LOCUS       NZ_CP069671             5514 bp    DNA     circular CON 18-JUN-2023
DEFINITION  Escherichia coli O89m:H9 strain MIN11 plasmid pMUB-MIN11-4, complete
            sequence.
ACCESSION   NZ_CP069671
VERSION     NZ_CP069671.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN17831484
            Assembly: GCF_017570625.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli O89m:H9
  ORGANISM  Escherichia coli O89m:H9
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5514)
  AUTHORS   Majewski,P.
  TITLE     Plasmid mediated mcr-1.1 colistin-resistance in clinical
            extraintestinal Escherichia coli strains isolated in Poland
  JOURNAL   Front Microbiol (2021) In press
REFERENCE   2  (bases 1 to 5514)
  AUTHORS   Majewski,P.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2021) Department of Microbiological Diagnostics
            and Infectious Immunology, Medical University of Bialystok,
            Waszyngtona 15A, Bialystok 15-269, Poland
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: Unicycler hybrid assembly v. v0.4.8-beta
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100x
            Sequencing Technology :: Illumina MiSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 06/18/2023 02:04:53
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,884
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 121
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 13
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 76 of 240
            Pseudo Genes (incomplete)         :: 162 of 240
            Pseudo Genes (internal stop)      :: 55 of 240
            Pseudo Genes (multiple problems)  :: 51 of 240
            Pseudo Genes (short protein)      :: 4 of 240
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP069671.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5514
                     /organism="Escherichia coli O89m:H9"
                     /mol_type="genomic DNA"
                     /strain="MIN11"
                     /serotype="O89m:H9"
                     /isolation_source="lower respiratory tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:2810406"
                     /plasmid="pMUB-MIN11-4"
                     /geo_loc_name="Poland: Bialystok"
                     /lat_lon="53.12523570 N 23.15905989 E"
                     /collection_date="2018-03-29"
                     /genotype="ST-10"
     gene            join(5453..5514,1..574)
                     /gene="kwaA"
                     /locus_tag="JSU13_RS23950"
                     /old_locus_tag="JSU13_23935"
     CDS             join(5453..5514,1..574)
                     /gene="kwaA"
                     /locus_tag="JSU13_RS23950"
                     /old_locus_tag="JSU13_23935"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-phage protein KwaA"
                     /protein_id="WP_058658985.1"
                     /translation="MYLLSMLFLFIPITIYFSGIDNVWSVLDKAMSSCSGADGKFQCVL
                     DYSNSRLTFSVLRSVICGALILTCSYTYIWYRWVLKNLDSGAVSRKLKVVSCKKESGEY
                     LSFLSTYIMPLVFTDLSKPSNIVNFLFVLIIVGFLHIKTKRIHCNPTLSLFNVSAYKIT
                     YSVVANGREKTQDGELIVLSKDLIKENDFIRVINHDDYLTFAKKIIEE"
                     /name="kwaA"
     gene            577..1719
                     /locus_tag="JSU13_RS23955"
                     /old_locus_tag="JSU13_23940"
     CDS             577..1719
                     /locus_tag="JSU13_RS23955"
                     /old_locus_tag="JSU13_23940"
                     /inference="COORDINATES: protein motif:HMM:NF027488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kiwa anti-phage protein KwaB-like
                     domain-containing protein"
                     /protein_id="WP_001704287.1"
                     /translation="MSDDALDLDALEHNEEKAQELLELYKHFDCDINDVTLHTYFNFKN
                     KGKAGAKDSFSYKIAILKNDSELKQSIIDNVIKKTISDLENRVVIVSDVNTLSDRTNTI
                     SFLNDAEQYDNEQMKFLTGIVFDAGFGGDGNENIPIVDFDSVDLISKVDSIIFVLSKNN
                     DDNCITIYKKQYPVQNLSRKKNNFYGFSKKELSRVDQDMISIDGKASLVFAGDTVFIYD
                     QNVFERFFGYKAWIKNAATDFVNNMSTRFKGFVDLSKFIERVNKDSHDADAFSRKLAKA
                     YARSETKQTLQTITNEHLKSVLDENGYLAGVLKYKKGDATICIASHNQQDTFIAMVSEG
                     VLRSLITGIDYLSLGSKRKISSAASNTTTSTTSDSSSAAV"
                     /name="Kiwa anti-phage protein KwaB-like domain-containing
                     protein"
     gene            1946..2272
                     /locus_tag="JSU13_RS23960"
                     /old_locus_tag="JSU13_23945"
     CDS             1946..2272
                     /locus_tag="JSU13_RS23960"
                     /old_locus_tag="JSU13_23945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006674374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_001554615.1"
                     /translation="MEKRTKEIKIRLTEAEHQRLLERCDRKHLAEWLRAVGLGESRTAR
                     RRPLPTVDPTLLRQVSGIGNNLNQIARYLNQHGLPPQERVSLLAVLNSIDQHLAELLEQ
                     HRDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            2430..>2894
                     /locus_tag="JSU13_RS24420"
                     /pseudo=""
     CDS             2430..>2894
                     /locus_tag="JSU13_RS24420"
                     /inference="COORDINATES: protein motif:HMM:NF015397.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            3505..3729
                     /locus_tag="JSU13_RS23970"
                     /old_locus_tag="JSU13_23955"
     CDS             3505..3729
                     /locus_tag="JSU13_RS23970"
                     /old_locus_tag="JSU13_23955"
                     /inference="COORDINATES: protein motif:HMM:NF016769.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_223938297.1"
                     /translation="MEQQLLSALESLQRHYEQQQQAWQDSYANLQRMFEVTSQELAKND
                     RVCQALSMQVTGLAQQVESLNRTVRRLSN"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(3774..4058)
                     /locus_tag="JSU13_RS23975"
                     /old_locus_tag="JSU13_23960"
     CDS             complement(3774..4058)
                     /locus_tag="JSU13_RS23975"
                     /old_locus_tag="JSU13_23960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016149473.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_001554709.1"
                     /translation="MSYNLEFDPRALKEWRKLGDDVRLQFKKKLEQVLQHPRIDKNRLR
                     ELHDCYKIKLRASGYRLVYQVRDQTITVFVVAVGKRERSAAYDAADKRL"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(4048..4290)
                     /locus_tag="JSU13_RS23980"
                     /old_locus_tag="JSU13_23965"
     CDS             complement(4048..4290)
                     /locus_tag="JSU13_RS23980"
                     /old_locus_tag="JSU13_23965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020301039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="WP_001554710.1"
                     /translation="MATINIRIDDELKSRSYAALEKLGVTPSEVLRQTLEYVAQSGRLP
                     FQQVLLTEDDADLMAIVRDRLENPQAGVKVSLDEL"
                     /name="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
     gene            4555..5451
                     /locus_tag="JSU13_RS23985"
                     /old_locus_tag="JSU13_23970"
     CDS             4555..5451
                     /locus_tag="JSU13_RS23985"
                     /old_locus_tag="JSU13_23970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006674370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_024199422.1"
                     /translation="MDNQALTLFNDRLPHKPYFSDDLQFGVRIAGKERALLAKYIQFNQ
                     PHAMYWLCFDVDRAGAAIDWADLGAPAPTLTIKNPENGHAHLLYALHTAVRTAPDGRAA
                     PLKYAAAIENALRKKLGADAGYSGLICKNPNHLHWQITVWQPELYTLDWLADYLDLGAA
                     NDREILPDYGLGRNCTLFDKTRKWAYRAIRQGWPQYDQWLQACIERAKAYNLQFSAPLD
                     ENEVMGIAKSVAKWTSKNLTELGFEEYVKQTHTSEIQRQRGKKSSGGGRPKVRDGEWIS
                     LGISRSTWYRKFYKNEN"
                     /name="replication initiation protein"
     Typing          4418..5451
                     /name="rep_cluster_2486"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          2263..3776
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.406"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 taacgtatgg tctgttttag ataaggccat gtcttcgtgt tctggcgcgg atggaaaatt
       61 tcaatgcgta ctcgactatt caaatagcag attaacattt tctgtattga ggtcagtgat
      121 ctgcggggct ttgattttaa cttgttctta tacatacatt tggtatcgat gggttttgaa
      181 aaacttagac tccggtgcgg tgtcaagaaa gttaaaggtc gtttcatgta aaaaagaatc
      241 cggtgagtat ctttcttttt tatcaacgta cataatgccg ttggtcttta ctgatttaag
      301 caaaccatca aatatagtta attttttgtt tgttttgatt atcgtcggtt ttttgcacat
      361 caaaacaaaa agaattcact gcaacccaac attgtcatta tttaatgttt ctgcctataa
      421 gattacttac agtgtggtag ctaatggaag ggaaaaaacg caagacggtg agttgatagt
      481 tttatctaaa gatttaataa aagaaaatga ttttatcaga gttataaatc atgatgatta
      541 tttaacgttc gcgaaaaaaa tcatagagga atgatgatga gtgacgatgc cttagatctt
      601 gatgctttgg agcataatga agaaaaagca caagagcttt tagaactgta taagcatttt
      661 gattgtgata ttaacgatgt tacattgcac acttacttta actttaaaaa taaaggtaaa
      721 gcgggtgcta aggactcttt tagctataaa attgccatcc ttaaaaatga cagtgagcta
      781 aaacagtcca ttattgataa tgtgataaag aaaactatat ctgatttaga aaatcgggtt
      841 gtgatagtat cagatgttaa tacactaagt gaccgcacca acaccatttc atttttaaat
      901 gatgctgagc agtatgataa tgagcaaatg aagtttctga ctggcattgt gtttgatgct
      961 gggtttggtg gtgatggcaa tgaaaatatc ccaattgttg attttgacag tgtggatcta
     1021 atatcaaaag ttgactcaat cattttcgtt ttgagtaaga ataatgacga caattgtata
     1081 accatataca agaagcaata tccagttcaa aacttatcaa gaaagaaaaa taatttttat
     1141 gggtttagca aaaaagaact atctcgagta gatcaagata tgatttctat tgatggtaag
     1201 gcatctcttg tttttgctgg cgatacggta tttatctacg accaaaatgt tttcgagcgt
     1261 ttttttggct ataaagcatg gataaaaaat gctgccactg attttgttaa caatatgtcc
     1321 actcgtttta agggatttgt tgacttaagc aaatttattg agcgagtaaa taaagatagt
     1381 catgatgcgg atgctttttc tcggaagcta gctaaggcgt atgctcgaag cgaaacaaag
     1441 caaacgctac aaacaataac aaatgaacat cttaagagtg ttctagatga aaatggatat
     1501 ttagcaggtg ttttaaagta taaaaaaggt gatgctacga tttgcatcgc aagccataac
     1561 cagcaagata cgtttatcgc tatggtatcc gaaggcgtat tgagatcgtt gattacaggc
     1621 attgattatt taagccttgg gtcaaagcgg aaaatatcgt cagcagcatc caatactact
     1681 accagtacca cttcggattc ctcttccgct gccgtgtgag acaaaacaac ctatatcaga
     1741 taacagccct gcttttgcgg ggctttttgt ttgtgcgtga tgcgacctcg atgtacgata
     1801 gatgctgtat taagccagta cacacagcgt cccctcttcg aggtgccgtc tgtgactgtt
     1861 tcagggggct cgccgccccc cgaaacccct tgcatccact gcgaaaattc gcactttggg
     1921 tgcgaaactt tctcagcgga ttctcatgga aaagcgcacc aaagagatca aaatcagact
     1981 caccgaagcg gagcatcagc ggctacttga acgctgtgac cgtaagcatt tggccgagtg
     2041 gttacgagcc gttggcttag gcgaatcgcg tacagctcgg cgtcgtccgc tacctaccgt
     2101 agacccgacc ttgttacgtc aggtcagcgg gatcggtaat aacctcaatc aaatagcccg
     2161 ttacttgaat cagcatggct taccgccgca agaacgggtg tcgttgttag cggtgctcaa
     2221 tagcattgac caacatcttg ccgaactgct ggagcaacat cgtgatcgtt aagattcatg
     2281 gtcgtggtgc cggtggcggg agtggtcctg tcgattacct tctgggccct gatcgtcagc
     2341 gcgaacaagc gacggtgtta cggggtaacc ctgagcacgt caaagagctg attgatagct
     2401 gcgaatttgc ccgaacttat acctctggcg tgctctcttt tcaggagagt gatttacccg
     2461 aaggcgaaaa gcagcgattg atggatgaat gggagcagac attgatgacc ggtctagata
     2521 aagaccagta tgcctgcctc tgggttcaac atcaggacaa agggcgtctt gaattgaatt
     2581 ttgttatccc gaacatcgaa ttgcagagcg gaaaacggct acaaccttac tttgatcggg
     2641 ctgaccggcc tcgtgttaac gcatggcaaa ccctcaccaa tgaccggctt gggttacgcg
     2701 acccgaatga ccctgctaat cgccgagcat taaccccctc taatgacctt cctcgcaaca
     2761 aacagcaggc agcggaagcc attaccaaag ggctaatcag cttgattgag cagggagaaa
     2821 ttacggatcg taaaggggtg atttcccacc ttaccgatgc cggattgttg gtcgtacggg
     2881 aaaccaaatc cagtatcagt attgctgatc cggcaggtgg cccgaatatt cgcttaaaag
     2941 gagtgctgta tgagcgagat tttaaattta gcgcgggagt tcgagagcaa atcgaagcag
     3001 caagccaaga ctaccgcaac gagcgtcgcg aacgcattcg agaagcacga gaaacgtatc
     3061 accgaggcct tgaaattaag ctcagggaac atacagaccg ctatccaaga agagaacgac
     3121 agccagctaa aacagataca ccgcttagtc ggaatgacat ggctgtacag cctggcatta
     3181 agtgggatcc tgtttgcgat attgattgga gtagcttggt atctcgggac tatcgtggtc
     3241 gaacgccaga acgaaatcag cgagcagagc cagatcctgc aggacttaaa gagccagacc
     3301 ggagccggcg tatcgataat tcacgattcc aagaacaaga gcgtgtatta cctgatcctt
     3361 ccgcaggggg cgaagaagat cgacgagtac aagaacgctc aacatcgtca ggtcatcaag
     3421 tacagcgcca aataacctca tcagacgcca cagaatcgat tctgggcggt tttatctatc
     3481 agggtgaaga gattcatgac cgaaatggag cagcagcttc tgagcgcatt agagagctta
     3541 cagaggcact acgaacaaca gcagcaagcg tggcaggaca gctacgccaa cttacagcgc
     3601 atgttcgagg ttacctcgca ggagttggcg aaaaacgaca gggtttgtca ggccttgagc
     3661 atgcaagtca ccggcttggc gcagcaagtc gagagcttaa acagaacagt gcgccgcttg
     3721 agcaattagc caagcggcac gaacagcggt attctcgcgg tcacggcatg agtttataag
     3781 cgtttatcgg ccgcatcgta agcggcagaa cgctcgcgct taccgaccgc caccacgaat
     3841 accgtaatgg tttgatcgcg aacctgatag accaagcgat aaccggatgc acggagcttg
     3901 attttgtagc agtcatgcag ctctcgcagg cgatttttat cgatccgcgg gtgttgtaga
     3961 acctgctcga gttttttctt gaactgcaga cggacatcat ccccgagctt gcgccattcc
     4021 ttcagggctc ggggatcaaa ttcaaggtta tagctcatcc agtgacacct ttacgcccgc
     4081 ctgtgggttt tccagacgat cccgaacgat agccatcaaa tcggcatcat cctcggtcag
     4141 caaaacctgc tggaacggca aacgtccgct ttgggccaca tattccagtg tttggcgcag
     4201 aacctcggac ggcgttacgc ccagcttttc cagtgcggca taagagcggc ttttcagctc
     4261 gtcatcgatc cgaatattaa tcgtggccat catctcacct cttgatgtag tgacaagtgt
     4321 atctacaaga agtagtatga gcgtaaagcc gtgcgagaac aagcaggaat aacggattgt
     4381 cggggatgac aaaaaccgtt gttgaggtgt aacttagtgg cagaaaaaac aaagccccga
     4441 aatcatgctc tactttggcg agcggacatg acattcaggg ctaggtcgaa acctagaaag
     4501 gatattagca catgcagcgt gcaaaacaac agccccgcca taaggctggg agccttggat
     4561 aatcaggctt taacgctttt taacgaccgg ttaccccaca agccgtactt ctccgatgat
     4621 ttacagtttg gtgttcgcat tgccggtaaa gagcgtgctc tcctcgcaaa atacatccag
     4681 tttaatcagc ctcacgccat gtactggctt tgctttgacg tggacagagc cggagccgcg
     4741 attgattggg ccgatctggg ggcacctgcg cccacactca ccatcaaaaa ccccgagaac
     4801 ggacatgctc acctgttgta tgccttgcat acggcggtac gtaccgcgcc ggatggtcga
     4861 gctgcccccc ttaaatacgc cgccgccatc gagaatgcgc tgcgtaaaaa attgggcgca
     4921 gatgcggggt attcagggct aatttgcaag aatccgaacc acctgcactg gcagatcacc
     4981 gtctggcagc cagagctcta tacccttgac tggttagccg attacctcga ccttggcgca
     5041 gccaatgacc gcgaaatcct gccggactac ggtttaggcc gtaactgcac cctcttcgat
     5101 aaaacccgca agtgggctta ccgcgctatc cgccaaggct ggccgcagta tgaccaatgg
     5161 ctacaagcct gcattgaacg cgctaaagcc tacaacctgc agttctccgc acctttagac
     5221 gagaacgaag tcatgggaat tgctaaaagt gtggctaagt ggacaagtaa gaaccttaca
     5281 gagcttggct ttgaagagta tgttaaacaa acacatactt ctgaaattca gcgtcaacgt
     5341 ggaaaaaaaa gctctggtgg tggaaggccg aaagttagag atggagaatg gatttcttta
     5401 ggaataagta gatcaacgtg gtacaggaaa ttttacaaaa atgaaaatta gaatgtatct
     5461 cttatcaatg ttgtttttat ttataccgat aacaatatat ttttctggta ttga
//
