LOCUS       NZ_CP069836             2579 bp    DNA     circular CON 03-DEC-2023
DEFINITION  Klebsiella pneumoniae strain FDAARGOS_1327 plasmid unnamed8,
            complete sequence.
ACCESSION   NZ_CP069836
VERSION     NZ_CP069836.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN16357469
            Assembly: GCF_016903115.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 2579)
  AUTHORS   Sokurenko,E., Tallon,L., Sadzewicz,L., Zhao,X., Boylan,J., Ott,S.,
            Bowen,H., Vavikolanu,K., Mehta,A., Aluvathingal,J., Nadendla,S.,
            Yan,Y. and Sichtig,H.
  TITLE     FDA dAtabase for Regulatory Grade micrObial Sequences (FDA-ARGOS):
            Supporting development and validation of Infectious Disease Dx tests
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2579)
  AUTHORS   Sokurenko,E., Tallon,L., Sadzewicz,L., Zhao,X., Boylan,J., Ott,S.,
            Bowen,H., Vavikolanu,K., Mehta,A., Aluvathingal,J., Nadendla,S.,
            Yan,Y. and Sichtig,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2021) Institute for Genome Sciences, University of
            Maryland School of Medicine, BioPark II, 801 W. Baltimore St., Suite
            619, Baltimore, MD 21201, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SMRT v. 8.0.0, HGAP v. 4
            Genome Coverage                   :: 763.24x
            Sequencing Technology             :: Pacbio; Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 12/03/2023 04:05:30
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,467
            CDSs (total)                      :: 5,342
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 125
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 36 of 104
            Pseudo Genes (incomplete)         :: 63 of 104
            Pseudo Genes (internal stop)      :: 13 of 104
            Pseudo Genes (multiple problems)  :: 8 of 104
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP069836.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2579
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="FDAARGOS_1327"
                     /host="Homo sapiens"
                     /culture_collection="FDA:FDAARGOS_1327"
                     /db_xref="taxon:573"
                     /plasmid="unnamed8"
                     /geo_loc_name="USA: WA"
                     /collected_by="ARMADA"
     gene            join(2496..2579,1..561)
                     /locus_tag="I6K57_RS27140"
                     /old_locus_tag="I6K57_27140"
     CDS             join(2496..2579,1..561)
                     /locus_tag="I6K57_RS27140"
                     /old_locus_tag="I6K57_27140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017900990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolone resistance pentapeptide repeat protein
                     QnrB19"
                     /protein_id="WP_012954666.1"
                     /translation="MTLALVGEKIDRNRFTGEKVENSTFFNCDFSGADLSGTEFIGCQF
                     YDRESQKGCNFSRAMLKDAIFKSCDLSMADFRNVSALGIEIRHCRAQGADFRGASFMNM
                     ITTRTWFCSAYITNTNLSYANFSKVVLEKCELWENRWMGTQVLGATFSGSDLSGGEFST
                     FDWRAANFTHCDLTNSELGDLDIRGVDLQGVKLDSYQASLLMERLGIAVIG"
                     /name="quinolone resistance pentapeptide repeat protein
                     QnrB19"
     gene            complement(943..1179)
                     /locus_tag="I6K57_RS27145"
                     /old_locus_tag="I6K57_27145"
     CDS             complement(943..1179)
                     /locus_tag="I6K57_RS27145"
                     /old_locus_tag="I6K57_27145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001676031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_012772745.1"
                     /translation="MARLTNLTPAEKKFLDDAVAAAERASGKKLNQPNRHIVLNRARAQ
                     IELQRYADRQRALREDERQQSDFAWSRPRAPRR"
                     /name="hypothetical protein"
     gene            <2247..2402
                     /locus_tag="I6K57_RS27150"
                     /old_locus_tag="I6K57_27150"
                     /pseudo=""
     CDS             <2247..2402
                     /locus_tag="I6K57_RS27150"
                     /old_locus_tag="I6K57_27150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707212.3"
                     /GO_function="GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /name="helix-turn-helix domain-containing protein"
     ARG             2461..2579
                     /name="QnrB10"
                     /gene="QnrB10"
                     /gene_name="quinolone resistance protein (qnr)"
                     /tool="rgi"
                     /drug_class="fluoroquinolone antibiotic"
                     /identity="97.5"
                     /coverage="17.7"
     ARG             2..561
                     /name="QnrB5"
                     /gene="QnrB5"
                     /gene_name="quinolone resistance protein (qnr)"
                     /tool="rgi"
                     /drug_class="fluoroquinolone antibiotic"
                     /identity="99.46"
                     /coverage="82.3"
     Typing          complement(1345..1518)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="2.5600000000000002e-86"
     Typing          1497..1988
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="92.51"
                     /coverage="81.0"
                     /evalue="0.0"
ORIGIN
        1 gatttttcag gtgccgacct gagcggcact gaatttatcg gctgccagtt ctatgatcgc
       61 gaaagtcaga aagggtgcaa ttttagtcgc gcaatgctga aagatgccat tttcaaaagc
      121 tgtgatttat caatggcaga tttccgcaac gtcagtgcct tgggcattga aattcgccac
      181 tgccgcgcac aaggcgcaga tttccgcggt gcaagcttta tgaatatgat caccacgcgc
      241 acctggtttt gcagcgcata tatcactaat actaatctaa gctacgccaa tttttcgaaa
      301 gtcgtgttgg aaaagtgtga gctgtgggaa aaccgctgga tggggactca ggtactgggt
      361 gcgacgttca gtggttcaga tctctccggc ggcgagtttt cgactttcga ctggcgagca
      421 gcaaacttca cacattgcga tctgaccaat tcggagttag gtgacttaga tattcggggt
      481 gttgatttac aaggcgttaa gttagacagc taccaggcat cgttgctcat ggagcggctt
      541 ggcatcgctg tgattggtta gttcagcgtt tacaccggga gatgcgtttg acgatttact
      601 tttacccgcg atcatcgcgt ttattgagtt tattatttat ctatgcgatt ccgatgaaaa
      661 attcgcacga aaatatggct aactcatagc aaaaaaaaag ccccctaagg gctttttttt
      721 gtacctgacg gtcagaccga ttaaacataa catcccttgc ccgcaccccc gcaggcgccc
      781 tcagcgcaat cctgctgcat cagggccgtt tccagcacct ttcctcacca gtatcccgga
      841 aaaacgcccc tgatgatttc tgcgcgactg catggctatg cacgcaggtg aatggcaggt
      901 gatttcgcag cgtcagcacc actaaaccgc caaaagccaa aatcagcggc gcggcgcgcg
      961 cggacgtgac caggcaaaat cggactgctg ccgctcatcc tcacgtaatg cacgctggcg
     1021 gtcggcgtaa cgctgcaact caatttgcgc acgcgctctg ttcaggacga tgtgccggtt
     1081 aggctgattt agctttttac ctgacgctct ttcagctgcg gcaacagcat catcaagaaa
     1141 ttttttctct gcaggcgtca agtttgtcag tctggccata acgttctcca gtcagaataa
     1201 atcagcacag tctgccagac acagtagaaa cgatgaagcg ccagcgacgg tgaaaggcag
     1261 tcagaaacgg tgaacgggag cggattttaa tccggtgccg tgaggcgttg gacaagccgc
     1321 aggcgcgtca ttagctttta gccggtttcg gggcgcagcc ctgaaccagt cacgtagcgc
     1381 tagcggagtg tatactggct gactatgttg gcacggatgt ggaaatcagt gaagtgcttc
     1441 atgtagcagg agaaaaaagg cggcaccagc gcgtcagcag gatatgtagt acaggatata
     1501 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtgcc
     1561 ggcttacagg cggggcggaa atttcctggg agataccagg aaggagctta acagggaaga
     1621 gataaggccg cggcgaagcc gtttttccat aggctccgcc cccctgacaa acatcatgaa
     1681 atctgacgct caaatcagtg gtggcgaaac ccgatgcggc ttaaagatac caggcgtttc
     1741 cccctggtgg ctccctcgtg ctcccccgca ttcctgcctt tcggtttacc ggtgtcattc
     1801 cgctgttatg gccgcgttta tctcattcca cgcctgacac tcagttccgc gaaggcagtt
     1861 cgctcctgga tggactgtat gcacgaaccc cccgttcagt ccgactgctg cgccttatcc
     1921 ggtaactatc atcttgagtc caacccggaa agacacgact tatcgccact ggcagcagcc
     1981 actggtaact gatttagagg agttagtctt gaagttatgc gcctgttaag gctaaactga
     2041 aagaacagat ttggttactg cgctcctcgt aagccagtta ccacggttca aagagttgct
     2101 gctctgagaa ccttcgaaaa accgccttgc agggcggttt tttcgtttac agagcaagag
     2161 attacgacgc cacagaaagg atctcaagaa gatccttcac cgtaatggaa atctatccaa
     2221 tccgagcaca taggatacac atcgttataa cgctggatct gcgtgaattc cagcagcagc
     2281 aagagaaaga ctttctacaa accagcttac agcaggctaa atttaaccag aaaaaagccg
     2341 ctgaattact gggtctgact tatcatcagc tacgtgcctt gctgaagaaa caccagatct
     2401 aatacgcatt tgcaggtgtc atactggcgg gtttgacgca taacgtcata aggtttacca
     2461 tggcgtcatt actgtataaa aacacaggca tagatatgac tctggcatta gttggcgaaa
     2521 aaattgacag aaatcgcttc accggtgaga aagttgaaaa tagtacattt tttaactgc
//
