttta agcgattcat caaaatcagc ttttagtcgt
      301 gtacccaaac gagcaggagg taatctagga gtgtaacgat ccttttgggg agctaaagtc
      361 ggttctctat tccagatatc gtatgcaatt acagcatcag gtaaatttgt taaacggcct
      421 ttcacataat ctccaaaaag agagccgtga taaacagaat taaattgata ccctatgttg
      481 ccctctaatc cataaaaatt agcagcagac tggctataat gattaatacg tagtgtatgc
      541 tgatctctaa cttttgtagt tcctaaaact tcattgagtg tctgtaaata tatatagtta
      601 tcaaaatcat aatagtaagt actgatttga taatcaagta aatcatcctt ataggccaga
      661 ctgatttcta aattattgga tttctctttt cttagaaagc ggttacctat ttcaaaagaa
      721 ttagtagcga gatgcatacc atgagtatac atttcttgtg cattaggtaa tctttcttga
      781 tgtgtacctg ttaatgataa gatataattt ggagcaaatc tccagtggac tgtacctgca
      841 taagaaaaag ctgtttcttt gttaggttga acagcttcta aaatagattt taaattttga
      901 gccctgattt tattattttt ttctacataa ggactgttat atttattagg ccaaggtttc
      961 attgaatctt taattttttc tatatcataa tccatactaa ctttctgctt ttctatacga
     1021 gcacctagtt cgaaagtaaa gtcgttccag ttatattttt ctaggccaaa tacactaaag
     1081 tttttagtga catttcgatt taatagctgc tgattatcta gataggtggt atggccttct
     1141 tgagaatgga ctggtgataa agcactatta tcctgttcca aatattgtag ccccaagata
     1201 ccggttaatt cccctaatgg ctgatgtgat aactctatgc ggcctacttt tcctgtattt
     1261 ttaaaaaagt tagtaatctt atctccctcg agttcattgt gaaaataatc tatataactc
     1321 aatgaggtac gaattttgtc aataccggta aatggttgag tgaattctcc acgcacatca
     1381 taacgtcgag tatttaaatc aatataagga gcattatgag agaaattagt ttcatgaata
     1441 tgattatgct gatgacaatc aggacgtgga tttatgtaat taatatcttg ctcatccatc
     1501 aattgaggat aatataaaag ataatttttt aatccactaa ttcgtgaatc gataccgatt
     1561 gcagaggcac cacaaccttc atataaatgg ctatgggctg gtaaaccata ttcatcatga
     1621 cgatgtgtat aagaaactcc taaataagta ttatcgtcta tccaggataa tccaagcgta
     1681 cctactctcg actctgccca gctttctggt aaatatttta aatttttata aatactgttt
     1741 tggttttcaa gatcttctaa agaattataa gagctagact ggtaatgagg agttttatag
     1801 ttaccagcat tacgatataa cccttctgcc cgtaaagcta catgtgggct taagccaact
     1861 gttacacctg ctgtggtcaa cttctcatta ctgccagtat taaaacgtaa tgtagtatta
     1921 ccctctaatc cgtcttgagg catttgggtt ggaattttat agtcaatgac attgactacc
     1981 cctgccgcac tatttgaact atacaaaagg gtacttgcac cacgtataat ttcaatagac
     2041 ttagctaaac ttggttcaac tgtgacagcg tgatctgggg acatattaga catatccaac
     2101 acgtcagcat tattctgtaa aactttaata cgttttcctt cctgcccacg aataatagga
     2161 gtagatgctc cacctccata ttgattagaa tgaattccta attccgtacc caaggcatcc
     2221 cctagagttg tcgaacgctg tttaagttta ctagtatcaa taaactgatt actaacattt
     2281 agacttttgc ctaacagctc agaccctaac tcatcagatt gtgcagttaa tactattgta
     2341 tttaaggtag taacaggccc ttcagctgcc aataaaattt tcgaggcccc taaagtaatt
     2401 aaagctaaaa atagtttact tttatttagc ataaaacaac caaatttaat gttatattat
     2461 aatataacac ttaaaaattt ataaaatcta ctatttaaat ttttgaatgg taatcccccc
     2521 taaaaatgaa gtcaaaaatg ggagcctaag ctcccattct tattgatgat tttttaattc
     2581 ttttagcata gcatctcgta aaatttcatt catacgtgtt tgataacctt tgccttgtgc
     2641 tttgaaccaa gcaagtacat cagcatctaa ccgaattgaa gtttgttgct ttactgggcg
     2701 gtaaaattga ttttggcgta cagcattgct ccagtctgta atttcaggaa tatctgatag
     2761 atctagctga tcatcaggaa ccgtgccttt agcaagcaag cgttgaattt cagcatcttg
     2821 cttctcacca aatttctcat tcagctcttt gtgtgagtat ctaaccatgc tcatatttgt
     2881 ttcgctccgc tttagtgact tgccttgcac taatgattcg gatgatttca cagtcatctt
     2941 catcaaaaat ggtgtgagcc accaataaca ttagtacgcc tttaactcgt ccaatggttt
     3001 gccaacgttc ttcaccattg gtatgtctgt cttggattga gatccgtaat ggatcttcaa
     3061 aaacaaggct tgcagtttcg aaagagatat catgcttttt ctgattcttt cgattcttag
     3121 cctcatccca ttcaaaatac tgttccataa aaaacattca aatttgtata tacaataatg
     3181 tatcacaaaa ttgtatcact caaccagatt cgtgtaattt ttagtcaaaa ttattggcaa
     3241 gaagtatcaa aactgctcat aaaaaagccg acttgtttca aagtcggctt tttgggcatt
     3301 ttgggatttt tctattttta ttaagtagtt gatatttata ttaatataca agtgtacttc
     3361 gtataatcgc cattatgtta aatagagata agacatactc taattttaga gttttctttt
     3421 aggtatgata taaaaatata caagcctaac agtttagttt aaaggttaaa aattgaataa
     3481 attaacaaat atttttatgg ctttaggagt ttgcctttca atggaaacat ttgcagaacc
     3541 tgttttttct aatgatttat tagcgaaagc agaaaatgga gatacttctg ctcagttaga
     3601 attagccgag atttatctat atggtcatgg tgttgattca gatgaaaatc aagctgaagt
     3661 ttgggctctt aaatcagcag aaaatggaaa tgtagcagca atgttttggt tagctgatgg
     3721 atatgttact tatgctagat taatggaaga tgatgacaaa aacgattctt tagaacattt
     3781 ccaaaaagct tttaagtggt ttcagaaagc ctcagaaaat ggccattctg aatcaatggt
     3841 tgagttagct gatctatata ctcgtgcaga tagcggaata gaagttaata ttaataaagc
     3901 ttttgaactt cgtgaaaagg ctgcaaagtt aggtaataag aaagcaatgc gaagtctttc
     3961 cgttatgtat cgtgatggta taggtattcc taaaaatact gatttagctc aaagttggtg
     4021 ggataagtct gaaaattagt tttaatattt cctaaaatta acataataca ccttatacga
     4081 aatgctgtat aattatcctt ataattcaac gctgtagttt ctagtttata gcccgagcat
     4141 ccctgttcgg gcttttttta tgacttttca cgttccacgc tattatttca tcaatgttcc
     4201 acacttttga ttagctcaat tgaataatca ggatgcctgg tctttttctt tttggcattc
     4261 atccagctct ttcttcagcg cctttttatc ctggtacgat gcaatcagca tgatcgggat
     4321 cagcagcgga gctacaataa ttcccagggc aatgtctttc agtttcggca tgatctttca
     4381 cccttattgc tcagtcatta tcaaagacgg cattcagcac acggccaaat aaaccacgtt
     4441 ttttgtgttg tggcactgcc acactttgca acgtggcatg ctgtagcgtt tgggtgtggt
     4501 atgccgtagt ttcttgctgt ggcactgcta cattttctgc aatatttaat tcattattct
     4561 ttttgttatc agtgtcttgt tgtgttgtcg catgctgtgg catcttggtg tcgcatgccg
     4621 tagcatcttg gtgtggtgct gctgtggcag gtgcaacact tttcagctca atgagtgttt
     4681 gcatacggtc aatttgttgc attaaccgtt cttcacgttc ttggtatttt ttgacttcat
     4741 tttctaaatg ggcaataagt ttttttgtag attcatcacc ttcttgctta gatgattcaa
     4801 ttttttgaga tgtttgctca ggttctccaa agcatcgtat gacctctgct agatcgaatt
     4861 gtccatcact ggagcgtgat aaacgtccat tacgtacagc tctgtaaatg gttgatctat
     4921 ccacatgaaa ttttttagat acttcactta gtgataactt agccatttac ggtgtccttt
     4981 ttatttgatg gcatgtatcc cacttttttt aggtatgggt atagctcttt gaattgagcg
     5041 ggatcttgta gcatctcagc aatacgtaca gcaaactgtt ggtagctttc tgttccttgt
     5101 gaatagcgac ccatttcagg gagttctgaa agtttatttg caaacatatg ccgttgagca
     5161 tcggtcatct ttgaaaaaag ctccaaagtg ttcggatctc tttctattga tgggctatcc
     5221 ttcttcttct gtttaaaggt aaaagaaaat cctgaaatag aacgtcctct tttatgctgt
     5281 tcatatttta cagttatatc cgtatgctca tttatttgtt taattgaaag ctctaaaaca
     5341 cgctctttta aatgagccat ccgtaaatat tcattatcaa gaacgcctaa cttctttcta
     5401 aattcttgta gttctatggt tggggtttta ccagtgcttc gccactgaat tagaagctca
     5461 tataagcgaa tggcataagc gctactaaga ctactaactt gctgtaattc atatttagta
     5521 aattgttctt ccagtcgggt tatgaaggga acaacagcag gtgcaaatat taagtctaca
     5581 gttgcttcat tgtcgttata agcaatttga gatacccaac gactcataac atttcggata
     5641 ttcccattag cttttttctc ttgatagcta aattgacgcg cgaataaatc cttacaagca
     5701 tcttttaagg cttgataagc tgtattacgg tgtacaccaa attgattgat ataactatct
     5761 gcatgaactt ctaatggatc atttgcatta atccctttcc cactttccct tgcttcaaca
     5821 atagccaata aaattaaacg ttgttctact aagtctaagt tatagcttgc gttgattaag
     5881 gcattgtcct ttacaactaa atctctcata gccactattt tttattgtgt ataagtacac
     5941 gttaatatta tttgtgtact aatgcaaggt aaacgtggac atattgcaag gtaaacgtgg
     6001 acatattgca aggtaaacgt ggacatattg caaggtaaac gtggacatat aagagctaaa
     6061 accctttatt cataaggctt tcaagccatt taaaagtatt taaaaacttt aaaataatta
     6121 aaagcccagg gaatgaaaaa ttgcccttgg ttttcgcttc gctcaaactc tattgaactt
     6181 cgctttcgct cagttcgttg gggcaatttt ttggttaata ctggcgtgac tttaaaaaaa
     6241 agcaaaagaa acttggaatt cgcttcgctc ataaaacttt tttactcgct acgctcgatc
     6301 caggagcaaa tttctgcacg aaacttacgc tttcttaggc taaagaggac aaaaaaatgt
     6361 ttttcagagg gtctaggcgc aaattttgat agttcgctcg tagacactcg ctctatgcat
     6421 ctatctaaag tcaaaatacc tctttatttc gctctcattt gcgttttttg agctgttttg
     6481 tgtttttcac tatgagatct atgttttcta ggtaaaggct cttaaatcgc atttgaacgc
     6541 gttctatgaa gcttttcata attaaagttt tgggttgaaa tttttaacct ggatgaatgg
     6601 acaaaaaatt gattttttac gtttttctaa acaaggaaat aaatactttt ttccatgcaa
     6661 aaccaagcac cggccctacg tcactttagc caattttcca atccaagatc cggcacatgc
     6721 cgggattttt ttgtcacaca ccacgcatct aacgatgaca cccctcaaag ccttacagga
     6781 taaggatttc agcgattttt tagggtctca gaaacgtgta cagaaaaatg gtcttttttt
     6841 agaataagtg attgaattat ctatttttta tgctgtgctc atggttgtac atcaaaacaa
     6901 cacttttttt agataagata ttgataaatt taagaaaaaa tatgtactca tgattgtaca
     6961 taaaaaaagc attatattta aataagtttt tgttttagaa taaattaatt ctgtacaaat
     7021 aagtttgtac gtaatctaat tattattttt tatttaatta gttgaattat atatatttaa
     7081 ttttgtacaa aatctaattt taataagagg tgtaatttat gggcgaaaaa gtcaaaactt
     7141 atgcagtcag gttagatcca caagtagcag aattctacga tcaattggct aattcagaag
     7201 gaatcagaac cagtaaatta ttcagtaaaa ttttgaccaa tgattttcaa tctattgctg
     7261 ttaaacagca agccgatcgt attgaggatc tggtgaatcg attagaaaag cgaatagatg
     7321 attttgagga agataacaat ttttctgaaa aatactatga agacttttct ggactatatt
     7381 tcatgatgtt gtggctctta atgaaaaatg gcgcttctaa agatgaggtt agaatgatac
     7441 aagctaaagg ttattcatat gcagatgata actataagag ggtaaagtag tgtcagataa
     7501 tattattcag ctttcaaagc catgtacgct ttgtgaaaat agacaaaatg tacaactttt
     7561 tgcaggattg atgctgtgtg gaagttgtca ggaaaatatc aggcttacga atccaagtat
     7621 gttctcggcc aatgatgaga ttgaacaaaa agcccagaat taacctgggc ttcgtataag
     7681 agattttatg ttaaatgccg aactgaatta aaaatagaaa tgtagttgag ctgatgcttg
     7741 ttcatttctt tatttttagt aattgaggtg aacatactgt cagtacatgg tagttataag
     7801 taacgtgtaa ctttgtttgc acaaagattt gagttatgcc aagtgatgga ttgatttgta
     7861 cttaaaattt taagcttatc cacttgcttt gcaagccact acactttatt tacaatccta
     7921 tgttgtggaa gtaagggatc ttgtactcgc ccagtatgtt caacttgatt atatctaaag
     7981 gactattagg cttcaatcaa atcaattttc caattagtcg actgaattaa cacgtttaaa
     8041 tcacgtaatt taagactaat atcatcagct tgtttttgta atgatgaaac tggaataact
     8101 ttatgccact ttatttcccg cgtactataa cgatcagatt cacgagatgt attacttatt
     8161 gctgcagtta gggttttgtg ctgttctaca aattcatcac gcaaagtcaa taaagcaagt
     8221 agagaacggc catcatttaa ttttgttaaa gcattagttt catgaattgc ataacttaat
     8281 ttttgaaatt cttgaattaa agaaaatact tgttttagta gttcatttgg atcttcagat
     8341 ggttgatcac catcttgtac caatacattt gcattgatac gttgttttag tgaaataatt
     8401 tttttttgtt gatcacttcg aagtaagaga gcttctgcta atttcatttt tcacctatta
     8461 accaattaga attaagtttt aaacagctta tatcctcatt ctttaaagat caaaaaaatt
     8521 gctttttatc tagctaaaag gtgtctacaa aatcggtggc tattcagtgc tgaaaaggct
     8581 gccgacacat aaagtgaccc agattgaaga gttactgcca cactgctgga aacctaaatc
     8641 gaattaaaaa ataggtatgg gattcagcgg acgcttacct taattattaa tctaatcatt
     8701 ttataagaaa aatcttctaa gaaattttct t
//
