LOCUS       NZ_CP069552             7964 bp    DNA     linear   CON 17-JUN-2023
DEFINITION  Escherichia coli strain FDAARGOS_1253 plasmid unnamed3.
ACCESSION   NZ_CP069552
VERSION     NZ_CP069552.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN16357395
            Assembly: GCF_016889865.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7964)
  AUTHORS   Sokurenko,E., Tallon,L., Sadzewicz,L., Zhao,X., Boylan,J., Ott,S.,
            Bowen,H., Vavikolanu,K., Mehta,A., Aluvathingal,J., Nadendla,S.,
            Yan,Y. and Sichtig,H.
  TITLE     FDA dAtabase for Regulatory Grade micrObial Sequences (FDA-ARGOS):
            Supporting development and validation of Infectious Disease Dx tests
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7964)
  AUTHORS   Sokurenko,E., Tallon,L., Sadzewicz,L., Zhao,X., Boylan,J., Ott,S.,
            Bowen,H., Vavikolanu,K., Mehta,A., Aluvathingal,J., Nadendla,S.,
            Yan,Y. and Sichtig,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2021) Institute for Genome Sciences, University of
            Maryland School of Medicine, BioPark II, 801 W. Baltimore St., Suite
            619, Baltimore, MD 21201, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: SMRT v. 8.0.0, HGAP v. 4, SPAdes v. 3.14.1
            Genome Coverage                   :: 1090.86x
            Sequencing Technology             :: Pacbio; Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 06/17/2023 05:01:47
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,554
            CDSs (total)                      :: 4,441
            Genes (coding)                    :: 4,315
            CDSs (with protein)               :: 4,315
            Genes (RNA)                       :: 113
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 49 of 126
            Pseudo Genes (incomplete)         :: 81 of 126
            Pseudo Genes (internal stop)      :: 27 of 126
            Pseudo Genes (multiple problems)  :: 27 of 126
            Pseudo Genes (short protein)      :: 3 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP069552.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..7964
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="FDAARGOS_1253"
                     /host="Homo sapiens"
                     /culture_collection="FDA:FDAARGOS_1253"
                     /db_xref="taxon:562"
                     /plasmid="unnamed3"
                     /geo_loc_name="USA: WA"
                     /collected_by="ARMADA"
     gene            <1..657
                     /locus_tag="I6J83_RS22450"
                     /old_locus_tag="I6J83_22450"
     CDS             <1..657
                     /locus_tag="I6J83_RS22450"
                     /old_locus_tag="I6J83_22450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004182805.1"
                     /GO_function="GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine radical domain-containing protein"
                     /protein_id="WP_285803658.1"
                     /translation="KIKYTLEQIRDALLANFEGYEGLRRDCLNAPKYGNDDNYVDQYAL
                     DITEWTERECRKYKMLYSTLSHGTLSISNNTPIGELTNATPNGRLAWMPLSDGISPTQG
                     ADKHGPTAIIKSVSKMNVETMNIGMVHNFKFLKGLLDTPEGRHGLITLLRTASILGNGQ
                     MQFSYVDNEVLKKAQQEPEKYRDLIVRVAGYSAYFVELCKEVQDEIISRTVIEKF"
                     /name="glycine radical domain-containing protein"
     gene            709..1656
                     /gene="cutD"
                     /locus_tag="I6J83_RS22455"
                     /old_locus_tag="I6J83_22455"
     CDS             709..1656
                     /gene="cutD"
                     /locus_tag="I6J83_RS22455"
                     /old_locus_tag="I6J83_22455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020316596.1"
                     /GO_function="GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]; GO:1904047 - S-adenosyl-L-methionine
                     binding [Evidence IEA]"
                     /GO_process="GO:0042426 - choline catabolic process
                     [Evidence IEA]; GO:0043687 - post-translational protein
                     modification [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline TMA-lyase-activating enzyme"
                     /protein_id="WP_001275637.1"
                     /translation="MSANKELSGRIFNIQKYSIYDGDGIRTLIFFKGCNLRCPWCANPE
                     GLSSQFQVMFSQDKCINCGDCVNVCPAGIHYRAEVNGEMKHFVNRNKDCIGCRKCEEIC
                     TQNALDIMGKDVTVSELMEIIMQDYDFYVSSGGGVTIGGGEMSLQTDFAVALFSECKKM
                     MINTAVETQGTTPLANYQKLAPVTDTFLFDIKQIDSNHHKTLFGIGNEGVRRNLEWLVD
                     SGANVIVRMPLIRGYNDSWEAITGAIDYVQKLAKRGNIRRIDILPYHQLGRKKYERLEM
                     PYPIVEDPSYSVEELDKLEAFFAQFDFDIRLVRH"
                     /name="cutD"
     gene            1668..2132
                     /locus_tag="I6J83_RS22460"
                     /old_locus_tag="I6J83_22460"
     CDS             1668..2132
                     /locus_tag="I6J83_RS22460"
                     /old_locus_tag="I6J83_22460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001086629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="WP_001086630.1"
                     /translation="MNSLGVIETRGLVAAIQAVDAACKAAGVTCIGYRKVGSGLVTVCF
                     DGEISAVYTAIERGIAVASATDHQAKSLVIARPERCVVEALSNLKGHPPRVESGQKPHA
                     ELPTVVEMAVCEETAEVSTDTETAMPVPEKEVQAPIVEVKSHGHKKGKKA"
                     /name="BMC domain-containing protein"
     gene            2129..2749
                     /locus_tag="I6J83_RS22465"
                     /old_locus_tag="I6J83_22465"
     CDS             2129..2749
                     /locus_tag="I6J83_RS22465"
                     /old_locus_tag="I6J83_22465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000564323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate propanoyltransferase"
                     /protein_id="WP_000564322.1"
                     /translation="MIDALLQEKITTRLIDTAPTIPVGVSNRHVHLSQQDVEALFGVGY
                     QLTPFKPLRQPGQFAAEECVTVVGPKGSLTHVRVLGPVRPTSQLEISRADCFTLGIKAP
                     VRESGQLENAGDALLVGPKGHVELHSQVICAWRHIHMSPQDARLLNVSNGQKVSVRSHG
                     ERQLTFDEVVVRVRNDFALELHIDTEEANAAGLKNGAQVTLIR"
                     /name="phosphate propanoyltransferase"
     gene            2761..3426
                     /locus_tag="I6J83_RS22470"
                     /old_locus_tag="I6J83_22470"
     CDS             2761..3426
                     /locus_tag="I6J83_RS22470"
                     /old_locus_tag="I6J83_22470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000139441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000139438.1"
                     /translation="MTEQQIDTIVNLILQRLQPAVLVMVTSADGYRDLIHQRLARCGER
                     LHLALDETISDSERWQQIGDVIPAKTWQHKLPSTPYKALLLPFLSYPLAVDIVNGTLQS
                     PVAQRVHDALLAGIPVLALRYYCDPHSELNALRGTVHSDYAAHLSATLTGLSECGITLC
                     SMNEMLEKLATDVSSQSPVSHHRRYLTVTDIVNNPALAKSPDAVLTDAAVDFLKAQKK"
                     /name="hypothetical protein"
     gene            3459..3860
                     /locus_tag="I6J83_RS22475"
                     /old_locus_tag="I6J83_22475"
     CDS             3459..3860
                     /locus_tag="I6J83_RS22475"
                     /old_locus_tag="I6J83_22475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012542991.1"
                     /GO_function="GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="WP_000095890.1"
                     /translation="MSSKTKCWLWMLLVILSETSATSTLKMFDNSEGMTKTLLLALIVV
                     LYCICYYSLSRAVKDIPVGLAYATWSGTGILMVSTLGILFYGQHPDTAAIIGMVIIASG
                     IIIMNLFSKMGSEEAEETPVTNLDKKIAN"
                     /name="multidrug efflux SMR transporter"
     gene            3876..4205
                     /locus_tag="I6J83_RS22480"
                     /old_locus_tag="I6J83_22480"
     CDS             3876..4205
                     /locus_tag="I6J83_RS22480"
                     /old_locus_tag="I6J83_22480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011705235.1"
                     /GO_function="GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="WP_000481835.1"
                     /translation="MFNIGFLWLALSIGSEITGTSMIKKTNGFRRLWPSVLVVCAYSTC
                     YFALTRAMSTIPVGVAYSLWCGFGIVGVTIFSMILYKQKPDLPAIFSMLLIISGGIIMN
                     VFSNM"
                     /name="multidrug efflux SMR transporter"
     gene            complement(4429..>4909)
                     /locus_tag="I6J83_RS22485"
                     /old_locus_tag="I6J83_22485"
                     /pseudo=""
     CDS             complement(4429..>4909)
                     /locus_tag="I6J83_RS22485"
                     /old_locus_tag="I6J83_22485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /name="IS3 family transposase"
     gene            complement(4993..6501)
                     /gene="ltrA"
                     /locus_tag="I6J83_RS22490"
                     /old_locus_tag="I6J83_22490"
     CDS             complement(4993..6501)
                     /gene="ltrA"
                     /locus_tag="I6J83_RS22490"
                     /old_locus_tag="I6J83_22490"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708749.1"
                     /GO_function="GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="WP_001189111.1"
                     /translation="MQRKLATWAATDPSLRIQRLLRLITQPEWLAEAARITLSSKGAHT
                     PGVDGVNKTMLQARLAVELQILRDELLSGHYQPLPARRVYIPKSNGKLRPLGIPALRDR
                     IVQRAMLMAMEPIWESDFHTLSYGFRPERSVHHAIRTVKLQLTDCGETRGRWVIEGDLS
                     SYFDTVHHRLLMKAVRRRISDARFMTLLWKTIKAGHIDVGLFRAASEGVPQGGVISPLL
                     SNIMLNEFDQYLHERYLSGKARKDRWYWNNSIQRGRSTAVRENWQWKPAVAYCRYADDF
                     VLIVKGTKAQAEAIREECRGVLEGSLKLRLNMDKTKITHVNDGFIFLGHRIIRKRSRYG
                     EMRVVSTIPQEKARNFAASLTALLSGNYSESKVDMAEQLNRKLKGWAMFYQFVDFKAKV
                     FSYIDRVVFWKLAHWLARKYRTGIASLMRWWCKSPKPGQSKTWVLFGKTNHGKLSGEIL
                     YRLVGQGKKLFRWRLPEGNPYLRTETRNTYTSRFTEVAMAFASI"
                     /name="ltrA"
     gene            complement(<7174..7914)
                     /locus_tag="I6J83_RS22495"
                     /old_locus_tag="I6J83_22495"
                     /pseudo=""
     CDS             complement(<7174..7914)
                     /locus_tag="I6J83_RS22495"
                     /old_locus_tag="I6J83_22495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="frameshifted; incomplete; partial in the middle of a
                     contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /name="IS3 family transposase"
     misc_feature    complement(7518..7634)
                     /locus_tag="I6J83_RS22495"
                     /old_locus_tag="I6J83_22495"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01497"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="AL1L pseudoknot; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /pseudo=""
                     /db_xref="RFAM:RF01497"
     Typing          complement(4433..4761)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="83.784"
                     /coverage="99.0"
                     /evalue="1.7099999999999992e-68"
ORIGIN
        1 aagataaaat atacccttga acagatacgg gacgctctgc tggcaaactt tgaaggttat
       61 gaaggcttgc gtcgcgactg tctgaacgca ccgaaatacg gcaacgacga taactacgtt
      121 gaccagtacg ctctggatat caccgagtgg actgaacgtg aatgccgcaa gtacaagatg
      181 ctctactcca ccctcagcca cggtacgttg tcgatctcca acaatacgcc gattggtgag
      241 ctgaccaatg ccacaccaaa tggtcgtctg gcgtggatgc cgctttctga cgggatcagc
      301 ccgactcaag gggcggataa gcatggccca acggcgatta tcaagtctgt gagcaaaatg
      361 aacgttgaaa ccatgaatat cggcatggtg cataacttca aattcctcaa agggttactt
      421 gatacacctg aaggacgcca cggcctgata actctactgc gtacagcatc aattctcggc
      481 aacggtcaga tgcagttcag ctacgtcgat aacgaagtac tgaaaaaagc acaacaggaa
      541 ccggaaaaat accgtgattt gatcgtccgt gtagccggtt acagcgccta cttcgtggaa
      601 ttgtgcaagg aagttcagga cgaaatcatc agccgtacgg tgattgagaa gttctgataa
      661 gacggatcgc aaccggacaa ggatgtccga cattttcagg aggcaagcgt gagcgcaaat
      721 aaagaattaa gcggacgaat tttcaatatc cagaaatatt cgatctacga cggtgacggt
      781 atccgcacac tgattttttt taaaggctgc aatcttcgct gcccgtggtg tgccaacccg
      841 gaagggttaa gcagccagtt tcaggtgatg ttctctcagg ataaatgcat caactgcggc
      901 gactgcgtca atgtatgccc tgcggggatc cactaccgcg cggaagtcaa cggagaaatg
      961 aagcatttcg tcaaccgtaa taaagactgc attggttgtc gcaagtgtga agaaatttgc
     1021 acccaaaatg cactggatat catgggtaaa gatgtcacgg tcagtgaatt gatggagatc
     1081 atcatgcagg actatgactt ttacgtctcc tccggcggtg gtgtcactat tgggggcggt
     1141 gagatgagct tgcaaactga ttttgccgtt gccctgttca gtgaatgcaa aaagatgatg
     1201 atcaacaccg ccgtcgagac ccagggtaca accccgcttg caaactatca gaagctggct
     1261 ccggtcaccg acaccttcct gttcgatatc aaacagatcg atagcaatca ccataaaacg
     1321 ctgttcggaa ttggcaacga aggcgtgcgt cgcaatctgg agtggctggt ggattcgggg
     1381 gccaacgtga ttgttcgtat gccgctgatt cgtggttata acgattcgtg ggaagcgatt
     1441 accggcgcta tcgactacgt ccagaaactg gccaaacgcg gcaatattcg ccgaatcgat
     1501 atactgccgt accaccagct ggggcgcaaa aagtatgaac gactggagat gccctacccg
     1561 attgttgaag acccgtcata cagcgtggaa gagctggaca aactggaggc gtttttcgcc
     1621 cagttcgatt ttgatattcg cttagttcgt cattaaagga gccgtaaatg aacagtttag
     1681 gcgtaattga aacacgtggt cttgtagccg caatccaggc cgtagatgcc gcctgcaaag
     1741 cagccggggt gacctgtatt ggttatcgca aagtaggttc cgggctggta accgtctgtt
     1801 ttgatgggga aatcagcgca gtttacacgg ccattgaacg tggtattgct gtggcatccg
     1861 caaccgacca tcaggccaaa tcactggtga tcgctcgccc ggaacgctgc gtggttgagg
     1921 ctctcagcaa cctgaaaggt catccgccac gtgtcgaatc aggccagaaa cctcatgcag
     1981 agctgccgac agttgtagaa atggctgtct gcgaggaaac cgctgaagtt tctacagata
     2041 ctgaaactgc aatgcctgta cctgagaaag aagtgcaagc ccccatcgtg gaagtgaaaa
     2101 gccacgggca taagaaaggg aagaaagcat gattgacgca ctgctacagg aaaaaatcac
     2161 cacgcgactg atcgatactg ctcccactat tccggtcggt gtttctaatc gtcatgtgca
     2221 tctgtcgcag caggatgttg aagcactttt tggtgttggc taccaactta cgccctttaa
     2281 gccgttgcgc cagcctgggc aattcgccgc tgaagagtgt gtaacggttg tcggcccgaa
     2341 aggttctctg actcacgtcc gcgtacttgg accggttcgc ccgacaagcc agctggaaat
     2401 ctcacgtgcc gattgtttta ctctcggcat taaagcgcca gtgcgtgaat ccgggcaact
     2461 ggaaaatgcc ggtgacgcac tgttggtggg gccgaaaggt catgttgagt tgcattcaca
     2521 agtcatttgc gcctggcggc atattcatat gtctccccag gatgctcgtc tgttgaatgt
     2581 cagcaatggg cagaaggtga gtgttcgcag tcacggtgaa cgccagttaa cgttcgatga
     2641 agtggtggta cgggtgcgca atgattttgc ccttgaactg catatcgata cggaagaagc
     2701 caatgctgcc ggactgaaaa acggggcgca ggtaacactg attcgctaag ggaggccatc
     2761 atgaccgaac aacagatcga caccatcgtc aacctgatcc tccagcggct gcaacctgcg
     2821 gtactggtca tggtgacatc tgcggatggc tatcgggatc tgattcatca gcggttggct
     2881 cgttgtggcg aacggttgca tctggcgctg gatgaaacaa tcagtgatag cgaacggtgg
     2941 cagcaaattg gtgacgtgat acccgctaaa acatggcaac acaaattgcc ttcaacgccc
     3001 tataaagcgt tattgctgcc gtttttgagt tacccgctgg ctgtagatat tgtcaacggt
     3061 acgctgcaaa gcccggtagc gcaaagggtt cacgatgcgc tactggcggg tattccggta
     3121 ctggcactgc gttattactg cgatccgcac agcgaattaa acgcgcttcg cggtactgtt
     3181 cacagcgact acgcggcgca tctttccgcc acgcttaccg gccttagcga gtgtggcatt
     3241 acgttatgtt ccatgaatga aatgctggaa aaactggcaa cagacgtcag cagtcaatcg
     3301 cctgtaagtc atcatcggcg ttatctgact gtcaccgata tcgtcaataa tccggcactg
     3361 gcaaaatcac ccgacgccgt actgaccgat gctgcggtcg attttttgaa agcacagaaa
     3421 aaataacctt acctcgataa caaacctgga gtttagatat gtcttcgaaa acaaaatgct
     3481 ggctatggat gttactggtc atcctttctg aaacctctgc aacatccaca cttaaaatgt
     3541 tcgataacag tgaggggatg acaaaaacgc tgctgctggc cctaatcgtc gtactgtatt
     3601 gcatttgtta ctactcgctt tcacgggcag taaaagacat ccccgttggt ctggcttacg
     3661 ccacatggtc cggtactggc attttgatgg tttcaaccct tgggatttta ttttacggtc
     3721 aacacccgga taccgccgcc attattggta tggtcatcat agccagcggt attatcatta
     3781 tgaatctgtt ctcaaaaatg ggcagtgaag aggcggaaga aactccagtt accaacctcg
     3841 ataaaaaaat cgctaactga caaaaggaat tgctcatgtt taatattgga tttttatggc
     3901 tggcactttc catcggttct gaaataaccg gtacgtcaat gatcaaaaaa accaatggtt
     3961 ttaggcgact gtggccttct gttctggtgg tatgtgctta ttcaacgtgt tatttcgctc
     4021 tgaccagagc aatgagtact attccggttg gtgttgccta ttcactctgg tgcggctttg
     4081 ggattgttgg cgtgacaatt ttctcgatga ttctttataa acaaaagccc gatttacctg
     4141 ccatattctc catgttgctg attattagtg gcggcattat tatgaatgta ttttcaaaca
     4201 tgtaatgacc ccaaaagtct gctttcacgc acaatgcgga tcagttaagg agcagttgac
     4261 cggtccttaa actgcggcac tatagcggct tccacaacag ggagccgttt tcttgtgcac
     4321 agcccccagc taggctctgt ctcttataca caaatcccca cccggggatt tgtgatgtct
     4381 gtcagttgaa ccgccccggg tttcctggag agtgttttat ctgtgaactc aggctgccag
     4441 ctcatcattt ccgatggaag cataatacgc tttttctgct tctgccggag ggatatggcc
     4501 cagcctttcc agcaatcatc gattgttata ccagtccacc cacgtgagtg tggccagttc
     4561 cacttctgca cggtttttcc agctcttacg gtgtattacc tccgctttgt aaagaccatt
     4621 gatgctctcc gccatcgcgt tgtcatacga gtcgcctgta cttcctgttg atgccagtaa
     4681 tccggcttcc ttaagccgct gtgtgtaggc cagcgataca tactgagagc ctttatcact
     4741 gtgatggacc tgtgccggac ggtcgacggg cccataacgc ctgctccagt gcatccagca
     4801 cgaatgtcgt ttccatggac gatgagaccc gccaccccac gatgtatccg gcaaacacat
     4861 caatgatgaa cgccacatag acgaagccct gccatgtgct gagtagagta agaggcaggg
     4921 cgtagtcgga ctatttccct gcctctcctc cccgaaccgg acgtgcacct ttcagcgcat
     4981 ccggctctcc atttaaatgc tggcgaacgc cattgccact tctgtaaagc gcgatgtata
     5041 cgtgtttctg gtctccgtcc tcagataggg attaccttcg ggtagccgcc agcggaacag
     5101 cttcttgcct tgccccacca accggtacag tatttcgccg ctgagcttgc cgtgattggt
     5161 tttaccaaat aaaacccacg ttttgctctg acccggtttc ggtgatttac accaccacct
     5221 catcagggaa gcgatacctg tacggtattt gcgggccagc cagtgagcca gcttccagaa
     5281 cacgacacgg tcgatataac tgaagacttt ggccttaaaa tcaacgaact gatagaacat
     5341 ggcccagcct ttcagttttc ggttgagttg ttcagccata tcgactttgc tttcactgta
     5401 gttgcctgat aacagtgctg tcagcgatgc ggcgaagttt ctggctttct cctgcgggat
     5461 cgttgagacc actcgcatct cgccataacg actgcgtttg cgaatgatcc tgtgccccag
     5521 aaagataaag ccgtcattaa catgggtgat tttagtctta tccatgttca gcctgagttt
     5581 cagactgcct tcgagcacac cccgacactc ctccctgatg gcttccgcct gtgctttggt
     5641 gcctttgacg atgaggacaa aatcatcggc atagcggcag tacgccaccg cgggtttcca
     5701 ctgccagttt tctctgaccg ccgtacttcg gccccgttgg atactgttat tccagtacca
     5761 ccgatctttt ctggctttcc cgctcaggta gcgctcatgc aggtattgat cgaactcatt
     5821 cagcatgatg ttcgataata gcggcgatat aacaccgccc tgtggcacac cttcactggc
     5881 cgcccgaaag agaccgacat cgatatgtcc cgccttgatg gttttccaca gcagagtcat
     5941 gaaacgtgcg tcactgatcc tgcggcgtac agccttcatc agcagtcgat gatgtacggt
     6001 gtcgaagtaa ctggacaggt cgccttcaat cacccagcgt ccccgggttt caccacagtc
     6061 tgtgagctgt aatttcaccg tgcggatcgc gtggtggaca ctgcgctcag gccggaagcc
     6121 atatgagagc gtatgaaaat cactctccca tatcggctcc atcgccatca gcatggcccg
     6181 ctgaacaata cgatcccgca acgcggggat acccagtggt cgcagtttgc cgttgctttt
     6241 agggatgtaa acccgtctgg cgggcaaggg ctggtagtgg cctgagagta attcatccct
     6301 gaggatttgc agctcaacag ccagtctggc ctgtagcatt gttttgttca cgccatcaac
     6361 gccgggggta tgggccccct ttgatgaaag cgtgatccgc gccgcttcag ccagccattc
     6421 tggttgtgtt atcagacgca gcagccgttg aatccgtagg gacggatcgg tggctgccca
     6481 tgtggcaagc ttgcgttgca tttcgctgat tatcaaaggt cttcacctcg ttaggtcagt
     6541 taattcacgt cgcaaacaca ttcaaactgc ttcccttcgc catgtaatgg gctttcccca
     6601 tcgcggacta ctacggaagc tccgccagcc agcgcgtcat cggagccatg cccccttaac
     6661 atccgtcgct gaccttcccc ggtttacctg cctggactca ggcatactga ggaggctgcc
     6721 cgtcgcactc tttatccttg cttgccgcaa gttggcagaa gtcagcaacg caagcgtgat
     6781 agacgctgct gccccggtgt ttcgcataca tgtcaaaaca ccttcgaccg gtagtgctta
     6841 cgtatcactg ccagttcctc ctgcacggcc tgtcagatca cgtaggccgt ggtgacgttt
     6901 tcaacccaca gaggcggatt aacgggttca tgttcttcag cctttcagta cttaaccttg
     6961 aggatcatct cggcttagtg atctcgcctc aatccccgtt gtcagcgggt tacatcaccc
     7021 tgcgggcatg ccgcaggtca ctgccgctca ggttctccac cgtcacaccc ggtgggattg
     7081 ttgggtttct catcgtgagt taccggttca atattccaga cagactcgcg gttcatttaa
     7141 gcatccatgc ccgccctgaa ctccgggcac accgtaagta aaatcagcca cccacaactg
     7201 gtcaggacgt tctgccacaa actgacggtt tacgcggtcg cctgcggcaa cggctttccg
     7261 gctgacggta gtgcggacct ttttaccccg gagaacaccg gcaagtccca taaccgccat
     7321 gaggcgcgcc actgtacatc tggcctaccc tgattccttc ccgtaacaac tgacgccaga
     7381 ctttacgcac accgtatacc tgatgatttc catcgtatac gcgcatgtat ctctttcttc
     7441 agccagtcat cgcgctgcgc acgggcactg cgtttatcag gatgatgtcg ctgttgctga
     7501 cagtggtaat acgttgacgg ggcaatatgc agttcactgc ataccggtcc gaccccgtac
     7561 tgctcacgca gcttatccag cagtggcatc actttttcca gaggcggtcg aactccgcct
     7621 tcgcaaaata agcggaagcc tggcgaagga tatcgttact gcggcgcagt tcacgatttt
     7681 cacgttccag ctctttcaga cgctgacgtt cagcggtggt gagcccgcca tcaccgcctc
     7741 cggtatcccg ctcatgctgg cgtacccaga cacgcagagt ctccggtgta cagccaatct
     7801 ttgggcaatg gaacaaattg ccgcccattg tgagtcatat tcgccctgac tttccagaac
     7861 catacgaact gcccgttgac ggacttcggg ggaaaaacgt gtatttttag tcatcctgtt
     7921 tacctctttc tcaggaagtt tagtctccag gattttccgg ggcg
//
