LOCUS       NZ_CP069578             1323 bp    DNA     linear   CON 17-JUN-2023
DEFINITION  Escherichia coli strain FDAARGOS_1250 plasmid unnamed8.
ACCESSION   NZ_CP069578
VERSION     NZ_CP069578.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN16357392
            Assembly: GCF_016890005.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1323)
  AUTHORS   Sokurenko,E., Tallon,L., Sadzewicz,L., Zhao,X., Boylan,J., Ott,S.,
            Bowen,H., Vavikolanu,K., Mehta,A., Aluvathingal,J., Nadendla,S.,
            Yan,Y. and Sichtig,H.
  TITLE     FDA dAtabase for Regulatory Grade micrObial Sequences (FDA-ARGOS):
            Supporting development and validation of Infectious Disease Dx tests
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1323)
  AUTHORS   Sokurenko,E., Tallon,L., Sadzewicz,L., Zhao,X., Boylan,J., Ott,S.,
            Bowen,H., Vavikolanu,K., Mehta,A., Aluvathingal,J., Nadendla,S.,
            Yan,Y. and Sichtig,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2021) Institute for Genome Sciences, University of
            Maryland School of Medicine, BioPark II, 801 W. Baltimore St., Suite
            619, Baltimore, MD 21201, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: SMRT v. 8.0.0, HGAP v. 4, SPAdes v. 3.14.1
            Genome Coverage                   :: 770.24x
            Sequencing Technology             :: Pacbio; Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 06/17/2023 05:03:03
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,029
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,669
            CDSs (with protein)               :: 4,669
            Genes (RNA)                       :: 119
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 241
            CDSs (without protein)            :: 241
            Pseudo Genes (ambiguous residues) :: 0 of 241
            Pseudo Genes (frameshifted)       :: 65 of 241
            Pseudo Genes (incomplete)         :: 178 of 241
            Pseudo Genes (internal stop)      :: 44 of 241
            Pseudo Genes (multiple problems)  :: 45 of 241
            Pseudo Genes (short protein)      :: 4 of 241
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP069578.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..1323
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="FDAARGOS_1250"
                     /host="Homo sapiens"
                     /culture_collection="FDA:FDAARGOS_1250"
                     /db_xref="taxon:562"
                     /plasmid="unnamed8"
                     /geo_loc_name="USA: WA"
                     /collected_by="ARMADA"
     gene            385..861
                     /locus_tag="I6J80_RS24775"
                     /old_locus_tag="I6J80_24790"
     CDS             385..861
                     /locus_tag="I6J80_RS24775"
                     /old_locus_tag="I6J80_24790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000469298.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_000469298.1"
                     /translation="MFIDSEKRLKQLSDEAKKNTEDLEEAKKNSRFTQVSPKGWERVRE
                     LLKDSQGISALKLYSFLAEHIDPTCGAVVADQQFLAEKLGVSRSTIIRWLNYLESKNAL
                     VRIPVAGKVCAYALDPHEVWKGYNTTKNHAAFVTKTLVNKDGDIQRRIMAMFSN"
                     /name="helix-turn-helix domain-containing protein"
     Typing          complement(198..306)
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="98.182"
                     /coverage="100.0"
                     /evalue="4.23e-47"
     Typing          complement(1093..1323)
                     /name="Col(MG828)"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="91.379"
                     /coverage="89.0"
                     /evalue="1.89e-84"
ORIGIN
        1 aacggtgaac ggacggcaaa aacacgatgg agctgttgaa cgtgaaaacc acgaaatctg
       61 gcaaaaatca cggctggcga ggcttgcata ggagtaagcc agtatacact ccgctatcgc
      121 tacgtgactg ggtcagggct tcgccccgac acccccaaag ggcgttgcgt tgcacgcaac
      181 acccttgcct agaatagatc ttttacgccg atttgtaagt ggttgttttt ttagtgtgtt
      241 ttttatttac ctgtcgcatc ctggatgaca caacgttgtc gcatcctgga tgcgacaaca
      301 cgcaacaagg tgttgcacag atgtgtcatg atggcttaga tttgaaccaa cgttagtgca
      361 tgggattttc agagggaaaa aatcatgttt attgattcag aaaaacgact gaaacaactt
      421 tcagatgagg caaagaaaaa caccgaggat ctcgaagaag caaagaaaaa ttcaaggttt
      481 acacaggtat ccccaaaagg ttgggaacgt gttcgagagc tgctgaagga tagccaaggc
      541 atatcagcac tgaagctgta ctcattttta gcggagcata tcgatcctac gtgtggcgct
      601 gtcgttgcgg atcagcaatt cctagctgaa aaacttggag ttagcagaag cacaattatt
      661 cggtggctca attacttaga atcaaaaaat gcattagtta gaatccccgt tgctggtaag
      721 gtttgtgcgt atgccctcga tccacatgaa gtctggaagg gatacaacac tacgaaaaac
      781 catgcagcgt ttgtcactaa aacactggtc aacaaagacg gtgatattca gcgccgaatc
      841 atggccatgt tttcaaattg agctagcggc aggcggacaa tcaggggcta cgtgttaacg
      901 ttctgaccat gattgtctat cctgcattgc tcttttgccg cctcaaaatc ctttgcgtgt
      961 ttttgctccc cgttctccag aaaaaaccga gccgccacgg ttccggcagc gccttgagcg
     1021 ctaccggaaa ccgtctgggg agaaccccag acccccatcg ctgcaacacc gacggtctac
     1081 gaccacctac caaccgcatg gctgcctgtt ccgtgcaaat gggctttgca gggggctggc
     1141 gcggttcctg gcgacctgaa agcccctggt taggggtatc ggcgcgcttg acctcctgag
     1201 agcctctgta agcgtttttc gcgccttcca tgccctggcg ggcatctaaa acccgtttcg
     1261 tgcgtctgag agcttttgag cgcgtttgag gggctatctg gcgactcatc cccaaaagaa
     1321 caa
//
