LOCUS       NZ_CP069557             1098 bp    DNA     linear   CON 17-JUN-2023
DEFINITION  Escherichia coli strain FDAARGOS_1253 plasmid unnamed8.
ACCESSION   NZ_CP069557
VERSION     NZ_CP069557.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN16357395
            Assembly: GCF_016889865.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1098)
  AUTHORS   Sokurenko,E., Tallon,L., Sadzewicz,L., Zhao,X., Boylan,J., Ott,S.,
            Bowen,H., Vavikolanu,K., Mehta,A., Aluvathingal,J., Nadendla,S.,
            Yan,Y. and Sichtig,H.
  TITLE     FDA dAtabase for Regulatory Grade micrObial Sequences (FDA-ARGOS):
            Supporting development and validation of Infectious Disease Dx tests
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1098)
  AUTHORS   Sokurenko,E., Tallon,L., Sadzewicz,L., Zhao,X., Boylan,J., Ott,S.,
            Bowen,H., Vavikolanu,K., Mehta,A., Aluvathingal,J., Nadendla,S.,
            Yan,Y. and Sichtig,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2021) Institute for Genome Sciences, University of
            Maryland School of Medicine, BioPark II, 801 W. Baltimore St., Suite
            619, Baltimore, MD 21201, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: SMRT v. 8.0.0, HGAP v. 4, SPAdes v. 3.14.1
            Genome Coverage                   :: 1090.86x
            Sequencing Technology             :: Pacbio; Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 06/17/2023 05:01:47
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,554
            CDSs (total)                      :: 4,441
            Genes (coding)                    :: 4,315
            CDSs (with protein)               :: 4,315
            Genes (RNA)                       :: 113
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 49 of 126
            Pseudo Genes (incomplete)         :: 81 of 126
            Pseudo Genes (internal stop)      :: 27 of 126
            Pseudo Genes (multiple problems)  :: 27 of 126
            Pseudo Genes (short protein)      :: 3 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP069557.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..1098
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="FDAARGOS_1253"
                     /host="Homo sapiens"
                     /culture_collection="FDA:FDAARGOS_1253"
                     /db_xref="taxon:562"
                     /plasmid="unnamed8"
                     /geo_loc_name="USA: WA"
                     /collected_by="ARMADA"
     gene            complement(<1..>1098)
                     /locus_tag="I6J83_RS22555"
                     /old_locus_tag="I6J83_22555"
     CDS             complement(<1..>1098)
                     /locus_tag="I6J83_RS22555"
                     /old_locus_tag="I6J83_22555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016240513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="WP_204084511.1"
                     /translation="VAEAVILTPATAPAWCTNRAELWNAVEKAERRKNSQLAREIELAI
                     PRELPQDAARETVLAFVRENFVSQGMIADVAFHHMDKTNPHAHIMLTTRAVGPAGFGGK
                     VRDWNDRTHAETWRASWADHANRALANAGYQEEIDHRSYERQGLEKTPGIHLGKSACAM
                     ETRGIETERGEQNRLINRLNLEIQISRTELRNSGLTEPARRVADLLNIVLPDNATSYTL
                     RDIIEALPKDSNAAWKLTSNAMSMMADMQATRRRWEELNTERKEAMSHAASLKKSSPFS
                     SGFSRIPLMQWAAPEYRSEQGKIQSLRQQMEKLRQHYRKVEKQDIPASQTAFEKQWQQW
                     GASGLAALRVQLLKREEELRRKEQED"
                     /name="MobA/MobL family protein"
ORIGIN
        1 atcctcctgc tccttccggc gcaattcctc ttcacgttta agcaactgga ctctgagagc
       61 tgcaaggccg gatgcccccc actgctgcca ctgtttttca aatgccgtct gactcgcggg
      121 aatgtcctgt ttctccacct tccggtaatg ctgtcgcaat ttttccattt gctggcgcag
      181 cgactggatt tttccctgtt cgctacggta ttccggtgcc gcccattgca tcaggggaat
      241 acgggaaaaa ccggacgaaa acggcgatga ttttttcagg ctggcagcat ggctcatcgc
      301 ttcctttcgt tcggtgttca gctcttccca gcgtctgcgc gtcgcctgca tatctgccat
      361 catcgacatg gcattactgg tgagtttcca tgccgcatta ctgtccttcg gcaacgcttc
      421 gatgatgtca cgtagtgtgt acgaagttgc gttgtcagga agcacgatat tcagaaggtc
      481 agccacacgg cgggcaggct ctgtcagccc gctgtttctg agttcggtac gggaaatctg
      541 tatttccagg ttaagtctgt taatcagacg attctgttcg ccgcgttctg tttcgatacc
      601 gcgtgtttcc atcgcacagg cagactttcc gaggtgaata ccaggcgttt tctcaagccc
      661 ctgtcgctcg tatgaacgat ggtctatctc ttcctgataa ccggcgttcg ccagtgccct
      721 gttcgcatgg tcagcccatg atgcacgcca cgtctcggca tgtgtccggt cgttccagtc
      781 cctgaccttt ccaccgaatc cagcaggtcc gacagctctc gtggtcagca tgatgtgcgc
      841 atggggattt gttttatcca tgtgatggaa cgccacatca gcaatcatgc cctgactgac
      901 aaagttttct ctgacaaagg cgagaacggt ttcccgtgcg gcatcctgcg gtagttcgcg
      961 ggggatggcg agttcaattt cccgcgcaag ttgtgagttc ttccggcgtt cggccttctc
     1021 aacggcattc cagagttcgg cacggttcgt acaccaggca ggggctgtcg cgggagtcag
     1081 aatcacagct tcagccac
//
