LOCUS       NZ_CP069690             3191 bp    DNA     circular CON 02-DEC-2023
DEFINITION  Escherichia coli O176:H45 strain MIN9 plasmid pMUB-MIN9-7, complete
            sequence.
ACCESSION   NZ_CP069690
VERSION     NZ_CP069690.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN17831482
            Assembly: GCF_017570665.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli O176:H45
  ORGANISM  Escherichia coli O176:H45
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3191)
  AUTHORS   Majewski,P.
  TITLE     Plasmid mediated mcr-1.1 colistin-resistance in clinical
            extraintestinal Escherichia coli strains isolated in Poland
  JOURNAL   Front Microbiol (2021) In press
REFERENCE   2  (bases 1 to 3191)
  AUTHORS   Majewski,P.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2021) Department of Microbiological Diagnostics
            and Infectious Immunology, Medical University of Bialystok,
            Waszyngtona 15A, Bialystok 15-269, Poland
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: Unicycler hybrid assembly v. v0.4.8-beta
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100x
            Sequencing Technology :: Illumina MiSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 12/02/2023 11:40:59
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,984
            CDSs (total)                      :: 4,862
            Genes (coding)                    :: 4,587
            CDSs (with protein)               :: 4,587
            Genes (RNA)                       :: 122
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 15
            Pseudo Genes (total)              :: 275
            CDSs (without protein)            :: 275
            Pseudo Genes (ambiguous residues) :: 0 of 275
            Pseudo Genes (frameshifted)       :: 81 of 275
            Pseudo Genes (incomplete)         :: 185 of 275
            Pseudo Genes (internal stop)      :: 48 of 275
            Pseudo Genes (multiple problems)  :: 37 of 275
            Pseudo Genes (short protein)      :: 3 of 275
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP069690.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3191
                     /organism="Escherichia coli O176:H45"
                     /mol_type="genomic DNA"
                     /strain="MIN9"
                     /serotype="O176:H45"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:2810408"
                     /plasmid="pMUB-MIN9-7"
                     /geo_loc_name="Poland: Bialystok"
                     /lat_lon="53.12523570 N 23.15905989 E"
                     /collection_date="2018-03-05"
                     /genotype="ST-6856"
     gene            join(3167..3191,1..89)
                     /locus_tag="JSU11_RS25095"
     CDS             join(3167..3191,1..89)
                     /locus_tag="JSU11_RS25095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032667588.1"
                     /translation="MHEPPVQSDCCALSGNYHLESNPERHDLSPLAAATGN"
                     /name="hypothetical protein"
     gene            <344..499
                     /locus_tag="JSU11_RS24535"
                     /old_locus_tag="JSU11_24540"
                     /pseudo=""
     CDS             <344..499
                     /locus_tag="JSU11_RS24535"
                     /old_locus_tag="JSU11_24540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707212.3"
                     /GO_function="GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /name="helix-turn-helix domain-containing protein"
     gene            593..1237
                     /locus_tag="JSU11_RS24540"
                     /old_locus_tag="JSU11_24545"
     CDS             593..1237
                     /locus_tag="JSU11_RS24540"
                     /old_locus_tag="JSU11_24545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017900990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolone resistance pentapeptide repeat protein
                     QnrB19"
                     /protein_id="WP_012954666.1"
                     /translation="MTLALVGEKIDRNRFTGEKVENSTFFNCDFSGADLSGTEFIGCQF
                     YDRESQKGCNFSRAMLKDAIFKSCDLSMADFRNVSALGIEIRHCRAQGADFRGASFMNM
                     ITTRTWFCSAYITNTNLSYANFSKVVLEKCELWENRWMGTQVLGATFSGSDLSGGEFST
                     FDWRAANFTHCDLTNSELGDLDIRGVDLQGVKLDSYQASLLMERLGIAVIG"
                     /name="quinolone resistance pentapeptide repeat protein
                     QnrB19"
     gene            complement(2448..2597)
                     /locus_tag="JSU11_RS24545"
                     /old_locus_tag="JSU11_24550"
     CDS             complement(2448..2597)
                     /locus_tag="JSU11_RS24545"
                     /old_locus_tag="JSU11_24550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017880354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_162846302.1"
                     /translation="MPSEYDEQRGSTDTQLPGEYDLFDLMAKVTEEILHAPVDSGPPVG
                     REAL"
                     /name="hypothetical protein"
     ARG             594..1234
                     /name="qnrB19"
                     /gene="qnrB19"
                     /gene_name="quinolone resistance pentapeptide repeat
                     protein QnrB19"
                     /tool="amrfinderplus"
                     /drug_class="QUINOLONE"
                     /identity="100.0"
                     /coverage="100.0"
     Typing          complement(2633..2806)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="2.5600000000000002e-86"
     Typing          2..89
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="89.888"
                     /coverage="94.0"
                     /evalue="2.35e-23"
     Typing          2905..3191
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="90.0"
                     /coverage="91.0"
                     /evalue="8.460000000000001e-100"
ORIGIN
        1 actgctgcgc cttatccggt aactatcatc ttgagtccaa cccggaaaga cacgacttat
       61 cgccactggc agcagccact ggtaactgat ttagaggagt tagtcttgaa gttatgcgcc
      121 tgttaaggct aaactgaaag aacagatttg gttactgcgc tcctcgtaag ccagttacca
      181 cggttcaaag agttgctgct ctgagaacct tcgaaaaacc gccttgcagg gcggtttttt
      241 cgtttacaga gcaagagatt acgacgccac agaaaggatc tcaagaagat ccttcaccgt
      301 aatggaaatc tatccaatcc gagcacatag gatacacatc gttataacgc tggatctgcg
      361 tgaattccag cagcagcaag agaaagactt tctacaaacc agcttacagc aggctaaatt
      421 taaccagaaa aaagccgctg aattactggg tctgacttat catcagctac gtgccttgct
      481 gaagaaacac cagatctaat acgcatttgc aggtgtcata ctggcgggtt tgacgcataa
      541 cgtcataagg tttaccatgg cgtcattact gtataaaaac acaggcatag atatgactct
      601 ggcattagtt ggcgaaaaaa ttgacagaaa tcgcttcacc ggtgagaaag ttgaaaatag
      661 tacatttttt aactgcgatt tttcaggtgc cgacctgagc ggcactgaat ttatcggctg
      721 ccagttctat gatcgcgaaa gtcagaaagg gtgcaatttt agtcgcgcaa tgctgaaaga
      781 tgccattttc aaaagctgtg atttatcaat ggcagatttc cgcaacgtca gtgccttggg
      841 cattgaaatt cgccactgcc gcgcacaagg cgcagatttc cgcggtgcaa gctttatgaa
      901 tatgatcacc acgcgcacct ggttttgcag cgcatatatc actaatacta atctaagcta
      961 cgccaatttt tcgaaagtcg tgttggaaaa gtgtgagctg tgggaaaacc gctggatggg
     1021 gactcaggta ctgggtgcga cgttcagtgg ttcagatctc tccggcggcg agttttcgac
     1081 tttcgactgg cgagcagcaa acttcacaca ttgcgatctg accaattcgg agttaggtga
     1141 cttagatatt cggggtgttg atttacaagg cgttaagtta gacagctacc aggcatcgtt
     1201 gctcatggag cggcttggca tcgctgtgat tggttagttc agcgtttaca ccgggagatg
     1261 cgtttgacga tttactttta accagtacga ctcctggcaa gcattgtgaa acgttttctc
     1321 cagtggacga atatcctgac ctaccgcatc aacgacatgc catctaatgc tgcattctgg
     1381 acaggaattt gatgaatatc taaccaattc attttcgcga gaacaacgaa taaccactcc
     1441 cgaatcatga agccatgtct tatttataat ctcaacttca gccatgtcat cccgtttact
     1501 gacatcctca atacaaatac cccgcgatca tcgcgtttat tgagtttatt atttatctat
     1561 gcgattccga tgaaaaattc gcacgaaaat atggctaact catagcaaaa aaaaagccct
     1621 tagggggctt ttttttgtac ctgacggtca gaccgattaa acataacatc ccttgcgtgc
     1681 accgcctgag aggctctgag ggcgtttctg acgggaaacc ggtaaaacag gcaacaaacc
     1741 acccgaaaac ccgccagaaa cgcgcaggct ctgtttttac ggcatgcatg agtatgcgcg
     1801 caagtgcata gcgcggaaat tcacaccatt cagccagggg aagtcctgtt tccggcgttc
     1861 ttcccatccg aaaacggtga acggggacgc aatttattgc gtggccgtga agcgttgccg
     1921 gcgcagccgg aaaacccgct gtcagcacgt tcaggaaggg ggtgatgtac aaatggcaca
     1981 gaggatgaaa gtgacgtatg agctgaagga atgatgtaaa agtgatgtat aaatgatgta
     2041 aaagtgatgc aaaagtgttg taaaagtgat gtacaggccg aaggagtgtt gtaaaagtgt
     2101 tgtaaaagtg atgtatgaac tgaaggagcg atgtaattct gtagtcagga agaataaccc
     2161 gttcatgcag gcatgaacga tacagtgagg aacgcttttt tttgtcacga tctcccctta
     2221 acctgcgtaa atcaccctcc atggttgatt atttttcaac ctcaaaatgg tcatacacct
     2281 aacgcagccg cgattttcat cgcaactgca tgaattaaaa gaaaaaaaag gaatgtttcg
     2341 ttttcacgag taatacccag gtattaaaaa cccactgtaa ttacatgaaa ataaagaaat
     2401 aaaaggtaat accggcgtat taccacgtac gcgggcaagc gaaaaagcta taacgcttcc
     2461 cggccgacag gcggcccaga atccacaggg gcatgcagga tctcttcagt aaccttcgcc
     2521 attaaatcaa aaaggtcata ttcgccggga agctgcgtat cagtcgaacc ccgttgttca
     2581 tcatattcag atggcatggg gtgatatcca gtagcagaga ggttgttagc gggtttcggg
     2641 gcgcagccct gaaccagtca cgtagcgcta gcggagtgta tactggctga ctatgttggc
     2701 acggatgtgg aaatcagtga agtgcttcat gtagcaggag aaaaaaggcg gcaccagcgc
     2761 gtcagcagga tatgtagtac aggatatatt ccgcttcctc gctcactgac tcgctgcgct
     2821 cggtcgttcg gctgcggcga gcggtgccgg cttacaggcg gggcggaaat ttcctgggag
     2881 ataccaggaa ggagcttaac agggaagaga taaggccgcg gcgaagccgt ttttccatag
     2941 gctccgcccc cctgacaaac atcatgaaat ctgacgctca aatcagtggt ggcgaaaccc
     3001 gatgcggctt aaagatacca ggcgtttccc cctggtggct ccctcgtgct cccccgcatt
     3061 cctgcctttc ggtttaccgg tgtcattccg ctgttatggc cgcgtttatc tcattccacg
     3121 cctgacactc agttccgcga aggcagttcg ctcctggatg gactgtatgc acgaaccccc
     3181 cgttcagtcc g
//
