LOCUS       NZ_CP069585             5251 bp    DNA     circular CON 17-JUN-2023
DEFINITION  Escherichia coli strain FDAARGOS_1265 plasmid unnamed2, complete
            sequence.
ACCESSION   NZ_CP069585
VERSION     NZ_CP069585.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN16357407
            Assembly: GCF_016890045.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5251)
  AUTHORS   Sokurenko,E., Tallon,L., Sadzewicz,L., Zhao,X., Boylan,J., Ott,S.,
            Bowen,H., Vavikolanu,K., Mehta,A., Aluvathingal,J., Nadendla,S.,
            Yan,Y. and Sichtig,H.
  TITLE     FDA dAtabase for Regulatory Grade micrObial Sequences (FDA-ARGOS):
            Supporting development and validation of Infectious Disease Dx tests
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5251)
  AUTHORS   Sokurenko,E., Tallon,L., Sadzewicz,L., Zhao,X., Boylan,J., Ott,S.,
            Bowen,H., Vavikolanu,K., Mehta,A., Aluvathingal,J., Nadendla,S.,
            Yan,Y. and Sichtig,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2021) Institute for Genome Sciences, University of
            Maryland School of Medicine, BioPark II, 801 W. Baltimore St., Suite
            619, Baltimore, MD 21201, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: SMRT v. 8.0.0, HGAP v. 4, SPAdes v. 3.14.1
            Genome Coverage                   :: 842.96x
            Sequencing Technology             :: Pacbio; Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 06/17/2023 04:40:36
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,408
            CDSs (total)                      :: 5,295
            Genes (coding)                    :: 5,024
            CDSs (with protein)               :: 5,024
            Genes (RNA)                       :: 113
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 100 of 271
            Pseudo Genes (incomplete)         :: 199 of 271
            Pseudo Genes (internal stop)      :: 44 of 271
            Pseudo Genes (multiple problems)  :: 65 of 271
            Pseudo Genes (short protein)      :: 3 of 271
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP069585.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5251
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="FDAARGOS_1265"
                     /host="Homo sapiens"
                     /culture_collection="FDA:FDAARGOS_1265"
                     /db_xref="taxon:562"
                     /plasmid="unnamed2"
                     /geo_loc_name="USA: WA"
                     /collected_by="ARMADA"
     gene            complement(join(4424..5251,1..513))
                     /locus_tag="I6J95_RS27160"
     CDS             complement(join(4424..5251,1..513))
                     /locus_tag="I6J95_RS27160"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="WP_251301696.1"
                     /translation="MLSFAEAELPPGQREQIMASFERVLMPGLDKDQYSILWVEHTDKG
                     RLELNFLIPNTELLTGKRLQPYYDRADRPRIDAWQTVVNGRLGLHDPNAPENRRLLVTP
                     SALPETKLEAAQAITRGLLALASSGELKTREDVTGALTAAGFEVVRTTKNSISIADPDG
                     GRNIRLKGAIYEQSFSAGEGLGAAIESAAAEYRRDAESRIQRARAVCKSGTERKREENQ
                     RRHQRPRAGYDRGDAVEPAERAAYGRPDVADHHPGLGTADRHEREHSVVAGAADHRELP
                     DYPGAGADAGHAVGEEPRRAAVALRGEETVLRQSEPESGSLRGGRELDGTGEEIAHDGT
                     GKTVADRIRAATSGLLAKAGRVGERLRGMAEDVWSYATGERGAERARHALESAGGELEQ
                     AVTAFEPVVQRHEMAVAAERAYEQKQHEKELAETRSRQRSYDGPTLG"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(671..991)
                     /locus_tag="I6J95_RS26710"
                     /old_locus_tag="I6J95_26720"
     CDS             complement(671..991)
                     /locus_tag="I6J95_RS26710"
                     /old_locus_tag="I6J95_26720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_014386475.1"
                     /translation="MLTMWVTEDEHRRLLERCDGKQLAAWMRQTCLDEKPARAGRLPSI
                     SPALLRQLAGMGNNLNQIARKVNAGGAGHDRVQIVAALMAIDAGLERLRHAVLEKGGDD
                     DR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            1628..1789
                     /locus_tag="I6J95_RS26715"
                     /old_locus_tag="I6J95_26725"
     CDS             1628..1789
                     /locus_tag="I6J95_RS26715"
                     /old_locus_tag="I6J95_26725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001318243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_023144844.1"
                     /translation="MHEPPVQSDRCAFSGNYHLESNPERHGSSPLAAAIGNGLEEVSLE
                     VLRQLRLN"
                     /name="hypothetical protein"
     gene            complement(1942..2562)
                     /locus_tag="I6J95_RS26720"
                     /old_locus_tag="I6J95_26730"
     CDS             complement(1942..2562)
                     /locus_tag="I6J95_RS26720"
                     /old_locus_tag="I6J95_26730"
                     /inference="COORDINATES: protein motif:HMM:NF025084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RloB family protein"
                     /protein_id="WP_023312615.1"
                     /translation="MSKKHGNNKKVPQVMYIYCEGEKTEPLYLEAYISDRSKRTLSVFK
                     IPKTRKNTPEQLVDEAIKKKNSSSSAEGDVFWVVYDQEHLTPSSISCHQKAWNKANRHG
                     INIAISCVCFELWLLLHFGFTTKDFSSYDNLMSSSPLKKLITNYDKGSKLTYLAIRERI
                     GTAKDNARKLDIYAKTNYKNTHEPYLFPCYTSFHELLKAIDDF"
                     /name="RloB family protein"
     gene            complement(2555..3910)
                     /locus_tag="I6J95_RS26725"
                     /old_locus_tag="I6J95_26735"
     CDS             complement(2555..3910)
                     /locus_tag="I6J95_RS26725"
                     /old_locus_tag="I6J95_26735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000498067.1"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA];
                     GO:0016887 - ATP hydrolysis activity [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="WP_014386473.1"
                     /translation="MFVYLTIENFRSVKEKSTLNLSASGSSNHLTTHIYKNDGMNVGTL
                     MSAGIYGANASGKSNVLMAFEAIKFIATESGDLKEGKRIPCYEPYALSSATNEAPVKFE
                     AEFYSVEGNRFSYEVAFIKNRILFESLDYYPSRVKANLFTRDESDTWETIKFGGHYKGG
                     IKKIPFFPNNSYLAKAGNNAASPDIIKEAYNFFRKGIRHIGLNEKIRISSFGEREDVVN
                     ETAKVLCMVDTGVSGISVREVEAEIPIKMDEEIPQEIKEIIEEDYKYRYMFSHPTDEGG
                     SVSFPMGRESEGTQKLFEIIPLIRSAFNDSMVVIVDELDNSLHPHIADLIVKLFNDPDV
                     NKKGSQLVFSTHNMQLMAPEKMRRDQIWFCEKNKGASSLYSLDDFDKKKIKTTTPYAAW
                     YDEGRFGGVPDINYLKVASFISGDISLVMPDIDVKELSDGFFEEFDGDLSDE"
                     /name="ATP-binding protein"
     Typing          complement(1106..1278)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="84.659"
                     /coverage="100.0"
                     /evalue="9.74e-41"
     Typing          1225..1738
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.617"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          complement(2..708)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="90.678"
                     /coverage="85.0"
                     /evalue="3.16e-135"
ORIGIN
        1 tcccttcagt cggatgtttc gccccccgtc cgggtcggca atgctgatac tgttttttgt
       61 ggtgcggacc acctcaaaac ctgccgctgt gagcgcccct gtgacgtcct cgcgcgtttt
      121 gagctccccg gatgaggcta aggccagcaa cccacgtgta atcgcctgag cggcttccag
      181 cttcgtttcc ggcagtgcgg acggcgtgac cagtaaccgt cggttctccg gggcgtttgg
      241 atcatgcagc cccagcctgc cgttcaccac ggtctgccag gcatcgatgc gcggccggtc
      301 ggcgcgatcg tagtacggct gcaggcgttt cccggtcagc agttcagtgt tggggatcag
      361 gaaattcagc tccagccgcc ccttgtcggt gtgctccacc cacagaatgc tgtactggtc
      421 tttatcgagc ccgggcatca gaacccgctc aaagctggcc atgatctgct cccgctgccc
      481 gggcggcagt tcagcctccg cgaacgacag cacgccggag gtgtactttt tcgcatacgg
      541 cgaggcatcg ataagctccc ggacttcctc cggttttccc tgcagcacgg tcgccccctc
      601 gcgctgccgg tcttttccca gcaggtaatc caccggaccg gcaccaccac cgcgcccccg
      661 ggggtgaaat ttaacgatca tcatcccccc ctttttccag caccgcatgc cgcagccgct
      721 ccagtcccgc gtcgattgcc atcagtgcgg ccacaatctg cacccggtca tgaccggcac
      781 caccggcatt cactttccgg gcgatctggt tcaggttgtt ccccataccg gcaagctggc
      841 gcagcagcgc cggcgatatc gacggcagtc tgccggcacg cgccggcttc tcatcgaggc
      901 aggtctgacg catccacgcc gccagctgct tgccgtcgca ccgctcgagc agccgccggt
      961 gttcgtcttc cgtcacccac atcgtgagca tcttgttgcg tttgtctgcc agagtagcct
     1021 cctgataaca ccgggacggg cgggaaatgt ttcccggtcg gcacggtgtt ggacaagccg
     1081 caggcgcgtc agtcgctttt agcgggtttc ggggcgcagc cctgaaccag tcacgtagcg
     1141 ctagcggagt gtatactggc ttattatgtt ggcatgagtg cggtaatcag gaaagtgctt
     1201 catgtgacag aggaaaatgc agctgcgggc gtaaggcaga aaatggaagt tcagaggata
     1261 tgacgcctcc tcgctcactc agtcgctgcc gctcctgccg ttcggctgcg gcgggcgtta
     1321 cctgcacacg gagaagagcg aaggcatatg gcatgacaaa cggggcagaa aaaggatgcg
     1381 ccgctgccgt ttttccatag gctccgcccc cctgacaagc atcacgaaat ctgacgctca
     1441 aatcagtggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggtggc
     1501 tccctcgtgc gctctcctgt tcctgccttt cggtttaccg gtgtcattcc gctgttatgg
     1561 ccgcgtttgt ctcattccac gcctgacact cagttccggg taggcagttc gctccaagct
     1621 ggactgtatg cacgaacccc ccgttcagtc cgaccgctgc gccttctccg gtaactatca
     1681 tcttgagtcc aacccggaaa gacacggttc atcgccactg gcagcagcca ttggtaacgg
     1741 attagaggaa gtgagtcttg aagttctgcg ccagttaagg ctaaactgaa aggacaattt
     1801 ttggtgactg cgctccctct aagccagtta cccggtacat aagttggtag ctcatgtacc
     1861 ttccaaaaac caccgttggt agcggtggtt tttatttttc atagattaca gcctgtaata
     1921 agccatggtc aatggattac ttcaaaagtc gtcaatcgcc ttaagtagct catggaatga
     1981 tgtataacat ggaaatagat aaggttcgtg cgtgttttta taattagttt tagcgtaaat
     2041 atctagcttt cttgcgttat ctttcgcagt accaatcctc tctcttatag caaggtaagt
     2101 tagtttacta cctttatcat agttagttat taattttttt agcggggagc tagacatcaa
     2161 attatcatac gacgaaaaat ctttggttgt gaagccgaaa tgcaacaata accataattc
     2221 aaaacaaaca caggatattg ctatatttat cccatgccta ttagctttat tccatgcctt
     2281 ttgatgacat gaaatagacg aaggcgtcag atgctcttga tcataaacaa cccaaaagac
     2341 gtcaccttct gcggaagatg atgaattctt tttctttatc gcctcatcca ccaactgctc
     2401 aggtgtattt tttcttgttt taggtatttt aaatacactt aaagttcttt tagatctgtc
     2461 tgaaatataa gcctcaaggt ataatggttc ggttttctca ccctcacagt atatatacat
     2521 tacttgtggg actttcttat tatttccatg ttttttactc atcagaaaga tccccgtcaa
     2581 actcttcaaa aaaaccatca gacaattctt tgacgtcaat atccggcatt accaaggata
     2641 tatcaccaga tataaatgag gctaccttta agtagtttat gtcgggaacc ccaccaaacc
     2701 taccctcatc ataccaagct gcgtaaggtg ttgttgtttt tatctttttc ttatcaaaat
     2761 catctaaact ataaagagac gacgcacctt tatttttttc acaaaaccat atctgatctc
     2821 ttctcatttt ctctggagcc attaactgca tattatgggt tgagaataca agttgtgagc
     2881 cttttttatt gacatcagga tcattaaata acttaacaat caagtcagca atatgcgggt
     2941 gcaagctatt atctaactca tcaacaatta caaccatact atcattaaac gcagatctaa
     3001 ttagaggtat gatttcaaat aatttttggg tgccttcaga ctctctcccc atagggaaag
     3061 aaacactccc cccttcatca gtaggatgag agaacatata acgatattta taatcttctt
     3121 caattatctc ctttatttct tgagggattt cctcatccat ttttattggt atttccgcct
     3181 caacctcccg tacagatata ccggaaacac cagtgtcaac catgcataaa accttggccg
     3241 tttcatttac gacgtcctcc ctctccccaa aagaagatat tcttatcttt tcatttaaac
     3301 ctatatgtct aattcccttt ctaaaaaagt tataagcttc tttaattatg tctggtgatg
     3361 cggcattatt acctgctttc gctaagtatg aattattagg gaagaatggt atttttttaa
     3421 tacctccttt ataatgccca ccaaacttga ttgtttccca ggtgtcgctc tcatctctag
     3481 taaataaatt tgctttaacc ctagatggat aatagtctaa tgattcaaat aaaatccgat
     3541 ttttaataaa agcaacttca taactgaacc gattcccctc aacagaataa aattcagcct
     3601 caaactttac aggagcctca ttcgtagccg aagaaagagc atatggctca tagcagggaa
     3661 tccgtttacc ttctttcaaa tcaccgcttt ctgttgcgat aaattttata gcttcaaaag
     3721 ccatgagaac gttagattta ccagacgcat tagcaccata aatcccagca ctcatgagcg
     3781 ttccaacatt cattccatca tttttataga tatgagttgt taaatggtta ctagaaccgg
     3841 atgcgctcaa gtttaatgtc gatttttcct taaccgacct aaaattttca atagtcaaat
     3901 aaacaaacat gcatacctcc taacggggat ttttcgccaa aaaagcgaaa aatcaatcat
     3961 aggaaagata tcctatatgt tttaattttg gaacaacatc ttccttaaaa aacgttgtgc
     4021 tatttataat tacacgctat ttaccataac atctgttgcc cgcaccgctc cggacaggtt
     4081 cgccacaaac ggggcggcag tcaggtaaaa ccggcaacaa accacccgat acaccgcccc
     4141 gttcgtgaca gataaatttt acccggctgc atggtcatgc agccagatgc atacggtctg
     4201 atttcaggcc agaatccgga tgaacgcctt aaaaacagga aaatgcttct ccagccagtc
     4261 ccagatctcc agcgccatgt acaggcaatt cagcaccgtc tcaatcacgt taaacagtct
     4321 cataatccct ccgttgtata cgctgtgatg gaaacatcac gaccgtattt agcgggaaat
     4381 ttatgagccc ttcttcttgg ggtttgtcac tggatgcgcg cagtcagccc agcgtcggac
     4441 cgtcatagct ccgctgccgg gaacgggttt ccgccagctc tttttcatgc tgtttctgct
     4501 cgtatgcccg ttcggcggcc accgccattt catgccgctg cactactggc tcaaacgctg
     4561 taactgcctg ctcaagttcg ccacctgctg actcaagcgc gtgacgcgct cgttcagcgc
     4621 cgcgttctcc cgttgcataa gaccagacat cttctgccat tccgcgaagg cgctctccca
     4681 ctcgtccagc ctttgcgagt agtcctgatg tagctgctct aatgcggtca gcaactgttt
     4741 ttccagttcc gtcatgggct atttcctctc cagtaccatc cagttccctt cctccccgta
     4801 agctcccgct ttcgggttca ctttgacgca ggacagtttc ttctccccgc agagcgacag
     4861 ctgcacgccg tggttcttct ccgacagcat ggcctgcgtc cgctcctgcg cccggatagt
     4921 ctggtaattc ccggtgatct gctgcccctg ccaccacaga atgctcccgc tcgtggcgat
     4981 cagcagtacc gagaccaggg tgatggtcag ccacgtccgg ccgaccatac gcagcacgct
     5041 cagccggttc gactgcatcg ccgcggtcat accctgctcg tgggcgctga tggcgtcgct
     5101 gattctcttc gcgcttgcgc tcagttccgc ttttacagac tgctcgtgct cgctgaatgc
     5161 ggctttcagc atctcgccgg tactctgctg ctgcgctttc gattgctgct ccaagtcctt
     5221 cgccagcgct aaaagactgc tcataaatgg c
//
