LOCUS       NZ_CP069673             4286 bp    DNA     circular CON 18-JUN-2023
DEFINITION  Escherichia coli O89m:H9 strain MIN11 plasmid pMUB-MIN11-6, complete
            sequence.
ACCESSION   NZ_CP069673
VERSION     NZ_CP069673.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN17831484
            Assembly: GCF_017570625.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli O89m:H9
  ORGANISM  Escherichia coli O89m:H9
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4286)
  AUTHORS   Majewski,P.
  TITLE     Plasmid mediated mcr-1.1 colistin-resistance in clinical
            extraintestinal Escherichia coli strains isolated in Poland
  JOURNAL   Front Microbiol (2021) In press
REFERENCE   2  (bases 1 to 4286)
  AUTHORS   Majewski,P.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2021) Department of Microbiological Diagnostics
            and Infectious Immunology, Medical University of Bialystok,
            Waszyngtona 15A, Bialystok 15-269, Poland
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: Unicycler hybrid assembly v. v0.4.8-beta
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100x
            Sequencing Technology :: Illumina MiSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 06/18/2023 02:04:53
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,884
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 121
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 13
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 76 of 240
            Pseudo Genes (incomplete)         :: 162 of 240
            Pseudo Genes (internal stop)      :: 55 of 240
            Pseudo Genes (multiple problems)  :: 51 of 240
            Pseudo Genes (short protein)      :: 4 of 240
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP069673.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4286
                     /organism="Escherichia coli O89m:H9"
                     /mol_type="genomic DNA"
                     /strain="MIN11"
                     /serotype="O89m:H9"
                     /isolation_source="lower respiratory tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:2810406"
                     /plasmid="pMUB-MIN11-6"
                     /geo_loc_name="Poland: Bialystok"
                     /lat_lon="53.12523570 N 23.15905989 E"
                     /collection_date="2018-03-29"
                     /genotype="ST-10"
     gene            complement(join(4193..4286,1..1154))
                     /locus_tag="JSU13_RS24030"
                     /old_locus_tag="JSU13_24015"
     CDS             complement(join(4193..4286,1..1154))
                     /locus_tag="JSU13_RS24030"
                     /old_locus_tag="JSU13_24015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001522448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="WP_001522448.1"
                     /translation="MIKFVDLFSGTGGIRLGFEQAMKEIGLSTKCVKSSEIDKKACETY
                     KLNFDEESYCDIHDVDIDESFDVLLAGFPCQAFSYAGKQLGFADTRGTLFFEVERIIKK
                     NKPKLCLLENVRGLTSHDKGRTFSTILKSLHDLGYHVEYRLLNTSNFGVPQNRVRIYII
                     ASLKKEVTLTIPNDLGAKDTHNYKPSKGGHDFYTESKVSDILEDSPDKSYDCSDWFKQR
                     LEDIFKGDLNKVHGIRLIDTRNGNSLHSWDLGLKGECSNDEIAFMNSLIANRRKHIFGT
                     HQDGKALSKAQIETFFKHSETTNIIKNLIKKGYLKEKNGLYNPVCGNMSFEVFKFLDPN
                     KVSITLTASDANRLGVYHNNRLRRLTPRECARLQGYPDSYILHPKDSMAYKQLGNAVSV
                     PVIKNIFLDIMKNNFI"
                     /name="DNA cytosine methyltransferase"
     gene            complement(1151..2161)
                     /locus_tag="JSU13_RS24035"
                     /old_locus_tag="JSU13_24020"
     CDS             complement(1151..2161)
                     /locus_tag="JSU13_RS24035"
                     /old_locus_tag="JSU13_24020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000395670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001522447.1"
                     /translation="MEFQELENSACLYWPKELAERAASISAISPLIETQDEFLSILTIS
                     TNKPTSCFDAVRLCNKISPNLFVKHLMVLSDIGGERLHRFFKDLDKIYPDRIMEFNIGN
                     NSYSYQFNSNRAWTTKNLNVEKSRLLQPVSDFTREMLDVCMLILWGGNTINNTNLPTEI
                     ENNCILGNLIGNKEAIEQFVKERYIMVSRQTGGATANDLGHICEIFIKEKLYKLIDDNI
                     SLDGHHIDGVTHNDKDLTTFDIVAKNTITKKCVGIEISFQVTTNSTIERKAGLAKSRRD
                     LLNSIGHKIAYVIDGSGNFQRSNAIKTIINHSDMTVNFSDNGINSLATFICETLK"
                     /name="hypothetical protein"
     gene            complement(3184..3378)
                     /locus_tag="JSU13_RS24040"
                     /old_locus_tag="JSU13_24025"
     CDS             complement(3184..3378)
                     /locus_tag="JSU13_RS24040"
                     /old_locus_tag="JSU13_24025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_001041631.1"
                     /translation="MNKQQQTALNMARFIKSQSLTLLEKLDALDADEQAAMCERLHELA
                     EELQNSIQIRFEAESETGT"
                     /name="Rop family plasmid primer RNA-binding protein"
     Typing          3019..3104
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="8.58e-38"
     Typing          3415..3513
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="98.0"
                     /coverage="100.0"
                     /evalue="1.35e-41"
     Typing          3638..3893
                     /name="MOBF"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="96.899"
                     /coverage="89.0"
                     /evalue="2.61e-118"
     Typing          complement(2449..2997)
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.414"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 tttgggtgta agatatatga gtctgggtaa ccttgtagcc ttgcacattc tcttggtgta
       61 aggcgtcgca gtctattgtt gtgatagact ccaagtctat tggcatcaga tgcagtaagt
      121 gtaattgaca ctttatttgg gtcaaggaat ttgaatactt caaaagacat atttccgcaa
      181 actgggttgt atagtccgtt tttttcttta aggtaaccct ttttaattag attttttatt
      241 atgttggtgg tttcgctatg tttgaaaaat gtttcaattt gtgcttttga caaagccttg
      301 ccgtcttggt gtgtgccaaa aatatgcttt ctacgatttg caattaatga attcatgaaa
      361 gctatttcat catttgagca ctcgcctttt agcccaagat cccaagagtg caaggagttg
      421 ccgttccttg tgtcaattag cctgattcca tgaactttat tcaggtcacc tttaaaaata
      481 tcttcaaggc gttgcttgaa ccaatcgctg caatcatatg acttgtctgg actatcctct
      541 agtatgtcag aaacttttga ttctgtataa aaatcatgac cacctttgga tggtttgtaa
      601 ttgtgcgtat ctttggctcc aagatcatta ggaatagtaa gcgttacctc tttttttaat
      661 gaggctatta tatatattct aaccctattt tgagggacgc caaaattact tgtatttaat
      721 agtctgtatt caacatgata ccctaaatcg tgaagggatt ttaatattgt tgaaaatgtc
      781 cttcctttat catgtgaagt taatccacgt acattctcca aaagacacaa tttaggcttg
      841 tttttcttta taattctttc tacttcaaaa aataatgtac ctcgggtgtc tgcaaaacca
      901 agttgttttc cagcataaga gaatgcttgg caaggaaatc ctgccaataa aacatcgaag
      961 ctttcatcta tatcaacatc atgtatatca caatatgatt cttcgtcgaa gttaagtttg
     1021 tatgtttcgc atgctttttt gtcaatttct gagcttttta cacattttgt tgacaatcca
     1081 atttctttca tggcttgctc aaaaccaagt ctaataccac ctgtgccaga aaaaaggtca
     1141 acaaatttaa tcatttcaat gtctcgcaga tgaatgttgc tagtgaattt ataccattat
     1201 ctgaaaagtt aacagtcatg tcactatggt taattattgt ttttatggca tttgagcgct
     1261 gaaaattacc agacccatca atcacatatg ctattttatg ccctattgaa ttgagtaaat
     1321 ctcttctgct tttggctaat cctgcttttc tttctattgt tgagtttgtt gtaacttgaa
     1381 aacttatttc aataccaaca catttttttg ttatcgtgtt ttttgctaca atatcaaaag
     1441 tcgtaaggtc tttatcgtta tgagttacac catcgatatg gtgtccatct agagatatat
     1501 tatcatcaat aagtttataa agtttttctt ttataaagat ctcgcatatg tgtccaagat
     1561 catttgccgt tgcacctcct gtttgtctgc taaccattat atatctttct tttacaaatt
     1621 gttctattgc ttctttgttc ccaattaaat ttcccaaaat acagttattt tctatttcag
     1681 ttggaaggtt tgtattgttt atagtgttac ctccccacag gatcagcatg caaacatcaa
     1741 gcatttctcg tgtaaaatca cttacaggtt gcaaaagtcg tgatttttcc acgtttaaat
     1801 tcttggttgt ccatgctcta ttgctattga attggtaaga gtaagagtta ttccctatat
     1861 tgaactccat tattctgtca ggatagattt tatctaaatc tttaaaaaag cgatgaagtc
     1921 tttcgcctcc aatatctgac aaaaccatta ggtgcttgac aaataggttg ggtgatatct
     1981 tattgcataa ccgtacagca tcaaaacaag atgtaggctt atttgttgat attgttagta
     2041 ttgataaaaa ttcatcttgg gtctctataa gagggcttat ggcgctaata gatgctgctc
     2101 tttctgcaag ttctttgggc cagtataagc atgcgctatt ttctaattct tggaactcca
     2161 tgattattcc ttggtaaaac tatccaaata gaccttgagt tacaccccta tgatgggtaa
     2221 tctctacggg cgtaaacgaa aaaacccacg ttctcaggcg tgggtttttc gaaggttaga
     2281 agtttctcag gctttctaac ccaaggtaac agggcgtaca agaccgctgc caccaaatct
     2341 gtcctttcag tgtagccgca gtcgggcctt cacttcaaga actccgtatc agcaatttct
     2401 tgtacaccct ctaccaatgg ctgctgccag tggtgctttt gcatgtcttt ccgggttgga
     2461 ctcaagacga cagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
     2521 acagcccagc ttggagcgaa cgacctacac cgaactgaga taccaacagc gtgagctatg
     2581 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt
     2641 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc
     2701 tgtcgggttt cgccacctct gacttgagcg tcggtttttg tgatgctcgt caggggggcg
     2761 gagcctatgg aaaaacgcca gcgacgtggc cttttccggg ttctcgtttt ttgctcacat
     2821 gtttcttttg gcgttatccc ctgattctgt ggataaccgc atctccgctt ttgagtgagc
     2881 cgacaccgct cgccgcagcc gaacgaccga gcgtagcgag tcagtgagcg aggaagcgga
     2941 agagcgccgg aacgtgcatt ttctccttac gcatctgtgc ggcatttcac accgcatatg
     3001 gcgctctttt cttacaatct gcactgatgc cgcatggtta agccagtata cactccgcta
     3061 tcgctacgtg actgggtcat ggctgcgccc cgacacccgc taaaaccccc tgacgcgccc
     3121 tgacgggctt gtctgctgac gaaaacgccc acgggaaagc cgtgaccgcc tccgtcagct
     3181 tcgttatgtt cctgtttcac tttcggcctc aaagcgaatc tggatgctgt tctggagttc
     3241 ttctgcgagt tcgtgcagtc gttcgcacat ggccgcctgt tcgtcggcat ccagagcatc
     3301 cagtttttca agcagtgtca ggctctggct tttgataaat cgcgccatat tcagcgcagt
     3361 ttgttgttgt ttgttcacct tccgttgttc tccgtcagtt ccgggtgtcg gggggaccct
     3421 gagcaggtgg taattgtaag acgttgcggg cgacggtatt acaattgcac atcctgtcct
     3481 gtttctttag gacgtatcat ggcacagcat atcaactgat aaataatccc tgtgcagtgc
     3541 agccgctgtt gcataaaatt tcgctctgcg tcgagtgttt cttaatcaat aattccgctg
     3601 atactccgga aaaacgcgag tctgttctca tgcctgaaat gcccacaccc cacgcaaaaa
     3661 caagtttttg ctgatttttc tttataaata gagagttatg acaaattagt tcttcttgct
     3721 ctctttgtga tatttaaaaa agcggtgtcg gcgcggtgtt gtagctgcgc caacaccgct
     3781 ttttaggggt ggtactgact attttcataa aaaacatcat cttatattag gggtgctgct
     3841 agcggcgcgg tgtgtttttt tacaggacac ccctgggggc gctgctaggg gtgtctgttc
     3901 agatatgtag caatgagtca gggattcacg aactgaaaat gtccgaaaat gtgtctcatg
     3961 ggcactgtat gcgcccaaac cgatcgtcat tcactttcat gcatagctat gcagtgagct
     4021 gaaagcgatc ctgacgcatt tttccggttt accccgggga gaacatctct ttttgcggtg
     4081 ttcgcggcag aatcgctttc agcgcgtttt agcggtgcgc gcaatgcgac attatggtaa
     4141 atctgttaaa tttaattaaa taaccataga gcagttttgc tagtaatata gtttatatga
     4201 agttattctt cattatatca agaaaaatat ttttaatgac tggaacgctt acagcattcc
     4261 ccaattgttt atatgccatg ctatct
//
