LOCUS       NZ_CP069838             2422 bp    DNA     linear   CON 03-DEC-2023
DEFINITION  Klebsiella pneumoniae strain FDAARGOS_1327 plasmid unnamed9.
ACCESSION   NZ_CP069838
VERSION     NZ_CP069838.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN16357469
            Assembly: GCF_016903115.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 2422)
  AUTHORS   Sokurenko,E., Tallon,L., Sadzewicz,L., Zhao,X., Boylan,J., Ott,S.,
            Bowen,H., Vavikolanu,K., Mehta,A., Aluvathingal,J., Nadendla,S.,
            Yan,Y. and Sichtig,H.
  TITLE     FDA dAtabase for Regulatory Grade micrObial Sequences (FDA-ARGOS):
            Supporting development and validation of Infectious Disease Dx tests
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2422)
  AUTHORS   Sokurenko,E., Tallon,L., Sadzewicz,L., Zhao,X., Boylan,J., Ott,S.,
            Bowen,H., Vavikolanu,K., Mehta,A., Aluvathingal,J., Nadendla,S.,
            Yan,Y. and Sichtig,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2021) Institute for Genome Sciences, University of
            Maryland School of Medicine, BioPark II, 801 W. Baltimore St., Suite
            619, Baltimore, MD 21201, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SMRT v. 8.0.0, HGAP v. 4
            Genome Coverage                   :: 763.24x
            Sequencing Technology             :: Pacbio; Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 12/03/2023 04:05:30
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,467
            CDSs (total)                      :: 5,342
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 125
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 36 of 104
            Pseudo Genes (incomplete)         :: 63 of 104
            Pseudo Genes (internal stop)      :: 13 of 104
            Pseudo Genes (multiple problems)  :: 8 of 104
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP069838.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..2422
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="FDAARGOS_1327"
                     /host="Homo sapiens"
                     /culture_collection="FDA:FDAARGOS_1327"
                     /db_xref="taxon:573"
                     /plasmid="unnamed9"
                     /geo_loc_name="USA: WA"
                     /collected_by="ARMADA"
     gene            239..424
                     /locus_tag="I6K57_RS27160"
                     /old_locus_tag="I6K57_27160"
     CDS             239..424
                     /locus_tag="I6K57_RS27160"
                     /old_locus_tag="I6K57_27160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin VbhA family protein"
                     /protein_id="WP_204724674.1"
                     /translation="MTKQQRIDGFRKAEASLKLEGMDPSGTPFYESVKARIISGEITYE
                     QGKAEILSHHKARAEG"
                     /name="antitoxin VbhA family protein"
     gene            1302..2297
                     /locus_tag="I6K57_RS27165"
                     /old_locus_tag="I6K57_27165"
     CDS             1302..2297
                     /locus_tag="I6K57_RS27165"
                     /old_locus_tag="I6K57_27165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020240588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="WP_204724673.1"
                     /translation="MQNPKLRNLTDYSPDDKPWDAHKSQSDDVGGIYLRAAEFESYAAR
                     MRDCGGLLRFGWSTLKDTGETRLRLREAHFCRVRHCPVCQWRRSLMWQARFYQSLPKIV
                     ADYPDGRWMFLTLTVRNCDVAELGETLTAMNAAFQRMKDRKEFKSVQGWIRTTEVTRGR
                     DGSAHPHFHTLMMVPPSMLSGRDYVKQYRWVELWRDCLRVDYDPNVDVRAVKPRKPKDG
                     QTLACATAELVRGAVAETLKYSTKPADMVADPEWFLELTRQTHKRRFVATGGALKDVLK
                     LEQETDQDMVIGDDISDGEDDGSRLAFGWDSTPRKYVRLPERDKFKSDSD"
                     /name="protein rep"
     Typing          complement(2018..2208)
                     /name="ColpVC"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="84.375"
                     /coverage="98.0"
                     /evalue="1.4200000000000001e-44"
ORIGIN
        1 gtgtcggggc gcagccctga cggatcgttt ttggtacccg catcgtgatg gcggtactcc
       61 aaaaacacat cttgtccggt acacagattt cctttggcat tccttcgggt ttccctgaga
      121 tttccactgt gcgtgcatac acgtatacac gcataaccaa cggcacagtt cgggcaggac
      181 atgtaaagtt agccaacttt acgcctgcct tattcgcatt ctgctcaaag gaaacctcat
      241 gacaaaacag caaaggatcg acggattccg taaagcggaa gcatcgttaa aactggaggg
      301 tatggatcct tctggaactc ccttttatga gtcggtaaaa gcccgaatca tctccggtga
      361 aatcacctac gaacagggca aagctgagat tctttcccac cacaaagccc gcgcagaagg
      421 ttgaatcgtc aacttctaaa tcgggggatc cctttagggt tccgatttag tggttgtacg
      481 actgcacccc tcataccctt gtgatgggtg atggttcacg tagtgagcca tcgccctgta
      541 aagccgcttt ttaccccctt caaggcgttg cctgtggggt aggtgcgtaa gccctgccct
      601 acaggtgatg ccgtgggggt attttcgttt tgctcggtat gccttattcg tttaaatccg
      661 ccatcaggat tatgcggggt aaagtcgtgc ggccgcgatg aggaacgggg attgcccccg
      721 cagctctgcg aggaggcaca cgccgttcgg aatcggggtc cactctgttt aatagtggac
      781 tcttgttgcg atttgcaacg gaaacagccg cgtgcagtga atgtaatact gcacgtttgt
      841 ctcaatctga catttctcag ggggggcgat ttaacgtggt ttgcttaatt tttgagcgtg
      901 cgaatgcttg agtgtttgag tgcctgaatg ctgcttttgg gggttggttt ttcgaccctg
      961 aaaggggcga aatccttgta tttatactta agggataact ctgggattaa aattcgcttc
     1021 catcccttgt gccgcaaggc ctgagagcca atttgctcgg aaaaggtttt ggcagtatga
     1081 tttctgacag gtgatcgtgt ccgcgaacta atttttgatt acttggggtg gttcccaaga
     1141 ccttacttac tgacacgatg atatgagaaa cccctgcaag tgttaccagc acttcagggg
     1201 cgcgatcacc tggctaaata cggtttcaaa tcgactctca acaggtgata cggaaatgat
     1261 aacacaccct tccagtgcct gcccatattg gagcaggtgc tatgcaaaac cctaagctaa
     1321 gaaacctcac tgactacagc cccgacgata agccgtggga tgctcataag tcacaatctg
     1381 atgatgttgg cggtatctat ctgcgtgctg ctgaatttga gtcgtatgca gcccgtatgc
     1441 gcgattgtgg cggtctcctg cgttttggct ggtctaccct taaagacacc ggagaaacgc
     1501 gtttacggct ccgtgaggcg catttctgcc gcgttcgtca ctgccctgtc tgccagtgga
     1561 ggcgctccct gatgtggcaa gcccgttttt atcagtctct gccgaaaatc gtcgctgatt
     1621 accctgatgg ccgatggatg tttctgacgc tcaccgttcg caactgcgat gttgctgaac
     1681 tcggcgaaac gcttactgcc atgaatgccg catttcagcg catgaaggat cgcaaggagt
     1741 ttaaatccgt acagggatgg attcgcacca cagaagttac ccgcggtcgt gatggctctg
     1801 cacaccccca ctttcacacc ctgatgatgg ttcctccctc gatgctttcc ggtcgtgatt
     1861 acgtaaaaca atatcgctgg gttgaactct ggcgcgactg cctgcgtgtt gattacgatc
     1921 cgaatgttga tgttcgggca gtgaaacccc gtaagcctaa agatggccag acgctggcat
     1981 gtgcaactgc cgaactggtg cgcggtgccg ttgctgaaac tctgaaatat tcgacaaagc
     2041 cggctgatat ggttgctgac ccggaatggt tcctggagct gacccggcag acgcataaac
     2101 gccgttttgt tgcgaccggt ggcgcgctga aagacgttct gaaactggag caggaaacgg
     2161 atcaggacat ggtgatcggg gatgatattt ccgatggtga agacgatggt tcacgcctcg
     2221 cctttggctg ggattcgacg ccaagaaaat acgtccgttt gcctgagcgc gataagttta
     2281 aatcagattc tgattagggt atcggggcct gccctgacca tgttgttttt ggatgccgct
     2341 gcgtgtggcg gtactccaaa aacacatctt gtccggtaca cagatttcct ttggcattcc
     2401 ttcgggtttc cctgagattt cc
//
