LOCUS       NZ_CP069687             5309 bp    DNA     circular CON 02-DEC-2023
DEFINITION  Escherichia coli O176:H45 strain MIN9 plasmid pMUB-MIN9-4, complete
            sequence.
ACCESSION   NZ_CP069687
VERSION     NZ_CP069687.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN17831482
            Assembly: GCF_017570665.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli O176:H45
  ORGANISM  Escherichia coli O176:H45
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5309)
  AUTHORS   Majewski,P.
  TITLE     Plasmid mediated mcr-1.1 colistin-resistance in clinical
            extraintestinal Escherichia coli strains isolated in Poland
  JOURNAL   Front Microbiol (2021) In press
REFERENCE   2  (bases 1 to 5309)
  AUTHORS   Majewski,P.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2021) Department of Microbiological Diagnostics
            and Infectious Immunology, Medical University of Bialystok,
            Waszyngtona 15A, Bialystok 15-269, Poland
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: Unicycler hybrid assembly v. v0.4.8-beta
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100x
            Sequencing Technology :: Illumina MiSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 12/02/2023 11:40:59
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,984
            CDSs (total)                      :: 4,862
            Genes (coding)                    :: 4,587
            CDSs (with protein)               :: 4,587
            Genes (RNA)                       :: 122
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 15
            Pseudo Genes (total)              :: 275
            CDSs (without protein)            :: 275
            Pseudo Genes (ambiguous residues) :: 0 of 275
            Pseudo Genes (frameshifted)       :: 81 of 275
            Pseudo Genes (incomplete)         :: 185 of 275
            Pseudo Genes (internal stop)      :: 48 of 275
            Pseudo Genes (multiple problems)  :: 37 of 275
            Pseudo Genes (short protein)      :: 3 of 275
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP069687.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5309
                     /organism="Escherichia coli O176:H45"
                     /mol_type="genomic DNA"
                     /strain="MIN9"
                     /serotype="O176:H45"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:2810408"
                     /plasmid="pMUB-MIN9-4"
                     /geo_loc_name="Poland: Bialystok"
                     /lat_lon="53.12523570 N 23.15905989 E"
                     /collection_date="2018-03-05"
                     /genotype="ST-6856"
     gene            complement(join(5180..5309,1..62))
                     /locus_tag="JSU11_RS24430"
                     /old_locus_tag="JSU11_24435"
     CDS             complement(join(5180..5309,1..62))
                     /locus_tag="JSU11_RS24430"
                     /old_locus_tag="JSU11_24435"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165975.1"
                     /translation="MTKQEKTALNMARFIRGQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENR"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            94..449
                     /locus_tag="JSU11_RS24435"
                     /old_locus_tag="JSU11_24440"
                     /pseudo=""
     CDS             94..449
                     /locus_tag="JSU11_RS24435"
                     /old_locus_tag="JSU11_24440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001308260.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            658..>1089
                     /locus_tag="JSU11_RS25085"
                     /pseudo=""
     CDS             658..>1089
                     /locus_tag="JSU11_RS25085"
                     /inference="COORDINATES: protein motif:HMM:NF015397.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            1127..1657
                     /locus_tag="JSU11_RS24445"
                     /old_locus_tag="JSU11_24450"
     CDS             1127..1657
                     /locus_tag="JSU11_RS24445"
                     /old_locus_tag="JSU11_24450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001673158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_001673158.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTEKMLNDEFRKLEESVNRELTSNE
                     SLIRNAINDHTTALKELLERYQKTTVDTMDAHWKTVLKMSVKRWLWLIIVSVLMFATTG
                     SLLWYQGMKINANMNILREQKESLEKLNAKTWGVRYHEDSNGRFLVLPKGMKAETNWTK
                     DNGKLNAVRLVQE"
                     /name="MbeB family mobilization protein"
     gene            1664..1897
                     /locus_tag="JSU11_RS24450"
                     /old_locus_tag="JSU11_24455"
     CDS             1664..1897
                     /locus_tag="JSU11_RS24450"
                     /old_locus_tag="JSU11_24455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137437.1"
                     /translation="MTELERHLLNALEQLQQDYMQRLNEWENAFVELQKMFSLTQRDNA
                     MLNGRVMQLSQQVQHLSERTERLSQLYSENRR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            <2657..>2752
                     /locus_tag="JSU11_RS24460"
                     /old_locus_tag="JSU11_24465"
                     /pseudo=""
     CDS             <2657..>2752
                     /locus_tag="JSU11_RS24460"
                     /old_locus_tag="JSU11_24465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013356662.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /name="amino acid ABC transporter substrate-binding
                     protein"
     gene            2898..3713
                     /locus_tag="JSU11_RS24465"
                     /old_locus_tag="JSU11_24470"
     CDS             2898..3713
                     /locus_tag="JSU11_RS24465"
                     /old_locus_tag="JSU11_24470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032492145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(3')-Ia"
                     /protein_id="WP_000018330.1"
                     /translation="MSHIQRETSCSRQRLNSNLDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAAFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
                     /name="aminoglycoside O-phosphotransferase APH(3')-Ia"
     gene            complement(<3723..>3995)
                     /locus_tag="JSU11_RS24470"
                     /old_locus_tag="JSU11_24475"
                     /pseudo=""
     CDS             complement(<3723..>3995)
                     /locus_tag="JSU11_RS24470"
                     /old_locus_tag="JSU11_24475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021138877.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /name="hypothetical protein"
     ARG             2899..3710
                     /name="aph(3')-Ia"
                     /gene="aph(3')-Ia"
                     /gene_name="aminoglycoside O-phosphotransferase APH(3')-Ia"
                     /tool="amrfinderplus"
                     /drug_class="AMINOGLYCOSIDE"
                     /identity="100.0"
                     /coverage="100.0"
     Typing          5005..5086
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="1.3400000000000002e-35"
     Typing          4361..4548
                     /name="rep_cluster_2401"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="4.63e-94"
     Typing          95..219
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="1.31e-53"
     Typing          440..2001
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 gttaatgtct ggcctctgat gaagcgggcc atgttaaggg cggttttttc ctgtttggtc
       61 actgatgcct ccgtgtaagg gggggttctg ttcatggggg taatgatacc gatgaaacgc
      121 gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgctgt
      181 gagggtaaac aactggcggt atggatgcgg cgggtctgct gggtgagccg gttgcccgtt
      241 ccgggaagct gccgacactg gcaccgccgt tactgcgtca gctggccgcc atcgggaata
      301 accttaacca gacagcgagg aaggtgaaca gcgggcagtg gtcttccggt gaccgggttc
      361 aggtggtggc cgcactgatg gccatcgggg atgagctgcg ccggctgcgt ctggcggtca
      421 gggaacaggg ggcgcgggat gatagttaag tttcatgcca ggggaaaagg tggtggcagt
      481 ggtccggttg attacctgct gggaagggag cgtaaccggg aaggggcaac ggtgcttcag
      541 gggaatccgg aagaggtccg ggaactcatc gatgccacgc catttgcgaa gaaatacacg
      601 tcaggtgttc tgtcgttcgc ggagaaggag ctgccgcctg gagggcgtga aaaagtgatg
      661 gcgagctttg agcgtgtact gatgcccggt ctcgagaaaa atcagtacag catcctgtgg
      721 gtggagcacc aggacaaggg acggcttgag ctgaattttg tcattccgaa catggagcta
      781 cagaccggaa aacgcctcca gccgtactac gaccgggcag acaggccgag aattgatgcc
      841 tggcagacgc tggtgaatca ccattacggt ctgcatgacc ccaacgcccc ggagaaccgc
      901 cggacgctga cccttcctga taacctgccg gaaacgaaac aggcgcttgc tgagggcgtc
      961 acacgcggga tagacgcgct ttaccatgcc ggagagataa aaggccgtca ggatgtgatt
     1021 caggcgctca cagaggcggg gcttgaagtg gtcagggtga cgcgaagcag tatcagcatt
     1081 gcagacccga acggcgggag gaatatcaga ctgaaaggag cgttttatga gcaatctttt
     1141 acagacgggc gcggagttcg agaaaaagct gaaagagaga gccgaatcta cagagaaaat
     1201 gctgaacgac gagttcagga agctcgaaga atctgtaaac agggaactga cctcaaacga
     1261 atctttaatc aggaacgcta tcaacgacca tacaacagcc ctgaaagaac tgctggagag
     1321 gtatcagaaa acgaccgtcg atacgatgga cgcgcactgg aaaaccgtac tgaaaatgag
     1381 cgtgaaacgc tggctgtggc tgattatcgt ctcagtactg atgtttgcca cgaccgggag
     1441 tctgctgtgg taccagggca tgaagataaa cgcgaatatg aatattctcc gcgagcagaa
     1501 agagagtctg gagaagctga acgcgaagac atggggcgtg cgttatcacg aggacagcaa
     1561 cggacgtttt ctggtgttgc cgaaggggat gaaagccgaa acgaactgga cgaaggacaa
     1621 cggcaaactg aacgcagtga gactggtgca ggagtgacag agcatgacgg agctggaaag
     1681 acatttgctg aacgcattag agcaactgca acaggactat atgcagcggc tgaacgaatg
     1741 ggagaacgcc ttcgtggaat tgcagaaaat gttttcgctt acgcaacggg acaacgcgat
     1801 gctgaacggg cgagtcatgc agttgagtca gcaggtgcag cacttgagcg agcggacaga
     1861 acgcttgagc cagttataca gcgagaacag gagataaggg atgaacggct gatgcaggaa
     1921 cgcgaacatg agttatctct ggagcgggag cgtcagccgg aaatccagga gcggacactg
     1981 gatggccctt cgctgggatg gtgaaaccat gaaaaatgac gtgttcagtg gattaaggcg
     2041 ggctaatgtg gcctgtagca tctggttgca taggtattca tgcggttaaa atttatcagg
     2101 tgcgatcgcg gcagtttttc gggtggtttg ttgccatttt tacctgtctg ctgccgtgat
     2161 cgcaccgaac gcgttttggc ggtgcgtaca atgcgacgtt atggtaaatg gagcggattt
     2221 tcaggaagga gtgatactaa caactcgaac actgtgcaaa taatgatttt tttaaaatat
     2281 cggtaacgat aataaattat ttcgttttac gccggtaata aatataatgt agtttcagta
     2341 tataaaaaaa tatatgaggt gtcttgtggg agctttcatg aatcttcaac aacgtataaa
     2401 taaactacca caattatcat ccagtttttc atttggaaaa gatattgata atattcattc
     2461 atttatcttt aacgaaactt ctaaagataa aattgaagat ttactaagaa aatgggtgtc
     2521 aggcaatcag ccctgtgtat ttggtaaatt ggcaagcaag aaaattaaag ggcttgattt
     2581 ccatttatct attgtaaata gcccccaact atataatgat gatggtcatt tgtttgattt
     2641 tttgcgtaat gaaagagttc atgccatgtt cctcgactac gaagcggcca aagattatgc
     2701 gacccgctcc aggctggtca cggcggcgaa tatcccttct ttcggagcgc cagaatgatg
     2761 ttggtctggc cggattttca tatggcggga accacgttgt gtctcaaaat ctctgatgtt
     2821 acattgcaca agataaaaat atatcatcat gaacaataaa actgtctgct tacataaaca
     2881 gtaatacaag gggtgttatg agccatattc aacgggaaac gtcttgctcg aggcagcgat
     2941 taaattccaa cctggatgct gatttatatg ggtataaatg ggctcgcgat aatgtcgggc
     3001 aatcaggtgc gacaatctat cgattgtatg ggaagcccga tgcgccagag ttgtttctga
     3061 aacatggcaa aggtagcgtt gccaatgatg ttacagatga gatggtcaga ctaaactggc
     3121 tgacggaatt tatgcctctt ccgaccatca agcattttat ccgtactcct gatgatgcat
     3181 ggttactcac cactgcgatc cccgggaaaa cagcattcca ggtattagaa gaatatcctg
     3241 attcaggtga aaatattgtt gatgcgctgg cagcgttcct gcgccggttg cattcgattc
     3301 ctgtttgtaa ttgtcctttt aacagcgatc gcgtatttcg tctcgctcag gcgcaatcac
     3361 gaatgaataa cggtttggtt gatgcgagtg attttgatga tgagcgtaat ggctggcctg
     3421 ttgaacaagt ctggaaagaa atgcataagc ttttgccatt ctcaccggat tcagtcgtca
     3481 ctcatggtga tttctcactt gataatctta tttttgacga ggggaaatta ataggttgta
     3541 ttgatgttgg acgagtcgga atcgcagacc gataccagga tcttgccatt ctatggaact
     3601 gcctcggtga attttctcct tcattacaga aacggctttt tcaaaaatat ggtattgata
     3661 atcctgatat gaataaattg cagtttcatt tgatgctcga tgagtttttc tgatagttag
     3721 tctttaatga atgcggttgg cgcatcggcg ggcaccttgc gcgcgctgtc gctgttcatg
     3781 ccgcgcagca tgtcgatggc atcgagcaca cccttggcgg cgggcgcagc ccgcaatttg
     3841 agcacgccca ggaactgcgg cgcgtagcgg cgtagcgtgg catagctttc accgatgtgg
     3901 tgcaggaaat caaagtcggc aggccgcgcc aatgtttgcg cttcggtgac gctggcggcg
     3961 aaggtgtccc agggcataac ggcctcgagg attgcccccc agcctccatc gataccgtgc
     4021 caaatgcacg taaaaaacgg gagcgtttct ggtgtcggaa tgtaaccaag ggggtcagag
     4081 gcgcgctaaa acgctctcag gggagtcaaa tagagacgtt tgtaacaaaa agatcaaagg
     4141 atcctcttga gatccttttt ttctgcgcgt aatctgctgc ctgtaaacga aaaaaccgcc
     4201 ttgcagggcg gtttgatcga aggttaagtc agcttgcaga ctgcttaacc aggtaactgt
     4261 cttatgcgag acgagtcacc aaaattgttc ttctagtgta gccgcactcc ggcagccact
     4321 tcaagtactc tctaaatatc tcgcacatga cttttaccaa tggctgctgc cagtggcgct
     4381 ttgtggtgtc ttttccgggt tggacccaag agaacagtta ccggagaagg cgcagcagtc
     4441 gggctgaacg gggggttcct gcatacagcc cagcccggag cgaactgcct aagtcgcccc
     4501 gagataccag aagacgtgag ctatgagaaa gcgccacgtg tcccgtaagg gagaaaggcg
     4561 gaaaaggtat ccggtaatcg atatggcggg aacgtggggg cgcaggaggg agcgattcac
     4621 cggaaacggt gatgatcttt aagccacgtc gggtttcgcc accgctggcg gatttcatgg
     4681 ttgagccgca aggctcccac agatgcaccg aaaaagtgtc tgttttgtga agtccgtcag
     4741 gggggcggag cctatggaaa aagcgcctcc ggcgcggcct ttcccggggt ttcgtttttt
     4801 gcgcacatgt tctttcctgg tttatccccg ggttctgtgg ataacagggt tagcgttttt
     4861 gagaaaggag agtccgctcg ccgcagccga acgaccgagc gtagcgagtc agtgagcgag
     4921 gaagcggaag agcgcctgaa ctggcatttt ctccttacgc atctgtgcgg catttcacac
     4981 cggacatggc gcacttttca tacaatccgc actgatgccg aatggttaag ccagtataca
     5041 ctccgctatc gctacgtgac tgggtcaggg cttcgccccg acaccccaaa ggtcaacggc
     5101 ctgacgcgcc ctggcgggct tgtctgctcc cggcatccgc tcacagacaa gctgtgaccg
     5161 tctccgggag ctgcatgtgt cagcggtttt caccgtcatc cccgaagcgt gcgaggcaac
     5221 tgcggtaaag ctcatcggcg tggtcgtgaa gcgattcaca gatgtctgcc tgttcatccg
     5281 cgtccagttc gttgagcttc tccagcagc
//
