LOCUS       NZ_CP069830             5167 bp    DNA     circular CON 03-DEC-2023
DEFINITION  Klebsiella pneumoniae strain FDAARGOS_1327 plasmid unnamed4,
            complete sequence.
ACCESSION   NZ_CP069830
VERSION     NZ_CP069830.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN16357469
            Assembly: GCF_016903115.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 5167)
  AUTHORS   Sokurenko,E., Tallon,L., Sadzewicz,L., Zhao,X., Boylan,J., Ott,S.,
            Bowen,H., Vavikolanu,K., Mehta,A., Aluvathingal,J., Nadendla,S.,
            Yan,Y. and Sichtig,H.
  TITLE     FDA dAtabase for Regulatory Grade micrObial Sequences (FDA-ARGOS):
            Supporting development and validation of Infectious Disease Dx tests
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5167)
  AUTHORS   Sokurenko,E., Tallon,L., Sadzewicz,L., Zhao,X., Boylan,J., Ott,S.,
            Bowen,H., Vavikolanu,K., Mehta,A., Aluvathingal,J., Nadendla,S.,
            Yan,Y. and Sichtig,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2021) Institute for Genome Sciences, University of
            Maryland School of Medicine, BioPark II, 801 W. Baltimore St., Suite
            619, Baltimore, MD 21201, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SMRT v. 8.0.0, HGAP v. 4
            Genome Coverage                   :: 763.24x
            Sequencing Technology             :: Pacbio; Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 12/03/2023 04:05:30
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,467
            CDSs (total)                      :: 5,342
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 125
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 36 of 104
            Pseudo Genes (incomplete)         :: 63 of 104
            Pseudo Genes (internal stop)      :: 13 of 104
            Pseudo Genes (multiple problems)  :: 8 of 104
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP069830.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5167
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="FDAARGOS_1327"
                     /host="Homo sapiens"
                     /culture_collection="FDA:FDAARGOS_1327"
                     /db_xref="taxon:573"
                     /plasmid="unnamed4"
                     /geo_loc_name="USA: WA"
                     /collected_by="ARMADA"
     gene            join(4708..5167,1..467)
                     /locus_tag="I6K57_RS26410"
                     /old_locus_tag="I6K57_26410"
     CDS             join(4708..5167,1..467)
                     /locus_tag="I6K57_RS26410"
                     /old_locus_tag="I6K57_26410"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="WP_001331297.1"
                     /translation="MATYHLSVKFGGKGQAANHADYIERKEKYRDRQDLEYSAHGNMPE
                     WARDNPSHFWQAADQFERANGSTYRELEIALPRELTPEQRLELVQDFVRQEAGERHAWS
                     FAIHNPKASIDGGEQPHAHIMMSQRVNDGIERTPEQYFRRYNARYPERGGAKKDSGSLT
                     LTQQKEQLRELRKRWEVKHNEHMRKHGFERGFIDCRTLKEQGIERRPEVHLGFEAAGRL
                     DDAQRHDIMQKRSEPDYSRHLEQARTVGHDIREWLDTPVKQHELVRRLQHDVAREHGFD
                     MRDRVSRQNRTAPERVKSPSRGFSMGF"
                     /name="MobA/MobL family protein"
     gene            470..1003
                     /locus_tag="I6K57_RS26415"
                     /old_locus_tag="I6K57_26415"
     CDS             470..1003
                     /locus_tag="I6K57_RS26415"
                     /old_locus_tag="I6K57_26415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000168770.1"
                     /translation="MASTEEKLLTLLLSVEHLQHTAMEQQKAVADSLAVARETLRGYRD
                     ISDGVAKQVRDEVRNEVQKLDVAGLIGERISQDFQRLEESVANMKANADALNRDIATIH
                     SNIVQEYNTLKGYSWKWLGGVSVAFLVMFCVLGWGMKTILDRNYNATAAVYQKVVTLED
                     KLVEKPVTGKSRKN"
                     /name="hypothetical protein"
     gene            complement(1069..1278)
                     /locus_tag="I6K57_RS26420"
                     /old_locus_tag="I6K57_26420"
     CDS             complement(1069..1278)
                     /locus_tag="I6K57_RS26420"
                     /old_locus_tag="I6K57_26420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001618649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001096196.1"
                     /translation="MAKLGENTPHIIDATVDFMASSQAFREYLKKLPPDNTIPPALTDE
                     HVPLYIRRLEYYRLLYRPKQTESY"
                     /name="hypothetical protein"
     gene            3015..3929
                     /locus_tag="I6K57_RS26425"
                     /old_locus_tag="I6K57_26425"
     CDS             3015..3929
                     /locus_tag="I6K57_RS26425"
                     /old_locus_tag="I6K57_26425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_001271348.1"
                     /translation="MSAALQYFDENLPHRPYHTDDLAFGLRISGKGRALLARYIQQNQP
                     HAQFWLVFDVDREGAAIDWSDRNAPAPNITVKNPVNGHAHLLYALNIAVRTAPDASVKA
                     LKYAAAVERSLCEKLCADVNYSGLICKNPFHLEWLVMEWREEAYTLDELADYLDLSASE
                     RRSIDKHYGMGRNCHLFEMTRKWAYRAIRQGWPAFSQWLEAVIQRVEMYNASLPVPLSL
                     AECRAIGKSIAKYTHRNFTPETFAQYVADTHTPEIQAARGRKGGSKSKRSTVATSARTL
                     KPWEALGISRAWYYQLKKRGLVE"
                     /name="replication initiation protein"
     gene            complement(4133..4531)
                     /locus_tag="I6K57_RS26430"
                     /old_locus_tag="I6K57_26430"
     CDS             complement(4133..4531)
                     /locus_tag="I6K57_RS26430"
                     /old_locus_tag="I6K57_26430"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001222914.1"
                     /translation="MRISNIEWLKKRIGFIRKLGEQTARQRQIIDLIDNEAGLTEQERK
                     LLHVLATAEKNDLQAQESERKQAVQKRIEGKKQRRERNHRLFLAAGLLIEAGLVDTKTG
                     ELCYKKDRILQALKELKYDLETSPNPDA"
                     /name="hypothetical protein"
     Typing          3016..3790
                     /name="Col156"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="96.778"
                     /coverage="83.0"
                     /evalue="0.0"
     Typing          4..464
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="97.403"
                     /coverage="99.0"
                     /evalue="6.82e-93"
     Typing          4709..5166
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="98.693"
                     /coverage="99.0"
                     /evalue="5.63e-97"
ORIGIN
        1 gtgcgaaaaa ggattccgga agcctgactc tcactcagca aaaagagcag ttgcgggaat
       61 tgcgaaaacg ctgggaggtg aagcacaacg aacatatgag aaagcatggc tttgaaaggg
      121 gcttcattga ctgccggacg ctgaaagaac agggcattga acgacgtccc gaagtgcatc
      181 tgggttttga agctgccgga cggcttgatg atgcccagcg tcatgacatc atgcaaaaac
      241 ggtctgaacc ggactattcc cgccatcttg aacaggcgcg aacggtcggg catgacatca
      301 gggaatggct ggacactccg gttaagcaac atgaactggt cagaaggctg caacatgatg
      361 ttgcgcggga gcatggcttt gacatgagag acagagtgag cagacagaac cgaactgcac
      421 ctgaacgggt taaatcaccg tcacgtggat tttctatggg attctgaaca tggccagcac
      481 agaagaaaaa ttacttacgc tcctgttatc ggtggagcat ctgcaacata ccgcaatgga
      541 gcaacagaaa gctgttgctg attctctggc tgtggcaagg gaaactcttc gcggatatcg
      601 ggatatttct gacggtgtgg caaaacaggt cagggatgaa gtcagaaatg aggtgcagaa
      661 gctggacgtg gcaggactga taggtgagcg catcagccag gattttcagc ggctggaaga
      721 gtcagtcgca aacatgaagg caaacgcgga tgccctgaac agggatattg cgacaattca
      781 cagcaatatc gtgcaggaat acaacaccct taaagggtat agctggaaat ggctgggtgg
      841 ggtgtcagtg gcttttctgg ttatgttctg tgtgctcgga tggggaatga aaacaatact
      901 cgacaggaac tacaacgcca cagcggcggt gtatcagaaa gttgtgacac tggaagataa
      961 actggtggag aaacctgtca cggggaagag ccggaaaaac tgacgcgctt cgcttgtcca
     1021 acgcctcacg acccgcgaaa gaatttcgcg aaccgttcac cgtttttttc aatatgattc
     1081 tgtctgcttt ggtcgataaa gcagacgata gtattccagc cttcgtatat ataacggtac
     1141 gtgctcatca gtaagagctg gcggtattgt gttatcaggg ggaagttttt tcagatactc
     1201 acgaaatgcc tggcttgatg ccataaaatc tacagtagca tcgataatat gtggggtgtt
     1261 ttcacctaat tttgccatga tgataatcct tatattatca cggtttgtga atgatagggt
     1321 gtcactatta tttatgcgtt agtgaaccag aaaaaatccc ccgttggcat agggggatat
     1381 gtaaggagaa ataacttcgc aggtgttctt gtttgtataa cgtgaaaaga ctgcacgcaa
     1441 cttaataagt ctgccgtact tactttcagt tttcaacata aggtttataa caatgtatgg
     1501 aatggaagtg agctttaagg tttcagctct aaaaaataaa atatttatta gttggctggt
     1561 tgttgttctt gttctgtatg tatataaaca ctatcgacat attatgaccg ggaacattcc
     1621 ttaccccctc tgcgctcgca gaggtttttt ttgtcattaa ttaataatga actgaaatgc
     1681 agttccgata cctccatttg cacattatcc ctgacatatg gtaccggaac cgcattaatt
     1741 ttatgttata agagtttatt tttaatgtcc agatggttta atagtattta tatgtaatta
     1801 gctgtatgga tagtactggt tttattgtat aacttgtcgt ttttttttat tgtttttgtg
     1861 tggtgtgcta atctttaagt tataaaaaaa ccccttggaa tacaggggca aaagtaagga
     1921 aaacaaatgg atgctcagtt tgtgtgacac tttaaactaa tggaactata gagtggaggt
     1981 tgttacagtc gcttttataa aacagcaacc agtttttttc aatagtctga tgatggttgt
     2041 gacagttttg tgatattgtt caaaatgttt cttgttgaat gtttgttttt tggtctattg
     2101 gtgtctggtt ttattttttc tgctattaca tatcaagaaa tgtgattggt gtagcaagag
     2161 tggaatgcga tgtggataaa ctaattggtt gtcgaatcta atttacttag aaaactacac
     2221 ttgtttttgt gtatgaattt agtgaatttg tctgtctatg tgttttcgtg catttataaa
     2281 tccttcgtca ggatcttaaa tttagtgttg aatatgtagg gcctgtacgg taattattat
     2341 ttagcgtagc tacttcatcg ttgttcgaac acaaccaaca acctgtaatt gttgtttctc
     2401 ttggttcccc acttttgtgg ggttttttat ctcttccgtg catcgtaaag cgcggactcc
     2461 ttctggaaag gttgttttag ggcatcactg aggcattttt tgcgtttgtg aatattgagc
     2521 atccgataac taaaatattt cccatagact ctgattcttg ccaccgaccc caggtcggtt
     2581 tttttttgtc ctggcgagat gtgaagtttg gaaacaggaa accccgtcag agcgccacgc
     2641 accaggggag aaaacttgtc gttttgagcc gttcggtgtt cagaacgcac gaaaccgatc
     2701 gcacatcgct ttcgtgcata gttatgcaac gccctgaaaa cgattctgac gcgtttttct
     2761 ggtttggcct ggtgttttct tgtctttttg cgttttttgc gccagaaagc gactgagggc
     2821 gttttaaggg gggcgtacaa cgggagttat ggtaaatgga tcgggttttc gggaaggttc
     2881 gacaggattt gccgttgggt gtagtgtaag cgactgaaaa acaaacgccc ctgaaatcat
     2941 gtccagatcc ggcaagattc aacatgaaat acagagggcg tgtaggtcgc ataacccgga
     3001 gagaatcgta acacatgagc gccgcgcttc aatacttcga cgaaaattta ccccatcgtc
     3061 cctatcacac ggatgatctc gcttttggtc ttcgcatctc cggcaaaggg cgtgcgcttc
     3121 ttgcgcggta catacagcag aaccagcctc atgcgcagtt ctggctggtt tttgacgttg
     3181 accgcgaggg ggctgcgatt gactggagcg accggaacgc tcccgcgccg aacatcaccg
     3241 ttaaaaatcc tgtgaacgga cacgctcacc tgctctacgc actcaacatc gccgtgagaa
     3301 ccgcgcctga tgcctcggtt aaggcgctga aatatgccgc agcggttgaa cgttcgctgt
     3361 gtgagaaact ttgtgcggat gtgaactaca gcggcctgat ttgcaaaaat ccgttccacc
     3421 tggaatggct ggtgatggag tggcgcgagg aagcctatac cctcgatgaa ctggctgatt
     3481 atctcgattt gagcgcctca gagcgtcgta gcatcgataa acattacggg atggggcgaa
     3541 actgccacct gttcgaaatg acgcgcaaat gggcttacag ggcgattcgt cagggctggc
     3601 cagcgttctc acagtggctt gaagcggtga tccagcgtgt cgaaatgtac aatgcatcgc
     3661 ttcccgttcc gctttcactg gctgaatgtc gggctattgg caagagcatt gcgaaataca
     3721 cgcacaggaa cttcacgccg gaaactttcg cacagtatgt ggctgatacg cacacgccag
     3781 aaattcaggc tgcacgcggt cgcaagggcg gttctaagtc taagcgcagc acagtagcta
     3841 catctgcacg cacgctgaaa ccgtgggagg cattagggat cagtcgcgcc tggtattacc
     3901 aactgaaaaa acgaggtctc gtagagtaga ccaaataagc ctatatcaga taacagcgcc
     3961 tttctggcgt ctttttgagc agcaggtttt ttgccgtttt tccccgttca gccctgcaat
     4021 ggcgcggctt tccgcgtgat tgtggtggta gtgctcgccg cagtctcatg accgagcgta
     4081 gcgagcgaat gagcgaggaa gcaccaggga atcggaacac ggatggaggc acttacgcgt
     4141 ctgggtttgg ggatgtctcc agatcgtact taagttcctt aagtgcttgt aatatcctgt
     4201 cttttttata gcatagctcg cctgtttttg tgtctaccag tcctgcttcg atcagtagcc
     4261 cagccgcgag aaagagccgg tgattgcgtt cccgtcgttg tttttttccc tcgattcgtt
     4321 tttggaccgc ctgttttcgt tcgctctcct gtgcctgtaa gtcgtttttt tccgctgttg
     4381 ccagtacgtg gagaagtttg cgttcctgtt ctgtgagtcc ggcttcgttg tcgatcaggt
     4441 caataatctg tctctgtcgt gcggtctgtt ctccgagctt cctgatgaat ccgatccgtt
     4501 tcttcagcca ttcgatgttg ctgatgcgca tgttcgtgat gctccctggt gtttttttgc
     4561 ggtgtgttgt ggtcgtgcag tatatcgctt gcggtcggcg ggtgcagggg gtatcttccg
     4621 tgctaattcg taaaattaga aggaagcgca cttatatgtt tctctccttc gtcgagaaac
     4681 agtgcgctcc tgcagagggc tgaaaccgtg gcaacctatc acctcagcgt taaatttggt
     4741 ggcaaggggc aggctgcaaa ccacgccgat tacatcgagc gcaaagagaa ataccgcgac
     4801 cgtcaggatt tggaatattc cgcacacgga aacatgccgg aatgggccag ggataatcct
     4861 tcccatttct ggcaggcggc tgaccagttt gaacgtgcga acggttcgac ctatagggaa
     4921 cttgaaattg cattaccccg tgaactgaca ccggaacagc gccttgaact ggtgcaggat
     4981 tttgtacgtc aggaagctgg cgaacggcac gcctggagtt ttgccattca caatccgaaa
     5041 gcgagcattg acggcggcga acagcctcat gcgcatatca tgatgagtca gcgtgtgaat
     5101 gatggcattg aacgtacacc ggaacagtat ttccggcgct ataacgccag gtatcccgag
     5161 cggggag
//
