LOCUS       NZ_CP069664             3897 bp    DNA     circular CON 18-JUN-2023
DEFINITION  Escherichia coli O89m:H10 strain MIN12 plasmid pMUB-MIN12-6,
            complete sequence.
ACCESSION   NZ_CP069664
VERSION     NZ_CP069664.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN17831485
            Assembly: GCF_017570605.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli O89m:H10
  ORGANISM  Escherichia coli O89m:H10
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3897)
  AUTHORS   Majewski,P.
  TITLE     Plasmid mediated mcr-1.1 colistin-resistance in clinical
            extraintestinal Escherichia coli strains isolated in Poland
  JOURNAL   Front Microbiol (2021) In press
REFERENCE   2  (bases 1 to 3897)
  AUTHORS   Majewski,P.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2021) Department of Microbiological Diagnostics
            and Infectious Immunology, Medical University of Bialystok,
            Waszyngtona 15A, Bialystok 15-269, Poland
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: Unicycler hybrid assembly v. v0.4.8-beta
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 484x
            Sequencing Technology :: Illumina MiSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 06/18/2023 02:09:43
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,209
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,805
            CDSs (with protein)               :: 4,805
            Genes (RNA)                       :: 122
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 14
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 89 of 282
            Pseudo Genes (incomplete)         :: 204 of 282
            Pseudo Genes (internal stop)      :: 44 of 282
            Pseudo Genes (multiple problems)  :: 55 of 282
            Pseudo Genes (short protein)      :: 4 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP069664.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3897
                     /organism="Escherichia coli O89m:H10"
                     /mol_type="genomic DNA"
                     /strain="MIN12"
                     /serotype="O89m:H10"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:2810405"
                     /plasmid="pMUB-MIN12-6"
                     /geo_loc_name="Poland: Bialystok"
                     /lat_lon="53.12523570 N 23.15905989 E"
                     /collection_date="2018-05-05"
                     /genotype="ST-10"
     gene            complement(53..448)
                     /locus_tag="JSU14_RS25630"
                     /old_locus_tag="JSU14_25670"
     CDS             complement(53..448)
                     /locus_tag="JSU14_RS25630"
                     /old_locus_tag="JSU14_25670"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001222910.1"
                     /translation="MRISNIDWLKKRIGFIRKLGEQTARQRQIIDLLDNEAGLTEQERK
                     LLHVLATAEKNELQAQENARKQANQKRMEGKTQRRERNHRLFLAAGLLIEAGLVDTKTG
                     ELRYPKDNILQKLKAIRLDLETSPDHH"
                     /name="hypothetical protein"
     gene            628..1581
                     /locus_tag="JSU14_RS25635"
                     /old_locus_tag="JSU14_25675"
     CDS             628..1581
                     /locus_tag="JSU14_RS25635"
                     /old_locus_tag="JSU14_25675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="WP_001360358.1"
                     /translation="MATYHLNVKFGGKGQAGNHADYIERKGEYEKRKDLEYSEHGNMPG
                     WARDNPSHFWQAADQFERANGSTYREIEIALPRELTPDQRIELVQDFVRQVAGERHAYS
                     FAIHNPKASIDGGEQPHAHIMMSQRINDGMERSPEQYFRRYNAKNPERGGAKKDSGNLT
                     PTQQKEQLRTLRASWSALQNEHLARHGHRVRVDHRSLEERGIKRTPERHFGFAASSRLD
                     HAQRREILQNRAQPLEHFLEKAHSVRHQFDAQVQQKELVKTLLQDVARERGHALTARIN
                     KTGEVEFERAPTRTRSNTRGHGMGIRDNSRGYDYER"
                     /name="MobA/MobL family protein"
     gene            1593..2123
                     /locus_tag="JSU14_RS25640"
                     /old_locus_tag="JSU14_25680"
     CDS             1593..2123
                     /locus_tag="JSU14_RS25640"
                     /old_locus_tag="JSU14_25680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000168768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000168766.1"
                     /translation="MASTEEGLLTLLLSVEHLKNTAIEQQKAVADTLAVARETLRGYRD
                     ISDSVVKQVSDEVRKGVQKTDIAGLMGERMTQDFQRLEETVAKMRANAEALNKDIATIH
                     HNVVKEYNTLNKYSLKWFIGVTVSFLVMFSLLGWFTKTLIDRNYTATEVVYQKIVDIEK
                     KLDKPATGKSRKN"
                     /name="hypothetical protein"
     gene            2556..3587
                     /locus_tag="JSU14_RS25645"
                     /old_locus_tag="JSU14_25685"
     CDS             2556..3587
                     /locus_tag="JSU14_RS25645"
                     /old_locus_tag="JSU14_25685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001271372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_047611220.1"
                     /translation="MSAALQYFEENLPHRPYHTDDLAFGLRISGKGRALLARYIQQNQP
                     HAQFWLVFDVDREGAAIDWSDRNAPAPNITVKNPVNGHAHLLYALNIAVRTAPDASVKA
                     LKYAAAVERSLCEKLCADVNYSGLICKNPFHLEWLVMEWREEAYTLDELADYLDLSASA
                     RRSIDKHYGMGRNCHLFEMTRKWAYRAIRQGWPAFSQWLDAVIQRVEMYNASLPVPLSP
                     AECRAIGKSIAKYTHRNFTPETFAQYVADTHTPEIQAARGRKGGKANSSKNQADKGKVG
                     GKNSGVVRWTANDDKRRRALDMYILGASTEDIAVAVGVSRWTVRRWMDSSVEWLLSKQI
                     IKI"
                     /name="replication initiation protein"
     Typing          2557..3332
                     /name="Col156"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="97.683"
                     /coverage="83.0"
                     /evalue="0.0"
     Typing          629..1578
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.685"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 gaaatcgaaa acattgaaca ccggcaggca aacgcaccac cacgcagcgg aattaatgat
       61 gatctgggct tgtttcaaga tcgagcctga ttgccttaag tttctgtaat atattgtctt
      121 ttgggtatct cagttcgcct gtttttgtgt ccactagtcc ggcctcgatc agtaaccccg
      181 ccgcaagaaa caggcggtga ttgcgttccc gtcgttgtgt tttcccctcc atccgttttt
      241 ggttggcctg tttgcgagcg ttctcctgtg cctgtagctc gtttttttct gcggttgcca
      301 ggacgtggag tagtttgcgc tcctgttctg ttagtccggc ttcgttatcg agcaggtcaa
      361 tgatctgcct ctgtcgtgcg gtctgttctc cgagcttcct gataaagccg attcgtttct
      421 tcaaccagtc gatgttgctg atgcgcatgt ttgggatgct ccctggtgtt ttttgcggtg
      481 tgttggggtt gtgcagtata tcgcttgcgg tgggtgggtg caggggggta tcttccgtgc
      541 taattcgtaa aattagcagg aagcgcactt atatgtttct tcgccgttgg ctcagaaaca
      601 tgtgcgctcc tgcggagggc taacaccgtg gcaacctatc acctcaatgt aaaatttggc
      661 ggcaagggac aggccggaaa ccatgccgat tacatcgaac gcaaaggcga gtacgagaaa
      721 cgaaaagatc tggaatactc agagcacggg aatatgccag gctgggcgcg agataatccc
      781 tcccatttct ggcaagcggc tgatcagttt gaacgtgcga atggttcgac ttatcgggag
      841 atcgaaatcg ccttgccgcg tgaactgaca ccggatcagc gcattgaact ggtgcaggat
      901 tttgtgcgtc aggtagctgg agaacggcac gcctatagct ttgccattca caatccaaaa
      961 gcgagcattg acggagggga acagcctcat gcgcatatca tgatgagcca gcgtattaac
     1021 gatggtatgg aacgttcgcc ggaacagtat ttccggcgtt ataacgccaa gaatcccgaa
     1081 cggggaggcg caaaaaaaga ttcaggcaat ctgacaccga ctcagcaaaa agagcaattg
     1141 cggacgttac gggcgagctg gtcagcgtta cagaacgagc atttagcgcg tcatggtcac
     1201 agggtgcgcg ttgaccatcg ctcactggaa gaacgcggaa ttaaacgcac gccagaacgg
     1261 catttcggat ttgccgcctc atcacggctt gatcatgcgc agcgccgcga aatcctgcaa
     1321 aaccgagcgc agccgctcga acattttcta gagaaagcgc attcggttcg ccatcaattt
     1381 gatgcacaag ttcagcaaaa agagttagtt aaaacgcttt tacaggacgt cgcacgggag
     1441 cgagggcacg cgcttacggc aagaataaat aaaacaggtg aagttgaatt tgaacgtgcg
     1501 ccaacgcgga cgcggagtaa cacgcgaggg cacggcatgg gcattcgtga caactcacgc
     1561 gggtacgact acgaacgtta aaaggggact gtatggcgag tacagaagag gggttgctca
     1621 cgctcttgtt gtccgttgag catttgaaaa acactgcgat agaacaacag aaagcggttg
     1681 cagacacgct ggcggtcgca agggaaacac tgcgggggta tcgggatatt tctgacagtg
     1741 ttgtaaaaca ggttagcgat gaagtcagaa aaggggtaca gaaaacggac attgcggggc
     1801 tgatgggtga gcgcatgacc caggattttc agcgtcttga agagactgtg gcaaagatga
     1861 gagccaacgc ggaggcgctt aacaaggaca ttgcgacaat tcatcacaat gttgttaaag
     1921 aatacaacac gctcaataaa tacagtctga aatggtttat cggtgttacg gtcagttttc
     1981 tggtcatgtt ctcgttgctt ggctggttta ccaaaacgtt gatcgacagg aactacaccg
     2041 cgacggaggt cgtgtatcag aaaattgttg atattgaaaa gaaactggac aaaccagcaa
     2101 cagggaagag ccggaaaaac tgacgcgctt cgcttgtcca acgcctcacg ggacgcgaaa
     2161 aaattccgcg tcccgtgagg cgttttctga tgagaaagtt tgctgttttg ccccgttcgc
     2221 ccttctgaac gctcgaaaca gatcgcgcat tgctttcgtg aatagttatg caggctcatg
     2281 aaaacgattc tgacgcgttt ttctggcttt gcctggtgtt tccttgtctt tttgcgtttt
     2341 ttgcgccaga aagcgattga gggcgtttta aggggtacgt acaacaggag ttatggtaaa
     2401 tggatcgggt ttgcgggaag actcgacagg ttttgtcgtt gggggtagtg taagcgactg
     2461 aaaaacaaac gcccctgaaa ttcgtgtctc tccgccaaga ttgtcacgaa gatacagggg
     2521 cgtgtgggtc gtaacacacg gaggaatcct aacacatgag cgccgcgctt caatacttcg
     2581 aagaaaattt acctcaccgt ccctatcaca cggatgatct cgctttcggc cttcgcatct
     2641 ccggcaaagg gcgtgcgctt cttgcgcggt acatccagca gaaccagcca catgcgcagt
     2701 tctggctggt ttttgacgtt gaccgcgagg gggcagcgat tgactggagc gaccggaacg
     2761 cccccgcgcc gaacatcacc gttaaaaatc ctgtgaacgg acacgctcac ctgctctatg
     2821 cactcaatat cgccgtgaga accgcgcctg atgcgtcggt taaggcgctg aaatatgccg
     2881 cagcggttga acgttcgctg tgtgagaaac tttgtgcgga tgtgaactac agcggcttga
     2941 tttgcaaaaa tccgttccac ctggaatggc tggtgatgga gtggcgcgag gaagcctata
     3001 ccctcgatga actggctgat tatctcgatt tgagcgcctc agcgcgtcgt agcatcgata
     3061 aacattatgg gatggggcga aactgccacc tgttcgaaat gacgcgcaaa tgggcttaca
     3121 gggcgattcg tcagggctgg ccagcattct cacagtggct tgatgccgtg atccagcgtg
     3181 tcgaaatgta taacgcatcg cttcccgttc cgctttcacc ggctgaatgt cgggctattg
     3241 gcaagagcat tgcgaaatac acgcacagga acttcacgcc ggaaactttc gcacagtatg
     3301 tggctgatac gcacacgcca gaaattcagg ccgcacgagg tcgcaagggc gggaaggcca
     3361 attcgtcaaa aaatcaggcg gacaaaggga aagttggcgg gaaaaacagc ggtgttgtcc
     3421 ggtggacggc taacgatgat aaacgtcgta gggcgcttga tatgtacatc ttgggagcgt
     3481 caacagaaga tattgcagtg gcggttggtg ttagccgttg gactgttcga cgctggatgg
     3541 atagctctgt agaatggcta ctgagtaaac aaataatcaa aatttaatta acaaaaagaa
     3601 tacatacccc cccctgtttt gttagttttt tgttaatttt gggtgcacga agcaagccta
     3661 tatcagataa cagcgcctga acgggggttt ggtgtttttt tgcgcggcgt ctccgacccg
     3721 ctcccacacc cccaaaccaa ataccgaaac cctgcaaacc aagcgagtgg tggctccgct
     3781 cgccgcagcc ctagcgaccg agccgtcagg ctcgcgagtg ggcgaggaag cggaagataa
     3841 tcggaagtca cattgagcac ttacgcacct atgccagatc cgcgcgtagc gcgtcgc
//
