LOCUS       NZ_CP069839             1908 bp    DNA     linear   CON 03-DEC-2023
DEFINITION  Klebsiella pneumoniae strain FDAARGOS_1327 plasmid unnamed10.
ACCESSION   NZ_CP069839
VERSION     NZ_CP069839.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN16357469
            Assembly: GCF_016903115.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 1908)
  AUTHORS   Sokurenko,E., Tallon,L., Sadzewicz,L., Zhao,X., Boylan,J., Ott,S.,
            Bowen,H., Vavikolanu,K., Mehta,A., Aluvathingal,J., Nadendla,S.,
            Yan,Y. and Sichtig,H.
  TITLE     FDA dAtabase for Regulatory Grade micrObial Sequences (FDA-ARGOS):
            Supporting development and validation of Infectious Disease Dx tests
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1908)
  AUTHORS   Sokurenko,E., Tallon,L., Sadzewicz,L., Zhao,X., Boylan,J., Ott,S.,
            Bowen,H., Vavikolanu,K., Mehta,A., Aluvathingal,J., Nadendla,S.,
            Yan,Y. and Sichtig,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2021) Institute for Genome Sciences, University of
            Maryland School of Medicine, BioPark II, 801 W. Baltimore St., Suite
            619, Baltimore, MD 21201, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SMRT v. 8.0.0, HGAP v. 4
            Genome Coverage                   :: 763.24x
            Sequencing Technology             :: Pacbio; Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 12/03/2023 04:05:30
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,467
            CDSs (total)                      :: 5,342
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 125
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 36 of 104
            Pseudo Genes (incomplete)         :: 63 of 104
            Pseudo Genes (internal stop)      :: 13 of 104
            Pseudo Genes (multiple problems)  :: 8 of 104
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP069839.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..1908
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="FDAARGOS_1327"
                     /host="Homo sapiens"
                     /culture_collection="FDA:FDAARGOS_1327"
                     /db_xref="taxon:573"
                     /plasmid="unnamed10"
                     /geo_loc_name="USA: WA"
                     /collected_by="ARMADA"
     gene            complement(279..1268)
                     /locus_tag="I6K57_RS27170"
                     /old_locus_tag="I6K57_27170"
     CDS             complement(279..1268)
                     /locus_tag="I6K57_RS27170"
                     /old_locus_tag="I6K57_27170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="WP_001529700.1"
                     /translation="MKNPELQNLTDYSPSDAPWDAHRSVSDDVGGIYLLAAEYERYGAR
                     MALCGGLLRFGWSTLKETGETRLRLREAHFCRVRHCPVCQWRRSLMWQARFYQSLPRIV
                     ADYPDARWMFLTLTVRNCAIGELGEILNRMNAAFQRLKDRKEFRPVQGWIRTTEVTRSS
                     DGSAHPHFHTLMMVPPSMLSGDGYVKHARWVELWRECLRVSYDPNVDVRAVKPRKPKDG
                     ESLACATAELVRGAVAETLKYSTKPADMVADPEWFLELTRQTHKRRFVATGGALKDVLK
                     LDQETDADMVIGDDISEGDDDGSRIAFEWKTEVRKYRRSPTKDKTESE"
                     /name="protein rep"
     Typing          362..553
                     /name="ColpVC"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="97.409"
                     /coverage="100.0"
                     /evalue="6.18e-88"
ORIGIN
        1 ggtatcgggg cctgccctga ccatgttgtt tttggatgcc gctgcgtgtg gcggtactcc
       61 aaaaacacat cttgtcccgt caaaaaaaat ccgtattacg gatcgcaaaa ccctaagttc
      121 ggattccgaa ctaggcaatc gagtgctagc actttgcggc gttagcccta tcctgcatcg
      181 gagtaaaacc aatcggacaa gatgtgactt tggtacaccg ccaccaaggt gacggatgcc
      241 caaagtcctc atggtcaggg cgcagccccg acaccccgtt actctgattc ggttttatct
      301 ttcgtcggtg agcgacggta ctttctgacc tcagttttcc actcgaacgc gatgcgggaa
      361 ccgtcatcgt cgccctcgga aatatcatcc ccgatcacca tgtctgcgtc ggtttcctga
      421 tcgagcttca gtacgtcttt gagtgcgccg ccagtcgcca caaagcggcg cttgtgcgtc
      481 tggcgggtca gttcgaggaa ccattcagga tcggccacca tgtcagccgg cttcgtggaa
      541 tacttcaggg tttcggccac cgcaccgcgc accagttcgg cggtcgcgca ggccagcgat
      601 tcgccgtcct tcggcttccg gggttttact gcccggacat caacattcgg gtcgtaactg
      661 acccgcaggc actcccgcca gagttcaacc cagcgagcat gttttacgta accgtcacca
      721 gatagcattg agggcggcac catcatcagg gtgtggaagt ggggatgtgc gctgccgtca
      781 ctgctgcggg tgacttccgt ggtacgtatc cagccctgca caggccggaa ctccttgcga
      841 tccttcagtc gctggaaagc tgcattcatc cggttcagta tctcgcccag ctccccgatc
      901 gcacagttgc gcacggtcag ggtcaggaac atccagcggg catcagggta atccgccacg
      961 atacgcggca gggactggta gaaacgggct tgccacatca gggagcgcct ccactggcag
     1021 acaggacagt gccggacacg gcagaaatgc gcctcacgca gcctcagacg cgtttctccg
     1081 gtttccttga gggtagacca cccgaaacgc aataaaccgc cacagagggc catacgcgcc
     1141 ccgtagcgct catattcggc cgcaagcagg taaatccctc ccacgtcgtc agaaacgctt
     1201 ctgtgagcgt cccagggcgc atctgagggg gaatagtccg tgaggttctg gagttcagga
     1261 tttttcatgc acaccacccg attgtgggcg atggctcatc tgacgtgtta tcattgccat
     1321 atcacctgtg ttagccgtat caccaattcg aatctctaca ggtggtcacg cccccgaacg
     1381 atgcccgtcg ttgcgggggt cgcttttaac gcgaccactt ccgacagaat acttctaaac
     1441 ccatttcgga gcaaatgtgc cctcaagcct tgcgggacaa gggctggagg ccaatttgaa
     1501 tcccagagtt atcccttaag tataaataca aggatcgcgc gcgagatttg ccacccgaaa
     1561 accgcgttaa atcaccccca aaaaaaccca aaaccgctta ccaggtggtt gcgctcactg
     1621 cgtgatcaca accacctgtc ggcagtgttt tacgggcgcg gtcgtcaatc actaaatcgg
     1681 aacccactgg caagccagcg ggaccccacg atttagcgtt tgacgatttc aggaacggcg
     1741 aacgctggca gatttcaatc tggcggcgtg aggtgttggc agggtgtcgg ggcgcagccc
     1801 tgacggatcg tttttggtac ccgcatcgtg atggcggtac tccaaaaaca catcttgtcc
     1861 cgtcaaaaaa aatccgtatt acggatcgca aaaccctaag ttcggatt
//
