LOCUS       NZ_CP069686             5792 bp    DNA     circular CON 02-DEC-2023
DEFINITION  Escherichia coli O176:H45 strain MIN9 plasmid pMUB-MIN9-3, complete
            sequence.
ACCESSION   NZ_CP069686
VERSION     NZ_CP069686.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN17831482
            Assembly: GCF_017570665.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli O176:H45
  ORGANISM  Escherichia coli O176:H45
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5792)
  AUTHORS   Majewski,P.
  TITLE     Plasmid mediated mcr-1.1 colistin-resistance in clinical
            extraintestinal Escherichia coli strains isolated in Poland
  JOURNAL   Front Microbiol (2021) In press
REFERENCE   2  (bases 1 to 5792)
  AUTHORS   Majewski,P.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2021) Department of Microbiological Diagnostics
            and Infectious Immunology, Medical University of Bialystok,
            Waszyngtona 15A, Bialystok 15-269, Poland
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: Unicycler hybrid assembly v. v0.4.8-beta
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100x
            Sequencing Technology :: Illumina MiSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 12/02/2023 11:40:59
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,984
            CDSs (total)                      :: 4,862
            Genes (coding)                    :: 4,587
            CDSs (with protein)               :: 4,587
            Genes (RNA)                       :: 122
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 15
            Pseudo Genes (total)              :: 275
            CDSs (without protein)            :: 275
            Pseudo Genes (ambiguous residues) :: 0 of 275
            Pseudo Genes (frameshifted)       :: 81 of 275
            Pseudo Genes (incomplete)         :: 185 of 275
            Pseudo Genes (internal stop)      :: 48 of 275
            Pseudo Genes (multiple problems)  :: 37 of 275
            Pseudo Genes (short protein)      :: 3 of 275
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP069686.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5792
                     /organism="Escherichia coli O176:H45"
                     /mol_type="genomic DNA"
                     /strain="MIN9"
                     /serotype="O176:H45"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:2810408"
                     /plasmid="pMUB-MIN9-3"
                     /geo_loc_name="Poland: Bialystok"
                     /lat_lon="53.12523570 N 23.15905989 E"
                     /collection_date="2018-03-05"
                     /genotype="ST-6856"
     gene            349..909
                     /locus_tag="JSU11_RS24395"
                     /old_locus_tag="JSU11_24400"
     CDS             349..909
                     /locus_tag="JSU11_RS24395"
                     /old_locus_tag="JSU11_24400"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_001522453.1"
                     /translation="MSNLLQMGTDFEKKLKERAASTENMLNSEFRKLEESVDKALSLNR
                     QKIRDAISEHTTSVKQQLDTLSTTVSTQFSTTEAELSRQQKKLLWQVIKGRILFPALTA
                     LSVTGGIFLGCWGLIQWQESRIAKNILTIREQENTLAKLEAKTWGVTFVNGENGKFLVL
                     PDGVKGENTWTVGDRNAVRLVRE"
                     /name="MbeB family mobilization protein"
     gene            916..1149
                     /locus_tag="JSU11_RS24400"
                     /old_locus_tag="JSU11_24405"
     CDS             916..1149
                     /locus_tag="JSU11_RS24400"
                     /old_locus_tag="JSU11_24405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_001522451.1"
                     /translation="MTELEGHLLNALEHLQQDYMRRLSEWESAFAELQKMHAVTQQNNA
                     ILNERVVNLSQQVQLLAGQVDRLSRLFITNNR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(1601..2848)
                     /locus_tag="JSU11_RS24405"
                     /old_locus_tag="JSU11_24410"
     CDS             complement(1601..2848)
                     /locus_tag="JSU11_RS24405"
                     /old_locus_tag="JSU11_24410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001522448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="WP_001522448.1"
                     /translation="MIKFVDLFSGTGGIRLGFEQAMKEIGLSTKCVKSSEIDKKACETY
                     KLNFDEESYCDIHDVDIDESFDVLLAGFPCQAFSYAGKQLGFADTRGTLFFEVERIIKK
                     NKPKLCLLENVRGLTSHDKGRTFSTILKSLHDLGYHVEYRLLNTSNFGVPQNRVRIYII
                     ASLKKEVTLTIPNDLGAKDTHNYKPSKGGHDFYTESKVSDILEDSPDKSYDCSDWFKQR
                     LEDIFKGDLNKVHGIRLIDTRNGNSLHSWDLGLKGECSNDEIAFMNSLIANRRKHIFGT
                     HQDGKALSKAQIETFFKHSETTNIIKNLIKKGYLKEKNGLYNPVCGNMSFEVFKFLDPN
                     KVSITLTASDANRLGVYHNNRLRRLTPRECARLQGYPDSYILHPKDSMAYKQLGNAVSV
                     PVIKNIFLDIMKNNFI"
                     /name="DNA cytosine methyltransferase"
     gene            complement(2845..3855)
                     /locus_tag="JSU11_RS24410"
                     /old_locus_tag="JSU11_24415"
     CDS             complement(2845..3855)
                     /locus_tag="JSU11_RS24410"
                     /old_locus_tag="JSU11_24415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000395670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001522447.1"
                     /translation="MEFQELENSACLYWPKELAERAASISAISPLIETQDEFLSILTIS
                     TNKPTSCFDAVRLCNKISPNLFVKHLMVLSDIGGERLHRFFKDLDKIYPDRIMEFNIGN
                     NSYSYQFNSNRAWTTKNLNVEKSRLLQPVSDFTREMLDVCMLILWGGNTINNTNLPTEI
                     ENNCILGNLIGNKEAIEQFVKERYIMVSRQTGGATANDLGHICEIFIKEKLYKLIDDNI
                     SLDGHHIDGVTHNDKDLTTFDIVAKNTITKKCVGIEISFQVTTNSTIERKAGLAKSRRD
                     LLNSIGHKIAYVIDGSGNFQRSNAIKTIINHSDMTVNFSDNGINSLATFICETLK"
                     /name="hypothetical protein"
     gene            complement(4083..4214)
                     /locus_tag="JSU11_RS25080"
     CDS             complement(4083..4214)
                     /locus_tag="JSU11_RS25080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001318243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_071791513.1"
                     /translation="MHEPPVQPDRCALSGNCRLESNPERHAKAPLAAAIGRGCTRNC"
                     /name="replication initiation protein"
     gene            complement(4883..5074)
                     /locus_tag="JSU11_RS24415"
                     /old_locus_tag="JSU11_24420"
     CDS             complement(4883..5074)
                     /locus_tag="JSU11_RS24415"
                     /old_locus_tag="JSU11_24420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165985.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            5125..5463
                     /locus_tag="JSU11_RS24420"
                     /old_locus_tag="JSU11_24425"
     CDS             5125..5463
                     /locus_tag="JSU11_RS24420"
                     /old_locus_tag="JSU11_24425"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_320406752.1"
                     /translation="MKRERMLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSG
                     KLPTLAPPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVVAALMTIERELRSLRQVVR
                     EQGARDDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     Typing          4713..4798
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="98.851"
                     /coverage="100.0"
                     /evalue="3.99e-36"
     Typing          complement(4143..4700)
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.107"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          4..1247
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="95.663"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          5454..5791
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.115"
                     /coverage="99.0"
                     /evalue="1.23e-67"
ORIGIN
        1 tacagagcgg aaaacgcctc cagccgtact acgaccgggc agacaggccg agaattgatg
       61 cctggcagac gctggttaat catcattacg ggctgcgtga cccgaacgca ccggagaaca
      121 gacggacgct tgtcacgcca aataatctgc caaaagcgaa acaggaggcc gcagaggcga
      181 ttacacgggg tcttgaggct ctttaccatg ccggagcaat aaaaacgcgt caggatgtca
      241 cagaggcgct tacagcggca ggttttgagg tggtgaggac aaccaaaagc agcataagta
      301 ttgccgaccc ggaggggggg cggaatctca ggctaaaggg ggcgctgtat gagcaatctt
      361 ttacaaatgg gaacagactt cgagaagaaa ctcaaagagc gagccgcttc taccgagaac
      421 atgctgaaca gcgagttcag gaagctcgaa gagtctgtag acaaggcact gagcttaaac
      481 aggcagaaaa tcagagacgc tatcagcgaa catacaacat ccgtgaaaca gcaactggat
      541 acgctgagca ccaccgtgag cacgcagttc agcaccacag aggccgaact gtcccggcag
      601 cagaaaaaac tgctctggca ggtgatcaaa gggcggatac tgtttccggc gctgaccgcg
      661 ctcagcgtca cgggcggcat tttcctggga tgctgggggc tgattcagtg gcaggaaagc
      721 aggattgcga aaaatatcct gactatcaga gagcaggaaa acacgctggc gaaactcgag
      781 gcaaaaacct ggggggtgac gttcgtcaat ggcgagaacg ggaaattcct ggtgctgccg
      841 gacggggtga aaggggaaaa cacctggacg gtgggcgaca ggaatgccgt gagactggtc
      901 agggagtaaa cgagtatgac ggaactggaa gggcatttgc tgaacgcctt agagcatttg
      961 cagcaggact atatgcggcg gctgagcgag tgggagagcg ccttcgcgga attgcagaag
     1021 atgcacgcgg ttacgcaaca gaacaacgcg atactgaacg agcgtgtcgt gaacttgagt
     1081 cagcaggtgc agctattagc cggacaagta gaccgcttga gtcggttatt tatcacgaac
     1141 aacagataag ggatgaacag gagcgtcagg cgcaggagct tgccctgcgc tggcatgaag
     1201 agctggaacc cgaagcgcgg atctggcgtg gcccgtcaat ggggatgtga ccggggtgtc
     1261 acctgaaatc gtgccagttt gtggcacgct ctacccggat gacgccaatg ctaatgtatg
     1321 cataattagc attaacaata tccaaacttc cccattttac cccgttcgac ggctggaacg
     1381 tgcgaaactg atcgtcattc agtttcgcgc ataactatgc atgaggttaa aatttaccag
     1441 gcgcgatcgc ggcagttttt cgggtggttt gttgccattt ttacctgtct gctgccgtga
     1501 tcgcgctgaa cgcgttttag cggtgcgcgt aatgcgacat tatggtaaat ctgttaaatt
     1561 taattaaata accatagagc agttttgcta gtaatatagt ttatatgaag ttattcttca
     1621 ttatatcaag aaaaatattt ttaatgactg gaacgcttac agcattcccc aattgtttat
     1681 atgccatgct atcttttggg tgtaagatat atgagtctgg gtaaccttgt agccttgcac
     1741 attctcttgg tgtaaggcgt cgcagtctat tgttgtgata gactccaagt ctattggcat
     1801 cagatgcagt aagtgtaatt gacactttat ttgggtcaag gaatttgaat acttcaaaag
     1861 acatatttcc gcaaactggg ttgtatagtc cgtttttttc tttaaggtaa ccctttttaa
     1921 ttagattttt tattatgttg gtggtttcgc tatgtttgaa aaatgtttca atttgtgctt
     1981 ttgacaaagc cttgccgtct tggtgtgtgc caaaaatatg ctttctacga tttgcaatta
     2041 atgaattcat gaaagctatt tcatcatttg agcactcgcc ttttagccca agatcccaag
     2101 agtgcaagga gttgccgttc cttgtgtcaa ttagcctgat tccatgaact ttattcaggt
     2161 cacctttaaa aatatcttca aggcgttgct tgaaccaatc gctgcaatca tatgacttgt
     2221 ctggactatc ctctagtatg tcagaaactt ttgattctgt ataaaaatca tgaccacctt
     2281 tggatggttt gtaattgtgc gtatctttgg ctccaagatc attaggaata gtaagcgtta
     2341 cctctttttt taatgaggct attatatata ttctaaccct attttgaggg acgccaaaat
     2401 tacttgtatt taatagtctg tattcaacat gataccctaa atcgtgaagg gattttaata
     2461 ttgttgaaaa tgtccttcct ttatcatgtg aagttaatcc acgtacattc tccaaaagac
     2521 acaatttagg cttgtttttc tttataattc tttctacttc aaaaaataat gtacctcggg
     2581 tgtctgcaaa accaagttgt tttccagcat aagagaatgc ttggcaagga aatcctgcca
     2641 ataaaacatc gaagctttca tctatatcaa catcatgtat atcacaatat gattcttcgt
     2701 cgaagttaag tttgtatgtt tcgcatgctt ttttgtcaat ttctgagctt tttacacatt
     2761 ttgttgacaa tccaatttct ttcatggctt gctcaaaacc aagtctaata ccacctgtgc
     2821 cagaaaaaag gtcaacaaat ttaatcattt caatgtctcg cagatgaatg ttgctagtga
     2881 atttatacca ttatctgaaa agttaacagt catgtcacta tggttaatta ttgtttttat
     2941 ggcatttgag cgctgaaaat taccagaccc atcaatcaca tatgctattt tatgccctat
     3001 tgaattgagt aaatctcttc tgcttttggc taatcctgct tttctttcta ttgttgagtt
     3061 tgttgtaact tgaaaactta tttcaatacc aacacatttt tttgttatcg tgttttttgc
     3121 tacaatatca aaagtcgtaa ggtctttatc gttatgagtt acaccatcga tatggtgtcc
     3181 atctagagat atattatcat caataagttt ataaagtttt tcttttataa agatctcgca
     3241 tatgtgtcca agatcatttg ccgttgcacc tcctgtttgt ctgctaacca ttatatatct
     3301 ttcttttaca aattgttcta ttgcttcttt gttcccaatt aaatttccca aaatacagtt
     3361 attttctatt tcagttggaa ggtttgtatt gtttatagtg ttacctcccc acaggatcag
     3421 catgcaaaca tcaagcattt ctcgtgtaaa atcacttaca ggttgcaaaa gtcgtgattt
     3481 ttccacgttt aaattcttgg ttgtccatgc tctattgcta ttgaattggt aagagtaaga
     3541 gttattccct atattgaact ccattattct gtcaggatag attttatcta aatctttaaa
     3601 aaagcgatga agtctttcgc ctccaatatc tgacaaaacc attaggtgct tgacaaatag
     3661 gttgggtgat atcttattgc ataaccgtac agcatcaaaa caagatgtag gcttatttgt
     3721 tgatattgtt agtattgata aaaattcatc ttgggtctct ataagagggc ttatggcgct
     3781 aatagatgct gctctttctg caagttcttt gggccagtat aagcatgcgc tattttctaa
     3841 ttcttggaac tccatgatta ttccttggta aaactatcca aatagacctt gagttacacc
     3901 cctatgatgg gtaatctcta cgggcgtaaa cgaaaaaacc cacgttctca ggcgtgggtt
     3961 tttcgaaggt tagaagtttc tcaggctttc taacccaagg taacagggcg tacaagaccg
     4021 ctgccaccaa atctgtcctt tcagtgtagc cgcagtcggg ccttcacttc aagaactccg
     4081 tatcagcaat ttcttgtaca ccctctacca atggctgctg ccagtggtgc ttttgcatgt
     4141 ctttccgggt tggactcaag acgacagtta ccggataagg cgcagcggtc gggctgaacg
     4201 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccaa
     4261 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg
     4321 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg
     4381 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcggtt tttgtgatgc
     4441 tcgtcagggg ggcggagcct atggaaaaac gccagcggcg tggccttttc cgggttctcg
     4501 ttttttgctc acatgtttct tttggcgtta tcccctgatt ctgtggataa ccgcatctcc
     4561 gcttttgagt gagccgacac cgctcgccgc agccgaacga ccgagcgtag cgagtcagtg
     4621 agcgaggaag cggaagagcg ccggaacgtg cattttctcc ttacgcatct gtgcggaatt
     4681 tcacacccgg catggggcac ttttcataca atctgcattg atgccgcata gttaagccag
     4741 tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgctaaaac
     4801 ctgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgctcacagg caagctgtga
     4861 ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc
     4921 agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat
     4981 ccgcgtccag ttcgttgagt ttctccagca gcgttaatgt ctggcttctg ataaagcggg
     5041 ccatgttaag ggcggttttt tcctgtttag tcactgatgc ctccgtgtaa ggggggattt
     5101 ctgttcatgg gggtaatgat accgatgaaa cgcgagagga tgctcacgat acgggttact
     5161 gatgatgaac atgcccggtt actggaacgc tgtgagggta aacaactggc ggtatggatg
     5221 cggcgggtct gcctgggtga gccggttgcc cgttccggga agctgccgac actggcaccg
     5281 ccgttactgc gtcagctggc tgccatcggg aataacctga atcagacggc gaggaaggtg
     5341 aacagcgggc agtggtcttc cggcgaccgg gttcaggtgg tggccgcact gatgaccatc
     5401 gagagagagt tgcgcagctt acggcaggtc gtcagggaac agggagcgcg ggatgatagt
     5461 taagtttcat gcccggggaa aaggtggtgg cagtggtccg gttgattacc ttctggggag
     5521 ggagcgtaac cgggaaggtg caacggtact tcagggtaac ccggaagaag tccgggagct
     5581 tattgatgcc acgccatttg cgaagaaata cacgtccggt gttctgtcgt tcgcagagaa
     5641 ggaactgccg ccgggaggac gtgaaaaagt gatggagagc tttgagcgcg tactgatgcc
     5701 cggtctcgaa aaaaatcagt acagcattct gtgggtggag caccaggaca aggggcgtct
     5761 tgagctgaat tttgtcattc cgaacatgga gc
//
