LOCUS       NZ_CP069653             2553 bp    DNA     circular CON 18-JUN-2023
DEFINITION  Escherichia coli O7:H4 strain MIN14 plasmid pMUB-MIN14-6, complete
            sequence.
ACCESSION   NZ_CP069653
VERSION     NZ_CP069653.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN17831486
            Assembly: GCF_017570585.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli O7:H4
  ORGANISM  Escherichia coli O7:H4
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2553)
  AUTHORS   Majewski,P.
  TITLE     Plasmid mediated mcr-1.1 colistin-resistance in clinical
            extraintestinal Escherichia coli strains isolated in Poland
  JOURNAL   Front Microbiol (2021) In press
REFERENCE   2  (bases 1 to 2553)
  AUTHORS   Majewski,P.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2021) Department of Microbiological Diagnostics
            and Infectious Immunology, Medical University of Bialystok,
            Waszyngtona 15A, Bialystok 15-269, Poland
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: Unicycler hybrid assembly v. v0.4.8-beta
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100x
            Sequencing Technology :: Illumina MiSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 06/18/2023 02:17:14
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,979
            CDSs (total)                      :: 4,859
            Genes (coding)                    :: 4,636
            CDSs (with protein)               :: 4,636
            Genes (RNA)                       :: 120
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 90
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 223
            CDSs (without protein)            :: 223
            Pseudo Genes (ambiguous residues) :: 0 of 223
            Pseudo Genes (frameshifted)       :: 61 of 223
            Pseudo Genes (incomplete)         :: 160 of 223
            Pseudo Genes (internal stop)      :: 47 of 223
            Pseudo Genes (multiple problems)  :: 45 of 223
            Pseudo Genes (short protein)      :: 4 of 223
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP069653.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2553
                     /organism="Escherichia coli O7:H4"
                     /mol_type="genomic DNA"
                     /strain="MIN14"
                     /serotype="O7:H4"
                     /isolation_source="upper respiratory tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:2810404"
                     /plasmid="pMUB-MIN14-6"
                     /geo_loc_name="Poland: Bialystok"
                     /lat_lon="53.12523570 N 23.15905989 E"
                     /collection_date="2016-07-21"
                     /genotype="ST-93"
     gene            1452..1997
                     /locus_tag="JSU15_RS24495"
                     /old_locus_tag="JSU15_24495"
     CDS             1452..1997
                     /locus_tag="JSU15_RS24495"
                     /old_locus_tag="JSU15_24495"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="WP_058109260.1"
                     /translation="MRERYLNKISNFDTARIVLFIAIQIISCIFMFWGAIPALIVFFSY
                     LAMRKECNLKPIEKSKDYISVYMLLTAALVLFLFVYKAGMFRRGGDYFLLVFVFILLYV
                     YHLAFHKLWLRTIDENSSIIIENGIFNFNRKNASVGFSRKDVNNIDTLLKLNEARKEGI
                     ISEDEFNKLKKRLNILDD"
                     /name="SHOCT domain-containing protein"
     gene            complement(2255..2368)
                     /locus_tag="JSU15_RS24500"
                     /old_locus_tag="JSU15_24500"
     CDS             complement(2255..2368)
                     /locus_tag="JSU15_RS24500"
                     /old_locus_tag="JSU15_24500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007772075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_059570819.1"
                     /translation="MHEPPVQSDRCALSGNYRLESNPERHAKAPLAAATGN"
                     /name="hypothetical protein"
     Typing          178..364
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="91.489"
                     /coverage="100.0"
                     /evalue="5.699999999999999e-63"
     Typing          complement(2..211)
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="92.417"
                     /coverage="96.0"
                     /evalue="4.0900000000000006e-79"
     Typing          complement(2256..2553)
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="96.99"
                     /coverage="87.0"
                     /evalue="7.99e-140"
ORIGIN
        1 tttgagcgtc agatttcgtg atgcttgtca ggggggcgga gcctatggaa aaacggcttt
       61 gcctcggccc tgtcgcttcc ctgttaagtc tcttcctggc atcttccagg aaatctccgc
      121 cccgttcgta agccatttcc gctcgccgca gtcgaacgac cgagcgtagc gagtcagtga
      181 gcgaggaagc ggaatatatc ctgtatcaca tattctgctg acgcaccggt gcagcctttt
      241 ttctcctgcc acatgaagca cttcactgac atcctcatca gtgccagcat agtaagccag
      301 catagtaagc cagtatacac tccgctagcg ctacgtgact ggttcagggc tgcgccccga
      361 aacccgctaa caacctctct gctactggat atcaccccat gccatctgaa tatgatgaac
      421 aacggggttc gactgatacg cagcttcccg gcgaatatga cctttttgat ttaatggcga
      481 aggttactga agagatcctg catgcccctg tggattctgg gccgcctgtc ggccgggaag
      541 cgttatagct ttttcgcttg cccgcgtacg tggtaatacg ccggtattac tttttatttc
      601 tttattttca tgtaattaca gtgggttttt aatacctggg tattactcgt gaaaacgaaa
      661 cattcctttt ttttctttta attcatgcag ttgcgatgaa aatcgcggct gcgttaggtg
      721 tatgaccatt ttgaggttga aaaataatca accatggagg gtgatttacg caggttaagg
      781 ggagatcgtg acaaaaaaag cgttcctcac tgtatcgttc atgcctgcat gaacgggtta
      841 ttcttcctga ctacagaatt acatcgctcc ttcagttcat acatcacttt tacaacactt
      901 ttacaacact ccttcggcct gtacatcacc tttacaacac ttttgcatca cttttacatc
      961 atttatacat cacttttaca tcattccttc agctcatacg tcacttttac gtcagttcca
     1021 tcctctgtgc catttgtaca tccgcccccc ttcctgaacg tgctgaaaac ggtttttccg
     1081 gctgtgccgg caacgcctca cggccacgca ataaattgcg tccccgttca ccgttttcgg
     1141 gtaagaagaa catcggaaac aggacttacc ccggctgaat ggcgtgaatt tccgcactat
     1201 gcacttgcgc gcatactcat gcatgccgta aaaacagaga ctgcgcgttt ctggcgggtt
     1261 ttcggggggg ttgttgcctg ttttaccggt ttcccgtcag aaacgccctc agagcctctc
     1321 aggcggtgca cgcaaggggg tgttatggta aatgccccat attcagttac tgcttttcta
     1381 ttttttgaaa taatattaat atcaggcgat tgattttaaa ttaattaact atgattgaat
     1441 ggtggtgatt tatgcgcgaa cggtatttaa ataaaatatc taattttgat acggcaagaa
     1501 tagtgttgtt tatagccatt caaattatct cttgtatttt tatgttctgg ggggctattc
     1561 ctgccttgat tgtttttttc tcatatttag ccatgaggaa ggaatgtaat ttaaagccga
     1621 tagagaagtc taaggattac atatccgttt acatgcttct tacagccgct ttggtactgt
     1681 ttcttttcgt gtataaagct ggcatgttta gaaggggtgg tgattatttc cttctcgttt
     1741 ttgtttttat tttgctttat gtttaccatc tcgcttttca taaattatgg ttgcgaacga
     1801 tagatgaaaa ctcatcaata ataattgaaa acgggatttt taattttaat cgtaaaaatg
     1861 catctgtagg atttagtcgg aaagatgtaa ataacattga tactcttctt aaattaaatg
     1921 aggctagaaa agaaggtatt ataagtgaag atgagtttaa taaattaaaa aaaagactaa
     1981 atattcttga cgactgattt ttttgctaac taaaggtaaa gcaagcttat gaatctttga
     2041 taagattgaa tatcttcttg tcattgattt tttatcgtcg taatcttctt ccctgcaaac
     2101 gaaaaaacca cccggcaagg tggtttttcg aaggttcgca agagttgacg ctcttttgaa
     2161 ccgcggtaac tggcttggag gagcacagtc accaaaactt gtcctttcag tttagcctta
     2221 accggcgcat gacttcaaga ctaactcctc taaatcagtt accagtggct gctgccagtg
     2281 gtgcttttgc atgtctttcc gggttggact caagacgata gttaccggat aaggcgcagc
     2341 ggtcggactg aacggggggt tcgtgcatac agtccagctt ggagcgaact gccttcccgg
     2401 aactgagtgt caggcgtgga atgagataaa cgcggccata acagcggaat gacaccggta
     2461 aaccgaaagg caggaacagg agagcgcacg agggagccgc cagggggaaa cgcctggtat
     2521 ctttatagtc ctgtcgggtt tcgccaccac tga
//
