LOCUS       NZ_CP069835             4981 bp    DNA     circular CON 03-DEC-2023
DEFINITION  Klebsiella pneumoniae strain FDAARGOS_1327 plasmid unnamed5,
            complete sequence.
ACCESSION   NZ_CP069835
VERSION     NZ_CP069835.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN16357469
            Assembly: GCF_016903115.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 4981)
  AUTHORS   Sokurenko,E., Tallon,L., Sadzewicz,L., Zhao,X., Boylan,J., Ott,S.,
            Bowen,H., Vavikolanu,K., Mehta,A., Aluvathingal,J., Nadendla,S.,
            Yan,Y. and Sichtig,H.
  TITLE     FDA dAtabase for Regulatory Grade micrObial Sequences (FDA-ARGOS):
            Supporting development and validation of Infectious Disease Dx tests
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4981)
  AUTHORS   Sokurenko,E., Tallon,L., Sadzewicz,L., Zhao,X., Boylan,J., Ott,S.,
            Bowen,H., Vavikolanu,K., Mehta,A., Aluvathingal,J., Nadendla,S.,
            Yan,Y. and Sichtig,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2021) Institute for Genome Sciences, University of
            Maryland School of Medicine, BioPark II, 801 W. Baltimore St., Suite
            619, Baltimore, MD 21201, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SMRT v. 8.0.0, HGAP v. 4
            Genome Coverage                   :: 763.24x
            Sequencing Technology             :: Pacbio; Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 12/03/2023 04:05:30
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,467
            CDSs (total)                      :: 5,342
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 125
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 36 of 104
            Pseudo Genes (incomplete)         :: 63 of 104
            Pseudo Genes (internal stop)      :: 13 of 104
            Pseudo Genes (multiple problems)  :: 8 of 104
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP069835.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4981
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="FDAARGOS_1327"
                     /host="Homo sapiens"
                     /culture_collection="FDA:FDAARGOS_1327"
                     /db_xref="taxon:573"
                     /plasmid="unnamed5"
                     /geo_loc_name="USA: WA"
                     /collected_by="ARMADA"
     gene            join(3885..4981,1..79)
                     /locus_tag="I6K57_RS27110"
                     /old_locus_tag="I6K57_27110"
     CDS             join(3885..4981,1..79)
                     /locus_tag="I6K57_RS27110"
                     /old_locus_tag="I6K57_27110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011217640.1"
                     /GO_function="GO:0005525 - GTP binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="WP_000893405.1"
                     /translation="MLEQVKDFLGDSYNPQTGEFNAEKARAEAEEHKDKFNIILCGATG
                     VGKSSLVNAVFGEEIVKSGVGSPVTQHLEKITVPRKGLVLWDTKGIESKDYENTINQLK
                     NDIFHAFNDSSHEDDVPHLGWVCIDSSGARIEERDIEIIGILREKEIPVVVVFTKILGD
                     ESADFIEAAISEINAHHGDFINGRYVKVNSRERKILNISIPVSGLDDLVDISFKALPES
                     KSRARQALKKAQMVKMEERLEAMKDSAKLIVHTSSVAAGVVGATPVPGSDAPLIAGVQS
                     TMIYKINSEFELDITTSASASIISGILGVTAVAQVGKTIVSNVLKFIPGVGSVVGGAIS
                     AATAVGLTEAIGHAYIQVLASYYDKTTGKVLLPESVSVVLDLFKKYFTYKK"
                     /name="GTPase"
     gene            complement(399..569)
                     /locus_tag="I6K57_RS27580"
     CDS             complement(399..569)
                     /locus_tag="I6K57_RS27580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_162708437.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPERHDLSPLAAATGNWIRRALMF
                     NQYYRVLEVMA"
                     /name="replication initiation protein"
     gene            complement(1228..1419)
                     /locus_tag="I6K57_RS27115"
                     /old_locus_tag="I6K57_27115"
     CDS             complement(1228..1419)
                     /locus_tag="I6K57_RS27115"
                     /old_locus_tag="I6K57_27115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165987.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGETL"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            1484..1807
                     /locus_tag="I6K57_RS27120"
                     /old_locus_tag="I6K57_27120"
     CDS             1484..1807
                     /locus_tag="I6K57_RS27120"
                     /old_locus_tag="I6K57_27120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_000955999.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGKLPTL
                     APPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVVAALMAIGDELRRLRLAVREQGAR
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            2016..>2447
                     /locus_tag="I6K57_RS27490"
                     /pseudo=""
     CDS             2016..>2447
                     /locus_tag="I6K57_RS27490"
                     /inference="COORDINATES: protein motif:HMM:NF015397.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            2485..3003
                     /locus_tag="I6K57_RS27130"
                     /old_locus_tag="I6K57_27130"
     CDS             2485..3003
                     /locus_tag="I6K57_RS27130"
                     /old_locus_tag="I6K57_27130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001447511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_204724669.1"
                     /translation="MSNLLRTGAEFEKKLKERAESTEKMLNDEFSRLGESVSEAVTSNE
                     TKIKGAIALFTTSTEESLKKHREGVKEAMMQHRKDVLKLAGNTGVMLLGIVFLLFTASG
                     GTLWYLGGRIQTNLEEIRVQEETLRKLNAKTWGVEFVQGGGRKFLVIPQGMSAEVTHYQ
                     GKDWIQLTE"
                     /name="MbeB family mobilization protein"
     gene            3010..3243
                     /locus_tag="I6K57_RS27135"
                     /old_locus_tag="I6K57_27135"
     CDS             3010..3243
                     /locus_tag="I6K57_RS27135"
                     /old_locus_tag="I6K57_27135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_001360356.1"
                     /translation="MTELETHLLNALEQLQQDYMQRLNEWESAFVELQKMFSLTQRDNA
                     MLNERVMQLSQQVQHLSGQTERLSQLYSENWK"
                     /name="MbeD family mobilization/exclusion protein"
     Typing          1071..1150
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="94.0"
                     /evalue="1.83e-34"
     Typing          complement(498..1042)
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.333"
                     /coverage="98.0"
                     /evalue="0.0"
     Typing          1798..3341
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="96.505"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 gactgggaaa gtcctccttc cagagagtgt tagtgtagtt ttggatttgt ttaagaaata
       61 cttcacatat aaaaagtaat ttatgggtga atatgtttta ccactctggt gataaatata
      121 ttcttaatac ggcctccagt tatggattta gttgatacaa cttaaacgaa gttatgtgag
      181 atagaggatt gcttcgtttg tatttgcatc aagttggcat agatgaaaag gatctccttg
      241 agatcctttg aaaggcgtcg taatctcttc cgctgtaaac gaaaaaaccg cctggggagg
      301 cggtttttcg aaggttaagt cagttgggga ctgcttaacc gggtaactgg cttcagcaga
      361 gcacagatac caaaactgtc cttccagtgt agccgtagtt aggccatcac ttcaagaact
      421 ctgtagtact gattaaacat caatgctctg cgaatccagt taccagtggc tgctgccagt
      481 ggcgataagt cgtgtctttc cgggttggac tcaagacgat agttaccgga taaggcgcag
      541 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
      601 gaactgagat accaacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag
      661 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
      721 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccgcctctg acttgagcgt
      781 cgatttttat gatgctcgtc aggggggcgg agcctatgga aaaacgccaa cggcgcagcc
      841 ttttccgggt tctcgttttt tgctcacatg tttcttttgg cgttattccc tgattctgtg
      901 gataaccgca tctccgcttt tgagtgagca gacaccgctc gccgcagccg aacgaccgag
      961 cgtagcgagt cagtgagcga ggaagcggaa gagcgccgga acgtgcattt tctccttacg
     1021 catctgtgcg gcatttcaca ccggacatgg tgcgctttcc atacaattcg cactgatgcc
     1081 gcatagttaa gccagtatac actccgctat cgctacgtga ctggttcagg gcttcgcccc
     1141 gaaaccccct gacgcgccct gacgggcttg tctgctcccg gcatccactc acaggcaagc
     1201 tgtgagcgtc tccgggagct gcttgtgtca gagggtttca ccgtcatccc cgaagcgtgc
     1261 gaggcagctg cggtaaagct catcggcgtg gtcgtgaaga gattcacaga tgtctgcctg
     1321 ttcatccgcg tccagttcgt tgagcttctc cagcagcgtt aatgtctggc ttctgataaa
     1381 gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg
     1441 atttctgttc atgggggtaa tgataccgat gaaacgcgag aggatgctca cgatacgggt
     1501 tactgatgat gaacatgccc ggttactgga acgctgtgag ggtaagcaac tggcagtatg
     1561 gatgcggcgg gtctgcctgg gtgagcctgt cgcccgttcc gggaagctgc cgacactggc
     1621 gccgccgtta ctgcgtcaac tggccgccat cgggaataac ctgaatcaga cagcgaggaa
     1681 ggtgaacagc gggcagtggt cttccggcga ccgggttcag gtggtggccg cactgatggc
     1741 catcggggat gagctgcgcc ggctgcgtct ggccgtcagg gaacaggggg cgcgggatga
     1801 tagttaaatt tcatgccagg ggaaaaggtg gtggcagtgg tccggttgat tacctgctgg
     1861 ggagggagcg taatcgcgaa ggcgcaacgg tgcttcaggg taatccggaa gaagtccggg
     1921 aactcatcga tgccacgcca tttgcgaaga aatacacgtc cggtgttctg tcgtttgcgg
     1981 agaaagaact gccaccggga ggacgtgaaa aagtgatggc gagttttgag cgtgtactga
     2041 tgcccggtct cgaaaaaaat cagtacagca tcctgtgggt ggagcaccag gacaagggac
     2101 ggcttgagct gaattttgtc attccgaaca tggagctaca gaccggaaaa cgcctccagc
     2161 cgtactacga ccgggcagac aggccgagaa ttgatgcctg gcagacactg gtgaatcacc
     2221 actacgggct gcatgacccc aacgccccgg agaatcgccg gacgctgaca ctccctgata
     2281 acctgccgga aacgaaacag gcgcttgctg agagcgttac acgaggcctg gaggtgcttt
     2341 accattccgg agagataaaa ggccgtcagg atgtgattca ggcgctcaca gaggtggggc
     2401 ttgaagtggt cagggtgacg cgaagcagta tcagcattgc agacccgaac ggcgggagga
     2461 atatcagact gaaaggagcg ttttatgagc aatcttttac ggacgggcgc ggagttcgag
     2521 aaaaagctga aagagagagc cgaatctaca gagaaaatgc tgaacgacga attcagcagg
     2581 ctcggcgaat ctgtaagcga ggctgtgacc tcaaacgaga cgaaaatcaa aggcgctata
     2641 gccctgttca caacgtcgac agaggaatcg ctgaaaaaac accgggaagg ggtgaaagag
     2701 gcgatgatgc agcaccggaa ggacgtgtta aagctggcag ggaatacggg cgtgatgtta
     2761 ctggggatag tctttctcct gtttaccgcg agtggcggga cgctctggta tctcggcggg
     2821 aggatacaga cgaacctgga agaaatcagg gtacaggaag agacattgcg gaaactgaac
     2881 gcgaagacat ggggcgtgga gtttgttcag ggcgggggaa gaaaatttct ggtcattccg
     2941 caagggatgt cagcagaggt gacgcactat caggggaagg actggataca actgacggag
     3001 tgatacggaa tgacagagct ggaaacacat ttgctgaacg ccttagagca gctgcaacag
     3061 gactatatgc agcggctgaa cgaatgggag agcgccttcg tggaattgca gaagatgttt
     3121 tcgcttacgc aacgggacaa cgcgatgctg aacgagcggg tcatgcagtt gagtcagcag
     3181 gtgcagcact tgagcgggca gacagaacgc ttgagccagt tatacagcga gaactggaaa
     3241 taagggagga acggctgata caggagcgcg aacatgcgtt atccctggaa cgggaacgcc
     3301 agctggaaat acaggaacgc acactggatg gtccttcgtt gtcgctgtga tggcgaaact
     3361 ataaaaaatg gcaggttcag tggattttga cggactaatg tagtctgcac catctggttg
     3421 cataggtatt catgcggtta aaatttatca ggcgcgatcg cggcagtttt tcgggtggtt
     3481 tgttgccatt tttacctgtc tgctgccgtg atcgcgctga acgcgtttaa gcggtgcgca
     3541 taaaatgggg ttatgttaaa tatgattgat atctatagat atcagactaa ctatttattt
     3601 tgtacatggt gttaaaattg gtaatgatta gaatggctat gacttataaa tgtttatcat
     3661 gccattggtg taaaactatt cctaaatgta gtgatgcttt agttgatgga gtgagcgcct
     3721 ttagtcggtg cccaaattgc gattctattt tggttgatga aaaaagagcc aacataatta
     3781 actcggttat tgcaaatata atattcaaaa taaaatcgta aatgatttta ttatttgtgt
     3841 gatcgtttgc gtttttgttg gtttgttatt atgtggaggt taaaatgttg gagcaagtta
     3901 aagatttttt aggtgattcc tataatccac aaactggtga gtttaatgca gaaaaggcaa
     3961 gggcagaggc tgaggaacat aaggacaaat ttaatataat tctttgtgga gctacaggag
     4021 ttggtaaaag ttctttggtt aatgcagttt ttggcgagga gattgttaag tcaggagtgg
     4081 gaagtccagt tacccagcat ttagaaaaaa tcactgttcc aagaaaaggg ctggtgttgt
     4141 gggatacaaa aggtattgag tcaaaagatt atgaaaatac cataaatcag ttaaagaatg
     4201 atatttttca tgcattcaat gactcatctc atgaagacga tgtgcctcat ttagggtggg
     4261 tttgtattga ttcgtcaggt gcaaggatag aagaaagaga tattgagata attggtattt
     4321 taagagaaaa agaaatacct gtagttgttg tgtttacaaa aatacttggc gatgagtctg
     4381 ccgattttat agaggcagcc attagtgaaa ttaatgctca tcatggtgat tttattaatg
     4441 gtaggtatgt aaaggtaaat tcaagagagc ggaaaatact taatatttct atccctgtta
     4501 gtggtcttga tgatttagta gatattagtt ttaaggcgtt accagaatct aagagtcgtg
     4561 ccaggcaagc cttaaaaaag gctcaaatgg ttaaaatgga ggagcgactg gaggccatga
     4621 aggatagtgc aaaattaatt gttcatacat catctgtagc tgctggggtg gttggtgcaa
     4681 ctcctgtgcc aggctctgat gctccgctta ttgctggagt gcagagtaca atgatataca
     4741 agattaattc tgaattcgaa cttgatataa caacatcagc atcagcgagt attattagtg
     4801 ggatacttgg agttaccgcg gtagcacagg tgggtaagac tattgtatct aatgttttaa
     4861 aattcattcc tggtgtgggt tccgttgttg ggggggcaat atcagcagct acagcagttg
     4921 gtcttactga ggctattggt catgcatata ttcaagttct ggctagttat tatgataaaa
     4981 c
//
