LOCUS       NZ_CP069689             3371 bp    DNA     circular CON 02-DEC-2023
DEFINITION  Escherichia coli O176:H45 strain MIN9 plasmid pMUB-MIN9-6, complete
            sequence.
ACCESSION   NZ_CP069689
VERSION     NZ_CP069689.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN17831482
            Assembly: GCF_017570665.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli O176:H45
  ORGANISM  Escherichia coli O176:H45
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3371)
  AUTHORS   Majewski,P.
  TITLE     Plasmid mediated mcr-1.1 colistin-resistance in clinical
            extraintestinal Escherichia coli strains isolated in Poland
  JOURNAL   Front Microbiol (2021) In press
REFERENCE   2  (bases 1 to 3371)
  AUTHORS   Majewski,P.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2021) Department of Microbiological Diagnostics
            and Infectious Immunology, Medical University of Bialystok,
            Waszyngtona 15A, Bialystok 15-269, Poland
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: Unicycler hybrid assembly v. v0.4.8-beta
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100x
            Sequencing Technology :: Illumina MiSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 12/02/2023 11:40:59
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,984
            CDSs (total)                      :: 4,862
            Genes (coding)                    :: 4,587
            CDSs (with protein)               :: 4,587
            Genes (RNA)                       :: 122
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 15
            Pseudo Genes (total)              :: 275
            CDSs (without protein)            :: 275
            Pseudo Genes (ambiguous residues) :: 0 of 275
            Pseudo Genes (frameshifted)       :: 81 of 275
            Pseudo Genes (incomplete)         :: 185 of 275
            Pseudo Genes (internal stop)      :: 48 of 275
            Pseudo Genes (multiple problems)  :: 37 of 275
            Pseudo Genes (short protein)      :: 3 of 275
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP069689.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3371
                     /organism="Escherichia coli O176:H45"
                     /mol_type="genomic DNA"
                     /strain="MIN9"
                     /serotype="O176:H45"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:2810408"
                     /plasmid="pMUB-MIN9-6"
                     /geo_loc_name="Poland: Bialystok"
                     /lat_lon="53.12523570 N 23.15905989 E"
                     /collection_date="2018-03-05"
                     /genotype="ST-6856"
     gene            complement(<104..571)
                     /locus_tag="JSU11_RS24510"
                     /old_locus_tag="JSU11_24515"
                     /pseudo=""
     CDS             complement(<104..571)
                     /locus_tag="JSU11_RS24510"
                     /old_locus_tag="JSU11_24515"
                     /inference="COORDINATES: protein motif:HMM:NF015397.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(561..884)
                     /locus_tag="JSU11_RS24515"
                     /old_locus_tag="JSU11_24520"
     CDS             complement(561..884)
                     /locus_tag="JSU11_RS24515"
                     /old_locus_tag="JSU11_24520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_024132211.1"
                     /translation="MLTIRVTDEEHARLLERCEGKRLAEWMRRVCLGEPVARTGKLPTL
                     SPPLLRHLAAIGNNLNQTARKVNSGQWSSIDRVHVVAALMAIEGELRQLRQAVREQGVR
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            948..1139
                     /locus_tag="JSU11_RS24520"
                     /old_locus_tag="JSU11_24525"
     CDS             948..1139
                     /locus_tag="JSU11_RS24520"
                     /old_locus_tag="JSU11_24525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165979.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNDLDADEQADICESLHDHA
                     DELYRSCLARFGDDGESN"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(2454..2687)
                     /locus_tag="JSU11_RS24525"
                     /old_locus_tag="JSU11_24530"
     CDS             complement(2454..2687)
                     /locus_tag="JSU11_RS24525"
                     /old_locus_tag="JSU11_24530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137389.1"
                     /translation="MTELEGHLLNALEHLQQDYMRRLNEWESAFAELQKMHEVTQRNNA
                     ILNERVVILSQQVQRLAGQVDRLSRLFIANNS"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(2694..3254)
                     /locus_tag="JSU11_RS24530"
                     /old_locus_tag="JSU11_24535"
     CDS             complement(2694..3254)
                     /locus_tag="JSU11_RS24530"
                     /old_locus_tag="JSU11_24535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_000067487.1"
                     /translation="MSNLLQMGTDFEKKLKERAASTENMLNSEFRKLEESVDKALSLNR
                     QKIRDAISEHTTSVKKQLDTLSTTVSMQFSTTEAELSRQQKKLLWRVIKGRILFPALTA
                     LSVTGGIFLGCWGLMEWQESKIAKNILTIREQENTLAKLEAKTWGVTFVNGENGKFLVL
                     PDGVKGENTWTVGDKNAVRLVRE"
                     /name="MbeB family mobilization protein"
     Typing          complement(1221..1301)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="87.805"
                     /coverage="93.0"
                     /evalue="8.68e-18"
     Typing          complement(3..571)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="98.421"
                     /coverage="99.0"
                     /evalue="8.550000000000001e-123"
     Typing          complement(2372..3369)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="89.645"
                     /coverage="99.0"
                     /evalue="2.03e-174"
ORIGIN
        1 gacgcgtttt tattgctccg gcatggtaaa gagcctcaag accccgtgta atcgcctctg
       61 cggcctcctg tttcgctttt ggcagattat ttggcgtgac aagcgtccgt ctgttctccg
      121 gtgcgttcgg gtcacgcagc ccgtaatgat gattaaccag cgtctgccag gcatttatcc
      181 tcggcctgtc cgcgcggtcg tagtacggct ggaggcgctt accgctctgt agctccatgt
      241 tcgggatgac aaaattcagt tcgagccgcc ccttatcctg gtgttccacc cagagaatgc
      301 tgtactggtc tttttcgaga ccgggcataa gaacacgctc aaagctcgtc atcactcttt
      361 cgcgttcacc cggcggtaac tccttctccg cgaacgacag aacgcctgaa gtgtatttct
      421 tcgagaatgg cgtggaatca atgagttccc gcacttcttc cgggttaccc cggagcaccg
      481 ttgcgccttc gcggttacgc tcccgcccca gcaggtaatc gaccgggcca cttccaccgc
      541 ctttccccct ggcatgaaat ttaactatca tcccgtaccc cctgttccct gacagcctgc
      601 cgcaactggc gcaattcacc ctcgatggcc atcagtgcgg ccaccacatg aacccggtca
      661 atcgaagacc actggccgct gttcaccttc cgggctgtct ggttcaggtt gttcccgatg
      721 gcagccagat gccgcagcaa tggcggcgat agtgtcggca gttttcctgt gcgtgcaacc
      781 ggctccccca gacagacccg ccgcatccat tccgccagtc gtttaccctc gcagcgttcc
      841 agtaaccggg cgtgctcctc atcggtaacc cgtatcgtga gcatcttctc gcgtttcatc
      901 ggtatcattc cccctaacaa atcccccata caaggagcct taagaccatg accaaacagg
      961 aaaaaacagc ccttaacatg gctcgcttta tcagaagcca gacgctaacc ctgctggaaa
     1021 aactcaacga tctggacgcg gacgaacagg cagacatctg cgaatcactt cacgaccacg
     1081 ccgacgaact ctaccgcagt tgcctcgcac gcttcggcga tgacggtgaa agcaactgaa
     1141 acatgcagct cccggagacg ttcacagctt gcctgtgagc ggatgccggg agcagacaag
     1201 cccgccaggg cgcgtcagtg cgggtgtcgg ggcgcagccc tgacccagtt cacagagcga
     1261 tagcgatgtg tatactggct tatctcattt tgcatcggtg cagaatggat gggaagtgca
     1321 ccatgtcagg agtaaaaagc cgcacagatg taaaaacagc gcacccgata aacagcacac
     1381 acgcggacac tggcacaggc gcgtaagtgc acaatgcttg catctgtgac tcttccgctt
     1441 cctcgcccac tcgctcacta cgttcggtcg ctggggctgc ggcgagcggt gggatcactc
     1501 tcgttgtgat gcagatccgt tttttttttt gcactggagt tatcaggagt tgcggtgcac
     1561 atgggatcgt gcgatatgcc ggatggtgaa aggttggagg gaacggcaac aactcacccc
     1621 tcccagtgaa gggacgggta gtttgccgct ctcgatatgc cggacggagc caaggccatc
     1681 gatcacgggg cgcgtcagtg gaagaacccg cgcctgcgtg acgtatgcgt cttcgccgca
     1741 ccatctcacc cgtcacgcca ccacataccg ggaactgccg ccaaggtcta actcaaccaa
     1801 tctagccgcg ctacatacgg tttttgtggc aactcatcag gtgtccgctg tcgatgaaac
     1861 tctctgcgcc ggtagcggaa ctctcacgcg tgcggtcgcc gtcgtcacag ctcccccaac
     1921 cgcaaacgat tctcgcaaat acgcaagtat ctgcaaaaac aggttttttt cggggttcct
     1981 gggagactgt gctgttgaaa aattcttgaa gaatacagaa ggctcatgca aactgaatga
     2041 actggtttct gttgtggatc tcaacagttt ccacaggaac attgaggagg tcagagacct
     2101 caatgaaccc gaaacagctc gccttcctgg cgagctgttt tcgttctacg cccgccgacg
     2161 aaagatcgca agtgcaataa cgagattttt cacgtcttta cgctcaaaaa gtaaaaaaac
     2221 catgttttcc cctggctgaa tccccaaaaa cgcgtgaaat gcgttttcag gatgttgcat
     2281 agctatgcac aaatacggca cacgatttgt ttcgctcgcc ccagccgtcg aactggtcaa
     2341 aatggcaaga tttcgtcact tccccggtca catctccatt gatggcccac tccgaacccg
     2401 tgccgcgcgt tcctgtacct catgtctgta ccgggcacgc tcctgttcat cccttagctg
     2461 ttgttcgcga taaataaccg actcaagcga tccacttgcc cggctaatct ctgcacctgc
     2521 tgactcaata tcacgacgcg ctcgttcagt atcgcgttgt tccgttgcgt aacctcgtgc
     2581 atcttctgca attccgcgaa ggcgctctcc cactcgttca gccgccgcat atagtcctgt
     2641 tgcaaatgct ctaaggcgtt cagcaaatgc ccttccagtt ccgtcatact cgtttactcc
     2701 ctgaccagcc tcacggcgtt cttgtcgccc accgtccagg tgttttcccc tttcaccccg
     2761 tccggcaaca ccagaaattt cccgttctcg ccattgacga acgtcacccc ccaggttttt
     2821 gcctcgagtt tcgccagcgt gttttcctgc tctctgatag tcaggatatt tttcgcaatc
     2881 ttgctttcct gccactccat cagcccccag caccccagga aaatgccgcc cgtgacgctg
     2941 agcgcggtca gcgccggaaa cagtatccgc cctttgatca cccgccagag cagttttttc
     3001 tgctgccggg acagttcggc ctctgtggtg ctgaactgca tgctcacggt ggtgctcagc
     3061 gtatccagtt gctttttcac ggatgttgta tgttcgctga tagcgtctct gattttctgc
     3121 ctgtttaagc tcaatgcctt gtctacagac tcttcgagct tcctgaactc gctgttcagc
     3181 atgttctcgg tagaagcggc tcgctctttc agtttcttct cgaagtctgt tcccatttgt
     3241 aaaagattgc tcatacagcg ccccctttag cctgagattc cgccccccct ccgggtcggc
     3301 aatactgatg ctgcttttgg ttgtcctcac cacctcaaaa cctgccgctg taagcgcctc
     3361 tgtgacatcc t
//
