LOCUS       NZ_CP069553             1664 bp    DNA     linear   CON 17-JUN-2023
DEFINITION  Escherichia coli strain FDAARGOS_1253 plasmid unnamed4.
ACCESSION   NZ_CP069553
VERSION     NZ_CP069553.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN16357395
            Assembly: GCF_016889865.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1664)
  AUTHORS   Sokurenko,E., Tallon,L., Sadzewicz,L., Zhao,X., Boylan,J., Ott,S.,
            Bowen,H., Vavikolanu,K., Mehta,A., Aluvathingal,J., Nadendla,S.,
            Yan,Y. and Sichtig,H.
  TITLE     FDA dAtabase for Regulatory Grade micrObial Sequences (FDA-ARGOS):
            Supporting development and validation of Infectious Disease Dx tests
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1664)
  AUTHORS   Sokurenko,E., Tallon,L., Sadzewicz,L., Zhao,X., Boylan,J., Ott,S.,
            Bowen,H., Vavikolanu,K., Mehta,A., Aluvathingal,J., Nadendla,S.,
            Yan,Y. and Sichtig,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2021) Institute for Genome Sciences, University of
            Maryland School of Medicine, BioPark II, 801 W. Baltimore St., Suite
            619, Baltimore, MD 21201, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: SMRT v. 8.0.0, HGAP v. 4, SPAdes v. 3.14.1
            Genome Coverage                   :: 1090.86x
            Sequencing Technology             :: Pacbio; Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 06/17/2023 05:01:47
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,554
            CDSs (total)                      :: 4,441
            Genes (coding)                    :: 4,315
            CDSs (with protein)               :: 4,315
            Genes (RNA)                       :: 113
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 49 of 126
            Pseudo Genes (incomplete)         :: 81 of 126
            Pseudo Genes (internal stop)      :: 27 of 126
            Pseudo Genes (multiple problems)  :: 27 of 126
            Pseudo Genes (short protein)      :: 3 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP069553.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..1664
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="FDAARGOS_1253"
                     /host="Homo sapiens"
                     /culture_collection="FDA:FDAARGOS_1253"
                     /db_xref="taxon:562"
                     /plasmid="unnamed4"
                     /geo_loc_name="USA: WA"
                     /collected_by="ARMADA"
     gene            103..1641
                     /locus_tag="I6J83_RS22500"
                     /old_locus_tag="I6J83_22500"
     CDS             103..1641
                     /locus_tag="I6J83_RS22500"
                     /old_locus_tag="I6J83_22500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998048.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="WP_204084510.1"
                     /translation="MNDISSDDIFLLKQRLAEQEALIHALQEKLSNREREIDHLQAQLD
                     KLRRMNFGSRSEKVSRRIAQMEADLNRLQKESDTLTGRVYDPAVQRPLRQTRTRKPFPE
                     SLPRDEKRLLPAAPCCPNCGGSLSYLGEDTAEQLELMRSAFRVIRTVREKHACTQCDAI
                     VQAPAPSRPIERGIAGPGLLARVLTSKYAEHTPLYRQSEIYGRQGVELSRSLLSGWVDA
                     CCRLLSPLEEALHGYVLTDGKLHADDTPVPVLLPGNKKTKTGRLWTYVRDDRNAGSTLA
                     PAVWFAYSPDRKGIHPQTHLAGFSGVLQADAYAGFNELYRDGRITEAACWAHARRKIHD
                     VHVRTPSALTEEALKRIGELYAIEAEIRGMTAEQRLAERQLKTKPLLKSLESWLREKMK
                     TLSRHSELAKAFAYALNQWPALTYYADDGWAEADNNIAENALRMVSLGRKNYLFFGSDH
                     GGERGALLYSLIGTCKLNGVEPESYLRYVLDVIADWPINRVSELLPWRVALPTE"
                     /name="IS66 family transposase"
ORIGIN
        1 gtatcgactg gcgacagcct aagcggctgc tgacctccct gaccatgctg taaatctctt
       61 tatcctggtt gtcacagaat aagccctgta aaatacgggc ttatgaacga catctcttct
      121 gacgacatct tcctgctgaa acagcgcctg gccgaacagg aagcgctgat ccacgccctg
      181 caggaaaagc tgagcaaccg ggagcgcgaa atagaccatc tgcaggcgca gctggataaa
      241 ctccgccgga tgaacttcgg cagtcgttcc gaaaaagtct cccgccgtat cgcacaaatg
      301 gaagccgatc tgaaccggct tcagaaagag agcgatacgc tgactggtag ggtgtatgac
      361 ccggctgtac agcgtccgtt gcgtcagacc cgcacccgta agccgttccc tgaatcacta
      421 ccccgtgacg aaaagcgact gttgcctgcg gcgccgtgct gcccgaactg cggcggttca
      481 ctgagctatc tgggcgagga taccgccgaa cagctggagt tgatgcgtag cgccttccgg
      541 gttatccgga cggtacggga aaaacatgcc tgtactcagt gcgatgccat cgtgcaggca
      601 cctgcacctt cgcggcccat cgagcggggt atcgccggac cggggctgct ggcccgcgtg
      661 ctgacctcga agtatgcaga gcacaccccg ctgtatcgcc agtcagaaat atacggccgg
      721 caaggtgtgg agctgagccg ttcactgctg tcgggctggg tggatgcatg ctgccggctg
      781 ctgtctccgc tggaagaggc gcttcatggc tatgtgctga ctgacggtaa gctccatgct
      841 gatgacacgc ctgtcccggt gctgttgcca ggcaataaga aaacgaagac cgggcggtta
      901 tggacctacg ttcgtgacga ccgtaacgcc gggtcaacgc tggcgccggc ggtgtggttc
      961 gcttacagcc cggacagaaa aggcatccat ccgcagaccc atcttgcggg gttcagtggt
     1021 gtactgcagg cggatgcata cgccgggttc aacgagctgt accgggatgg ccggataacg
     1081 gaagccgcct gttgggctca cgcccgccgt aaaatccacg atgtgcacgt tcgcaccccg
     1141 tcagccctga cggaggaagc gctgaaacgg atcggcgaac tgtacgccat cgaggcagag
     1201 ataaggggaa tgacggcgga gcagcgcctt gccgaacgtc agttgaaaac gaaaccgctg
     1261 ctgaaatccc tggaaagctg gctgcgtgaa aagatgaaaa ccctgtcgcg acactcagaa
     1321 ctggcgaaag cgttcgcata cgccctgaac cagtggccgg cgctgacgta ctatgcagat
     1381 gatggctggg ctgaggcgga caataacatc gctgaaaatg cgttgcggat ggtcagtctg
     1441 ggccgcaaaa actacctgtt cttcggttcg gatcatggag gagagcgggg agcgctgctg
     1501 tacagcctga tcgggacgtg caaactgaac ggagtggagc cagaaagcta cctccgctat
     1561 gtccttgacg tcatagccga ctggccgata aaccgggtca gcgaactgct cccctggcgc
     1621 gtagcactgc cgactgaata acacatcccc gtcaatacgg ttct
//
