LOCUS       NZ_CP069698             2080 bp    DNA     circular CON 18-JUN-2023
DEFINITION  Escherichia coli H20 strain MIN6 plasmid pMUB-MIN6-5, complete
            sequence.
ACCESSION   NZ_CP069698
VERSION     NZ_CP069698.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN17831481
            Assembly: GCF_017570685.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli H20
  ORGANISM  Escherichia coli H20
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2080)
  AUTHORS   Majewski,P.
  TITLE     Plasmid mediated mcr-1.1 colistin-resistance in clinical
            extraintestinal Escherichia coli strains isolated in Poland
  JOURNAL   Front Microbiol (2021) In press
REFERENCE   2  (bases 1 to 2080)
  AUTHORS   Majewski,P.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2021) Department of Microbiological Diagnostics
            and Infectious Immunology, Medical University of Bialystok,
            Waszyngtona 15A, Bialystok 15-269, Poland
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: Unicycler hybrid assembly v. v0.4.8-beta
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 446x
            Sequencing Technology :: Illumina MiSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 06/18/2023 02:24:52
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,084
            CDSs (total)                      :: 4,968
            Genes (coding)                    :: 4,731
            CDSs (with protein)               :: 4,731
            Genes (RNA)                       :: 116
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 101 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 47 of 237
            Pseudo Genes (multiple problems)  :: 43 of 237
            Pseudo Genes (short protein)      :: 2 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP069698.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2080
                     /organism="Escherichia coli H20"
                     /mol_type="genomic DNA"
                     /strain="MIN6"
                     /serotype="H20"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:2810409"
                     /plasmid="pMUB-MIN6-5"
                     /geo_loc_name="Poland: Bialystok"
                     /lat_lon="53.12523570 N 23.15905989 E"
                     /collection_date="2017-09-06"
                     /genotype="ST-553"
     gene            complement(join(1913..2080,1..84))
                     /locus_tag="JSU10_RS25100"
                     /old_locus_tag="JSU10_25095"
     CDS             complement(join(1913..2080,1..84))
                     /locus_tag="JSU10_RS25100"
                     /old_locus_tag="JSU10_25095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001565860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001230707.1"
                     /translation="MRKNKGDVTYFLEKEGDNYRLTKRIKARTNVKIGNKTTKITLYDA
                     VLNENELQHIDFTCAGLREDDETPVKNLIKEFMLNETR"
                     /name="hypothetical protein"
     gene            complement(81..623)
                     /locus_tag="JSU10_RS25105"
                     /old_locus_tag="JSU10_25100"
     CDS             complement(81..623)
                     /locus_tag="JSU10_RS25105"
                     /old_locus_tag="JSU10_25100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001401654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001083906.1"
                     /translation="MNSFFEQYHPVFEVVCRILGNGWRVNKLDDCSSRIKLTSPQFKNY
                     SVHIRMEKDRFSVVGSVDSRSWRSPHHVCTLSRKRNPVDIAADIERKILVNASQEVLQA
                     IEYEKHQVEKKDEILILKGMLSQLVQLESWYGALTGFKAENGLNGKVTEQGDSYDLQIR
                     GLSIDQLVKITGYLKQL"
                     /name="hypothetical protein"
     gene            complement(728..1243)
                     /locus_tag="JSU10_RS25110"
                     /old_locus_tag="JSU10_25105"
     CDS             complement(728..1243)
                     /locus_tag="JSU10_RS25110"
                     /old_locus_tag="JSU10_25105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025391.1"
                     /GO_process="GO:0006457 - protein folding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="WP_001025395.1"
                     /translation="MNIQEALNVFGLSGELTEKDIKAAYRKAALKYHPDRNPLGAELMK
                     AVNAAFDVLMANIDKINQFQSTDEHARYNYGDDLEKVLNVLSGLSGLVFEVIGNWVWIS
                     GETITHKETLKEIGCKWAAKKKQWFYRPDEHKSYWNREEHTIEEIRAKYGTTGQRRATG
                     WQRVETRA"
                     /name="J domain-containing protein"
     gene            complement(1699..1926)
                     /locus_tag="JSU10_RS25115"
                     /old_locus_tag="JSU10_25110"
     CDS             complement(1699..1926)
                     /locus_tag="JSU10_RS25115"
                     /old_locus_tag="JSU10_25110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000801440.1"
                     /translation="MKPDNTPENVKKLRLKAGLTQKECSHIYGVGLRTWQKKEEVNTQN
                     SQSLSLVEFEFLLLLAGEHPEYVLCKRESK"
                     /name="hypothetical protein"
ORIGIN
        1 tctggctttt atcctttttg taagcctgta attgtcacct tctttttcaa gaaaataagt
       61 cacatcacct ttattttttc tcataactgt ttcaaatacc ctgtaatttt aacaagttga
      121 tctatactca agccccgaat ctgtaaatca taactgtcgc cctgttcggt gactttaccg
      181 ttcaatccgt tttcagcttt aaagcctgtc aatgccccat accagctttc aagctggaca
      241 agttgcgata acataccttt caggatcaga atttcgtcct ttttttccac ctggtgtttc
      301 tcatactcaa tcgcctgtaa cacctcctgc gaagcattta ccagaatttt tcgctcgata
      361 tcggctgcaa tatcaacggg attccgtttt ctggataacg tgcaaacatg atgcggacta
      421 cgccaggaac gactatccac actccccacc acagaaaagc ggtctttttc catccgaata
      481 tgcacagagt aatttttaaa ctgcggtgac gtcagtttta tacgagacga acaatcatca
      541 agtttgttca cgcgccagcc gttccccagg atacggcaga caacttcaaa gacagggtga
      601 tattgttcaa aaaaagagtt catctgtttc cctttttgct acgtggctgc tgttcccgtc
      661 tttttgggtt cgatgcccca aaagacgggc tggctataat atcgggggct tctcgccccc
      721 gttctggtta cgctctggtt tccacgcgtt gccaccctgt cgccctgcgc tgtccggttg
      781 taccgtattt tgcgcggatt tcttctatcg tgtgttcttc acgattccag taacttttat
      841 gttcgtctgg acgataaaac cattgttttt tctttgccgc ccatttacac ccgatttctt
      901 ttaaagtttc cttatgtgta atggtttctc cactaatcca gacccagtta cctatcactt
      961 caaataccag accagataaa ccagaaagaa cgtttaatac tttttccagg tcatcaccgt
     1021 aattatatcg tgcatgttca tcagtgctct ggaactgatt tattttatca atatttgcca
     1081 tcaatacatc aaaagctgca tttaccgctt tcatcagttc agcccctaac gggttacgat
     1141 ctggatgata ttttaaagcg gcttttctgt atgctgcttt gatatctttt tcagttaatt
     1201 cgccggataa tccaaaaacg tttaatgctt cctggatatt cattttttta atctcctgcc
     1261 ctgctgggct attgtgagag gggccattcc cctttcgatg aaacaataat acgaccttta
     1321 agtcgtattt gcaagttttc tcccgttttt ttactcgata agtgattgtt atcacgtttt
     1381 ttaatccgcg taagcggatg gaaggggcga acgaaaatac cagggcagca caggagccac
     1441 atcgccccga agcgcggcgc agccgctgac gaacgtacca gggtgcacag gccatccctt
     1501 caccctgcaa agcccaactg accagcaggc catgattatg ctgatttgcg ccgcagaatg
     1561 ccgcagttag cgcgagcgca agcgagttta ccccgccctg gtgcattgca gcatggcggg
     1621 gcgctgtact gcgcgtgtga gcgtcagcga gctacgagcg caactccagg tactaatata
     1681 aaaccatcac aggtcatttt attttgactc ccttttgcag agcacgtatt caggatgttc
     1741 tcctgcaagt aacaaaagaa actcaaactc aaccagtgac agactttgac tgttttgagt
     1801 attaacttcc tcttttttct gccaggttct tagcccaaca ccataaatat gactgcattc
     1861 cttttgtgtt aatcctgctt ttaaacgtaa tttttttaca ttttcaggtg tattatcggg
     1921 tttcatttaa cataaactcc ttaattaaat tcttaactgg cgtctcatcg tcttccctta
     1981 atccggcaca cgtaaaatca atatgttgta attcattctc attcagtaca gcatcataca
     2041 atgtaatttt cgtagttttg ttcccgattt taacatttgt
//
