LOCUS       NZ_CP069665             2255 bp    DNA     circular CON 18-JUN-2023
DEFINITION  Escherichia coli O89m:H10 strain MIN12 plasmid pMUB-MIN12-7,
            complete sequence.
ACCESSION   NZ_CP069665
VERSION     NZ_CP069665.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN17831485
            Assembly: GCF_017570605.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli O89m:H10
  ORGANISM  Escherichia coli O89m:H10
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2255)
  AUTHORS   Majewski,P.
  TITLE     Plasmid mediated mcr-1.1 colistin-resistance in clinical
            extraintestinal Escherichia coli strains isolated in Poland
  JOURNAL   Front Microbiol (2021) In press
REFERENCE   2  (bases 1 to 2255)
  AUTHORS   Majewski,P.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2021) Department of Microbiological Diagnostics
            and Infectious Immunology, Medical University of Bialystok,
            Waszyngtona 15A, Bialystok 15-269, Poland
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: Unicycler hybrid assembly v. v0.4.8-beta
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 484x
            Sequencing Technology :: Illumina MiSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 06/18/2023 02:09:43
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,209
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,805
            CDSs (with protein)               :: 4,805
            Genes (RNA)                       :: 122
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 14
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 89 of 282
            Pseudo Genes (incomplete)         :: 204 of 282
            Pseudo Genes (internal stop)      :: 44 of 282
            Pseudo Genes (multiple problems)  :: 55 of 282
            Pseudo Genes (short protein)      :: 4 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP069665.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2255
                     /organism="Escherichia coli O89m:H10"
                     /mol_type="genomic DNA"
                     /strain="MIN12"
                     /serotype="O89m:H10"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:2810405"
                     /plasmid="pMUB-MIN12-7"
                     /geo_loc_name="Poland: Bialystok"
                     /lat_lon="53.12523570 N 23.15905989 E"
                     /collection_date="2018-05-05"
                     /genotype="ST-10"
     gene            139..654
                     /locus_tag="JSU14_RS25650"
                     /old_locus_tag="JSU14_25690"
     CDS             139..654
                     /locus_tag="JSU14_RS25650"
                     /old_locus_tag="JSU14_25690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000097059.1"
                     /GO_process="GO:0006260 - DNA replication [Evidence IEA];
                     GO:0006276 - plasmid maintenance [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication/maintenance protein RepL"
                     /protein_id="WP_000097059.1"
                     /translation="MSSLSLQRYETNPFLELMTIPMKGKRIQVSRLGRDDNVLVNQNTG
                     EKFGTHVTTFKRVDSEQFVKLFTANIGLTFDLSSAGIKAFNVLLWAVQKQAFAKDQVLL
                     DSRALDDFLKSYDSLKLSYATLKRGINELEKAQIIAKTMRKGFYFINPNFVFNGDRIAF
                     TTLIERDC"
                     /name="replication/maintenance protein RepL"
     gene            729..1013
                     /locus_tag="JSU14_RS25655"
                     /old_locus_tag="JSU14_25695"
     CDS             729..1013
                     /locus_tag="JSU14_RS25655"
                     /old_locus_tag="JSU14_25695"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000160398.1"
                     /translation="MTKHDAIRISQLHQIFYDDEGLIDSEKNVTILYSIGFTIDEIAYF
                     GNTTPGTVQGQLFNARAKLSCASASSLRPMILLRLLSLIIFEQDRLNRL"
                     /name="hypothetical protein"
     gene            1116..1619
                     /gene="ddp1"
                     /locus_tag="JSU14_RS25660"
                     /old_locus_tag="JSU14_25700"
     CDS             1116..1619
                     /gene="ddp1"
                     /locus_tag="JSU14_RS25660"
                     /old_locus_tag="JSU14_25700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000809479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA distortion polypeptide 1"
                     /protein_id="WP_000809479.1"
                     /translation="MKQIKFRLTDEEYNVAHINAISNGVNSVNAFAKRKVLDISATPSK
                     VIVSNEPSVPVKTCLYPHELELIKRNAALHGMSVSREIAIRLRKSVLKSEVCLYPEEVQ
                     VLRKLSTTINRIGRNIHFIISGDRFCTVNDPDFRNEIRELTALCETVDKTIEQLVFSVN
                     NRFG"
                     /name="ddp1"
     Typing          complement(1865..2125)
                     /name="Col(MG828)"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.511"
                     /coverage="100.0"
                     /evalue="3.95e-106"
ORIGIN
        1 gcatgaatcc tagaaagtgg tgatcaggat ctgtaccttg taagctcaga tccggtacct
       61 tgcaggctca aaaaataatt gattttgagc ttatgaggat ttattgtgaa tctccaaagc
      121 ccaaaaaagg atgcaataat gagctcacta agcttgcaga gatacgaaac gaacccattt
      181 ttagagctta tgacaatccc gatgaagggg aaacggatac aagtatctcg tcttggacgt
      241 gatgacaacg ttttggttaa tcaaaacacg ggagaaaagt ttgggacgca tgtaaccact
      301 tttaagcgtg tggatagtga gcaatttgtt aaattgttta ctgcgaatat tgggctaaca
      361 tttgatttat catcggcagg aattaaagct tttaatgtcc tgttatgggc tgttcaaaaa
      421 caggcttttg caaaagatca ggttctttta gattccagag ctcttgatga ctttttgaaa
      481 agctatgata gcttaaaact aagttacgct acgttaaagc gagggatcaa cgagttagaa
      541 aaagctcaga ttatagcaaa gacaatgcga aaaggatttt atttcataaa tcctaacttt
      601 gtatttaatg gcgatagaat agcgtttaca actctgatcg agagggattg ttaatataat
      661 cgtcgcagtc gaacaaaact ccacttttgt tcgactgcta atcacaacaa cctattaggg
      721 aggttattat gactaagcat gatgctatac gcatttctca acttcatcaa atcttttacg
      781 atgacgaggg attaattgat tcagaaaaga acgtgaccat tttatacagc ataggattta
      841 ctattgatga aatagcttat tttgggaata caacaccggg aacagttcag ggacagttat
      901 ttaatgcaag agctaaatta agttgtgctt cagcttcatc tttaagaccg atgattttgt
      961 tgagattgct ttcactcata atctttgagc aagaccgctt aaataggctg taatcaatgc
     1021 aggatagaca atcatggtca aatgggtacc attttccatg attgtccaaa aacggggttt
     1081 ttgcctgtgc ctgccgcagg ctcttaggag gttctatgaa gcaaattaaa ttcagattga
     1141 ccgacgagga atacaacgtt gctcatatta atgctatttc aaatggcgtt aatagtgtaa
     1201 atgcttttgc aaagaggaag gttttagata ttagcgcaac accttcaaag gttatcgtat
     1261 cgaatgaacc atctgtacca gtaaaaacat gtttataccc acatgaactg gagttaataa
     1321 agcgtaatgc tgcattacac gggatgagcg ttagccgaga aatagcaata cgcttacgta
     1381 aatcagtgtt gaaaagcgaa gtttgtcttt atcccgaaga agtgcaggtg ctcagaaagc
     1441 tttcaacaac tattaataga attggtcgaa atattcattt tataattagt ggcgatcgtt
     1501 tttgcacagt taacgatcct gatttcagaa atgagataag ggaacttaca gcattatgcg
     1561 aaacagtcga taaaacaata gagcaattag tttttagtgt taataatcgt tttgggtaaa
     1621 aacgtattat caagaaccga ctgtaggtca tgggcaaacg ttgcagaatt gcgtcagaga
     1681 cgtcattttg cggcgtttgc cctatcctgc atcggggtta aaccacgaag atttttattc
     1741 cccgttcttc ggaacgaccg aagccgccac ggttccgatt gcgccttgag cgcaaccgga
     1801 aaccgactgg ggagaacccc agacccccat cgcggcaaca ccgacggtct acgaccacct
     1861 accaaccgcg tggctgcctg ttccgtgcaa atgggctttg cagggggctg acgcggttcc
     1921 tggcgacctg aaagcccctg gttaggggta tcggcgcgcc tgacctcctg aaaccctctg
     1981 taagcgtttt tcgcgccttc catgccctga cgggcattta aaccccgtct cgtgtgtctg
     2041 agagcttctg agcgcgtttc aggggctatc tgacgactca tccccgaaag aataacaacg
     2101 ggatcaccga caacatgcga gaggtgacac cgctcgccgc agccccagcg accgaacgta
     2161 gtgagcgagt gggcgaggaa gcggaagaga accagaagcc atattgagca cttacgctcg
     2221 tagttaagaa ctaagaaggg aatttcttga aaccc
//
