LOCUS       NZ_CP069670             5874 bp    DNA     circular CON 18-JUN-2023
DEFINITION  Escherichia coli O89m:H9 strain MIN11 plasmid pMUB-MIN11-3, complete
            sequence.
ACCESSION   NZ_CP069670
VERSION     NZ_CP069670.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN17831484
            Assembly: GCF_017570625.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli O89m:H9
  ORGANISM  Escherichia coli O89m:H9
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5874)
  AUTHORS   Majewski,P.
  TITLE     Plasmid mediated mcr-1.1 colistin-resistance in clinical
            extraintestinal Escherichia coli strains isolated in Poland
  JOURNAL   Front Microbiol (2021) In press
REFERENCE   2  (bases 1 to 5874)
  AUTHORS   Majewski,P.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2021) Department of Microbiological Diagnostics
            and Infectious Immunology, Medical University of Bialystok,
            Waszyngtona 15A, Bialystok 15-269, Poland
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: Unicycler hybrid assembly v. v0.4.8-beta
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100x
            Sequencing Technology :: Illumina MiSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 06/18/2023 02:04:53
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,884
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 121
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 13
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 76 of 240
            Pseudo Genes (incomplete)         :: 162 of 240
            Pseudo Genes (internal stop)      :: 55 of 240
            Pseudo Genes (multiple problems)  :: 51 of 240
            Pseudo Genes (short protein)      :: 4 of 240
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP069670.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5874
                     /organism="Escherichia coli O89m:H9"
                     /mol_type="genomic DNA"
                     /strain="MIN11"
                     /serotype="O89m:H9"
                     /isolation_source="lower respiratory tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:2810406"
                     /plasmid="pMUB-MIN11-3"
                     /geo_loc_name="Poland: Bialystok"
                     /lat_lon="53.12523570 N 23.15905989 E"
                     /collection_date="2018-03-29"
                     /genotype="ST-10"
     gene            complement(join(5440..5874,1..618))
                     /locus_tag="JSU13_RS23915"
                     /old_locus_tag="JSU13_23900"
     CDS             complement(join(5440..5874,1..618))
                     /locus_tag="JSU13_RS23915"
                     /old_locus_tag="JSU13_23900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001557502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_021517772.1"
                     /translation="MNLQQRINKLPQLSSSFSFGKDIDNIHSFIFNETSKDKIEDLLRK
                     WVSGNQPCVFGKLASKKIKGLDFHLSIVNSPQLYNDDGHLFDFLRNERVRFKERARRGE
                     VSAHLIYFIHPQLAFARPSEELVDIQKYICSLHMPECYPIKEDVIYTESVPFQDKDGLK
                     IYKAGVNVFYSSAHRTRNHDRRIPGGILISVNAPGHFMRLAIEKGFYKDQEQALADIRN
                     MTIQSVGNGGYSHPEGISTTWHSESKLDRFGCPVHTGNSSYYSGFYHTDVLIPGELTKD
                     ERLLHEIDNSDPMIFNWNVLFYVSLEEFPIDDPYYGEFIGVPVDDASMFFNSFQPRKFE
                     NNPLYEKEDD"
                     /name="hypothetical protein"
     gene            complement(1338..1571)
                     /locus_tag="JSU13_RS23920"
                     /old_locus_tag="JSU13_23905"
     CDS             complement(1338..1571)
                     /locus_tag="JSU13_RS23920"
                     /old_locus_tag="JSU13_23905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_021520366.1"
                     /translation="MTELERHLLNALEQLQQDYMQRLNEWENAFVELQKMFSLTQRDNA
                     MLNGRVMQLSQQVQHLSERTERLSQLYSENWR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(1578..2108)
                     /locus_tag="JSU13_RS23925"
                     /old_locus_tag="JSU13_23910"
     CDS             complement(1578..2108)
                     /locus_tag="JSU13_RS23925"
                     /old_locus_tag="JSU13_23910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001673158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_001673158.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTEKMLNDEFRKLEESVNRELTSNE
                     SLIRNAINDHTTALKELLERYQKTTVDTMDAHWKTVLKMSVKRWLWLIIVSVLMFATTG
                     SLLWYQGMKINANMNILREQKESLEKLNAKTWGVRYHEDSNGRFLVLPKGMKAETNWTK
                     DNGKLNAVRLVQE"
                     /name="MbeB family mobilization protein"
     gene            complement(<2146..2577)
                     /locus_tag="JSU13_RS24415"
                     /pseudo=""
     CDS             complement(<2146..2577)
                     /locus_tag="JSU13_RS24415"
                     /inference="COORDINATES: protein motif:HMM:NF015397.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(2786..3109)
                     /locus_tag="JSU13_RS23935"
                     /old_locus_tag="JSU13_23920"
     CDS             complement(2786..3109)
                     /locus_tag="JSU13_RS23935"
                     /old_locus_tag="JSU13_23920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_024188420.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAAWMRRVCLGEPVARSGKLPTL
                     APPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVVAALIAIGDELRRLRLAVREQGAR
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            3175..3366
                     /locus_tag="JSU13_RS23940"
                     /old_locus_tag="JSU13_23925"
     CDS             3175..3366
                     /locus_tag="JSU13_RS23940"
                     /old_locus_tag="JSU13_23925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_021539356.1"
                     /translation="MTKQEKTALNMVRFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENR"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(4572..5438)
                     /locus_tag="JSU13_RS23945"
                     /old_locus_tag="JSU13_23930"
     CDS             complement(4572..5438)
                     /locus_tag="JSU13_RS23945"
                     /old_locus_tag="JSU13_23930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001335763.1"
                     /GO_function="GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="WP_001335763.1"
                     /translation="MIKGMLFAFLAAFSWGAAIVMSKKGVEELDAGGVFFWQICSAVIL
                     SWIVLFIKRKKLPLNSKAILAYATGVFEPFLAYTFTLYGLYFISAGSASIIFSLESVFI
                     LVLSVLICSVKINSPQYFILFLIGAIVGSILVALPDISNGSNNIVGYCLVVAGVLSAAF
                     YVVISSRLIKSFEPITLLSGQLTFSLILSSLFIFISKSSIYLPLESVIIVFSSGILQYF
                     LAFCFYLNSLRWLPVHLAGIMLYFIPLVALFLSWLFLDEQFTFLQGVGFIVTVLSVYFL
                     NRKYSEE"
                     /name="DMT family transporter"
     Typing          complement(3461..3542)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="98.795"
                     /coverage="100.0"
                     /evalue="6.23e-34"
     Typing          complement(3999..4186)
                     /name="rep_cluster_2401"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="4.63e-94"
     Typing          complement(1235..2796)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.232"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 ttctatcgcc agcctcataa aatgccccgg ggcatttaca gaaataagta ttccccccgg
       61 tattcttcta tcatgatttc ttgttctatg agcagaagag taaaaaacat taacaccagc
      121 cttatatatt tttagcccat ctttatcctg aaatggtacg ctttcagtat aaataacatc
      181 ctcttttatt ggatagcact ctggcatatg cagcgaacaa atatattttt gtatgtccac
      241 taactcttct gatggacgag caaatgcaag ttgtggatga ataaaataaa tgagatgcgc
      301 actgacctct cctcttcttg cgcgttcttt aaaacgaact ctttcattac gcaaaaaatc
      361 aaacaaatga ccatcatcat tatatagttg ggggctattt acaatagata aatggaaatc
      421 aagcccttta attttcttgc ttgccaattt accaaataca cagggctgat tgcctgacac
      481 ccattttctt agtaaatctt caattttatc tttagaagtt tcgttaaaga taaatgaatg
      541 aatattatca atatcttttc caaatgaaaa actggatgat aattgtggta gtttatttat
      601 acgttgttga agattcatga aagctcccac aagacacctc atatattttt tatatactga
      661 aactacatta tatttattac cgacgtaaaa cgaaataatt tattatcgtt accgatattt
      721 taaaaaatca ttatttgcac agtgttcgag ttgttagtat cactccttcc tgaaaatccg
      781 ctccatttac cataacgtcg cattgtacgc accgccaaaa cgcgttcggt gcgatcacgg
      841 cagcagacag gtaaaaatgg caacaaacca cccgaaaaac tgccgcgatc gcacccgata
      901 aattttaacc acatgcatag ctatgcagcc atgtgaatca cgcaggaatt gccagccaga
      961 gactgctgaa acggattttt ttcatacttt cggaaaggca gaagacggaa ccggagcgga
     1021 aaaacagaaa ttcatgggaa tgagcgcgat ctggcaataa cggcaaaaca cagcaacaaa
     1081 gacacaccag aatcgcgccc gcatgcgttt taacgcgttc ctgtgttttc agcaagctct
     1141 gactgaagcg cgttcccggt gcccccgaac caaccattat cccaaaaaat gcaccggaaa
     1201 cgcgctgcca acattttagc ctgtctctgg ttaccatccc agcgaagggc catccagtgt
     1261 ccgttcctga atttccggct ggcgctcccg ctccagggat aacgcatatt cgcgctcctg
     1321 tatcagccgc tcatccctta tctccagttc tcgctgtata actggctcaa gcgttctgtc
     1381 cgctcgctca agtgctgcac ctgctgactc aactgcatga ctcgcccgtt cagcatcgcg
     1441 ttgtcccgtt gcgtaagcga aaacattttc tgcaattcca cgaaggcgtt ctcccattcg
     1501 ttcagccgct gcatatagtc ctgttgcagt tgctctaatg cgttcagcaa atgtctttcc
     1561 agctccgtca tgctctgtca ctcctgcacc agtctcactg cgttcagttt gccgttgtcc
     1621 ttcgtccagt tcgtttcggc tttcatcccc ttcggcaaca ccagaaaacg tccgttgctg
     1681 tcctcgtgat aacgcacgcc ccatgtcttc gcgttcagct tctccagact ctctttctgc
     1741 tcgcggagaa tattcatatt cgcgtttatc ttcatgccct ggtaccacag cagactcccg
     1801 gtcgtggcaa acatcagtac tgagacgata atcagccaca gccagcgttt cacgctcatt
     1861 ttcagtacgg ttttccagtg cgcgtccatc gtatcgacgg tcgttttctg atacctctcc
     1921 agcagttctt tcagggctgt tgtatggtcg ttgatagcgt tcctgattaa agattcgttt
     1981 gaggtcagtt ccctgtttac agattcttcg agcttcctga actcgtcgtt cagcattttc
     2041 tctgtagatt cggctctctc tttcagcttt ttctcgaact ccgcgcccgt ctgtaaaaga
     2101 ttgctcataa aacgctcctt tcagtctgat attcctcccg ccgttcgggt ctgcaatgct
     2161 gatactgctt cgcgtcaccc tgaccacttc aagccccgcc tctgtgagcg cctgaatcac
     2221 atcctgacgg ccttttatct ctccggcatg gtaaagcgcg tctatcccgc gtgtgacgcc
     2281 ctcagcaagc gcctgtttcg tttccggcag gttatcagga agggtcagcg tccggcggtt
     2341 ctccggggcg ttggggtcat gcagaccgta atggtgattc accagcgtct gccaggcatc
     2401 aattctcggc ctgtctgccc ggtcgtagta cggctggagg cgttttccgg tctgtagctc
     2461 catgttcgga atgacaaaat tcagctcaag ccgtcccttg tcctggtgct ccacccacag
     2521 gatgctgtac tgatttttct cgagaccggg catcagtaca cgctcaaagc tcgccatcac
     2581 tttttcacgc cctccaggcg gcagctcctt ctccgcgaac gacagaacac ctgacgtgta
     2641 tttcttcgca aatggcgtgg catcgatgag ttcccggacc tcttccggat tcccctgaag
     2701 caccgttgcc ccttcccggt tacgctccct tcccagcagg taatcaaccg gaccactgcc
     2761 accacctttt cccctggcat gaaacttaac tatcatcccg cgccccctgt tccctgaccg
     2821 ccagacgcag ccggcgcagc tcatccccga tggctatcag tgcggccacc acctgaaccc
     2881 ggtcaccgga agaccactgc ccgctgttca ccttcctcgc tgtctggtta aggttattcc
     2941 cgatagcggc cagctgacgc agtaacggcg gtgccagtgt cggcagcttc ccggaacggg
     3001 caacaggctc acccaggcag acccgccgca tccatgccgc cagttgttta ccctcacagc
     3061 gttccagtaa acgggcatgc tcatcatcag tcacccgtat cgtgagcatc ctctcgcgtt
     3121 tcatcggtat cattaccccc atgaacagaa atccccctta cacggaggca tccagtgact
     3181 aaacaggaaa aaaccgccct taacatggtc cgctttatca gaagccagac attaacgctg
     3241 ctggagaagc tcaacgaact ggacgcggat gaacaggcag acatctgtga atcgcttcac
     3301 gaccacgccg atgagcttta ccgcagttgc ctcgcacgct tcggggatga cggtgaaaac
     3361 cgctgacaca tgcagctccc ggagacggtc acagcttgtc tgtgagcgga tgccgggagc
     3421 agacaagccc gccagggcgc gtcaggccgt tgacctttgg ggtgtcgggg cgaagccctg
     3481 acccagtcac gtagcgatag cggagtgtat actggcttaa tcattcggca tcagtgcgga
     3541 ttgtatgaaa agtgcgccat gtcgggtgtg aaatgccgca cagatgcgta aggagaaaat
     3601 gccagttcag gcgctcttcc gcttcctcgc tcactgactc gctacgctcg gtcgttcggc
     3661 tgcggcgagc ggactctcct ttctcaaaaa cgctaaccct gttatccaca gaacccgggg
     3721 ataaaccagg aaagaacatg tgcgcaaaaa acgaaacccc ggaaaaggcc gcgccggagg
     3781 cgctttttcc ataggctccg cccccctgac ggacttcaca aaacagacac tttttcggtg
     3841 catctgtggg agccttgcgg ctcaaccatg aaatccgcca gcggtggcga aacccgacgt
     3901 ggcttaaaga tcatcaccgt ttccggtgaa tcgctccctc ctgcgccccc acgttcccgc
     3961 catatcgatt accggatacc ttttccgcct ttctccctta cgggacacgt ggcgctttct
     4021 catagctcac gtcttctggt atctcggggc gacttaggca gttcgctccg ggctgggctg
     4081 tatgcaggaa ccccccgttc agcccgactg ctgcgccttc tccggtaact gttctcttgg
     4141 gtccaacccg gaaaagacac cacaaagcgc cactggcagc agccattggt aaaagtcatg
     4201 tgcgagatat ttagagagta cttgaagtgg ctgccggagt gcggctacac tagaagaaca
     4261 attttggtga ctcgtctcgc ataagacagt tacctggtta agcagtctgc aagctgactt
     4321 aaccttcgat caaaccgccc tgcaaggcgg ttttttcgtt tacaggcagc agattacgcg
     4381 cagaaaaaaa ggatctcaag aggatccttt gatctttttg ttacaaacgt ctctatttga
     4441 ctcccctgag agcgttttag cgcgcctctg ccccccttgg ttacattccg acaccagaaa
     4501 cgctcccgtt ttttacgtgc atttggcacg gtatcgatgg aggccggggg ggggcaatcc
     4561 tgaacagaat gttactcctc tgagtatttt ctgttaagaa aatatacact tagcacagtt
     4621 acaataaatc ctaccccctg taagaatgtg aattgttcat ccaaaaacaa ccatgacaaa
     4681 aataatgcaa ctagcggaat aaagtacaac attatccctg ccaaatgaac tgggagccaa
     4741 cgcaaggagt tcaagtaaaa acaaaaagct aaaaaatatt gtaatattcc agatgaaaaa
     4801 acaataatca cggattccaa cggaagataa attgaggatt tagatataaa aataaagagc
     4861 gatgacagta taagggagaa tgtcaactgt ccgctcaata atgtgatagg ctcaaacgat
     4921 ttaataaggc gagacgatat aacaacataa aatgctgccg ataacacacc ggcaacaaca
     4981 agacaatatc caacaatatt attcgatcca ttactgatgt ctggcaaggc taccaaaata
     5041 ctgccaacaa ttgcacctat aaggaacaat ataaaatatt gcggggaatt aattttaact
     5101 gagcaaataa gaacagacaa caccaatata aacactgatt caagtgaaaa aataattgat
     5161 gcgcttcctg ctgaaataaa ataaagtccg tagagtgtaa aagtatacgc taagaatggt
     5221 tcaaaaacgc ctgtcgcata agcaagtatc gcttttgagt ttaatgggag tttttttctt
     5281 tttataaaaa gaactatcca agatagtatt actgcactac aaatctgcca gaaaaacacc
     5341 cctccggcat ctagttcttc aactcctttt ttgctcatta cgatagctgc cccccatgag
     5401 aacgctgcta aaaaagcaaa aagcattcct ttaatcatat taatcatcct ctttctcata
     5461 aagggggttg ttttcaaatt tccttggttg aaaggagtta aaaaacatag atgcatcatc
     5521 tacaggaaca ccaataaact ctccataata aggatcatct ataggaaact cttctaatga
     5581 aacataaaac aatacattcc agttgaagat cattggatca ctattatcta tttcatggag
     5641 caatctttca tccttagtta attctcctgg aattaataca tcagtatgat aaaaaccgct
     5701 gtaataagaa ctattaccgg tgtgcactgg gcatccaaaa cgatccaatt tagattctga
     5761 atgccatgtt gtagaaatcc cttccggatg tgaataccca ccgttaccaa cagactgaat
     5821 agtcatgtta cgaatatccg ctaacgcttg ttcttgatct ttatagaaac cttt
//
