LOCUS       NZ_CP069663             5874 bp    DNA     circular CON 18-JUN-2023
DEFINITION  Escherichia coli O89m:H10 strain MIN12 plasmid pMUB-MIN12-5,
            complete sequence.
ACCESSION   NZ_CP069663
VERSION     NZ_CP069663.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN17831485
            Assembly: GCF_017570605.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli O89m:H10
  ORGANISM  Escherichia coli O89m:H10
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5874)
  AUTHORS   Majewski,P.
  TITLE     Plasmid mediated mcr-1.1 colistin-resistance in clinical
            extraintestinal Escherichia coli strains isolated in Poland
  JOURNAL   Front Microbiol (2021) In press
REFERENCE   2  (bases 1 to 5874)
  AUTHORS   Majewski,P.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2021) Department of Microbiological Diagnostics
            and Infectious Immunology, Medical University of Bialystok,
            Waszyngtona 15A, Bialystok 15-269, Poland
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: Unicycler hybrid assembly v. v0.4.8-beta
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 484x
            Sequencing Technology :: Illumina MiSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 06/18/2023 02:09:43
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,209
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,805
            CDSs (with protein)               :: 4,805
            Genes (RNA)                       :: 122
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 14
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 89 of 282
            Pseudo Genes (incomplete)         :: 204 of 282
            Pseudo Genes (internal stop)      :: 44 of 282
            Pseudo Genes (multiple problems)  :: 55 of 282
            Pseudo Genes (short protein)      :: 4 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP069663.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5874
                     /organism="Escherichia coli O89m:H10"
                     /mol_type="genomic DNA"
                     /strain="MIN12"
                     /serotype="O89m:H10"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:2810405"
                     /plasmid="pMUB-MIN12-5"
                     /geo_loc_name="Poland: Bialystok"
                     /lat_lon="53.12523570 N 23.15905989 E"
                     /collection_date="2018-05-05"
                     /genotype="ST-10"
     gene            join(5520..5874,1..176)
                     /locus_tag="JSU14_RS26075"
     CDS             join(5520..5874,1..176)
                     /locus_tag="JSU14_RS26075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001673158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_001673158.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTEKMLNDEFRKLEESVNRELTSNE
                     SLIRNAINDHTTALKELLERYQKTTVDTMDAHWKTVLKMSVKRWLWLIIVSVLMFATTG
                     SLLWYQGMKINANMNILREQKESLEKLNAKTWGVRYHEDSNGRFLVLPKGMKAETNWTK
                     DNGKLNAVRLVQE"
                     /name="MbeB family mobilization protein"
     gene            183..416
                     /locus_tag="JSU14_RS25600"
                     /old_locus_tag="JSU14_25640"
     CDS             183..416
                     /locus_tag="JSU14_RS25600"
                     /old_locus_tag="JSU14_25640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_021520366.1"
                     /translation="MTELERHLLNALEQLQQDYMQRLNEWENAFVELQKMFSLTQRDNA
                     MLNGRVMQLSQQVQHLSERTERLSQLYSENWR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            1136..2188
                     /locus_tag="JSU14_RS25605"
                     /old_locus_tag="JSU14_25645"
     CDS             1136..2188
                     /locus_tag="JSU14_RS25605"
                     /old_locus_tag="JSU14_25645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001557502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_021517772.1"
                     /translation="MNLQQRINKLPQLSSSFSFGKDIDNIHSFIFNETSKDKIEDLLRK
                     WVSGNQPCVFGKLASKKIKGLDFHLSIVNSPQLYNDDGHLFDFLRNERVRFKERARRGE
                     VSAHLIYFIHPQLAFARPSEELVDIQKYICSLHMPECYPIKEDVIYTESVPFQDKDGLK
                     IYKAGVNVFYSSAHRTRNHDRRIPGGILISVNAPGHFMRLAIEKGFYKDQEQALADIRN
                     MTIQSVGNGGYSHPEGISTTWHSESKLDRFGCPVHTGNSSYYSGFYHTDVLIPGELTKD
                     ERLLHEIDNSDPMIFNWNVLFYVSLEEFPIDDPYYGEFIGVPVDDASMFFNSFQPRKFE
                     NNPLYEKEDD"
                     /name="hypothetical protein"
     gene            2190..3056
                     /locus_tag="JSU14_RS25610"
                     /old_locus_tag="JSU14_25650"
     CDS             2190..3056
                     /locus_tag="JSU14_RS25610"
                     /old_locus_tag="JSU14_25650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001335763.1"
                     /GO_function="GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="WP_001335763.1"
                     /translation="MIKGMLFAFLAAFSWGAAIVMSKKGVEELDAGGVFFWQICSAVIL
                     SWIVLFIKRKKLPLNSKAILAYATGVFEPFLAYTFTLYGLYFISAGSASIIFSLESVFI
                     LVLSVLICSVKINSPQYFILFLIGAIVGSILVALPDISNGSNNIVGYCLVVAGVLSAAF
                     YVVISSRLIKSFEPITLLSGQLTFSLILSSLFIFISKSSIYLPLESVIIVFSSGILQYF
                     LAFCFYLNSLRWLPVHLAGIMLYFIPLVALFLSWLFLDEQFTFLQGVGFIVTVLSVYFL
                     NRKYSEE"
                     /name="DMT family transporter"
     gene            complement(4262..4453)
                     /locus_tag="JSU14_RS25615"
                     /old_locus_tag="JSU14_25655"
     CDS             complement(4262..4453)
                     /locus_tag="JSU14_RS25615"
                     /old_locus_tag="JSU14_25655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_021539356.1"
                     /translation="MTKQEKTALNMVRFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENR"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            4519..4842
                     /locus_tag="JSU14_RS25620"
                     /old_locus_tag="JSU14_25660"
     CDS             4519..4842
                     /locus_tag="JSU14_RS25620"
                     /old_locus_tag="JSU14_25660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_024188420.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAAWMRRVCLGEPVARSGKLPTL
                     APPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVVAALIAIGDELRRLRLAVREQGAR
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            5051..>5482
                     /locus_tag="JSU14_RS26080"
                     /pseudo=""
     CDS             5051..>5482
                     /locus_tag="JSU14_RS26080"
                     /inference="COORDINATES: protein motif:HMM:NF015397.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     Typing          4087..4168
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="98.795"
                     /coverage="100.0"
                     /evalue="6.23e-34"
     Typing          3443..3630
                     /name="rep_cluster_2401"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="4.63e-94"
     Typing          3..520
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="97.688"
                     /coverage="99.0"
                     /evalue="5.74e-81"
     Typing          4833..5872
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 atattctccg cgagcagaaa gagagtctgg agaagctgaa cgcgaagaca tggggcgtgc
       61 gttatcacga ggacagcaac ggacgttttc tggtgttgcc gaaggggatg aaagccgaaa
      121 cgaactggac gaaggacaac ggcaaactga acgcagtgag actggtgcag gagtgacaga
      181 gcatgacgga gctggaaaga catttgctga acgcattaga gcaactgcaa caggactata
      241 tgcagcggct gaacgaatgg gagaacgcct tcgtggaatt gcagaaaatg ttttcgctta
      301 cgcaacggga caacgcgatg ctgaacgggc gagtcatgca gttgagtcag caggtgcagc
      361 acttgagcga gcggacagaa cgcttgagcc agttatacag cgagaactgg agataaggga
      421 tgagcggctg atacaggagc gcgaatatgc gttatccctg gagcgggagc gccagccgga
      481 aattcaggaa cggacactgg atggcccttc gctgggatgg taaccagaga caggctaaaa
      541 tgttggcagc gcgtttccgg tgcatttttt gggataatgg ttggttcggg ggcaccggga
      601 acgcgcttca gtcagagctt gctgaaaaca caggaacgcg ttaaaacgca tgcgggcgcg
      661 attctggtgt gtctttgttg ctgtgttttg ccgttattgc cagatcgcgc tcattcccat
      721 gaatttctgt ttttccgctc cggttccgtc ttctgccttt ccgaaagtat gaaaaaaatc
      781 cgtttcagca gtctctggct ggcaattcct gcgtgattca catggctgca tagctatgca
      841 tgtggttaaa atttatcggg tgcgatcgcg gcagtttttc gggtggtttg ttgccatttt
      901 tacctgtctg ctgccgtgat cgcaccgaac gcgttttggc ggtgcgtaca atgcgacgtt
      961 atggtaaatg gagcggattt tcaggaagga gtgatactaa caactcgaac actgtgcaaa
     1021 taatgatttt ttaaaatatc ggtaacgata ataaattatt tcgttttacg tcggtaataa
     1081 atataatgta gtttcagtat ataaaaaata tatgaggtgt cttgtgggag ctttcatgaa
     1141 tcttcaacaa cgtataaata aactaccaca attatcatcc agtttttcat ttggaaaaga
     1201 tattgataat attcattcat ttatctttaa cgaaacttct aaagataaaa ttgaagattt
     1261 actaagaaaa tgggtgtcag gcaatcagcc ctgtgtattt ggtaaattgg caagcaagaa
     1321 aattaaaggg cttgatttcc atttatctat tgtaaatagc ccccaactat ataatgatga
     1381 tggtcatttg tttgattttt tgcgtaatga aagagttcgt tttaaagaac gcgcaagaag
     1441 aggagaggtc agtgcgcatc tcatttattt tattcatcca caacttgcat ttgctcgtcc
     1501 atcagaagag ttagtggaca tacaaaaata tatttgttcg ctgcatatgc cagagtgcta
     1561 tccaataaaa gaggatgtta tttatactga aagcgtacca tttcaggata aagatgggct
     1621 aaaaatatat aaggctggtg ttaatgtttt ttactcttct gctcatagaa caagaaatca
     1681 tgatagaaga ataccggggg gaatacttat ttctgtaaat gccccggggc attttatgag
     1741 gctggcgata gaaaaaggtt tctataaaga tcaagaacaa gcgttagcgg atattcgtaa
     1801 catgactatt cagtctgttg gtaacggtgg gtattcacat ccggaaggga tttctacaac
     1861 atggcattca gaatctaaat tggatcgttt tggatgccca gtgcacaccg gtaatagttc
     1921 ttattacagc ggtttttatc atactgatgt attaattcca ggagaattaa ctaaggatga
     1981 aagattgctc catgaaatag ataatagtga tccaatgatc ttcaactgga atgtattgtt
     2041 ttatgtttca ttagaagagt ttcctataga tgatccttat tatggagagt ttattggtgt
     2101 tcctgtagat gatgcatcta tgttttttaa ctcctttcaa ccaaggaaat ttgaaaacaa
     2161 ccccctttat gagaaagagg atgattaata tgattaaagg aatgcttttt gcttttttag
     2221 cagcgttctc atggggggca gctatcgtaa tgagcaaaaa aggagttgaa gaactagatg
     2281 ccggaggggt gtttttctgg cagatttgta gtgcagtaat actatcttgg atagttcttt
     2341 ttataaaaag aaaaaaactc ccattaaact caaaagcgat acttgcttat gcgacaggcg
     2401 tttttgaacc attcttagcg tatactttta cactctacgg actttatttt atttcagcag
     2461 gaagcgcatc aattattttt tcacttgaat cagtgtttat attggtgttg tctgttctta
     2521 tttgctcagt taaaattaat tccccgcaat attttatatt gttccttata ggtgcaattg
     2581 ttggcagtat tttggtagcc ttgccagaca tcagtaatgg atcgaataat attgttggat
     2641 attgtcttgt tgttgccggt gtgttatcgg cagcatttta tgttgttata tcgtctcgcc
     2701 ttattaaatc gtttgagcct atcacattat tgagcggaca gttgacattc tcccttatac
     2761 tgtcatcgct ctttattttt atatctaaat cctcaattta tcttccgttg gaatccgtga
     2821 ttattgtttt ttcatctgga atattacaat attttttagc tttttgtttt tacttgaact
     2881 ccttgcgttg gctcccagtt catttggcag ggataatgtt gtactttatt ccgctagttg
     2941 cattattttt gtcatggttg tttttggatg aacaattcac attcttacag ggggtaggat
     3001 ttattgtaac tgtgctaagt gtatattttc ttaacagaaa atactcagag gagtaacatt
     3061 ctgttcagga ttgccccccc ccggcctcca tcgataccgt gccaaatgca cgtaaaaaac
     3121 gggagcgttt ctggtgtcgg aatgtaacca aggggggcag aggcgcgcta aaacgctctc
     3181 aggggagtca aatagagacg tttgtaacaa aaagatcaaa ggatcctctt gagatccttt
     3241 ttttctgcgc gtaatctgct gcctgtaaac gaaaaaaccg ccttgcaggg cggtttgatc
     3301 gaaggttaag tcagcttgca gactgcttaa ccaggtaact gtcttatgcg agacgagtca
     3361 ccaaaattgt tcttctagtg tagccgcact ccggcagcca cttcaagtac tctctaaata
     3421 tctcgcacat gacttttacc aatggctgct gccagtggcg ctttgtggtg tcttttccgg
     3481 gttggaccca agagaacagt taccggagaa ggcgcagcag tcgggctgaa cggggggttc
     3541 ctgcatacag cccagcccgg agcgaactgc ctaagtcgcc ccgagatacc agaagacgtg
     3601 agctatgaga aagcgccacg tgtcccgtaa gggagaaagg cggaaaaggt atccggtaat
     3661 cgatatggcg ggaacgtggg ggcgcaggag ggagcgattc accggaaacg gtgatgatct
     3721 ttaagccacg tcgggtttcg ccaccgctgg cggatttcat ggttgagccg caaggctccc
     3781 acagatgcac cgaaaaagtg tctgttttgt gaagtccgtc aggggggcgg agcctatgga
     3841 aaaagcgcct ccggcgcggc cttttccggg gtttcgtttt ttgcgcacat gttctttcct
     3901 ggtttatccc cgggttctgt ggataacagg gttagcgttt ttgagaaagg agagtccgct
     3961 cgccgcagcc gaacgaccga gcgtagcgag tcagtgagcg aggaagcgga agagcgcctg
     4021 aactggcatt ttctccttac gcatctgtgc ggcatttcac acccgacatg gcgcactttt
     4081 catacaatcc gcactgatgc cgaatgatta agccagtata cactccgcta tcgctacgtg
     4141 actgggtcag ggcttcgccc cgacacccca aaggtcaacg gcctgacgcg ccctggcggg
     4201 cttgtctgct cccggcatcc gctcacagac aagctgtgac cgtctccggg agctgcatgt
     4261 gtcagcggtt ttcaccgtca tccccgaagc gtgcgaggca actgcggtaa agctcatcgg
     4321 cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagt tcgttgagct
     4381 tctccagcag cgttaatgtc tggcttctga taaagcggac catgttaagg gcggtttttt
     4441 cctgtttagt cactggatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata
     4501 ccgatgaaac gcgagaggat gctcacgata cgggtgactg atgatgagca tgcccgttta
     4561 ctggaacgct gtgagggtaa acaactggcg gcatggatgc ggcgggtctg cctgggtgag
     4621 cctgttgccc gttccgggaa gctgccgaca ctggcaccgc cgttactgcg tcagctggcc
     4681 gctatcggga ataaccttaa ccagacagcg aggaaggtga acagcgggca gtggtcttcc
     4741 ggtgaccggg ttcaggtggt ggccgcactg atagccatcg gggatgagct gcgccggctg
     4801 cgtctggcgg tcagggaaca gggggcgcgg gatgatagtt aagtttcatg ccaggggaaa
     4861 aggtggtggc agtggtccgg ttgattacct gctgggaagg gagcgtaacc gggaaggggc
     4921 aacggtgctt caggggaatc cggaagaggt ccgggaactc atcgatgcca cgccatttgc
     4981 gaagaaatac acgtcaggtg ttctgtcgtt cgcggagaag gagctgccgc ctggagggcg
     5041 tgaaaaagtg atggcgagct ttgagcgtgt actgatgccc ggtctcgaga aaaatcagta
     5101 cagcatcctg tgggtggagc accaggacaa gggacggctt gagctgaatt ttgtcattcc
     5161 gaacatggag ctacagaccg gaaaacgcct ccagccgtac tacgaccggg cagacaggcc
     5221 gagaattgat gcctggcaga cgctggtgaa tcaccattac ggtctgcatg accccaacgc
     5281 cccggagaac cgccggacgc tgacccttcc tgataacctg ccggaaacga aacaggcgct
     5341 tgctgagggc gtcacacgcg ggatagacgc gctttaccat gccggagaga taaaaggccg
     5401 tcaggatgtg attcaggcgc tcacagaggc ggggcttgaa gtggtcaggg tgacgcgaag
     5461 cagtatcagc attgcagacc cgaacggcgg gaggaatatc agactgaaag gagcgtttta
     5521 tgagcaatct tttacagacg ggcgcggagt tcgagaaaaa gctgaaagag agagccgaat
     5581 ctacagagaa aatgctgaac gacgagttca ggaagctcga agaatctgta aacagggaac
     5641 tgacctcaaa cgaatcttta atcaggaacg ctatcaacga ccatacaaca gccctgaaag
     5701 aactgctgga gaggtatcag aaaacgaccg tcgatacgat ggacgcgcac tggaaaaccg
     5761 tactgaaaat gagcgtgaaa cgctggctgt ggctgattat cgtctcagta ctgatgtttg
     5821 ccacgaccgg gagtctgctg tggtaccagg gcatgaagat aaacgcgaat atga
//
