LOCUS       NZ_CP069823             9294 bp    DNA     circular CON 03-DEC-2023
DEFINITION  Klebsiella pneumoniae strain FDAARGOS_1326 plasmid unnamed4,
            complete sequence.
ACCESSION   NZ_CP069823
VERSION     NZ_CP069823.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN16357468
            Assembly: GCF_016903155.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 9294)
  AUTHORS   Sokurenko,E., Tallon,L., Sadzewicz,L., Zhao,X., Boylan,J., Ott,S.,
            Bowen,H., Vavikolanu,K., Mehta,A., Aluvathingal,J., Nadendla,S.,
            Yan,Y. and Sichtig,H.
  TITLE     FDA dAtabase for Regulatory Grade micrObial Sequences (FDA-ARGOS):
            Supporting development and validation of Infectious Disease Dx tests
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9294)
  AUTHORS   Sokurenko,E., Tallon,L., Sadzewicz,L., Zhao,X., Boylan,J., Ott,S.,
            Bowen,H., Vavikolanu,K., Mehta,A., Aluvathingal,J., Nadendla,S.,
            Yan,Y. and Sichtig,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2021) Institute for Genome Sciences, University of
            Maryland School of Medicine, BioPark II, 801 W. Baltimore St., Suite
            619, Baltimore, MD 21201, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SMRT v. 8.0.0, HGAP v. 4
            Genome Coverage                   :: 1046.3x
            Sequencing Technology             :: Pacbio; Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 12/03/2023 03:43:46
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,440
            CDSs (total)                      :: 5,318
            Genes (coding)                    :: 5,186
            CDSs (with protein)               :: 5,186
            Genes (RNA)                       :: 122
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 132
            CDSs (without protein)            :: 132
            Pseudo Genes (ambiguous residues) :: 0 of 132
            Pseudo Genes (frameshifted)       :: 59 of 132
            Pseudo Genes (incomplete)         :: 75 of 132
            Pseudo Genes (internal stop)      :: 20 of 132
            Pseudo Genes (multiple problems)  :: 20 of 132
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP069823.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..9294
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="FDAARGOS_1326"
                     /host="Homo sapiens"
                     /culture_collection="FDA:FDAARGOS_1326"
                     /db_xref="taxon:573"
                     /plasmid="unnamed4"
                     /geo_loc_name="USA: WA"
                     /collected_by="ARMADA"
     gene            join(7964..9294,1..634)
                     /locus_tag="I6K56_RS26655"
                     /old_locus_tag="I6K56_26660"
     CDS             join(7964..9294,1..634)
                     /locus_tag="I6K56_RS26655"
                     /old_locus_tag="I6K56_26660"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="WP_004178196.1"
                     /translation="MFNTDSLAWKWHIPELARQIYPWVSWLYQEYYGLAGAFLVIGLFF
                     GLAELWAGRMRFVGLSHKERREYADRYKRDNKRPAPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIAWCAGGLA
                     VGIVLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSSEQARQEQAVFLGKDEQGKPVLVPRDTWRKTNIQILGLPGSGKSVMATNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLI
                     AGLNLGETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     RLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFREWPHVSVMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPAEFV
                     KTTVLDNTPIRWFYRAASLESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARMAFSSPLRVERREIPLQRFPVLAKTDPLAEF
                     RPPADGGGPKADDDFAGIY"
                     /name="TraM recognition domain-containing protein"
     gene            634..1365
                     /locus_tag="I6K56_RS26660"
                     /old_locus_tag="I6K56_26665"
     CDS             634..1365
                     /locus_tag="I6K56_RS26660"
                     /old_locus_tag="I6K56_26665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-relaxation family protein"
                     /protein_id="WP_163566379.1"
                     /translation="MALELYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRTKYPVDGRQVSVWGLTPHGVAFSFDEDEPLADVIPFQP
                     SRVSAAHLPHRLAVQSLRLDMEARGATGWRILHRLSLKGMKVPDALAELDGKTVAFEVE
                     CTVKSRRRYQEVVSGYLFNRKANGIDEIWYICPDRATQVRVQRAILSVDEIVNPQTGEA
                     RKTAELDRERLFACFKFMTTE"
                     /name="replication-relaxation family protein"
     gene            1372..1902
                     /locus_tag="I6K56_RS26665"
                     /old_locus_tag="I6K56_26670"
     CDS             1372..1902
                     /locus_tag="I6K56_RS26665"
                     /old_locus_tag="I6K56_26670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004153058.1"
                     /translation="MALYMVFIEDAEDRNGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKATYFFTGKNPQWERNKPTGMKLCDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQINAMIDVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRWWPFGKK"
                     /name="hypothetical protein"
     gene            complement(1930..2109)
                     /locus_tag="I6K56_RS26670"
                     /old_locus_tag="I6K56_26675"
     CDS             complement(1930..2109)
                     /locus_tag="I6K56_RS26670"
                     /old_locus_tag="I6K56_26675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165971.1"
                     /translation="MTKQEKTALNMAKFIKEQSLLLLDKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGEE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            2178..2555
                     /locus_tag="I6K56_RS26675"
                     /old_locus_tag="I6K56_26680"
     CDS             2178..2555
                     /locus_tag="I6K56_RS26675"
                     /old_locus_tag="I6K56_26680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000312627.1"
                     /translation="MAWVTVRQATELTGKARSSLYRDMAKGRVSYRTEADGGRVVDTSE
                     LIRVYGELRQDETHERDELRLSGETEKMASDALIAEIKALREEVAGLRQEMQSMRLLEH
                     KPETAETPAGTPRRWWPWGKR"
                     /name="hypothetical protein"
     gene            complement(2617..2916)
                     /locus_tag="I6K56_RS26680"
                     /old_locus_tag="I6K56_26685"
     CDS             complement(2617..2916)
                     /locus_tag="I6K56_RS26680"
                     /old_locus_tag="I6K56_26685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001146176.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_001146176.1"
                     /translation="MPYDVEWKQGAIEDVTTLFDYIAENSSLWDAQNVTDRVLAAADKL
                     ADFPRLYEVDSRYGEDVRRISLMGQHVLYDVDDQTRKVRVLAVVGQRQNPHIVL"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(2906..3169)
                     /locus_tag="I6K56_RS26685"
                     /old_locus_tag="I6K56_26690"
     CDS             complement(2906..3169)
                     /locus_tag="I6K56_RS26685"
                     /old_locus_tag="I6K56_26690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000072676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000072676.1"
                     /translation="MSNTTHYENANFLRELAESLPRILPEGGPDKAALLQRLANEELAQ
                     AEYEDQVRAKVTAARADTRPGMTTEQLRQRLHGRYQELRDAV"
                     /name="hypothetical protein"
     gene            complement(3475..3894)
                     /locus_tag="I6K56_RS26690"
                     /old_locus_tag="I6K56_26695"
     CDS             complement(3475..3894)
                     /locus_tag="I6K56_RS26690"
                     /old_locus_tag="I6K56_26695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="WP_001254601.1"
                     /translation="MRRSGSKLCLIAMITATATLTACAPKGSVEQHTRHYVYASDDGFD
                     PNFSTQKADTTRMMVPFFRQFWDMGAKDKATGKSRSDVQQRIQQFHSQEFLNSLQGTTQ
                     FAGTDYRSKDLTPKKSRLLADTISAVYLDGYEGRQ"
                     /name="Exc2 family lipoprotein"
     gene            4297..5982
                     /locus_tag="I6K56_RS26695"
                     /old_locus_tag="I6K56_26700"
     CDS             4297..5982
                     /locus_tag="I6K56_RS26695"
                     /old_locus_tag="I6K56_26700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like bacteriocin tRNase domain-containing
                     protein"
                     /protein_id="WP_004178188.1"
                     /translation="MSGGDGRGPGNSGLGHNGGQASGNVNGTSGKGGPSSGGGTDPNSG
                     PGWGTTHTPNGDIHNYNPGEFGNGGSKPGGNGGNSGNHSGSSGGGQSSATAMAFGLPAL
                     ATPGAEGLALSVSGDALSAAVADVLAALKGPFKFGLWGIAIYGVLPSEIAKDDPKMMSK
                     IVTSLPADTVTETPVSSLPLDQATVSVTKRVADIVKDERQHIAVVTGRPMSVPVVDAKP
                     TKRPGVFSVSIPGLPSLQVSVPKGVPAAKAPPKGIIAEKGDSRPAGFTAGGNSREAVIR
                     FPKESGQKPVYVSVTDVLTPAQVKQRLEEEKRRQQAWDAAHPEEGLKREYDKAKAELDA
                     EDKNIATLNSRIASTEKAIPGARAAVQEADKKVKEAEANKDDFVTYNPPHEYGSGWQDQ
                     VRYLDKDIQNQNEKLKAAQTSLNEMNESLSRDKAALSGAMESRKQKEKKAKDAENKLNE
                     EKKKPRKGTKDYGHDYFPDPKTEDIKGLGELKEGKPKTPKQGGGGKRARWYGDKKRKIY
                     EWDSQHGELEGYRASDGEHLGAFDPKTGKQVKGPDPKRNIKKYL"
                     /name="colicin-like bacteriocin tRNase domain-containing
                     protein"
     gene            5992..6249
                     /locus_tag="I6K56_RS26700"
                     /old_locus_tag="I6K56_26705"
     CDS             5992..6249
                     /locus_tag="I6K56_RS26700"
                     /old_locus_tag="I6K56_26705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cloacin immunity family protein"
                     /protein_id="WP_004152553.1"
                     /translation="MGLKLHIHWFDKKTEEFKGGEYSKDFGDDGSVIESLGMPLKDNIN
                     NGWFDVEKSWVSILQPHFKNVIDISKFDYFVSFVYRDGNW"
                     /name="cloacin immunity family protein"
     gene            6334..6483
                     /locus_tag="I6K56_RS26705"
                     /old_locus_tag="I6K56_26710"
     CDS             6334..6483
                     /locus_tag="I6K56_RS26705"
                     /old_locus_tag="I6K56_26710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891188.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_004152552.1"
                     /translation="MKKVKVVFLFILIFSGFLLVACQANYIRDVQGGTVAPSSSSELTG
                     IAVQ"
                     /name="colicin release lysis protein"
     BGC             1..9294
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          complement(6587..7165)
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.361"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          635..1362
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.588"
                     /coverage="100.0"
                     /evalue="1.97e-149"
ORIGIN
        1 aatgctgttt gcccgctgcg cgcaaatcat cattgcgcgg gacgagttcc gggagtggcc
       61 acacgtcagc gtgatgctcg atgaaattaa atatctgctg tcgaagtatg tactgaacgc
      121 actggggacg ctgcgcagcc gggactgcaa tctgttgctc gcgcaccagt cgctgggcga
      181 tttcgggcag tgcgggcagg atctgcccgc tgaatttgtg aaaaccacgg tactggataa
      241 cacgccgatc cgctggtttt accgggcggc cagcctggag tccgcgcagt gggcggcggg
      301 gcagaccggg gaaatcaggg tggacgtgga gcgccgccgt gccagccggg aggcggggaa
      361 cgtggagcat atcagcgggg acagcttcat ccagaaggag gcgcggccgc tgtttgacgt
      421 gaacaccctg caacacctgc cggacgggtt cgcggtcatg accgggctgg gtgtggcccg
      481 gatggcgttc agcagtccgc tgcgggttga gcgccgggaa atcccgttgc agcgtttccc
      541 ggtgctggcg aaaaccgatc ccctggcgga gttccggccc ccggccgacg gcggcgggcc
      601 gaaagcggat gatgattttg cggggattta ctgatggcgc tggagctgta caacgtcagc
      661 cacgcgaaac gacaggcgcg gaacgcggag aaaacccggc tgaccctgcg ctggttgcgg
      721 gaagagctgt gttccacggc ggagctggtt gcgcggcggc tggggattgc ggcggtgcag
      781 ccggtctacc gcttcctcga cagcctggtg gcgaaagggc tgctggtccg gaccaaatat
      841 ccggtggacg gccgtcaggt cagcgtgtgg gggctgacgc cgcatggcgt ggcgttctcg
      901 ttcgacgagg acgaaccgct ggcggatgtt attccgtttc agccgtcaag ggtgtcggca
      961 gcacatctgc cgcaccggct ggccgtgcaa tccctgcgcc ttgatatgga agccagaggg
     1021 gccaccggct ggcgcattct gcaccgtctg agtcttaaag gcatgaaagt gccggacgcc
     1081 ctggcggagc tggacggtaa aacggtggcg tttgaggtgg agtgcaccgt caagagccgt
     1141 cgccggtatc aggaagtggt gtccgggtat ctgttcaacc gcaaagctaa cggcatcgat
     1201 gaaatctggt acatctgccc ggaccgggcg acgcaggtca gggtgcagcg ggcaatcctg
     1261 tcggtggatg aaatcgtgaa cccacagacc ggcgaggccc gcaaaacggc agagctggat
     1321 cgtgaacggt tgtttgcgtg ctttaaattt atgacaacgg agtgagggat tatggcgctg
     1381 tacatggtat ttattgaaga cgcggaggac cggaacggcg agcgctgcat catgccgacc
     1441 agcgagcatc tggaagatat ggtggtgctc gcctggcaga ccggggcgta tgttttctgt
     1501 tttgtcgacc gggcccagca tctggcgatg atgaaatcca tcgggatgta tgacgagcgg
     1561 ctcaacagcg acgaaaaagc cacgtacttt ttcactggta aaaatccgca atgggaacgg
     1621 aacaaaccca caggaatgaa gctgtgtgat tacctctatc gcaaggtgga gcggttcgcc
     1681 atcgagaacg gtgtgcaggt ggttgatgcc gggaagtgct caccggagca gattaacgcg
     1741 atgattgacg tggtgagtga gcggcacggg gaaagcgcgg tgaacgagaa gctgaaagcg
     1801 gagctgatgc gggaatataa cagccgctat tttaacgtgc cggtacctga actgccggag
     1861 gacaaacctg cccgccggtg gtggccgttc ggcaaaaaat aaatgccagc accggccatg
     1921 ccggtgctgt tattcctccc cgagcttatc ccgcatcacc tgccagagtg tttccgcatc
     1981 ctcgtgtagc cgctcgcaca tgtcggccgg ggtatccata tccagctcat tcagtttatc
     2041 gagcaatagc agcgactgct ccttaataaa tttagccata ttgagggctg ttttttcctg
     2101 ttttgtcatg attctttcct tccgggcatg gtgtgcgcct gtcaggtata ctgtctcaaa
     2161 aatagcgggg tgagacaatg gcgtgggtga cggtgagaca ggcgacggaa ctgacgggta
     2221 aagcccgaag ttccctgtat cgggatatgg cgaaaggccg cgtatcatac cgtacggaag
     2281 ctgatggcgg ccgggtggtg gataccagcg agcttatccg ggtgtacggt gagttaagac
     2341 aggatgagac tcacgagcgg gacgagttga gactgtcggg tgagactgaa aaaatggcct
     2401 ccgacgcgtt gatcgcggag ataaaagcac tcagggagga ggtggccggg ctacggcagg
     2461 aaatgcagtc catgagactg ctggaacaca aaccggagac agcagagacc ccggccggaa
     2521 caccgcgccg gtggtggccg tggggaaaac gctgacgcgc tgtgcttgtc caaccggcga
     2581 cggccacgca atgaattgcg tccccgtcac cggttattag agaacaatat ggggattctg
     2641 gcgctggccg accacggcca gaacccggac ttttctggtc tggtcatcaa catcatagag
     2701 gacatgctgt cccatcagac tgatccggcg aacgtcctcg ccatagcgtg agtccacttc
     2761 atagagtcgc gggaagtcgg ccagtttgtc cgccgcagcg agaacccggt cggtaacgtt
     2821 ctgcgcatcc cacagggatg aattttccgc gatatagtca aacagcgtcg ttacatcctc
     2881 gatggctccc tgcttccatt ccacgtcata cggcatcgcg cagttcctga taacggccat
     2941 gcaggcgctg gcggagttgt tccgtggtca tacccggacg cgtgtcagca cgggcggcgg
     3001 tcactttggc gcgaacctga tcttcatact ctgcctgggc cagttcttcg ttagccagtc
     3061 gctgcaacag cgcagctttg tccgggccac cttccggcag tatgcggggc aggctttcag
     3121 ccagttcccg aagaaagttg gcgttttcgt agtgtgtagt gttgctcatt gcggagcctc
     3181 ccgggttaat gcggcaatat gaaggaagtt tagcaggggg cggtctgaaa gaatatggtc
     3241 gtgtcagtca ggctgatgaa gcagaaagtc ggtaaaaaag ggcttacggc atccatttta
     3301 cgtcaaaaca tatgccatgc agttgcgtgc ataggcatgc attaggataa aatttatcgg
     3361 gcgcgtttcc ggcagttttc cgggggggtt gttgcctgtt ttatcccgta gccgccggaa
     3421 acgccctgag cccgtctgag cggtgcgcgt aatgacgcgt tatggtaaat ccacttactg
     3481 tctgccttca tagccatcga gataaacagc tgaaatggtg tcagccagta gccttgattt
     3541 tttcggtgtg aggtctttgc tgcggtaatc ggtacccgca aattgtgttg tgccttggag
     3601 tgagtttaaa aattcctgac tgtgaaactg ctgaatgcgt tgctgcacat cactccgtga
     3661 ttttccggtc gctttatcct ttgcgcccat atcccagaac tgccggaaaa aaggcaccat
     3721 cattcgtgtt gtgtcagctt tctgggtgga aaaattggga tcaaaaccgt catctgaagc
     3781 atatacgtaa tgccgggtat gctgttccac gctgcccttt ggagcacagg cagtgagagt
     3841 tgctgtcgct gttatcatgg cgataagaca cagtttgctg ccggaacgtc tcatgagcgt
     3901 ctccttacct ttaattcttt tttctcattg agttatatat ccgcaggcta tcttatcatc
     3961 agtttctgac cgtgttcagt gaccgaacaa cccgaaccag accccccgtt tcttttcctg
     4021 tcccggaggg ggctttctgg tatctcatat aatgatttca gtaaaacata ctgaggccgg
     4081 agaacggcgc cgtttgtgct ggtttttgtt gcatctggag gggattgcgc atggtgtgag
     4141 tggctgtttt tttgatcatt ttcacaaaaa cgcaaattta cagacttgac atgaaaaaca
     4201 aggcagcgta atttttatac tgtatataaa accagtggtt atatgtacag tattttattt
     4261 tagtttattg ttttaaaagt caaagaggaa aagtatatga gtggcggaga cggacgaggt
     4321 ccgggtaatt caggtctggg acacaatggt ggtcaggcca gtgggaatgt gaacggtaca
     4381 tcaggtaaag gtggcccttc atcaggtggt ggtacggatc caaacagcgg gccgggctgg
     4441 ggtacgacgc atacacctaa cggggatatt cataactata acccggggga gtttggtaac
     4501 ggcgggagta aacccggtgg taatggcggt aacagcggca atcatagcgg gagctctggt
     4561 ggcggacagt cttcggccac cgcgatggcc ttcggtctgc ctgccttggc cactccgggg
     4621 gctgaaggac tggctttatc cgtttccggc gatgcgttgt cggccgctgt tgctgatgtg
     4681 ctggctgccc tgaaaggtcc gtttaagttt ggtctgtggg ggattgcgat ttacggagtg
     4741 ctgccttctg agatagcaaa agatgatccg aaaatgatgt caaaaatcgt gacgtcattg
     4801 ccggcagaca cggtaacgga gacgccggtc agctccctgc cgctggacca ggcgacggtc
     4861 agcgtcacta aacgtgtggc ggatattgtg aaggacgagc ggcagcatat tgcggttgtc
     4921 accggccggc caatgagtgt tcctgtggtg gatgcgaaac cgacaaaacg tccgggggta
     4981 ttcagtgtgt cgattccggg tctcccgtct ctgcaggtga gcgtacctaa aggtgttccg
     5041 gccgcgaaag ccccgccaaa aggcattatt gctgaaaaag gtgattcacg tccggctggt
     5101 tttacggccg gtggtaactc ccgtgaagcc gttattcgtt tcccgaaaga gagcggacag
     5161 aaaccggtct atgtgtcggt gacggatgtt cttactccgg cacaggtaaa acagcgtctg
     5221 gaggaagaaa agcgtcgcca gcaggcatgg gacgctgctc acccggaaga ggggctgaaa
     5281 agagagtatg ataaagcgaa agctgagctg gatgctgaag ataaaaatat tgcgacctta
     5341 aacagtcgca ttgcatcgac agagaaggcg atccccggtg caagggctgc tgttcaggaa
     5401 gccgataaaa aggtaaaaga ggcagaggca aataaagatg attttgtgac ttataacccg
     5461 cctcatgaat atggctccgg gtggcaggat caggttcgct atcttgataa ggatattcag
     5521 aatcagaatg agaaattaaa agctgcccag acatctttaa acgaaatgaa tgaatcctta
     5581 tccagggata aggctgcgct ttccggggcg atggagagcc ggaaacaaaa ggagaaaaaa
     5641 gcgaaggatg cagaaaataa attaaatgag gaaaagaaaa aacctcgtaa gggaactaaa
     5701 gattacggcc atgattattt tcctgatccc aagactgaag atattaaggg gttgggagag
     5761 ttgaaagagg gtaaacctaa aacccctaaa caaggtggtg gtggtaagcg ggcccgatgg
     5821 tatggtgata aaaagcgtaa aatttatgaa tgggattccc agcacggtga gcttgaaggg
     5881 taccgcgcca gtgatggcga acacctcggg gcattcgatc caaaaacggg taagcaggtt
     5941 aaagggccgg atccaaaacg aaacattaaa aaatatcttt aagaggtaaa tatggggctt
     6001 aaattacata ttcattggtt tgataagaaa accgaagagt ttaaaggtgg tgaatactca
     6061 aaagacttcg gtgatgatgg ttctgtcatt gaaagtctgg ggatgccttt aaaggataat
     6121 attaataatg gttggtttga tgttgaaaaa tcatgggttt cgatattaca gccacacttt
     6181 aaaaatgtaa tcgatattag taaatttgat tactttgtat cctttgttta ccgggatggt
     6241 aactggtaag caaaagttat caaggatgat ttgaaataca gacttccggt gttcagggat
     6301 gaataccggg gtttttaaat acagggaagg tatatgaaaa aggtaaaggt tgtttttctt
     6361 tttatattga ttttttcagg ctttcttctg gtcgcatgtc aggcaaacta tatccgggat
     6421 gttcagggtg gaacggtggc accatcgtcc tcctctgaac tgacggggat cgcggttcag
     6481 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc ttttgccctg
     6541 taaacgaaaa aaccacctgg ggaggtggtt tgatcgaagg ttaagtcagt tggggaactg
     6601 cttaaccgtg gtaactggct ttcgcagagc acagcaacca aatctgtcct tccagtgtag
     6661 ccggactttg gcgcacactt caagagcaac cgcgtgttta gctaaacaaa tcctctgcga
     6721 actcccagtt accaatggct gctgccagtg gcgttttgcg tgcttttccg ggttggactc
     6781 aagtgaacag ttaccggata aggcgcagca gtcgggctga acggggggtt cttgcttaca
     6841 gcccagcttg gagcgaacga cctacaccga gccgagatac cagagtgtga gctatgagaa
     6901 agcgccacac ttcccgcaag ggagaaaggc ggaacaggta tccgggaaac ggcagggttg
     6961 gaacaggaga gcgcaagagg gagcgattca tcggaaacgg tgatgatctt taagtcctgt
     7021 cgggtttcgc cactcctgac tgattcatgg ttgagccacc ggctcccaca gatgcaccga
     7081 aaaagcgtct gtttatgtga actcagtcag gagggcggag cctatggaaa aacgccacct
     7141 gcgtggcctt gccgttttgc ctcacatgtt agtcccctgc ttattcacgg aatctgtggg
     7201 taactttgca tgtgtccgca gcgcccgccg cagtctcacg gccggagcgt agcgaccgag
     7261 tgagcgagga ggcgcaattt ccccggcacc tacaagacgg acaccggacg cgctgatgtg
     7321 gcatattgtc ccctgattaa gtctttatat tccctgataa taaattacca ggataggtga
     7381 atcatatcgg tttttcgctg aacattttac gttaacctat ctctgttgct tttttctcct
     7441 tacggagaaa tacaggtcat ccccgcgagg gcgtggctga cctcaggggg atatcccctg
     7501 cttacgctgg ctggacgcca ccgacgcacg gtctctcccc ctctttctgg ctccgtccgg
     7561 ctggacgccg gccacgccag aaattcaccc ggcccccgtc aggctcacgc ctgaccaccc
     7621 acaaaaatcc acggtgctgg acgcacctgg cgaagcagca aagctgctcc tgattttgcg
     7681 ggtggtcagc cgctcgctat ggcgaactct gacgggggcc ggaacaccgg aaaagaccgc
     7741 actggggtgc cgccttttcc tctcacacgc tggggcgtgc tcgttgttcc tccggggctg
     7801 cgccttttcc gcatggggcg gaaagaggct ggcctccgtg gtgaaagctg aacgcaatca
     7861 ccacggacgt gaccgccgcc gccatgcctt ggggcatgtc cgcggcttgt gcagcggggg
     7921 ctgcacatca cgccggcaaa ccatcgtctg aaggagagag aacatgttta atacggattc
     7981 gcttgcctgg aagtggcaca ttccggagct ggcccggcag atttatccgt gggtcagctg
     8041 gctgtaccag gagtattacg gtctggccgg ggctttccta gttatcggcc tgtttttcgg
     8101 tctggctgaa ctttgggccg gacggatgcg ttttgtgggg ctgagtcata aggagcgacg
     8161 ggagtatgcc gatcgctata agcgcgataa taaacggcca gcgccatttt cggtgctggc
     8221 ctcgctggat tcgcccgggc ggatggtcag ggcacgtcga catgtctgga actacgcgta
     8281 ctggctgacc ggcgcggccg gtctggccct tctggggggg ctggttgtcg ccgtgtttat
     8341 cgagagctgg ggagaaaccg tcctgcgtcc ggacctctac gcaaacgcag attattacct
     8401 gctgcagccg gctaacctgt tcgggattgc ctggtgcgcg ggcgggctgg ctgtcgggat
     8461 tgtgttggcg cggctgctgg cgttcctgct gcttgacggt cattttgcgg ctgctgacga
     8521 ggcggtgaac gcgaaactga atcaggagag ccggcgcagc agccagcgga caggagagat
     8581 gaccgacgtg cgccacctgc atttcgggga gccggtaccg gttaatgcgc tggccgattt
     8641 ttcctcggag caggcccggc aggagcaggc ggtgtttctg ggtaaggatg agcagggcaa
     8701 gccggtgctg gttccgcgtg atacctggcg caagaccaac attcagatcc tcggcctgcc
     8761 cgggagcggc aagagcgtga tggccaccaa cgcgctcatt cgctgcgtac gcgatttcgg
     8821 ggacgcggtg gtgtacttcg accccaaagg ggacgcatgg gcgccgcacg tcttccgggc
     8881 gcactgtccg gacttcaccc tgctggatct gcggccgggg aaaccggcgc agctgaatct
     8941 gttccgcgat ctcgaccagt acgcgctgaa aaacctgctg attgccgggc ttaacctggg
     9001 cgagaccggc gacgtggcgg accactaccg catcagcgag cagaaagcgg cgaagctgat
     9061 tgcggagcag tttccgcagg gggcaaacat tcagcaggtg ctggcggcgg cgtacgcgct
     9121 gccggaggcc ctgaaaaagg acgtgaaggg gttgatcact aggctggaaa acgtcgctga
     9181 cctgagcgtc ctgcagacgg acagtggcat cgatgtggcg gggattatca acggcggcgg
     9241 ctgcctgtac gttatcggct cgatggacga cgaggcggtg atccgtgtgc aaaa
//
