LOCUS       NZ_CP069576             1902 bp    DNA     circular CON 17-JUN-2023
DEFINITION  Escherichia coli strain FDAARGOS_1250 plasmid unnamed6, complete
            sequence.
ACCESSION   NZ_CP069576
VERSION     NZ_CP069576.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN16357392
            Assembly: GCF_016890005.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1902)
  AUTHORS   Sokurenko,E., Tallon,L., Sadzewicz,L., Zhao,X., Boylan,J., Ott,S.,
            Bowen,H., Vavikolanu,K., Mehta,A., Aluvathingal,J., Nadendla,S.,
            Yan,Y. and Sichtig,H.
  TITLE     FDA dAtabase for Regulatory Grade micrObial Sequences (FDA-ARGOS):
            Supporting development and validation of Infectious Disease Dx tests
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1902)
  AUTHORS   Sokurenko,E., Tallon,L., Sadzewicz,L., Zhao,X., Boylan,J., Ott,S.,
            Bowen,H., Vavikolanu,K., Mehta,A., Aluvathingal,J., Nadendla,S.,
            Yan,Y. and Sichtig,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2021) Institute for Genome Sciences, University of
            Maryland School of Medicine, BioPark II, 801 W. Baltimore St., Suite
            619, Baltimore, MD 21201, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: SMRT v. 8.0.0, HGAP v. 4, SPAdes v. 3.14.1
            Genome Coverage                   :: 770.24x
            Sequencing Technology             :: Pacbio; Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 06/17/2023 05:03:03
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,029
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,669
            CDSs (with protein)               :: 4,669
            Genes (RNA)                       :: 119
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 241
            CDSs (without protein)            :: 241
            Pseudo Genes (ambiguous residues) :: 0 of 241
            Pseudo Genes (frameshifted)       :: 65 of 241
            Pseudo Genes (incomplete)         :: 178 of 241
            Pseudo Genes (internal stop)      :: 44 of 241
            Pseudo Genes (multiple problems)  :: 45 of 241
            Pseudo Genes (short protein)      :: 4 of 241
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP069576.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1902
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="FDAARGOS_1250"
                     /host="Homo sapiens"
                     /culture_collection="FDA:FDAARGOS_1250"
                     /db_xref="taxon:562"
                     /plasmid="unnamed6"
                     /geo_loc_name="USA: WA"
                     /collected_by="ARMADA"
     gene            join(1835..1902,1..220)
                     /locus_tag="I6J80_RS24755"
                     /old_locus_tag="I6J80_24765"
     CDS             join(1835..1902,1..220)
                     /locus_tag="I6J80_RS24755"
                     /old_locus_tag="I6J80_24765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001220925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_049092722.1"
                     /translation="MRIKYESRWRLEELKLEICSSIDELINKYGIEYATGINLYMNCED
                     KEFNLVDVINEDGNKIMFVVSNDEKIQKPKKIAKKKTPAPVIELDKYRKK"
                     /name="hypothetical protein"
     gene            1289..1828
                     /locus_tag="I6J80_RS24760"
                     /old_locus_tag="I6J80_24770"
     CDS             1289..1828
                     /locus_tag="I6J80_RS24760"
                     /old_locus_tag="I6J80_24770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000893324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication/maintenance protein RepL"
                     /protein_id="WP_000893325.1"
                     /translation="MLEQQFEDFEQQKRRDHQVQDQSKNAKQKRDRLNPPDWSQVTKAG
                     WNMLDAISEKVPRAFRLYSFFAQNIDKSCGAVVCDQKFLADYFGVSLRTIQYWIGDLES
                     IGAIVKIPVGIYYAYALDPYQVWRGYNNTKKYAAFNAKTLTQKDGVIDRKLRLMLSRRE
                     EFESSLKYSNHDSEQG"
                     /name="replication/maintenance protein RepL"
     Typing          832..913
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="97.59"
                     /coverage="100.0"
                     /evalue="2.9e-32"
     Typing          complement(459..717)
                     /name="Col(MG828)"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="96.923"
                     /coverage="99.0"
                     /evalue="5.01e-120"
ORIGIN
        1 tgagttaatt aataaatatg gtattgaata tgcaacagga ataaatcttt atatgaattg
       61 tgaagataaa gaatttaatc ttgttgatgt tatcaatgaa gatggaaata aaataatgtt
      121 tgtcgttagc aacgatgaaa aaatacaaaa gccaaaaaaa atagcaaaga aaaaaactcc
      181 agcacctgta attgagctag ataaatatcg aaaaaaatga ccattgagcc agcggcaggc
      241 ggacaatcag gggctacgtg ttaacgttct gaccttgatt gtctatcctg cattgctctt
      301 ttgccgcctc gaatcccttg cgtgtttttg ttccacgctc tccagaacga ccgagccgcc
      361 acggttccga tagcgccttg agcgcaaccg gaaaccgtct ggggagaacc ccagaccccc
      421 atcgctgtaa taccgacggt ctacgaccac ctaccagccg aatagctgcc tatgccgtgc
      481 aaataggctt tgcagggggc tgacgctgtt cctggcgacc tgaaagcccc tggttagggg
      541 tatcggcgcg cctgacctcc tgagagcctc tgtaagcgtt tttcgcgcct tccatgccct
      601 gacgggcatt taaaacccgt ttcgtgcgtc tgagagcttc tgagcgcgtt tgaagggcta
      661 tctgacgact catccctgaa agaacaacaa cgggagcacc gacaacatgc gagaggtgac
      721 accgctcgcc gcaagtcgat gaccgagcgt agcgagcgaa tcgacgagga agcggaagag
      781 aaccggaagc cacattgagc acttacgcac tgatgcgggg tgtcggggtg aagccctgac
      841 caggtggtga atgtctgagt gcgcgtgcgc ggtccgacat tcccacatcc tgtcccgatt
      901 tctgaggctt tttagcggtg aacggacggc aaaaccatga tggagttgtt gaacgttgag
      961 agcatgaaat ctggcaaaaa tcacggctgg taaggcttgc agaggagtaa gccagtatac
     1021 actccgctat cgctacgtga ctgggtcagg gctgcgcccc gacaccccaa aagcagttag
     1081 gtagtgttaa atcatgagca gtaaaaaaac gctttgatag atctttagag caaaaattga
     1141 catttcttta aaaatcattg acttaagaca ccttcatacg aaacgttatt tgcatgtgta
     1201 tacgcaaaca tgtttgcatg tgttgtttcg tactgtacga tataaatttc gtaaggtgcg
     1261 aaatatcatt gcaggtaagg atgcaagtat gttggaacag caatttgaag actttgaaca
     1321 gcaaaaacgg cgagatcatc aggtacaaga tcaatccaag aacgctaagc aaaaacgaga
     1381 tcggttgaat cctccagact ggtcgcaggt tactaaggct ggttggaata tgttggacgc
     1441 tatatcagaa aaagttccta gagcatttcg gttatattct ttttttgccc agaatataga
     1501 taaatcatgt ggggctgttg tatgtgatca aaaattctta gctgattatt tcggtgttag
     1561 tttaagaacg atccaatatt ggattggaga tttagaaagt attggcgcaa tagttaaaat
     1621 accagtaggc atatattatg cctatgcatt agatccttat caggtatggc gtggatataa
     1681 caatactaaa aaatatgcag catttaacgc aaaaacatta actcaaaaag atggggttat
     1741 agatcgtaaa ttgcggctta tgctttctag gcgtgaagag tttgaaagtt ccttaaagta
     1801 ctctaatcat gactcagagc aggggtgatg cactatgaga attaaatatg aatcacgttg
     1861 gagattagaa gaactaaaat tagaaatatg cagttcaata ga
//
