LOCUS       NZ_CP069661            33303 bp    DNA     circular CON 18-JUN-2023
DEFINITION  Escherichia coli O89m:H10 strain MIN12 plasmid pMUB-MIN12-MCR,
            complete sequence.
ACCESSION   NZ_CP069661
VERSION     NZ_CP069661.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN17831485
            Assembly: GCF_017570605.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli O89m:H10
  ORGANISM  Escherichia coli O89m:H10
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 33303)
  AUTHORS   Majewski,P.
  TITLE     Plasmid mediated mcr-1.1 colistin-resistance in clinical
            extraintestinal Escherichia coli strains isolated in Poland
  JOURNAL   Front Microbiol (2021) In press
REFERENCE   2  (bases 1 to 33303)
  AUTHORS   Majewski,P.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2021) Department of Microbiological Diagnostics
            and Infectious Immunology, Medical University of Bialystok,
            Waszyngtona 15A, Bialystok 15-269, Poland
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: Unicycler hybrid assembly v. v0.4.8-beta
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 484x
            Sequencing Technology :: Illumina MiSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 06/18/2023 02:09:43
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,209
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,805
            CDSs (with protein)               :: 4,805
            Genes (RNA)                       :: 122
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 14
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 89 of 282
            Pseudo Genes (incomplete)         :: 204 of 282
            Pseudo Genes (internal stop)      :: 44 of 282
            Pseudo Genes (multiple problems)  :: 55 of 282
            Pseudo Genes (short protein)      :: 4 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP069661.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..33303
                     /organism="Escherichia coli O89m:H10"
                     /mol_type="genomic DNA"
                     /strain="MIN12"
                     /serotype="O89m:H10"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:2810405"
                     /plasmid="pMUB-MIN12-MCR"
                     /geo_loc_name="Poland: Bialystok"
                     /lat_lon="53.12523570 N 23.15905989 E"
                     /collection_date="2018-05-05"
                     /genotype="ST-10"
     gene            join(32623..33303,1..228)
                     /gene="virB9"
                     /locus_tag="JSU14_RS25330"
                     /old_locus_tag="JSU14_25370"
     CDS             join(32623..33303,1..228)
                     /gene="virB9"
                     /locus_tag="JSU14_RS25330"
                     /old_locus_tag="JSU14_25370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001299943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-type conjugative transfer protein VirB9"
                     /protein_id="WP_001329500.1"
                     /translation="MMKKLFIATCLLLPGLVYSAATPLPSGFDARMQTVSYNGANTTVI
                     RSKTGFLTSVVFDEGEAVISAKAGFPAGWEITTDDNVVYINPRPVVQEQEGDEGEKLKK
                     VFQPTEKEWDTNLFVRTTKRIYSLDLILLSEEKQAQPAYVVQFRYPSEIAKKNAEEVRL
                     AKEKQEKLRQKKLISESFEKADAPKNWNYFMRVNEKYDSRRIAPDFAYDNGIFTFLGFN
                     SGKVFPAPFAVRDGQEQTLAFNVETKGKYKIMVIHNVNDKFVLRYGNSVVGVVNKSFGK
                     VLTDQRNTSSPAVERVEVNND"
                     /name="virB9"
     gene            221..1339
                     /gene="virB10"
                     /locus_tag="JSU14_RS25335"
                     /old_locus_tag="JSU14_25375"
     CDS             221..1339
                     /gene="virB10"
                     /locus_tag="JSU14_RS25335"
                     /old_locus_tag="JSU14_25375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirB10/TraB/TrbI family type IV secretion system
                     protein"
                     /protein_id="WP_000131271.1"
                     /translation="MTDMKKTDDLVDDANQSKLESKPVHDIGDIRNKNNRMRSGFLFGL
                     MAVLVIGIFALKTYKNYFADDQQKASESTGDTSISQVSKIRTGLGQNFDPVENKAIINP
                     GATGSGNTVSDGHKEVPQEGFRKYLSIPVAGQGGSQTGASGSSRTSQTSEPQEERKESK
                     ENVPGKSGMKVTAINLDPDLYIEENRLIPCALTTRFVSDVAGRISCVFTEDVWSANHHT
                     KLLEQGTKAFGRYQTGTLNHGQGRMFVMWEQLRTPDNKRIDMVNTAAAGPLGEAGIDGW
                     IDSHFWERFGGSLMLSMVQDVAAAAADNAPGKDRNVDYTENSRQAMAEMAKVALENSIN
                     IPPTMYKNQGDIITIMVGEDIDFSDIYELKVK"
                     /name="virB10"
     gene            1341..2369
                     /gene="virB11"
                     /locus_tag="JSU14_RS25340"
                     /old_locus_tag="JSU14_25380"
     CDS             1341..2369
                     /gene="virB11"
                     /locus_tag="JSU14_RS25340"
                     /old_locus_tag="JSU14_25380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001401678.1"
                     /GO_component="GO:0043684 - type IV secretion system
                     complex [Evidence IEA]"
                     /GO_process="GO:0044097 - secretion by the type IV
                     secretion system [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-type DNA transfer ATPase VirB11"
                     /protein_id="WP_077249170.1"
                     /translation="MSGVIPVSISNKSLDFYKNQVFQSFLDIEGLTEIAVNRPGEIWTE
                     INGDWTFHNNDSVTFDFCKRFSHTLASFRGDEIGETKPLLSATLESGERVQVVFPPACE
                     RNTISITIRKPSTRQITHKEYVANGFYDYVQSGKKHRTYDDELLDLFRARNIAAFMELA
                     VAAGKTIVFAGATGSGKTTYMKSLIDFIPLNTRLITIEDAEEIKFFIHKNYVHLFYPSE
                     SGSDSGSIITAAKLIKSCLRMKPDRILLAEIKGGDAWDFVKVTGSGHSGSMTSIHAASA
                     KDAIIQMVTKCYQNRECQNLPFDVLRKIIMDSIDIVVHVGRDGVVRHMSDIYYKGAECE
                     NF"
                     /name="virB11"
     gene            2356..4218
                     /gene="taxB"
                     /locus_tag="JSU14_RS25345"
                     /old_locus_tag="JSU14_25385"
     CDS             2356..4218
                     /gene="taxB"
                     /locus_tag="JSU14_RS25345"
                     /old_locus_tag="JSU14_25385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000847106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxosome-coupling protein TaxB"
                     /protein_id="WP_170324155.1"
                     /translation="MKTFNKSQSVFIFAVMLVAAWFAGSFIFFVLYFSQIKNLKPLKAV
                     FRSIDAYRMDIFTDSLTLSVVTTDIRVLAFVALGAGFVVSLIIPVAALIKLNEKKENIF
                     GDARFATINDIRESNSFTLDGDEKDGIIVGIKDKKIIRYVGAAFSAMGAGTRAGKGAGI
                     VITNLMKYWWSVIILDPKRECFNITSLIRKVILGHEVYKFDPFSSVTHRFNPLYYVSMG
                     TSEGFNQLENLSLIIYPYKTDGADAGSYLNKTAGGVFKSYAVALWFMIKNDKAGLKTLD
                     IEPVFSMSKINQLFERAEPEHLLSFMNDIRSELRGKDKTLADIGVAGLKAFIEMEDKTK
                     AQLKSNFLNGLSPFANPNVANATDGNDFDLRQVRKKRMTIYFCISGDNARLAEKITNIF
                     FQLAIQVNLEKMPTDDPEIKHDCLFLLDEFPSIGAVDYIKSKSGLIAGYKLKLLIVYQV
                     GSQLEEIYSYAGSKTLLASAPCKIVYSASDVKDARELSEAMGTRTVTIGSKSKSRSRGG
                     TSRSESESLIERPLVTTNELLTLKFSEEILAMKGENPIRCEKAFYYLNEYFFGDFVKVA
                     PELANIFPRKKGKLVMPPQKVFENEIVAKGYLVVKDVPDLDKAA"
                     /name="taxB"
     gene            4227..4649
                     /locus_tag="JSU14_RS25350"
                     /old_locus_tag="JSU14_25390"
     CDS             4227..4649
                     /locus_tag="JSU14_RS25350"
                     /old_locus_tag="JSU14_25390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001353737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cag pathogenicity island Cag12 family protein"
                     /protein_id="WP_015059903.1"
                     /translation="MMKKLVFLLISILAGCSSPPEPTPVQFEKANEVINPSLPYVPDFH
                     GVIKSDVSGKGWVYEITSLSGVQARTPTFYYALAHADRIVVTTHDAGLWFRIRDLLKME
                     GATAVVEWRNEKSFLPEQVKIVFIKAQNEVKNDWKK"
                     /name="cag pathogenicity island Cag12 family protein"
     gene            4633..4938
                     /locus_tag="JSU14_RS25355"
                     /old_locus_tag="JSU14_25395"
     CDS             4633..4938
                     /locus_tag="JSU14_RS25355"
                     /old_locus_tag="JSU14_25395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001350475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrbM/KikA/MpfK family conjugal transfer protein"
                     /protein_id="WP_001350475.1"
                     /translation="MTGKNRVVLPALLALGLISTNANASDPCASVLCLYGKAVGQGGGS
                     ECRSAEKDFFNILKKKKGSIRWSKTFDARKAFLNQCSTADPAAISKIMSKFGRVRG"
                     /name="TrbM/KikA/MpfK family conjugal transfer protein"
     gene            <5051..5245
                     /locus_tag="JSU14_RS25360"
                     /old_locus_tag="JSU14_25400"
                     /pseudo=""
     CDS             <5051..5245
                     /locus_tag="JSU14_RS25360"
                     /old_locus_tag="JSU14_25400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000517490.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /name="ATP-binding protein"
     gene            5249..5821
                     /locus_tag="JSU14_RS25365"
                     /old_locus_tag="JSU14_25405"
     CDS             5249..5821
                     /locus_tag="JSU14_RS25365"
                     /old_locus_tag="JSU14_25405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012908955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001198960.1"
                     /translation="MQYALFDGFERKFLLDALEFGVLKDWKENPVKELPDIDESAHPFH
                     ICYGGYLLNPGVSDSDISRKIKDQAGFWLAAIDDTRMDCHSIAYYDIHTLPLISCGHQK
                     IVPFAALIKADECIISKIASYSGFAVTAFLRIKEWDIATNILNREGIFAFNGCERRFRV
                     VSKDNWQHTVSEERAIRCAKRLIQCKG"
                     /name="hypothetical protein"
     gene            5826..7979
                     /locus_tag="JSU14_RS25370"
                     /old_locus_tag="JSU14_25410"
     CDS             5826..7979
                     /locus_tag="JSU14_RS25370"
                     /old_locus_tag="JSU14_25410"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247728.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0003916 - DNA topoisomerase activity [Evidence IEA]"
                     /GO_process="GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IA DNA topoisomerase"
                     /protein_id="WP_001235745.1"
                     /translation="MRLFIAEKPAVANDIVKALGGNFTRHDGWFESDNAIVTNCFGHII
                     ESQPPENYNPEYKAWKVETLPLRLYPVKYQPVESAAKQVKTILELIRRGDVTEIVHAGD
                     PDDEGQLLVDEVLEYAGNTKPVKRVLINDNTLPAVKKALANLKDNRDFKGLYLKALARS
                     VADAVYGFSMTRAYTIPAKARGYQGVLSVGRVQTPVLGLIVNRTRANQNHKSSFYYTMT
                     GVFQRGADVIRANWKPGEFAPLTDRKLLDKAWADGTAASLAGKPATVEAAATDDKKTAA
                     PLPFNLVRLQQYMNKKFKMTAQKTLDITQQLREKYKAITYNRSDCSYLSDEQFSEAPQV
                     IDALKSVFPQSLDIDSARKSKAFNSAKVTAHTAIIPTASVPDVNALSTDERNVYLAIAQ
                     HYLVQFMPEKAYQEVSVAIQCGDESFYARARKTTDSGFEAFLGAETTDEGESEDNDDSA
                     FELLCKIRTGETLTTKEVIVNEKKTTPPPLFTEASLLAALVRVADFVTDPTIKKLLKDK
                     DKDKKDEHGGIGTPATRAAILETLKKRNYITLEKGKLIPTDTGYALIDALPGIAVNPDM
                     TALWSEKQAAIENGDLTVEQFINDLYGELTGMISDVDLGEMKIEPAAPAGQFQRLDSPC
                     PSCGKHIVIRPKGYFCTGCEFKIWSEFSGKKITQAQAEKLVKSGKTDLIKGFKKKSGGT
                     YDTVLVLEDKKTGKLGFPARAKK"
                     /name="type IA DNA topoisomerase"
     gene            7976..8182
                     /gene="hha"
                     /locus_tag="JSU14_RS25375"
                     /old_locus_tag="JSU14_25415"
     CDS             7976..8182
                     /gene="hha"
                     /locus_tag="JSU14_RS25375"
                     /old_locus_tag="JSU14_25415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulator Hha"
                     /protein_id="WP_000850859.1"
                     /translation="MKTKQEWLFQLRKCTSRDTLEKVIEINRYKLPLSESEAFYSAADH
                     RRAELVMNKLYDKVPSGVWKYVH"
                     /name="hha"
     gene            8200..8664
                     /locus_tag="JSU14_RS25380"
                     /old_locus_tag="JSU14_25420"
     CDS             8200..8664
                     /locus_tag="JSU14_RS25380"
                     /old_locus_tag="JSU14_25420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001293133.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="H-NS family nucleoid-associated regulatory
                     protein"
                     /protein_id="WP_001293134.1"
                     /translation="MSELTKEDEYGIISRTMMNIRSLRVFAREIDFEQLLEMQEKLNVV
                     IEERREDAEREAAERQELEAKRQQAIEYIISLGLDPESLLAPVTADTVKTRRKAKGGVR
                     KAKYRFKDENGEIREWSGNGKRPLALQKLLDEGHFMEDFLIEKTKPEQAE"
                     /name="H-NS family nucleoid-associated regulatory protein"
     gene            8751..9455
                     /locus_tag="JSU14_RS25385"
                     /old_locus_tag="JSU14_25425"
     CDS             8751..9455
                     /locus_tag="JSU14_RS25385"
                     /old_locus_tag="JSU14_25425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221000.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS26 family transposase"
                     /protein_id="WP_001067855.1"
                     /translation="MNPFKGRHFQRDIILWAVRWYCKYGISYRELQEMLAERGVNVDHS
                     TIYRWVQRYAPEMEKRLRWYWRNPSDLCPWHMDETYVKVNGRWAYLYRAVDSRGRTVDF
                     YLSSRRNSKAAYRFLGKILNNVKKWQIPRFINTDKAPAYGRALALLKREGRCPSDVEHR
                     QIKYRNNVIECDHGKLKRIIGATLGFKSMKTAYATIKGIEVMRALRKGQASAFYYGDPL
                     GEMRLVSRVFEM"
                     /name="IS6-like element IS26 family transposase"
     gene            10238..10333
                     /gene="dqlB"
                     /locus_tag="JSU14_RS26225"
     CDS             10238..10333
                     /gene="dqlB"
                     /locus_tag="JSU14_RS26225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinQ-like type I toxin DqlB"
                     /protein_id="WP_021534057.1"
                     /translation="MINMLIIVLRAVVAVANALIAVLELIRQFID"
                     /name="dqlB"
     gene            10996..11643
                     /locus_tag="JSU14_RS25390"
                     /old_locus_tag="JSU14_25430"
     CDS             10996..11643
                     /locus_tag="JSU14_RS25390"
                     /old_locus_tag="JSU14_25430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001694199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division plane positioning ATPase MipZ"
                     /protein_id="WP_000587671.1"
                     /translation="MIIVIGGDKGGTGKSHLATNLTVCLSQQGKRTGLVETDLNGSTKK
                     WNKRREQAGLPPVALNEAYGDISAKITKMADVAEILIIDTAGYDSTEFRTALKVADIVI
                     VPIDPLAQVEADSLQTVTKIVRDAQKINPRLAAHVLLYKCQPNTFSEQQELRDSLNSHD
                     YWLKPMKSTVSFLRAFVRAMNQGMGVHELKSNVSGASQAKAQIELLLKELEL"
                     /name="division plane positioning ATPase MipZ"
     gene            11718..11987
                     /locus_tag="JSU14_RS25395"
                     /old_locus_tag="JSU14_25435"
     CDS             11718..11987
                     /locus_tag="JSU14_RS25395"
                     /old_locus_tag="JSU14_25435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001086090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001086090.1"
                     /translation="MAKLELPELEQNQEVNATKFVQDAGKRPTENKTKLASFRIPNELL
                     EFIDNESKRLHRPKTKIIKAALLAYQDLDENELINLWFRADRND"
                     /name="hypothetical protein"
     gene            12148..12852
                     /locus_tag="JSU14_RS25400"
                     /old_locus_tag="JSU14_25440"
     CDS             12148..12852
                     /locus_tag="JSU14_RS25400"
                     /old_locus_tag="JSU14_25440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000052343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2806 domain-containing protein"
                     /protein_id="WP_225236207.1"
                     /translation="MSLIDLSLSGLSEPGTKLIEKISDAIGVLYEPTRIRKKAKAEAEA
                     KRTELISRLELEGIEKRAVERFLKRETKRQENIENITMQAAQSLSESDNVSDIDEDWIE
                     AFFRECEDISDEQMQMLWGRILSEEAKSKGSFSRRTLKLLSTISKEEANLITYFGKFVW
                     QANKLTPILFTDENGDTEGITFDKLSVLDSLGVIQQGIGYSLQIINTLKCIFSMGLRND
                     CLIKFLKYFCIA"
                     /name="DUF2806 domain-containing protein"
     gene            12946..14571
                     /locus_tag="JSU14_RS25405"
                     /old_locus_tag="JSU14_25445"
     CDS             12946..14571
                     /locus_tag="JSU14_RS25405"
                     /old_locus_tag="JSU14_25445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_049589868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine--lipid A transferase MCR-1.1"
                     /protein_id="WP_049589868.1"
                     /translation="MMQHTSVWYRRSVSPFVLVASVAVFLTATANLTFFDKISQTYPIA
                     DNLGFVLTIAVVLFGAMLLITTLLSSYRYVLKPVLILLLIMGAVTSYFTDTYGTVYDTT
                     MLQNALQTDQAETKDLLNAAFIMRIIGLGVLPSLLVAFVKVDYPTWGKGLMRRLGLIVA
                     SLALILLPVVAFSSHYASFFRVHKPLRSYVNPIMPIYSVGKLASIEYKKASAPKDTIYH
                     AKDAVQATKPDMRKPRLVVFVVGETARADHVSFNGYERDTFPQLAKIDGVTNFSNVTSC
                     GTSTAYSVPCMFSYLGADEYDVDTAKYQENVLDTLDRLGVSILWRDNNSDSKGVMDKLP
                     KAQFADYKSATNNAICNTNPYNECRDVGMLVGLDDFVAANNGKDMLIMLHQMGNHGPAY
                     FKRYDEKFAKFTPVCEGNELAKCEHQSLINAYDNALLATDDFIAQSIQWLQTHSNAYDV
                     SMLYVSDHGESLGENGVYLHGMPNAFAPKEQRSVPAFFWTDKQTGITPMATDTVLTHDA
                     ITPTLLKLFDVTADKVKDRTAFIR"
                     /name="phosphoethanolamine--lipid A transferase MCR-1.1"
     gene            14643..15365
                     /locus_tag="JSU14_RS25410"
                     /old_locus_tag="JSU14_25450"
     CDS             14643..15365
                     /locus_tag="JSU14_RS25410"
                     /old_locus_tag="JSU14_25450"
                     /inference="COORDINATES: protein motif:HMM:NF028536.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAP2 family protein"
                     /protein_id="WP_039003037.1"
                     /translation="MPYLSNKRLLAEMSIALVMAIVATLTLEHSQIDLMVADWFYLGMG
                     HWMVAKQAFLPDLLLYSGLKKLLMAMLIYLLVATICRAYHEKKGNAITAKWLVPVTKFR
                     VRELAYLVLTLILVPTVVASLKAYTHVVCPVHLTIFDGTLPYLPMLDSMRNTIPDKCFP
                     AAHASSGFALFAFAFAPSLRRRRGAIIIVVMALGWAMGCYKMIIGDHFLSHTVVSMMLA
                     WAMSAGLAWVFFKKGEQV"
                     /name="PAP2 family protein"
     gene            complement(15756..16283)
                     /locus_tag="JSU14_RS25415"
                     /old_locus_tag="JSU14_25455"
     CDS             complement(15756..16283)
                     /locus_tag="JSU14_RS25415"
                     /old_locus_tag="JSU14_25455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001473365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2726 domain-containing protein"
                     /protein_id="WP_000577043.1"
                     /translation="MIFNRNKKRDEQHNSAVVLPSAENVFSDFIKNNPALTTNAFTAIG
                     EVDCFTKVPLLSNNESEFLGILSRNIIPDYSVFPKIRLIEFVRPHGSEEAVKDLIREVQ
                     NITCDFVLKSYDETVLAVIQFGETATVRQQKKKLIIQRVCAMTGISFFEFKNTVDMMGS
                     EDFCQFLRDDEE"
                     /name="DUF2726 domain-containing protein"
     gene            complement(16418..16822)
                     /locus_tag="JSU14_RS25420"
                     /old_locus_tag="JSU14_25460"
     CDS             complement(16418..16822)
                     /locus_tag="JSU14_RS25420"
                     /old_locus_tag="JSU14_25460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012421203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_012421203.1"
                     /translation="MKDNIVDFQRIRDSMNDHSTDVANDAVYSDKKKISRFFYLLTPVR
                     YLLAASLYLVVVIPLGILAGFRFLLQFLGGIAVMGLTIAWYMGADVSSYVVLAAWIALT
                     VCGCAEQIFEWWIESRFAFRLFGIGRTPEE"
                     /name="hypothetical protein"
     gene            complement(16835..17707)
                     /locus_tag="JSU14_RS25425"
                     /old_locus_tag="JSU14_25465"
     CDS             complement(16835..17707)
                     /locus_tag="JSU14_RS25425"
                     /old_locus_tag="JSU14_25465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001398251.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_001398251.1"
                     /translation="MRMTTNKTSLSRLTKVRHRNELNSTLSTLPMAAKKVLFLAMCQIN
                     SKNEFDDDHIFYVTVADYIKWVQVKPDAAYLALRDGSNILDTTLLKLKHDEILELSSDL
                     GFKFTKSNVPDSMNLSLTVFSTYYRNEGRVGIKFTKEAKRFLCKLIGEEKRYTTQVLLS
                     VVRLTSVNAASLYQLIRKIYSNNSRANSFEMTIDELKDELNLYTIGAGGVKDYKYPDYP
                     AFKRDVLNKSVKEIMKHTEVKNLSFVVSEKIGRKVYKLKFSYTIGYEGDTREDSEFTNM
                     FDKMYPPEN"
                     /name="replication initiation protein"
     gene            19116..19631
                     /locus_tag="JSU14_RS25430"
                     /old_locus_tag="JSU14_25470"
     CDS             19116..19631
                     /locus_tag="JSU14_RS25430"
                     /old_locus_tag="JSU14_25470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025391.1"
                     /GO_process="GO:0006457 - protein folding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="WP_001025395.1"
                     /translation="MNIQEALNVFGLSGELTEKDIKAAYRKAALKYHPDRNPLGAELMK
                     AVNAAFDVLMANIDKINQFQSTDEHARYNYGDDLEKVLNVLSGLSGLVFEVIGNWVWIS
                     GETITHKETLKEIGCKWAAKKKQWFYRPDEHKSYWNREEHTIEEIRAKYGTTGQRRATG
                     WQRVETRA"
                     /name="J domain-containing protein"
     gene            19736..20278
                     /locus_tag="JSU14_RS25435"
                     /old_locus_tag="JSU14_25475"
     CDS             19736..20278
                     /locus_tag="JSU14_RS25435"
                     /old_locus_tag="JSU14_25475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001401654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001083906.1"
                     /translation="MNSFFEQYHPVFEVVCRILGNGWRVNKLDDCSSRIKLTSPQFKNY
                     SVHIRMEKDRFSVVGSVDSRSWRSPHHVCTLSRKRNPVDIAADIERKILVNASQEVLQA
                     IEYEKHQVEKKDEILILKGMLSQLVQLESWYGALTGFKAENGLNGKVTEQGDSYDLQIR
                     GLSIDQLVKITGYLKQL"
                     /name="hypothetical protein"
     gene            20275..20526
                     /locus_tag="JSU14_RS25440"
                     /old_locus_tag="JSU14_25480"
     CDS             20275..20526
                     /locus_tag="JSU14_RS25440"
                     /old_locus_tag="JSU14_25480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001565860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001230707.1"
                     /translation="MRKNKGDVTYFLEKEGDNYRLTKRIKARTNVKIGNKTTKITLYDA
                     VLNENELQHIDFTCAGLREDDETPVKNLIKEFMLNETR"
                     /name="hypothetical protein"
     gene            20513..20740
                     /locus_tag="JSU14_RS25445"
                     /old_locus_tag="JSU14_25485"
     CDS             20513..20740
                     /locus_tag="JSU14_RS25445"
                     /old_locus_tag="JSU14_25485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000801440.1"
                     /translation="MKPDNTPENVKKLRLKAGLTQKECSHIYGVGLRTWQKKEEVNTQN
                     SQSLSLVEFEFLLLLAGEHPEYVLCKRESK"
                     /name="hypothetical protein"
     gene            21194..21493
                     /locus_tag="JSU14_RS25450"
                     /old_locus_tag="JSU14_25490"
     CDS             21194..21493
                     /locus_tag="JSU14_RS25450"
                     /old_locus_tag="JSU14_25490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000673428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000650309.1"
                     /translation="MKCSSVFTSTTNHVFTFERVTLCTIILMHKDTGQQYVVIFTDNNK
                     IRDYKAGIVPQFGELKQSDVDLVLFYRDEYEKYFDSLKDGDECLSFKDFIECLC"
                     /name="hypothetical protein"
     gene            21584..21934
                     /locus_tag="JSU14_RS25455"
                     /old_locus_tag="JSU14_25495"
     CDS             21584..21934
                     /locus_tag="JSU14_RS25455"
                     /old_locus_tag="JSU14_25495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000854261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000854259.1"
                     /translation="MKTLKQAAMQFASDLRNNRCFKAAYNDALLELDREDLNAIVQCTF
                     LFEPKRTLAELDKLIKKIEVEEDYEFCVSLDKGKGLHEVRTFKSEREALDAYRILTLYG
                     YTVTIEKKEGND"
                     /name="hypothetical protein"
     gene            22239..22487
                     /locus_tag="JSU14_RS25460"
                     /old_locus_tag="JSU14_25500"
     CDS             22239..22487
                     /locus_tag="JSU14_RS25460"
                     /old_locus_tag="JSU14_25500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005653200.1"
                     /GO_function="GO:0003729 - mRNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family toxin"
                     /protein_id="WP_000520549.1"
                     /translation="MGKTDKLLAKFLNSKKTFEWDELVVLFSSLGYVKKEMQGSRVRFF
                     NAEINHTILMHRPHPESYIKGGTLKAIKQNLKEAGLL"
                     /name="type II toxin-antitoxin system HicA family toxin"
     gene            22484..22831
                     /locus_tag="JSU14_RS25465"
                     /old_locus_tag="JSU14_25505"
     CDS             22484..22831
                     /locus_tag="JSU14_RS25465"
                     /old_locus_tag="JSU14_25505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000681609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="WP_000681613.1"
                     /translation="MKHLKYKGYLGTVEPDFENNVLYGKLAFIRDLVTYEASTLAELEQ
                     EFKTSVDLYLQSCVEDGKEPDTPFKGVFNVRLDPELHRRVAEMAMEEDLSLNAFVNKAL
                     EKEVSNHRAGA"
                     /name="type II toxin-antitoxin system HicB family
                     antitoxin"
     gene            23192..23695
                     /gene="taxA"
                     /locus_tag="JSU14_RS25470"
                     /old_locus_tag="JSU14_25510"
     CDS             23192..23695
                     /gene="taxA"
                     /locus_tag="JSU14_RS25470"
                     /old_locus_tag="JSU14_25510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000781813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxosome-coupling protein TaxA"
                     /protein_id="WP_000781813.1"
                     /translation="MKMIAFRLSDDELKFAEHNAILSGFTSINAFAKHNVLNIETKPVN
                     IPVNNEPAKLVSVRLYPHEIELVKRNAALHGMSMSREIAIRVRQSLLKSEVCLYPDEVK
                     ELKKLSTAVDRVGRNIHFIIKGERFCTVNDPDFRKDVIEVIELCKQIDSKLETLTKSVV
                     NRFG"
                     /name="taxA"
     gene            23699..24928
                     /gene="taxC"
                     /locus_tag="JSU14_RS25475"
                     /old_locus_tag="JSU14_25515"
     CDS             23699..24928
                     /gene="taxC"
                     /locus_tag="JSU14_RS25475"
                     /old_locus_tag="JSU14_25515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001329504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA relaxase/nickase TaxC"
                     /protein_id="WP_001329504.1"
                     /translation="MGVYVEQEYRIKRAKGTGRDPKSPKLSGRHIHASKSAFNHKVRHG
                     ADKKAYTWTPTKEVTFKITGSGKTAAGIKNGIDYITRNGELEAYCYDGKGSEQTGKGED
                     FNREFTSTLSKGNDYSRTYRGENIDHVKNMVFSPPPEAGVSREDALKATVEFLKETYPN
                     HAFVAVYHDDKEDHPHVHVNIKLRDEETGRRLRLTKSETRKFRNGFHRKLKGMGYDVTA
                     TWKKDPERKREIERLQAENPKRLRNVYKVVDFGETSYQNKAGEKRTPFLTYETLKGGKQ
                     VTIWGKDLKNHFESEKLQPGTLIKVKKLAPTLVRSPMFNDDGTVAGYRETHRNNWQIEN
                     IALERNRERQVHERETRQPEESDVKKQLSRKHEQGHNIGFALEHGFTKDSEEHKKLRIQ
                     QERNWKGLGF"
                     /name="taxC"
     gene            25005..25298
                     /locus_tag="JSU14_RS25480"
                     /old_locus_tag="JSU14_25520"
     CDS             25005..25298
                     /locus_tag="JSU14_RS25480"
                     /old_locus_tag="JSU14_25520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000134733.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0003700 - DNA-binding transcription factor activity
                     [Evidence IEA]; GO:0016987 - sigma factor activity
                     [Evidence IEA]"
                     /GO_process="GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]; GO:0006355 - regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="WP_060598542.1"
                     /translation="MTEHDAICISGLHQIFSDEEHLSEQQKDIILMYAYGYTLNEIADF
                     KGLKPSTVRKYLDSVRAELGGVSLAGIRTLVLIRTNALLVSSLSRISERGNL"
                     /name="sigma factor-like helix-turn-helix DNA-binding
                     protein"
     gene            25295..25510
                     /locus_tag="JSU14_RS25485"
                     /old_locus_tag="JSU14_25525"
     CDS             25295..25510
                     /locus_tag="JSU14_RS25485"
                     /old_locus_tag="JSU14_25525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000760375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EexN family lipoprotein"
                     /protein_id="WP_000760375.1"
                     /translation="MKKYLLFALPFFVVGCSEEVKSVDWWGQHLTEAKQKQAECEKSGS
                     DSQNCKNVKQALFIQSQKDAPVPTFD"
                     /name="EexN family lipoprotein"
     gene            25866..26438
                     /locus_tag="JSU14_RS25490"
                     /old_locus_tag="JSU14_25530"
     CDS             25866..26438
                     /locus_tag="JSU14_RS25490"
                     /old_locus_tag="JSU14_25530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001690148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing protein"
                     /protein_id="WP_227537772.1"
                     /translation="MASLFPALLLCAASVHPDTINDIARVESGFNPYAVAEIIPERERG
                     SYGKSVISHMPKSKEEALTVIREIENRKRRYSVGLMQITSSNFSFYSTSAEKLLDSCEN
                     LSVFEKIIVDCYKRGRSLENALSCYYTGNFSNGKRKEKEFNNTSYVERIGYTGNEKNML
                     YQVQEATGENSEKIVHTMRQLSGPKPF"
                     /name="lytic transglycosylase domain-containing protein"
     gene            26499..26825
                     /locus_tag="JSU14_RS25495"
                     /old_locus_tag="JSU14_25535"
     CDS             26499..26825
                     /locus_tag="JSU14_RS25495"
                     /old_locus_tag="JSU14_25535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000008644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrbC/VirB2 family protein"
                     /protein_id="WP_000008647.1"
                     /translation="MSFKKSVCNRISSSVTAVMQRKNKVVMAVAGATVASPAFADGFSK
                     AETLLEKVKTGLSGLSLVTVTIACLWVGYKVLFGGSTIRECSPIIIGAIVIASAAEVAS
                     MMVN"
                     /name="TrbC/VirB2 family protein"
     gene            26844..29591
                     /locus_tag="JSU14_RS25500"
                     /old_locus_tag="JSU14_25540"
     CDS             26844..29591
                     /locus_tag="JSU14_RS25500"
                     /old_locus_tag="JSU14_25540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirB4 family type IV secretion/conjugal transfer
                     ATPase"
                     /protein_id="WP_000105985.1"
                     /translation="MSTLYKAMTRPAMYVGVPVVPLTVVAGALFLAGVYISKLIWLAIP
                     VAVFVLRMITKQDDHIFNLYFLKLKMLGNSVCNRFFGARAFLSGQYEAVEIDEFVNAMK
                     LNERITTGKYIPYSSHVDKNIVKTKNGDYVATWQLMGINFESISAEMLETIDSQVATLV
                     RSFSGLPVSFYNHSCRASFYDAFTTKSGNKYADIISDCYYGSMKKNKFKGNTLYFTLIY
                     RPDGRVEKLEKRKKSIKEKKDDINIHVKRMNEMINTFSGALDKFTCKLLGMYEENGKVF
                     SSQLSFYNYLLTGKLQKIRVTDSPVYNVLGGVDVFFNHDTGQICRIDGNKFFRSIEIKD
                     FCSETASGVFDVLQYSDADYIITHSYTSMSKSEALSTIKRAEKQLKSTEDDAVTQLQEL
                     EKAKNDIVSGDISFGYYHFTLMVMADSIRELDESVSKITADFTDLGIIPALSTMSLPAA
                     YFAQLPAVFHLRPRLSPVSNVNFVELASFHNFYQGKRDKNCWTEAVAILKTPSKQAYYL
                     NLHNSVLFKDETGEKNLANTKVIGTAGSGKTMFLSYLACSLQKYNNPETFADSAKNKKL
                     TCVFLDKDRGAELCIRMLGGEYYTVKSGEPTGWNPFALEATKRNRIFVKQLMEILCTRN
                     GERLSTRERLLISESVDAVMDFPPGEMREYGITRMLEHLMQRDDRDEQENGIILRLSQW
                     ANGQAHGWVFDNAKDTFNIQHVNNFGIDGTEFLDDPMVCAPITFYLLYRITQLLDGRRL
                     VIFLDEFWKWLQDEAFSDFVYNKLKTIRKLNGLVIPATQSPDEILKNKISRAVVEVCST
                     SIYLANPDADYNDYVEGLKLTPEEFNIVKNLDPMSRQFLIKKSSLKKGDGKSFSALATL
                     DLSGLGGYLKILSASADNLEIFESIYHEGMEPDDWVPEYLERAI"
                     /name="VirB4 family type IV secretion/conjugal transfer
                     ATPase"
     gene            29603..30319
                     /locus_tag="JSU14_RS25505"
                     /old_locus_tag="JSU14_25545"
     CDS             29603..30319
                     /locus_tag="JSU14_RS25505"
                     /old_locus_tag="JSU14_25545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein"
                     /protein_id="WP_000832283.1"
                     /translation="MKRVKTLMLISLLTASFYSSAGIPVAIDASPEWAVEAARWTERLK
                     QWSETAQHYQSQIQAYKDQLATATGIRNIAAFTNELSNLQSELTNIYKQGNSYISDFTS
                     NPEGALSSQAKNLFSKYGAFDMCNTGYERNDNLCKARIVSTAASIEQGNEINKQLSSAM
                     SQIQSLSSRIEASKDIKESQDLANALQAQSLKMQAIKMQYDVWNNKNKADHEMLVTQEQ
                     EAFIKQQKEAEPLTFD"
                     /name="type IV secretion system protein"
     gene            30331..31425
                     /locus_tag="JSU14_RS25510"
                     /old_locus_tag="JSU14_25550"
     CDS             30331..31425
                     /locus_tag="JSU14_RS25510"
                     /old_locus_tag="JSU14_25550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001677777.1"
                     /GO_process="GO:0030255 - protein secretion by the type IV
                     secretion system [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein"
                     /protein_id="WP_000046889.1"
                     /translation="MAQGFFVKYNSTVMDSVDKISSSYQTQFANDIMSLATVSVTLYVL
                     WKGYQILASKTQTPLQDLVWDLSKFAIIIMFITNADGYLTAATDALQGMKDGFSGGVSV
                     WQTLDNLWKSTQNLGAEIYSLDKSTYVKDQGVVGQFLIWTGSLILMAVSVVVFLTADVT
                     MKLLIITAPIFIFCLMFGFIRSMFNNWLQSLFSSILTVLFASLVIRIAMDFQGMILSHA
                     IRAAQTGNVNLVSTGAMGFMAGFLGALLVLIAKGFAVQLAGAGVEGAVQGAAMMGLGAA
                     GMATGKSLMLGGRAGLGFGLGMAGRTGMNSLSGKAGNLMGRGARTAAEWAGEKGYQALA
                     PTGALGMKARRLASLEKARARNAA"
                     /name="type IV secretion system protein"
     gene            31940..32626
                     /locus_tag="JSU14_RS25515"
                     /old_locus_tag="JSU14_25555"
     CDS             31940..32626
                     /locus_tag="JSU14_RS25515"
                     /old_locus_tag="JSU14_25555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001290765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein"
                     /protein_id="WP_001290765.1"
                     /translation="MSEKIEKKIETAKSFERQLYAENERSKKNAWRVAVAASLLSLCLG
                     AAICVLVPLKEKELAIVAVGEKTGRTELITQVKQEKILQSEALGRYFVNTYITLREGYN
                     YPSLQYDYETVQLYSSNTVKDDYLRLYNSDMAPDKIYHNNGSYVAVEVISNIISDATAP
                     DKLASVRFKKTTRNFTTGQVSVSYWTARVTYRFEPEKSVKSSSRELNPLGFTVTSYQTD
                     REVRGE"
                     /name="type IV secretion system protein"
     ARG             12947..14568
                     /name="mcr-1.1"
                     /gene="mcr-1.1"
                     /gene_name="phosphoethanolamine--lipid A transferase
                     MCR-1.1"
                     /tool="amrfinderplus"
                     /drug_class="COLISTIN"
                     /identity="100.0"
                     /coverage="100.0"
     Typing          complement(16836..17707)
                     /name="IncX4"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          23700..24925
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.511"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          2..225
                     /name="MPF_T"
                     /typing="mate-pair-formation"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="1.38e-39"
     Typing          2357..4215
                     /name="MPF_T"
                     /typing="mate-pair-formation"
                     /tool="MOB-suite"
                     /identity="98.71"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          30332..31398
                     /name="MPF_T"
                     /typing="mate-pair-formation"
                     /tool="MOB-suite"
                     /identity="99.719"
                     /coverage="99.0"
                     /evalue="2.69e-172"
     Typing          31941..32623
                     /name="MPF_T"
                     /typing="mate-pair-formation"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="1.4399999999999998e-137"
ORIGIN
        1 ccagctccat ttgctgtaag agatggtcag gaacagacac ttgcttttaa tgtggaaaca
       61 aaaggcaagt ataaaattat ggttatccac aacgttaatg acaaatttgt tttacgctat
      121 ggaaacagcg ttgttggtgt tgtgaataaa tctttcggaa aagtattaac cgaccagagg
      181 aatacatctt ctcctgcggt agaacgtgtt gaggtgaata atgactgata tgaaaaaaac
      241 agatgattta gttgatgatg cgaatcagtc aaagctggaa agtaaacccg ttcatgatat
      301 cggtgatatc aggaacaaaa ataatcgtat gcgttctggc tttctgtttg gtctgatggc
      361 tgtattagtt attggtattt ttgcgctgaa aacatataaa aattattttg cagatgacca
      421 gcagaaagca tcagaatcaa caggcgatac cagcatttct caggtaagta aaatccgtac
      481 tggtcttggt cagaattttg accctgttga gaacaaagca attatcaatc caggcgctac
      541 tggttctggt aatactgttt ctgacggtca taaagaagtg ccacaggagg ggttcaggaa
      601 atatttgtca atacctgttg ctggtcaggg agggagtcag actggcgcga gtggcagcag
      661 caggacatcg cagacatcag agccacagga agaaaggaaa gaatcaaaag aaaatgtgcc
      721 tggtaaatca ggaatgaaag taactgcgat taatctcgat cctgatctct atattgagga
      781 aaacaggctt ataccgtgtg ctttaaccac acgctttgtt tctgatgttg caggtcgtat
      841 cagttgtgtt tttacagaag atgtatggag tgctaatcac cacacaaaat tactggaaca
      901 gggaactaag gcttttggtc gttaccagac cggaacgctg aatcatggac agggcagaat
      961 gttcgttatg tgggagcagt tgcggacgcc tgataataaa cgaatcgata tggtaaatac
     1021 agcagcagct ggtcccctgg gagaagcggg cattgatgga tggatcgatt ctcacttctg
     1081 ggagcgtttt ggtggttctc ttatgctcag tatggttcag gatgtagcgg cggcggcggc
     1141 ggataatgca cctggtaaag atcgtaatgt ggattatacc gaaaactcac gacaggcaat
     1201 ggctgaaatg gctaaagtcg cacttgagaa ttcgataaat attcctccaa caatgtataa
     1261 aaaccagggt gacataatca ctattatggt cggggaagac attgattttt ctgatatcta
     1321 tgagttaaag gtaaaatagt atgagtggcg ttattcctgt cagcattagc aataaatcgc
     1381 tcgattttta taaaaatcaa gtattccagt cttttcttga tattgaaggg ctaacagaaa
     1441 ttgctgtcaa ccgtcctggt gaaatctgga cggaaattaa tggtgactgg acattccata
     1501 ataatgacag tgtgaccttt gatttttgta aacgtttttc tcatacgctg gcatcatttc
     1561 ggggagatga aatcggcgag accaagccat tactttcagc aacactggaa tcaggagaac
     1621 gtgtacaggt cgtttttcct cctgcatgtg agagaaatac tatttcaatc actatacgta
     1681 aaccatcgac acggcagata acacataaag aatatgtggc gaacggtttt tatgattatg
     1741 tccagtcagg aaaaaaacac aggacatacg atgatgagct tcttgattta ttcagagcga
     1801 gaaatatcgc tgcttttatg gagcttgccg tagcagcagg gaaaaccatt gtatttgccg
     1861 gagcaacggg ttccggtaaa acaacttata tgaagagtct tatcgatttc ataccattga
     1921 atacacggct gattacgatt gaagatgcag aggaaatcaa attttttatc cataaaaatt
     1981 atgtgcatct tttttaccct tcggaatccg gcagtgattc cggctctatc ataacagcag
     2041 cgaaattaat caaatcctgt ttgaggatga aacctgacag aattttactg gcggaaatta
     2101 aaggtggcga tgcctgggat tttgtgaaag ttactggttc tggtcacagt ggaagcatga
     2161 cgtccattca cgcggcctcg gcaaaagatg ccatcattca gatggtgaca aaatgttatc
     2221 agaacagaga atgtcagaat cttccttttg atgttcttcg taaaatcatt atggacagta
     2281 ttgatattgt tgttcatgta ggcagggatg gtgttgtcag gcatatgagc gacatctatt
     2341 acaaaggggc tgaatgtgaa aacttttaat aagtcgcaat ctgtttttat attcgctgtt
     2401 atgcttgttg cggcatggtt tgcaggttca tttattttct ttgtgcttta cttcagccag
     2461 ataaagaatc tgaaaccatt gaaggctgtg ttcagatcca ttgatgcgta caggatggat
     2521 atttttacag acagccttac tttatctgtt gtgactacgg atatcagagt tttagccttt
     2581 gttgcacttg gagcaggatt tgtcgtgtct cttatcatcc ctgttgcggc gttgataaaa
     2641 ctgaacgaga agaaagaaaa tattttcggt gatgcgcgat ttgcaacaat taatgatatt
     2701 cgtgagtcta acagtttcac gcttgacggt gacgaaaaag acgggattat cgttggtata
     2761 aaagacaaga aaatcatacg ctatgtggga gcagcattca gtgctatggg agccggaaca
     2821 agggctggta aaggtgctgg tatagtcatc actaatctta tgaagtactg gtggagtgtt
     2881 attattctcg atccaaaaag ggagtgtttt aatatcacca gtcttatcag gaaggtgatt
     2941 ttaggtcatg aggtttataa atttgatccc tttagttcgg taacgcaccg ctttaaccct
     3001 ttgtattatg tcagcatggg aacgagtgaa ggttttaatc agcttgaaaa tctttcgttg
     3061 attatttacc cctataagac agatggtgcg gatgctggta gctatcttaa taagacggcg
     3121 ggtggtgttt ttaaatctta tgctgtcgct ttatggttta tgattaagaa tgataaggct
     3181 ggactaaaaa cgcttgatat tgagcctgtc ttctcaatgt ctaaaattaa tcagcttttt
     3241 gagcgtgcag aacccgaaca tcttctttca ttcatgaatg atattcgttc agaattaagg
     3301 gggaaagaca aaacgcttgc tgatattggt gtcgctggcc ttaaggcatt cattgagatg
     3361 gaggataaaa caaaagcaca actcaaaagc aactttctta atggattatc accgtttgct
     3421 aaccccaatg tggcaaatgc tactgatgga aatgattttg atttgcggca ggtgcggaaa
     3481 aagcggatga caatttattt ctgtatttct ggtgataatg ccaggttggc tgaaaaaata
     3541 acaaatattt tctttcagct tgctatacag gtgaatcttg aaaagatgcc aacggatgat
     3601 ccggaaataa agcatgactg cctttttcta ctggatgaat tcccgtcaat tggtgctgta
     3661 gattacatca agagtaaatc tggtctcatt gctggttaca agctgaaatt gttgattgtt
     3721 taccaggtcg gctctcagct tgaggaaatc tacagttatg caggcagtaa aacattactg
     3781 gcaagtgcac cctgcaaaat tgtttattcc gcttctgatg ttaaagatgc gcgggagtta
     3841 tcagaagcga tggggacacg aacagtaact atcggttcga aaagcaaaag tcgtagtcgt
     3901 ggaggaacat cgagatctga atcagaaagc cttattgagc gtccactggt aacaacaaat
     3961 gaactactta cactgaaatt tagtgaagaa atcctggcaa tgaagggaga aaacccgatt
     4021 cgttgtgaaa aggcattcta ctatctcaat gagtatttct ttggtgactt tgtgaaggtt
     4081 gctccggaac ttgcaaacat tttccccagg aagaaaggaa agctggttat gcctccacag
     4141 aaagtatttg aaaatgaaat cgtcgcaaaa gggtatctgg tagtaaaaga tgttcctgat
     4201 ctcgataaag cggcataaag gagaacatga tgaagaagtt agtgttttta ttgatttcca
     4261 ttcttgctgg ttgttcatca ccgccagagc caacacccgt gcagtttgaa aaggcaaatg
     4321 aagtgatcaa tccctcattg ccatatgtcc ccgattttca cggtgtcata aagtcagacg
     4381 ttagcggtaa aggatgggtt tatgagatca cttccttatc cggagtacag gccagaacgc
     4441 cgacgtttta ttacgctctg gctcatgcgg acaggattgt agtgacaaca catgatgctg
     4501 ggctgtggtt tcgaatcaga gatctgttga agatggaagg ggcaacagca gtcgtggaat
     4561 ggcgaaacga gaaaagtttt ttacctgaac aggtaaaaat agttttcatt aaagcacaaa
     4621 atgaggtgaa aaatgactgg aaaaaataga gtggttttac cagccttgct ggcgttagga
     4681 ttaatatcaa caaatgctaa tgcatctgac ccttgcgctt cggtactttg cttatatgga
     4741 aaagcagtcg ggcaaggtgg agggagtgaa tgcagaagtg ctgaaaagga tttttttaat
     4801 attctgaaaa agaaaaaagg aagcattcgc tggagtaaaa cttttgatgc ccgtaaagca
     4861 tttctgaatc agtgttcaac ggctgatcct gctgctatat caaaaatcat gagtaagttt
     4921 ggtcgtgtca gaggttaatt aaaacctgat aaccgcgcca acgggtatca gggtactgca
     4981 agtatcacat ttcgcgccaa cgaaatgtga tgattagtta caatcactga ggttagaaat
     5041 gaaagtaacc aaatctatta tagctatttt attgtgctta tctgctggta gtgcataagt
     5101 ggatgtaata aaaaatgctc gtgccttttt gaaaggaacc ctcgatctga gcgttcagga
     5161 agcagatgaa gatatagagg aaagggaaga actttataaa aagaacgggg cacaacctga
     5221 ctatttaagt tacctggagg attaacacat gcaatatgca ttgtttgatg gatttgaacg
     5281 aaagtttttg ctggatgctc ttgaattcgg tgttctgaag gactggaaag aaaatccggt
     5341 aaaagaactt cctgatattg atgaatctgc tcaccccttc catatctgtt atggtggata
     5401 tttattaaac cctggtgttt cagattcaga tattagcaga aaaataaaag accaggcagg
     5461 attctggctg gcagctattg atgatacccg catggattgt cattcaatag cttattatga
     5521 tattcacaca ctccctctga tttcgtgtgg tcatcaaaag atagttcctt ttgccgcgtt
     5581 aataaaagct gatgaatgca tcatttcaaa aattgcttcg tattctggtt ttgctgtaac
     5641 agcctttttg agaattaaag agtgggatat tgcaactaat atacttaacc gtgagggaat
     5701 ttttgccttt aatggctgtg agcgcagatt cagagtagta agtaaagata actggcaaca
     5761 tacagtatcg gaagaacgcg ctatccgttg tgccaaaaga ttaattcaat gtaaaggata
     5821 acaaaatgag actttttatc gcagaaaaac ccgcagtagc aaatgatatt gttaaggcac
     5881 ttggtggcaa ttttacccgc catgatggct ggtttgaaag tgataacgcc attgtgacta
     5941 actgttttgg tcatattatc gaatcacaac cgccggaaaa ctataatcct gaatacaaag
     6001 cctggaaggt tgaaacgctt cctttacgtc tttatcccgt gaagtatcag cctgttgaaa
     6061 gtgcagcaaa acaggttaaa acgattctcg aacttatcag acgtggagac gtgactgaaa
     6121 ttgttcacgc tggcgatcct gatgatgagg gacagctact ggttgatgaa gtcctggaat
     6181 atgcaggcaa cacaaaaccc gtaaagcgcg ttctgattaa cgacaacacg cttccggcag
     6241 tgaaaaaggc actggcaaat cttaaagaca atcgtgattt caaaggactt taccttaagg
     6301 cgttggcgcg gtcagttgcc gatgccgtct atggcttctc tatgacacgt gcgtacacca
     6361 ttccggcaaa agccagagga tatcagggcg ttctgtctgt cgggcgcgtc cagactcccg
     6421 ttcttggcct gattgtgaat cgtacccgtg ctaaccagaa ccataaatcc agtttttact
     6481 acaccatgac cggagtcttt cagcgtggtg ctgatgttat cagggcgaac tggaagccag
     6541 gggaatttgc tccgctgaca gaccgtaaat tacttgataa agcgtgggca gacggaacgg
     6601 ctgcatccct tgcaggaaaa ccggctacag ttgaagcagc agcaactgat gataaaaaaa
     6661 cggctgcgcc gttgccgttt aatctggtca gactccagca atacatgaac aagaagttta
     6721 aaatgacggc acaaaaaacg ctggatatta cgcaacaact acgcgaaaaa tataaagcaa
     6781 ttacgtataa ccgctctgac tgctcatatc tttcagatga acaattcagc gaagcgccgc
     6841 aggttatcga tgccctgaaa tcagtctttc ctcagtcgct ggatattgat tctgcacgta
     6901 aaagcaaagc gtttaacagt gcaaaggtga ctgcgcatac tgcgataatc ccgaccgcca
     6961 gtgtgcctga tgttaacgca ctcagcaccg acgagcgcaa tgtttacctg gcgatcgcac
     7021 aacactatct tgttcagttc atgcctgaaa aagcatacca ggaagtatcg gttgccattc
     7081 agtgtggtga tgagtcgttc tatgcccgtg ccagaaaaac aactgacagc ggatttgagg
     7141 cgtttcttgg cgcggaaacc acagacgaag gtgaatcaga agataatgat gattccgctt
     7201 ttgaactgct ctgtaaaatt cgcacaggag aaacactgac gacaaaagaa gttattgtta
     7261 atgagaagaa aacaacaccg ccgccgttat tcaccgaagc ctccttgctt gctgcgcttg
     7321 ttcgtgtcgc ggattttgtc actgatccaa caattaaaaa attgttgaag gataaggata
     7381 aagacaaaaa agatgaacat ggtggcattg gtacgccagc tacccgcgca gccattctgg
     7441 aaacgctgaa gaaaagaaac tatatcacgc tggaaaaagg gaaacttatt cctactgata
     7501 ccggatatgc gcttattgat gccctgcctg gtatagcggt taatcctgat atgacagcat
     7561 tatggtctga aaagcaggct gccatagaaa atggcgacct gacggttgaa cagtttatta
     7621 atgatctgta cggtgaactg acaggcatga tttctgatgt tgacctgggc gagatgaaga
     7681 ttgaacccgc tgcgccagca gggcagtttc aacgcctgga ctctccctgc ccttcctgtg
     7741 gtaaacatat tgttatcagg ccgaaaggtt atttctgtac cggatgtgaa tttaaaatct
     7801 ggagtgagtt ttctggtaag aaaatcaccc aggcacaggc cgaaaaactg gttaaatcag
     7861 ggaaaaccga tttgattaag ggatttaaaa agaaaagtgg tggaacgtat gatacagttc
     7921 ttgtccttga ggataagaaa acagggaagc tgggttttcc ggcaagggct aagaagtgaa
     7981 aacaaagcag gaatggcttt ttcagttaag aaaatgtaca tcaagagata ctcttgaaaa
     8041 agttattgag attaaccgtt acaaactgcc tttatcagaa tcagaggcat tttattctgc
     8101 cgcagatcat cgccgagcag aactggtgat gaataaactt tatgataagg tgccttccgg
     8161 cgtctggaag tacgtacact aaataagagg attaattata tgagcgaact gactaaagaa
     8221 gatgaatacg gcattatcag ccgtactatg atgaatattc gttcattgcg tgtttttgcc
     8281 cgtgagattg attttgagca gttgctcgaa atgcaggaaa agctcaacgt tgttattgaa
     8341 gaacgtcgtg aagatgctga acgtgaagcg gctgaacgcc aggaacttga agccaaacgt
     8401 cagcaggcca ttgaatacat catatcttta ggtcttgatc ccgaatcact ccttgctcct
     8461 gtaacagcag atacagtaaa aaccaggcgg aaagccaaag gcggagtaag aaaggctaaa
     8521 taccgcttta aggatgaaaa tggcgagatc agagaatggt ctggtaatgg caagcgtccg
     8581 cttgctttac agaagctgct tgatgagggg catttcatgg aagatttcct cattgagaaa
     8641 actaagccgg aacaggcaga ataacccgcc aggtatacat cacacagggc actgttgcaa
     8701 atagtcggtg gtgataaact tatcatcccc ttttgctgat ggagctgcac atgaacccat
     8761 tcaaaggccg gcattttcag cgtgacatca ttctgtgggc cgtacgctgg tactgcaaat
     8821 acggcatcag ttaccgtgag ctgcaggaga tgctggctga acgcggagtg aatgtcgatc
     8881 actccacgat ttaccgctgg gttcagcgtt atgcgcctga aatggaaaaa cggctgcgct
     8941 ggtactggcg taacccttcc gatctttgcc cgtggcacat ggatgaaacc tacgtgaagg
     9001 tcaatggccg ctgggcgtat ctgtaccggg ccgtcgacag ccggggccgc actgtcgatt
     9061 tttatctctc ctcccgtcgt aacagcaaag ctgcataccg gtttctgggt aaaatcctca
     9121 acaacgtgaa gaagtggcag atcccgcgat tcatcaacac ggataaagcg cccgcctatg
     9181 gtcgcgcgct tgctctgctc aaacgcgaag gccggtgccc gtctgacgtt gaacaccgac
     9241 agattaagta ccggaacaac gtgattgaat gcgatcatgg caaactgaaa cggataatcg
     9301 gcgccacgct gggatttaaa tccatgaaga cggcttacgc caccatcaaa ggtattgagg
     9361 tgatgcgtgc actacgcaaa ggccaggcct cagcatttta ttatggtgat cccctgggcg
     9421 aaatgcgcct ggtaagcaga gtttttgaaa tgtaaggcct ttgaataaga caaaaggctg
     9481 cctcatcgct aactttgcaa cagtgcctca cacagacata cacataggta tgtctgtgtg
     9541 acatacctat gtacagtgcc agatggcgtt gctacgtgaa aaataaccta ccctacccct
     9601 gcttttttcg cagattttac gtagcacacc gttgctatag cattcatcta aaaagctgaa
     9661 tgtccttttc tgtcgctgtt gcattttaat tcactgaaaa atattgagta atgaaaatgc
     9721 cggtaacgcg tcaagctgtt ggccaacgcg acaggcagta ttggaagcaa caaaaaaaat
     9781 gaaagaaaaa tccctcaaat gaaggagaaa aaacggaact acagccgaac gacaggcaca
     9841 aaaaaacccg actggtaatc gggctttttt gcgttttcca gtcttgagtt ggtggctctg
     9901 accggagaag gttactctac acctaacgtt tacattttat tcactatggt gctgtaaagc
     9961 aactaaatgt ggttaaggaa ccacatttag tggtgtaacg ggtgttttgt gtagcgtata
    10021 atcagtcagt tagtcatgta gttggcttgc aatcctgctg ttcccgtatc tgcgggtatg
    10081 aacgatttta aagtcctggc tggataaaca ggtggtgctg tattcggtgc tctggcagga
    10141 ggaggtcact ctacacctaa caatgcgaga gagccgttgt gacaggcagt gtatgagcaa
    10201 gcaacggtga tttcctccag ttttactgga gaaacggatg attaatatgt taatcatcgt
    10261 tctcagagct gtggtcgccg ttgcaaacgc gctgattgca gttctggaac tgatccgcca
    10321 attcatcgat tgatgacaac ggaaacgtaa ctaagggcag gaagtgaacc gcttcctgcc
    10381 ctttaacaaa aaaggaggat ttatggattt tatgagcatt ttggggcgct gtttacttct
    10441 tcctttccct gttgcgatgt ttgtcggttg cctgtttcct ggcatcgatg atcgcttatc
    10501 cggaatgctt atgagtgttg ctgtgggatg tttatcgtgg tatgtcacca gaccgaagaa
    10561 ccgacagaca aaggccgcgt aagtggcctt taattgggat accctttagt tacaacattc
    10621 taatagctca ttccatcctc cgctcatcca gcaaacataa cctccctgcc cctccccact
    10681 acagaaagtg gcgcgccccg cccgtacggg cggaatctaa tcgcttaatt ttgaatgttg
    10741 taactaaaca tactcattgc tgtattgcca caaggtttcg cagcaaggga ggataccgaa
    10801 agtaacgctg tcacttttgc tttcaagtga ccaaccaacg ggtacatcac actgacatac
    10861 aataatgtat ttcttcatga tgtacatcat tgtaattcat tgtgacatac actatagtat
    10921 gtccatgtga catacgcatg cgtacatcac atagaaatac acatgagtaa atcactgtga
    10981 catacaaggg gtactatgat tatcgtaatc ggtggtgata aaggcggtac aggtaaatca
    11041 catctggcaa ccaacttaac tgtttgcctc tcacaacagg gaaagagaac ggggcttgtt
    11101 gaaacagacc tgaatggctc gaccaaaaaa tggaataaac gcagagaaca ggccggatta
    11161 ccgcccgtcg cattaaatga agcctatggc gacatcagtg ccaaaataac aaaaatggca
    11221 gatgtagcag aaatcctcat tatagatact gccggatatg acagcacgga gttcagaact
    11281 gcactaaaag ttgcagacat tgtgattgtc ccgatcgacc cgcttgccca ggtggaagca
    11341 gacagtcttc agactgtgac aaaaattgtc agggatgcgc aaaaaataaa tcccaggctg
    11401 gctgcacatg tgctcctgta taaatgccag ccaaatacgt tcagtgaaca acaagaatta
    11461 agagactctc tcaacagcca tgattactgg cttaaaccaa tgaaaagcac ggtttctttt
    11521 ctccgggcct ttgtaagagc aatgaatcag ggaatggggg ttcacgagtt aaaaagtaat
    11581 gttagcggtg ccagccaggc aaaagcacaa atcgaactct tgctgaaaga actggaactg
    11641 taaatcatag tgacatacac taatgtacat catcatgata tacattagtg tgaaacaatc
    11701 agattagaag gtacatcatg gcaaagctgg aactaccaga actagaacaa aaccaggaag
    11761 tgaacgccac aaaatttgtt caggacgcag gaaaacgccc cacagagaac aaaacaaaac
    11821 tggcttcatt ccgcataccg aatgaattgc ttgaattcat tgataacgaa tcaaaaagac
    11881 tgcatcgtcc caagacaaaa ataatcaaag cagcattact ggcttatcag gatctggacg
    11941 aaaacgaact cattaatttg tggttcagag ctgacagaaa tgattaatca tagcaagtgg
    12001 cccaataaac tctttatcat tttcttttag atacttatct gaatgtacta acttcatcac
    12061 ttttaaatgt agttgtgatt catttttagt tactcttgca ataaaatacc cttgtacagt
    12121 tgttgcacgg gtatttagga gcactagatg agccttatcg atttaagttt atcaggatta
    12181 tcagaaccag gaacaaaact tattgaaaaa ataagtgatg ctattggtgt gctttatgaa
    12241 cctacaagaa tcaggaaaaa agcgaaggct gaagctgaag ctaaacgcac agagctaatt
    12301 tctaggttag agcttgaggg aatagaaaaa cgggcagttg agcgttttct caaacgtgaa
    12361 acgaaacgac aggaaaacat tgaaaatata acaatgcaag cagcacaaag tctatctgag
    12421 agtgataatg tttctgatat tgacgaagat tggatagagg ctttcttcag ggagtgtgag
    12481 gatattagtg acgaacaaat gcaaatgctt tggggaagaa ttttatccga agaagcaaag
    12541 tcaaaaggtt cgtttagtcg tagaacctta aaactattat ccactataag caaagaagaa
    12601 gcgaacctta ttacttattt tggaaagttt gtttggcagg caaacaaact cactccaatt
    12661 ttatttaccg atgagaatgg agatactgaa ggtataactt tcgataaact ttcggtttta
    12721 gactccttag gcgttattca acaaggcatt ggttatagtt tacaaattat aaatactctc
    12781 aagtgtatat tcagtatggg attgcgcaat gattgcctaa taaaatttct gaaatatttc
    12841 tgtatcgcat aattttttat atcagataaa ttgtactgga tttcttaaaa aattgcagta
    12901 taattgccgc aattatcccg ccgtttattt tttgagtagt ttctcatgat gcagcatact
    12961 tctgtgtggt accgacgctc ggtcagtccg tttgttcttg tggcgagtgt tgccgttttc
    13021 ttgaccgcga ccgccaatct tacctttttt gataaaatca gccaaaccta tcccatcgcg
    13081 gacaatctcg gctttgtgct gacgatcgct gtcgtgctct ttggcgcgat gctactgatc
    13141 accacgctgt tatcatcgta tcgctatgtg ctaaagcctg tgttgatttt gctattaatc
    13201 atgggcgcgg tgaccagtta ttttactgac acttatggca cggtctatga tacgaccatg
    13261 ctccaaaatg ccctacagac cgaccaagcc gagaccaagg atctattaaa cgcagcgttt
    13321 atcatgcgta tcattggttt gggtgtgcta ccaagtttgc ttgtggcttt tgttaaggtg
    13381 gattatccga cttggggcaa gggtttgatg cgccgattgg gcttgatcgt ggcaagtctt
    13441 gcgctgattt tactgcctgt ggtggcgttc agcagtcatt atgccagttt ctttcgcgtg
    13501 cataagccgc tgcgtagcta tgtcaatccg atcatgccaa tctactcggt gggtaagctt
    13561 gccagtattg agtataaaaa agccagtgcg ccaaaagata ccatttatca cgccaaagac
    13621 gcggtacaag caaccaagcc tgatatgcgt aagccacgcc tagtggtgtt cgtcgtcggt
    13681 gagacggcac gcgccgatca tgtcagcttc aatggctatg agcgcgatac tttcccacag
    13741 cttgccaaga tcgatggcgt gaccaatttt agcaatgtca catcgtgcgg cacatcgacg
    13801 gcgtattctg tgccgtgtat gttcagctat ctgggcgcgg atgagtatga tgtcgatacc
    13861 gccaaatacc aagaaaatgt gctggatacg ctggatcgct tgggcgtaag tatcttgtgg
    13921 cgtgataata attcggactc aaaaggcgtg atggataagc tgccaaaagc gcaatttgcc
    13981 gattataaat ccgcgaccaa caacgccatc tgcaacacca atccttataa cgaatgccgc
    14041 gatgtcggta tgctcgttgg cttagatgac tttgtcgctg ccaataacgg caaagatatg
    14101 ctgatcatgc tgcaccaaat gggcaatcac gggcctgcgt attttaagcg atatgatgaa
    14161 aagtttgcca aattcacgcc agtgtgtgaa ggtaatgagc ttgccaagtg cgaacatcag
    14221 tccttgatca atgcttatga caatgccttg cttgccaccg atgatttcat cgctcaaagt
    14281 atccagtggc tgcagacgca cagcaatgcc tatgatgtct caatgctgta tgtcagcgat
    14341 catggcgaaa gtctgggtga gaacggtgtc tatctacatg gtatgccaaa tgcctttgca
    14401 ccaaaagaac agcgcagtgt gcctgcattt ttctggacgg ataagcaaac tggcatcacg
    14461 ccaatggcaa ccgataccgt cctgacccat gacgcgatca cgccgacatt attaaagctg
    14521 tttgatgtca ccgcggacaa agtcaaagac cgcaccgcat tcatccgctg atttctccct
    14581 gtattttttc caaacccacc gcacactcca ttcgtattat gggcggtggg gtggggtttg
    14641 ttatgccgta tttatcaaat aaacgcctac ttgctgagat gagtatcgct cttgtcatgg
    14701 cgatcgttgc cacgctgacc cttgagcaca gtcagattga tctgatggtc gctgattggt
    14761 tttatctggg tatggggcat tggatggttg ccaagcaagc ttttttgcca gatttgctac
    14821 tgtattctgg actaaaaaag ctgctgatgg cgatgctgat ctacttgctg gttgcgacca
    14881 tttgccgtgc ttatcatgag aaaaagggca atgctatcac tgccaagtgg cttgtcccag
    14941 tgacaaaatt tcgcgtgcgt gagcttgcgt atctggtgct gactttgatc ctagtgccga
    15001 cagttgtcgc gtcattgaag gcatatactc atgtggtctg ccctgtgcat ttgacgattt
    15061 ttgatggtac gctgccgtat ttgccgatgc ttgatagtat gcgtaacacc attcctgata
    15121 agtgctttcc tgcggcgcat gccagtagcg gatttgcgct gtttgccttt gcgtttgcgc
    15181 caagtttgcg ccgccgtcgt ggtgcgatca tcatcgtggt gatggcattg ggctgggcga
    15241 tgggctgcta taagatgatt attggcgatc attttttgag ccatacggtg gtgtcgatga
    15301 tgcttgcgtg ggcgatgtcg gcagggcttg cgtgggtgtt ttttaagaag ggtgaacaag
    15361 tttaatcgcc tttaaaagta aaaacggtcg tatcggttat tacggaacgg ttttttcagt
    15421 tgagtttcaa agcgatgatt catcaacctg gaaactacca ataggaacgg ctttacttac
    15481 gcctatcggt caagaattga tgaaaatatg cggttccact ccagatattg cgtatttaaa
    15541 taaattcctc aacaaaataa atgttgaagg aagccaggta aaactatcaa ttattaatat
    15601 gtaaagaggt cgagttacct gtctgagttc cactaaccat aattaaaagc gaccgcaaaa
    15661 actgcccagt gaaaactgtg ttgccagtaa tcacaaatgc agcaagtact aataaagagc
    15721 ctgttaagcc cgcaaatatg tgggcttatt tttaattact cttcatcatc ccgcaggaac
    15781 tgacaaaaat cttcgctccc catcatatcg acagtgtttt taaattcgaa aaaagaaatt
    15841 cctgtcatag cacacacgcg ctggataatc agttttttct tttgctgtct gacggttgcg
    15901 gtttcaccaa actggataac agccaggact gtttcatcat atgatttcag gacaaaatca
    15961 caggttatat tctgcacttc acggattaaa tcttttacag cttcctcgct accatgagga
    16021 cgaacaaatt caatgagccg tattttggga aaaacggaat aatcaggtat aatattacgt
    16081 gaaagaatgc ctaaaaactc tgattcatta tttgagagta agggtacttt agtaaaacaa
    16141 tcaacctccc caatggctgt aaaagcattg gttgtcaaag ctggattgtt cttaataaaa
    16201 tcagagaata cattttcagc tgatggcaaa acaacagcag aattatgttg ttcatctcgt
    16261 tttttattac ggttaaatat catttcatat tccttttaaa agagcctgcg gcaggcacag
    16321 gcaaaaacat cgtttttgga caatctgatt caaatgttaa catttgacat cggattgtct
    16381 atcctgcatt gttctttact gtctgtaaga tcggcgctca ttcttccggc gttctcccga
    16441 taccaaaaag acggaacgca aaacggcttt cgatccacca ttcaaaaatc tgctctgcgc
    16501 aaccacaaac agttaaggcg atccatgcag caagaacaac gtaactactg acatctgcac
    16561 ccatgtacca ggcgatagtt aaacccatca ctgcgatacc gcctaaaaac tgcaacaaga
    16621 aacgaaatcc cgcaagaatc cccaatggaa taacaacaac cagataaagg gaagcagcca
    16681 gaaggtatct gacaggtgtc agcagataaa agaacctgga tattttcttt ttgtcagaat
    16741 aaacggcatc atttgcaaca tcggttgaat gatcattcat gctgtccctg attctttgaa
    16801 aatctacgat attatccttc atattgttat gacctcaatt ttcaggtggg tacattttat
    16861 caaacatatt agtaaactct gagtcttccc tggtatcgcc ctcataacca attgtataag
    16921 agaactttaa tttataaact tttctcccaa ttttttctga aactacgaaa cttaagttct
    16981 ttacttccgt atgtttcatg atttctttta ctgatttatt aagaacatct cttttaaaag
    17041 caggataatc tggatactta taatccttca ccccccccgc ccctattgta taaagattca
    17101 attcatcttt aagttcatct attgtcattt caaaactatt cgcgcgagaa ttattactat
    17161 atattttacg aatgagttga tatagtgatg cagcattaac acttgtcagt cgaacaacag
    17221 ataataaaac ttgcgttgta taacgttttt cttcaccaat aagtttacat aaaaatcttt
    17281 tcgcttcttt cgtaaacttg atccccactc taccttcatt cctgtaataa gtagaaaaaa
    17341 cagtcagact caaattcatt gaatcaggta cattactttt agtgaactta aatcccaaat
    17401 cactactcag ttctaatatt tcatcatgtt tcagcttaag aagcgtcgta tctaatatat
    17461 tagagccatc cctcaaagca agataagccg catcaggctt aacctgaacc catttaatgt
    17521 aatcggcaac tgtcacataa aatatgtgat catcatcgaa ttcattttta gagtttatct
    17581 ggcacatggc taaaaataag acttttttag cagccatagg caatgttgac agcgttgaat
    17641 tcaattcatt tcgatgtctc acttttgtta agcgagaaag ggaagtctta tttgtcgtca
    17701 ttctcattgt tccaacactc ttgataatca gactttaaca ccagaaacat gatattacaa
    17761 cacaatcaat atcatgttta accgagtatc ttctcatgtt tcatatctgt gatttatctc
    17821 acaaaggtaa tccatgctca aaatccacac tggattcaag gcagattaac aacagattca
    17881 aaatagattc accagaaaga acaacgaatt agtattctta tgacacattt tgtggatatc
    17941 accgaggaag ctctcatgtt taaccgagga agctctcatg tttaaccgag gaagctctca
    18001 tgtttaaccg aggaagctct catgtttaac cgaggaagtt atcatgttta accgaggaag
    18061 ctctcatgtt tagacaaaaa acacgcttaa aatcaataaa ttagagctac tctaagtttt
    18121 taagttttta agaaaaacaa gaaaagataa ggcttttaat gcgtgcgaat attcaggaac
    18181 tgtggataac tcaacaaaac cagcaacgat gatggttcct taccataaat ctgtcccccg
    18241 atgattatta cccgcaagct aaaacaaaca tcatcaatca ttaaaactca taactttcaa
    18301 taacataaaa tcatcaaaaa aatatttgat gccttatgta tctcaatttt caagaccata
    18361 aatttgtccc tagttaactt taacttattg aaataaatat aatattcgtg cgtactgatc
    18421 ctaatgtcac atcgcaaaaa ttccgcttcc tcgctcactc agtcgctacg ctcctgccgt
    18481 tcggctgcgg cgagcggtat ctcctttctc aaaaccagag agattcagat catatgatcc
    18541 gttatttgat cctttttgga tcttccacta agaagccctg aatcgcgctc tgccgcttcg
    18601 ttcgatacaa ccatgaggaa aaccatgcga aatgaaaaaa atcgcttaaa atgcgattca
    18661 gagcgtttta gggaggttct gttagttctc ctgcattgcg cccgtagttc gctaacgctc
    18721 acacgcgcag tacagcgccc cgccatgctg caatgcacca gggcggggta aactcgcttg
    18781 cgctcgcgct aactgcggca ttctgcggcg caaatcagca taatcatggc ctgctggtca
    18841 gttgggcttt gcagggtgaa gggatggcct gtgcaccctg gtacgttcgt cagcggctgc
    18901 gccgcgcttc ggggcgatgt ggctcctgtg ctgccctggt attttcgttc gccccttcca
    18961 tccgcttacg cggattaaaa aacgtgataa caatcactta tcgagtaaaa aaacgggaga
    19021 aaacttgcaa atacgactta aaggtcgtat tattgtttca tcgaaagggg aatggcccct
    19081 ctcacaatag cccagcaggg caggagatta aaaaaatgaa tatccaggaa gcattaaacg
    19141 tttttggatt atccggcgaa ttaactgaaa aagatatcaa agcagcatac agaaaagccg
    19201 ctttaaaata tcatccagat cgtaacccgt taggggctga actgatgaaa gcggtaaatg
    19261 cagcttttga tgtattgatg gcaaatattg ataaaataaa tcagttccag agcactgatg
    19321 aacatgcacg atataattac ggtgatgacc tggaaaaagt attaaacgtt ctttctggtt
    19381 tatctggtct ggtatttgaa gtgataggta actgggtctg gattagtgga gaaaccatta
    19441 cacataagga aactttaaaa gaaatcgggt gtaaatgggc ggcaaagaaa aaacaatggt
    19501 tttatcgtcc agacgaacat aaaagttact ggaatcgtga agaacacacg atagaagaaa
    19561 tccgcgcaaa atacggtaca accggacagc gcagggcgac agggtggcaa cgcgtggaaa
    19621 ccagagcgta accagaacgg gggcgagaag cccccgatat tatagccagc ccgtcttttg
    19681 gggcatcgaa cccaaaaaga cgggaacagc agccacgtag caaaaaggga aacagatgaa
    19741 ctcttttttt gaacaatatc accctgtctt tgaagttgtc tgccgtatcc tggggaacgg
    19801 ctggcgcgtg aacaaacttg atgattgttc gtctcgtata aaactgacgt caccgcagtt
    19861 taaaaattac tctgtgcata ttcggatgga aaaagaccgc ttttctgtgg tggggagtgt
    19921 ggatagtcgt tcctggcgta gtccgcatca tgtttgcacg ttatccagaa aacggaatcc
    19981 cgttgatatt gcagccgata tcgagcgaaa aattctggta aatgcttcgc aggaggtgtt
    20041 acaggcgatt gagtatgaga aacaccaggt ggaaaaaaag gacgaaattc tgatcctgaa
    20101 aggtatgtta tcgcaacttg tccagcttga aagctggtat ggggcattga caggctttaa
    20161 agctgaaaac ggattgaacg gtaaagtcac cgaacagggc gacagttatg atttacagat
    20221 tcggggcttg agtatagatc aacttgttaa aattacaggg tatttgaaac agttatgaga
    20281 aaaaataaag gtgatgtgac ttattttctt gaaaaagaag gtgacaatta caggcttaca
    20341 aaaaggataa aagccagaac aaatgttaaa atcgggaaca aaactacgaa aattacattg
    20401 tatgatgctg tactgaatga gaatgaatta caacatattg attttacgtg tgccggatta
    20461 agggaagacg atgagacgcc agttaagaat ttaattaagg agtttatgtt aaatgaaacc
    20521 cgataataca cctgaaaatg taaaaaaatt acgtttaaaa gcaggattaa cacaaaagga
    20581 atgcagtcat atttatggtg ttgggctaag aacctggcag aaaaaagagg aagttaatac
    20641 tcaaaacagt caaagtctgt cactggttga gtttgagttt cttttgttac ttgcaggaga
    20701 acatcctgaa tacgtgctct gcaaaaggga gtcaaaataa aatgacctgt gatggtttta
    20761 tattagtacc tggagttgcg ctcgtagctc gctgacgctc acacgcgcag tacagcgccc
    20821 cgccatgctg caatgcacca gggcggggta aactcgcttg cgctcgcgct aactgcggca
    20881 ttctgcggcg caaatcagca taatcatggc ctgctggtca gttgggcttt gcagggtgaa
    20941 gggatggcct gtgcaccctg gtacgttcgt cagcggctgc gccgcgcttc ggggcgatgt
    21001 ggctcctgtg ctgccctggt attttcgttc gccccttcca tccgcttacg cggattaaaa
    21061 aacgtgataa caatcactta tcgagtaaaa aaacgggaga aaacttgcaa atacgactta
    21121 aaggtcgtat tattgtttca tcgaaagggg aatggcccct ctcacaatag cccagcaggg
    21181 caggagattt aaaatgaaat gctcatctgt ttttacttca accactaatc atgttttcac
    21241 attcgaacga gtaacgcttt gcactattat ccttatgcat aaggatactg gacaacagta
    21301 tgttgtcata tttactgata acaataaaat tcgtgactat aaagccggaa ttgttcctca
    21361 gtttggcgaa cttaagcaga gtgatgttga tttagtcctt ttctacaggg acgaatatga
    21421 aaaatatttt gattcattaa aagatggcga tgaatgcttg agttttaaag attttattga
    21481 gtgtctttgt taagcattat gaatgcaggg gcatccctgc attttctgga aatttcaata
    21541 ttgattctga aattgtctat taacatcaat gcaagggaaa ttcatgaaaa cgttaaaaca
    21601 agccgcaatg cagtttgcat ctgatctgcg taataatcgc tgttttaaag ctgcttataa
    21661 tgatgctctg ttagagcttg atcgggaaga tttaaacgcc attgtacaat gcacatttct
    21721 ttttgagcca aaacgaacat tagccgaact tgataaatta attaaaaaga tagaagtaga
    21781 agaagattat gagttttgtg tatcccttga taaaggtaaa ggattacacg aagtcagaac
    21841 atttaaaagt gagcgtgagg cgttggatgc ttatcgcatt ttgactcttt acggatacac
    21901 agtaactata gaaaagaagg agggtaatga ctgactattc aaggtgtgat caggttgttc
    21961 caccaacctg atcacgttat cgacaatcaa aactgaatga ggtttttaaa tgtcaacgcg
    22021 gcagatcgta cagcttgctg attcccttgt caaaggctgg gaaaccagaa ttccggcaat
    22081 gaaatttagc gaatgggatc aattccggtg gtggttaaac tacttgcaag ggtatgaaat
    22141 gttttaaata aaaaaagggg ctttctgccc ctttgtattt tttgacaaaa atgccatgtg
    22201 taactataat tagttgcata tctatagggg atggatttat ggggaaaaca gataagctac
    22261 tggcaaagtt tttaaacagt aaaaaaacgt ttgaatggga tgaactggtc gttttgtttt
    22321 cctctttggg atatgtcaaa aaggaaatgc aggggtcaag agtgcggttt ttcaatgctg
    22381 aaatcaatca caccatatta atgcatcgtc cacatccgga aagttatatc aaaggtggta
    22441 cgctgaaagc aattaaacag aatctgaaag aggctggatt attatgaaac atttaaaata
    22501 taaaggatat ttaggcacgg ttgaacccga ttttgaaaat aatgtcctgt atggaaaact
    22561 ggcgtttatt cgggacctgg taacttatga ggcttctaca ttagctgaac tggaacagga
    22621 gtttaaaaca tctgttgatc tgtatttaca gtcttgtgtg gaggatggaa aggaacctga
    22681 taccccgttt aaaggcgtgt ttaacgtcag gcttgatcca gaactgcatc gtcgggtagc
    22741 agaaatggcg atggaagaag atttatcgtt gaatgccttt gttaataagg ctctggaaaa
    22801 agaagtcagc aatcatcgcg caggggctta attgcccttg tttttatgcc ctgtaagggc
    22861 atgggaggcg ctttgcgccg gaagccccgc agcgcagcga ggacttaaaa agcgttacgg
    22921 cttcagccgt aacaattccc agccctgcca ggcctgtttt ttctttcaca aatctttcag
    22981 ctatacgttt gcctgatggt cgaagacgaa agaatctttc agcaacgaaa tctggacata
    23041 tgcagcgacg attttatagc caggcacagt gccacatatg gtacaatcaa tgcaggatag
    23101 acaatccgat gtcaaatgtt aacatttgaa tcagattgtc caaaaacgat gtttttgcct
    23161 gtgcctgccg caggctctgt tggggtgact tatgaagatg atagcgttca ggctaagtga
    23221 tgatgagtta aaatttgcag aacataacgc tattttaagt ggatttacca gtatcaatgc
    23281 ttttgctaag cataacgtgc ttaatattga aaccaagcct gttaacatcc cagttaataa
    23341 tgaaccagca aagctggtat cagtgcgtct ttaccctcat gaaatagaac ttgttaaacg
    23401 taatgctgct cttcacggca tgagcatgag cagagaaatt gctattcgtg tgcgtcagtc
    23461 tttgttaaag agtgaagtct gtctttaccc tgatgaagtt aaggaactta aaaaactatc
    23521 aactgctgta gatcgtgttg gtcgcaacat tcattttatc ataaaaggtg aaagattctg
    23581 tacggttaac gatccagatt tcagaaaaga cgttattgaa gtgattgaac tgtgtaagca
    23641 aattgattca aaacttgaaa cgttaaccaa gagtgttgtt aacagatttg ggtgatttat
    23701 gggtgtgtat gtagaacagg aataccgtat taaacgcgct aaagggactg ggcgtgatcc
    23761 gaaatctcct aagttatcgg gaagacatat acatgccagt aaatcggctt ttaatcataa
    23821 agtcagacat ggtgcggaca agaaagcata tacctggaca cctacaaaag aagtcacttt
    23881 taaaatcact ggctcaggta aaacagccgc aggcatcaaa aatggtattg attacattac
    23941 ccgtaatggt gaacttgagg cttactgtta tgatggaaaa ggttcagagc aaacagggaa
    24001 aggagaagac tttaataggg agtttacttc aactttatct aaaggaaatg attactccag
    24061 gacatatcgt ggagaaaaca tcgatcatgt gaaaaacatg gtcttttcac caccaccaga
    24121 agctggagta agcagggaag acgctctgaa agctacggta gaatttctta aagagactta
    24181 tccaaaccat gctttcgttg ccgtatacca tgatgataaa gaagatcatc ctcatgtaca
    24241 tgttaatatt aaacttcgtg atgaagaaac aggaagaaga ttaagactta ctaaatctga
    24301 aaccaggaag ttcagaaacg gttttcatag aaaactgaaa ggtatgggat atgatgttac
    24361 cgctacctgg aaaaaagatc ccgaacgtaa acgggagatt gagcgattac aggctgaaaa
    24421 tccgaaaaga ctccggaatg tttacaaggt ggtggatttt ggggagactt catatcagaa
    24481 taaggcaggg gaaaaacgaa cacctttcct cacttatgaa actttaaaag gtggaaaaca
    24541 ggttacgatt tggggtaaag acctgaaaaa tcatttcgag tctgaaaagc tccagccagg
    24601 aacgcttatc aaagtaaaaa aactggctcc tacgcttgta cgtagtccaa tgtttaatga
    24661 tgatggaact gttgcaggct acagggaaac gcatcgcaat aactggcaga ttgaaaatat
    24721 tgcccttgag cggaacagag aacgtcaggt tcatgagcgt gaaaccaggc agccggaaga
    24781 gagcgacgtt aaaaagcagc tatccagaaa acatgagcaa ggtcacaata tcgggtttgc
    24841 tcttgaacac gggtttacaa aggattcaga agaacataaa aaactaagaa tacagcaaga
    24901 gaggaactgg aaaggcctgg gattttaatt tgattttggc gcaggtgaac aaagcggcta
    24961 ctttgctcac ctgctgatca caacaaccta cgagggaggt tatcatgact gagcatgatg
    25021 ctatctgcat ttctggactt catcaaattt ttagtgatga agaacatttg agtgagcaac
    25081 aaaaagacat tattctgatg tacgcttatg gctataccct gaatgaaatt gctgatttca
    25141 aaggactaaa gccttcaact gtcagaaaat atctcgattc tgtaagagct gaactgggag
    25201 gtgtctcgtt ggcagggatc agaacactgg tactcattcg gactaatgcc cttctcgtgt
    25261 caagtctttc caggatcagt gagaggggga atttatgaaa aaatatctgc tttttgcttt
    25321 gccgttcttc gttgttggtt gtagtgaaga agtaaaaagt gtggattggt gggggcagca
    25381 tcttacggaa gctaaacaaa agcaggcaga gtgtgagaag tctggctctg attctcagaa
    25441 ctgtaagaat gttaaacaag cattgtttat tcagtctcag aaggatgctc cagtccctac
    25501 attcgattaa ctaatgtttc aatcatacta gagcgcccat atgggcgctt ttttttgcat
    25561 gatcacacga atcataatat gaccattttg tcaatgttat ttgatcgtat tgttgagcat
    25621 tgttattttg cgaatcatgt tcgcatctaa aacaaatcca aaacacactt atagtggatt
    25681 gattaaatga aggagaaatc agataagtca tcggaaaaag gggtttatgt cacgataaaa
    25741 ctcgatccca ggttgaatgc atttatcgaa gagaaaacga aaacatcttt gcttagtaag
    25801 aggaagcaaa ttatttactt attaatgcag agtatgcgta ttgatggata tatggagcgt
    25861 tagctatggc gtctttattc ccagccttgt tgctgtgtgc ggcgtcagtt catccggata
    25921 cgataaatga tattgcacgg gtcgagagtg gcttcaatcc ttatgctgtg gctgaaatta
    25981 ttcctgaaag ggaacgcggt agctatggca aaagtgttat cagtcatatg cccaaaagta
    26041 aggaggaagc tctaacagtt atcagagaaa ttgaaaacag aaaaagacgt tattctgtcg
    26101 gattgatgca gattaccagc agtaattttt ctttttattc cacgtccgca gaaaaactcc
    26161 ttgattcgtg tgaaaacttg tctgttttcg aaaagattat cgtggattgt tataaacgag
    26221 ggaggagcct ggagaatgcg cttagttgtt actatacagg aaattttagt aacggtaagc
    26281 gaaaagaaaa agaatttaat aatacaagtt atgttgagcg aattggttat acagggaacg
    26341 aaaaaaatat gttgtaccag gtacaagaag caacggggga gaacagcgaa aaaatcgttc
    26401 acacaatgcg tcagttatct ggcccgaaac cattctgaaa tctgcttttg ttgataactc
    26461 acatccaaca aaagttatta attaacagga cttataaaat gtcatttaag aaatcagtat
    26521 gtaatcgtat cagttcatct gtcactgctg taatgcagcg taaaaataag gtagttatgg
    26581 cagtcgccgg ggcaacagtt gcatcaccgg cctttgcgga tggattttca aaagcggaaa
    26641 cactgctgga aaaggttaaa acagggctta gtggcctttc actggtcacg gtgactattg
    26701 cctgtctgtg ggtcggctac aaggttctgt ttggtggaag cacgattcgt gaatgctcac
    26761 ctatcatcat cggggcaatt gttatcgcca gtgcggctga agttgcatca atgatggtta
    26821 actaataaag cggggacgat agtatgtcta cactttataa agcgatgaca cgccctgcta
    26881 tgtatgtggg tgttcctgtc gtccctctta ctgtggttgc aggggcgtta tttctggcgg
    26941 gggtttatat cagcaagctg atctggctgg ctattcctgt cgcggttttt gttctcagaa
    27001 tgatcactaa acaggacgac cacattttta acctttactt tcttaagctg aaaatgctcg
    27061 gaaactcagt gtgtaatcgt ttttttggcg cacgagcgtt cctctccgga caatatgaag
    27121 ctgttgaaat tgatgagttt gttaatgcca tgaaactgaa tgagcgaatc actactggta
    27181 aatatatacc ctattcaagc catgttgata aaaatattgt caaaacaaag aacggtgatt
    27241 atgtagcgac atggcaactg atggggatta actttgagtc aatctctgcg gagatgttag
    27301 aaactattga ctcacaggtt gccactcttg ttcgttcatt ttccggactg cccgtttcat
    27361 tttataacca ttcatgcagg gcgagttttt atgatgcctt taccacaaaa tcaggtaata
    27421 aatatgctga tataatatca gattgttact atggttcgat gaagaaaaat aaattcaagg
    27481 ggaacactct ttactttacg ctgatctaca gaccagatgg gcgtgtagaa aaactggaaa
    27541 aacgcaaaaa aagtattaaa gaaaagaaag atgatattaa cattcatgtg aagagaatga
    27601 atgaaatgat taatacattc tctggcgctc ttgataaatt tacctgtaaa cttcttggta
    27661 tgtatgagga gaacggaaag gtattttctt ctcaactgtc attctataat tatcttctga
    27721 cagggaagtt gcaaaaaatc agagtcactg attctccggt gtataacgtt cttggtggag
    27781 tggatgtatt ttttaatcat gataccggac agatatgccg cattgatgga aataaattct
    27841 ttcgttctat tgaaattaaa gatttctgta gtgagactgc atccggtgtt tttgatgtgc
    27901 tccagtattc tgatgctgac tatattatta cgcattcata tacatccatg agcaaatcag
    27961 aagcattgag taccattaaa cgtgcggaaa aacagttaaa gagtacagaa gatgatgcgg
    28021 ttactcaatt gcaggaactg gaaaaggcaa aaaatgatat tgtgtccggt gatatatcct
    28081 ttggttacta ccatttcacg ctaatggtaa tggcggacag tatcagggag cttgacgaat
    28141 ctgtaagtaa aattacggca gactttactg acctgggcat tattccggca ttgtcaacca
    28201 tgtcattgcc agcggcatat tttgcccaac ttccggcggt ttttcattta aggccgcgtt
    28261 tatctccggt atcgaatgta aattttgttg aactggcatc attccataat ttctaccagg
    28321 ggaaacgcga taaaaactgc tggacagaag cggttgctat tctcaaaaca ccaagcaaac
    28381 aggcgtatta tctgaattta cataactcag tactgtttaa agatgaaaca ggtgaaaaga
    28441 atcttgctaa tactaaagtt ataggaacgg cgggttcagg taagacaatg tttctgtcat
    28501 atcttgcctg ttcattacag aaatacaata accccgaaac ttttgctgat tctgcaaaaa
    28561 ataagaagtt aacgtgtgtc tttcttgata aggacagagg ggctgaatta tgtatccgaa
    28621 tgcttggtgg ggaatattac accgtaaaaa gcggtgagcc gacaggatgg aatccttttg
    28681 ctctggaggc aacaaaacgt aaccgtattt ttgtcaagca actgatggag attctgtgta
    28741 ccagaaatgg tgaaagatta tcgacaagag aaagattgct tattagcgaa agtgttgatg
    28801 ctgtgatgga ttttcctcct ggcgaaatgc gggaatatgg cattaccaga atgcttgagc
    28861 atcttatgca gagagatgat cgggatgagc aggagaacgg gattatcctt cgtttgtccc
    28921 aatgggctaa tggtcaggct catggctggg tttttgataa tgctaaagac acattcaata
    28981 ttcagcatgt caataatttt ggcattgatg gcaccgagtt tcttgatgat ccgatggtat
    29041 gtgcaccaat tactttctat ctgctgtatc gcattacgca gcttctggac ggtcggcgtc
    29101 ttgttatctt cctggatgag ttctggaaat ggctacagga tgaagcattc agcgactttg
    29161 tctataacaa gctgaaaaca attcgtaagc tgaacgggct ggttattccg gcaactcagt
    29221 ctccggatga aatactgaag aataagattt cacgggctgt ggttgaagta tgcagcacca
    29281 gtatttatct ggctaaccct gatgccgatt acaatgatta tgtggaaggg cttaagctga
    29341 caccagaaga atttaatatt gttaaaaatc ttgatccaat gtcgagacag ttcctgatta
    29401 aaaaaagcag tcttaaaaag ggggatggta aatcattttc agcacttgcg acactcgatt
    29461 tatcgggact tggtggatat ctgaaaatat tgtctgccag tgcagataat ctggaaattt
    29521 ttgaaagtat ttatcatgaa ggaatggagc ctgatgactg ggttcctgaa tatctcgaac
    29581 gggcaatctg acaggtgaat ttatgaaacg tgtaaaaaca ttgatgctga tatcattatt
    29641 gacggcttca ttttatagta gcgcaggaat tccggttgct attgatgcca gtccggaatg
    29701 ggctgttgaa gccgcaagat ggacagaacg actcaaacaa tggtccgaaa ctgcgcaaca
    29761 ttatcagagt cagattcagg catataaaga ccagttagcc acggcaacag gtataaggaa
    29821 tattgcggca ttcacaaatg aactaagtaa tctacaatca gaactgacaa atatttataa
    29881 gcaggggaac agttatattt ctgattttac cagtaatccc gaaggtgcat tgagcagcca
    29941 ggctaaaaat ctgttcagca agtatggagc cttcgatatg tgtaatacgg gctatgaacg
    30001 taatgataat ctctgtaaag cacggattgt gtcaacggct gccagtattg aacagggaaa
    30061 tgagatcaat aagcagttat caagtgcaat gtctcagatt cagagtttaa gtagccgaat
    30121 tgaggcttca aaggatatca aagaatccca ggatttggct aacgcattac aggcgcagtc
    30181 gttaaaaatg caggcgataa aaatgcagta tgatgtctgg aataataaaa ataaagcaga
    30241 tcatgagatg ttggtcacac aagaacaaga ggcctttatt aaacagcaga aagaagctga
    30301 accattaact tttgattaaa aggtggatat atggctcagg ggtttttcgt taaatataat
    30361 tctaccgtta tggattcggt agataaaatc agctccagtt atcaaacaca atttgctaat
    30421 gatattatgt cgttagcaac ggtatcggtt acgttatatg tactatggaa aggatatcaa
    30481 atacttgcca gtaaaactca gacgcccttg caggatttag tatgggatct atcaaaattt
    30541 gcaatcataa tcatgtttat aactaatgcg gatgggtatt taactgcggc tactgatgcg
    30601 ttgcaaggca tgaaagatgg tttttctgga ggggtgagtg tctggcaaac tttagataac
    30661 ttatggaaaa gtacgcaaaa tcttggtgcg gaaatatact ctttagataa atcaacatat
    30721 gttaaagatc aaggtgtagt tggtcagttt ttaatatgga ctggctcgct tattttaatg
    30781 gctgttagtg tggttgtttt tttaacagca gatgtaacga tgaaactcct gattattaca
    30841 gcaccgatat ttatcttttg tctcatgttt ggttttattc ggagcatgtt taataattgg
    30901 ttacaatcgt tattctccag tattctgaca gttctatttg ccagtttagt gattcggata
    30961 gcaatggatt ttcaagggat gatcctttcc catgctattc gtgctgcaca aacagggaat
    31021 gtaaatcttg tatctacagg tgctatgggc tttatggctg ggtttttggg ggctttattg
    31081 gttcttatag ctaaaggttt tgctgtccag ttagcaggtg caggtgttga aggtgctgtt
    31141 cagggcgcag ccatgatggg gctgggtgct gctggtatgg ctactggtaa gtctctgatg
    31201 cttggcggtc gtgctggttt aggttttggc ttagggatgg ccggaagaac gggcatgaat
    31261 tctttatcag ggaaagcagg gaatctgatg gggcgtggtg cgcgaacagc cgctgaatgg
    31321 gcgggtgaaa agggatatca ggcgctggct ccgacaggtg ctttggggat gaaggcgaga
    31381 agacttgcat ccctggaaaa agcacgagcg aggaacgccg catgaaaata atgaaacgtt
    31441 ttttttatgt gatgtccctg aactggatac tggtgttcgt tgataaacat atcacctccg
    31501 gatggagcag gaagagaaag ctaataaccc agcttgttat tttaatcacg ggaattacct
    31561 tatttaaggt cttaggtgga atcagtcaga gctggttgtg acatgcttca ttattttgtc
    31621 atgatacttt attttgtcct aatccttctt ttaggagtgt ttacctgctt cgcaggaata
    31681 agacttggtt tagggcttgc ggggaaatgg agaaagggta tggatcttcg ctttttgctt
    31741 cttattggtg ggattctttt tcttcatctt ggcctttatc tcttaaaggt gttcgtgaac
    31801 gcttaataag gtgaatttat gcgatgtgtg attcttttgt ttttcctgtt tcagatttcc
    31861 ggatgtacca caaggaataa ccttccttct gatgtttccg gcgaactgga acccattaac
    31921 cacagccagg taatcagtta tgagtgaaaa aatcgaaaag aagattgaga cagctaaatc
    31981 cttcgaaaga caactctatg cagaaaatga acggtctaag aaaaatgcat ggcgtgttgc
    32041 agtggcagca tcattactgt ccttatgtct tggggctgca atttgtgttc ttgtgccatt
    32101 aaaagaaaaa gagcttgcta ttgtggctgt tggcgaaaaa acaggccgta cagaactgat
    32161 tactcaggtt aaacaggaaa agatcctgca atctgaagca cttgggcggt attttgtaaa
    32221 cacgtatatt actttacgtg aaggttataa ttatccgtct cttcagtatg attatgagac
    32281 tgtacagcta tatagctcga atacagtaaa ggacgattat cttcgtctgt ataacagcga
    32341 tatggctcct gataaaattt atcataataa cggttcttac gttgcggttg aggtcatctc
    32401 caatattatt tctgacgcaa ccgcgccaga taaactggcc tctgtgcgct ttaaaaaaac
    32461 gacgagaaac ttcacgaccg gacaggtttc agtttcatac tggacggctc gtgtgacata
    32521 tcgttttgaa ccagaaaagt cggttaaaag ttccagtcgt gagcttaatc ctctcggttt
    32581 tactgtaacc agttaccaga cagatcggga agtcaggggg gaatgatgaa gaaattattt
    32641 attgctacat gtttattgct tcctgggctt gtgtattcag cagcgactcc gttaccttct
    32701 ggatttgatg caaggatgca gactgtgagt tataacggtg cgaatacgac cgttatccgg
    32761 tcaaagacag gatttctcac atcagttgtt tttgatgaag gtgaggctgt tatcagtgca
    32821 aaggctggtt ttcctgccgg atgggaaatc acaaccgatg ataatgttgt ttacatcaat
    32881 ccgcgtcccg ttgttcagga acaggaaggc gatgaaggcg aaaaactgaa aaaggttttc
    32941 cagccaacag aaaaggaatg ggatacgaat ctttttgtca gaacaacaaa gcggatttac
    33001 agtctggatc taatccttct gtcagaagaa aaacaagcgc aacctgctta tgttgtacaa
    33061 ttccgctatc catcagaaat agcgaaaaag aatgcagaag aagtaaggct ggcaaaagaa
    33121 aaacaggaaa aactcagaca gaagaaactg atttctgaaa gttttgaaaa agccgatgcg
    33181 cctaaaaact ggaattattt catgcgagtc aatgaaaaat atgacagtcg tcgtatcgcc
    33241 cctgattttg cttatgataa tggcatattt acctttttag gttttaactc agggaaagta
    33301 ttc
//
