LOCUS       NZ_CP069831             4634 bp    DNA     circular CON 03-DEC-2023
DEFINITION  Klebsiella pneumoniae strain FDAARGOS_1327 plasmid unnamed6,
            complete sequence.
ACCESSION   NZ_CP069831
VERSION     NZ_CP069831.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN16357469
            Assembly: GCF_016903115.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 4634)
  AUTHORS   Sokurenko,E., Tallon,L., Sadzewicz,L., Zhao,X., Boylan,J., Ott,S.,
            Bowen,H., Vavikolanu,K., Mehta,A., Aluvathingal,J., Nadendla,S.,
            Yan,Y. and Sichtig,H.
  TITLE     FDA dAtabase for Regulatory Grade micrObial Sequences (FDA-ARGOS):
            Supporting development and validation of Infectious Disease Dx tests
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4634)
  AUTHORS   Sokurenko,E., Tallon,L., Sadzewicz,L., Zhao,X., Boylan,J., Ott,S.,
            Bowen,H., Vavikolanu,K., Mehta,A., Aluvathingal,J., Nadendla,S.,
            Yan,Y. and Sichtig,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2021) Institute for Genome Sciences, University of
            Maryland School of Medicine, BioPark II, 801 W. Baltimore St., Suite
            619, Baltimore, MD 21201, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SMRT v. 8.0.0, HGAP v. 4
            Genome Coverage                   :: 763.24x
            Sequencing Technology             :: Pacbio; Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 12/03/2023 04:05:30
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,467
            CDSs (total)                      :: 5,342
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 125
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 36 of 104
            Pseudo Genes (incomplete)         :: 63 of 104
            Pseudo Genes (internal stop)      :: 13 of 104
            Pseudo Genes (multiple problems)  :: 8 of 104
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP069831.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4634
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="FDAARGOS_1327"
                     /host="Homo sapiens"
                     /culture_collection="FDA:FDAARGOS_1327"
                     /db_xref="taxon:573"
                     /plasmid="unnamed6"
                     /geo_loc_name="USA: WA"
                     /collected_by="ARMADA"
     gene            complement(join(4590..4634,1..189))
                     /locus_tag="I6K57_RS26435"
                     /old_locus_tag="I6K57_26435"
     CDS             complement(join(4590..4634,1..189))
                     /locus_tag="I6K57_RS26435"
                     /old_locus_tag="I6K57_26435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137388.1"
                     /translation="MTELEGHLLNALEHLQQDYMRRLNEWESAFAELQKMHAVTQQNNA
                     ILNERVVNLSQQVQLLAGQVDRLSRLFITNNR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(196..756)
                     /locus_tag="I6K57_RS26440"
                     /old_locus_tag="I6K57_26440"
     CDS             complement(196..756)
                     /locus_tag="I6K57_RS26440"
                     /old_locus_tag="I6K57_26440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_000067488.1"
                     /translation="MSNLLQMGTDFEKKLKERAASTENMLNSEFRKLEESVDKALSLNR
                     QKIRDAISEHTTSVKQQLDTLSTTVSTQLSTTEAELSRQQKNLLWQVIKGRVLFPALTA
                     LSVTGGIFLGCWGLIQWQESRIAKNILTIREQENTLAKLEAKTWGVTFVNGENGKFLVL
                     PDGVKGENTWTVGDKNAVRLVRE"
                     /name="MbeB family mobilization protein"
     gene            complement(<785..1444)
                     /locus_tag="I6K57_RS27485"
                     /old_locus_tag="I6K57_26445"
                     /pseudo=""
     CDS             complement(<785..1444)
                     /locus_tag="I6K57_RS27485"
                     /old_locus_tag="I6K57_26445"
                     /inference="COORDINATES: protein motif:HMM:NF015397.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(1434..1757)
                     /locus_tag="I6K57_RS26450"
                     /old_locus_tag="I6K57_26450"
     CDS             complement(1434..1757)
                     /locus_tag="I6K57_RS26450"
                     /old_locus_tag="I6K57_26450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_001677263.1"
                     /translation="MLTIRVSDEEHARLLERCEGKRLAEWMRRVCLGEPVARTGKLPTL
                     SPPLLRHLAAIGNNLNQTARKVNSGHWSSIDRVHVVAALMAIEGELRQLRQAVREQGGR
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            1820..2011
                     /locus_tag="I6K57_RS26455"
                     /old_locus_tag="I6K57_26455"
     CDS             1820..2011
                     /locus_tag="I6K57_RS26455"
                     /old_locus_tag="I6K57_26455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165986.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGESN"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            2681..2830
                     /locus_tag="I6K57_RS27560"
     CDS             2681..2830
                     /locus_tag="I6K57_RS27560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032194431.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPERHVKTPLAAAIGNRLEELVLK
                     LCAG"
                     /name="hypothetical protein"
     gene            3022..3861
                     /locus_tag="I6K57_RS26460"
                     /old_locus_tag="I6K57_26460"
     CDS             3022..3861
                     /locus_tag="I6K57_RS26460"
                     /old_locus_tag="I6K57_26460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001354646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001354646.1"
                     /translation="MLTLFIFFVLLIAACFFCFAPPRRGYDRNEIIPYKIKLSINKYRL
                     YIYSSGKVRQYLLFLVILSLYYSIAEPFKSELIKNISYSLMAAFIFDTGLNFSKENITK
                     GVISTRWHNDLYSSFERMKAINKIYYPSNKEINTEGLSKAITSSLFNDDANSFAKRDFR
                     LMWDLSSEKYLSYKEIIIRKGDKLDAVCLRFINDDYKFLVNFNRDEEVFKYFPSIMQPS
                     LKTYRALSRLVNSIKDPSRFKFTTESLEMELLEYLELRNELFNDIEEVMGSYAQRAP"
                     /name="hypothetical protein"
     Typing          complement(2100..2184)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="3.03e-37"
     Typing          2195..2750
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.086"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          complement(3..1444)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.792"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 tccggctaat agctgcacct gctgactcaa gttcacgaca cgctcgttca gtatcgcgtt
       61 gttctgttgc gtaaccgcgt gcatcttctg caattctgcg aaggcgctct cccactcgtt
      121 cagccgccgc atatagtcct gttgcaaatg ctctaaggcg ttcagcaaat gcccttccag
      181 ttccgtcata ctcgtttact ccctgaccag cctcacggcg ttcttgtcgc ccaccgtcca
      241 ggtgttttcc cctttcaccc cgtccggcag caccagaaat ttcccgttct cgccattgac
      301 gaacgtcacc ccccaggttt ttgcctcgag tttcgccagc gtgttttcct gctctctgat
      361 ggtcaagata tttttcgcaa tcctgctttc ctgccactga atcagccccc agcaccccag
      421 gaaaatgccg cccgtgacgc tgagcgcggt cagcgccgga aacagtaccc gccctttgat
      481 cacctgccag agcaggtttt tctgctgccg ggacagttcg gcctctgtgg tgctgagctg
      541 cgtgctcacg gtggtgctca gcgtatccag ttgctgtttc acggatgttg tatgttcgct
      601 gatagcgtct ctgattttct gcctgtttaa gctcagtgcc ttgtctacag actcttctag
      661 cttcctgaac tcgctgttca gcatgttctc ggtagaagcg gctcgctctt tcagtttctt
      721 ctcgaagtct gttcccattt gtaaaagatt gctcatacag cgcccccttt agcctgagat
      781 tccgcccccc ctccgggtcg gcaatactga tactgcttct ggttgtcctc accacctcaa
      841 aacctgccgc tgtgagcgcc tcagtgacat cctgacgcgt ttttattgct ccggcacggt
      901 aaagcgcctc aagaccccgt gtaatcgctt ctgcggcctc ctgtttcgct tttggcagat
      961 tattcggtgt gacaagcgtc cgtctgttct ccggtgcgtt cgggtcacgc agcccgtaat
     1021 gatgattaac cagcgtctgc caggcattta tcctcggcct gtccgcacgg tcgtagtacg
     1081 gctggaggcg cttaccgctc tgtagttcca tgttcggaat gacaaaattc agttcgagcc
     1141 gccccttatc ctggtgttcc acccagagaa tgctgtactg gtctttttcg agaccgggca
     1201 taagaacacg ctcaaagctc gtcatcactc tttcgcgttc acccggcggt aactccttct
     1261 ccgcgaacga cagaacgcct gacgtgtatt tcttcgaaaa tggcgtggca tcaatgagtt
     1321 cccggatttc ttccgggtta ccccggagca ccgttgcgcc ttcgcggtta cgctcccgcc
     1381 ccagcaggta atcgaccggg ccacttccac cgcctttccc cctggcatga aatttaacta
     1441 tcatcccgtc ccccctgttc cctgacagcc tgccgcaact ggcgcaattc accctcgatg
     1501 gccatcagtg cggccaccac atgaacccgg tcaatcgaag accagtggcc gctgttcacc
     1561 ttccgggctg tctggttcag attgttcccg atggcagcca gatgccgcag caatggcggc
     1621 gatagtgtcg gcagttttcc tgtgcgcgca accggctccc ccagacagac ccgccgcatc
     1681 cattctgcca gtcgtttacc ctcgcagcgt tccagtaacc gggcgtgctc ctcatcgcta
     1741 acccgtatcg tgagcatctt ctcgcgtttc atcggtatca ttccccctaa aaaatcccct
     1801 attaaggagc cttaagacca tgaccaaaca ggaaaaaaca gcccttaaca tggcccgctt
     1861 tatcagaagc cagacgctga ccctgctgga aaaactcaac gagctggacg cggacgaaca
     1921 ggcagacatc tgcgaatcac ttcacgacca cgccgacgaa ctctaccgta gttgcctcgc
     1981 acgcttcggc gatgacggtg aaagcaactg aaacatgcag ctcccggaga cgttcacagc
     2041 ttgcctgtga gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag cgggttttag
     2101 cgggtgtcgg ggcgcagccc tgacccagtc acgtagcgat agcggagtgt atactggctt
     2161 aactatgtgg catcagtgcg gattataaga aaagtgcacc atatgcggtg tgaaataccg
     2221 cacatatgcg taaggagaaa atgccgcatc aggcgctctt ccgcttcctc gctcactgac
     2281 tcgctacgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa gacggtgatg
     2341 cggttatcca cagaatcagg ggataacgcc aaaagaaaca tgtgagcaaa agaccaggaa
     2401 caggaagaag gccgcgccgc tggcgttttt ccataggctc cgcccccctg acgagcatca
     2461 caaaaacaga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
     2521 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
     2581 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgttggta
     2641 tctcggcccg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
     2701 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg aaagacacgt
     2761 aaaaacgcca ctggcagcag ccattggtaa ccgtttagag gagttagtct tgaagttatg
     2821 cgccggttaa ggctaaactg aaaggacaga tttggtggct gtgctcctcc aagccagtta
     2881 ccttggttca aagagttgct gctctgagaa ccttcgaaaa gccgccttgc tgggcggttt
     2941 tttcgtttac ggacaagact accttcagga tcgttagatc tcaagacaat catgcatagt
     3001 gagcatacac aggagccgaa cttgcttaca ctgttcatct tttttgtttt attaatagct
     3061 gcatgtttct tttgttttgc tccaccacga agaggatatg acagaaatga gatcattcca
     3121 tacaaaataa aactatcaat aaataaatat cgcctttaca tatactcatc gggaaaggta
     3181 agacaatacc ttttattttt agttatttta tcactgtact actccatagc ggaaccattt
     3241 aagtctgagt taataaaaaa catatcatat tcactgatgg ctgcatttat ttttgataca
     3301 gggcttaatt ttagcaagga gaatataact aaaggggtga tttcaacaag gtggcataat
     3361 gacctatatt catcatttga gagaatgaaa gcaattaata aaatttacta cccatccaac
     3421 aaagagataa acacagaagg tctatccaaa gccatcacat catctctttt caatgatgac
     3481 gcgaactcgt tcgcaaagag agattttcgc ttgatgtggg acttatcttc tgaaaaatat
     3541 ctttcctata aagaaataat aataagaaaa ggcgataagc ttgacgctgt atgcttgaga
     3601 tttataaatg atgactacaa gtttttagtt aatttcaaca gagatgaaga ggttttcaaa
     3661 tactttccat ctataatgca accttcactc aaaacatata gagcgttatc aagactagta
     3721 aactcaatca aagatccttc tagatttaag ttcacgacag agtcattaga aatggagtta
     3781 ctagaatact tagagttaag aaatgaatta ttcaatgaca tagaagaagt aatgggtagc
     3841 tatgctcaga gggcaccata gttcatgata gccctcgata gtacttactt atatttttag
     3901 agaggtttaa gtgtaagtag agggagtaac acctggtttt tcagcatcca ctccaatgaa
     3961 tgctgacacc gatacgcgat aattctcttt atcgtagcaa aatcatcctc cagattgcgg
     4021 ttgagaatag gttcatggtg cgcaatacga ttgcgcagtt ttctcacggg acttgttagc
     4081 agtaatgctt cccccccggg gacaaacgac agagatatac aacaaaggcc cgctgtatag
     4141 agtacagcgg gcctttgttg ttttaattcc cgcaattaaa atactccgta tttaccataa
     4201 cgtcgcattg cgcgtaccgc ttaaacgcgt tcagcgcgat cacggcagca gacaggtaaa
     4261 aacagcaaca aaccacccga aaaactgccg cgatcgcgcc tggtaaattt taacctcatg
     4321 catagttatg cgcgaaactg aatgacgatc agtttcgcac gttccagccg tcgaacgggg
     4381 caaaatgggg aggtttggat attgttaatg ctaatgtacg tacattaaca ttggcgccat
     4441 ccgggtaggg cgtgccacaa actggctcga tttcaggggg caccccggtc acatccccat
     4501 tgacgggcca cgccagatcc gcgcttcggg ttcctgctct tcatgccagc gccgggcaag
     4561 ctcctgcgcc tgacgctcct gttcatccct tatctgttgt tcgtgataaa taaccgactc
     4621 aagcggtcga cttg
//
