LOCUS       NZ_CP069580             3447 bp    DNA     linear   CON 17-JUN-2023
DEFINITION  Escherichia coli strain FDAARGOS_1250 plasmid unnamed10.
ACCESSION   NZ_CP069580
VERSION     NZ_CP069580.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN16357392
            Assembly: GCF_016890005.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3447)
  AUTHORS   Sokurenko,E., Tallon,L., Sadzewicz,L., Zhao,X., Boylan,J., Ott,S.,
            Bowen,H., Vavikolanu,K., Mehta,A., Aluvathingal,J., Nadendla,S.,
            Yan,Y. and Sichtig,H.
  TITLE     FDA dAtabase for Regulatory Grade micrObial Sequences (FDA-ARGOS):
            Supporting development and validation of Infectious Disease Dx tests
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3447)
  AUTHORS   Sokurenko,E., Tallon,L., Sadzewicz,L., Zhao,X., Boylan,J., Ott,S.,
            Bowen,H., Vavikolanu,K., Mehta,A., Aluvathingal,J., Nadendla,S.,
            Yan,Y. and Sichtig,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2021) Institute for Genome Sciences, University of
            Maryland School of Medicine, BioPark II, 801 W. Baltimore St., Suite
            619, Baltimore, MD 21201, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: SMRT v. 8.0.0, HGAP v. 4, SPAdes v. 3.14.1
            Genome Coverage                   :: 770.24x
            Sequencing Technology             :: Pacbio; Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 06/17/2023 05:03:03
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,029
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,669
            CDSs (with protein)               :: 4,669
            Genes (RNA)                       :: 119
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 241
            CDSs (without protein)            :: 241
            Pseudo Genes (ambiguous residues) :: 0 of 241
            Pseudo Genes (frameshifted)       :: 65 of 241
            Pseudo Genes (incomplete)         :: 178 of 241
            Pseudo Genes (internal stop)      :: 44 of 241
            Pseudo Genes (multiple problems)  :: 45 of 241
            Pseudo Genes (short protein)      :: 4 of 241
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP069580.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..3447
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="FDAARGOS_1250"
                     /host="Homo sapiens"
                     /culture_collection="FDA:FDAARGOS_1250"
                     /db_xref="taxon:562"
                     /plasmid="unnamed10"
                     /geo_loc_name="USA: WA"
                     /collected_by="ARMADA"
     gene            119..637
                     /locus_tag="I6J80_RS24805"
                     /old_locus_tag="I6J80_24820"
     CDS             119..637
                     /locus_tag="I6J80_RS24805"
                     /old_locus_tag="I6J80_24820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001447511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_204147844.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTEKMLNNEFRRLGESVSEAVTSNE
                     TKIRDAIALFTASTEESLEKHREGVKEAMRQHRRDVLKLAGNTGMMLLGIVFLLFTASG
                     GTLWYLGGRIQANLEDIRKQEETLQKLNAKTWGVEFVQDGNRKFLVLPYGKSAEVISFQ
                     GKEWVHLKE"
                     /name="MbeB family mobilization protein"
     gene            644..877
                     /locus_tag="I6J80_RS24810"
                     /old_locus_tag="I6J80_24825"
     CDS             644..877
                     /locus_tag="I6J80_RS24810"
                     /old_locus_tag="I6J80_24825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137445.1"
                     /translation="MTELETHLLNALEQLQQDYMQRLSEWESAFVELQKMFSLTQRDNA
                     MLNERVMQLSQQVQHLSEQTERLSQLYSENWR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            1210..1821
                     /locus_tag="I6J80_RS24815"
                     /old_locus_tag="I6J80_24830"
     CDS             1210..1821
                     /locus_tag="I6J80_RS24815"
                     /old_locus_tag="I6J80_24830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000130481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel transporter"
                     /protein_id="WP_251301786.1"
                     /translation="MTDKTTDIKMALENMIRFRDTIERLGGAMSKELDVETKGKRTYSM
                     NNDMSDLYNVISRAVEASGADHSINEKLTNVLKRELVDYVSIAHLKTKLSVLYEFEKNY
                     LQLIAEYKEEIKFASSLQEDLRKERAKFFSETLKEVHQTLSESQVDNEVASKWIKELVG
                     SYTKSLDLSGGLVEEHTLDTIACIRAEAKLNKPSIEPGNN"
                     /name="nickel transporter"
     gene            1866..2363
                     /locus_tag="I6J80_RS24820"
                     /old_locus_tag="I6J80_24835"
     CDS             1866..2363
                     /locus_tag="I6J80_RS24820"
                     /old_locus_tag="I6J80_24835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000897048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_251301787.1"
                     /translation="MAILERKVNDFKLSSSELPEILAALREARECLVSRSINEDPQLYS
                     ALFLYEKNEGLLSLSFEREYSIFINSNLECILRKVADIKDKKQGKLQKIMNELVRFKYS
                     SSDSCADLLFLSGKLEQIRKIYEVFDRDRNRLEFLNYMMKKERVIFEREFERLLGEKNG
                     DF"
                     /name="hypothetical protein"
     gene            2350..3153
                     /locus_tag="I6J80_RS24825"
                     /old_locus_tag="I6J80_24840"
     CDS             2350..3153
                     /locus_tag="I6J80_RS24825"
                     /old_locus_tag="I6J80_24840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000510993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_204147842.1"
                     /translation="MGIFSQIGSALLNCISKPVDALCEWAKEPLKDREYRREEASKESE
                     HKRNMDVLTAKKRVEHELRMSELNAEYELREKEKTLEVDLEVRRVREIEKAVAEIQEWR
                     KSQELERMERTTAAIARYKEQLTKLNIEVINSIGNMQLDLKERAQKFVYEKAVQYKRLQ
                     DEATEEAMNDLIRIEEKFGDNERAKDILIRAVDTKMGNVIDTSTRFLEELNRDIVSLNQ
                     SIDRLTNQGQKFIESHLERFHIVDATSSLYITSDEKERLLLKNIN"
                     /name="hypothetical protein"
ORIGIN
        1 caggatgtga ttcaggcgct cacagaggcg gggcttgaag tggtcagggt gacgcgaagc
       61 agtatcagca ttgcagatcc gaacggcggg aagaatatca ggctgaaagg agcattttat
      121 gagcaatctt ttgcagacgg gcgcggagtt cgagaaaaag ctgaaagaga gagccgaatc
      181 tacagagaaa atgctgaaca acgagttcag gaggctcggc gaatctgtaa gcgaggctgt
      241 gacatcaaac gagacgaaaa tcagagacgc tatagccctg ttcaccgcct cgacagagga
      301 atcgctggaa aaacaccggg aaggggtgaa agaggcgatg aggcagcaca ggagggacgt
      361 gttaaagctg gcagggaata cgggcatgat gttactgggg atagtctttc tcctgtttac
      421 cgcgagtggc gggacgctct ggtatcttgg cgggaggata caggcgaacc tggaagatat
      481 caggaagcag gaagagacat tgcagaaact gaacgcgaag acatggggcg tggagtttgt
      541 gcaggacggg aacaggaaat ttctggtcct tccttacggg aagtcagcag aggtgatttc
      601 ctttcagggg aaggaatggg tacatctgaa ggagtgacac agagtgacag agctggaaac
      661 acatttgctg aacgccttag agcagctgca acaggactat atgcagcggc tgagcgaatg
      721 ggagagcgcc ttcgtggaat tgcagaagat gttttcgctt acgcaacggg acaacgcgat
      781 gctgaacgag cgggtcatgc agttgagtca gcaggtgcag cacttgagcg agcagacaga
      841 gcgcttgagc cagttataca gcgagaactg gagataagag aggaacggct gatacaggag
      901 cgcgaacatg cgttatccct ggaacgggag cgtcagccgg aaatacagga acgcacgctg
      961 gatggccctt cgctgggatg gtgaaaccat gaaaaatggc agcttcagtg gattaagagg
     1021 gggtaatgtg gcctgtacca tctggttgca taggtattca tacggttaaa atttatcagg
     1081 cgcgatcgcg gctgtttttc gggtggtttg ttgccatttt tacctgtctg ctgccgtgat
     1141 cgcgctgaac gcgtttaagc agtgcgcgta atgcgacgtt atggtaaata gatgagttag
     1201 gcctaagtca tgacagataa aacgaccgat attaaaatgg ctttagaaaa catgatcaga
     1261 tttcgtgaca ctatagagag attggggggg gctatgtcga aagagttgga tgttgagacc
     1321 aaaggaaaac gtacttattc tatgaataac gatatgagtg atttatataa tgtaatatca
     1381 cgggctgttg aagcgtctgg tgccgatcat tcaattaatg aaaaattgac aaatgtcttg
     1441 aaaagagaat tagttgatta tgtcagcatt gcgcatctaa aaaccaaatt gtctgtattg
     1501 tatgagtttg aaaagaatta tttgcagctt atcgcagaat ataaggaaga aataaaattt
     1561 gcttcctctt tgcaagagga tttacgtaaa gaacgtgcta aattcttttc tgagacatta
     1621 aaagaggttc atcaaactct aagtgaatct caagttgata atgaagtggc atcaaaatgg
     1681 attaaagaac ttgttggtag ttataccaaa agcttggatc taagcggagg ccttgttgaa
     1741 gagcatacat tagatacaat tgcttgtatt cgcgctgagg ctaaattaaa taaaccatct
     1801 attgagccag gcaataacta attatggccg ggggtagaca tataaatagt ttgctttatg
     1861 ttcttgtggc tatattagaa cgtaaagtga atgactttaa attgtcttcg tcggaacttc
     1921 ctgagatatt ggctgcttta cgagaggcaa gggaatgcct tgtatcgcgc agcatcaatg
     1981 aagatcctca gttatattcg gcattgtttt tatatgaaaa aaatgaaggg ttgctaagtc
     2041 tttcatttga gagggaatac tctattttta ttaactccaa tttagagtgc atattgagga
     2101 aagttgctga tataaaagat aaaaagcagg gtaaactgca aaaaataatg aatgaacttg
     2161 tgaggtttaa atattcaagt agcgattctt gtgcggatct tttgttttta agtggtaaac
     2221 ttgaacagat acgtaaaata tatgaggtct ttgataggga tagaaataga cttgaatttc
     2281 ttaactatat gatgaagaaa gaacgtgtta tttttgaaag agagtttgaa cggcttttag
     2341 gtgaaaaaaa tggggatttt tagtcagatt ggtagcgctt tgcttaattg tattagtaag
     2401 cctgttgatg cactctgtga atgggctaaa gaaccattga aagataggga atatagaaga
     2461 gaggaggctt caaaggagtc tgaacataag cgcaacatgg atgtcttgac ggccaagaaa
     2521 cgtgttgaac atgaactcag aatgagtgaa ttgaatgctg aatatgagct gagagaaaaa
     2581 gagaaaactc ttgaagttga tctagaggtt cgtcgcgttc gcgaaataga aaaagcagta
     2641 gctgagatcc aggaatggag aaagtcacag gaacttgaga ggatggagcg aacaacagca
     2701 gctattgcac gttacaagga gcaattaacg aaattaaata tagaagtgat taactctatt
     2761 gggaacatgc agcttgattt aaaggagcgc gcacagaaat ttgtctatga gaaagctgta
     2821 caatataagc gcctgcaaga tgaagcaact gaagaggcta tgaatgatct tataaggatt
     2881 gaggaaaaat ttggtgataa tgaaagagct aaagatattt taattcgtgc agttgatact
     2941 aagatgggga atgttattga tacgtcaact cggtttttgg aagaattaaa tagagatatt
     3001 gttagtttaa accaaagtat tgataggctg actaatcaag gacaaaaatt cattgaaagt
     3061 catttggaac gttttcatat tgttgatgcg acatcttctt tatatattac aagtgatgag
     3121 aaagaacgat tgttgctgaa aaatattaat tagtattttg tgagtaatag cgatcattat
     3181 ttttaatggt cgcattgttt gttcttgcgg ttgctaatca ttagtttttg tcttgtggtt
     3241 gaaaactgag cgctatggct atgacctagg agtgggagtg aaaagattca aggatcctct
     3301 tgtgatcctt tttttctgcg cgtaatctgc tgcctgtaaa caaaaaaacc accgctacca
     3361 acggtggttt gtttgccgga atctagagct accaactctt tcttccgaag gtcactggct
     3421 tggaggagcg cagtcaccaa aatctgt
//
