LOCUS       NZ_CP069674             2089 bp    DNA     circular CON 18-JUN-2023
DEFINITION  Escherichia coli O89m:H9 strain MIN11 plasmid pMUB-MIN11-7, complete
            sequence.
ACCESSION   NZ_CP069674
VERSION     NZ_CP069674.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN17831484
            Assembly: GCF_017570625.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli O89m:H9
  ORGANISM  Escherichia coli O89m:H9
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2089)
  AUTHORS   Majewski,P.
  TITLE     Plasmid mediated mcr-1.1 colistin-resistance in clinical
            extraintestinal Escherichia coli strains isolated in Poland
  JOURNAL   Front Microbiol (2021) In press
REFERENCE   2  (bases 1 to 2089)
  AUTHORS   Majewski,P.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2021) Department of Microbiological Diagnostics
            and Infectious Immunology, Medical University of Bialystok,
            Waszyngtona 15A, Bialystok 15-269, Poland
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: Unicycler hybrid assembly v. v0.4.8-beta
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100x
            Sequencing Technology :: Illumina MiSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 06/18/2023 02:04:53
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,884
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 121
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 13
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 76 of 240
            Pseudo Genes (incomplete)         :: 162 of 240
            Pseudo Genes (internal stop)      :: 55 of 240
            Pseudo Genes (multiple problems)  :: 51 of 240
            Pseudo Genes (short protein)      :: 4 of 240
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP069674.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2089
                     /organism="Escherichia coli O89m:H9"
                     /mol_type="genomic DNA"
                     /strain="MIN11"
                     /serotype="O89m:H9"
                     /isolation_source="lower respiratory tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:2810406"
                     /plasmid="pMUB-MIN11-7"
                     /geo_loc_name="Poland: Bialystok"
                     /lat_lon="53.12523570 N 23.15905989 E"
                     /collection_date="2018-03-29"
                     /genotype="ST-10"
     gene            join(1374..2089,1..250)
                     /locus_tag="JSU13_RS24045"
                     /old_locus_tag="JSU13_24030"
     CDS             join(1374..2089,1..250)
                     /locus_tag="JSU13_RS24045"
                     /old_locus_tag="JSU13_24030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012421200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="WP_001287824.1"
                     /translation="MSEDKFLSDYSPRDAVWDTQRTLTDSVGGIYQTAAEFERYALRMA
                     SCSGLLRFGWSTIMETGETRLRLRSAQFCRVRHCPVCQWRRTLMWQARFYQALPKIVVD
                     YPSSRWLFLTLTVRNCEIGELGTVLTAMNAAFKRMEKRKELSPVQGWIRATEVTRGKDG
                     SAHPHFHCLLMVQPSWFKGKNYVKHERWVELWRDCLRVNYEPNIDIRAVKTKTGEVVAN
                     VAEQLQSAVAETLKYSVKPEDMANDPEWFLELTRQLHKRRFISTGGALKNVLQLDRETN
                     EDLVIADDVGDGTDDGKRTAFVWDSGKRRYKRAPEKDKSD"
                     /name="protein rep"
     gene            254..520
                     /locus_tag="JSU13_RS24050"
                     /old_locus_tag="JSU13_24035"
     CDS             254..520
                     /locus_tag="JSU13_RS24050"
                     /old_locus_tag="JSU13_24035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001024536.1"
                     /translation="MNINIEYLNGNKTIGLFFLRSEAVIPDRFKNLILLIDGLSFGTFG
                     FHPHEGFEDELILYIQKTNERVKTLFVKIDLNGIKFGFLRTHS"
                     /name="hypothetical protein"
     Typing          1768..1999
                     /name="Col(BS512)"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="2.05e-118"
ORIGIN
        1 taaaccggaa gatatggcaa acgatcctga gtggtttctt gagctgacgc ggcagcttca
       61 caagcgccgt tttatctcga ccggtggggc gctaaaaaac gtcctccagt tggatcgaga
      121 aaccaatgag gatcttgtca ttgccgacga tgtaggggat ggcactgatg acgggaagcg
      181 gacggcgttt gtctgggatt caggtaaacg gcgttacaaa cgcgcccctg agaaggataa
      241 atcggattaa cgtatgaata ttaatattga atacctgaat ggaaataaga ctattggttt
      301 atttttttta agaagtgaag cggtgattcc tgacaggttt aaaaacctta ttttgcttat
      361 tgatggatta agttttggca catttggttt tcatccgcac gaaggttttg aggatgaatt
      421 aattttatat attcagaaaa caaacgagag ggtaaaaact ctttttgtga aaatagattt
      481 aaacggtatc aagtttggat ttttaagaac gcattcttag ttctggaaaa gagccagcgg
      541 caggctgagg tgataggtac gagattgcat gcaatctcta gtgctctgtc tatcctgcat
      601 tatcctcagc attatcctca gccttgccaa ctcgacacca atgcaggata gacaatccga
      661 tgtcaaatgt taacactctg cgagtggtac attttccccg gattatcgtc ctgagcctgc
      721 cgctggctct ctttctaccg cctcgctttg ctcgttgctc aacgcctcac agacacggat
      781 taaaatccgc atccgttcac cgttttttaa agtccgttaa aagcatgatg ccatctccga
      841 gagttaatct cgtcaaatgc taaatcgtgg gggtcccctt tggggttccg atttagtgat
      901 tgacgacacc accgattaaa aaacttatgc ggggtggatg gtttcacgaa gtgaggccat
      961 ccacctgtaa gacagggttt tgtttttatt ccctgttttg gtgatcgggt gtgtggaaaa
     1021 ggttggggta agccgttcgg gggtgcttgt tttggggggt taaaattgtg gttatttttt
     1081 gcgcaattct cgcgcgtgat ccttgtattt atacttaagg gataaatggc ggatatgaaa
     1141 tagtggttta gcccagtaat gacgaggctt tgagtgggtt ttgacaggtc aaagaaaatg
     1201 gagcagaatt gaggcgtttt taatcggcgt tggggagtgc gtcaacactc cccaacattt
     1261 cgaatgtgtc acctcagcgg caaactctgg tgacatgtac tggctcgcaa tgcacaggta
     1321 cgtgatgaat ataccacatc aaatcacagc ctgcccagat cggagcaggc ttaatgtcag
     1381 aagataaatt cctttcggac tacagccccc gtgatgcagt ttgggatacc cagcgcacgc
     1441 ttaccgattc tgtcgggggt atctaccaga ctgctgctga attcgagcgc tatgcactcc
     1501 gtatggcctc ctgtagcggt ttgttacgtt ttggttggtc taccatcatg gaaaccggag
     1561 aaacgcgcct acggcttcgt agtgcgcaat tttgccgtgt ccgtcattgc cctgtctgcc
     1621 agtggagaag aaccctcatg tggcaagccc gtttttatca ggctctaccg aaaatcgttg
     1681 tggattaccc gtcttcccga tggttgtttc tgacgttaac tgtcaggaac tgcgagatag
     1741 gtgaacttgg aacagtcctt acagcaatga atgcggcgtt taagcgaatg gaaaagcgaa
     1801 aggagctatc acctgttcag gggtggatca gggctacgga ggtgacgcga ggtaaggatg
     1861 gcagcgcaca tccgcatttt cactgtctgc tgatggtgca accttcttgg tttaaaggga
     1921 agaactacgt taagcacgaa cgttgggtag aactctggcg cgattgcttg cgggtgaact
     1981 atgagccgaa tatcgatatt cgggcagtaa aaactaagac aggtgaggtt gtggccaacg
     2041 ttgccgagca actgcaaagc gcggttgctg aaacgctgaa atactccgt
//
