LOCUS       NZ_CP069696             6293 bp    DNA     circular CON 18-JUN-2023
DEFINITION  Escherichia coli H20 strain MIN6 plasmid pMUB-MIN6-3, complete
            sequence.
ACCESSION   NZ_CP069696
VERSION     NZ_CP069696.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN17831481
            Assembly: GCF_017570685.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli H20
  ORGANISM  Escherichia coli H20
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6293)
  AUTHORS   Majewski,P.
  TITLE     Plasmid mediated mcr-1.1 colistin-resistance in clinical
            extraintestinal Escherichia coli strains isolated in Poland
  JOURNAL   Front Microbiol (2021) In press
REFERENCE   2  (bases 1 to 6293)
  AUTHORS   Majewski,P.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2021) Department of Microbiological Diagnostics
            and Infectious Immunology, Medical University of Bialystok,
            Waszyngtona 15A, Bialystok 15-269, Poland
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: Unicycler hybrid assembly v. v0.4.8-beta
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 446x
            Sequencing Technology :: Illumina MiSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 06/18/2023 02:24:52
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,084
            CDSs (total)                      :: 4,968
            Genes (coding)                    :: 4,731
            CDSs (with protein)               :: 4,731
            Genes (RNA)                       :: 116
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 101 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 47 of 237
            Pseudo Genes (multiple problems)  :: 43 of 237
            Pseudo Genes (short protein)      :: 2 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP069696.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..6293
                     /organism="Escherichia coli H20"
                     /mol_type="genomic DNA"
                     /strain="MIN6"
                     /serotype="H20"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:2810409"
                     /plasmid="pMUB-MIN6-3"
                     /geo_loc_name="Poland: Bialystok"
                     /lat_lon="53.12523570 N 23.15905989 E"
                     /collection_date="2017-09-06"
                     /genotype="ST-553"
     gene            complement(777..1514)
                     /locus_tag="JSU10_RS25025"
                     /old_locus_tag="JSU10_25020"
     CDS             complement(777..1514)
                     /locus_tag="JSU10_RS25025"
                     /old_locus_tag="JSU10_25020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013934312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-2 restriction enzyme EcoRV"
                     /protein_id="WP_011117659.1"
                     /translation="MSLRSDLINALYDENQKYDVCGIISAEGKIYPLGSDTKVLSTIFE
                     LFSRPIINKIAEKHGYIVEEPKQQNHYPDFTLYKPSEPNKKIAIDIKTTYTNKENEKIK
                     FTLGGYTSFIRNNTKNIVYPFDQYIAHWIIGYVYTRVATRKSSLKTYNINELNEIPKPY
                     KGVKVFLQDKWVIAGDLAGSGNTTNIGSIHAHYKDFVEGKGIFDSEDEFLDYWRNYERT
                     SQLRNDKYNNISEYRNWIYRGRK"
                     /name="type-2 restriction enzyme EcoRV"
     gene            complement(1498..1725)
                     /locus_tag="JSU10_RS25030"
                     /old_locus_tag="JSU10_25025"
     CDS             complement(1498..1725)
                     /locus_tag="JSU10_RS25030"
                     /old_locus_tag="JSU10_25025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008276392.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="WP_069354220.1"
                     /translation="MSVREIIKKNRVICKLSQGEVAKALGKPQSYISKIEQGERRVDVD
                     EFIAICFAIGIDPINTLKEVIMEKKHESSF"
                     /name="helix-turn-helix transcriptional regulator"
     gene            1821..2717
                     /locus_tag="JSU10_RS25035"
                     /old_locus_tag="JSU10_25030"
     CDS             1821..2717
                     /locus_tag="JSU10_RS25035"
                     /old_locus_tag="JSU10_25030"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dam family site-specific
                     DNA-(adenine-N6)-methyltransferase"
                     /protein_id="WP_001570854.1"
                     /translation="MKDKVFVPPIKSQGIKTKLVPWIKRIVPKNFNGVWVEPFMGTGVV
                     AFNVAPKDALLCDTNPHLISFYNALKNKDITGDLVKDFLYREGEKLLLSNGEYYYEVRE
                     RFNNYKEPLDFLFLNRSCFNGMIRFNSKGGFNVPFCKKPNRFAQAYITKISNQVDRISE
                     IISKGNYTFLCQSFEKTIGMVNRDDVVYCDPPYIGRHVDYFNSWGERDERLLFETLSSL
                     NATFITSTWHHNDYRENKYVRDLWSSFRILTKEHFYHVGASEKNRSPMVEALITNIAKD
                     IIDHIEKSSGDILVIEE"
                     /name="Dam family site-specific
                     DNA-(adenine-N6)-methyltransferase"
     gene            complement(3783..3974)
                     /locus_tag="JSU10_RS25040"
                     /old_locus_tag="JSU10_25035"
     CDS             complement(3783..3974)
                     /locus_tag="JSU10_RS25040"
                     /old_locus_tag="JSU10_25035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165985.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            4039..4362
                     /locus_tag="JSU10_RS25045"
                     /old_locus_tag="JSU10_25040"
     CDS             4039..4362
                     /locus_tag="JSU10_RS25045"
                     /old_locus_tag="JSU10_25040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_069354221.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGKLPTL
                     APPLLRQLAAIGNNLNQTARKVNSGQWSSCDRVQVVAALMAIERELRSLRQVVREQGAR
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            4571..>5002
                     /locus_tag="JSU10_RS25525"
                     /pseudo=""
     CDS             4571..>5002
                     /locus_tag="JSU10_RS25525"
                     /inference="COORDINATES: protein motif:HMM:NF015397.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            5040..5558
                     /locus_tag="JSU10_RS25055"
                     /old_locus_tag="JSU10_25050"
     CDS             5040..5558
                     /locus_tag="JSU10_RS25055"
                     /old_locus_tag="JSU10_25050"
                     /inference="COORDINATES: protein motif:HMM:NF016710.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_015328227.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTEKMLNDEFRKLGESVSEAVTSNE
                     TKIRDAIALFTASTEKSLEKHREGVKEAMMQHRKDVLKLAGNTGMMLLGIVFLLFTASG
                     GTLWYLGGRIQANLEEIRKQEETLQKLNAKTWGVEFVQDGNRKFLVLPYGKSAEVIPFQ
                     GKEWVHLKE"
                     /name="MbeB family mobilization protein"
     gene            5565..5798
                     /locus_tag="JSU10_RS25060"
                     /old_locus_tag="JSU10_25055"
     CDS             5565..5798
                     /locus_tag="JSU10_RS25060"
                     /old_locus_tag="JSU10_25055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_011117658.1"
                     /translation="MTELETHLLNALEQLQQDYMQRLSEWESAFVELQKMFSRTQRDNA
                     MLNERVMQLSQQVQHLSEQTERLSQLYSENWR"
                     /name="MbeD family mobilization/exclusion protein"
     Typing          3612..3699
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="6.86e-39"
     Typing          complement(3044..3588)
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.446"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          4353..5902
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="95.038"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 cttatcctgt attggggttt taaattatac attatttaaa tactaaactc tctaagagtc
       61 ggaggagaag tgatgttgta aataagaata catgaggact gatgccagtg attctaatat
      121 taaactatat caagaaattt cctcaactga tgtattgatt ttttgtaagg gcgcgtttat
      181 gtaaagaggt gtatgaataa caagaagtaa ataaacgtca ttatattaca tataatggtt
      241 ttctatttta ttttattatt gtgttcgttg caataaaaga agctgtttat ataaaaatat
      301 gcgtgtggtt tatgataagc taaaaatact ttttgtaatt aacaaaaatg tgttgatttt
      361 tattaagaat caagttacaa cggacatggg tgcgtttcta gtcgtttatc attgtaaccg
      421 gtctcaatgt gagaagcgca cccaattctt attttccgca atgtatagac atacagaaaa
      481 aattctctct cattggtgtt gaattaaaca tcattcaagt gatatgtata tttattgtgc
      541 gaaatgcaga gggaaaatat agagaggcat atgtttttat gtctttatgt tgttgtggtg
      601 agagtgtaag ggtgcatcat gtcatctttg aatgttaagt gtgatgtgcc ggaggaggga
      661 atagtctgat gctaaatttt ttcttttagt atccctccct ccccctcttt ctctcatgag
      721 tcagagaggt tttattctct gttagagggg ggggatataa acatataagc tattgcttat
      781 tttcttcctc ggtatatcca gtttctgtat tcgcttatat tattatactt gtcatttctt
      841 aattgactgg ttctttcata atttctccaa tagtctaaaa actcatcctc tgagtcaaat
      901 attccttttc cttctacaaa gtctttatag tgggcatgaa tgctacctat atttgttgtg
      961 tttccagatc ctgccaaatc tccagcaata acccatttat cttgtaagaa aacctttacg
     1021 cctttgtatg gtttagggat ttcattgagt tcatttatat tatatgtttt taaagatgat
     1081 tttcttgtag caactcttgt atatacatat ccgattatcc aatgggcgat atattggtca
     1141 aatggataaa caatattttt tgtgttgttt cgtataaagc tggtataccc accaagagtg
     1201 aacttgattt tttcgttttc tttgtttgta tatgttgttt ttatatctat tgcaattttt
     1261 ttatttggtt cgcttggttt gtaaagagta aagtcaggat aatgattttg ttgtttaggt
     1321 tcttctacaa tatacccatg tttttctgct attttattta ttattggtct tgagaataac
     1381 tcaaatattg tgcttagaac ttttgtgtca cttcccaatg ggtatatttt tccttctgca
     1441 gatattattc cgcatacatc atatttttga ttttcatcat atagtgcatt aattaaatca
     1501 gaacgaagac tcatgttttt tttccattat tacttcttta agtgtattta ttgggtcaat
     1561 tcctattgca aaacagatgg ctataaattc gtcaacgtca acgcgtcttt ctccttgttc
     1621 tattttagat atgtaggatt gtggctttcc aagtgccttg gcaacctctc cttgagagag
     1681 tttacaaatt actctatttt tttttatgat ttctcttaca gacatagatt taccatcatt
     1741 ttttttatat attatgcacc ccaaaatggg ataacccaaa atgggtttct ttggcatatt
     1801 ttttacaaat ggttcttatt atgaaagata aagtttttgt tccgcctata aaatcgcaag
     1861 ggattaagac taaactggtt ccgtggataa agagaattgt tcctaaaaac tttaatgggg
     1921 tatgggtcga gccgtttatg gggactggag ttgttgcctt taacgttgct cctaaagatg
     1981 cattattatg tgatacaaac cctcatttga tttcttttta taatgcattg aaaaataaag
     2041 atataacagg ggatcttgtt aaggattttt tatatcgtga aggtgaaaaa ctcttattaa
     2101 gtaatggtga gtattattat gaagtcaggg aaaggtttaa taattacaag gaaccgttag
     2161 attttttatt cttaaatagg tcttgtttta atggtatgat taggtttaat agtaaaggag
     2221 ggtttaatgt tcctttttgt aaaaaaccaa acagatttgc acaagcatat attaccaaga
     2281 tttcgaatca agttgatagg atttctgaaa taatatcaaa agggaactat acattcttgt
     2341 gtcagtcatt tgaaaaaact attggcatgg taaataggga tgatgttgtt tattgtgatc
     2401 ctccatatat tggtaggcat gttgattatt ttaattcatg gggagagcgt gacgagcgtt
     2461 tattatttga aacgttatca tctttgaatg ctacatttat aacgtctacg tggcatcata
     2521 atgattatag ggaaaataag tacgttcgtg atttatggtc atcttttaga atattgacaa
     2581 aagaacattt ttatcatgtt ggagcgtccg aaaaaaacag atccccgatg gtagaggcgc
     2641 taattacaaa tatagccaag gatattattg atcacattga aaaaagtagt ggggatattt
     2701 tggtaattga agagtgatgg agatgaggac ataaagtgac cactctgtac gtttatcgtg
     2761 agaggggata tgtaaaaaga tctcaagatc ctcttgagat cctttttttt cgcgcgtaat
     2821 ctgctgtctg tagacgaaaa aaccaccctg gcaggtggtt tttcgaaggt taaataatcc
     2881 tggcagatta tttaaccgtg gtaacagggt gtacaagacc gctgccacca aatctgtcct
     2941 ttcagtgtag ccgcagttgg tccttcactt caagaactac gtatcagcaa tttcttgtac
     3001 atcctctacc agtggctgct gccagtggcg ttaaggcgtg tcttaccggg ttggactcaa
     3061 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     3121 ccagcttgga gcgaacgacc tacaccgaac tgagatacca acagcgtgag ctatgagaaa
     3181 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     3241 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     3301 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     3361 tatggaaaaa cgcctcctgc gcgccctttt tctgggtctt tgtcttttgc tcacatgttc
     3421 tttccggcgt tatcccctga ttctgtggat aaccgtttac cgcctttgag tgcgctgaca
     3481 ccgctcgccg cagtcgaacg accgagcgta gcgagtcagt gagcgaggaa gcggaagagc
     3541 gcctggacgt gcattttctc cttacgcatc tgtgcggcat ttcacacccg gcatggcgta
     3601 cttttcatac aatccgcact gatgccgcat ggttaagcca gtatacactc cgctatcgct
     3661 acgtgactgg gtcagggctg cgccccgaca cccgctaaaa cctgctgacg cgccctgacg
     3721 ggcttgtctg ctcccggaga cgctcacaga caagctgtga gcgtctccgg gagttgcatg
     3781 tgtcagaggt tttcaccgtc atccccgaaa cgtgcgaggc agctgcggta aagctcatcg
     3841 gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ctgcgtccag ttcgttgagc
     3901 ttctccagca gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt
     3961 tcctgtttag tcactgatgc ctccgtgtaa ggggggattt ctgttcatgg ggtaatgata
     4021 ccgatgaaac gcgagaggat gctcacgata cgggttactg atgatgaaca tgcacggtta
     4081 ctggaacgct gtgagggtaa acagctggcg gtatggatgc ggcgggtctg cctgggtgag
     4141 ccggttgccc gttccgggaa gctgccgaca ctggcaccgc cgttactgcg ccagctggct
     4201 gccatcggga ataacctgaa tcagacggcg aggaaggtga acagcgggca gtggtcttcc
     4261 tgtgaccggg ttcaggtggt ggccgcactg atggccatcg agagagagtt gcgcagctta
     4321 cggcaggtcg tcagggaaca gggggcgcgg gatgatagtt aaatttcatg ccaggggaaa
     4381 aggtggtggc agtggtccgg ttgattacct gctggggagg gagcgtaacc gggaaggggc
     4441 aacggtgctt cagggtaatc cggaagaagt ccgggaactc atcgatgcca cgccatttgc
     4501 gaagaaatac acgtccggtg ttctgtcgtt cgcggagaag gaactgccgc cgggaggacg
     4561 tgaaaaagtg atggcgagct ttgagcgcgt actgatgcca ggtctcgaaa agaatcagta
     4621 cagcatcctg tgggtggagc accaggacaa gggacggctt gagctgaatt ttatcattcc
     4681 gaacatggag ctacagaccg gaaaacgtct ccagccgtac tacgaccggg cagacaggcc
     4741 gagaattgat gcctggcaga cgctggtgaa tcaccattac gggctgcatg accccaacgc
     4801 cccggagaac cgccggatac tgactctctc tgataacctg ccggaaacga aacaggcgct
     4861 tgctgagagc gtcacacgcg ggatagacgc tctttatcat gtaggggaga taaaaggccg
     4921 tcaggatgtg attcaggcgc tcacagaggc ggggcttgaa gtggtcaggg tgacgcgaag
     4981 cagcatcagc attgcagacc cgaacggcgg gaagaatatc aggctgaaag gagcgtttta
     5041 tgagcaatct tttacagacg ggcgcggagt tcgagaaaaa gctgaaagag agagccgaat
     5101 ctacagagaa aatgctgaac gacgagttca ggaagctcgg cgagtctgtc agcgaggctg
     5161 tgacctcaaa cgagacgaaa atcagagacg ctatagccct gttcacagcc tcgacagaga
     5221 aatcgctgga aaaacacagg gaaggggtga aagaggcgat gatgcagcac aggaaggacg
     5281 tgttaaagct ggcagggaat acgggcatga tgttactggg gatagtcttt ctcctgttta
     5341 ccgcgagtgg cgggacgctc tggtatcttg gcgggaggat acaggcgaac ctggaagaaa
     5401 tcaggaagca ggaagagaca ttgcagaaac tgaacgcgaa gacatggggc gtggagtttg
     5461 tgcaggacgg gaacaggaaa tttctggtcc ttccgtacgg gaaatcagca gaggtgattc
     5521 cctttcaggg gaaagagtgg gtacatctga aggagtgaca cagaatgaca gagctggaaa
     5581 cacatttgct gaacgcctta gagcagctgc aacaggacta tatgcagcgg ctgagcgaat
     5641 gggagagcgc cttcgtggaa ttgcagaaga tgttttcgcg tacgcaacgg gacaacgcga
     5701 tgctgaacga gcgggtcatg cagttgagtc agcaggtgca gcacttgagc gagcagacag
     5761 aacgcttgag ccagttatac agcgagaact ggagataaga gaggaacgac tgatacagga
     5821 gcgcgaacat gcgttatccc tggagcgtga gcgccagctg gaaatacagg aacgcacact
     5881 ggatggtcct tcgctgggat ggtgaaacta tgaaaaatgg caggttaagt ggattaagac
     5941 aggctaatgt ggtctgtacc atctggttgc ataggtattc atgcggttaa aatttatcag
     6001 gcgcgattgc ggcagttttt cgggtggttt gttgccattt ttacctgtct gctgctgtga
     6061 tcgcgctgaa cgcgttttgg cggtgcgtac aatttgtaat tatgttaaat ataatagata
     6121 agctcagggt gggggcctat ggcttatatt tgactataaa gctatttatc atttattttc
     6181 catctcgatg cattattaat aaatttcttt tattacaata aaagacgagg atatgattta
     6241 cttttagagt atgtgatttg ataggcaatg ctgttgatat atagcattta atg
//
