LOCUS       NZ_CP069688             4018 bp    DNA     circular CON 02-DEC-2023
DEFINITION  Escherichia coli O176:H45 strain MIN9 plasmid pMUB-MIN9-5, complete
            sequence.
ACCESSION   NZ_CP069688
VERSION     NZ_CP069688.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN17831482
            Assembly: GCF_017570665.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli O176:H45
  ORGANISM  Escherichia coli O176:H45
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4018)
  AUTHORS   Majewski,P.
  TITLE     Plasmid mediated mcr-1.1 colistin-resistance in clinical
            extraintestinal Escherichia coli strains isolated in Poland
  JOURNAL   Front Microbiol (2021) In press
REFERENCE   2  (bases 1 to 4018)
  AUTHORS   Majewski,P.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2021) Department of Microbiological Diagnostics
            and Infectious Immunology, Medical University of Bialystok,
            Waszyngtona 15A, Bialystok 15-269, Poland
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: Unicycler hybrid assembly v. v0.4.8-beta
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100x
            Sequencing Technology :: Illumina MiSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 12/02/2023 11:40:59
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,984
            CDSs (total)                      :: 4,862
            Genes (coding)                    :: 4,587
            CDSs (with protein)               :: 4,587
            Genes (RNA)                       :: 122
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 15
            Pseudo Genes (total)              :: 275
            CDSs (without protein)            :: 275
            Pseudo Genes (ambiguous residues) :: 0 of 275
            Pseudo Genes (frameshifted)       :: 81 of 275
            Pseudo Genes (incomplete)         :: 185 of 275
            Pseudo Genes (internal stop)      :: 48 of 275
            Pseudo Genes (multiple problems)  :: 37 of 275
            Pseudo Genes (short protein)      :: 3 of 275
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP069688.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4018
                     /organism="Escherichia coli O176:H45"
                     /mol_type="genomic DNA"
                     /strain="MIN9"
                     /serotype="O176:H45"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:2810408"
                     /plasmid="pMUB-MIN9-5"
                     /geo_loc_name="Poland: Bialystok"
                     /lat_lon="53.12523570 N 23.15905989 E"
                     /collection_date="2018-03-05"
                     /genotype="ST-6856"
     gene            join(3767..4018,1..276)
                     /locus_tag="JSU11_RS24475"
                     /old_locus_tag="JSU11_24480"
     CDS             join(3767..4018,1..276)
                     /locus_tag="JSU11_RS24475"
                     /old_locus_tag="JSU11_24480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004883515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_016246513.1"
                     /translation="MKCFWACSVSVVLAMSFTVAAAENNDNVTYTKPSAGRDLYLTCKG
                     YQVKGASVINVEKPSYLEGTGEQVEFAIITPVGASDGLKVFFDSWGFSSFGLIESHVMH
                     EHWSLVHVGGRASVKGEKIDYSFVDNQPQEGGSYKPKTGVFLYGCMNSDKRPSWVEKIY
                     TRTGVFRGGIED"
                     /name="hypothetical protein"
     gene            531..743
                     /locus_tag="JSU11_RS24480"
                     /old_locus_tag="JSU11_24485"
     CDS             531..743
                     /locus_tag="JSU11_RS24480"
                     /old_locus_tag="JSU11_24485"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_080111754.1"
                     /translation="MGDFERTFGAGADFDRIIDENNSEYFREDRRKTKNSKKNFSSFQD
                     ALVWAKANPGKTIIRSPDGVGFIEK"
                     /name="hypothetical protein"
     gene            complement(1124..1285)
                     /locus_tag="JSU11_RS24485"
                     /old_locus_tag="JSU11_24490"
     CDS             complement(1124..1285)
                     /locus_tag="JSU11_RS24485"
                     /old_locus_tag="JSU11_24490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_208185727.1"
                     /translation="MHEPPVQSDRCALSGNYRLESNPERHDKTPLAAATGNWFRGVSLE
                     VMRRLRLN"
                     /name="replication initiation protein"
     gene            1933..2256
                     /locus_tag="JSU11_RS24490"
                     /old_locus_tag="JSU11_24495"
     CDS             1933..2256
                     /locus_tag="JSU11_RS24490"
                     /old_locus_tag="JSU11_24495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_024191642.1"
                     /translation="MLTMWVTEDEHRRLLERCEGKQLAAWMRQICLDEKPSRAGKLPSI
                     SPALLRQLAGMGNNLNQIARQVNAGGGTGHDRVQVVAVLMAIDAGLERLRHAVLEKGAD
                     DDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            2465..>2899
                     /locus_tag="JSU11_RS25090"
                     /pseudo=""
     CDS             2465..>2899
                     /locus_tag="JSU11_RS25090"
                     /inference="COORDINATES: protein motif:HMM:NF015397.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            2934..3419
                     /locus_tag="JSU11_RS24500"
                     /old_locus_tag="JSU11_24505"
     CDS             2934..3419
                     /locus_tag="JSU11_RS24500"
                     /old_locus_tag="JSU11_24505"
                     /inference="COORDINATES: protein motif:HMM:NF016710.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_061390563.1"
                     /translation="MNSLLTLAKDLEQKSKAQQQTTGEMLKAAFSEHEKSVRAELSESE
                     KRISAAILDHDRKLSSAMSQRTKGMLRMISQTWLTIVLVSALLIASSAGILWWQGQQIL
                     DNYTTIQEQKSTQAMLSEKNNGVQLTTCGEERRRCVRVNPEAGRFGEDSSWMILAGK"
                     /name="MbeB family mobilization protein"
     gene            3423..3638
                     /locus_tag="JSU11_RS24505"
                     /old_locus_tag="JSU11_24510"
     CDS             3423..3638
                     /locus_tag="JSU11_RS24505"
                     /old_locus_tag="JSU11_24510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_072048928.1"
                     /translation="MTELEKQLLSALEQLQQDYSKRLDEWENAFAEWRTMSGLMQRENA
                     ALSERVTGLSRQVQSLSDQLRRLSQG"
                     /name="MbeD family mobilization/exclusion protein"
     Typing          1643..1820
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="87.222"
                     /coverage="83.0"
                     /evalue="4.5e-50"
     Typing          complement(1174..1693)
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="89.734"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          2247..3730
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="91.717"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 gtcttttttg attcatgggg tttttcttca tttggattga ttgaaagcca tgtgatgcat
       61 gagcactggt ctttagttca tgttgggggg cgtgcttcag tcaaaggtga aaagattgat
      121 tattcatttg tagataacca gccacaggag ggggggagct ataaacccaa aacaggtgtg
      181 ttcctgtacg gctgcatgaa ctcggataaa agaccatctt gggtcgaaaa gatctacaca
      241 agaacaggtg tgtttcgagg gggcatagaa gactaaaata ctgcttacag catgaaaatc
      301 cattgaacag agtgtcagtt catttccgtg cataactatg cataccgtaa gaacttacgg
      361 ggggcattta tcggcgtttt tacgtggtaa gaggtgctct tttgcggttc atggcgcaga
      421 acggtgctga ggctgtctgc ggcggtgcga acaagggatg ttatggtaaa taagagtgtc
      481 ttttggatat tttaaatctg tttttttatt aaatttaatg ggggcagaaa atgggggatt
      541 ttgagcgtac ctttggagct ggtgctgatt ttgatcggat aattgatgag aataacagcg
      601 aatattttcg agaagatcgc cgaaaaacta aaaattcaaa aaaaaatttc tcttcttttc
      661 aagatgcttt ggtttgggcg aaagctaatc ctggcaaaac gataataaga tctccagacg
      721 gtgtggggtt tattgaaaaa tgaaagagtg gattcactgt aaaacaacag tcattttttg
      781 gacaacactg tctatatacg tactcatatg tttcacagtg tcgtatgttg gagtctatgt
      841 tacgtacgtt gcagggccta ttttgttagt tacaggcatt cttgcatggc ttacgaaacc
      901 tagctgtaat ccaaagtgac tgggctagac gaactttatc cccccataaa acccgctttt
      961 ttaaacggat cttcttgaga tcattcatgg agaatcgtaa tctcttagca ctgtaaacga
     1021 aaaaaccgcc cagcaaggcg gttttttcga aggttctcag agcagcaact ctttgaaccg
     1081 aggtaactgg cttggaggag cacagtcacc aaaacttgtc ctttcagttt agccttaacc
     1141 ggcgcataac ttcaagacta actcctctaa accagttacc agtggctgct gccagtggcg
     1201 ttttgtcgtg tctttccggg ttggactcaa gtcgatagtt accggataag gcgcagcggt
     1261 cggactgaac ggggggttcg tgcatacagt ccagcttgga gcgaactgcc tacccggaac
     1321 tgagtgtcag gcgtggaatg agataaacgc ggccataaca gcggaatgac accggtaaac
     1381 cgaaaggcag gaacaggaga gcgcacgagg gagctaccag ggggaaacgc ctgggatctt
     1441 tatagtcctg tcgggtttcg ccaccactga tttgagcgtc agatttcgtg atgcttgtca
     1501 ggggggcgga gcctatggaa aaacggcaac gccgcatcct ttttccgccc tttctgattg
     1561 atttccgctt agtctcttta tccagccatt cgtaagctgg caacgcccgc cgcagtctca
     1621 cggcaggagc gcagcgaccg agtgagcgag gaggcgtcat ttcaccggga tctgcatttc
     1681 gcacttacgc cccacagcag cattttctcc ctgccacatg aagcactttc ctgaatcccc
     1741 actccgattt accatgttaa gccagtatac actccgctag cgctacgtga ctggttcagg
     1801 gctgcgcccc gaaacccgct gaaaagtact gccgcgccct gcgggcttgc ccaacaccgc
     1861 tccggacagg aaaaaatttc ccgcccgaac cggtgttatc cggaggatgg tttggcagaa
     1921 aagcgcaaca aaatgctcac tatgtgggtg acagaagacg agcaccgccg acttctggag
     1981 cgctgtgagg gtaaacaact cgcggcctgg atgcgccaga tatgcctgga cgaaaagccc
     2041 tcgcgtgctg gcaaacttcc atccatctcg ccggcgctgc ttcgtcagct tgccggcatg
     2101 ggtaacaacc tcaaccagat tgcccggcag gttaacgccg gtggcggtac tgggcacgat
     2161 cgcgtgcagg ttgtcgccgt gctgatggcc attgatgccg gactcgagcg gctgcggcac
     2221 gccgtgctgg aaaagggggc agacgatgat cgttaagttt catcccagag gccgcggcgg
     2281 tggcggcggt ccggtggatt atctgctggg gaaagaccgg cagcgcgacg gcgccagcgt
     2341 tctgcagggt aagccggagg aagtgcggga gcttattgac gcctcgcctt acgccaagaa
     2401 atacacctcc ggcgtgctgt cttttgccga acaggatttg ccgcccggcc agcgtgaaaa
     2461 gctgatggcg agctttgagc gggttctgat gcccggactc gataaagacc agtacagcgt
     2521 actgtgggtt gagcaccggg acaaagggcg gctggagctg aacttcctga tcccgaacac
     2581 ggagctgctg accggcagac gtctccagcc gtattacgac cgcgccgacc gtccgcgcat
     2641 tgatgcctgg cagaccatag tgaacggcag actggggctg cacgacccga acgcgccgga
     2701 aaaccggcgg gcgctggtta caccgtccgc gctgccggaa gcgaagcagg aagccgccca
     2761 ggcgattacg cgcggcctac tttcccttgc ctcatccggg gagctgaaaa cccgtcagga
     2821 cgtcactgag gcgctggaaa gtgcgggttt tgaggtggtg cgcaccacga aaagcagcat
     2881 cagcattgcc gaccctgacg gggggcgaaa catccgactg aagggagcca tctatgaaca
     2941 gtcttttaac gctggcgaag gacttagagc agaaatcgaa agcgcagcag cagactaccg
     3001 gcgagatgct gaaagccgca ttcagcgagc acgagaagtc tgtcagagcg gaactgagcg
     3061 aaagcgagaa gagaatcagc gccgccatcc tcgaccacga ccggaagctg tcctcagcca
     3121 tgagccaacg cacgaaaggg atgctgcgta tgatcagcca gacgtggctg accatagtcc
     3181 tggtctccgc gctactgatc gcctcgagcg cgggcattct gtggtggcag gggcaacaga
     3241 tactcgacaa ctacacgacc atccaggaac agaagagcac gcaggccatg ctttcagaga
     3301 agaacaacgg cgtccagctc acgacctgcg gggaggagag acgccgctgc gtgagggtga
     3361 acccggaagc gggaagattc ggagaggatt cgagctggat gatactggcg gggaaatagc
     3421 acatgacgga actggaaaaa cagttgctga gcgcattaga gcagctacag caggactact
     3481 cgaaaaggct ggacgagtgg gagaacgcct tcgcggaatg gcggacgatg tctgggctta
     3541 tgcaacggga gaacgcggcg ctgagcgagc gcgtcacggg cttgagcagg caggtgcaga
     3601 gtttgagcga tcagctgcgc cgattgtcgc aaggctgaat ggcatcgagg cgcaccggga
     3661 gcaggagcgc gcggcgcagc accagaaagc gctggagctg gaacgatcgc agcggcagaa
     3721 aggttttgag cttggaatgt agttaatcaa tgaaaaggga tgcgttatga aatgtttctg
     3781 ggcttgctca gtctcggtgg ttttggctat gtcatttaca gtcgctgctg cggagaataa
     3841 cgacaacgtt acatatacaa aaccgtcagc cgggagggac ttatatctta cctgtaaagg
     3901 ttatcaggtg aagggggcat ctgtaattaa cgtcgagaaa ccttcttacc ttgaaggtac
     3961 tggtgagcag gttgaatttg caatcattac tcctgttggt gcgtctgacg gactcaag
//
