LOCUS       NZ_CP069652             3688 bp    DNA     circular CON 18-JUN-2023
DEFINITION  Escherichia coli O7:H4 strain MIN14 plasmid pMUB-MIN14-5, complete
            sequence.
ACCESSION   NZ_CP069652
VERSION     NZ_CP069652.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN17831486
            Assembly: GCF_017570585.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli O7:H4
  ORGANISM  Escherichia coli O7:H4
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3688)
  AUTHORS   Majewski,P.
  TITLE     Plasmid mediated mcr-1.1 colistin-resistance in clinical
            extraintestinal Escherichia coli strains isolated in Poland
  JOURNAL   Front Microbiol (2021) In press
REFERENCE   2  (bases 1 to 3688)
  AUTHORS   Majewski,P.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2021) Department of Microbiological Diagnostics
            and Infectious Immunology, Medical University of Bialystok,
            Waszyngtona 15A, Bialystok 15-269, Poland
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: Unicycler hybrid assembly v. v0.4.8-beta
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100x
            Sequencing Technology :: Illumina MiSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 06/18/2023 02:17:14
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,979
            CDSs (total)                      :: 4,859
            Genes (coding)                    :: 4,636
            CDSs (with protein)               :: 4,636
            Genes (RNA)                       :: 120
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 90
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 223
            CDSs (without protein)            :: 223
            Pseudo Genes (ambiguous residues) :: 0 of 223
            Pseudo Genes (frameshifted)       :: 61 of 223
            Pseudo Genes (incomplete)         :: 160 of 223
            Pseudo Genes (internal stop)      :: 47 of 223
            Pseudo Genes (multiple problems)  :: 45 of 223
            Pseudo Genes (short protein)      :: 4 of 223
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP069652.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3688
                     /organism="Escherichia coli O7:H4"
                     /mol_type="genomic DNA"
                     /strain="MIN14"
                     /serotype="O7:H4"
                     /isolation_source="upper respiratory tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:2810404"
                     /plasmid="pMUB-MIN14-5"
                     /geo_loc_name="Poland: Bialystok"
                     /lat_lon="53.12523570 N 23.15905989 E"
                     /collection_date="2016-07-21"
                     /genotype="ST-93"
     gene            complement(join(3470..3688,1..300))
                     /locus_tag="JSU15_RS24460"
                     /old_locus_tag="JSU15_24460"
     CDS             complement(join(3470..3688,1..300))
                     /locus_tag="JSU15_RS24460"
                     /old_locus_tag="JSU15_24460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001447511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_001749510.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTEKMLNDEFRKLGESVSEAVTSNE
                     TKIRDAIALFTASTEKSLEKHREGVKEAMMQHRKDVLKLAGNTGMMLLGIVFLLFTASG
                     GTLWYLGGRIQTNLEEIRKQEETLQKLNAKTWGVEFVQDGNRKFLVLPYGKSAEVIPFQ
                     GKEWVHLKE"
                     /name="MbeB family mobilization protein"
     gene            complement(<338..706)
                     /locus_tag="JSU15_RS24465"
                     /old_locus_tag="JSU15_24465"
                     /pseudo=""
     CDS             complement(<338..706)
                     /locus_tag="JSU15_RS24465"
                     /old_locus_tag="JSU15_24465"
                     /inference="COORDINATES: protein motif:HMM:NF015397.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(673..987)
                     /locus_tag="JSU15_RS24470"
                     /old_locus_tag="JSU15_24470"
     CDS             complement(673..987)
                     /locus_tag="JSU15_RS24470"
                     /old_locus_tag="JSU15_24470"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_208232119.1"
                     /translation="MIVKFHAGGKGGGSGPVDYLLGRERNREGATVLQGNPEEVRELID
                     ATPFAKKYTSGVLSFAEKELPPGGREKVMASFERVLMPGLEKISTASCGWSTGTRDGLS
                     "
                     /name="hypothetical protein"
     gene            complement(977..1298)
                     /locus_tag="JSU15_RS24475"
                     /old_locus_tag="JSU15_24475"
                     /pseudo=""
     CDS             complement(977..1298)
                     /locus_tag="JSU15_RS24475"
                     /old_locus_tag="JSU15_24475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            1362..1553
                     /locus_tag="JSU15_RS24480"
                     /old_locus_tag="JSU15_24480"
     CDS             1362..1553
                     /locus_tag="JSU15_RS24480"
                     /old_locus_tag="JSU15_24480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165985.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            2398..2928
                     /locus_tag="JSU15_RS24485"
                     /old_locus_tag="JSU15_24485"
     CDS             2398..2928
                     /locus_tag="JSU15_RS24485"
                     /old_locus_tag="JSU15_24485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010287135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000012591.1"
                     /translation="MSGFIPFVGSKYENSIYGLKLLVLGLSHYGDADDNYPDFTKDVIA
                     ENAYTTGNRFFTILTNVLRLSRESITDDQRKITWENIAFYNYIQQIVGEKGRISPTKEM
                     WDSAREPFLSVIKQLQPNVILVLGTELWDHLPEVDGVEWCWIRHPSSSNFSYDQAFNNL
                     KKSIGMENIVYLP"
                     /name="hypothetical protein"
     gene            complement(3230..3463)
                     /locus_tag="JSU15_RS24490"
                     /old_locus_tag="JSU15_24490"
     CDS             complement(3230..3463)
                     /locus_tag="JSU15_RS24490"
                     /old_locus_tag="JSU15_24490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_004026237.1"
                     /translation="MTELEIHLLNALEQLQQDYMQRLSEWESAFVELQKMFSLTQRDNA
                     MLNERVMQLSQQVQHLSEQTERLSQLYSENWR"
                     /name="MbeD family mobilization/exclusion protein"
     Typing          complement(1639..1726)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="97.753"
                     /coverage="100.0"
                     /evalue="1.48e-35"
     Typing          complement(3..730)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="95.062"
                     /coverage="99.0"
                     /evalue="3.85e-178"
     Typing          complement(981..1207)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="96.053"
                     /coverage="99.0"
                     /evalue="5.909999999999999e-48"
     Typing          complement(3127..3686)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="94.33"
                     /coverage="99.0"
                     /evalue="7.780000000000001e-86"
ORIGIN
        1 aaacaggaga aagactatcc ccagtaacat catgcccgta ttccctgcca gctttaacac
       61 gtccttcctg tgctgcatca tcgcctcttt caccccttcc cggtgttttt ccagcgattt
      121 ctctgtcgag gctgtgaaca gggctatagc gtctctgatt ttcgtctcgt ttgaggtcac
      181 agcctcgctg acagactcgc cgagcttcct gaactcgtcg ttcagcattt tctctgtaga
      241 ttcggctctc tctttcagct ttttctcgaa ctccgcgccc gtctgtaaaa gattgctcat
      301 aaaacgctcc tttcagtctg atattcctcc cgccgttcgg gtctgcaatg ctgatactgc
      361 ttcgcgtcac cctgaccact tcaagccccg cctctgtgag cgcctgaatc acatcctgac
      421 gtccttttat ctctccggca tggtaaagtg cgtctatacc gcgtgtaacg ctctcagcaa
      481 gtgcctgttt cgtttccggc aggttatcag gaagggtcag cgtccggcgg ttctccgggg
      541 cgttggggtc atgcagtccg taatgttgat tcaccagcgt ctgccgggca tcaattctcg
      601 gcctgtctgc gcggtcgtag tacggctgga gacgttttcc ggtctgtagc tccatgttcg
      661 gaatgacaaa attcagctca agccgtccct tgtcccggtg ctccacccac aggatgctgt
      721 actgattttt tcgagaccgg gcatcagtac acgctcaaag ctcgccatca ctttttcacg
      781 tcctcccggc ggcagctcct tctccgcgaa cgacagaaca ccggacgtgt atttcttcgc
      841 aaatggcgtg gcatcgatga gttcccggac ttcttccgga ttaccctgaa gcaccgttgc
      901 gccttcgcgg ttacgctccc tccccagcag gtaatcaacc ggaccactgc caccaccttt
      961 tcccccggca tgaaatttaa ctatcatccc gcgccccctg ttccctgacg gccagacgca
     1021 gccggcgcag ctcatccccg atggccatca gtgcggccac tacctgaacc cggtcaccgg
     1081 aagaccactg cccgctgttc accttacggg ctgtctgatt caggttattt ccgatggcgg
     1141 ccagctgacg cagtaacggc ggtgccagtg tcggcagttt cccggaacgg gcaaccggct
     1201 cacccagcag acccgccgca tccataccgc cagttgttta ccctcacagc gttcaagtaa
     1261 cccgcatgtt catcatcagt aacccgtatc gtgagcatcc tctcgcgttt catcggtatc
     1321 attaccccat gaacagaaat cccccttaca cggaggcatc agtgactaaa caggaaaaaa
     1381 cagcccttaa catggcgcgc tttatcagaa gccagacatt aacgctgctg gaaaagctca
     1441 acgaactgga cgcggatgaa caggcagaca tctgtgaatc gcttcacgac cacgccgatg
     1501 agctttaccg cagctgcctc gcacgtttcg gggatgacgg tgaaaacctc tgacacatgc
     1561 agctcccgga gacggtcaca gcttgtctgt gagcggatgc cgggagcaga caagcccgcc
     1621 agggcgcgtc agcgggtttt agcgggtgtc ggggcgcagc catgacccag tcacgtagcg
     1681 atagcggagt gtatactggc ttacttatgc ggcatcagtg cggattgtat gaaaagtgca
     1741 tcatgtgagg tgtgaaatac cgcacagatg cgtaaggaga aaatgccgca tcaggcgcac
     1801 ttccgcttcc tcgctcactg actcgctacg ctcggtcgtt cggctgcggc gagcggtgaa
     1861 aacgatctga catgatcaaa atgatcttca tgcgctaaat gtacaaaagc cccgtgacag
     1921 aatcacaggg cttcggtact ctaattcttt gcagatacct gccaagatca gacgtagcaa
     1981 tcaaatctta gcagggctgg ctagccatgt cactaccagc ttaataccat tcaacgacat
     2041 taaaatcata tctgcacctt agacgctcag gtctacgccc cggactccgg caggataaac
     2101 aggcaaattg gtgggaacgt aacttaaccg cgaaagagtt aggctctacc ccgtctcgct
     2161 gtacgataac aagcacctga taaaatgagc gaataaggca gcagtaagcg ggaaagtgtg
     2221 attgcgagaa gtctgacact aaccccacac aaatcgaatc cgttgcaaga tgagacacac
     2281 aagaaaagag ctggacgaag gtcgctccgc tccccgtggt tttttatata cccaccacgg
     2341 gggcattagg tttttatgtt caaaaaacaa ccatcacccc gaagaaagga gtatgccatg
     2401 agtggtttca taccatttgt cggtagcaag tatgagaaca gtatctatgg gctaaaatta
     2461 ctggtcttag gtttgtcgca ttatggtgat gctgatgata actatccaga ttttactaag
     2521 gatgttattg cagagaatgc ctatacaaca ggtaaccgat tcttcacgat actgactaat
     2581 gtacttcgat tgtctaggga aagcataact gatgatcaaa ggaagataac ttgggaaaac
     2641 atagcgttct acaactacat acaacaaatt gttggagaaa aaggtcgaat atctcccact
     2701 aaagaaatgt gggatagtgc ccgagagcca ttcctaagtg taattaagca actccaacca
     2761 aatgtgatcc tcgttctcgg tactgaactt tgggatcatc ttcctgaagt tgatggtgtt
     2821 gaatggtgtt ggattagaca tccaagctct agtaatttct catatgatca agcatttaat
     2881 aacctcaaaa aatcaatagg catggaaaat atcgtttatt taccataacg tcacattaca
     2941 cgcaccgcta aaacgcgttc agcgcgatca cggcagcaga caggtaaaaa tggcaacaaa
     3001 ccacccgaaa aactgccgcg atcgcgcctg ataaatttta accgcatgaa tacctatgca
     3061 accagatggt acaggccaca ttaacccctc ttaatccact gaagctgcca tttttcatgg
     3121 tttcaccatc ccagcgaagg accatccagc gtgcgttcct gtatttccgg ctgacgctcc
     3181 cgttccaggg ataacgcatg ttcgcgctcc tgtatcagcc gtgcctctct tatctccagt
     3241 tctcgctgta taactggctc aagcgttctg tctgctcgct caagtgctgc acctgctgac
     3301 tcaactgcat gacccgctcg ttcagcatcg cgttgtcccg ttgcgtaagc gaaaacatct
     3361 tctgcaattc cacgaaggcg ctctcccatt cgctcagccg ctgcatatag tcctgttgca
     3421 gctgctctaa ggcgttcagc aaatgtattt ccagctctgt cactctgagt cactccttca
     3481 gatgtaccca ctctttcccc tgaaagggaa tcacctccgc tgatttcccg tacggaagga
     3541 caaggaattt cctgttcccg tcctgcacaa actccacgcc ccatgtcttc gcgttcagtt
     3601 tctgcaatgt ctcttcctgc ttcctgattt cttccaggtt cgtctgtatc cgtccgccaa
     3661 gataccagag cgtcccgcca ctcgcggt
//
