LOCUS       NZ_CP069651             4091 bp    DNA     circular CON 18-JUN-2023
DEFINITION  Escherichia coli O7:H4 strain MIN14 plasmid pMUB-MIN14-4, complete
            sequence.
ACCESSION   NZ_CP069651
VERSION     NZ_CP069651.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN17831486
            Assembly: GCF_017570585.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli O7:H4
  ORGANISM  Escherichia coli O7:H4
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4091)
  AUTHORS   Majewski,P.
  TITLE     Plasmid mediated mcr-1.1 colistin-resistance in clinical
            extraintestinal Escherichia coli strains isolated in Poland
  JOURNAL   Front Microbiol (2021) In press
REFERENCE   2  (bases 1 to 4091)
  AUTHORS   Majewski,P.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2021) Department of Microbiological Diagnostics
            and Infectious Immunology, Medical University of Bialystok,
            Waszyngtona 15A, Bialystok 15-269, Poland
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: Unicycler hybrid assembly v. v0.4.8-beta
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100x
            Sequencing Technology :: Illumina MiSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 06/18/2023 02:17:14
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,979
            CDSs (total)                      :: 4,859
            Genes (coding)                    :: 4,636
            CDSs (with protein)               :: 4,636
            Genes (RNA)                       :: 120
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 90
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 223
            CDSs (without protein)            :: 223
            Pseudo Genes (ambiguous residues) :: 0 of 223
            Pseudo Genes (frameshifted)       :: 61 of 223
            Pseudo Genes (incomplete)         :: 160 of 223
            Pseudo Genes (internal stop)      :: 47 of 223
            Pseudo Genes (multiple problems)  :: 45 of 223
            Pseudo Genes (short protein)      :: 4 of 223
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP069651.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4091
                     /organism="Escherichia coli O7:H4"
                     /mol_type="genomic DNA"
                     /strain="MIN14"
                     /serotype="O7:H4"
                     /isolation_source="upper respiratory tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:2810404"
                     /plasmid="pMUB-MIN14-4"
                     /geo_loc_name="Poland: Bialystok"
                     /lat_lon="53.12523570 N 23.15905989 E"
                     /collection_date="2016-07-21"
                     /genotype="ST-93"
     gene            136..426
                     /locus_tag="JSU15_RS24445"
                     /old_locus_tag="JSU15_24445"
     CDS             136..426
                     /locus_tag="JSU15_RS24445"
                     /old_locus_tag="JSU15_24445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001514113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_021533561.1"
                     /translation="MSRTLEQKIADAEARLQRLKAKSRSLDTAQKVIVGAALLAKVREP
                     GETQLRAWLLHLLKTEVTRQADVSRIQPLIDELNALPKPVPKEVSENDQQA"
                     /name="hypothetical protein"
     gene            959..1660
                     /locus_tag="JSU15_RS24450"
                     /old_locus_tag="JSU15_24450"
     CDS             959..1660
                     /locus_tag="JSU15_RS24450"
                     /old_locus_tag="JSU15_24450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001281399.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_208232117.1"
                     /translation="MSDLIAYKSNALVEASYKLTLQEQRFLLLCIGRLKSGSDAESTEL
                     QKTMTVTAEEYFDSFPDMGRKNAEVQLQEAIDRLWDRSIILKDDEKREEFRWIQYRAQY
                     AKGEGKAQITFSDAVMPYLTQLKGQFTRVVIKNISNLSRSYSIRIYELLQQFRSTGERI
                     ISLDDFRSMLDIEHKYQTYKSLNQQLLRPCVDELNQKSDLAVTLETIKKGRTVVALHFR
                     FKEDKQIKMTI"
                     /name="replication initiation protein"
     gene            complement(1909..4056)
                     /gene="mobQ"
                     /locus_tag="JSU15_RS24455"
                     /old_locus_tag="JSU15_24455"
     CDS             complement(1909..4056)
                     /gene="mobQ"
                     /locus_tag="JSU15_RS24455"
                     /old_locus_tag="JSU15_24455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016240513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobQ family relaxase"
                     /protein_id="WP_061089184.1"
                     /translation="MAIYHLSMKILSRSKGYSAVASAAYRAGEKILDERTGVIHDYTRK
                     NGVASAVILTPANAPAWCANRAELWNAVEKAERRKNSQLAREFELAIPRELAQDAARET
                     VLNFVRENFVSRGMIADVAFHNLGGSNPHAHIMLSMRAITPDGFGEKVREWNDWTHAET
                     WRGSWADHANRALANAGYQEEIDHRSYERQGVEKTPGIHLGKSACAMEKWGIETERGEQ
                     NRLINRLNLEIQVSRDELRNSELTEPARRMADLLNIELPDNATSYILHDIIEALPTDSN
                     AAWKLTSKAMSMMADMQATRRRWEDLNTERKEAMSHAASLKKSSPFSSGFSRIPLMQWV
                     APEYGREQEKIQSLSQRMEKLRQHHWKVEKQDIPASQKAFETQWQQWGASGLADLREQL
                     RKREEELRRKEQEETERRRAEQLRRNDNAVMDSGVLKAVVTGYGEAPMPGNGEMTCYLL
                     LHNRNGEYTLWGNELEKYRSRIFESVDLMRDRSGYICDRSEIGQRPPLQRVYSSATFEQ
                     LLTQVCQTWPQHTRNLRQPKTWPESFCLGEDRQPAMPSLAARKVDFTQGRLPPTLMPVM
                     SSVDRETRQLQLLLVMGVDDSMGGVVRLNGTLYPAFAVPTADNSQLVINALTDKGLRFA
                     GYGEAVNHDADSPARPAPELMQFHLKTWSEPLFAIVNTPEKQPDHLFRSLGFERTWDEW
                     QRDEYARTHATERRHDRGWSQ"
                     /name="mobQ"
     Typing          3702..4056
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          960..1659
                     /name="rep_cluster_1778"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="89.158"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          complement(1913..4089)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.311"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 ggcgcacaca aactcgacga aggagagttt gtataagtgc gcacttcgtg atttgctgtg
       61 cattatgcca gttcagtttt taaactacca gtgacgcgct aaaatgcgat ttttcaccgc
      121 gcacggagtt acggaatgag tagaacactc gaacagaaga ttgccgatgc tgaggccaga
      181 ttgcagcgtc tgaaggcgaa gagcaggagc ctggacacag cgcagaaggt cattgtggga
      241 gcggcattgc tggcaaaggt cagggagcct ggggaaacac aactacgagc ctggttgctt
      301 catctcctga agacagaggt aacacggcag gctgatgtga gcaggataca gccgttgatt
      361 gacgaactga acgcgttacc aaaaccagtc cccaaagaag tgtcagaaaa cgaccaacag
      421 gcgtgaatgc accgtccgct cgccgcagcc ccagcgaccg agcgttagcg agcgagtggg
      481 cgaggaagcg gaagtgaatc cgtcgccaga ttgagcacat acgcacggtt tttcgacagg
      541 tgatatgcgc tgaacccgca tgaatactgg atttccacag ggttgagcgg ggttttgcta
      601 aagcttgcga acctgctttt ttgtaacctg ttgttttcca tgattttttg ttttgaaccc
      661 cccaagtttt ccacaggctc gccgttaagt ttttagatct tgtttttaag atcttgtttt
      721 taatacttgt tttagggtcg aattttctta tgaaattcaa gagtttagaa gactataggt
      781 aatataattt aaggtaaagg caatatgatt taaggtggat aggcaatatg atttaaggta
      841 aaggcaatat gatttaaggt aatattcaca ggaaaaggga tattaaaatt aaatcatatt
      901 gcctttacct aaaggaatgt ttatatttta ctcattacct taggttgagg gatttacaat
      961 gtcggatctg attgcctaca aatccaatgc actggttgag gccagctata aattgactct
     1021 tcaggaacag cgattcttgt tactttgcat tggtcgattg aagtcgggtt ctgatgcaga
     1081 atccactgaa ttgcaaaaga ccatgactgt taccgccgaa gagtatttcg actcattccc
     1141 tgatatgggg cggaaaaatg ccgaggttca gctacaggaa gcaattgatc gtttgtggga
     1201 caggtcgatc atccttaagg acgatgagaa gcgcgaggag ttccgctgga tccagtatcg
     1261 ggcacagtac gctaaaggtg agggcaaagc ccaaatcact ttttcagatg cggtcatgcc
     1321 ttatctgaca cagttgaagg ggcaatttac tagagttgta attaagaaca tctccaattt
     1381 atcaagaagt tacagcataa gaatatacga acttctccag caattccgca gcacagggga
     1441 acgtattatc tccctcgatg attttcgctc aatgcttgat atagagcata aatatcaaac
     1501 atataagtct ctgaaccagc aattattaag accttgcgtt gacgaactga atcaaaaaag
     1561 cgacttggct gtaacgcttg agacgatcaa gaaggggcgc acggttgtag ccctgcattt
     1621 tcgcttcaag gaagacaaac agatcaagat gacgatctag aatcgatttt aaggcctcta
     1681 aaacgctctc tgacgcgttt tttttgtgtt tggcatgcga aggtactggt gaagtaaaaa
     1741 attgcgtcat aaacgctctg gcacagtgaa aaaagaaccc ccaatttttc gggggcggga
     1801 aaactataaa gttgatttgt gttatgtgtg tgcttcttgc attcatccct gtcgatgaac
     1861 atcgacgaac ataacacgat gttaaacaaa acaaaatatt ggtcacattc actgtgacca
     1921 accgcgatca tgtctcctct cggtggcgtg tgttcgggca tattcatcac gttgccattc
     1981 atcccaggtg cgctcaaagc ccagcgagcg gaacagatga tccggctgtt tttctggcgt
     2041 gttgacgata gcaaaaagcg gttcgctcca ggttttcaga tgaaactgca taagttccgg
     2101 cgcaggacgt gccggactgt cagcatcgtg gtttaccgct tcaccatagc ctgcaaagcg
     2161 taaacctttg tcggtcagtg cattgattac cagctggctg ttgtcggctg tggggacggc
     2221 aaacgcggga tacagcgtac cattaagccg gacaacaccg cccatagagt catccacacc
     2281 catgaccagc aaaagctgaa gctggcgggt ttcacggtcg acagaactca ttaccggcat
     2341 cagagtcggt ggcagacgtc cctgcgtaaa atccaccttc cttgccgcca gtgatggcat
     2401 ggctggctgg cggtcttctc caagacagaa agactccggc catgttttcg gctggcggag
     2461 attacgggta tgttgcggcc atgtctggca aacctgcgtc agcagttgct caaaggtcgc
     2521 agaggaataa acacgttgca gtggtggacg ttgaccgatt tcgcttcggt cacagatata
     2581 accactcctg tcacgcatta aatcaacact ctcaaaaatt ctggagcggt atttctccag
     2641 ctcgttcccc cagagcgtat attctccgtt ccggttgtga agcagaaggt aacaggtcat
     2701 ttccccgtta cctggcatgg gggcctcgcc ataccccgta acgactgctt tcagtacccc
     2761 tgaatccata acggcattgt catttctcct gagctgctcc gcccgacgac gttctgtttc
     2821 ctcctgctcc ttccggcgca attcctcttc acgtttacgc aactgttctc ggagatctgc
     2881 aaggccagat gcaccccact gctgccactg tgtttcaaat gctttttgac tcgcgggaat
     2941 gtcctgtttc tcaaccttcc agtgatgctg ccgcaatttt tccatgcgct ggctcaacga
     3001 ctggattttt tcctgttcgc ggccgtattc cggtgcaacc cattgcatca ggggaatacg
     3061 ggaaaaaccg gacgaaaacg gcgatgattt tttcaggctg gcagcatggc tcatcgcttc
     3121 ctttcgttcg gtgttcagat cttcccagcg tctgcgcgtc gcctgcatat ctgccatcat
     3181 cgacatggct ttactggtga gtttccatgc tgcgttactg tccgtcggca acgcttcgat
     3241 gatgtcatgc agtatgtacg aagttgcgtt gtcaggaagc tcaatattca gaaggtcagc
     3301 catacggcgt gcgggctctg tcagctcact gttcctgagt tcgtcgcggg aaacctgtat
     3361 ttccaggtta agcctgttaa tcaggcgatt ctgttcgccg cgttctgttt cgatgcccca
     3421 cttttccatc gcacaggccg actttccgag gtgaatgcca ggcgttttct caaccccctg
     3481 tcgctcgtat gaacgatggt ctatctcttc ctgataaccg gcgttcgcca gcgccctgtt
     3541 cgcatggtct gcccatgatc cccgccatgt ttcggcgtga gtccagtcat tccactcacg
     3601 gactttctcc ccgaagccgt caggcgtgat tgccctcatg gatagcatga tgtgcgcgtg
     3661 tgggtttgac ccgcccagat tgtgaaaggc cacgtcggcg atcatgccgc gactgacaaa
     3721 gttttccctt acgaagttga gaactgtttc ccgtgcggca tcctgcgcca gttcacgggg
     3781 gatggcgagt tcgaactccc gcgcaagttg tgagtttttc cgccgctcgg ctttttcaac
     3841 agcattccag agttcggcgc ggttcgcaca ccatgctggg gcgtttgctg gcgtcagaat
     3901 cactgcgctg gctacaccgt ttttgcgggt gtagtcgtga atcactccgg tgcgctcatc
     3961 cagtattttt tctccggcac gataggcagc agaagcaacg gcgctgtagc ccttgcttct
     4021 ggaaagaatc ttcatgctga gatgataaat tgccatcgtg tcgttgttgc cgttttcttt
     4081 tagccccgca g
//
