LOCUS       NZ_CP069697             5631 bp    DNA     circular CON 18-JUN-2023
DEFINITION  Escherichia coli H20 strain MIN6 plasmid pMUB-MIN6-4, complete
            sequence.
ACCESSION   NZ_CP069697
VERSION     NZ_CP069697.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN17831481
            Assembly: GCF_017570685.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli H20
  ORGANISM  Escherichia coli H20
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5631)
  AUTHORS   Majewski,P.
  TITLE     Plasmid mediated mcr-1.1 colistin-resistance in clinical
            extraintestinal Escherichia coli strains isolated in Poland
  JOURNAL   Front Microbiol (2021) In press
REFERENCE   2  (bases 1 to 5631)
  AUTHORS   Majewski,P.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2021) Department of Microbiological Diagnostics
            and Infectious Immunology, Medical University of Bialystok,
            Waszyngtona 15A, Bialystok 15-269, Poland
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: Unicycler hybrid assembly v. v0.4.8-beta
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 446x
            Sequencing Technology :: Illumina MiSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 06/18/2023 02:24:52
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,084
            CDSs (total)                      :: 4,968
            Genes (coding)                    :: 4,731
            CDSs (with protein)               :: 4,731
            Genes (RNA)                       :: 116
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 101 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 47 of 237
            Pseudo Genes (multiple problems)  :: 43 of 237
            Pseudo Genes (short protein)      :: 2 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP069697.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5631
                     /organism="Escherichia coli H20"
                     /mol_type="genomic DNA"
                     /strain="MIN6"
                     /serotype="H20"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:2810409"
                     /plasmid="pMUB-MIN6-4"
                     /geo_loc_name="Poland: Bialystok"
                     /lat_lon="53.12523570 N 23.15905989 E"
                     /collection_date="2017-09-06"
                     /genotype="ST-553"
     gene            211..1263
                     /locus_tag="JSU10_RS25065"
                     /old_locus_tag="JSU10_25060"
     CDS             211..1263
                     /locus_tag="JSU10_RS25065"
                     /old_locus_tag="JSU10_25060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001557502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001053268.1"
                     /translation="MNLQQRINKLPQLSSSFSFGKDIDNIHSFIFNETSKDKIEDLLRK
                     WVSGNQPCVFGKLASKKIKGLDFHLSIVNSPQLYNDDGHLFDFLRNERVRFKERARRGE
                     VSAHLIYFIHPQLAFARPSEELVDIQKYICSLHMPECYPIKEDVIYTESVPFQDKDGLK
                     IYKAGVNVFYSSAHRTRNHDRRIPGGILISVNAPGHFMRLAIEKGFYKDQEQALADIRN
                     MTIQSVGKGGYSHPEGISTTWHSESNLDRFGCPVHTGNSSYYSGFYHTDVLIPGELTKD
                     ERLLHEIENSDPMIFNWNVLFYVSLEEFPIDDPYYGEFIGIPVDDASMFFNSFQPRKFE
                     NKPLYEKEDD"
                     /name="hypothetical protein"
     gene            1265..2131
                     /locus_tag="JSU10_RS25070"
                     /old_locus_tag="JSU10_25065"
     CDS             1265..2131
                     /locus_tag="JSU10_RS25070"
                     /old_locus_tag="JSU10_25065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001335763.1"
                     /GO_function="GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="WP_001335763.1"
                     /translation="MIKGMLFAFLAAFSWGAAIVMSKKGVEELDAGGVFFWQICSAVIL
                     SWIVLFIKRKKLPLNSKAILAYATGVFEPFLAYTFTLYGLYFISAGSASIIFSLESVFI
                     LVLSVLICSVKINSPQYFILFLIGAIVGSILVALPDISNGSNNIVGYCLVVAGVLSAAF
                     YVVISSRLIKSFEPITLLSGQLTFSLILSSLFIFISKSSIYLPLESVIIVFSSGILQYF
                     LAFCFYLNSLRWLPVHLAGIMLYFIPLVALFLSWLFLDEQFTFLQGVGFIVTVLSVYFL
                     NRKYSEE"
                     /name="DMT family transporter"
     gene            complement(3334..3525)
                     /locus_tag="JSU10_RS25075"
                     /old_locus_tag="JSU10_25070"
     CDS             complement(3334..3525)
                     /locus_tag="JSU10_RS25075"
                     /old_locus_tag="JSU10_25070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165975.1"
                     /translation="MTKQEKTALNMARFIRGQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENR"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            3590..3913
                     /locus_tag="JSU10_RS25080"
                     /old_locus_tag="JSU10_25075"
     CDS             3590..3913
                     /locus_tag="JSU10_RS25080"
                     /old_locus_tag="JSU10_25075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_000955999.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGKLPTL
                     APPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVVAALMAIGDELRRLRLAVREQGAR
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            4122..>4553
                     /locus_tag="JSU10_RS25530"
                     /pseudo=""
     CDS             4122..>4553
                     /locus_tag="JSU10_RS25530"
                     /inference="COORDINATES: protein motif:HMM:NF015397.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            4591..5121
                     /locus_tag="JSU10_RS25090"
                     /old_locus_tag="JSU10_25085"
     CDS             4591..5121
                     /locus_tag="JSU10_RS25090"
                     /old_locus_tag="JSU10_25085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001673158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_001673158.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTEKMLNDEFRKLEESVNRELTSNE
                     SLIRNAINDHTTALKELLERYQKTTVDTMDAHWKTVLKMSVKRWLWLIIVSVLMFATTG
                     SLLWYQGMKINANMNILREQKESLEKLNAKTWGVRYHEDSNGRFLVLPKGMKAETNWTK
                     DNGKLNAVRLVQE"
                     /name="MbeB family mobilization protein"
     gene            5128..5361
                     /locus_tag="JSU10_RS25095"
                     /old_locus_tag="JSU10_25090"
     CDS             5128..5361
                     /locus_tag="JSU10_RS25095"
                     /old_locus_tag="JSU10_25090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137437.1"
                     /translation="MTELERHLLNALEQLQQDYMQRLNEWENAFVELQKMFSLTQRDNA
                     MLNGRVMQLSQQVQHLSERTERLSQLYSENRR"
                     /name="MbeD family mobilization/exclusion protein"
     Typing          3159..3240
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="1.3400000000000002e-35"
     Typing          2515..2702
                     /name="rep_cluster_2401"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="4.63e-94"
     Typing          3904..5465
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 aacgcgtttt ggcggtgcgt acaatgcgac gttatggtaa atggagcgga ttttcaggaa
       61 ggagtgatac taacaactcg aacactgtgc aaataatgat ttttttaaaa tatcggtaac
      121 gataataaat tatttcgttt tacgccggta ataaatataa tgtagtttca gtatataaaa
      181 aaatatatga ggtgtcttgt gggagctttc atgaatcttc aacaacgtat aaataaacta
      241 ccacaattat catccagttt ttcatttgga aaagatattg ataatattca ttcatttatc
      301 tttaacgaaa cttctaaaga taaaattgaa gatttactaa gaaaatgggt gtcaggcaat
      361 cagccctgtg tatttggtaa attggcaagc aagaaaatta aagggcttga tttccattta
      421 tctattgtaa atagccccca actatataat gatgatggtc atttgtttga ttttttgcgt
      481 aatgaaagag ttcgttttaa agaacgcgca agaagaggag aggtcagtgc gcatctcatt
      541 tattttattc atccacaact tgcctttgct cgtccatcag aagagttagt ggacatacaa
      601 aaatatattt gttcgctgca tatgccagag tgctatccaa taaaagagga tgttatttat
      661 actgaaagcg taccatttca ggataaagat gggctaaaaa tatataaggc tggtgttaat
      721 gttttttact cttctgctca tagaacaaga aatcatgata gaagaatacc agggggaata
      781 cttatttctg taaatgcccc ggggcatttt atgaggctgg cgatagaaaa aggtttctat
      841 aaagatcaag aacaagcgtt agcggatatt cgtaacatga ctattcagtc tgttggtaag
      901 ggtgggtatt cacatccgga agggatttct acaacatggc attcagaatc taatttggat
      961 cgttttggat gcccagtgca caccggtaat agttcttatt acagcggttt ttatcatact
     1021 gatgtattaa ttccaggaga attaactaag gatgaaagat tgctccatga aatagaaaat
     1081 agtgatccaa tgatcttcaa ctggaatgta ttgttttatg tttcattaga agagtttcct
     1141 atagatgatc cttattatgg agagtttatt ggtattcctg tagatgatgc atctatgttt
     1201 tttaactcct ttcaaccaag gaaatttgaa aacaaacccc tttatgagaa agaggatgat
     1261 taatatgatt aaaggaatgc tttttgcttt tttagcagcg ttctcatggg gggcagctat
     1321 cgtaatgagc aaaaaaggag ttgaagaact agatgccgga ggggtgtttt tctggcagat
     1381 ttgtagtgca gtaatactat cttggatagt tctttttata aaaagaaaaa aactcccatt
     1441 aaactcaaaa gcgatacttg cttatgcgac aggcgttttt gaaccattct tagcgtatac
     1501 ttttacactc tacggacttt attttatttc agcaggaagc gcatcaatta ttttttcact
     1561 tgaatcagtg tttatattgg tgttgtctgt tcttatttgc tcagttaaaa ttaattcccc
     1621 gcaatatttt atattgttcc ttataggtgc aattgttggc agtattttgg tagccttgcc
     1681 agacatcagt aatggatcga ataatattgt tggatattgt cttgttgttg ccggtgtgtt
     1741 atcggcagca ttttatgttg ttatatcgtc tcgccttatt aaatcgtttg agcctatcac
     1801 attattgagc ggacagttga cattctccct tatactgtca tcgctcttta tttttatatc
     1861 taaatcatca atttatcttc cgttggaatc cgtgattatt gttttttcat ctggaatatt
     1921 acaatatttt ttagcttttt gtttttactt gaactccttg cgttggctcc cagttcattt
     1981 ggcagggata atgttgtact ttattccgct agttgcatta tttttgtcat ggttgttttt
     2041 ggatgaacaa ttcacattct tacagggggt aggatttatt gtaactgtgc taagtgtata
     2101 ttttcttaac agaaaatact cagaggagta acattctgtt caggattgcc ccccggcctc
     2161 catcgatacc gtgccaaatg cacgtaaaaa acgggagcgt ttctggtgtc ggaatgtaac
     2221 caagggggtc agaggcgcgc taaaacgctc tcaggggagt caaatagaga cgtttgtaac
     2281 aaaaagatca aaggatcctc ttgagatcct ttttttctgc gcgtaatctg ctgcctgtaa
     2341 acgaaaaaac cgccttgcag ggcggtttga tcgaaggtta agtcagcttg cagactgctt
     2401 aaccaggtaa ctgtcttatg cgagacgagt caccaaaatt gttcttctag tgtagccgca
     2461 ctccggcagc cacttcaagt actctctaaa tatctcgcac atgactttta ccaatggctg
     2521 ctgccagtgg cgctttgtgg tgtcttttcc gggttggacc caagagaaca gttaccggag
     2581 aaggcgcagc agtcgggctg aacggggggt tcctgcatac agcccagccc ggagcgaact
     2641 gcctaagtcg ccccgagata ccagaagacg tgagctatga gaaagcgcca cgtgtcccgt
     2701 aagggagaaa ggcggaaaag gtatccggta atcgatatgg cgggaacgtg ggggcgcagg
     2761 agggagcgat tcaccggaaa cggtgatgat ctttaagcca cgtcgggttt cgccaccgct
     2821 ggcggatttc atggttgagc cgcaaggctc ccacagatgc accgaaaaag tgtctgtttt
     2881 gtgaagtccg tcaggggggc ggagcctatg gaaaaagcgc ctccggcgcg gcctttcccg
     2941 gggtttcgtt ttttgcgcac atgttctttc ctggtttatc cccgggttct gtggataaca
     3001 gggttagcgt ttttgagaaa ggagagtccg ctcgccgcag ccgaacgacc gagcgtagcg
     3061 agtcagtgag cgaggaagcg gaagagcgcc tgaactggca ttttctcctt acgcatctgt
     3121 gcggcatttc acaccggaca tggcgcactt ttcatacaat ccgcactgat gccgaatggt
     3181 taagccagta tacactccgc tatcgctacg tgactgggtc agggcttcgc cccgacaccc
     3241 caaaggtcaa cggcctgacg cgccctggcg ggcttgtctg ctcccggcat ccgctcacag
     3301 acaagctgtg accgtctccg ggagctgcat gtgtcagcgg ttttcaccgt catccccgaa
     3361 gcgtgcgagg caactgcggt aaagctcatc ggcgtggtcg tgaagcgatt cacagatgtc
     3421 tgcctgttca tccgcgtcca gttcgttgag cttctccagc agcgttaatg tctggcctct
     3481 gatgaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta
     3541 agggggggtt ctgttcatgg gggtaatgat accgatgaaa cgcgagagga tgctcacgat
     3601 acgggttact gatgatgaac atgcccggtt actggaacgc tgtgagggta aacaactggc
     3661 ggtatggatg cggcgggtct gcctgggtga gccggttgcc cgttccggga agctgccgac
     3721 actggcaccg ccgttactgc gtcagctggc cgccatcggg aataacctta accagacagc
     3781 gaggaaggtg aacagcgggc agtggtcttc cggtgaccgg gttcaggtgg tggccgcact
     3841 gatggccatc ggggatgagc tgcgccggct gcgtctggcg gtcagggaac agggggcgcg
     3901 ggatgatagt taagtttcat gccaggggaa aaggtggtgg cagtggtccg gttgattacc
     3961 tgctgggaag ggagcgtaac cgggaagggg caacggtgct tcaggggaat ccggaagagg
     4021 tccgggaact catcgatgcc acgccatttg cgaagaaata cacgtcaggt gttctgtcgt
     4081 tcgcggagaa ggagctgccg cctggagggc gtgaaaaagt gatggcgagc tttgagcgtg
     4141 tactgatgcc cggtctcgag aaaaatcagt acagcatcct gtgggtggag caccaggaca
     4201 agggacggct tgagctgaat tttgtcattc cgaacatgga gctacagacc ggaaaacgcc
     4261 tccagccgta ctacgaccgg gcagacaggc cgagaattga tgcctggcag acgctggtga
     4321 atcaccatta cggtctgcat gaccccaacg ccccggagaa ccgccggacg ctgacccttc
     4381 ctgataacct gccggaaacg aaacaggcgc ttgctgaggg cgtcacacgc gggatagacg
     4441 cgctttacca tgccggagag ataaaaggcc gtcaggatgt gattcaggcg ctcacagagg
     4501 cggggcttga agtggtcagg gtgacgcgaa gcagtatcag cattgcagac ccgaacggcg
     4561 ggaggaatat cagactgaaa ggagcgtttt atgagcaatc ttttacagac gggcgcggag
     4621 ttcgagaaaa agctgaaaga gagagccgaa tctacagaga aaatgctgaa cgacgagttc
     4681 aggaagctcg aagaatctgt aaacagggaa ctgacctcaa acgaatcttt aatcaggaac
     4741 gctatcaacg accatacaac agccctgaaa gaactgctgg agaggtatca gaaaacgacc
     4801 gtcgatacga tggacgcgca ctggaaaacc gtactgaaaa tgagcgtgaa acgctggctg
     4861 tggctgatta tcgtctcagt actgatgttt gccacgaccg ggagtctgct gtggtaccag
     4921 ggcatgaaga taaacgcgaa tatgaatatt ctccgcgagc agaaagagag tctggagaag
     4981 ctgaacgcga agacatgggg cgtgcgttat cacgaggaca gcaacggacg ttttctggtg
     5041 ttgccgaagg ggatgaaagc cgaaacgaac tggacgaagg acaacggcaa actgaacgca
     5101 gtgagactgg tgcaggagtg acagagcatg acggagctgg aaagacattt gctgaacgca
     5161 ttagagcaac tgcaacagga ctatatgcag cggctgaacg aatgggagaa cgccttcgtg
     5221 gaattgcaga aaatgttttc gcttacgcaa cgggacaacg cgatgctgaa cgggcgagtc
     5281 atgcagttga gtcagcaggt gcagcacttg agcgagcgga cagaacgctt gagccagtta
     5341 tacagcgaga acaggagata agggatgaac ggctgatgca ggaacgcgaa catgagttat
     5401 ctctggagcg ggagcgtcag ccggaaatcc aggagcggac actggatggc ccttcgctgg
     5461 gatggtgaaa ccatgaaaaa tgacgtgttc agtggattaa ggcgggctaa tgtggcctgt
     5521 agcatctggt tgcataggta ttcatgcggt taaaatttat caggtgcgat cgcggcagtt
     5581 tttcgggtgg tttgttgcca tttttacctg tctgctgccg tgatcgcacc g
//
