LOCUS       NZ_CP069672             5433 bp    DNA     circular CON 18-JUN-2023
DEFINITION  Escherichia coli O89m:H9 strain MIN11 plasmid pMUB-MIN11-5, complete
            sequence.
ACCESSION   NZ_CP069672
VERSION     NZ_CP069672.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN17831484
            Assembly: GCF_017570625.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli O89m:H9
  ORGANISM  Escherichia coli O89m:H9
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5433)
  AUTHORS   Majewski,P.
  TITLE     Plasmid mediated mcr-1.1 colistin-resistance in clinical
            extraintestinal Escherichia coli strains isolated in Poland
  JOURNAL   Front Microbiol (2021) In press
REFERENCE   2  (bases 1 to 5433)
  AUTHORS   Majewski,P.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2021) Department of Microbiological Diagnostics
            and Infectious Immunology, Medical University of Bialystok,
            Waszyngtona 15A, Bialystok 15-269, Poland
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: Unicycler hybrid assembly v. v0.4.8-beta
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100x
            Sequencing Technology :: Illumina MiSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 06/18/2023 02:04:53
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,884
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 121
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 13
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 76 of 240
            Pseudo Genes (incomplete)         :: 162 of 240
            Pseudo Genes (internal stop)      :: 55 of 240
            Pseudo Genes (multiple problems)  :: 51 of 240
            Pseudo Genes (short protein)      :: 4 of 240
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP069672.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5433
                     /organism="Escherichia coli O89m:H9"
                     /mol_type="genomic DNA"
                     /strain="MIN11"
                     /serotype="O89m:H9"
                     /isolation_source="lower respiratory tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:2810406"
                     /plasmid="pMUB-MIN11-5"
                     /geo_loc_name="Poland: Bialystok"
                     /lat_lon="53.12523570 N 23.15905989 E"
                     /collection_date="2018-03-29"
                     /genotype="ST-10"
     gene            complement(join(5218..5433,1..369))
                     /locus_tag="JSU13_RS23990"
                     /old_locus_tag="JSU13_23975"
     CDS             complement(join(5218..5433,1..369))
                     /locus_tag="JSU13_RS23990"
                     /old_locus_tag="JSU13_23975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000130481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel transporter"
                     /protein_id="WP_249544517.1"
                     /translation="MALENMIRFRDTIERLGGSMAKEFDVETKGKRTYSMNNDMSDLYN
                     VISRAVEASGADHSINEKLTNVLKRELVDYVSIAHLKTKLSVLYEFEKNYLQLIAEYKE
                     EIKFASSLQEDLRKERAKFFSETLKEVHQTLNESQVDNEVASKWIKELVGSYTKSLDLS
                     GGLVEEHTLDTIACIRAEAKLNKPSIEPGNN"
                     /name="nickel transporter"
     gene            complement(729..962)
                     /locus_tag="JSU13_RS23995"
                     /old_locus_tag="JSU13_23980"
     CDS             complement(729..962)
                     /locus_tag="JSU13_RS23995"
                     /old_locus_tag="JSU13_23980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_208207794.1"
                     /translation="MTDLERHLLNALEQLQQDYMQRLNEWESAFVELQKMFSLTQRDNA
                     MLNERVMQLSQQVQHLSEQTERLSQLYSENWR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(969..1487)
                     /locus_tag="JSU13_RS24000"
                     /old_locus_tag="JSU13_23985"
     CDS             complement(969..1487)
                     /locus_tag="JSU13_RS24000"
                     /old_locus_tag="JSU13_23985"
                     /inference="COORDINATES: protein motif:HMM:NF016710.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_023167411.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTEKMLNNEFRRLGESVSEAVTSNE
                     TKIRDAIALFTTSTEESLKKHREGVKEAMMQHRKDVLKLAGNTGVMLLGIVFLLFTASG
                     GTLWYLGGRIQANLEEIRIQEETLQKLNAKTWGVEFVQDGNRKFLVIPQGKSATVIPYQ
                     GKDWVQLTE"
                     /name="MbeB family mobilization protein"
     gene            complement(<1525..1956)
                     /locus_tag="JSU13_RS24425"
                     /pseudo=""
     CDS             complement(<1525..1956)
                     /locus_tag="JSU13_RS24425"
                     /inference="COORDINATES: protein motif:HMM:NF015397.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(2165..2488)
                     /locus_tag="JSU13_RS24010"
                     /old_locus_tag="JSU13_23995"
     CDS             complement(2165..2488)
                     /locus_tag="JSU13_RS24010"
                     /old_locus_tag="JSU13_23995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_000955989.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRICLGEPVARSGKLPTL
                     APPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVVAALMAIERELRSLRQVVREHGAR
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            2553..2744
                     /locus_tag="JSU13_RS24015"
                     /old_locus_tag="JSU13_24000"
     CDS             2553..2744
                     /locus_tag="JSU13_RS24015"
                     /old_locus_tag="JSU13_24000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165985.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(3886..4689)
                     /locus_tag="JSU13_RS24020"
                     /old_locus_tag="JSU13_24005"
     CDS             complement(3886..4689)
                     /locus_tag="JSU13_RS24020"
                     /old_locus_tag="JSU13_24005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000510993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000510993.1"
                     /translation="MGIFSQIGSALLNCISKPVDALCEWAKEPLKDREYRREEASKESE
                     HKRNMDVLTAKKRVEHELRMSELNAEYELREKEKTLEVDLEVRRVREIEKAVAEIQEWR
                     KSQELERMERTTAAIARYKEQLTKLNIEVINSIGNMQLDLKERAQKFVYEKAVQYKRLQ
                     DEATEEAMNDLIRIEEKFGDNERAKDILIRAVDTKMGNVIDTSTRFLEELNRDIVSLNQ
                     SIDRLTNQGQKFIESHLERFHIVDATSSLYITGDEKERLLLKNIN"
                     /name="hypothetical protein"
     gene            complement(4676..5215)
                     /locus_tag="JSU13_RS24025"
                     /old_locus_tag="JSU13_24010"
     CDS             complement(4676..5215)
                     /locus_tag="JSU13_RS24025"
                     /old_locus_tag="JSU13_24010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000897048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000897048.1"
                     /translation="MAGGRHINSLLYVLVAILERKVNDFKLSSSELPEILAALREAREC
                     LVSRSINEDPQLYSALFLYEKNEGLLSLSFEREYSIFINSNLECILRKVSDIKDKKQGK
                     LQKIMNEFVRFKYSSGDSCADLLFLSGKLEQIRKIYEVFDRDRNRLEFLNYMMKKERVI
                     FEREFERLLGEKNGDF"
                     /name="hypothetical protein"
     Typing          complement(2830..2917)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="6.86e-39"
     Typing          2928..3486
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.365"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(626..2175)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="91.985"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 gaatttagca cgttctttac gtaaatcctc ttgcaaagag gaagcaaatt ttatttcttc
       61 cttatattct gcgataagct gcaaataatt cttttcaaac tcatataata cagacaattt
      121 ggtttttaga tgcgcaatgc tgacataatc aactaattct ctttttaaga catttgtcaa
      181 tttttcatta attgaatgat cggcaccaga cgcttcaaca gcccgtgata ttacattata
      241 taaatcactc atatcgttat tcatagaata agtacgtttt cctttggtct caacatcaaa
      301 ctctttcgcc atagaacctc ccaatctctc tatagtgtca cgaaatctga tcatgttttc
      361 taaagccatt ttaatatcgg tcgttttatc tgtcatgact taggcctaac tcatctattt
      421 accataacgt cgcattacac gcaccgctaa aacgcgttca gcacgatcac ggcagcagac
      481 aggtaaaaat ggcaacaaac cacccgaaaa actgccgcga tcgcgcctga taaattttaa
      541 ccgcatgaat acctatgcaa ccagatggta caggccacat taacccctct taatccactt
      601 aacctgccat ttttcatggt ttcaccatcc cagcgaagga ccatccagcg tgcgttcctg
      661 tatttccggc tgacgctccc gttccaggga taatgcatgt tcgcgctcct gtatcagccg
      721 ttcctctctt atctccagtt ctcgctgtat aactggctca agcgttctgt ctgctcgctc
      781 aagtgctgca cctgctgact caactgcatg acccgctcgt tcagcatcgc gttgtcccgt
      841 tgcgtaagcg aaaacatctt ctgcaattcc acgaaggcgc tctcccattc gttcagccgc
      901 tgcatatagt cctgttgcag ttgctctaat gcgttcagca aatgtctttc cagatccgtc
      961 actctgtgtc actccgtcag ttgtacccag tccttcccct gatagggaat caccgttgcc
     1021 gatttcccct gcgggatgac cagaaatttc ctgttcccgt cctgcacaaa ctccacgccc
     1081 catgtcttcg cgttcagttt ctgcaatgtc tcttcctgta tcctgatttc ttccaggttc
     1141 gcctgtatcc gcccgccaag ataccagagc gtcccgccac tcgcggtaaa caggagaaag
     1201 actatcccca gtaacatcac gcccgtattc cctgccagct ttaacacgtc cttccggtgc
     1261 tgcatcatcg cctctttcac cccttcccgg tgttttttca gcgattcctc tgtcgacgtt
     1321 gtgaacaggg ctatagcgtc tctgattttc gtctcgtttg atgtcacagc ctcgcttaca
     1381 gattcgccga gcctcctgaa ctcgttgttc agcattttct ctgtagattc ggctctctct
     1441 ttcagctttt tctcgaactc cgcgcccgtc tgtaaaagat tgctcataaa atgctccttt
     1501 cagcctgata ttcctcccgc cgttcgggtc tgcaatgctg atactgcttc gcgtcaccct
     1561 gaccacttca atccctgcct ccgtgagcgc ctgaatcaca tcctgacggc cttttatctc
     1621 cccgacatga taaagagcgt ctatcccgcg tgtgacgctc tcagcaagcg cctgtttcgt
     1681 ttccggcagg ttatcagaga gagtcagtat ccggcggttc tccggggcgt tggggtcatg
     1741 cagcccgtaa tggtgattca ccagtgtctg ccaggcatca attctcggcc tgtctgcccg
     1801 gtcgtagtac ggctggaggc gttttccggt ctgtagctcc atattcggaa tgacaaaatt
     1861 cagctcaagc cgtcccttgt cctggtgctc cacccacagg atgctgtact gatttttttc
     1921 gagaccgggc atcagtacac gctcaaagct cgccatcact ttttcacgcc ctcccggcgg
     1981 cagctccttc tccgcgaacg acagaacacc tgacgtgtat ttcttcgcaa atggcgtggc
     2041 atcgatgagt tcccggactt cttccggatc accctgaagc accgttgccc cttcccggtt
     2101 acgctccctt cccagcaggt aatcaaccgg accactgcca ccgccttttc ccctggcatg
     2161 aaacttaact atcatcccgc gctccatgtt ccctgacgac ctgccgtaag ctgcgcaact
     2221 ctctctcgat ggccatcagt gcggccacca cctgaacccg gtcaccggaa gaccactgcc
     2281 cgctgttcac cttcctcgcc gtctgattca ggttattccc gatggcagcc agctggcgca
     2341 gtaacggcgg tgccagtgtc ggcagcttcc cggaacgggc aaccggctca cccaggcaga
     2401 tccgccgcat ccataccgcc agctgtttac cctcacagcg ttccagtaac cgtgcatgtt
     2461 catcatcagt aacccgtatc gtgagcatcc tctcgcgttt catcggtatc attaccccat
     2521 gaacagaaat ccccccttac acggaggcat cagtgactaa acaggaaaaa accgccctta
     2581 acatggcccg ctttataaga agccagacat taacgctgct ggagaagctc aacgaactgg
     2641 acgcagatga acaggccgat atttgtgaat cacttcacga ccacgccgat gagctttacc
     2701 gcagctgcct cgcacgtttc ggggatgacg gtgaaaacct ctgacacatg cagctcccgg
     2761 agacggtcac agcttgtctg tgagcggatg ccgggagcag acaagcccgt cagggcgcgt
     2821 cagcaggttt tagcgggtgt cggggcgcag ccctgaccca gtcacgtagc gatagcggag
     2881 tgtatactgg cttaaccatg cggcatcagt gcggattgta tgaaaagtac gccatgccgg
     2941 gtgtgaaatg ccgcacagat gcgtaaggag aaaatgcacg tccaggcgct tttccgcttc
     3001 ctcgctcact gactcgctac gctcggtcgt tcgactgcgg cgagcggtac tgactcacac
     3061 aaaaacggta acacagttat ccacagaatc aggggataag gccggaaaga acatgtgagc
     3121 aaaagaccaa gaacaggaag aaggccacgt agcaggcgtt tttccatagg ctccgccccc
     3181 ctgacgagca tcacaaaaat agacgctcaa gtcagaggtg gcgaaacccg acaggactat
     3241 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
     3301 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct
     3361 cacgctgttg gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
     3421 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
     3481 cggtaagaca cgacaaagcg ccactggcag cagccactgg taactggaca aggtggattg
     3541 agatattaag agttcttgaa gtggtggcct aactgcggct acactagaag gacagtattt
     3601 ggtatctgtg ctccaccaag ccagttacct tcggaagaaa gagttggtag ctctagattc
     3661 cggcaaacaa accaccgttg gtagcggtgg tttttttgtt tacaggcagc agattacgcg
     3721 cagaaaaaaa ggatcacaag aggatccttg aatcttttca ctcccactcc caggtcatag
     3781 ccatagcgct cagttttcaa ccacaagaca aaaactaatg attagcaacc gcaagaacaa
     3841 acaatgcgac cattaaaaat aatgatcgct attactcaca aaatactaat taatattttt
     3901 cagcaacaat cgttctttct catcacctgt aatatataaa gaagatgtcg catcaacaat
     3961 atgaaaacgc tccaaatgac tttcaatgaa tttttgtcct tgattagtca gcctatcaat
     4021 actttggttt aaactaacaa tatctctatt taattcttcc aaaaaccgag ttgacgtatc
     4081 aataacattc cccatcttag tatcaactgc acgaattaaa atatctttag ctctttcatt
     4141 atcaccaaac ttttcctcaa tccttataag atcattcata gcctcttcag ttgcttcatc
     4201 ttgcaggcgc ttatattgta cagctttctc atagacaaat ttctgtgcgc gctcctttaa
     4261 atcaagctgc atgttcccaa tagagttaat cacttctata tttaatttcg ttaattgctc
     4321 cttgtaacgt gcaatagctg ctgttgttcg ctccatcctc tcaagttcct gtgactttct
     4381 ccattcctgg atctcagcta ctgctttttc tatttcgcga acgcgacgaa cctctagatc
     4441 aacttcaaga gttttctctt tttctctcag ctcatattca gcattcaatt cactcattct
     4501 gagttcatgt tcaacacgtt tcttggccgt caagacatcc atgttgcgct tatgttcaga
     4561 ctcctttgaa gcctcctctc ttctatattc cctatctttc aatggttctt tagcccattc
     4621 acagagtgca tcaacaggct tactaataca attaagcaaa gcgctaccaa tctgactaaa
     4681 aatccccatt tttttcacct aaaagccgtt caaactctct ttcaaaaata acacgttctt
     4741 tcttcatcat atagttaaga aattcaagtc tatttctatc cctatcaaag acttcatata
     4801 ttttacgtat ctgttcaagt ttaccactca aaaacaaaag atccgcacaa gaatcgccgc
     4861 ttgaatattt aaacctcaca aattcattca ttattttttg cagtttaccc tgctttttat
     4921 cttttatatc agaaactttc ctcaatatgc actctaaatt ggagttaata aaaatagagt
     4981 attctctctc aaatgaaaga cttagcaacc cttcattttt ttcatataaa aataatgccg
     5041 aatataactg aggatcttca ttgatgctgc gcgatacaag gcattccctt gcctctcgta
     5101 aagcagccaa tatctcagga agttctgacg aagacaattt aaagtcattc actttacgtt
     5161 ctaatatagc cacaagaaca taaagcaaac tatttatatg tctacccccg gccataatta
     5221 gttattgccc ggctcaatag atggtttatt taatttagcc tcagcgcgaa tacaagcaat
     5281 tgtatctaat gtatgctctt caacaaggcc tccgcttaga tccaagcttt tggtataact
     5341 accaacaagt tctttaatcc attttgatgc cacttcatta tcaacttgag attcatttag
     5401 agtttgatga acctctttta atgtctcaga aaa
//
