LOCUS       NZ_CP069832             4062 bp    DNA     circular CON 03-DEC-2023
DEFINITION  Klebsiella pneumoniae strain FDAARGOS_1327 plasmid unnamed7,
            complete sequence.
ACCESSION   NZ_CP069832
VERSION     NZ_CP069832.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN16357469
            Assembly: GCF_016903115.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 4062)
  AUTHORS   Sokurenko,E., Tallon,L., Sadzewicz,L., Zhao,X., Boylan,J., Ott,S.,
            Bowen,H., Vavikolanu,K., Mehta,A., Aluvathingal,J., Nadendla,S.,
            Yan,Y. and Sichtig,H.
  TITLE     FDA dAtabase for Regulatory Grade micrObial Sequences (FDA-ARGOS):
            Supporting development and validation of Infectious Disease Dx tests
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4062)
  AUTHORS   Sokurenko,E., Tallon,L., Sadzewicz,L., Zhao,X., Boylan,J., Ott,S.,
            Bowen,H., Vavikolanu,K., Mehta,A., Aluvathingal,J., Nadendla,S.,
            Yan,Y. and Sichtig,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2021) Institute for Genome Sciences, University of
            Maryland School of Medicine, BioPark II, 801 W. Baltimore St., Suite
            619, Baltimore, MD 21201, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SMRT v. 8.0.0, HGAP v. 4
            Genome Coverage                   :: 763.24x
            Sequencing Technology             :: Pacbio; Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 12/03/2023 04:05:30
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,467
            CDSs (total)                      :: 5,342
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 125
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 36 of 104
            Pseudo Genes (incomplete)         :: 63 of 104
            Pseudo Genes (internal stop)      :: 13 of 104
            Pseudo Genes (multiple problems)  :: 8 of 104
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP069832.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4062
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="FDAARGOS_1327"
                     /host="Homo sapiens"
                     /culture_collection="FDA:FDAARGOS_1327"
                     /db_xref="taxon:573"
                     /plasmid="unnamed7"
                     /geo_loc_name="USA: WA"
                     /collected_by="ARMADA"
     gene            97..351
                     /gene="mobC"
                     /locus_tag="I6K57_RS26465"
                     /old_locus_tag="I6K57_26465"
     CDS             97..351
                     /gene="mobC"
                     /locus_tag="I6K57_RS26465"
                     /old_locus_tag="I6K57_26465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase accessory protein MobC"
                     /protein_id="WP_000091744.1"
                     /translation="MSRTLEQKIADAEARLQRLKAKSRSLDTAQKVIVGAALLAKVRKP
                     EEVQLRAWLLQFLKAEVTRQADVTRILPLINELEALPEQ"
                     /name="mobC"
     gene            884..1585
                     /locus_tag="I6K57_RS26470"
                     /old_locus_tag="I6K57_26470"
     CDS             884..1585
                     /locus_tag="I6K57_RS26470"
                     /old_locus_tag="I6K57_26470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001281399.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_001281396.1"
                     /translation="MSDLIAYKSNALIEASYKLTLQEQRFLLVCISRLKSGEDSPSPDE
                     QKTMTITAAEFHDAFPDMGRQHAEARLKEAIDRLWDRSVIIKNEEKREEFRWVQYRAQY
                     FKGEAKVEITFSDAIMPYLTQLKGQFTRVVVKNVSSLSSTYSIRIYELLQQFRSTGDRT
                     IAIDDFRTMLAVEDKYQQFKDLNKMIIKPAIAELNEKSDLVVTVETIKKGRTVVALHFR
                     FKEDKQIKMTI"
                     /name="replication initiation protein"
     gene            complement(1833..3980)
                     /gene="mobQ"
                     /locus_tag="I6K57_RS26475"
                     /old_locus_tag="I6K57_26475"
     CDS             complement(1833..3980)
                     /gene="mobQ"
                     /locus_tag="I6K57_RS26475"
                     /old_locus_tag="I6K57_26475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016240513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobQ family relaxase"
                     /protein_id="WP_070749924.1"
                     /translation="MAIYHLSMKIISRSNGYSAVASAAYRSGSLMLDERTGLTHDYTRK
                     SGVAEAVILTPATAPAWCTNRAELWNAVEKAERRKNSQLAREIELAIPHELPQDAARET
                     VLAFVRENFVSQGMIADVAFHHMDKTNPHAHIMLTTRAVGPAGFGGKVRDWNDRTHAET
                     WRASWADHANRALANAGYQKEIDHRSYERQGLEKTPGIHLGKSACAMETRGIETERGEQ
                     NHLINRLNLEIQISHTELRNSGLTEPARRVADLLNIGLPDNATSYTLRDIIEALPKDSN
                     AAWKLTSNAMSMMADMQATRRRWEELNTERKEAMSHAASLKKSSPFSSGFSRIPLMQWA
                     APEYRSEQGKIQSLSQQMEKLRQHYRKLEKQDIPASQTAFEKQWQQWGESGLAALRSQL
                     LKREEELRRKELEDTERSRAEQLRKNDNAVMDSGVLRAVVTGYGEAPMPGNGEMTCYLL
                     LHNRNGEYTLWGNELEKYRTRIFESVDLMRDRSGYICDRSEIGQYPPLQRVYSSATFEQ
                     LLTQVCQTWPQHTRNLRQPKTWPESFCLGEDRQPAMPSLAARKVDFTQGRLPPTLMPVM
                     SSVDRETRQLQLLLVMGVDDSLGGVVRLNGTLYPAFAVPSADNSQLVISALTDKGLRYA
                     GYGVAVNHDADSHISPAPELMEFHLKTREAPLFAAVNTPEKQPDHLFRSLGFNRTWDEW
                     RREEDARTHATERRHDRGWSQ"
                     /name="mobQ"
     Typing          2..96
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="2.15e-42"
     Typing          complement(3623..3981)
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="97.5"
                     /coverage="100.0"
                     /evalue="8.52e-174"
     Typing          3982..4062
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="94.0"
                     /evalue="1.33e-34"
     Typing          885..1584
                     /name="rep_cluster_1778"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="99.429"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          complement(1837..4061)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="97.185"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 cgcacttcgt gatttgccgt gcattatgcc agctcagttt ttaaactacc agtgacgcgc
       61 taaaatgcga tttttcatcg cgcatggagt tacatcatga gcagaacgct cgaacagaag
      121 attgctgatg ctgaggcgag attacagcgc ctgaaggcga agagccggag cctggacaca
      181 gcgcagaagg tcattgtggg tgcagcattg ctggcaaaag tcagaaagcc ggaggaagtg
      241 cagttgcgag cttggttact ccagttcctg aaggcagagg tgacacggca ggctgatgtg
      301 acgcgaatac tgccgctgat taacgagctg gaggcgctgc cagagcagtg attgtatcgt
      361 ccgctcgccg cagcccagcg accgagcgtt agcgagcgag tgggcgagga agcggaagtg
      421 aatcagtcgc catgttgagc acatacgcac ggtttttcga caggtgatat gcgctgaacc
      481 cgcatgaata ctggatttcc acagggttgg agggggtgta ttcggaatct tcaatcctga
      541 tttttttcaa cacattgttt ttattggatt tttattttca gcaccccaag ttatccacag
      601 gctcgccgtt aagtttttaa gatcttattt ttagatcttt ttttttaaaa cctttttagg
      661 tcttgtaacc acatgatttt aaaggtgttc tggcgagata ggtggagctt ttgggggtga
      721 taggtggagc ttttgggggt gataggtgga gcttttgggg gtgataggtg gagcttttgg
      781 gggtgtaatc cacagaaaaa aggggttgca aaaaaaaaac ttccaccaat atgatgggtg
      841 gcgtacagta aaaaaacgcc accaatagca acgagcaagc gaaatgtcag atctgatagc
      901 ttacaaatca aacgccctta tcgaggcaag ctacaagctc actttgcagg aacagcgctt
      961 cctgctggtc tgtatcagcc gcctgaaatc cggagaagat tccccttcgc cggatgaaca
     1021 aaaaaccatg accatcacgg ctgcagagtt tcatgatgcc tttcctgaca tgggacggca
     1081 gcacgctgaa gctaggctca aggaagcgat agatcgcctc tgggacaggt cagtaatcat
     1141 caagaacgag gaaaaacgag aagagttccg ctgggtgcag taccgggcgc agtatttcaa
     1201 aggtgaggcg aaagtagaga tcaccttctc cgatgccatc atgccgtatc tgacacagct
     1261 caaggggcag tttacgcggg tagtcgtgaa gaacgtctcc agcctctcca gcacctacag
     1321 catccgcatt tacgagctgc tccagcagtt ccgcagcacc ggtgacagaa cgatagctat
     1381 cgatgatttc cgaaccatgc ttgccgttga agataaatat caacaattca aagacttgaa
     1441 taagatgata atcaagccag ccatcgccga actgaacgaa aaaagcgatc tggttgtaac
     1501 ggttgagacg atcaaaaagg ggcgcacggt tgtagccctg cattttcgct tcaaggaaga
     1561 caaacagatc aagatgacga tctagaatcg attttaaggc acctaaaaca ctccctgttg
     1621 cgtttttttt gtgttagtag tgcgaaggta ctattgaagt gaaaaattgc gtcaaaaacg
     1681 ctctgatgca gcgaaaaaag aacccccaat ttttgggggc ggaaaaacaa aactatgctt
     1741 gcgttatgtg agtgtgcttc ttgcattcat ccctgtcgat gaacatcgac gaacataaca
     1801 cgatgttaaa caaaacaaaa tattgttcac attcactgtg accaaccgcg atcatgtctc
     1861 ctctcggtgg cgtgtgttcg tgcatcttcc tcgcgccgcc attcgtccca ggtacgattg
     1921 aaacccagcg agcggaacag atgatccggc tgtttttctg gtgtgttgac ggcagcaaaa
     1981 agcggtgcct cacgggtttt cagatgaaac tccataagtt ccggcgcagg tgatatgtgg
     2041 ctgtctgcat catgatttac ggcaacacca taccctgcat accgtaaccc cttatcggtc
     2101 agtgcactga tgaccagctg gctgttatcg gctgagggaa cggcaaacgc gggatacagc
     2161 gtaccattaa gccggacaac accgcccaga gagtcatcca cgcccatgac cagcaaaagc
     2221 tgaagctggc gggtttcccg gtcgacagaa ctcatgaccg gcatcagcgt cggtggcaga
     2281 cgtccctgcg taaaatccac cttccttgct gccagtgatg gcatggctgg ctggcggtct
     2341 tctccaagac agaaagactc cggccatgtc ttcggttggc ggagattgcg ggtatgttgc
     2401 ggccatgtct ggcaaacctg cgtcagaagt tgctcaaagg tcgcagagga ataaacacgt
     2461 tgcagtggtg gatattgacc gatttcgctt cggtcacaga tataaccact cctgtcacgc
     2521 attaaatcaa cactctcaaa gatcctggtg cggtatttct ccagctcgtt cccccagagc
     2581 gtatattctc cgttccggtt gtgaagcaga aggtaacagg tcatttcccc gttaccaggc
     2641 atgggagcct cgccataccc cgtaacgact gctctcagca cccctgaatc cataacggca
     2701 ttgtcatttt tcctgagctg ctccgcgcga ctacgttctg tatcctccag ctccttccgg
     2761 cgcaattcct cttcacgttt aagcaactgg gatctgagag ctgcaaggcc ggattccccc
     2821 cactgctgcc actgtttttc aaatgccgtc tgactcgcgg gaatgtcctg tttctccagc
     2881 ttccggtaat gctgtcgcaa tttttccatt tgctggctca gagactggat ttttccctgc
     2941 tcactacggt attccggtgc agcccattgc atcaggggaa tacgggaaaa accggacgaa
     3001 aacggcgatg attttttcag gctggcagca tggctcatcg cttcctttcg ttcggtgttc
     3061 agctcttccc agcgtctgcg cgtcgcctgc atatctgcca tcatcgacat ggcgttactg
     3121 gtgagtttcc atgccgcgtt actgtccttc ggcaacgctt cgatgatgtc acgtagtgtg
     3181 tacgaagttg cgttgtcagg aagcccgata ttcagaaggt cagccacacg gcgggcaggc
     3241 tctgtcagcc cgctgttcct gagttcggtg tgggaaatct gtatttccag gttaagcctg
     3301 ttaatcagat gattctgttc gccgcgttct gtttcgatac cgcgtgtttc catcgcacag
     3361 gcagactttc cgaggtgaat acccggcgtt ttctcaagcc cctgtcgctc gtatgaacga
     3421 tggtctatct ctttctgata accggcgttc gccagtgccc tgtttgcatg gtcagcccat
     3481 gatgcacgcc acgtctcggc atgtgtccgg tcgttccagt ccctgacctt tccgccgaac
     3541 ccagcaggcc cgacagccct cgtggtcagc atgatgtgcg catggggatt tgttttatcc
     3601 atgtgatgga acgccacatc agcaatcatg ccctgactga caaagttttc tctgacaaag
     3661 gcgagaacgg tttcccgtgc ggcatcctgc ggcagttcgt gggggatggc gagttcaatt
     3721 tcccgcgcaa gttgtgagtt cttccggcgt tcggccttct caacggcatt ccagagttcg
     3781 gcacggttcg tacaccaggc aggggctgtc gcgggggtca gaatcacagc ctcagccaca
     3841 ccgcttttgc gggtgtagtc gtgagtcagt ccggtacgct catcaagcat tagtgaacca
     3901 gaacgatagg cagcagaagc aacggcgctg tagccgttgc ttctggagat gattttcata
     3961 ctgagatgat aaatcgccat ttctgccgta atgccttttt gcagttttct ttcagccccg
     4021 cagggcgcac acaatcgaaa cggagtggag attgtataag tg
//
