LOCUS       NZ_CP069662            12696 bp    DNA     circular CON 18-JUN-2023
DEFINITION  Escherichia coli O89m:H10 strain MIN12 plasmid pMUB-MIN12-4,
            complete sequence.
ACCESSION   NZ_CP069662
VERSION     NZ_CP069662.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN17831485
            Assembly: GCF_017570605.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli O89m:H10
  ORGANISM  Escherichia coli O89m:H10
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12696)
  AUTHORS   Majewski,P.
  TITLE     Plasmid mediated mcr-1.1 colistin-resistance in clinical
            extraintestinal Escherichia coli strains isolated in Poland
  JOURNAL   Front Microbiol (2021) In press
REFERENCE   2  (bases 1 to 12696)
  AUTHORS   Majewski,P.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2021) Department of Microbiological Diagnostics
            and Infectious Immunology, Medical University of Bialystok,
            Waszyngtona 15A, Bialystok 15-269, Poland
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: Unicycler hybrid assembly v. v0.4.8-beta
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 484x
            Sequencing Technology :: Illumina MiSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 06/18/2023 02:09:43
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,209
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,805
            CDSs (with protein)               :: 4,805
            Genes (RNA)                       :: 122
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 14
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 89 of 282
            Pseudo Genes (incomplete)         :: 204 of 282
            Pseudo Genes (internal stop)      :: 44 of 282
            Pseudo Genes (multiple problems)  :: 55 of 282
            Pseudo Genes (short protein)      :: 4 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP069662.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..12696
                     /organism="Escherichia coli O89m:H10"
                     /mol_type="genomic DNA"
                     /strain="MIN12"
                     /serotype="O89m:H10"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:2810405"
                     /plasmid="pMUB-MIN12-4"
                     /geo_loc_name="Poland: Bialystok"
                     /lat_lon="53.12523570 N 23.15905989 E"
                     /collection_date="2018-05-05"
                     /genotype="ST-10"
     gene            join(12542..12696,1..142)
                     /locus_tag="JSU14_RS25520"
                     /old_locus_tag="JSU14_25560"
     CDS             join(12542..12696,1..142)
                     /locus_tag="JSU14_RS25520"
                     /old_locus_tag="JSU14_25560"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_039022318.1"
                     /translation="MSLYPKDKNPVFQPGGYGLRYDGQAEAIARAGEPPINYAPGQELS
                     RAAPALPEHPTHGAARAFRDHLTGEYVVAAGPVEVRTRVSAEEAALLWATLTA"
                     /name="hypothetical protein"
     gene            121..675
                     /locus_tag="JSU14_RS25525"
                     /old_locus_tag="JSU14_25565"
     CDS             121..675
                     /locus_tag="JSU14_RS25525"
                     /old_locus_tag="JSU14_25565"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_039022319.1"
                     /translation="MGYFDCVKLTDAEWPDHMVEGRDAALRQAMRGGVSQWCRDKVVQL
                     LPVLRCPRGEWRDAELKRVDELADALGLDDMGLFAYGAMIVKRRAYVAGSLSRAKPPEP
                     LAVVLDDGEHRCTVYAMPHPTLGVPEISERGEDGYWTISAGGCAWIKARVSLGEGVRLW
                     NELVNCARYPDAPVLTRGMLE"
                     /name="hypothetical protein"
     gene            complement(899..3658)
                     /locus_tag="JSU14_RS25530"
                     /old_locus_tag="JSU14_25570"
     CDS             complement(899..3658)
                     /locus_tag="JSU14_RS25530"
                     /old_locus_tag="JSU14_25570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008324017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="WP_053266538.1"
                     /translation="MAIKIPVSAQFDAADLQQQIKMVNDQIRILASQVGNANKQKWEPI
                     NLKSKDDLQGFIKQMDLLMKKQTDFATKMKQTGQGGNPLFADFKKMAEGRLGEQIKYME
                     SVLGWAGVEFSNLPAPKPPKKPPVVPPAPPSPPEPAWRKELGRQGMSGLHSILGNAGPI
                     GGTISNALNTGMSSGASAGMAGLVGGLAALGVGKIIGAIADKIGQAQDNAIGLDKLYRQ
                     VGGMVAYSNLRSGVGDIANRLGMNNADAIGMASTYTRASNLRPGMNLGTGLLVSGGLAR
                     GYGLDPNSVAASFGSLGATGVTNSDQSIRRTGMMMGEAIAKSGAFAQAGDAMTAITDFA
                     NTQARMSLSQPNIAGYGGALAGLMSSGVPGMDLQGAGSLLNRMNGAVMHGGNMGEASQA
                     FSARLGLRRGINDPMALQALQEGGAFSTVSSMLGKGSLYNQTTGKSFSGNETMLDMIRH
                     DIHRQYGYGSNATKALANHLGIGVNEAMLVDQMSSRDINGASSRLKRLGIDEKSINMSS
                     LPVLGQIESGKGLEALGNQYLKRSDLSKEERRGLIDALKGANPEKLKDALAEVASKHGG
                     TTTEGSQIRDNIAKLNNTLEDYSKRALPALDMMRMALVNMTGGSEASVRKQYYDSNMRE
                     ASSEIGAKYKDRRAAIQSQIDALTAKGVGLQSSGPDREKLVKLKAEMQSLNDQEGADRT
                     RAAERIKLQTYGASSSTEAALSTGDGYIQSPAAAAANGGGGKYRLNSDMKKRLERLRPY
                     FEEAGRKYNIDPRRLEAIAATESGFNNNLTSSKRAQGIMQVLPTSAPGANLSDERENIM
                     AGAMVYSQMRDRYGYLGQDEILRGYNGGSRRGSAENVAYPGKVNSYYRDLAQVDDMTPG
                     SNGNKGAVGEMYGTMRVVHEDKEGKQVKPPESVSMSGTFIQPSSWGNR"
                     /name="transglycosylase SLT domain-containing protein"
     gene            4045..4224
                     /locus_tag="JSU14_RS25535"
                     /old_locus_tag="JSU14_25575"
     CDS             4045..4224
                     /locus_tag="JSU14_RS25535"
                     /old_locus_tag="JSU14_25575"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_039022310.1"
                     /translation="MKFRASAICDLMGVSRRQLDRWIKAGTFPLPDGRDAKGYWWEADR
                     VATRMISERKERGM"
                     /name="hypothetical protein"
     gene            4227..5366
                     /locus_tag="JSU14_RS25540"
                     /old_locus_tag="JSU14_25580"
     CDS             4227..5366
                     /locus_tag="JSU14_RS25540"
                     /old_locus_tag="JSU14_25580"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_052249901.1"
                     /translation="MVNERRDYLFDHAVFSAALARVDPDPSVMGWLAQARTLACWCELL
                     GMDPLETCVEWSARGVNAGKGDRAMLVSALRDLDELDRLAAAGAFLRSSGVAADVAAGR
                     IAVREPSEDELAARQAHLVALRERAAAAAEKLTEARIKATGRARMLLHRATKPGSAALY
                     WQAKERAACLPLPCPDDVKCSDWRTFLPVIGQVYWNSSLLVPLYGVNESLRLEQMSVEV
                     ICGRANPEAAGSLGEKRGLKNAPREGLFYPFDLDAVGSGAPVVVCEGFMSGLALSLLIG
                     GKLPVVCAMSVGNFGATVQSLNKFVMGGHPFVLVPDIGGQDLAIDQAIPNARVIDLSAV
                     PGAEGVDGYDPFDLLARHGVEMGRKYLRGLFREARDASL"
                     /name="hypothetical protein"
     gene            5433..5714
                     /locus_tag="JSU14_RS25545"
                     /old_locus_tag="JSU14_25585"
     CDS             5433..5714
                     /locus_tag="JSU14_RS25545"
                     /old_locus_tag="JSU14_25585"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_052249902.1"
                     /translation="MTDQTTTVADLALSQSAFLVSQAIVELDAMFGEGYAAAHPAVLAQ
                     FLQTAGVIYATQLQATAAFDLAQQLDRLIGLGLGVEAGLLQAAQGASH"
                     /name="hypothetical protein"
     gene            complement(5774..6301)
                     /locus_tag="JSU14_RS25550"
                     /old_locus_tag="JSU14_25590"
     CDS             complement(5774..6301)
                     /locus_tag="JSU14_RS25550"
                     /old_locus_tag="JSU14_25590"
                     /inference="COORDINATES: protein motif:HMM:NF013561.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix domain-containing protein"
                     /protein_id="WP_208284786.1"
                     /translation="MKRKASTFGLRLPPELLEQVNELAEKTNRTRTDVITDALRAYLGI
                     QEPQGESGNRLDLMVELLQDIASTLKHSVPQKATRHAVAPRAEKQYRSTTEASREAQRP
                     AGAEISGHYDEAEVMATIRRMREEQRDRGFRYDNKAIAQALNEAGLLQSNGRPWNNDRI
                     NTVITRRMPDLK"
                     /name="ribbon-helix-helix domain-containing protein"
     gene            6821..8290
                     /locus_tag="JSU14_RS25555"
                     /old_locus_tag="JSU14_25595"
     CDS             6821..8290
                     /locus_tag="JSU14_RS25555"
                     /old_locus_tag="JSU14_25595"
                     /inference="COORDINATES: protein motif:HMM:NF040238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5906 domain-containing protein"
                     /protein_id="WP_104691665.1"
                     /translation="MSKTEQKTATVTPLDGFSWVAPENRTFSVNPLLDRAKASFRANAL
                     SAYYGAAYVSSDDARNIHVFDGSVWELFPTARIEYDAAALLEYAGADFDPQEILGIVRV
                     MRMKNPRMGNESPDLISFDNGVLDISTGVFRPHSPFDWLTTKNGVVWVPGSDDETLETH
                     APSFNKWLVHASRGDKTRATAIMAAFYMVLTCRHDWEMYLSVSGPGGSGKSTMQRVCTM
                     LAGEHNTSAFSLTGICGDFGLEHLMGKRFLVNSESESKWPARAVSLVKAITGGDLLPVN
                     RKGEKVVSATLKAIMMFVGNVARSADENDNGGLARREIPFHFPDVVADADKDTALTEKI
                     RAELPVIIRRLLARFPEANMAKAALLKQRDGADAKAIKLECSPLERFLSSLEIDPLATL
                     TGSGGMQWNDTGLNSAGTVRYERRFVMHAYLSFCGYEGIKPSMTKAELVRAIKFRFGSA
                     LKTGKGAKNRTLVNLRVREDAEYLAGVTDAE"
                     /name="DUF5906 domain-containing protein"
     gene            complement(8977..9219)
                     /locus_tag="JSU14_RS25560"
                     /old_locus_tag="JSU14_25600"
     CDS             complement(8977..9219)
                     /locus_tag="JSU14_RS25560"
                     /old_locus_tag="JSU14_25600"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_060634175.1"
                     /translation="MAFENIERRFQRWKVIMVPGAKSYESWDEMVTGENFPEEARYKYK
                     THRTKAIDLTLKNPDYVHTMSENGITVRYRLERWR"
                     /name="hypothetical protein"
     gene            complement(9239..9589)
                     /locus_tag="JSU14_RS25565"
                     /old_locus_tag="JSU14_25605"
     CDS             complement(9239..9589)
                     /locus_tag="JSU14_RS25565"
                     /old_locus_tag="JSU14_25605"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_060634174.1"
                     /translation="MRTKAIIAAALLATAGTAAAAPVATTYICNDGTFIHLNEQNGRLT
                     ASWHGADIPVTKIETSKLTASLNGEQGSSTLQLVVGYNTTPNPIAMITTIVNGSMNSQS
                     CKPDTASKHIDL"
                     /name="hypothetical protein"
     gene            complement(9690..9881)
                     /locus_tag="JSU14_RS25570"
                     /old_locus_tag="JSU14_25610"
     CDS             complement(9690..9881)
                     /locus_tag="JSU14_RS25570"
                     /old_locus_tag="JSU14_25610"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_060634173.1"
                     /translation="MTPGRTQILKATADIAKALGTDTADVERHFLQHAVVLARLRQEGK
                     RPRVKAWMAENIGTPSAM"
                     /name="hypothetical protein"
     gene            complement(9884..10075)
                     /locus_tag="JSU14_RS25575"
                     /old_locus_tag="JSU14_25615"
     CDS             complement(9884..10075)
                     /locus_tag="JSU14_RS25575"
                     /old_locus_tag="JSU14_25615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008324022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_096321248.1"
                     /translation="MQIIQQTNPARYPGLSIEHGTITQHGRARAVIMNRRGIHQVSLRT
                     AEGRTVTLPVCRLFQWEA"
                     /name="hypothetical protein"
     gene            complement(10185..10826)
                     /locus_tag="JSU14_RS25580"
                     /old_locus_tag="JSU14_25620"
     CDS             complement(10185..10826)
                     /locus_tag="JSU14_RS25580"
                     /old_locus_tag="JSU14_25620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016241263.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WP_208284785.1"
                     /translation="MITNTTAVAYMRASTQEQDANRAREEITLFAKEHGITIRGFYAEN
                     VSGTYLERPELNRLLEEAKPHDILLLEKLDRLSRLSAEEWSLLKERIRTKRLRIVCIDI
                     PTTLAVLQGGQPVGAGGFDVRRVVLDALNDMLLDIAAAWARDDYETRRKRQQQGIEKAK
                     KLGRFKGKQKDEELRGKIRTLLDTSSMSQNQIAKLIGCSRNTVSRVVKGE"
                     /name="recombinase family protein"
     gene            11091..11777
                     /locus_tag="JSU14_RS25585"
                     /old_locus_tag="JSU14_25625"
     CDS             11091..11777
                     /locus_tag="JSU14_RS25585"
                     /old_locus_tag="JSU14_25625"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_039022316.1"
                     /translation="MENEQIAPVDFEGANRIYLADVLAEACSALPELPDLPVPSLDGDD
                     AAVASLLHLMRQRDEMASAIGVLNFELGKLKEAAEAAHDRLVDAEARIAAARDALLAGN
                     EPEPRDAMLALVVDQIRADVQAADAAVDIKSREVGALDAQMNTAVHQIEGAIDELICSC
                     RIARARALELALLNELRALAVHDGDKNRFNRAFVPAPELALLSSNYGQIEMLRPGRMPH
                     LNRGVL"
                     /name="hypothetical protein"
     gene            11777..12175
                     /locus_tag="JSU14_RS25590"
                     /old_locus_tag="JSU14_25630"
     CDS             11777..12175
                     /locus_tag="JSU14_RS25590"
                     /old_locus_tag="JSU14_25630"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_052249905.1"
                     /translation="MSMFDQLVARLRDEQALTKSLNPGDALAAVGNGDANAPVLDQRAL
                     TIGVAPGVVACHNARLNQIADAQLLQEVRASGVVSAADAVALDNALAASRHLPPEARAA
                     GMESVRQQLNAAAAAPLSPAARGINWDE"
                     /name="hypothetical protein"
     gene            12163..12531
                     /locus_tag="JSU14_RS25595"
                     /old_locus_tag="JSU14_25635"
     CDS             12163..12531
                     /locus_tag="JSU14_RS25595"
                     /old_locus_tag="JSU14_25635"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_039022317.1"
                     /translation="MGRVKGLPRHLQGKTRLPLLGGCFSKGHRLALLAVMPIIEARPGE
                     RFDGEAAKLALFQDLLLKAGQPPAFALHEPSLYRFGAALRNNRRGLTDTGIEKANELLD
                     QHLFSRALDAIVATKQTD"
                     /name="hypothetical protein"
ORIGIN
        1 tgagcacccg acgcatggcg ctgcgcgtgc gttccgtgat cacctgacgg gcgagtacgt
       61 cgtcgccgcg ggcccggtcg aggttcgtac ccgcgtatcg gctgaggagg ccgcgctgct
      121 gtgggctact ttgactgcgt aaagctgacc gacgcggagt ggcctgatca catggttgag
      181 gggcgtgatg ccgcccttcg ccaggccatg cgtggtggcg tctcccaatg gtgccgggac
      241 aaggttgtcc agcttctccc ggtgctgcgg tgcccccggg gcgagtggcg tgatgccgag
      301 ctgaaacgtg tagacgagct tgctgatgcc ctggggctgg acgatatggg gctgtttgcc
      361 tacggcgcta tgatcgtgaa gcggcgggct tacgttgctg gtagtctctc tcgggctaaa
      421 ccgcccgagc cgctggctgt tgtcctcgat gatggcgagc atcgttgtac tgtctatgca
      481 atgccccacc cgacgctcgg cgtgccggag atctccgaac gcggcgagga tgggtattgg
      541 actatctccg ctggcggttg cgcatggatc aaggctcgcg tctcgctcgg ggaaggagtc
      601 aggctttgga acgagctggt taattgcgcc cggtaccctg atgcgccggt cctgacgcgg
      661 gggatgctgg aatgagcgag tttgctgtat cccgtctggt gatcccccat gtggtcatcc
      721 ctcccgccgt tgtcgaggct cgacgcgttc gcggtgttgc tgcagggcac gccagaaagc
      781 gccgacttgc cgaggtcatg gcatcaccta cctggtactg gtcgcggcgt gaatctcccc
      841 gtctggccac cacaaaaaaa ggggcgtaag cccctctctt ttgggcgctg ctgcgccgct
      901 accggttgcc ccagctgctt ggctggatga acgtgccgga catgctgacg ctctctggcg
      961 gcttgacctg cttaccctcc ttgtcctcat gaacgacgcg cattgtgccg tacatctcgc
     1021 cgacagcgcc tttgttgccg ttgctgcccg gggtcatatc gtcaacctgt gccagatcgc
     1081 ggtagtagct gttcaccttg ccggggtagg cgacgttctc tgcgctgcca cggcggctac
     1141 ctccgttgta tccgcgcagg atctcgtcct gtcccaggta tccgtatcgg tcacgcatct
     1201 ggctgtagac catggcaccg gccatgatgt tctcgcgttc gtcgctgagg ttggctcccg
     1261 gggcgctggt tggcagtacc tgcatgatgc cctgcgcacg tttgctgctg gtcaggttgt
     1321 tgttaaaccc ggactcagtg gcggcgatgg cctcaaggcg acgtggatcg atattgtact
     1381 tgcgtccggc ctcctcgaag taagggcgca gacgctccag gcgtttcttc atgtcgctgt
     1441 tgaggcggta tttgccgccg ccaccgttgg ctgctgctgc cgctggtgac tggatgtatc
     1501 cgtcaccggt agacagcgcc gcctctgtcg aggacgacgc cccgtatgtc tgtagtttga
     1561 tgcgttctgc ggctctggtg cggtcagccc cttcctggtc gttcaggctc tgcatctcgg
     1621 ccttgagctt gaccagcttc tccctgtccg gaccggagga ctggaggccg acgccttttg
     1681 ctgtcagggc atcgatctgg ctttggatgg cggcacggcg gtctttgtac tttgcgccga
     1741 tctcactgct ggcttctctc atgttgctgt cgtaatactg cttacgaacg ctggcttctg
     1801 agccgcctgt catatttaca agagccatgc gcatcatgtc gagggctggc agggcgcgtt
     1861 tcgagtagtc ctcgagggtg ttgttgagtt tggcgatgtt gtcgcggatc tggctcccct
     1921 cggtggtggt accgccgtgc ttcgaggcga cctctgcgag cgcgtctttg agcttctccg
     1981 ggtttgcgcc tttaagggcg tcgatcagcc cgcgccgctc ctctttggac aggtcgctgc
     2041 gcttgaggta ctggttgccg agtgcctcca ggcctttgcc gctctcgatc tgcccgagga
     2101 cggggaggga gctcatgttg atgcttttct cgtcgatacc gaggcgctta aggcgggacg
     2161 acgcgccgtt gatgtccctg ctgctcatct ggtcgacgag catggcctcg ttgacgccga
     2221 tgccgaggtg gttcgccagt gccttggtgg cgttggagcc gtatccgtac tggcggtgga
     2281 tgtcatgacg gatcatgtcg agcatggttt cgttgccgct gaagctcttg ccggtggtct
     2341 ggttgtagag gctccccttg cccagcatgc tgctgacggt ggagaacgct cccccctcct
     2401 gcagtgcctg gagcgccatc gggtcattga tgccccggcg cagtccgaga cgggcgctga
     2461 atgcctgtga cgcttccccc atgttgccgc cgtgcatgac ggctccgttc atgcggttta
     2521 agaggctacc ggctccctgc agatccatgc ccgggacgcc ggaactcata agtccggcca
     2581 gtgcgccgcc gtacccggcg atatttggct gtgacaggct catgcgtgcc tgggtgttgg
     2641 caaagtcggt gatcgccgtc attgcatcgc cagcctgtgc aaacgcgcca gacttggcga
     2701 ttgcttcccc catcatcatg ccggttcgac ggatgctctg gtcgctgtta gtgacgcctg
     2761 tcgctccgag ggaaccaaag gaggcggcga cactgttcgg atcgagtccg taaccgcgtg
     2821 ccagcccgcc gcttaccagt agccctgtcc ctaggttcat gccggggcgc agattggatg
     2881 cgcgggtgta ggtagacgcc atgccgatcg cgtctgcgtt gttcatgccg agacggttgg
     2941 cgatgtcgcc gacgcctgac cgcaggttgc tgtacgccac catgccgccg acctggcggt
     3001 acagtttatc cagcccaatg gcgttgtcct gcgcctgtcc gatcttatca gcgatagcgc
     3061 cgataatttt gccgacgccc agcgccgcca gaccgccgac aagacccgcc atgcccgcac
     3121 tcgcgccgga agacataccg gtgttcaggg cgttgctgat agtaccgcca atagggccgg
     3181 cattgccgag gatgctgtgg aggccactca ttccctgtct ccccagctct ttgcgccacg
     3241 cgggttctgg cggcgacgga ggcgctgggg gcacgacagg cggctttttg gggggctttg
     3301 gtgctggcag gttgctgaac tctacgcccg cccaccccag cacgctctcc atgtacttga
     3361 tttgctctcc gaggcgacct tcggccattt tcttgaagtc ggcaaacagg gggttaccgc
     3421 cctgcccggt ctgtttcatt ttggtggcaa agtcagtctg tttcttcatg agcaagtcca
     3481 tctgcttgat gaagccctgc aggtcgtcct tgctcttgag gttgatgggt tcccacttct
     3541 gcttgttggc gttacctacc tggctggcca gaatgcggat ctggtcattg accattttga
     3601 tctgctgttg gaggtcggca gcgtcaaact gcgccgagac cggaattttg atagccatag
     3661 ttatctctct ctgttgtaag cctcctcatg ctggcgtcac gacgtttggg gttaaatgtc
     3721 taaaacgtgt ctaaaacgca atgcgtcgaa atcgacgcgc aagccgctcc acgtctggat
     3781 tctttgaaaa atagaacttg taaaatgtct aaaacgagtc taaaacagac accatccaat
     3841 agcagttgga taaacatggc ccggatcgct cgggtgcctg gacttcggtc tacttccggt
     3901 ggcgcacttc cacctggtga aaagcgagta agcgcgaatg gcgcagccct gtcgctccgg
     3961 cgataagccc ataaggactg catcgcaata gtcatcactc aaagccccga ccactcacgg
     4021 cggggctttt ctacaggggg ttccatgaag tttcgtgcaa gcgcgatctg cgacctgatg
     4081 ggtgtgtccc gtcgtcagct cgatcgctgg atcaaggcgg gcacctttcc tctgccggac
     4141 gggcgcgacg caaaggggta ctggtgggag gctgaccggg tggccacgcg gatgatctcc
     4201 gaacgcaaag aacgggggat gtgattatgg tgaacgagag acgagattac ctttttgacc
     4261 acgcggtgtt ctcggcggcg ctggcgcgtg tagacccgga tccgtcggtt atgggctggc
     4321 tggctcaggc tcgcacgctg gcgtgctggt gtgaactgct ggggatggat ccgctggaga
     4381 cctgtgtgga gtggtctgcg cgtggtgtga atgcgggaaa gggcgacagg gctatgttgg
     4441 tgtcggcgct gcgtgacctg gatgaactcg accggctggc ggctgctggg gcgttcctgc
     4501 gctcctccgg ggtcgctgct gacgttgccg cggggcgtat tgctgtgcgt gagccgtccg
     4561 aggacgagct ggcggctcgc caggcgcatc tggttgcgtt acgggaacgt gcggcggctg
     4621 ctgcggagaa gctgacggag gctcgcatta aggcaaccgg tagggcgcgg atgctgctgc
     4681 atcgcgcaac caaaccaggg agtgctgcgc tgtactggca agccaaagag cgggcggcgt
     4741 gtctcccttt gccgtgcccg gatgacgtga agtgctcgga ctggcgcacg ttcctgcctg
     4801 taatcggtca ggtgtactgg aacagctcgc tcctggtgcc gctgtacggg gtgaatgagt
     4861 ccctgcggct ggagcagatg tcggtcgagg tgatttgcgg tcgggctaac ccggaggcgg
     4921 cgggttcgct gggcgagaag cgcgggctca agaatgcgcc gcgtgagggg ctgttctacc
     4981 cgttcgatct ggatgctgtc ggttccgggg ctcctgtggt ggtctgcgag gggttcatgt
     5041 cggggctggc gctctccctg ctgattggcg gcaagctccc ggtggtctgc gcgatgtcgg
     5101 tgggtaactt cggggcaacg gtacagtcgc tgaataagtt tgttatgggg ggccacccgt
     5161 tcgtactggt accggacatt ggcgggcagg atctggctat cgatcaggct atcccgaatg
     5221 cccgggtgat cgacctgtcg gcggtaccgg gagccgaggg cgtggacggc tacgacccgt
     5281 tcgatctgct ggcgcgtcac ggcgtcgaga tgggtcgcaa gtacctgcgg ggcttgtttc
     5341 gggaggcgcg tgacgcgagc ctgtagtggt accagttttc tctctcgttg tgcccagccc
     5401 gcttcagcgg gcttttattt gaggatctcc ccatgaccga ccagaccacc accgtcgccg
     5461 acctggcgct ctcacaatcc gctttcctcg tctctcaggc tatcgtcgag ctggacgcca
     5521 tgttcggcga gggctatgcc gctgctcacc cggctgtgct ggctcaattc ctgcagacgg
     5581 cgggggtgat ctacgccacg cagctgcagg ctactgccgc gttcgacctc gcccaacagc
     5641 tcgatcgcct gatcggcctt gggctgggtg ttgaggctgg cttgctgcaa gcggcgcagg
     5701 gtgcctcgca ctaaaacgcc ggggccggct gcgatcagtc ctttgccctg ggcggcggct
     5761 gccgcgctac cggttacttc aggtcaggca tccggcgggt gatcaccgtg ttgatgcggt
     5821 cgttgttcca tgggcgaccg ttcgactgca ggagccctgc ctcgttcagc gcctgggcga
     5881 tagctttgtt gtcgtagcgg aatccccgat cgcgctgctc ctccctcatg cgccgtatgg
     5941 tggccatcac ctcggcctcg tcgtaatgcc ccgaaatttc cgcgccagcc gggcgctgtg
     6001 cctctctcga tgcctcggtc gtgcttcggt actgtttttc cgctcgtggc gctacggcgt
     6061 gtctggtggc cttttgaggc acgctgtgct tcagtgtgct tgcgatatcc tgcagcagct
     6121 caaccatcag gtcgaggcgg ttgccgctct ccccctgtgg ctcctgtatg ccaaggtaag
     6181 cacggagtgc gtcggtaatg acatcggtgc gggttcggtt ggttttctct gccagctcgt
     6241 tcacctgctc gagcagctcc ggggggaggc gtaggccaaa ggtagatgct ttgcgcttca
     6301 tgcgtgtcgc tcctgtgctg tactggtgcc acgaattgaa gcatgtttgt gctcccacgt
     6361 ctatttcgac gtgcttctcg agtgctccac aatcggtacg ggtcgagccc ttcggctcgc
     6421 ctctctggtg cctgtggaca agtgttgtgg acaatattag gtgaagtgga taacgcgagc
     6481 gatgacgcgg gctctggcgc tcgacagcgc gagaagtggt cggcggtcag tggcgtgaag
     6541 gtctgggatc ggttaagctg atcgtcgttc gtggatcttg ttgggtgatc tacgggcgca
     6601 aaaaaagcac ctggccgggt gcttaattta ccggggttaa accccgtagt ttcgagtcag
     6661 tgtcttaacg ggacgaactc gaaggaaatc atacatcacg ttgggtgatt ctcaaatcct
     6721 cgctgagaat tgtatcacgt cgataagatc gtgcaagtcg atttcctccg ctaaagtccc
     6781 cgcctgacgc cgactcctgc gtttttaaag gagtaattgc gtgtcaaaaa ctgaacaaaa
     6841 aactgccacc gtgacgccgc ttgacggctt ttcgtgggta gcacctgaaa atcggacttt
     6901 ttcggttaac ccgttgcttg atagggctaa ggcttcattt cgcgctaacg ccctgagcgc
     6961 ctactacggc gcggcgtatg tatccagtga tgacgccaga aacatccatg tctttgacgg
     7021 ctctgtgtgg gagctgttcc ctactgcccg catcgagtat gacgcggcgg cgctgctgga
     7081 gtacgccggg gcagacttcg acccgcagga aatcctgggc attgtccggg tgatgagaat
     7141 gaaaaacccg cgcatgggga acgagtcacc agacctgatc agtttcgaca acggcgtgct
     7201 cgacatctcg acgggggtat ttcggcctca ctcaccgttc gactggctga caacgaagaa
     7261 tggcgtggtg tgggtgcctg gctccgacga tgagacgctg gagactcatg cgcctagctt
     7321 caacaagtgg ctggttcatg ccagtcgtgg cgacaaaacg cgtgcaacgg cgattatggc
     7381 ggcgttctac atggtgctga cctgccgcca tgactgggag atgtatctgt ccgtctctgg
     7441 cccaggcggg tctggtaagt ccaccatgca gcgcgtctgc accatgctgg ctggtgagca
     7501 caatacctcc gcgttctccc tgacggggat ctgcggcgac tttggtcttg agcacctcat
     7561 gggtaagcgg ttcctcgtta actccgaatc agagagcaag tggccagctc gcgctgttag
     7621 cctggtgaaa gcaattaccg gcggtgactt gctgccggtg aaccggaagg gtgagaaggt
     7681 ggtaagcgcc acgctgaaag cgatcatgat gttcgtgggt aacgtagccc gttccgctga
     7741 tgaaaacgac aacggtggac tcgcccgccg cgagatccct ttccacttcc ctgacgtggt
     7801 cgccgatgcc gacaaagaca cggcgctgac cgaaaaaatc cgcgctgaac tccctgtcat
     7861 cattcgccga ctgctggctc gcttccctga ggccaacatg gcgaaggctg cactcctgaa
     7921 gcagcgagac ggggctgacg ccaaggcgat caagctcgag tgctcaccgc ttgaacggtt
     7981 cctctcctcg ctggaaattg acccactggc cactctgaca ggttctggtg gtatgcaatg
     8041 gaacgatacg gggctgaact ctgcaggtac cgtgcgttat gagcggcggt tcgtgatgca
     8101 cgcctacctg tcattctgcg ggtatgaggg tatcaagccg tccatgacca aggccgagct
     8161 ggttagggcg atcaagttcc gcttcggttc cgcgctgaaa actggtaaag gggcgaaaaa
     8221 ccgcacgctg gttaaccttc gggtgcgtga ggatgccgag tacctagctg gggttaccga
     8281 tgctgaataa agtaacccgg ctctgaaaaa agtaaccctt ttcagaaaag taacccgtaa
     8341 gtaacccgat aaaaaacagc taagccactg ataaagtggc tttttttttg attttgttag
     8401 ctttgggtta caaggttaca tgacaaaaat aacccctcac atatgcgcgt gcgcgtatat
     8461 ctgacttaaa ttaagtaact ttgtaaccta aaagtaaccc tttccttgat ttatgcgggc
     8521 tgtaggtctt ttttgtgggt tactttgtgg gttacttttt tttgtgcggg ttactttttc
     8581 gatccccgga gatcctttcg cgccacgcag ctgcaccctg cgggatgcac tttgtggtcg
     8641 ctctccccgg tgcgtggttt gcacctgccg gatctccagc taaaaaagcc cggagggaaa
     8701 tgggggcagt ttccagtgct ggcgggggtt ggcgggtggt ttatgcacgg tggctcgtgg
     8761 ttggcaccgt gcattggtgt ttggcggcaa tcgccgcgcc taccggttag ccaaatcctg
     8821 ccccaacttt cagggggtac aggggcttaa atggggtgct gtcgtgtcgt tgggttggtt
     8881 cggggttgag gcgcttagaa tggctctggt gcgttctggt gggggcgtgc tttctaaccg
     8941 gtaggggcgc agctgtcgcc gcgctcctgg ttggggttag cgccagcgtt cgagccggta
     9001 cctgacagtg atcccgttct ccgacatggt gtggacgtag tccgggtttt tcagcgtgag
     9061 gtcgatcgcc ttggtgcggt gcgtcttgta tttgtaccgg gcttcctcgg ggaagttctc
     9121 gcctgtgacc atctcatccc atgactcgta tgacttcgct ccgggaacca tgatcacctt
     9181 ccatctctgg aaccggcgct cgatgttttc aaatgccatt gctctctcct tcgtgttgct
     9241 ataggtcgat gtgcttactt gcggtatctg gcttgcagct ctggctgttc atgctgccgt
     9301 tgacgatggt ggtaatcatg gctatcgggt tgggtgtggt gttgtacccg acgacgagct
     9361 gcagtgttga gctgccctgc tcaccgttga ggctggcggt tagcttgctt gtctcgatct
     9421 tggtgacggg gatgtcggca ccgtgccagc tggcggtgag tctgccattc tgctcgttaa
     9481 ggtggataaa tgtcccgtcg ttgcagatgt aggtcgtggc gactggtgcg gctgcggctg
     9541 ttcctgcggt ggcgagaagg gctgcggcga tgatggcctt ggttctcatg ctgtgtcctg
     9601 tgggggcgct cctcgcgccc cgggtgtatg gttttcgtta atgcgtaagt aaaaccgtat
     9661 gctgttttac cttaacgttt aagtttttct tacattgctg acggggtgcc gatgttctct
     9721 gccatccacg ctttgacgcg ggggcgcttg ccctcctgcc ggagccgtgc gaggacgacg
     9781 gcgtgctgca ggaagtggcg ctctacgtcg gcggtgtcgg taccgagtgc tttggctatg
     9841 tcggcggtgg ctttcaggat ctgcgttctg cctggggtca tgatcacgcc tcccactgga
     9901 agagtcggca aacgggaagg gtgacggtgc gcccctctgc cgttctgagg ctgacctggt
     9961 ggatgcctcg gcggttcatg atgacggcgc gtgcgcgtcc gtgctgggtg atggtgccgt
    10021 gctcgatgga taggcccggg tagcgggcag ggttggtctg ttgtatgatc tgcatgtcgt
    10081 ttacctttgc tggttgatga tgcccgggtc gcttacgagg gcaagcccgg gctggggttg
    10141 cggcggttag gcggttggag ccacctttgc cgccgcgctc tttcttactc gcctttgacg
    10201 acccggctca cggtgttccg tgaacagccg atcagcttgg cgatctggtt ctggctcatg
    10261 ctggatgtgt ccagtagggt gcggatcttg ccccgcagct cctcgtcttt ctgtttccct
    10321 ttgaaccgtc cgagtttctt cgctttctcg atgccttgtt gctggcgctt ccgtcgcgtc
    10381 tcgtagtcgt cccgcgccca tgctgcggcg atgtcgagca gcatgtcgtt cagggcgtcg
    10441 agtaccacgc ggcgaacgtc aaacccgccc gcgcctactg gctggcctcc ctgcaggacg
    10501 gcgagggtgg tcggtatgtc tatgcaaacg atgcggaggc gcttggtgcg gatgcgctct
    10561 ttcaggaggc tccactcctc ggcgctgagg cggctcaggc gatcgagctt ctcaaggagg
    10621 aggatgtcgt ggggcttggc ctcctccagt agccggttga gttcggggcg ctccaggtag
    10681 gtgccggaca cgttctcggc gtaaaagccc cggatggtga tgccgtgctc tttggcgaag
    10741 agggtgatct cctcgcgggc gcggttcgcg tcctgttcct gggtgctcgc tcgcatgtag
    10801 gcgacggcgg tggtgttggt gatcatcgcg ggtgtcctct taaacttccc tgtcaccatc
    10861 atgctcaata agagctatta actcaactta taagatgcag atatcgcgtc ttttttgcgg
    10921 cgttttcctg ctcaggtggc gtgtactcta aaggtgccgc tttcgacgcc ccgcctttgg
    10981 ggtgcggcaa ggccgcgctg cctaccggtt ctactctccc gcgctggctc cagacctgtc
    11041 gtgacgcaac gataggcgta gccgttccgg ctaaattgag agagagaact atggaaaacg
    11101 aacaaatcgc ccccgtcgat ttcgagggcg caaaccggat ctaccttgca gacgtgctcg
    11161 ctgaggcatg ctctgccctc cctgaactgc ctgatctccc tgtgccgtcg ctggatggcg
    11221 atgatgctgc tgttgcgtcc ctgcttcacc tgatgaggca gcgtgacgag atggccagcg
    11281 cgatcggtgt gctcaacttc gagctgggta agctgaagga agccgccgaa gctgcgcatg
    11341 accgcttggt tgatgctgag gcgcgtatcg ccgccgcccg tgatgctttg ctggctggca
    11401 acgagcccga gccccgtgat gcgatgctgg cgctggttgt cgaccagatc cgcgctgacg
    11461 tgcaagccgc cgatgctgcg gtggatatta agagccggga ggtgggggcg ctcgatgcgc
    11521 aaatgaacac cgccgtgcat cagatcgagg gtgccattga cgagctgatc tgctcctgcc
    11581 gtatcgcccg ggctcgtgct cttgagctgg ctctgcttaa cgagctgcgt gctctggctg
    11641 tgcatgacgg cgacaaaaac cgctttaacc gtgcattcgt tccggctcct gagctggcgc
    11701 ttctgtcgtc gaactacggc cagatcgaga tgctccgtcc ggggcgtatg cctcacctga
    11761 atagaggggt gctgtgatgt ccatgttcga tcaactggtt gctcgtctgc gcgacgaaca
    11821 ggctctgacc aagtccctta acccgggcga cgcgctggct gcggtcggca atggcgacgc
    11881 gaatgcaccc gtgctggatc agcgtgcgct gaccattggt gtcgctcctg gtgtggtggc
    11941 gtgccataac gcccgcctga accagatcgc cgacgcgcag ctgctgcagg aggttcgcgc
    12001 ctctggtgtg gtgtctgccg ccgatgcggt ggcgctcgac aatgcgctgg ctgcttcgcg
    12061 tcacctgcct cctgaggctc gtgctgcggg catggaatcg gtaaggcagc agctgaacgc
    12121 tgccgctgct gctcctctca gtcctgctgc ccggggtatc aattgggacg agtaaaaggc
    12181 ttgccgcgtc acctgcaggg caagacgcgg ctcccgttgc tgggtggttg cttctcgaag
    12241 gggcaccggc tggcgctgct ggctgtcatg ccgatcatcg aggcgcgacc tggtgagcgg
    12301 ttcgacgggg aggcggcgaa gctggcgctg ttccaggatc tgctgcttaa ggcggggcaa
    12361 ccgcccgcgt tcgcgctgca tgagccgtcc ctgtatcgct tcggtgctgc gctgcggaac
    12421 aaccggcgcg ggctgacgga tacgggcatc gagaaggcca atgagctgct cgaccagcac
    12481 cttttctccc gtgcccttga tgccattgtg gcgactaaac aaacagatta gaggttaaaa
    12541 aatgagctta tacccgaaag acaaaaatcc cgtgtttcag cctggcggct acggtctgcg
    12601 ctatgacggt caggccgagg ccattgcgcg tgccggtgag ccaccgatca actacgcccc
    12661 gggtcaggag ctgtcccgtg ctgctcctgc gctgcc
//
