LOCUS       NZ_CP069695            33288 bp    DNA     circular CON 18-JUN-2023
DEFINITION  Escherichia coli H20 strain MIN6 plasmid pMUB-MIN6-MCR, complete
            sequence.
ACCESSION   NZ_CP069695
VERSION     NZ_CP069695.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN17831481
            Assembly: GCF_017570685.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli H20
  ORGANISM  Escherichia coli H20
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 33288)
  AUTHORS   Majewski,P.
  TITLE     Plasmid mediated mcr-1.1 colistin-resistance in clinical
            extraintestinal Escherichia coli strains isolated in Poland
  JOURNAL   Front Microbiol (2021) In press
REFERENCE   2  (bases 1 to 33288)
  AUTHORS   Majewski,P.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2021) Department of Microbiological Diagnostics
            and Infectious Immunology, Medical University of Bialystok,
            Waszyngtona 15A, Bialystok 15-269, Poland
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: Unicycler hybrid assembly v. v0.4.8-beta
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 446x
            Sequencing Technology :: Illumina MiSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 06/18/2023 02:24:52
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,084
            CDSs (total)                      :: 4,968
            Genes (coding)                    :: 4,731
            CDSs (with protein)               :: 4,731
            Genes (RNA)                       :: 116
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 101 of 237
            Pseudo Genes (incomplete)         :: 135 of 237
            Pseudo Genes (internal stop)      :: 47 of 237
            Pseudo Genes (multiple problems)  :: 43 of 237
            Pseudo Genes (short protein)      :: 2 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP069695.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..33288
                     /organism="Escherichia coli H20"
                     /mol_type="genomic DNA"
                     /strain="MIN6"
                     /serotype="H20"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:2810409"
                     /plasmid="pMUB-MIN6-MCR"
                     /geo_loc_name="Poland: Bialystok"
                     /lat_lon="53.12523570 N 23.15905989 E"
                     /collection_date="2017-09-06"
                     /genotype="ST-553"
     gene            complement(join(32969..33288,1..397))
                     /locus_tag="JSU10_RS24835"
                     /old_locus_tag="JSU10_24830"
     CDS             complement(join(32969..33288,1..397))
                     /locus_tag="JSU10_RS24835"
                     /old_locus_tag="JSU10_24830"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein"
                     /protein_id="WP_000832283.1"
                     /translation="MKRVKTLMLISLLTASFYSSAGIPVAIDASPEWAVEAARWTERLK
                     QWSETAQHYQSQIQAYKDQLATATGIRNIAAFTNELSNLQSELTNIYKQGNSYISDFTS
                     NPEGALSSQAKNLFSKYGAFDMCNTGYERNDNLCKARIVSTAASIEQGNEINKQLSSAM
                     SQIQSLSSRIEASKDIKESQDLANALQAQSLKMQAIKMQYDVWNNKNKADHEMLVTQEQ
                     EAFIKQQKEAEPLTFD"
                     /name="type IV secretion system protein"
     gene            complement(409..3156)
                     /locus_tag="JSU10_RS24840"
                     /old_locus_tag="JSU10_24835"
     CDS             complement(409..3156)
                     /locus_tag="JSU10_RS24840"
                     /old_locus_tag="JSU10_24835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirB4 family type IV secretion/conjugal transfer
                     ATPase"
                     /protein_id="WP_000105985.1"
                     /translation="MSTLYKAMTRPAMYVGVPVVPLTVVAGALFLAGVYISKLIWLAIP
                     VAVFVLRMITKQDDHIFNLYFLKLKMLGNSVCNRFFGARAFLSGQYEAVEIDEFVNAMK
                     LNERITTGKYIPYSSHVDKNIVKTKNGDYVATWQLMGINFESISAEMLETIDSQVATLV
                     RSFSGLPVSFYNHSCRASFYDAFTTKSGNKYADIISDCYYGSMKKNKFKGNTLYFTLIY
                     RPDGRVEKLEKRKKSIKEKKDDINIHVKRMNEMINTFSGALDKFTCKLLGMYEENGKVF
                     SSQLSFYNYLLTGKLQKIRVTDSPVYNVLGGVDVFFNHDTGQICRIDGNKFFRSIEIKD
                     FCSETASGVFDVLQYSDADYIITHSYTSMSKSEALSTIKRAEKQLKSTEDDAVTQLQEL
                     EKAKNDIVSGDISFGYYHFTLMVMADSIRELDESVSKITADFTDLGIIPALSTMSLPAA
                     YFAQLPAVFHLRPRLSPVSNVNFVELASFHNFYQGKRDKNCWTEAVAILKTPSKQAYYL
                     NLHNSVLFKDETGEKNLANTKVIGTAGSGKTMFLSYLACSLQKYNNPETFADSAKNKKL
                     TCVFLDKDRGAELCIRMLGGEYYTVKSGEPTGWNPFALEATKRNRIFVKQLMEILCTRN
                     GERLSTRERLLISESVDAVMDFPPGEMREYGITRMLEHLMQRDDRDEQENGIILRLSQW
                     ANGQAHGWVFDNAKDTFNIQHVNNFGIDGTEFLDDPMVCAPITFYLLYRITQLLDGRRL
                     VIFLDEFWKWLQDEAFSDFVYNKLKTIRKLNGLVIPATQSPDEILKNKISRAVVEVCST
                     SIYLANPDADYNDYVEGLKLTPEEFNIVKNLDPMSRQFLIKKSSLKKGDGKSFSALATL
                     DLSGLGGYLKILSASADNLEIFESIYHEGMEPDDWVPEYLERAI"
                     /name="VirB4 family type IV secretion/conjugal transfer
                     ATPase"
     gene            complement(3175..3501)
                     /locus_tag="JSU10_RS24845"
                     /old_locus_tag="JSU10_24840"
     CDS             complement(3175..3501)
                     /locus_tag="JSU10_RS24845"
                     /old_locus_tag="JSU10_24840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000008644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrbC/VirB2 family protein"
                     /protein_id="WP_000008647.1"
                     /translation="MSFKKSVCNRISSSVTAVMQRKNKVVMAVAGATVASPAFADGFSK
                     AETLLEKVKTGLSGLSLVTVTIACLWVGYKVLFGGSTIRECSPIIIGAIVIASAAEVAS
                     MMVN"
                     /name="TrbC/VirB2 family protein"
     gene            complement(3562..4134)
                     /locus_tag="JSU10_RS24850"
                     /old_locus_tag="JSU10_24845"
     CDS             complement(3562..4134)
                     /locus_tag="JSU10_RS24850"
                     /old_locus_tag="JSU10_24845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001690148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing protein"
                     /protein_id="WP_227537772.1"
                     /translation="MASLFPALLLCAASVHPDTINDIARVESGFNPYAVAEIIPERERG
                     SYGKSVISHMPKSKEEALTVIREIENRKRRYSVGLMQITSSNFSFYSTSAEKLLDSCEN
                     LSVFEKIIVDCYKRGRSLENALSCYYTGNFSNGKRKEKEFNNTSYVERIGYTGNEKNML
                     YQVQEATGENSEKIVHTMRQLSGPKPF"
                     /name="lytic transglycosylase domain-containing protein"
     gene            complement(4490..4705)
                     /locus_tag="JSU10_RS24855"
                     /old_locus_tag="JSU10_24850"
     CDS             complement(4490..4705)
                     /locus_tag="JSU10_RS24855"
                     /old_locus_tag="JSU10_24850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000760375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EexN family lipoprotein"
                     /protein_id="WP_000760375.1"
                     /translation="MKKYLLFALPFFVVGCSEEVKSVDWWGQHLTEAKQKQAECEKSGS
                     DSQNCKNVKQALFIQSQKDAPVPTFD"
                     /name="EexN family lipoprotein"
     gene            complement(4702..4995)
                     /locus_tag="JSU10_RS24860"
                     /old_locus_tag="JSU10_24855"
     CDS             complement(4702..4995)
                     /locus_tag="JSU10_RS24860"
                     /old_locus_tag="JSU10_24855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000134733.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0003700 - DNA-binding transcription factor activity
                     [Evidence IEA]; GO:0016987 - sigma factor activity
                     [Evidence IEA]"
                     /GO_process="GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]; GO:0006355 - regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="WP_060598542.1"
                     /translation="MTEHDAICISGLHQIFSDEEHLSEQQKDIILMYAYGYTLNEIADF
                     KGLKPSTVRKYLDSVRAELGGVSLAGIRTLVLIRTNALLVSSLSRISERGNL"
                     /name="sigma factor-like helix-turn-helix DNA-binding
                     protein"
     gene            complement(5072..6301)
                     /gene="taxC"
                     /locus_tag="JSU10_RS24865"
                     /old_locus_tag="JSU10_24860"
     CDS             complement(5072..6301)
                     /gene="taxC"
                     /locus_tag="JSU10_RS24865"
                     /old_locus_tag="JSU10_24860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001329504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA relaxase/nickase TaxC"
                     /protein_id="WP_001329504.1"
                     /translation="MGVYVEQEYRIKRAKGTGRDPKSPKLSGRHIHASKSAFNHKVRHG
                     ADKKAYTWTPTKEVTFKITGSGKTAAGIKNGIDYITRNGELEAYCYDGKGSEQTGKGED
                     FNREFTSTLSKGNDYSRTYRGENIDHVKNMVFSPPPEAGVSREDALKATVEFLKETYPN
                     HAFVAVYHDDKEDHPHVHVNIKLRDEETGRRLRLTKSETRKFRNGFHRKLKGMGYDVTA
                     TWKKDPERKREIERLQAENPKRLRNVYKVVDFGETSYQNKAGEKRTPFLTYETLKGGKQ
                     VTIWGKDLKNHFESEKLQPGTLIKVKKLAPTLVRSPMFNDDGTVAGYRETHRNNWQIEN
                     IALERNRERQVHERETRQPEESDVKKQLSRKHEQGHNIGFALEHGFTKDSEEHKKLRIQ
                     QERNWKGLGF"
                     /name="taxC"
     gene            complement(6305..6808)
                     /gene="taxA"
                     /locus_tag="JSU10_RS24870"
                     /old_locus_tag="JSU10_24865"
     CDS             complement(6305..6808)
                     /gene="taxA"
                     /locus_tag="JSU10_RS24870"
                     /old_locus_tag="JSU10_24865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000781813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxosome-coupling protein TaxA"
                     /protein_id="WP_000781813.1"
                     /translation="MKMIAFRLSDDELKFAEHNAILSGFTSINAFAKHNVLNIETKPVN
                     IPVNNEPAKLVSVRLYPHEIELVKRNAALHGMSMSREIAIRVRQSLLKSEVCLYPDEVK
                     ELKKLSTAVDRVGRNIHFIIKGERFCTVNDPDFRKDVIEVIELCKQIDSKLETLTKSVV
                     NRFG"
                     /name="taxA"
     gene            complement(7169..7516)
                     /locus_tag="JSU10_RS24875"
                     /old_locus_tag="JSU10_24870"
     CDS             complement(7169..7516)
                     /locus_tag="JSU10_RS24875"
                     /old_locus_tag="JSU10_24870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000681609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="WP_000681613.1"
                     /translation="MKHLKYKGYLGTVEPDFENNVLYGKLAFIRDLVTYEASTLAELEQ
                     EFKTSVDLYLQSCVEDGKEPDTPFKGVFNVRLDPELHRRVAEMAMEEDLSLNAFVNKAL
                     EKEVSNHRAGA"
                     /name="type II toxin-antitoxin system HicB family
                     antitoxin"
     gene            complement(7513..7761)
                     /locus_tag="JSU10_RS24880"
                     /old_locus_tag="JSU10_24875"
     CDS             complement(7513..7761)
                     /locus_tag="JSU10_RS24880"
                     /old_locus_tag="JSU10_24875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005653200.1"
                     /GO_function="GO:0003729 - mRNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family toxin"
                     /protein_id="WP_000520549.1"
                     /translation="MGKTDKLLAKFLNSKKTFEWDELVVLFSSLGYVKKEMQGSRVRFF
                     NAEINHTILMHRPHPESYIKGGTLKAIKQNLKEAGLL"
                     /name="type II toxin-antitoxin system HicA family toxin"
     gene            complement(8066..8416)
                     /locus_tag="JSU10_RS24885"
                     /old_locus_tag="JSU10_24880"
     CDS             complement(8066..8416)
                     /locus_tag="JSU10_RS24885"
                     /old_locus_tag="JSU10_24880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000854261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000854259.1"
                     /translation="MKTLKQAAMQFASDLRNNRCFKAAYNDALLELDREDLNAIVQCTF
                     LFEPKRTLAELDKLIKKIEVEEDYEFCVSLDKGKGLHEVRTFKSEREALDAYRILTLYG
                     YTVTIEKKEGND"
                     /name="hypothetical protein"
     gene            complement(8507..8806)
                     /locus_tag="JSU10_RS24890"
                     /old_locus_tag="JSU10_24885"
     CDS             complement(8507..8806)
                     /locus_tag="JSU10_RS24890"
                     /old_locus_tag="JSU10_24885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000673428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000650309.1"
                     /translation="MKCSSVFTSTTNHVFTFERVTLCTIILMHKDTGQQYVVIFTDNNK
                     IRDYKAGIVPQFGELKQSDVDLVLFYRDEYEKYFDSLKDGDECLSFKDFIECLC"
                     /name="hypothetical protein"
     gene            complement(9260..9487)
                     /locus_tag="JSU10_RS24895"
                     /old_locus_tag="JSU10_24890"
     CDS             complement(9260..9487)
                     /locus_tag="JSU10_RS24895"
                     /old_locus_tag="JSU10_24890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000801440.1"
                     /translation="MKPDNTPENVKKLRLKAGLTQKECSHIYGVGLRTWQKKEEVNTQN
                     SQSLSLVEFEFLLLLAGEHPEYVLCKRESK"
                     /name="hypothetical protein"
     gene            complement(9474..9725)
                     /locus_tag="JSU10_RS24900"
                     /old_locus_tag="JSU10_24895"
     CDS             complement(9474..9725)
                     /locus_tag="JSU10_RS24900"
                     /old_locus_tag="JSU10_24895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001565860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001230707.1"
                     /translation="MRKNKGDVTYFLEKEGDNYRLTKRIKARTNVKIGNKTTKITLYDA
                     VLNENELQHIDFTCAGLREDDETPVKNLIKEFMLNETR"
                     /name="hypothetical protein"
     gene            complement(9722..10264)
                     /locus_tag="JSU10_RS24905"
                     /old_locus_tag="JSU10_24900"
     CDS             complement(9722..10264)
                     /locus_tag="JSU10_RS24905"
                     /old_locus_tag="JSU10_24900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001401654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001083906.1"
                     /translation="MNSFFEQYHPVFEVVCRILGNGWRVNKLDDCSSRIKLTSPQFKNY
                     SVHIRMEKDRFSVVGSVDSRSWRSPHHVCTLSRKRNPVDIAADIERKILVNASQEVLQA
                     IEYEKHQVEKKDEILILKGMLSQLVQLESWYGALTGFKAENGLNGKVTEQGDSYDLQIR
                     GLSIDQLVKITGYLKQL"
                     /name="hypothetical protein"
     gene            complement(10369..10884)
                     /locus_tag="JSU10_RS24910"
                     /old_locus_tag="JSU10_24905"
     CDS             complement(10369..10884)
                     /locus_tag="JSU10_RS24910"
                     /old_locus_tag="JSU10_24905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025391.1"
                     /GO_process="GO:0006457 - protein folding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="WP_001025395.1"
                     /translation="MNIQEALNVFGLSGELTEKDIKAAYRKAALKYHPDRNPLGAELMK
                     AVNAAFDVLMANIDKINQFQSTDEHARYNYGDDLEKVLNVLSGLSGLVFEVIGNWVWIS
                     GETITHKETLKEIGCKWAAKKKQWFYRPDEHKSYWNREEHTIEEIRAKYGTTGQRRATG
                     WQRVETRA"
                     /name="J domain-containing protein"
     gene            12294..13157
                     /locus_tag="JSU10_RS24915"
                     /old_locus_tag="JSU10_24910"
     CDS             12294..13157
                     /locus_tag="JSU10_RS24915"
                     /old_locus_tag="JSU10_24910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001473366.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_208235563.1"
                     /translation="MTTNKTSLSRLTKVRHRNELNSTLSTLPMAAKKVLFLAMCQINSK
                     NEFDDDHIFYVTVADYIKWVQVKPDAAYLALRDGSNILDTTLLKLKHDEILELSSDLGF
                     KFTKSNVPDSMNLSLTVFSTYYRNEGRVGIKFTKEAKRFLCKLIGEEKRYTTQVLLSVV
                     RLTSVNAASLYQLIRKIYSNNSRANSFEMTIDELKDELNLYTIGAGVKDYKYPDYPAFK
                     RDVLNKSVKEIMKHTEVKNLSFVVSEKIGRKVYKLKFSYTIGYEGDTREDSEFTNMFDK
                     MYPPEN"
                     /name="replication initiation protein"
     gene            13170..13574
                     /locus_tag="JSU10_RS24920"
                     /old_locus_tag="JSU10_24915"
     CDS             13170..13574
                     /locus_tag="JSU10_RS24920"
                     /old_locus_tag="JSU10_24915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012421203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_012421203.1"
                     /translation="MKDNIVDFQRIRDSMNDHSTDVANDAVYSDKKKISRFFYLLTPVR
                     YLLAASLYLVVVIPLGILAGFRFLLQFLGGIAVMGLTIAWYMGADVSSYVVLAAWIALT
                     VCGCAEQIFEWWIESRFAFRLFGIGRTPEE"
                     /name="hypothetical protein"
     gene            13709..14236
                     /locus_tag="JSU10_RS24925"
                     /old_locus_tag="JSU10_24920"
     CDS             13709..14236
                     /locus_tag="JSU10_RS24925"
                     /old_locus_tag="JSU10_24920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001473365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2726 domain-containing protein"
                     /protein_id="WP_000577043.1"
                     /translation="MIFNRNKKRDEQHNSAVVLPSAENVFSDFIKNNPALTTNAFTAIG
                     EVDCFTKVPLLSNNESEFLGILSRNIIPDYSVFPKIRLIEFVRPHGSEEAVKDLIREVQ
                     NITCDFVLKSYDETVLAVIQFGETATVRQQKKKLIIQRVCAMTGISFFEFKNTVDMMGS
                     EDFCQFLRDDEE"
                     /name="DUF2726 domain-containing protein"
     gene            complement(14627..15349)
                     /locus_tag="JSU10_RS24930"
                     /old_locus_tag="JSU10_24925"
     CDS             complement(14627..15349)
                     /locus_tag="JSU10_RS24930"
                     /old_locus_tag="JSU10_24925"
                     /inference="COORDINATES: protein motif:HMM:NF028536.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAP2 family protein"
                     /protein_id="WP_039003037.1"
                     /translation="MPYLSNKRLLAEMSIALVMAIVATLTLEHSQIDLMVADWFYLGMG
                     HWMVAKQAFLPDLLLYSGLKKLLMAMLIYLLVATICRAYHEKKGNAITAKWLVPVTKFR
                     VRELAYLVLTLILVPTVVASLKAYTHVVCPVHLTIFDGTLPYLPMLDSMRNTIPDKCFP
                     AAHASSGFALFAFAFAPSLRRRRGAIIIVVMALGWAMGCYKMIIGDHFLSHTVVSMMLA
                     WAMSAGLAWVFFKKGEQV"
                     /name="PAP2 family protein"
     gene            complement(15421..17046)
                     /locus_tag="JSU10_RS24935"
                     /old_locus_tag="JSU10_24930"
     CDS             complement(15421..17046)
                     /locus_tag="JSU10_RS24935"
                     /old_locus_tag="JSU10_24930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_049589868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine--lipid A transferase MCR-1.1"
                     /protein_id="WP_049589868.1"
                     /translation="MMQHTSVWYRRSVSPFVLVASVAVFLTATANLTFFDKISQTYPIA
                     DNLGFVLTIAVVLFGAMLLITTLLSSYRYVLKPVLILLLIMGAVTSYFTDTYGTVYDTT
                     MLQNALQTDQAETKDLLNAAFIMRIIGLGVLPSLLVAFVKVDYPTWGKGLMRRLGLIVA
                     SLALILLPVVAFSSHYASFFRVHKPLRSYVNPIMPIYSVGKLASIEYKKASAPKDTIYH
                     AKDAVQATKPDMRKPRLVVFVVGETARADHVSFNGYERDTFPQLAKIDGVTNFSNVTSC
                     GTSTAYSVPCMFSYLGADEYDVDTAKYQENVLDTLDRLGVSILWRDNNSDSKGVMDKLP
                     KAQFADYKSATNNAICNTNPYNECRDVGMLVGLDDFVAANNGKDMLIMLHQMGNHGPAY
                     FKRYDEKFAKFTPVCEGNELAKCEHQSLINAYDNALLATDDFIAQSIQWLQTHSNAYDV
                     SMLYVSDHGESLGENGVYLHGMPNAFAPKEQRSVPAFFWTDKQTGITPMATDTVLTHDA
                     ITPTLLKLFDVTADKVKDRTAFIR"
                     /name="phosphoethanolamine--lipid A transferase MCR-1.1"
     gene            complement(17140..17844)
                     /locus_tag="JSU10_RS24940"
                     /old_locus_tag="JSU10_24935"
     CDS             complement(17140..17844)
                     /locus_tag="JSU10_RS24940"
                     /old_locus_tag="JSU10_24935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000052343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2806 domain-containing protein"
                     /protein_id="WP_225236207.1"
                     /translation="MSLIDLSLSGLSEPGTKLIEKISDAIGVLYEPTRIRKKAKAEAEA
                     KRTELISRLELEGIEKRAVERFLKRETKRQENIENITMQAAQSLSESDNVSDIDEDWIE
                     AFFRECEDISDEQMQMLWGRILSEEAKSKGSFSRRTLKLLSTISKEEANLITYFGKFVW
                     QANKLTPILFTDENGDTEGITFDKLSVLDSLGVIQQGIGYSLQIINTLKCIFSMGLRND
                     CLIKFLKYFCIA"
                     /name="DUF2806 domain-containing protein"
     gene            complement(18005..18274)
                     /locus_tag="JSU10_RS24945"
                     /old_locus_tag="JSU10_24940"
     CDS             complement(18005..18274)
                     /locus_tag="JSU10_RS24945"
                     /old_locus_tag="JSU10_24940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001086090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001086090.1"
                     /translation="MAKLELPELEQNQEVNATKFVQDAGKRPTENKTKLASFRIPNELL
                     EFIDNESKRLHRPKTKIIKAALLAYQDLDENELINLWFRADRND"
                     /name="hypothetical protein"
     gene            complement(18349..18996)
                     /locus_tag="JSU10_RS24950"
                     /old_locus_tag="JSU10_24945"
     CDS             complement(18349..18996)
                     /locus_tag="JSU10_RS24950"
                     /old_locus_tag="JSU10_24945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001694199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division plane positioning ATPase MipZ"
                     /protein_id="WP_000587671.1"
                     /translation="MIIVIGGDKGGTGKSHLATNLTVCLSQQGKRTGLVETDLNGSTKK
                     WNKRREQAGLPPVALNEAYGDISAKITKMADVAEILIIDTAGYDSTEFRTALKVADIVI
                     VPIDPLAQVEADSLQTVTKIVRDAQKINPRLAAHVLLYKCQPNTFSEQQELRDSLNSHD
                     YWLKPMKSTVSFLRAFVRAMNQGMGVHELKSNVSGASQAKAQIELLLKELEL"
                     /name="division plane positioning ATPase MipZ"
     gene            complement(19659..19754)
                     /gene="dqlB"
                     /locus_tag="JSU10_RS25645"
     CDS             complement(19659..19754)
                     /gene="dqlB"
                     /locus_tag="JSU10_RS25645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinQ-like type I toxin DqlB"
                     /protein_id="WP_021534057.1"
                     /translation="MINMLIIVLRAVVAVANALIAVLELIRQFID"
                     /name="dqlB"
     gene            complement(20533..21237)
                     /locus_tag="JSU10_RS24955"
                     /old_locus_tag="JSU10_24950"
     CDS             complement(20533..21237)
                     /locus_tag="JSU10_RS24955"
                     /old_locus_tag="JSU10_24950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221000.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS26 family transposase"
                     /protein_id="WP_001067855.1"
                     /translation="MNPFKGRHFQRDIILWAVRWYCKYGISYRELQEMLAERGVNVDHS
                     TIYRWVQRYAPEMEKRLRWYWRNPSDLCPWHMDETYVKVNGRWAYLYRAVDSRGRTVDF
                     YLSSRRNSKAAYRFLGKILNNVKKWQIPRFINTDKAPAYGRALALLKREGRCPSDVEHR
                     QIKYRNNVIECDHGKLKRIIGATLGFKSMKTAYATIKGIEVMRALRKGQASAFYYGDPL
                     GEMRLVSRVFEM"
                     /name="IS6-like element IS26 family transposase"
     gene            complement(21324..21788)
                     /locus_tag="JSU10_RS24960"
                     /old_locus_tag="JSU10_24955"
     CDS             complement(21324..21788)
                     /locus_tag="JSU10_RS24960"
                     /old_locus_tag="JSU10_24955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001293133.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="H-NS family nucleoid-associated regulatory
                     protein"
                     /protein_id="WP_001293134.1"
                     /translation="MSELTKEDEYGIISRTMMNIRSLRVFAREIDFEQLLEMQEKLNVV
                     IEERREDAEREAAERQELEAKRQQAIEYIISLGLDPESLLAPVTADTVKTRRKAKGGVR
                     KAKYRFKDENGEIREWSGNGKRPLALQKLLDEGHFMEDFLIEKTKPEQAE"
                     /name="H-NS family nucleoid-associated regulatory protein"
     gene            complement(21806..22012)
                     /gene="hha"
                     /locus_tag="JSU10_RS24965"
                     /old_locus_tag="JSU10_24960"
     CDS             complement(21806..22012)
                     /gene="hha"
                     /locus_tag="JSU10_RS24965"
                     /old_locus_tag="JSU10_24960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulator Hha"
                     /protein_id="WP_000850859.1"
                     /translation="MKTKQEWLFQLRKCTSRDTLEKVIEINRYKLPLSESEAFYSAADH
                     RRAELVMNKLYDKVPSGVWKYVH"
                     /name="hha"
     gene            complement(22009..24159)
                     /locus_tag="JSU10_RS24970"
                     /old_locus_tag="JSU10_24965"
                     /pseudo=""
     CDS             complement(22009..24159)
                     /locus_tag="JSU10_RS24970"
                     /old_locus_tag="JSU10_24965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247728.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0003916 - DNA topoisomerase activity [Evidence IEA]"
                     /GO_process="GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase 3"
                     /name="DNA topoisomerase 3"
     gene            complement(24164..24736)
                     /locus_tag="JSU10_RS24975"
                     /old_locus_tag="JSU10_24970"
     CDS             complement(24164..24736)
                     /locus_tag="JSU10_RS24975"
                     /old_locus_tag="JSU10_24970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012908955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001198960.1"
                     /translation="MQYALFDGFERKFLLDALEFGVLKDWKENPVKELPDIDESAHPFH
                     ICYGGYLLNPGVSDSDISRKIKDQAGFWLAAIDDTRMDCHSIAYYDIHTLPLISCGHQK
                     IVPFAALIKADECIISKIASYSGFAVTAFLRIKEWDIATNILNREGIFAFNGCERRFRV
                     VSKDNWQHTVSEERAIRCAKRLIQCKG"
                     /name="hypothetical protein"
     gene            complement(24740..>24934)
                     /locus_tag="JSU10_RS24980"
                     /old_locus_tag="JSU10_24975"
                     /pseudo=""
     CDS             complement(24740..>24934)
                     /locus_tag="JSU10_RS24980"
                     /old_locus_tag="JSU10_24975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000517490.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /name="ATP-binding protein"
     gene            complement(25047..25352)
                     /locus_tag="JSU10_RS24985"
                     /old_locus_tag="JSU10_24980"
     CDS             complement(25047..25352)
                     /locus_tag="JSU10_RS24985"
                     /old_locus_tag="JSU10_24980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001350475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrbM/KikA/MpfK family conjugal transfer protein"
                     /protein_id="WP_001350475.1"
                     /translation="MTGKNRVVLPALLALGLISTNANASDPCASVLCLYGKAVGQGGGS
                     ECRSAEKDFFNILKKKKGSIRWSKTFDARKAFLNQCSTADPAAISKIMSKFGRVRG"
                     /name="TrbM/KikA/MpfK family conjugal transfer protein"
     gene            complement(25336..25758)
                     /locus_tag="JSU10_RS24990"
                     /old_locus_tag="JSU10_24985"
     CDS             complement(25336..25758)
                     /locus_tag="JSU10_RS24990"
                     /old_locus_tag="JSU10_24985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001353737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cag pathogenicity island Cag12 family protein"
                     /protein_id="WP_015059903.1"
                     /translation="MMKKLVFLLISILAGCSSPPEPTPVQFEKANEVINPSLPYVPDFH
                     GVIKSDVSGKGWVYEITSLSGVQARTPTFYYALAHADRIVVTTHDAGLWFRIRDLLKME
                     GATAVVEWRNEKSFLPEQVKIVFIKAQNEVKNDWKK"
                     /name="cag pathogenicity island Cag12 family protein"
     gene            complement(25767..27629)
                     /gene="taxB"
                     /locus_tag="JSU10_RS24995"
                     /old_locus_tag="JSU10_24990"
     CDS             complement(25767..27629)
                     /gene="taxB"
                     /locus_tag="JSU10_RS24995"
                     /old_locus_tag="JSU10_24990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000847106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxosome-coupling protein TaxB"
                     /protein_id="WP_170324155.1"
                     /translation="MKTFNKSQSVFIFAVMLVAAWFAGSFIFFVLYFSQIKNLKPLKAV
                     FRSIDAYRMDIFTDSLTLSVVTTDIRVLAFVALGAGFVVSLIIPVAALIKLNEKKENIF
                     GDARFATINDIRESNSFTLDGDEKDGIIVGIKDKKIIRYVGAAFSAMGAGTRAGKGAGI
                     VITNLMKYWWSVIILDPKRECFNITSLIRKVILGHEVYKFDPFSSVTHRFNPLYYVSMG
                     TSEGFNQLENLSLIIYPYKTDGADAGSYLNKTAGGVFKSYAVALWFMIKNDKAGLKTLD
                     IEPVFSMSKINQLFERAEPEHLLSFMNDIRSELRGKDKTLADIGVAGLKAFIEMEDKTK
                     AQLKSNFLNGLSPFANPNVANATDGNDFDLRQVRKKRMTIYFCISGDNARLAEKITNIF
                     FQLAIQVNLEKMPTDDPEIKHDCLFLLDEFPSIGAVDYIKSKSGLIAGYKLKLLIVYQV
                     GSQLEEIYSYAGSKTLLASAPCKIVYSASDVKDARELSEAMGTRTVTIGSKSKSRSRGG
                     TSRSESESLIERPLVTTNELLTLKFSEEILAMKGENPIRCEKAFYYLNEYFFGDFVKVA
                     PELANIFPRKKGKLVMPPQKVFENEIVAKGYLVVKDVPDLDKAA"
                     /name="taxB"
     gene            complement(27616..28644)
                     /gene="virB11"
                     /locus_tag="JSU10_RS25000"
                     /old_locus_tag="JSU10_24995"
     CDS             complement(27616..28644)
                     /gene="virB11"
                     /locus_tag="JSU10_RS25000"
                     /old_locus_tag="JSU10_24995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001401678.1"
                     /GO_component="GO:0043684 - type IV secretion system
                     complex [Evidence IEA]"
                     /GO_process="GO:0044097 - secretion by the type IV
                     secretion system [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-type DNA transfer ATPase VirB11"
                     /protein_id="WP_077249170.1"
                     /translation="MSGVIPVSISNKSLDFYKNQVFQSFLDIEGLTEIAVNRPGEIWTE
                     INGDWTFHNNDSVTFDFCKRFSHTLASFRGDEIGETKPLLSATLESGERVQVVFPPACE
                     RNTISITIRKPSTRQITHKEYVANGFYDYVQSGKKHRTYDDELLDLFRARNIAAFMELA
                     VAAGKTIVFAGATGSGKTTYMKSLIDFIPLNTRLITIEDAEEIKFFIHKNYVHLFYPSE
                     SGSDSGSIITAAKLIKSCLRMKPDRILLAEIKGGDAWDFVKVTGSGHSGSMTSIHAASA
                     KDAIIQMVTKCYQNRECQNLPFDVLRKIIMDSIDIVVHVGRDGVVRHMSDIYYKGAECE
                     NF"
                     /name="virB11"
     gene            complement(28646..29764)
                     /gene="virB10"
                     /locus_tag="JSU10_RS25005"
                     /old_locus_tag="JSU10_25000"
     CDS             complement(28646..29764)
                     /gene="virB10"
                     /locus_tag="JSU10_RS25005"
                     /old_locus_tag="JSU10_25000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirB10/TraB/TrbI family type IV secretion system
                     protein"
                     /protein_id="WP_000131271.1"
                     /translation="MTDMKKTDDLVDDANQSKLESKPVHDIGDIRNKNNRMRSGFLFGL
                     MAVLVIGIFALKTYKNYFADDQQKASESTGDTSISQVSKIRTGLGQNFDPVENKAIINP
                     GATGSGNTVSDGHKEVPQEGFRKYLSIPVAGQGGSQTGASGSSRTSQTSEPQEERKESK
                     ENVPGKSGMKVTAINLDPDLYIEENRLIPCALTTRFVSDVAGRISCVFTEDVWSANHHT
                     KLLEQGTKAFGRYQTGTLNHGQGRMFVMWEQLRTPDNKRIDMVNTAAAGPLGEAGIDGW
                     IDSHFWERFGGSLMLSMVQDVAAAAADNAPGKDRNVDYTENSRQAMAEMAKVALENSIN
                     IPPTMYKNQGDIITIMVGEDIDFSDIYELKVK"
                     /name="virB10"
     gene            complement(29757..30665)
                     /gene="virB9"
                     /locus_tag="JSU10_RS25010"
                     /old_locus_tag="JSU10_25005"
     CDS             complement(29757..30665)
                     /gene="virB9"
                     /locus_tag="JSU10_RS25010"
                     /old_locus_tag="JSU10_25005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001299943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-type conjugative transfer protein VirB9"
                     /protein_id="WP_001329500.1"
                     /translation="MMKKLFIATCLLLPGLVYSAATPLPSGFDARMQTVSYNGANTTVI
                     RSKTGFLTSVVFDEGEAVISAKAGFPAGWEITTDDNVVYINPRPVVQEQEGDEGEKLKK
                     VFQPTEKEWDTNLFVRTTKRIYSLDLILLSEEKQAQPAYVVQFRYPSEIAKKNAEEVRL
                     AKEKQEKLRQKKLISESFEKADAPKNWNYFMRVNEKYDSRRIAPDFAYDNGIFTFLGFN
                     SGKVFPAPFAVRDGQEQTLAFNVETKGKYKIMVIHNVNDKFVLRYGNSVVGVVNKSFGK
                     VLTDQRNTSSPAVERVEVNND"
                     /name="virB9"
     gene            complement(30662..31348)
                     /locus_tag="JSU10_RS25015"
                     /old_locus_tag="JSU10_25010"
     CDS             complement(30662..31348)
                     /locus_tag="JSU10_RS25015"
                     /old_locus_tag="JSU10_25010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001290765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein"
                     /protein_id="WP_001290765.1"
                     /translation="MSEKIEKKIETAKSFERQLYAENERSKKNAWRVAVAASLLSLCLG
                     AAICVLVPLKEKELAIVAVGEKTGRTELITQVKQEKILQSEALGRYFVNTYITLREGYN
                     YPSLQYDYETVQLYSSNTVKDDYLRLYNSDMAPDKIYHNNGSYVAVEVISNIISDATAP
                     DKLASVRFKKTTRNFTTGQVSVSYWTARVTYRFEPEKSVKSSSRELNPLGFTVTSYQTD
                     REVRGE"
                     /name="type IV secretion system protein"
     gene            complement(31863..32957)
                     /locus_tag="JSU10_RS25020"
                     /old_locus_tag="JSU10_25015"
     CDS             complement(31863..32957)
                     /locus_tag="JSU10_RS25020"
                     /old_locus_tag="JSU10_25015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001677777.1"
                     /GO_process="GO:0030255 - protein secretion by the type IV
                     secretion system [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein"
                     /protein_id="WP_000046889.1"
                     /translation="MAQGFFVKYNSTVMDSVDKISSSYQTQFANDIMSLATVSVTLYVL
                     WKGYQILASKTQTPLQDLVWDLSKFAIIIMFITNADGYLTAATDALQGMKDGFSGGVSV
                     WQTLDNLWKSTQNLGAEIYSLDKSTYVKDQGVVGQFLIWTGSLILMAVSVVVFLTADVT
                     MKLLIITAPIFIFCLMFGFIRSMFNNWLQSLFSSILTVLFASLVIRIAMDFQGMILSHA
                     IRAAQTGNVNLVSTGAMGFMAGFLGALLVLIAKGFAVQLAGAGVEGAVQGAAMMGLGAA
                     GMATGKSLMLGGRAGLGFGLGMAGRTGMNSLSGKAGNLMGRGARTAAEWAGEKGYQALA
                     PTGALGMKARRLASLEKARARNAA"
                     /name="type IV secretion system protein"
     ARG             complement(15425..17046)
                     /name="mcr-1.1"
                     /gene="mcr-1.1"
                     /gene_name="phosphoethanolamine--lipid A transferase
                     MCR-1.1"
                     /tool="amrfinderplus"
                     /drug_class="COLISTIN"
                     /identity="100.0"
                     /coverage="100.0"
     Typing          12289..13157
                     /name="IncX4"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="99.656"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(5076..6301)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.511"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(25771..27629)
                     /name="MPF_T"
                     /typing="mate-pair-formation"
                     /tool="MOB-suite"
                     /identity="98.71"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(29761..30665)
                     /name="MPF_T"
                     /typing="mate-pair-formation"
                     /tool="MOB-suite"
                     /identity="99.669"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(30666..31348)
                     /name="MPF_T"
                     /typing="mate-pair-formation"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="1.4399999999999998e-137"
     Typing          complement(31891..32957)
                     /name="MPF_T"
                     /typing="mate-pair-formation"
                     /tool="MOB-suite"
                     /identity="99.719"
                     /coverage="99.0"
                     /evalue="2.69e-172"
ORIGIN
        1 gttcatagcc cgtattacac atatcgaagg ctccatactt gctgaacaga tttttagcct
       61 ggctgctcaa tgcaccttcg ggattactgg taaaatcaga aatataactg ttcccctgct
      121 tataaatatt tgtcagttct gattgtagat tacttagttc atttgtgaat gccgcaatat
      181 tccttatacc tgttgccgtg gctaactggt ctttatatgc ctgaatctga ctctgataat
      241 gttgcgcagt ttcggaccat tgtttgagtc gttctgtcca tcttgcggct tcaacagccc
      301 attccggact ggcatcaata gcaaccggaa ttcctgcgct actataaaat gaagccgtca
      361 ataatgatat cagcatcaat gtttttacac gtttcataaa ttcacctgtc agattgcccg
      421 ttcgagatat tcaggaaccc agtcatcagg ctccattcct tcatgataaa tactttcaaa
      481 aatttccaga ttatctgcac tggcagacaa tattttcaga tatccaccaa gtcccgataa
      541 atcgagtgtc gcaagtgctg aaaatgattt accatccccc tttttaagac tgcttttttt
      601 aatcaggaac tgtctcgaca ttggatcaag atttttaaca atattaaatt cttctggtgt
      661 cagcttaagc ccttccacat aatcattgta atcggcatca gggttagcca gataaatact
      721 ggtgctgcat acttcaacca cagcccgtga aatcttattc ttcagtattt catccggaga
      781 ctgagttgcc ggaataacca gcccgttcag cttacgaatt gttttcagct tgttatagac
      841 aaagtcgctg aatgcttcat cctgtagcca tttccagaac tcatccagga agataacaag
      901 acgccgaccg tccagaagct gcgtaatgcg atacagcaga tagaaagtaa ttggtgcaca
      961 taccatcgga tcatcaagaa actcggtgcc atcaatgcca aaattattga catgctgaat
     1021 attgaatgtg tctttagcat tatcaaaaac ccagccatga gcctgaccat tagcccattg
     1081 ggacaaacga aggataatcc cgttctcctg ctcatcccga tcatctctct gcataagatg
     1141 ctcaagcatt ctggtaatgc catattcccg catttcgcca ggaggaaaat ccatcacagc
     1201 atcaacactt tcgctaataa gcaatctttc tcttgtcgat aatctttcac catttctggt
     1261 acacagaatc tccatcagtt gcttgacaaa aatacggtta cgttttgttg cctccagagc
     1321 aaaaggattc catcctgtcg gctcaccgct ttttacggtg taatattccc caccaagcat
     1381 tcggatacat aattcagccc ctctgtcctt atcaagaaag acacacgtta acttcttatt
     1441 ttttgcagaa tcagcaaaag tttcggggtt attgtatttc tgtaatgaac aggcaagata
     1501 tgacagaaac attgtcttac ctgaacccgc cgttcctata actttagtat tagcaagatt
     1561 cttttcacct gtttcatctt taaacagtac tgagttatgt aaattcagat aatacgcctg
     1621 tttgcttggt gttttgagaa tagcaaccgc ttctgtccag cagtttttat cgcgtttccc
     1681 ctggtagaaa ttatggaatg atgccagttc aacaaaattt acattcgata ccggagataa
     1741 acgcggcctt aaatgaaaaa ccgccggaag ttgggcaaaa tatgccgctg gcaatgacat
     1801 ggttgacaat gccggaataa tgcccaggtc agtaaagtct gccgtaattt tacttacaga
     1861 ttcgtcaagc tccctgatac tgtccgccat taccattagc gtgaaatggt agtaaccaaa
     1921 ggatatatca ccggacacaa tatcattttt tgccttttcc agttcctgca attgagtaac
     1981 cgcatcatct tctgtactct ttaactgttt ttccgcacgt ttaatggtac tcaatgcttc
     2041 tgatttgctc atggatgtat atgaatgcgt aataatatag tcagcatcag aatactggag
     2101 cacatcaaaa acaccggatg cagtctcact acagaaatct ttaatttcaa tagaacgaaa
     2161 gaatttattt ccatcaatgc ggcatatctg tccggtatca tgattaaaaa atacatccac
     2221 tccaccaaga acgttataca ccggagaatc agtgactctg attttttgca acttccctgt
     2281 cagaagataa ttatagaatg acagttgaga agaaaatacc tttccgttct cctcatacat
     2341 accaagaagt ttacaggtaa atttatcaag agcgccagag aatgtattaa tcatttcatt
     2401 cattctcttc acatgaatgt taatatcatc tttcttttct ttaatacttt ttttgcgttt
     2461 ttccagtttt tctacacgcc catctggtct gtagatcagc gtaaagtaaa gagtgttccc
     2521 cttgaattta tttttcttca tcgaaccata gtaacaatct gatattatat cagcatattt
     2581 attacctgat tttgtggtaa aggcatcata aaaactcgcc ctgcatgaat ggttataaaa
     2641 tgaaacgggc agtccggaaa atgaacgaac aagagtggca acctgtgagt caatagtttc
     2701 taacatctcc gcagagattg actcaaagtt aatccccatc agttgccatg tcgctacata
     2761 atcaccgttc tttgttttga caatattttt atcaacatgg cttgaatagg gtatatattt
     2821 accagtagtg attcgctcat tcagtttcat ggcattaaca aactcatcaa tttcaacagc
     2881 ttcatattgt ccggagagga acgctcgtgc gccaaaaaaa cgattacaca ctgagtttcc
     2941 gagcattttc agcttaagaa agtaaaggtt aaaaatgtgg tcgtcctgtt tagtgatcat
     3001 tctgagaaca aaaaccgcga caggaatagc cagccagatc agcttgctga tataaacccc
     3061 cgccagaaat aacgcccctg caaccacagt aagagggacg acaggaacac ccacatacat
     3121 agcagggcgt gtcatcgctt tataaagtgt agacatacta tcgtccccgc tttattagtt
     3181 aaccatcatt gatgcaactt cagccgcact ggcgataaca attgccccga tgatgatagg
     3241 tgagcattca cgaatcgtgc ttccaccaaa cagaaccttg tagccgaccc acagacaggc
     3301 aatagtcacc gtgaccagtg aaaggccact aagccctgtt ttaacctttt ccagcagtgt
     3361 ttccgctttt gaaaatccat ccgcaaaggc cggtgatgca actgttgccc cggcgactgc
     3421 cataactacc ttatttttac gctgcattac agcagtgaca gatgaactga tacgattaca
     3481 tactgatttc ttaaatgaca ttttataagt cctgttaatt aataactttt gttggatgtg
     3541 agttatcaac aaaagcagat ttcagaatgg tttcgggcca gataactgac gcattgtgtg
     3601 aacgattttt tcgctgttct cccccgttgc ttcttgtacc tggtacaaca tatttttttc
     3661 gttccctgta taaccaattc gctcaacata acttgtatta ttaaattctt tttcttttcg
     3721 cttaccgtta ctaaaatttc ctgtatagta acaactaagc gcattctcca ggctcctccc
     3781 tcgtttataa caatccacga taatcttttc gaaaacagac aagttttcac acgaatcaag
     3841 gagtttttct gcggacgtgg aataaaaaga aaaattactg ctggtaatct gcatcaatcc
     3901 gacagaataa cgtctttttc tgttttcaat ttctctgata actgttagag cttcctcctt
     3961 acttttgggc atatgactga taacactttt gccatagcta ccgcgttccc tttcaggaat
     4021 aatttcagcc acagcataag gattgaagcc actctcgacc cgtgcaatat catttatcgt
     4081 atccggatga actgacgccg cacacagcaa caaggctggg aataaagacg ccatagctaa
     4141 cgctccatat atccatcaat acgcatactc tgcattaata agtaaataat ttgcttcctc
     4201 ttactaagca aagatgtttt cgttttctct tcgataaatg cattcaacct gggatcgagt
     4261 tttatcgtga cataaacccc tttttccgat gacttatctg atttctcctt catttaatca
     4321 atccactata agtgtgtttt ggatttgttt tagatgcgaa catgattcgc aaaataacaa
     4381 tgctcaacaa tacgatcaaa taacattgac aaaatggtca tattatgatt cgtgtgatca
     4441 tgcaaaaaaa agcgcccata tgggcgctct agtatgattg aaacattagt taatcgaatg
     4501 tagggactgg agcatccttc tgagactgaa taaacaatgc ttgtttaaca ttcttacagt
     4561 tctgagaatc agagccagac ttctcacact ctgcctgctt ttgtttagct tccgtaagat
     4621 gctgccccca ccaatccaca ctttttactt cttcactaca accaacaacg aagaacggca
     4681 aagcaaaaag cagatatttt ttcataaatt ccccctctca ctgatcctgg aaagacttga
     4741 cacgagaagg gcattagtcc gaatgagtac cagtgttctg atccctgcca acgagacacc
     4801 tcccagttca gctcttacag aatcgagata ttttctgaca gttgaaggct ttagtccttt
     4861 gaaatcagca atttcattca gggtatagcc ataagcgtac atcagaataa tgtctttttg
     4921 ttgctcactc aaatgttctt catcactaaa aatttgatga agtccagaaa tgcagatagc
     4981 atcatgctca gtcatgataa cctccctcgt aggttgttgt gatcagcagg tgagcaaagt
     5041 agccgctttg ttcacctgcg ccaaaatcaa attaaaatcc caggcctttc cagttcctct
     5101 cttgctgtat tcttagtttt ttatgttctt ctgaatcctt tgtaaacccg tgttcaagag
     5161 caaacccgat attgtgacct tgctcatgtt ttctggatag ctgcttttta acgtcgctct
     5221 cttccggctg cctggtttca cgctcatgaa cctgacgttc tctgttccgc tcaagggcaa
     5281 tattttcaat ctgccagtta ttgcgatgcg tttccctgta gcctgcaaca gttccatcat
     5341 cattaaacat tggactacgt acaagcgtag gagccagttt ttttactttg ataagcgttc
     5401 ctggctggag cttttcagac tcgaaatgat ttttcaggtc tttaccccaa atcgtaacct
     5461 gttttccacc ttttaaagtt tcataagtga ggaaaggtgt tcgtttttcc cctgccttat
     5521 tctgatatga agtctcccca aaatccacca ccttgtaaac attccggagt cttttcggat
     5581 tttcagcctg taatcgctca atctcccgtt tacgttcggg atcttttttc caggtagcgg
     5641 taacatcata tcccatacct ttcagttttc tatgaaaacc gtttctgaac ttcctggttt
     5701 cagatttagt aagtcttaat cttcttcctg tttcttcatc acgaagttta atattaacat
     5761 gtacatgagg atgatcttct ttatcatcat ggtatacggc aacgaaagca tggtttggat
     5821 aagtctcttt aagaaattct accgtagctt tcagagcgtc ttccctgctt actccagctt
     5881 ctggtggtgg tgaaaagacc atgtttttca catgatcgat gttttctcca cgatatgtcc
     5941 tggagtaatc atttccttta gataaagttg aagtaaactc cctattaaag tcttctcctt
     6001 tccctgtttg ctctgaacct tttccatcat aacagtaagc ctcaagttca ccattacggg
     6061 taatgtaatc aataccattt ttgatgcctg cggctgtttt acctgagcca gtgattttaa
     6121 aagtgacttc ttttgtaggt gtccaggtat atgctttctt gtccgcacca tgtctgactt
     6181 tatgattaaa agccgattta ctggcatgta tatgtcttcc cgataactta ggagatttcg
     6241 gatcacgccc agtcccttta gcgcgtttaa tacggtattc ctgttctaca tacacaccca
     6301 taaatcaccc aaatctgtta acaacactct tggttaacgt ttcaagtttt gaatcaattt
     6361 gcttacacag ttcaatcact tcaataacgt cttttctgaa atctggatcg ttaaccgtac
     6421 agaatctttc accttttatg ataaaatgaa tgttgcgacc aacacgatct acagcagttg
     6481 atagtttttt aagttcctta acttcatcag ggtaaagaca gacttcactc tttaacaaag
     6541 actgacgcac acgaatagca atttctctgc tcatgctcat gccgtgaaga gcagcattac
     6601 gtttaacaag ttctatttca tgagggtaaa gacgcactga taccagcttt gctggttcat
     6661 tattaactgg gatgttaaca ggcttggttt caatattaag cacgttatgc ttagcaaaag
     6721 cattgatact ggtaaatcca cttaaaatag cgttatgttc tgcaaatttt aactcatcat
     6781 cacttagcct gaacgctatc atcttcataa gtcaccccaa cagagcctgc ggcaggcaca
     6841 ggcaaaaaca tcgtttttgg acaatctgat tcaaatgtta acatttgaca tcggattgtc
     6901 tatcctgcat tgattgtacc atatgtggca ctgtgcctgg ctataaaatc gtcgctgcat
     6961 atgtccagat ttcgttgctg aaagattctt tcgtcttcga ccatcaggca aacgtatagc
     7021 tgaaagattt gtgaaagaaa aaacaggcct ggcagggctg ggaattgtta cggctgaagc
     7081 cgtaacgctt tttaagtcct cgctgcgctg cggggcttcc ggcgcaaagc gcctcccatg
     7141 cccttacagg gcataaaaac aagggcaatt aagcccctgc gcgatgattg ctgacttctt
     7201 tttccagagc cttattaaca aaggcattca acgataaatc ttcttccatc gccatttctg
     7261 ctacccgacg atgcagttct ggatcaagcc tgacgttaaa cacgccttta aacggggtat
     7321 caggttcctt tccatcctcc acacaagact gtaaatacag atcaacagat gttttaaact
     7381 cctgttccag ttcagctaat gtagaagcct cataagttac caggtcccga ataaacgcca
     7441 gttttccata caggacatta ttttcaaaat cgggttcaac cgtgcctaaa tatcctttat
     7501 attttaaatg tttcataata atccagcctc tttcagattc tgtttaattg ctttcagcgt
     7561 accacctttg atataacttt ccggatgtgg acgatgcatt aatatggtgt gattgatttc
     7621 agcattgaaa aaccgcactc ttgacccctg catttccttt ttgacatatc ccaaagagga
     7681 aaacaaaacg accagttcat cccattcaaa cgttttttta ctgtttaaaa actttgccag
     7741 tagcttatct gttttcccca taaatccatc ccctatagat atgcaactaa ttatagttac
     7801 acatggcatt tttgtcaaaa aatacaaagg ggcagaaagc cccttttttt atttaaaaca
     7861 tttcataccc ttgcaagtag tttaaccacc accggaattg atcccattcg ctaaatttca
     7921 ttgccggaat tctggtttcc cagcctttga caagggaatc agcaagctgt acgatctgcc
     7981 gcgttgacat ttaaaaacct cattcagttt tgattgtcga taacgtgatc aggttggtgg
     8041 aacaacctga tcacaccttg aatagtcagt cattaccctc cttcttttct atagttactg
     8101 tgtatccgta aagagtcaaa atgcgataag catccaacgc ctcacgctca cttttaaatg
     8161 ttctgacttc gtgtaatcct ttacctttat caagggatac acaaaactca taatcttctt
     8221 ctacttctat ctttttaatt aatttatcaa gttcggctaa tgttcgtttt ggctcaaaaa
     8281 gaaatgtgca ttgtacaatg gcgtttaaat cttcccgatc aagctctaac agagcatcat
     8341 tataagcagc tttaaaacag cgattattac gcagatcaga tgcaaactgc attgcggctt
     8401 gttttaacgt tttcatgaat ttcccttgca ttgatgttaa tagacaattt cagaatcaat
     8461 attgaaattt ccagaaaatg cagggatgcc cctgcattca taatgcttaa caaagacact
     8521 caataaaatc tttaaaactc aagcattcat cgccatcttt taatgaatca aaatattttt
     8581 catattcgtc cctgtagaaa aggactaaat caacatcact ctgcttaagt tcgccaaact
     8641 gaggaacaat tccggcttta tagtcacgaa ttttattgtt atcagtaaat atgacaacat
     8701 actgttgtcc agtatcctta tgcataagga taatagtgca aagcgttact cgttcgaatg
     8761 tgaaaacatg attagtggtt gaagtaaaaa cagatgagca tttcatttta aatctcctgc
     8821 cctgctgggc tattgtgaga ggggccattc ccctttcgat gaaacaataa tacgaccttt
     8881 aagtcgtatt tgcaagtttt ctcccgtttt tttactcgat aagtgattgt tatcacgttt
     8941 tttaatccgc gtaagcggat ggaaggggcg aacgaaaata ccagggcagc acaggagcca
     9001 catcgccccg aagcgcggcg cagccgctga cgaacgtacc agggtgcaca ggccatccct
     9061 tcaccctgca aagcccaact gaccagcagg ccatgattat gctgatttgc gccgcagaat
     9121 gccgcagtta gcgcgagcgc aagcgagttt accccgccct ggtgcattgc agcatggcgg
     9181 ggcgctgtac tgcgcgtgtg agcgtcagcg agctacgagc gcaactccag gtactaatat
     9241 aaaaccatca caggtcattt tattttgact cccttttgca gagcacgtat tcaggatgtt
     9301 ctcctgcaag taacaaaaga aactcaaact caaccagtga cagactttga ctgttttgag
     9361 tattaacttc ctcttttttc tgccaggttc ttagcccaac accataaata tgactgcatt
     9421 ccttttgtgt taatcctgct tttaaacgta atttttttac attttcaggt gtattatcgg
     9481 gtttcattta acataaactc cttaattaaa ttcttaactg gcgtctcatc gtcttccctt
     9541 aatccggcac acgtaaaatc aatatgttgt aattcattct cattcagtac agcatcatac
     9601 aatgtaattt tcgtagtttt gttcccgatt ttaacatttg ttctggcttt tatccttttt
     9661 gtaagcctgt aattgtcacc ttctttttca agaaaataag tcacatcacc tttatttttt
     9721 ctcataactg tttcaaatac cctgtaattt taacaagttg atctatactc aagccccgaa
     9781 tctgtaaatc ataactgtcg ccctgttcgg tgactttacc gttcaatccg ttttcagctt
     9841 taaagcctgt caatgcccca taccagcttt caagctggac aagttgcgat aacatacctt
     9901 tcaggatcag aatttcgtcc tttttttcca cctggtgttt ctcatactca atcgcctgta
     9961 acacctcctg cgaagcattt accagaattt ttcgctcgat atcggctgca atatcaacgg
    10021 gattccgttt tctggataac gtgcaaacat gatgcggact acgccaggaa cgactatcca
    10081 cactccccac cacagaaaag cggtcttttt ccatccgaat atgcacagag taatttttaa
    10141 actgcggtga cgtcagtttt atacgagacg aacaatcatc aagtttgttc acgcgccagc
    10201 cgttccccag gatacggcag acaacttcaa agacagggtg atattgttca aaaaaagagt
    10261 tcatctgttt ccctttttgc tacgtggctg ctgttcccgt ctttttgggt tcgatgcccc
    10321 aaaagacggg ctggctataa tatcgggggc ttctcgcccc cgttctggtt acgctctggt
    10381 ttccacgcgt tgccaccctg tcgccctgcg ctgtccggtt gtaccgtatt ttgcgcggat
    10441 ttcttctatc gtgtgttctt cacgattcca gtaactttta tgttcgtctg gacgataaaa
    10501 ccattgtttt ttctttgccg cccatttaca cccgatttct tttaaagttt ccttatgtgt
    10561 aatggtttct ccactaatcc agacccagtt acctatcact tcaaatacca gaccagataa
    10621 accagaaaga acgtttaata ctttttccag gtcatcaccg taattatatc gtgcatgttc
    10681 atcagtgctc tggaactgat ttattttatc aatatttgcc atcaatacat caaaagctgc
    10741 atttaccgct ttcatcagtt cagcccctaa cgggttacga tctggatgat attttaaagc
    10801 ggcttttctg tatgctgctt tgatatcttt ttcagttaat tcgccggata atccaaaaac
    10861 gtttaatgct tcctggatat tcattttttt aatctcctgc cctgctgggc tattgtgaga
    10921 ggggccattc ccctttcgat gaaacaataa tacgaccttt aagtcgtatt tgcaagtttt
    10981 ctcccgtttt tttactcgat aagtgattgt tatcacgttt tttaatccgc gtaagcggat
    11041 ggaaggggcg aacgaaaata ccagggcagc acaggagcca catcgccccg aagcgcggcg
    11101 cagccgctga cgaacgtacc agggtgcaca ggccatccct tcaccctgca aagcccaact
    11161 gaccagcagg ccatgattat gctgatttgc gccgcagaat gccgcagtta gcgcgagcgc
    11221 aagcgagttt accccgccct ggtgcattgc agcatggcgg ggcgctgtac tgcgcgtgtg
    11281 agcgttagcg aactacgggc gcaatgcagg agaactaaca gaacctccct aaaacgctct
    11341 gaatcgcatt ttaagcgatt tttttcattt cgcatggttt tcctcatggt tgtatcgaac
    11401 gaagcggcag agcgcgattc agggcttctt agtggaagat ccaaaaggat caaataacgg
    11461 atcatatgat ctgaatctct ggttttgaga aaggagatac cgctcgccgc agccgaacgg
    11521 caggagcgta gcgactgagt gagcgaggaa gcggaatttt tgcgatgtga cattaggatc
    11581 agtacgcacg aatattatat ttatttcaat aagttaaagt taactaggga caaatttatg
    11641 gtcttgaaaa ttgagataca taaggcatca aatatttttt gatgatttta tgttattgaa
    11701 agttatgagt tttaatgatt gatgatgttt gttttagctt gcgggtaata atcatcgggg
    11761 acagatttat ggtaaggaac catcatcgtt gctggttttg ttgagttatc cacagttcct
    11821 gaatattcgc acgcattaaa agccttatct tttcttgttt ttcttaaaaa cttaaaaact
    11881 tagagtagct ctaatttatt gattttaagc gtgttttttg tctaaacatg agagcttcct
    11941 cggttaaaca tgataacttc ctcggttaaa catgagagct tcctcggtta aacatgagag
    12001 cttcctcggt taaacatgag agcttcctcg gttaaacatg agagcttcct cggtgatatc
    12061 cacaaaatgt gtcataagaa tactaattcg ttgttctttc tggtgaatct attttgaatc
    12121 tgttgttaat ctgccttgaa tccagtgtgg attttgagca tggattacct ttgtgagata
    12181 aatcacagat atgaaacatg agaagatact cggttaaaca tgatattgat tgtgttgtaa
    12241 tatcatgttt ctggtgttaa agtctgatta tcaagagtgt tggaacaatg agaatgacga
    12301 caaataagac ttccctttct cgcttaacaa aagtgagaca tcgaaatgaa ttgaattcaa
    12361 cgctgtcaac attgcctatg gctgctaaaa aagtcttatt tttagccatg tgccagataa
    12421 actctaaaaa tgaattcgat gatgatcaca tattttatgt gacagttgcc gattacatta
    12481 aatgggttca ggttaagcct gatgcggctt atcttgcttt gagggatggc tctaatatat
    12541 tagatacgac gcttcttaag ctgaaacatg atgaaatatt agaactgagt agtgatttgg
    12601 gatttaagtt cactaaaagt aatgtacctg attcaatgaa tttgagtctg actgtttttt
    12661 ctacttatta caggaatgaa ggtagagtgg ggatcaagtt tacgaaagaa gcgaaaagat
    12721 ttttatgtaa acttattggt gaagaaaaac gttatacaac gcaagtttta ttatctgttg
    12781 ttcgactgac aagtgttaat gctgcatcac tatatcaact cattcgtaaa atatatagta
    12841 ataattctcg cgcgaatagt tttgaaatga caatagatga acttaaagat gaattgaatc
    12901 tttatacaat aggggcgggg gtgaaggatt ataagtatcc agattatcct gcttttaaaa
    12961 gagatgttct taataaatca gtaaaagaaa tcatgaaaca tacggaagta aagaacttaa
    13021 gtttcgtagt ttcagaaaaa attgggagaa aagtttataa attaaagttc tcttatacaa
    13081 ttggttatga gggcgatacc agggaagact cagagtttac taatatgttt gataaaatgt
    13141 acccacctga aaattgaggt cataacaata tgaaggataa tatcgtagat tttcaaagaa
    13201 tcagggacag catgaatgat cattcaaccg atgttgcaaa tgatgccgtt tattctgaca
    13261 aaaagaaaat atccaggttc ttttatctgc tgacacctgt cagatacctt ctggctgctt
    13321 ccctttatct ggttgttgtt attccattgg ggattcttgc gggatttcgt ttcttgttgc
    13381 agtttttagg cggtatcgca gtgatgggtt taactatcgc ctggtacatg ggtgcagatg
    13441 tcagtagtta cgttgttctt gctgcatgga tcgccttaac tgtttgtggt tgcgcagagc
    13501 agatttttga atggtggatc gaaagccgtt ttgcgttccg tctttttggt atcgggagaa
    13561 cgccggaaga atgagcgccg atcttacaga cagtaaagaa caatgcagga tagacaatcc
    13621 gatgtcaaat gttaacattt gaatcagatt gtccaaaaac gatgtttttg cctgtgcctg
    13681 ccgcaggctc ttttaaaagg aatatgaaat gatatttaac cgtaataaaa aacgagatga
    13741 acaacataat tctgctgttg ttttgccatc agctgaaaat gtattctctg attttattaa
    13801 gaacaatcca gctttgacaa ccaatgcttt tacagccatt ggggaggttg attgttttac
    13861 taaagtaccc ttactctcaa ataatgaatc agagttttta ggcattcttt cacgtaatat
    13921 tatacctgat tattccgttt ttcccaaaat acggctcatt gaatttgttc gtcctcatgg
    13981 tagcgaggaa gctgtaaaag atttaatccg tgaagtgcag aatataacct gtgattttgt
    14041 cctgaaatca tatgatgaaa cagtcctggc tgttatccag tttggtgaaa ccgcaaccgt
    14101 cagacagcaa aagaaaaaac tgattatcca gcgcgtgtgt gctatgacag gaatttcttt
    14161 tttcgaattt aaaaacactg tcgatatgat ggggagcgaa gatttttgtc agttcctgcg
    14221 ggatgatgaa gagtaattaa aaataagccc acatatttgc gggcttaaca ggctctttat
    14281 tagtacttgc tgcatttgtg attactggca acacagtttt cactgggcag tttttgcggt
    14341 cgcttttaat tatggttagt ggaactcaga caggtaactc gacctcttta catattaata
    14401 attgatagtt ttacctggct tccttcaaca tttattttgt tgaggaattt atttaaatac
    14461 gcaatatctg gagtggaacc gcatattttc atcaattctt gaccgatagg cgtaagtaaa
    14521 gccgttccta ttggtagttt ccaggttgat gaatcatcgc tttgaaactc aactgaaaaa
    14581 accgttccgt aataaccgat acgaccgttt ttacttttaa aggcgattaa acttgttcac
    14641 ccttcttaaa aaacacccac gcaagccctg ccgacatcgc ccacgcaagc atcatcgaca
    14701 ccaccgtatg gctcaaaaaa tgatcgccaa taatcatctt atagcagccc atcgcccagc
    14761 ccaatgccat caccacgatg atgatcgcac cacgacggcg gcgcaaactt ggcgcaaacg
    14821 caaaggcaaa cagcgcaaat ccgctactgg catgcgccgc aggaaagcac ttatcaggaa
    14881 tggtgttacg catactatca agcatcggca aatacggcag cgtaccatca aaaatcgtca
    14941 aatgcacagg gcagaccaca tgagtatatg ccttcaatga cgcgacaact gtcggcacta
    15001 ggatcaaagt cagcaccaga tacgcaagct cacgcacgcg aaattttgtc actgggacaa
    15061 gccacttggc agtgatagca ttgccctttt tctcatgata agcacggcaa atggtcgcaa
    15121 ccagcaagta gatcagcatc gccatcagca gcttttttag tccagaatac agtagcaaat
    15181 ctggcaaaaa agcttgcttg gcaaccatcc aatgccccat acccagataa aaccaatcag
    15241 cgaccatcag atcaatctga ctgtgctcaa gggtcagcgt ggcaacgatc gccatgacaa
    15301 gagcgatact catctcagca agtaggcgtt tatttgataa atacggcata acaaacccca
    15361 ccccaccgcc cataatacga atggagtgtg cggtgggttt ggaaaaaata cagggagaaa
    15421 tcagcggatg aatgcggtgc ggtctttgac tttgtccgcg gtgacatcaa acagctttaa
    15481 taatgtcggc gtgatcgcgt catgggtcag gacggtatcg gttgccattg gcgtgatgcc
    15541 agtttgctta tccgtccaga aaaatgcagg cacactgcgc tgttcttttg gtgcaaaggc
    15601 atttggcata ccatgtagat agacaccgtt ctcacccaga ctttcgccat gatcgctgac
    15661 atacagcatt gagacatcat aggcattgct gtgcgtctgc agccactgga tactttgagc
    15721 gatgaaatca tcggtggcaa gcaaggcatt gtcataagca ttgatcaagg actgatgttc
    15781 gcacttggca agctcattac cttcacacac tggcgtgaat ttggcaaact tttcatcata
    15841 tcgcttaaaa tacgcaggcc cgtgattgcc catttggtgc agcatgatca gcatatcttt
    15901 gccgttattg gcagcgacaa agtcatctaa gccaacgagc ataccgacat cgcggcattc
    15961 gttataagga ttggtgttgc agatggcgtt gttggtcgcg gatttataat cggcaaattg
    16021 cgcttttggc agcttatcca tcacgccttt tgagtccgaa ttattatcac gccacaagat
    16081 acttacgccc aagcgatcca gcgtatccag cacattttct tggtatttgg cggtatcgac
    16141 atcatactca tccgcgccca gatagctgaa catacacggc acagaatacg ccgtcgatgt
    16201 gccgcacgat gtgacattgc taaaattggt cacgccatcg atcttggcaa gctgtgggaa
    16261 agtatcgcgc tcatagccat tgaagctgac atgatcggcg cgtgccgtct caccgacgac
    16321 gaacaccact aggcgtggct tacgcatatc aggcttggtt gcttgtaccg cgtctttggc
    16381 gtgataaatg gtatcttttg gcgcactggc ttttttatac tcaatactgg caagcttacc
    16441 caccgagtag attggcatga tcggattgac atagctacgc agcggcttat gcacgcgaaa
    16501 gaaactggca taatgactgc tgaacgccac cacaggcagt aaaatcagcg caagacttgc
    16561 cacgatcaag cccaatcggc gcatcaaacc cttgccccaa gtcggataat ccaccttaac
    16621 aaaagccaca agcaaacttg gtagcacacc caaaccaatg atacgcatga taaacgctgc
    16681 gtttaataga tccttggtct cggcttggtc ggtctgtagg gcattttgga gcatggtcgt
    16741 atcatagacc gtgccataag tgtcagtaaa ataactggtc accgcgccca tgattaatag
    16801 caaaatcaac acaggcttta gcacatagcg atacgatgat aacagcgtgg tgatcagtag
    16861 catcgcgcca aagagcacga cagcgatcgt cagcacaaag ccgagattgt ccgcgatggg
    16921 ataggtttgg ctgattttat caaaaaaggt aagattggcg gtcgcggtca agaaaacggc
    16981 aacactcgcc acaagaacaa acggactgac cgagcgtcgg taccacacag aagtatgctg
    17041 catcatgaga aactactcaa aaaataaacg gcgggataat tgcggcaatt atactgcaat
    17101 tttttaagaa atccagtaca atttatctga tataaaaaat tatgcgatac agaaatattt
    17161 cagaaatttt attaggcaat cattgcgcaa tcccatactg aatatacact tgagagtatt
    17221 tataatttgt aaactataac caatgccttg ttgaataacg cctaaggagt ctaaaaccga
    17281 aagtttatcg aaagttatac cttcagtatc tccattctca tcggtaaata aaattggagt
    17341 gagtttgttt gcctgccaaa caaactttcc aaaataagta ataaggttcg cttcttcttt
    17401 gcttatagtg gataatagtt ttaaggttct acgactaaac gaaccttttg actttgcttc
    17461 ttcggataaa attcttcccc aaagcatttg catttgttcg tcactaatat cctcacactc
    17521 cctgaagaaa gcctctatcc aatcttcgtc aatatcagaa acattatcac tctcagatag
    17581 actttgtgct gcttgcattg ttatattttc aatgttttcc tgtcgtttcg tttcacgttt
    17641 gagaaaacgc tcaactgccc gtttttctat tccctcaagc tctaacctag aaattagctc
    17701 tgtgcgttta gcttcagctt cagccttcgc ttttttcctg attcttgtag gttcataaag
    17761 cacaccaata gcatcactta ttttttcaat aagttttgtt cctggttctg ataatcctga
    17821 taaacttaaa tcgataaggc tcatctagtg ctcctaaata cccgtgcaac aactgtacaa
    17881 gggtatttta ttgcaagagt aactaaaaat gaatcacaac tacatttaaa agtgatgaag
    17941 ttagtacatt cagataagta tctaaaagaa aatgataaag agtttattgg gccacttgct
    18001 atgattaatc atttctgtca gctctgaacc acaaattaat gagttcgttt tcgtccagat
    18061 cctgataagc cagtaatgct gctttgatta tttttgtctt gggacgatgc agtctttttg
    18121 attcgttatc aatgaattca agcaattcat tcggtatgcg gaatgaagcc agttttgttt
    18181 tgttctctgt ggggcgtttt cctgcgtcct gaacaaattt tgtggcgttc acttcctggt
    18241 tttgttctag ttctggtagt tccagctttg ccatgatgta ccttctaatc tgattgtttc
    18301 acactaatgt atatcatgat gatgtacatt agtgtatgtc actatgattt acagttccag
    18361 ttctttcagc aagagttcga tttgtgcttt tgcctggctg gcaccgctaa cattactttt
    18421 taactcgtga acccccattc cctgattcat tgctcttaca aaggcccgga gaaaagaaac
    18481 cgtgcttttc attggtttaa gccagtaatc atggctgttg agagagtctc ttaattcttg
    18541 ttgttcactg aacgtatttg gctggcattt atacaggagc acatgtgcag ccagcctggg
    18601 atttattttt tgcgcatccc tgacaatttt tgtcacagtc tgaagactgt ctgcttccac
    18661 ctgggcaagc gggtcgatcg ggacaatcac aatgtctgca acttttagtg cagttctgaa
    18721 ctccgtgctg tcatatccgg cagtatctat aatgaggatt tctgctacat ctgccatttt
    18781 tgttattttg gcactgatgt cgccataggc ttcatttaat gcgacgggcg gtaatccggc
    18841 ctgttctctg cgtttattcc attttttggt cgagccattc aggtctgttt caacaagccc
    18901 cgttctcttt ccctgttgtg agaggcaaac agttaagttg gttgccagat gtgatttacc
    18961 tgtaccgcct ttatcaccac cgattacgat aatcatagta ccccttgtat gtcacagtga
    19021 tttactcatg tgtatttcta tgtgatgtac gcatgcgtat gtcacatgga catactatag
    19081 tgtatgtcac aatgaattac aatgatgtac atcatgaaga aatacattat tgtatgtcag
    19141 tgtgatgtac ccgttggttg gtcacttgaa agcaaaagtg acagcgttac tttcggtatc
    19201 ctcccttgct gcgaaacctt gtggcaatac agcaatgagt atgtttagtt acaacattca
    19261 aaattaagcg attagattcc gcccgtacgg gcggggcgcg ccactttctg tagtggggag
    19321 gggcagggag gttatgtttg ctggatgagc ggaggatgga atgagctatt agaatgttgt
    19381 aactaaaggg tatcccaatt aaaggccact tacgcggcct ttgtctgtcg gttcttcggt
    19441 ctggtgacat accacgataa acatcccaca gcaacactca taagcattcc ggataagcga
    19501 tcatcgatgc caggaaacag gcaaccgaca aacatcgcaa cagggaaagg aagaagtaaa
    19561 cagcgcccca aaatgctcat aaaatccata aatcctcctt ttttgttaaa gggcaggaag
    19621 cggttcactt cctgccctta gttacgtttc cgttgtcatc aatcgatgaa ttggcggatc
    19681 agttccagaa ctgcaatcag cgcgtttgca acggcgacca cagctctgag aacgatgatt
    19741 aacatattaa tcatccgttt ctccagtaaa actggaggaa atcaccgttg cttgctcata
    19801 cactgcctgt cacaacggct ctctcgcatt gttaggtgta gagtgacctc ctcctgccag
    19861 agcaccgaat acagcaccac ctgtttatcc agccaggact ttaaaatcgt tcatacccgc
    19921 agatacggga acagcaggat tgcaagccaa ctacatgact aactgactga ttatacgcta
    19981 cacaaaacac ccgttacacc actaaatgtg gttccttaac cacatttagt tgctttacag
    20041 caccatagtg aataaaatgt aaacgttagg tgtagagtaa ccttctccgg tcagagccac
    20101 caactcaaga ctggaaaacg caaaaagccc gattaccagt cgggtttttt gtgcctgtcg
    20161 ttcggctgta gttccgtttt ttctccttca tttgagggat ttttctttca tttttttgtt
    20221 gcttccaata ctgcctgtcg cgttggtaac agcttgacgc gttaccggca ttttcattac
    20281 tcaatatttt tcagtgaatt aaaatgcaac agcgacagaa aaggacattc agctttttag
    20341 atgaatgcta tagcaacggt gtgctacgta aaatctgcga aaaaagcagg ggtagggtag
    20401 gttatttttc acgtagcaac gccatctggc actgtacata ggtatgtcac acagacatac
    20461 ctatgtgtat gtctgtgtga ggcactgttg caaagttagc gatgaggcag ccttttgtct
    20521 tattcaaagg ccttacattt caaaaactct gcttaccagg cgcatttcgc ccaggggatc
    20581 accataataa aatgctgagg cctggccttt gcgtagtgca cgcatcacct caataccttt
    20641 gatggtggcg taagccgtct tcatggattt aaatcccagc gtggcgccga ttatccgttt
    20701 cagtttgcca tgatcgcatt caatcacgtt gttccggtac ttaatctgtc ggtgttcaac
    20761 gtcagacggg caccggcctt cgcgtttgag cagagcaagc gcgcgaccat aggcgggcgc
    20821 tttatccgtg ttgatgaatc gcgggatctg ccacttcttc acgttgttga ggattttacc
    20881 cagaaaccgg tatgcagctt tgctgttacg acgggaggag agataaaaat cgacagtgcg
    20941 gccccggctg tcgacggccc ggtacagata cgcccagcgg ccattgacct tcacgtaggt
    21001 ttcatccatg tgccacgggc aaagatcgga agggttacgc cagtaccagc gcagccgttt
    21061 ttccatttca ggcgcataac gctgaaccca gcggtaaatc gtggagtgat cgacattcac
    21121 tccgcgttca gccagcatct cctgcagctc acggtaactg atgccgtatt tgcagtacca
    21181 gcgtacggcc cacagaatga tgtcacgctg aaaatgccgg cctttgaatg ggttcatgtg
    21241 cagctccatc agcaaaaggg gatgataagt ttatcaccac cgactatttg caacagtgcc
    21301 ctgtgtgatg tatacttggc gggttattct gcctgttccg gcttagtttt ctcaatgagg
    21361 aaatcttcca tgaaatgccc ctcatcaagc agcttctgta aagcaagcgg acgcttgcca
    21421 ttaccagacc attctctgat ctcgccattt tcatccttaa agcggtattt agcctttctt
    21481 actccgcctt tggctttccg cctggttttt actgtatctg ctgttacagg agcaaggagt
    21541 gattcgggat caagacctaa agatatgatg tattcaatgg cctgctgacg tttggcttca
    21601 agttcctggc gttcagccgc ttcacgttca gcatcttcac gacgttcttc aataacaacg
    21661 ttgagctttt cctgcatttc gagcaactgc tcaaaatcaa tctcacgggc aaaaacacgc
    21721 aatgaacgaa tattcatcat agtacggctg ataatgccgt attcatcttc tttagtcagt
    21781 tcgctcatat aattaatcct cttatttagt gtacgtactt ccagacgccg gaaggcacct
    21841 tatcataaag tttattcatc accagttctg ctcggcgatg atctgcggca gaataaaatg
    21901 cctctgattc tgataaaggc agtttgtaac ggttaatctc aataactttt tcaagagtat
    21961 ctcttgatgt acattttctt aactgaaaaa gccattcctg ctttgttttc acttcttagc
    22021 ccttgccgga aaacccagct tccctgtttt cttatcctca aggacaagaa ctgtatcata
    22081 cgttccacca cttttctttt taaatccctt aatcaaatcg gttttccctg atttaaccag
    22141 tttttcggcc tgtgcctggg tgattttctt accagaaaac tcactccaga ttttaaattc
    22201 acatccggta cagaaataac ctttcggcct gataacaata tgtttaccac aggaagggca
    22261 gggagagtcc ggcgttgaaa ctgccctgct ggcgcagcgg gttcaatctt catctcgccc
    22321 agtcaacatc agaaatcatg cctgtcagtt caccgtacag atcattaata aactgttcaa
    22381 ccgtcaggtc gccattttct atggcagcct gcttttcaga ccataatgct gtcatatcag
    22441 gattaaccgc tataccgtgc agggcatcaa taagcgcata tccggtatca gtaggaataa
    22501 gtttcccttt ttccagcgtg atatagtttc ttttcttcag cgtttccaga atggctgcgc
    22561 gggtagctgg cgtaccaatg ccaccatgtt catctttttg tctttatcct tatccttcaa
    22621 caatttttta attgttggat cagtgacaaa atccgcgaca cgaacaagcg cagcaagcaa
    22681 ggaggcttcg gtgaataacg gcggcggtgt tgttttcttc tcattaacaa taacttcttt
    22741 tgtcgtcagt gtttctcctg tgcgaatttt acagagcagt tcaaaagcgg aatcatcatt
    22801 atcttctgat tcaccttcgt ctgtggtttc cgcgccaaga aacgcctcaa atccgctgtc
    22861 agttgttttt ctggcacggg catagaacga ctcatcacca cactgaatgg caaccgatac
    22921 ttcctggtat gctttttcag gcatgaactg aacaagatag tgttgtgcga tcgccaggta
    22981 aacattgcgc tcgtcggtgc tgagtgcgtt aacatcaggc acactggcgg tcgggattat
    23041 cgcagtatgc gcagtcacct ttgcactgtt aaacgctttg cttttacgtg cagaatcaat
    23101 atccagcgac tgaggaaaga ctgatttcag ggcatcgata acctgcggcg cttcgctgaa
    23161 ttgttcatct gaaagatatg agcagtcaga gcggttatac gtaattgctt tatatttttc
    23221 gcgtagttgt tgcgtaatat ccagcgtttt ttgtgccgtc attttaaact tcttgttcat
    23281 gtattgctgg agtctgacca gattaaacgg caacggcgca gccgtttttt tatcatcagt
    23341 tgctgctgct tcaactgtag ccggttttcc tgcaagggat gcagccgttc cgtctgccca
    23401 cgctttatca agtaatttac ggtctgtcag cggagcaaat tcccctggct tccagttcgc
    23461 cctgataaca tcagcaccac gctgaaagac tccggtcatg gtgtagtaaa aactggattt
    23521 atggttctgg ttagcacggg tacgattcac aatcaggcca agaacgggag tctggacgcg
    23581 cccgacagac agaacgccct gatatcctct ggcttttgcc ggaatggtgt acgcacgtgt
    23641 catagagaag ccatagacgg catcggcaac tgaccgcgcc aacgccttaa ggtaaagtcc
    23701 tttgaaatca cgattgtctt taagatttgc cagtgccttt ttcactgccg gaagcgtgtt
    23761 gtcgttaatc agaacgcgct ttacgggttt tgtgttgcct gcatattccg tgacttcatc
    23821 aaccagtagc tgtccctcat catcaggatc gccagcgtga acaatttcag tcacgtctcc
    23881 acgtctgata agttcgagaa tcgttttaac ctgttttgct gcactttcaa caggctgata
    23941 cttcacggga taaagacgta aaggaagcgt ttcaaccttc caggctttgt attcaggatt
    24001 atagttttcc ggcggttgtg attcgataat atgaccaaaa cagttagtca caatggcgtt
    24061 atcactttca aaccagccat catggcgggt aaaattgcca ccaagtgcct taacaatatc
    24121 atttgctact gcgggttttt ctgcgataaa aagtctcatt ttgttatcct ttacattgaa
    24181 ttaatctttt ggcacaacgg atagcgcgtt cttccgatac tgtatgttgc cagttatctt
    24241 tacttactac tctgaatctg cgctcacagc cattaaaggc aaaaattccc tcacggttaa
    24301 gtatattagt tgcaatatcc cactctttaa ttctcaaaaa ggctgttaca gcaaaaccag
    24361 aatacgaagc aatttttgaa atgatgcatt catcagcttt tattaacgcg gcaaaaggaa
    24421 ctatcttttg atgaccacac gaaatcagag ggagtgtgtg aatatcataa taagctattg
    24481 aatgacaatc catgcgggta tcatcaatag ctgccagcca gaatcctgcc tggtctttta
    24541 tttttctgct aatatctgaa tctgaaacac cagggtttaa taaatatcca ccataacaga
    24601 tatggaaggg gtgagcagat tcatcaatat caggaagttc ttttaccgga ttttctttcc
    24661 agtccttcag aacaccgaat tcaagagcat ccagcaaaaa ctttcgttca aatccatcaa
    24721 acaatgcata ttgcatgtgt taatcctcca ggtaacttaa atagtcaggt tgtgccccgt
    24781 tctttttata aagttcttcc ctttcctcta tatcttcatc tgcttcctga acgctcagat
    24841 cgagggttcc tttcaaaaag gcacgagcat tttttattac atccacttat gcactaccag
    24901 cagataagca caataaaata gctataatag atttggttac tttcatttct aacctcagtg
    24961 attgtaacta atcatcacat ttcgttggcg cgaaatgtga tacttgcagt accctgatac
    25021 ccgttggcgc ggttatcagg ttttaattaa cctctgacac gaccaaactt actcatgatt
    25081 tttgatatag cagcaggatc agccgttgaa cactgattca gaaatgcttt acgggcatca
    25141 aaagttttac tccagcgaat gcttcctttt ttctttttca gaatattaaa aaaatccttt
    25201 tcagcacttc tgcattcact ccctccacct tgcccgactg cttttccata taagcaaagt
    25261 accgaagcgc aagggtcaga tgcattagca tttgttgata ttaatcctaa cgccagcaag
    25321 gctggtaaaa ccactctatt ttttccagtc atttttcacc tcattttgtg ctttaatgaa
    25381 aactattttt acctgttcag gtaaaaaact tttctcgttt cgccattcca cgactgctgt
    25441 tgccccttcc atcttcaaca gatctctgat tcgaaaccac agcccagcat catgtgttgt
    25501 cactacaatc ctgtccgcat gagccagagc gtaataaaac gtcggcgttc tggcctgtac
    25561 tccggataag gaagtgatct cataaaccca tcctttaccg ctaacgtctg actttatgac
    25621 accgtgaaaa tcggggacat atggcaatga gggattgatc acttcatttg ccttttcaaa
    25681 ctgcacgggt gttggctctg gcggtgatga acaaccagca agaatggaaa tcaataaaaa
    25741 cactaacttc ttcatcatgt tctcctttat gccgctttat cgagatcagg aacatctttt
    25801 actaccagat acccttttgc gacgatttca ttttcaaata ctttctgtgg aggcataacc
    25861 agctttcctt tcttcctggg gaaaatgttt gcaagttccg gagcaacctt cacaaagtca
    25921 ccaaagaaat actcattgag atagtagaat gccttttcac aacgaatcgg gttttctccc
    25981 ttcattgcca ggatttcttc actaaatttc agtgtaagta gttcatttgt tgttaccagt
    26041 ggacgctcaa taaggctttc tgattcagat ctcgatgttc ctccacgact acgacttttg
    26101 cttttcgaac cgatagttac tgttcgtgtc cccatcgctt ctgataactc ccgcgcatct
    26161 ttaacatcag aagcggaata aacaattttg cagggtgcac ttgccagtaa tgttttactg
    26221 cctgcataac tgtagatttc ctcaagctga gagccgacct ggtaaacaat caacaatttc
    26281 agcttgtaac cagcaatgag accagattta ctcttgatgt aatctacagc accaattgac
    26341 gggaattcat ccagtagaaa aaggcagtca tgctttattt ccggatcatc cgttggcatc
    26401 ttttcaagat tcacctgtat agcaagctga aagaaaatat ttgttatttt ttcagccaac
    26461 ctggcattat caccagaaat acagaaataa attgtcatcc gctttttccg cacctgccgc
    26521 aaatcaaaat catttccatc agtagcattt gccacattgg ggttagcaaa cggtgataat
    26581 ccattaagaa agttgctttt gagttgtgct tttgttttat cctccatctc aatgaatgcc
    26641 ttaaggccag cgacaccaat atcagcaagc gttttgtctt tcccccttaa ttctgaacga
    26701 atatcattca tgaatgaaag aagatgttcg ggttctgcac gctcaaaaag ctgattaatt
    26761 ttagacattg agaagacagg ctcaatatca agcgttttta gtccagcctt atcattctta
    26821 atcataaacc ataaagcgac agcataagat ttaaaaacac cacccgccgt cttattaaga
    26881 tagctaccag catccgcacc atctgtctta taggggtaaa taatcaacga aagattttca
    26941 agctgattaa aaccttcact cgttcccatg ctgacataat acaaagggtt aaagcggtgc
    27001 gttaccgaac taaagggatc aaatttataa acctcatgac ctaaaatcac cttcctgata
    27061 agactggtga tattaaaaca ctcccttttt ggatcgagaa taataacact ccaccagtac
    27121 ttcataagat tagtgatgac tataccagca cctttaccag cccttgttcc ggctcccata
    27181 gcactgaatg ctgctcccac atagcgtatg attttcttgt cttttatacc aacgataatc
    27241 ccgtcttttt cgtcaccgtc aagcgtgaaa ctgttagact cacgaatatc attaattgtt
    27301 gcaaatcgcg catcaccgaa aatattttct ttcttctcgt tcagttttat caacgccgca
    27361 acagggatga taagagacac gacaaatcct gctccaagtg caacaaaggc taaaactctg
    27421 atatccgtag tcacaacaga taaagtaagg ctgtctgtaa aaatatccat cctgtacgca
    27481 tcaatggatc tgaacacagc cttcaatggt ttcagattct ttatctggct gaagtaaagc
    27541 acaaagaaaa taaatgaacc tgcaaaccat gccgcaacaa gcataacagc gaatataaaa
    27601 acagattgcg acttattaaa agttttcaca ttcagcccct ttgtaataga tgtcgctcat
    27661 atgcctgaca acaccatccc tgcctacatg aacaacaata tcaatactgt ccataatgat
    27721 tttacgaaga acatcaaaag gaagattctg acattctctg ttctgataac attttgtcac
    27781 catctgaatg atggcatctt ttgccgaggc cgcgtgaatg gacgtcatgc ttccactgtg
    27841 accagaacca gtaactttca caaaatccca ggcatcgcca cctttaattt ccgccagtaa
    27901 aattctgtca ggtttcatcc tcaaacagga tttgattaat ttcgctgctg ttatgataga
    27961 gccggaatca ctgccggatt ccgaagggta aaaaagatgc acataatttt tatggataaa
    28021 aaatttgatt tcctctgcat cttcaatcgt aatcagccgt gtattcaatg gtatgaaatc
    28081 gataagactc ttcatataag ttgttttacc ggaacccgtt gctccggcaa atacaatggt
    28141 tttccctgct gctacggcaa gctccataaa agcagcgata tttctcgctc tgaataaatc
    28201 aagaagctca tcatcgtatg tcctgtgttt ttttcctgac tggacataat cataaaaacc
    28261 gttcgccaca tattctttat gtgttatctg ccgtgtcgat ggtttacgta tagtgattga
    28321 aatagtattt ctctcacatg caggaggaaa aacgacctgt acacgttctc ctgattccag
    28381 tgttgctgaa agtaatggct tggtctcgcc gatttcatct ccccgaaatg atgccagcgt
    28441 atgagaaaaa cgtttacaaa aatcaaaggt cacactgtca ttattatgga atgtccagtc
    28501 accattaatt tccgtccaga tttcaccagg acggttgaca gcaatttctg ttagcccttc
    28561 aatatcaaga aaagactgga atacttgatt tttataaaaa tcgagcgatt tattgctaat
    28621 gctgacagga ataacgccac tcatactatt ttacctttaa ctcatagata tcagaaaaat
    28681 caatgtcttc cccgaccata atagtgatta tgtcaccctg gtttttatac attgttggag
    28741 gaatatttat cgaattctca agtgcgactt tagccatttc agccattgcc tgtcgtgagt
    28801 tttcggtata atccacatta cgatctttac caggtgcatt atccgccgcc gccgccgcta
    28861 catcctgaac catactgagc ataagagaac caccaaaacg ctcccagaag tgagaatcga
    28921 tccatccatc aatgcccgct tctcccaggg gaccagctgc tgctgtattt accatatcga
    28981 ttcgtttatt atcaggcgtc cgcaactgct cccacataac gaacattctg ccctgtccat
    29041 gattcagcgt tccggtctgg taacgaccaa aagccttagt tccctgttcc agtaattttg
    29101 tgtggtgatt agcactccat acatcttctg taaaaacaca actgatacga cctgcaacat
    29161 cagaaacaaa gcgtgtggtt aaagcacacg gtataagcct gttttcctca atatagagat
    29221 caggatcgag attaatcgca gttactttca ttcctgattt accaggcaca ttttcttttg
    29281 attctttcct ttcttcctgt ggctctgatg tctgcgatgt cctgctgctg ccactcgcgc
    29341 cagtctgact ccctccctga ccagcaacag gtattgacaa atatttcctg aacccctcct
    29401 gtggcacttc tttatgaccg tcagaaacag tattaccaga accagtagcg cctggattga
    29461 taattgcttt gttctcaaca gggtcaaaat tctgaccaag accagtacgg attttactta
    29521 cctgagaaat gctggtatcg cctgttgatt ctgatgcttt ctgctggtca tctgcaaaat
    29581 aatttttata tgttttcagc gcaaaaatac caataactaa tacagccatc agaccaaaca
    29641 gaaagccaga acgcatacga ttatttttgt tcctgatatc accgatatca tgaacgggtt
    29701 tactttccag ctttgactga ttcgcatcat caactaaatc atctgttttt ttcatatcag
    29761 tcattattca cctcaacacg ttctaccgca ggagaagatg tattcctctg gtcggttaat
    29821 acttttccga aagatttatt cacaacacca acaacgctgt ttccatagcg taaaacaaat
    29881 ttgtcattaa cgttgtggat aaccataatt ttatacttgc cttttgtttc cacattaaaa
    29941 gcaagtgtct gttcctgacc atctcttaca gcaaatggag ctgggaatac tttccctgag
    30001 ttaaaaccta aaaaggtaaa tatgccatta tcataagcaa aatcaggggc gatacgacga
    30061 ctgtcatatt tttcattgac tcgcatgaaa taattccagt ttttaggcgc atcggctttt
    30121 tcaaaacttt cagaaatcag tttcttctgt ctgagttttt cctgtttttc ttttgccagc
    30181 cttacttctt ctgcattctt tttcgctatt tctgatggat agcggaattg tacaacataa
    30241 gcaggttgcg cttgtttttc ttctgacaga aggattagat ccagactgta aatccgcttt
    30301 gttgttctga caaaaagatt cgtatcccat tccttttctg ttggctggaa aacctttttc
    30361 agtttttcgc cttcatcgcc ttcctgttcc tgaacaacgg gacgcggatt gatgtaaaca
    30421 acattatcat cggttgtgat ttcccatccg gcaggaaaac cagcctttgc actgataaca
    30481 gcctcacctt catcaaaaac aactgatgtg agaaatcctg tctttgaccg gataacggtc
    30541 gtattcgcac cgttataact cacagtctgc atccttgcat caaatccaga aggtaacgga
    30601 gtcgctgctg aatacacaag cccaggaagc aataaacatg tagcaataaa taatttcttc
    30661 atcattcccc cctgacttcc cgatctgtct ggtaactggt tacagtaaaa ccgagaggat
    30721 taagctcacg actggaactt ttaaccgact tttctggttc aaaacgatat gtcacacgag
    30781 ccgtccagta tgaaactgaa acctgtccgg tcgtgaagtt tctcgtcgtt tttttaaagc
    30841 gcacagaggc cagtttatct ggcgcggttg cgtcagaaat aatattggag atgacctcaa
    30901 ccgcaacgta agaaccgtta ttatgataaa ttttatcagg agccatatcg ctgttataca
    30961 gacgaagata atcgtccttt actgtattcg agctatatag ctgtacagtc tcataatcat
    31021 actgaagaga cggataatta taaccttcac gtaaagtaat atacgtgttt acaaaatacc
    31081 gcccaagtgc ttcagattgc aggatctttt cctgtttaac ctgagtaatc agttctgtac
    31141 ggcctgtttt ttcgccaaca gccacaatag caagctcttt ttcttttaat ggcacaagaa
    31201 cacaaattgc agccccaaga cataaggaca gtaatgatgc tgccactgca acacgccatg
    31261 catttttctt agaccgttca ttttctgcat agagttgtct ttcgaaggat ttagctgtct
    31321 caatcttctt ttcgattttt tcactcataa ctgattacct ggctgtggtt aatgggttcc
    31381 agttcgccgg aaacatcaga aggaaggtta ttccttgtgg tacatccgga aatctgaaac
    31441 aggaaaaaca aaagaatcac acatcgcata aattcacctt attaagcgtt cacgaacacc
    31501 tttaagagat aaaggccaag atgaagaaaa agaatcccac caataagaag caaaaagcga
    31561 agatccatac cctttctcca tttccccgca agccctaaac caagtcttat tcctgcgaag
    31621 caggtaaaca ctcctaaaag aaggattagg acaaaataaa gtatcatgac aaaataatga
    31681 agcatgtcac aaccagctct gactgattcc acctaagacc ttaaataagg taattcccgt
    31741 gattaaaata acaagctggg ttattagctt tctcttcctg ctccatccgg aggtgatatg
    31801 tttatcaacg aacaccagta tccagttcag ggacatcaca taaaaaaaac gtttcattat
    31861 tttcatgcgg cgttcctcgc tcgtgctttt tccagggatg caagtcttct cgccttcatc
    31921 cccaaagcac ctgtcggagc cagcgcctga tatccctttt cacccgccca ttcagcggct
    31981 gttcgcgcac cacgccccat cagattccct gctttccctg ataaagaatt catgcccgtt
    32041 cttccggcca tccctaagcc aaaacctaaa ccagcacgac cgccaagcat cagagactta
    32101 ccagtagcca taccagcagc acccagcccc atcatggctg cgccctgaac agcaccttca
    32161 acacctgcac ctgctaactg gacagcaaaa cctttagcta taagaaccaa taaagccccc
    32221 aaaaacccag ccataaagcc catagcacct gtagatacaa gatttacatt ccctgtttgt
    32281 gcagcacgaa tagcatggga aaggatcatc ccttgaaaat ccattgctat ccgaatcact
    32341 aaactggcaa atagaactgt cagaatactg gagaataacg attgtaacca attattaaac
    32401 atgctccgaa taaaaccaaa catgagacaa aagataaata tcggtgctgt aataatcagg
    32461 agtttcatcg ttacatctgc tgttaaaaaa acaaccacac taacagccat taaaataagc
    32521 gagccagtcc atattaaaaa ctgaccaact acaccttgat ctttaacata tgttgattta
    32581 tctaaagagt atatttccgc accaagattt tgcgtacttt tccataagtt atctaaagtt
    32641 tgccagacac tcacccctcc agaaaaacca tctttcatgc cttgcaacgc atcagtagcc
    32701 gcagttaaat acccatccgc attagttata aacatgatta tgattgcaaa ttttgataga
    32761 tcccatacta aatcctgcaa gggcgtctga gttttactgg caagtatttg atatcctttc
    32821 catagtacat ataacgtaac cgataccgtt gctaacgaca taatatcatt agcaaattgt
    32881 gtttgataac tggagctgat tttatctacc gaatccataa cggtagaatt atatttaacg
    32941 aaaaacccct gagccatata tccacctttt aatcaaaagt taatggttca gcttctttct
    33001 gctgtttaat aaaggcctct tgttcttgtg tgaccaacat ctcatgatct gctttatttt
    33061 tattattcca gacatcatac tgcattttta tcgcctgcat ttttaacgac tgcgcctgta
    33121 atgcgttagc caaatcctgg gattctttga tatcctttga agcctcaatt cggctactta
    33181 aactctgaat ctgagacatt gcacttgata actgcttatt gatctcattt ccctgttcaa
    33241 tactggcagc cgttgacaca atccgtgctt tacagagatt atcattac
//
