LOCUS       NZ_CP069551            15597 bp    DNA     linear   CON 17-JUN-2023
DEFINITION  Escherichia coli strain FDAARGOS_1253 plasmid unnamed2.
ACCESSION   NZ_CP069551
VERSION     NZ_CP069551.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN16357395
            Assembly: GCF_016889865.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15597)
  AUTHORS   Sokurenko,E., Tallon,L., Sadzewicz,L., Zhao,X., Boylan,J., Ott,S.,
            Bowen,H., Vavikolanu,K., Mehta,A., Aluvathingal,J., Nadendla,S.,
            Yan,Y. and Sichtig,H.
  TITLE     FDA dAtabase for Regulatory Grade micrObial Sequences (FDA-ARGOS):
            Supporting development and validation of Infectious Disease Dx tests
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 15597)
  AUTHORS   Sokurenko,E., Tallon,L., Sadzewicz,L., Zhao,X., Boylan,J., Ott,S.,
            Bowen,H., Vavikolanu,K., Mehta,A., Aluvathingal,J., Nadendla,S.,
            Yan,Y. and Sichtig,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2021) Institute for Genome Sciences, University of
            Maryland School of Medicine, BioPark II, 801 W. Baltimore St., Suite
            619, Baltimore, MD 21201, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: SMRT v. 8.0.0, HGAP v. 4, SPAdes v. 3.14.1
            Genome Coverage                   :: 1090.86x
            Sequencing Technology             :: Pacbio; Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 06/17/2023 05:01:47
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,554
            CDSs (total)                      :: 4,441
            Genes (coding)                    :: 4,315
            CDSs (with protein)               :: 4,315
            Genes (RNA)                       :: 113
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 49 of 126
            Pseudo Genes (incomplete)         :: 81 of 126
            Pseudo Genes (internal stop)      :: 27 of 126
            Pseudo Genes (multiple problems)  :: 27 of 126
            Pseudo Genes (short protein)      :: 3 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP069551.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..15597
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="FDAARGOS_1253"
                     /host="Homo sapiens"
                     /culture_collection="FDA:FDAARGOS_1253"
                     /db_xref="taxon:562"
                     /plasmid="unnamed2"
                     /geo_loc_name="USA: WA"
                     /collected_by="ARMADA"
     gene            <1..579
                     /gene="traJ"
                     /locus_tag="I6J83_RS22330"
                     /old_locus_tag="I6J83_22330"
     CDS             <1..579
                     /gene="traJ"
                     /locus_tag="I6J83_RS22330"
                     /old_locus_tag="I6J83_22330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003979562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer transcriptional regulator TraJ"
                     /protein_id="WP_204084505.1"
                     /translation="TGKFIFCNTLFHESFLTQDQSAEKWLLSQRDFYELISVTEMEAYR
                     NEHTHLNLVEDIFIQNRFWTISVQSFLNGHRNIILWQFYDAAHVRHKDSYNQKTIVSDD
                     IRNIIRRMSDDSSVSSYVNDVFYLYSTGISHNAIARILNISISTSKKHASLICDYFSVS
                     NKDELIILLYNKKFIHYLYEKAMCIINMR"
                     /name="traJ"
     gene            672..1073
                     /gene="traY"
                     /locus_tag="I6J83_RS22335"
                     /old_locus_tag="I6J83_22335"
     CDS             672..1073
                     /gene="traY"
                     /locus_tag="I6J83_RS22335"
                     /old_locus_tag="I6J83_22335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012006553.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer relaxosome DNA-bindin protein
                     TraY"
                     /protein_id="WP_001369361.1"
                     /translation="MLLKRFGTRSATGKMVKLKLPVDVESLLIEASNRSGRSRSFEAVI
                     RLKDHLHRYPKFNRAGNIYGKSLVKYLTMRLDDETNQLLIAAKNRSGWCKTDEAADRVI
                     DHLIKFPDFYNSEIFREADKEEDITFNTL"
                     /name="traY"
     gene            1106..1464
                     /gene="traA"
                     /locus_tag="I6J83_RS22340"
                     /old_locus_tag="I6J83_22340"
                     /pseudo=""
     CDS             1106..1464
                     /gene="traA"
                     /locus_tag="I6J83_RS22340"
                     /old_locus_tag="I6J83_22340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001673167.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system pilin TraA"
                     /name="traA"
     gene            1479..1790
                     /gene="traL"
                     /locus_tag="I6J83_RS22345"
                     /old_locus_tag="I6J83_22345"
     CDS             1479..1790
                     /gene="traL"
                     /locus_tag="I6J83_RS22345"
                     /old_locus_tag="I6J83_22345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system protein TraL"
                     /protein_id="WP_000012100.1"
                     /translation="MSGDENKLKKYRFPETLTNQSRWFGLPLDELIPAAICIGWGIITS
                     KYLFGIGAAVLVYFGIKKLKKGRGSSWLRDLIYWYMPTALLRGIFHNVPDSCFRQWIK"
                     /name="traL"
     gene            1812..2378
                     /gene="traE"
                     /locus_tag="I6J83_RS22350"
                     /old_locus_tag="I6J83_22350"
                     /pseudo=""
     CDS             1812..2378
                     /gene="traE"
                     /locus_tag="I6J83_RS22350"
                     /old_locus_tag="I6J83_22350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490565.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system protein TraE"
                     /name="traE"
     gene            2365..3093
                     /gene="traK"
                     /locus_tag="I6J83_RS22355"
                     /old_locus_tag="I6J83_22355"
     CDS             2365..3093
                     /gene="traK"
                     /locus_tag="I6J83_RS22355"
                     /old_locus_tag="I6J83_22355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system secretin TraK"
                     /protein_id="WP_001230767.1"
                     /translation="MRKNNTAIIFGGLFFSCSVMAANGTLAPAVVPMVNGGQASIAISN
                     TSPNLFTVPGDRIIAVNSLDGALTNNEQTASGGVVVATVNKKPFTFILETERGLNLSIQ
                     AVPREGAGRTIQLVSDLRGTGEEAGAWETSTPYESLLVTISQAVRGGKLPAGWYQVPVT
                     KETLQAPAGLSSVADSVWTGNHLKMVRFVVENKTLSALNIRESDFWQPGTRAVMFSQPA
                     SQLLAGARMDVYVIRDGEGN"
                     /name="traK"
     gene            3093..4544
                     /gene="traB"
                     /locus_tag="I6J83_RS22360"
                     /old_locus_tag="I6J83_22360"
     CDS             3093..4544
                     /gene="traB"
                     /locus_tag="I6J83_RS22360"
                     /old_locus_tag="I6J83_22360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000146653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F-type conjugal transfer pilus assembly protein
                     TraB"
                     /protein_id="WP_000146663.1"
                     /translation="MASINTIVKRKQYLWLGIVVVGAASAIGGVLYLSDVDMSGNGEAV
                     AEQEPVPDMTGVVDTTFDDKVRQHATTEMQVTAAQMQKQYEEIRRELDVLNKQRGDDQR
                     RIEKLGQDNAALAEQVKALGANPVTATGEPVPQMPASPPGPEGEPQPGNTPVSFPPQGS
                     VAVPPPTAFYPGNGVTPPPQVTYQSVPVPNRIQRKVFTRNEGKQGPSLPYIPSGSFAKA
                     MLIEGADANASVTGNESTVPMQLRITGLVEMPNSKTYDATGCFVGLEAWGDVSSERAIV
                     RTRNISCLKDGKTIDMPIKGHVSFRGKNGIKGEVVMRNGKILGWAWGAGFVDGIGQGME
                     RASQPAVGLGATAAYGAGDVLKMGIGGGASKAAQTLSDYYIKRAEQYHPVIPIGAGNEV
                     TVVFQDGFQLKTVEEMALEQSQSTAEEGNPESPVPNPPSAESHLNGFNTDQMLKQLGNL
                     NPQQFMSGDKNPLGGDAGKEGGNGG"
                     /name="traB"
     gene            4534..5100
                     /gene="traP"
                     /locus_tag="I6J83_RS22365"
                     /old_locus_tag="I6J83_22365"
     CDS             4534..5100
                     /gene="traP"
                     /locus_tag="I6J83_RS22365"
                     /old_locus_tag="I6J83_22365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000896598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus-stabilizing protein TraP"
                     /protein_id="WP_251302545.1"
                     /translation="MAGDALTFRVAFVVRWLLWVVRFVVIWPLATMALMALFVLWKDNT
                     TPGKLLVKEINFVRQTAPAGQFPVSECWFSSSDSSGRSEIQDICHYRAADAADYVRETD
                     RSLMQLVTALWATLALMYMSFAAGTGKYPVRPGKMKCIRVVTADEHLKEVYTEDASLPG
                     KIRKCPVYLPDDRTNRNNGDKNEHA"
                     /name="traP"
     gene            5087..5404
                     /locus_tag="I6J83_RS22370"
                     /old_locus_tag="I6J83_22370"
     CDS             5087..5404
                     /locus_tag="I6J83_RS22370"
                     /old_locus_tag="I6J83_22370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001057281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbD"
                     /protein_id="WP_001057297.1"
                     /translation="MNMRNINVITARAVPDKNVSDDFMHAVLSNCTTRIALPAPKKFSS
                     ESLPHNFNMAAVGVMKKGMSMSLFSSPGNTVVSGQPCAGKTNLTVELISAVVESNKKGE
                     G"
                     /name="conjugal transfer protein TrbD"
     gene            5404..5919
                     /gene="traV"
                     /locus_tag="I6J83_RS22375"
                     /old_locus_tag="I6J83_22375"
     CDS             5404..5919
                     /gene="traV"
                     /locus_tag="I6J83_RS22375"
                     /old_locus_tag="I6J83_22375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system lipoprotein
                     TraV"
                     /protein_id="WP_000809872.1"
                     /translation="MKQISLFIPLLGTLLLSGCAGTNSDFECDATTSDTCMTMEQANEK
                     AKKLEQSSEAKPVAASLPRLAEGNFRTTSVQAVAATTPSRSRPAVTAHPEQKLLAPRPL
                     FTAAREVKTVVPVSSVAPVTPPRPLRTGEQTAALWIAPYIDNQDVYHQPSSVFFVIKPS
                     AWGKPRIN"
                     /name="traV"
     gene            6054..6275
                     /gene="traR"
                     /locus_tag="I6J83_RS22380"
                     /old_locus_tag="I6J83_22380"
     CDS             6054..6275
                     /gene="traR"
                     /locus_tag="I6J83_RS22380"
                     /old_locus_tag="I6J83_22380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraR"
                     /protein_id="WP_001278695.1"
                     /translation="MSDEADEAYSVTEQLTMTGINRIRQKINVHGIPVYLCEACGNPIP
                     EARRKIFPGVTLCVECQAYQERQRKHYA"
                     /name="traR"
     gene            6268..6681
                     /locus_tag="I6J83_RS22385"
                     /old_locus_tag="I6J83_22385"
     CDS             6268..6681
                     /locus_tag="I6J83_RS22385"
                     /old_locus_tag="I6J83_22385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000549587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000549574.1"
                     /translation="MHKSENEKIDFILDEHEQIFRKQGSLCMALGKGFLVIAILCGVAT
                     FCVSGLYLKSLCLSVACCCGVFSRIFNYYSVPEESRMVLSRQELLRLMRLTEDVPDVRQ
                     ELLNRLLSGKKLTGRDEQDIRRLWQETQRKHYA"
                     /name="hypothetical protein"
     gene            6674..7147
                     /locus_tag="I6J83_RS22390"
                     /old_locus_tag="I6J83_22390"
     CDS             6674..7147
                     /locus_tag="I6J83_RS22390"
                     /old_locus_tag="I6J83_22390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000549572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000549566.1"
                     /translation="MHKSEAEKIDSILDEHQQIYRRHNQINNILAYCACIAWIPCIIAA
                     FGVDGIYLKILFAVLACCGAVCFFIFFFTLLPESLMVLSRQSLLQLMRLTEDVPDARQE
                     LLNRLLSGKKLTGRDEQDIRRLWQEKVDAMQESATRQREQDTIRKFTEGNKSE"
                     /name="hypothetical protein"
     gene            7227..7445
                     /locus_tag="I6J83_RS22820"
     CDS             7227..7445
                     /locus_tag="I6J83_RS22820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000556745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000556746.1"
                     /translation="MHTFIFLVLIKNINGGGSWNITPMPNTDVCVQVLRTIESHGGRGF
                     GNTGECIEVKTNKPLNNVMDTLNGGGQ"
                     /name="hypothetical protein"
     gene            7984..>8586
                     /locus_tag="I6J83_RS22825"
                     /pseudo=""
     CDS             7984..>8586
                     /locus_tag="I6J83_RS22825"
                     /inference="COORDINATES: protein motif:HMM:NF022580.2"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="TraC family protein"
                     /name="TraC family protein"
     gene            8665..9135
                     /locus_tag="I6J83_RS22830"
     CDS             8665..9135
                     /locus_tag="I6J83_RS22830"
                     /inference="COORDINATES: protein motif:HMM:NF039299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5934 domain-containing protein"
                     /protein_id="WP_251302548.1"
                     /translation="MADNYSNLLNPELSISCPFILTLTLVVEDQVKTHSEANLKYMDLE
                     KKSKTSYAKWFPSVEKEAKEWGELRQRLGSGTVFCRVLLPQHHSLLQGQLMKRQLEVEQ
                     DILNSFRKNGFELISPRFNHMRNFLTCLPFMAGKGLFKQLKEAGCGSARREL"
                     /name="DUF5934 domain-containing protein"
     gene            9110..10036
                     /locus_tag="I6J83_RS22835"
     CDS             9110..10036
                     /locus_tag="I6J83_RS22835"
                     /inference="COORDINATES: protein motif:HMM:NF040179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="WP_251302546.1"
                     /translation="MVQRAESFNVANLMPLVADNPLTPAGLLAPTYRNQLAFIDIFFQG
                     MNNTNYNMAVCGTSGAGKTGLIQPLIRSVLDSGGFAVVFDMGDGYKSLCENMGGVYLDG
                     ETLRFNPFANITDIDQSAERVRDQLSVMASPNGNLDEVHEGLLLQAVRASWLAKENRAR
                     IDDVVDFLKNASDSEQYAGSPTIRSRLDEMIVLLDQYTANGTYGQYFNSDEPSLRDDAK
                     MVVLELGGLEDRPSLLVAVMFSLIIYIENRMYRTPRTLKKLNVIDEGWRLLDFKNRKVG
                     EFIQKGYRTCRRPYRCLYHHHTEHRRL"
                     /name="DUF87 domain-containing protein"
     gene            9951..10451
                     /locus_tag="I6J83_RS22840"
     CDS             9951..10451
                     /locus_tag="I6J83_RS22840"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_251302547.1"
                     /translation="MSLFKKVTAPVAVHTGAYITITQNIVDFDSDKASSAARAAWGNSS
                     YKIILKQSAKEFAKYNQLFPDQFQPLQRDMIGKFGAAKDQWFSSFLLQVENHSSWHRLF
                     VDPLSRAMYSSDGPDFEFVQQKRKEGLSIHEAVWQLAWKKSGPEMASLEAWLEEHEKYR
                     SVA"
                     /name="hypothetical protein"
     gene            10448..10834
                     /gene="trbI"
                     /locus_tag="I6J83_RS22405"
                     /old_locus_tag="I6J83_22405"
     CDS             10448..10834
                     /gene="trbI"
                     /locus_tag="I6J83_RS22405"
                     /old_locus_tag="I6J83_22405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system protein TrbI"
                     /protein_id="WP_000214084.1"
                     /translation="MTTTQKTTDVTAPRRSHWWWTVPGCLAMVLLNAAVSYGIVRLNAP
                     VTAAFNMKQTVDAFFDSASQKQLSEAQSKALSARFNTALEASLQAWQQKHHAVILVSPA
                     VVQGAPDITREIQQDIARRMRAEP"
                     /name="trbI"
     gene            10831..11463
                     /gene="traW"
                     /locus_tag="I6J83_RS22410"
                     /old_locus_tag="I6J83_22410"
     CDS             10831..11463
                     /gene="traW"
                     /locus_tag="I6J83_RS22410"
                     /old_locus_tag="I6J83_22410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system protein TraW"
                     /protein_id="WP_001203728.1"
                     /translation="MRCRGLIALLIWGQSVAAADLGTWGDLWPVKEPDMLTVIMQRLTA
                     LEQSGEMGRKMDAFKERVIRNSLRPPAVPGIGRTEKYSSRLFDPSVRLAADIRDNEGRV
                     FARQGEVMNPLQYVPFNQTLYFINGDDPAQVAWMKRQTPPTLESKIILVQGSIPEMQKS
                     LDSRVYFDQNGVLCQRLGIDQVPARVSAVPGDRFLKVEFIPAEEGRK"
                     /name="traW"
     gene            11460..12452
                     /gene="traU"
                     /locus_tag="I6J83_RS22415"
                     /old_locus_tag="I6J83_22415"
     CDS             11460..12452
                     /gene="traU"
                     /locus_tag="I6J83_RS22415"
                     /old_locus_tag="I6J83_22415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus assembly protein TraU"
                     /protein_id="WP_000830838.1"
                     /translation="MKRSLWLLMLFLLAGHVPAASADSACEGRFVNPITDICWSCIFPL
                     SLGSIKVSQGKVPDTANPSMPIQICPAPPPLFRRIGLAIGYWEPMALTDVTRSPGCMVN
                     LGFSLPAFGKTAQGTAKKDEKQVNGAFYHVHWYKYPLTYWLNIITSLGCLEGGDLDIAY
                     LSEIDPTWTDSSLTTILNPEAVIFANPIAQGACAADAIASAFNMPLDVLFWCAGSQGSM
                     YPFNGWVSNESSPLQSSLLVSERMAFKLHRQGMIMETIGKNNAVCNEYPSPILPKERWR
                     YQMVNMYPDSGQCHPFGRSVMRWETGKNPPNTKKNFGYLMWRKRNCVFL"
                     /name="traU"
     gene            12479..12775
                     /locus_tag="I6J83_RS22420"
                     /old_locus_tag="I6J83_22420"
     CDS             12479..12775
                     /locus_tag="I6J83_RS22420"
                     /old_locus_tag="I6J83_22420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001365600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_204084509.1"
                     /translation="MEKKLSSKYIHMYMSISRVFIRLTGNVLKLFPFAMVICPVVWFYL
                     CPEHIISDIVNDWQDADTLEKILLVKLYLKTVLILTVVSVVVLSLSSDKFMSC"
                     /name="hypothetical protein"
     gene            12851..13369
                     /locus_tag="I6J83_RS22425"
                     /old_locus_tag="I6J83_22425"
     CDS             12851..13369
                     /locus_tag="I6J83_RS22425"
                     /old_locus_tag="I6J83_22425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001473201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000009088.1"
                     /translation="MSFLSFLRKISNKEKEIFISEERFFSLLERVSAFDVVREWAENGG
                     AYYPLLECRRLTDIEDVRKSEDILNRLEFIDRRFREFISIMSEYNRNDKSKNRWERELS
                     EYPPEKIYQVRREGNHLSLKNYGDDITLNRDTAVATVQITVSRKCITQEEMDELYALSE
                     RLKEKCAGN"
                     /name="hypothetical protein"
     gene            13396..14034
                     /gene="trbC"
                     /locus_tag="I6J83_RS22430"
                     /old_locus_tag="I6J83_22430"
     CDS             13396..14034
                     /gene="trbC"
                     /locus_tag="I6J83_RS22430"
                     /old_locus_tag="I6J83_22430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001473203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin assembly
                     protein TrbC"
                     /protein_id="WP_204084508.1"
                     /translation="MSRKIFVLFALTTGLFISAHASENVNTPENRQFLKQQETLSQQLR
                     EKPDTQLQAWAEQQVRANPLQHSDNQFLDELVRKQQAAQKDKPQQGAVYFVSFSIPEEG
                     LKRMLGETRHYGIPATLRGMVNNDLKTTAEAVLSLVKDGATDGVQIDPTLFSQYGIRSV
                     PALVVFCSQGHDIIRGNLRVRQALEKVAAAGDCRQVARALLNNPGDKQK"
                     /name="trbC"
     gene            14031..14402
                     /locus_tag="I6J83_RS22435"
                     /old_locus_tag="I6J83_22435"
     CDS             14031..14402
                     /locus_tag="I6J83_RS22435"
                     /old_locus_tag="I6J83_22435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000056340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000174465.1"
                     /translation="MTLPAGYYRIDPDIRALVAAMNVHGFRTYASCQGHGFPVTKLPPY
                     IAFVCPVKKAALLEQRLRQDAESMMPRLLWGWSVGASFNSDLQLCFRLQPEGPHHWYHR
                     YCRRSLRADFRTLVRLLNP"
                     /name="hypothetical protein"
     gene            14427..14850
                     /locus_tag="I6J83_RS22440"
                     /old_locus_tag="I6J83_22440"
                     /pseudo=""
     CDS             14427..14850
                     /locus_tag="I6J83_RS22440"
                     /old_locus_tag="I6J83_22440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003982281.1"
                     /GO_function="GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /name="HNH endonuclease signature motif containing protein"
     gene            14847..>15597
                     /locus_tag="I6J83_RS22445"
                     /old_locus_tag="I6J83_22445"
                     /pseudo=""
     CDS             14847..>15597
                     /locus_tag="I6J83_RS22445"
                     /old_locus_tag="I6J83_22445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001403430.1"
                     /note="TraN; TrhN; involved in stabilizing mating pairs
                     during plasmid conjugation; F plasmid TraN appears to
                     recognize OmpA in the recipient cell; incomplete; too short
                     partial abutting assembly gap; missing C-terminus; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraN"
                     /name="conjugal transfer protein TraN"
     Typing          1480..1787
                     /name="MPF_F"
                     /typing="mate-pair-formation"
                     /tool="MOB-suite"
                     /identity="99.029"
                     /coverage="99.0"
                     /evalue="6.02e-63"
     Typing          2366..3090
                     /name="MPF_F"
                     /typing="mate-pair-formation"
                     /tool="MOB-suite"
                     /identity="97.521"
                     /coverage="100.0"
                     /evalue="2.59e-154"
     Typing          3094..4502
                     /name="MPF_F"
                     /typing="mate-pair-formation"
                     /tool="MOB-suite"
                     /identity="96.815"
                     /coverage="97.0"
                     /evalue="0.0"
     Typing          5405..5916
                     /name="MPF_F"
                     /typing="mate-pair-formation"
                     /tool="MOB-suite"
                     /identity="92.982"
                     /coverage="100.0"
                     /evalue="8.56e-93"
     Typing          10832..11460
                     /name="MPF_F"
                     /typing="mate-pair-formation"
                     /tool="MOB-suite"
                     /identity="99.048"
                     /coverage="97.0"
                     /evalue="3.61e-135"
     Typing          11461..12416
                     /name="MPF_F"
                     /typing="mate-pair-formation"
                     /tool="MOB-suite"
                     /identity="98.746"
                     /coverage="83.0"
                     /evalue="0.0"
     Typing          13397..14025
                     /name="MPF_F"
                     /typing="mate-pair-formation"
                     /tool="MOB-suite"
                     /identity="90.0"
                     /coverage="99.0"
                     /evalue="9.95e-126"
ORIGIN
        1 actggaaaat ttattttttg caatacgctg tttcatgagt catttcttac acaagatcaa
       61 agtgctgaaa aatggcttct gtcgcagaga gatttttatg aattgatctc tgtcacagag
      121 atggaagcat acaggaatga gcatacgcat cttaatcttg tagaggatat ttttattcag
      181 aacagattct ggacaatatc tgtccagtca tttcttaatg gacacagaaa tattattctg
      241 tggcaatttt atgatgctgc tcatgttcgt cataaagaca gttataatca aaaaacgatt
      301 gtcagtgatg atatcagaaa tataatcaga agaatgagtg atgattcttc tgtatcatca
      361 tatgtaaatg atgtatttta cttatatagc accggaatca gtcataatgc tatagcaaga
      421 atattaaaca tatccatctc cacatcaaag aaacacgcat ctctgatatg cgactacttc
      481 tctgtttcta ataaagatga gttaatcatc ttactctaca ataaaaagtt tattcattat
      541 ttatacgaga aggctatgtg tatcataaat atgcgttaat aaagtgttaa taaaatatag
      601 actttccgtc tatttacctt ttctgattat tctgcaaaca taagtggtaa ccagaagata
      661 aacagcggga ggtgttattg aaaagatttg gtacacgttc tgcaacaggt aagatggtaa
      721 aactaaaatt acctgtagat gtggaaagtc tattaattga ggcaagtaac agaagcggaa
      781 gaagtcgatc gtttgaggca gtaataagac ttaaagatca tcttcaccgc tatccaaagt
      841 ttaacagggc aggaaatatc tatggtaagt cgctggttaa gtatctgaca atgcgtctgg
      901 atgatgaaac taatcagcta cttattgcag caaaaaatcg tagtggatgg tgtaaaacag
      961 atgaggctgc agacagagtt attgatcatt tgatcaagtt tcctgatttt tataactcgg
     1021 agatattcag ggaggcagat aaagaggaag atataacatt taatacactc tagttttatt
     1081 catttatccg aaattgaggt aacttatgaa tactgtttta agtgttcagg gtgcttctgc
     1141 gcccgtcaaa agaaatcgtt tttttctaaa ttcactcgtc tgaatatgct tcgcctggtt
     1201 cgtgcagtga tcccggttgc tgttctgatg atgttgttcc ctgagttagc tatggctgct
     1261 ggtaaaggcg acctgatggc taaaggtcag gaaacggtta aggcgacctt cggtaagaac
     1321 tccagtatcg ttaaatggat tgttctggct gaggtgctgg tcggtgcggt catgtacatg
     1381 atgaccaaaa acatcaagtt cctggtcggc tttgccatac tctctgtatt tattgcagta
     1441 ggtatgtctg ttgccggatt ctgacaggaa ataaaacgat gtcgggagac gagaataaac
     1501 ttaagaaata tcgtttcccg gaaacactga ccaaccagag ccgctggttt ggcctgccac
     1561 tggatgaact gatccccgca gcaatctgta ttggctgggg tatcataaca tcgaaatatc
     1621 tgttcggtat tggtgcagcg gttctggttt atttcgggat taaaaaactg aaaaaagggc
     1681 ggggcagttc ctggttacgt gacctgattt actggtatat gccaacagcc ctgctgcgcg
     1741 gtatttttca taatgttccc gattcgtgtt tccggcagtg gattaaatag aactgatacc
     1801 aggattgtta tatggaacac ggtgcccgtt taagtaccag tcgtgtaatg gccatcgcct
     1861 ttatatttat gtcagtgctt attgttctca gcctctctgt taacctcatt cagggggtga
     1921 ataactaccg tcttcagaat gagcaacgca ctgctgtgac cccaatggga tttaatgtgc
     1981 cctttgccgt gtcacagaac agtgccgatg cctcttattt gcagcagatg gcgctgtcat
     2041 ttattgccct ccgtctgaat gtctcaccgg aaatcgtcga tgcttcacat caggcgcttc
     2101 tgcaatatat ccgcccgggt gcacagaacc agatgaaagt tattctggct gaagaagcga
     2161 aactcattaa aaaagataac gtgaattctg cttttttcca gaccagtgtc cgtgtctggc
     2221 ctcagtatgg tcgtgtggaa atccgtggaa ttcggaaaac atagattggt aactccgaac
     2281 cttttactga tatcaaacat tacatcctta ttctgaagcg ggaaaacggg gtgacctggc
     2341 tggataattt cggggaaaca gacgatgaga aaaaataata cggcaataat attcggcggc
     2401 ctgttttttt cctgcagcgt gatggccgca aacggtacgc tggcccccgc tgtggtgcca
     2461 atggtgaacg gtggtcaggc cagtattgcc atcagcaata ccagcccgaa tctgtttacc
     2521 gttcccggtg accggattat cgccgtgaac agtctggatg gtgccctgac caataatgag
     2581 cagaccgcct ccggcggtgt ggtggtggcc accgtcaaca aaaagccctt tacgttcatt
     2641 ctggaaacag agcgtggtct gaacctttcc attcaggctg ttccccgtga aggcgcgggg
     2701 cgtaccattc agctggtcag tgacctgcgc ggaaccggag aagaagccgg agcgtgggaa
     2761 acgtccacgc cttacgaatc cctgcttgtg accatcagcc aggctgtccg tggcggaaaa
     2821 ttacctgcag gctggtatca ggttcctgtg acaaaggaaa ccctgcaggc cccggcgggg
     2881 ctgtcttcag tggcagattc cgtatggacg gggaatcacc tgaagatggt ccgctttgtc
     2941 gtggaaaata aaacgctgtc tgccctgaat atccgggaaa gtgatttctg gcagccggga
     3001 acccgtgccg tgatgttcag ccagcctgcc agccagttac tggcaggtgc gcgcatggat
     3061 gtgtatgtca tccgtgacgg ggagggcaac tgatggccag tatcaatacc attgtgaaac
     3121 gcaagcagta cctgtggctg gggattgtgg ttgtcggtgc agcctccgcg attggtgggg
     3181 tactgtatct gtctgatgtg gacatgtccg gtaacggtga ggcggtggct gaacaggagc
     3241 ctgtgccgga tatgacaggt gtggtggata cgacctttga tgacaaggtg cgtcagcatg
     3301 ccaccacaga gatgcaggtg acggcagcgc agatgcagaa gcagtatgag gaaatccgtc
     3361 gtgaactgga tgtcctgaac aaacagcgcg gtgatgacca gcgtcgtatt gaaaagctgg
     3421 gacaggacaa tgccgccctg gcagagcagg taaaagccct gggtgctaat cccgtcacgg
     3481 cgaccggtga gcctgtacct cagatgcctg cctcaccgcc cgggccggaa ggcgaaccac
     3541 agccaggaaa cacccccgta tccttcccgc cgcagggcag cgttgctgtt ccaccgccga
     3601 cggcgtttta tcccgggaat ggtgtcacgc caccaccaca ggtgacgtac cagtctgtgc
     3661 cggtgcctaa ccggatacag cgtaaggtgt ttacccgtaa tgagggaaaa cagggaccat
     3721 cgctgccgta cattccgtca ggaagttttg cgaaagccat gctgattgaa ggggcggatg
     3781 ccaatgcctc agtcaccggt aatgaatcca cggtgccgat gcagttgcgt atcaccggcc
     3841 tggtggaaat gccgaacagc aagacgtatg acgcaacggg atgttttgtg gggctggaag
     3901 cctgggggga tgtgtccagt gaacgtgcca ttgtacgcac ccgcaatatc agttgcctga
     3961 aggacggcaa aactattgat atgccgatta aggggcatgt cagcttccgg ggtaaaaacg
     4021 gtatcaaggg cgaagtggtg atgcgtaacg gcaaaatcct cggctgggca tggggcgcgg
     4081 gatttgttga cggtatcggt cagggaatgg agcgcgcctc ccaaccggct gtcgggctgg
     4141 gtgccacagc cgcttacggg gctggtgatg tcctgaaaat gggtatcggt ggcggcgcat
     4201 cgaaagccgc acagacgctc agtgactact acatcaaacg tgccgaacag tatcacccgg
     4261 tgataccgat tggtgcgggc aacgaagtga ccgtggtgtt ccaggacggc ttccagctga
     4321 aaaccgtgga ggaaatggcg ctggagcagt cacagagtac agcggaagag ggtaacccgg
     4381 aaagccctgt gccgaatcct ccctcggcag aaagccacct gaacggtttt aacaccgacc
     4441 agatgctgaa acagctgggc aatctgaatc cgcagcagtt tatgtccgga gacaagaacc
     4501 cgctgggcgg ggatgccggc aaggagggcg gaaatggcgg gtgatgcact gacattccgt
     4561 gtggcgtttg tcgttcgctg gctgctgtgg gtcgtcaggt ttgtggttat ctggccgctg
     4621 gccacaatgg ccctgatggc tctgtttgtt ttatggaagg acaacaccac gccggggaaa
     4681 ttactggtta aggagattaa cttcgtaaga caaacagcac ctgccggaca gttcccggtc
     4741 agtgaatgct ggttttcttc ctctgattct tcaggcagat cagaaataca ggatatctgc
     4801 cattaccgcg cggctgatgc tgcagattat gtcagggaga ctgaccggtc actgatgcaa
     4861 ctggtcaccg cattatgggc aacgctggca ctgatgtata tgtcgtttgc ggcaggcacc
     4921 gggaaatatc cggtgcggcc cggaaaaatg aaatgtatcc gggtggtaac cgcagatgaa
     4981 catctgaagg aagtttatac ggaagacgct tccctgccgg gaaaaatcag gaaatgccct
     5041 gtttatcttc ctgatgacag aacaaacaga aacaacggag ataaaaatga acatgcgtaa
     5101 tattaatgtt attactgccc gcgctgtgcc ggataaaaac gtatcagatg attttatgca
     5161 tgctgttctc agtaactgca ccacaagaat cgcacttcct gcgccgaaga aattcagttc
     5221 tgaatcattg ccgcacaatt ttaatatggc tgctgtcgga gtgatgaaga agggtatgtc
     5281 gatgagttta ttttcttctc cgggaaatac agtagtctcc ggacaaccct gtgcaggaaa
     5341 aacaaatctg acagtagagt taatttctgc tgttgtggag agtaataaaa aaggagaagg
     5401 gtaatgaaac agatttcttt atttattcct ctgctgggaa ccttattact ttccggctgt
     5461 gcaggaacga actctgattt tgagtgtgat gccaccacgt cagatacctg tatgacgatg
     5521 gagcaggcta atgagaaggc caaaaaactg gagcagtcct cagaagcaaa gccggttgcg
     5581 gcatcactgc cgcgcctggc tgaagggaac ttccggacca cgtcggtgca ggccgttgcg
     5641 gcgacaacgc cgtcccgcag caggcctgcg gtgacagcac atccggagca gaaactgctg
     5701 gcaccacgcc cgctgtttac ggctgcccgg gaagtgaaaa cggttgttcc ggtcagttca
     5761 gttgcgccgg taacacctcc ccgtccgcta agaacgggtg agcagacggc agcattatgg
     5821 atagcgcctt atattgataa tcaggatgtt tatcatcagc catccagtgt gttttttgtt
     5881 attaaaccgt ctgcgtgggg aaaaccacgt attaattaac tggccctgaa gttgtatatc
     5941 agccggcaat gccggcgggc tttctgtatt caaaatctgc ctgtaaaacg agtgccctgt
     6001 gcgtgaaaag ggatgggcgg tagcgtgtca gaacatgtcg ggaggaatat tccgtgagtg
     6061 atgaagccga tgaagcatat tcagtgacag aacaactgac catgacagga ataaaccgga
     6121 tacgccagaa aataaatgtt catggtattc ctgtttatct ctgtgaagca tgcggaaatc
     6181 ctattccgga agcccggcgg aaaatatttc ccggtgtgac gttgtgcgtt gaatgtcagg
     6241 cgtatcagga aaggcagagg aaacactatg cataaatcag aaaatgaaaa aatagatttt
     6301 attctggatg agcatgagca gatattcaga aaacagggtt ctttgtgtat ggcactgggt
     6361 aagggctttt tagttatagc tattctttgt ggtgttgcca cattctgtgt ttctggcctg
     6421 tatctgaaat ccctgtgcct gtccgttgcc tgctgttgtg gggtattcag tcgtattttt
     6481 aattattaca gcgtaccgga ggaatcacgc atggtactca gtcgtcagga attactccgg
     6541 cttatgcgtc tgactgaaga cgtaccagat gtgagacagg aactgcttaa ccgtttgttg
     6601 tccggtaaaa aactgactgg tcgggatgaa caggatatcc ggcgtctctg gcaggaaacg
     6661 cagaggaaac actatgcata aatcagaagc tgaaaaaata gattctattc tggatgaaca
     6721 tcaacagata tatcgcagac ataaccaaat aaataacatc ctggcgtatt gtgcgtgtat
     6781 tgcatggatt ccctgtatta ttgctgcgtt cggcgttgat ggtatatatc tgaaaatcct
     6841 gtttgcggtc cttgcctgct gtggtgcggt atgctttttt attttcttct tcactctcct
     6901 gccggaatca ctcatggtcc tcagccgtca gagcctgctt cagcttatgc gtctgactga
     6961 agacgtaccg gatgcgagac aggaactgct taaccgtttg ttgtccggta aaaaactgac
     7021 tggtcgggat gaacaggata tccggcgtct ctggcaggag aaagtggatg caatgcagga
     7081 gtctgccacc cgtcagcgtg aacaggatac tattcggaag tttactgaag ggaataaatc
     7141 cgaatgaaaa tctgcagtgt tttatgacct cataagttaa ctgaagggat attcagtgat
     7201 taatatacaa gcggagataa taaagaatgc atacctttat ttttcttgta ctgataaaaa
     7261 atattaacgg tggcggaagc tggaatatta cacccatgcc aaatacggac gtatgcgtgc
     7321 aggttcttcg gacaatcgag tcacacggag gcaggggatt tggaaataca ggggaatgta
     7381 ttgaagttaa aacaaataaa cctttgaaca acgtgatgga tacactgaat ggtggtgggc
     7441 aatgagtgct tactgcttca aaggaaaatg aaatgaagtc gatatattta gaatctgttc
     7501 tggcttttat atttgttggc gttatggcta tgtttaagtc ttggtaaggc aaaagagctt
     7561 gcatcagcgt gtagagaaag aaacgaaatg gcagaggtga aacgggtaag agaaaatgag
     7621 cgaaataaga tacgtgaaaa acaacttcag gcattaaatg atgctcattc agtaaaggaa
     7681 cagttaatcc ttgtttcctg ttaaaagcct ccatctctta tttgtgctgt ggtttcagga
     7741 atggcactgc gtgatatatc cacggtcaca gactgatatt ttcctttttt aaacgcacta
     7801 aggaatactc atatgagtaa taacccactt gaggccgtca ctcaggcggt taactccctc
     7861 gtcacagcac tgaaactgcc tgacgaatcc gcaaaggcca atgaagttct gggcgaaatg
     7921 agcttcccgc agttcagccg tctgctgccg taccgtgatt acaaccagga atccggtctg
     7981 ttcatgaatg acaccacgat gggctttatg ctggaagcca ttcccatcaa tggggcgaat
     8041 aagtccattg tggaggcact cgatcacatg ctgcgcacca aactgccgcg cggtattccg
     8101 ttgtgtatcc atctgatgtc cagtcagctg gttggtgaca ggattgagta cgggctgcgt
     8161 gagttctcct ggtcaggtga acaggctgaa cggtttaacg ccattacccg tgcctattat
     8221 atgaaagcgg cagcgacaca gtttcccgct gccggagggg atgaatctgc ccctgaccct
     8281 gcgccattac cgggtattta tctcgtactg ttctccctcg aagaaaaaaa gccgggctga
     8341 cattctggaa atggaaaatc ctggtgaaaa tcatccgtgc gtctttcaca gggcgcaagt
     8401 atcgccacac agacggtgga tgcacaggcc tttatcgata ttgtcgggga gatgattaac
     8461 cataacccgg actccctgta cccgaaaaga cgtcagctgg acccgtattc tgatctgaat
     8521 tatcagtgtg tggaggacag ttttgacctg aatgtccggg ctgattacct gacgctgggc
     8581 ctgcgtgaga acggcaggaa cagcacggcc cgtatcctga atttccatct ggcccgtaac
     8641 ccggaaatcg ccttcctgtg gaatatggcc gacaactaca gcaacctgct gaacccggaa
     8701 ctgtccatct cctgtccgtt catcctgacg ctgactctgg tggtggaaga ccaggtgaaa
     8761 acccacagcg aagccaacct gaagtacatg gacctggaga agaagtcgaa gacctcctat
     8821 gccaaatggt ttccgtccgt ggagaaagag gcgaaggagt ggggggaact gcgtcagcgg
     8881 ctgggctccg gtacagtctt ctgtcgtgtc ctacttcctc aacatcacag ccttctgcaa
     8941 ggacaactaa tgaaacggca gctggaagtg gagcaggaca tcctgaacag cttccgtaaa
     9001 aacggttttg agctgatttc accgcgcttt aaccacatgc gcaatttcct gacctgtctg
     9061 ccctttatgg ccgggaaagg gttgtttaaa cagctgaaag aggccggatg tggttcagcg
     9121 cgcagagagc tttaatgtgg ccaacctgat gccgttagtg gcggataacc ccctgacacc
     9181 ggcaggtctg ctggcaccca cctaccgtaa ccagctggcg tttatcgata tcttctttca
     9241 ggggatgaat aacaccaact acaacatggc ggtctgtggc acctccgggg ccggtaaaac
     9301 cggactgata cagccactta tccgcagcgt gctggactcc gggggctttg ccgtggtgtt
     9361 cgacatgggg gatggctaca agtccctgtg tgagaacatg ggtggcgtat atctggacgg
     9421 tgaaaccctg cgttttaacc cgtttgcgaa catcaccgat atcgaccagt cagcggagcg
     9481 tgtccgtgac cagttgtcag tgatggccag ccccaacggt aacctggatg aagtgcatga
     9541 aggtctgctg ctgcaggcgg tcagggcctc ctggctggcc aaagagaaca gggcacgtat
     9601 tgatgacgtg gtggatttcc tgaaaaacgc cagtgacagc gagcagtatg ccgggtcacc
     9661 gaccatccgc agccgtctgg acgaaatgat tgtgctgctt gaccagtaca ctgccaacgg
     9721 cacttacggc cagtatttta actctgatga gccgtccctg cgggatgacg cgaaaatggt
     9781 ggtgctggag ctgggcggac tggaagaccg tccgtcactg ctggttgcgg tgatgttctc
     9841 cctgattatc tacatcgaga acaggatgta ccgcacgccg cgtactctca agaaactgaa
     9901 cgtcattgat gaaggctggc gtctgctgga cttcaaaaat cgtaaagtcg gtgagtttat
     9961 tcaaaaaggt taccgcacct gtcgccgtcc ataccggtgc ctatatcacc atcacacaga
    10021 acatcgtcga ctttgactct gacaaggcct ccagtgcggc ccgtgcggca tggggtaact
    10081 cctcctacaa aattatcctc aaacagagtg cgaaggagtt cgcgaaatat aaccagttat
    10141 tcccggatca gttccagcca ctgcagcgcg acatgattgg taagtttggt gcggccaaag
    10201 accagtggtt cagttccttc ctgctgcagg tggaaaacca ttcctcctgg caccgcctgt
    10261 ttgtggaccc gttaagccgc gccatgtaca gctctgacgg cccggatttt gagtttgtgc
    10321 agcagaaacg taaagagggg ctgagtattc atgaggcggt gtggcagctg gcgtggaaga
    10381 agtcagggcc ggagatggct tcgctggaag cctggctgga agaacatgag aaatacagga
    10441 gtgttgcatg accacaacgc aaaaaacgac tgatgttaca gccccccgac gcagccactg
    10501 gtggtggacg gtgccgggat gcctggcaat ggtgttactg aacgcagccg tcagttacgg
    10561 cattgtcaga ctgaacgcac cggtgactgc cgccttcaac atgaaacaga ctgtggatgc
    10621 attttttgac agcgccagtc agaaacaact gtcagaagca cagtcaaaag ccctttcagc
    10681 gcgttttaac acggcactgg aagccagcct gcaggcgtgg cagcagaaac accacgcggt
    10741 cattctggtg tcgcctgccg tggtacaggg ggcaccggat atcacccggg aaatccagca
    10801 ggatattgcc cggcgaatga gagcggaacc atgagatgcc gggggctgat tgccctgctg
    10861 atatgggggc agagtgtggc cgccgccgat cttggcacct ggggcgatct gtggccggta
    10921 aaggagccgg acatgctgac ggtgatcatg cagcgcctga cggcactgga gcagtccggt
    10981 gagatgggcc ggaaaatgga tgcctttaag gagcgggtga tccgcaacag tctgcggccc
    11041 cctgccgtgc ccggtatcgg acgcacggag aaatacagca gccggttgtt tgacccgtcc
    11101 gtcagactgg ctgcggatat ccgggataac gaagggcggg tgtttgcccg tcagggtgaa
    11161 gtgatgaatc cgctgcagta tgtgcctttt aaccagacgc tgtatttcat caatggcgat
    11221 gatccggcgc aggtggcctg gatgaaacgc cagacgccgc ccacactgga gagcaaaatt
    11281 atcctcgtgc agggcagtat cccggagatg cagaagtctc tggacagccg tgtttacttt
    11341 gaccagaacg gtgtgctctg ccagcgcctc ggcattgatc aggtcccggc acgggtgagc
    11401 gccgttcccg gcgatcgctt cctgaaggtg gaatttattc cggcagagga gggcagaaaa
    11461 tgaagcgaag cctgtggctg ctgatgttat tccttctcgc cggtcatgtc cctgcggcat
    11521 ctgcggattc tgcctgtgag gggcgttttg taaacccgat cacggatatc tgctggagct
    11581 gtattttccc gctctcgctg ggcagtatca aagtcagtca gggtaaggtc cccgacacgg
    11641 cgaacccgtc gatgcccatt cagatttgtc cggcaccgcc gccgctgttc aggcgtatcg
    11701 ggctggccat tggttactgg gagccgatgg cgctgacgga cgtcacccgg tcaccgggat
    11761 gcatggtgaa cctgggcttc agcctgccgg cttttggtaa aacggcacag ggaacggcga
    11821 aaaaggatga gaagcaggtg aacggggcgt tctatcacgt tcactggtac aaatacccgc
    11881 tgacgtactg gcttaacatc atcacatcgc tgggctgtct ggaaggtggt gacctggata
    11941 tcgcttatct ttctgaaatt gaccccacct ggacggacag cagcctgacc accattctca
    12001 atccggaagc tgtcatcttt gccaatccga tagcacaggg ggcctgcgca gcagatgcca
    12061 ttgccagtgc ctttaatatg cctctcgatg ttctgttctg gtgtgccggt tcgcagggca
    12121 gtatgtaccc gttcaatggc tgggtgagta atgagtccag tccgttgcag tcctccctgc
    12181 tggtcagtga acgcatggcg ttcaaactac accgtcaggg catgattatg gaaaccatag
    12241 ggaaaaataa cgccgtctgt aatgaatatc cgtctccaat cctgcctaaa gaacgctggc
    12301 gttatcagat ggtgaatatg tatccggaca gcggacagtg ccatccgttc gggcgcagtg
    12361 tgatgcgctg ggagacggga aaaaatccgc ccaacacaaa gaaaaacttc ggctacctga
    12421 tgtggcgtaa acgtaactgt gtattcctgt aacttaaaca aaaagaggaa tattttaaat
    12481 ggaaaagaaa ttaagcagta aatacattca tatgtatatg agtatatccc gagtgttcat
    12541 tcgtttgaca ggcaatgtat taaaactgtt tccttttgct atggtgatat gccctgtggt
    12601 gtggttttat ctttgccctg aacatattat ctcagacatt gttaacgact ggcaggatgc
    12661 agatacactg gaaaaaatac tgcttgtcaa actgtatctg aagacggttc ttattctgac
    12721 agtggtaagc gttgttgtct tgtctctttc gtcagataag tttatgtcct gttaagcgca
    12781 ggaaatgagt ctgtcggttc tcataaatat aaccgtgtgt gcatatgtaa aatataaata
    12841 aaggtgcgtt atgagttttt tatcattttt acgaaagata agtaataaag aaaaagaaat
    12901 ttttatcagt gaggagcgtt ttttctccct gctggaaagg gtgagtgctt ttgatgtggt
    12961 cagagaatgg gctgaaaatg gtggagcata ttatcctctg ctggagtgcc gtcggcttac
    13021 agatattgaa gatgtcagga aatcagagga tatactgaac cgactggagt ttattgacag
    13081 aagatttcgg gaatttatca gcattatgtc tgaatataat cgtaatgata aatctaaaaa
    13141 caggtgggag agggaattgt ctgaatatcc accggaaaaa atataccagg tccggagaga
    13201 gggaaaccat ctgtcattaa aaaattacgg tgatgatatt actctgaaca gagataccgc
    13261 cgtggctacg gtgcagatta ctgtcagcag aaaatgcatt actcaggaag aaatggatga
    13321 attatatgca ttgtctgaga gactgaaaga aaagtgtgcc ggtaattaaa ttatatcgct
    13381 atgccgggag atgatatgag ccggaaaata tttgtacttt ttgccctgac cacaggactg
    13441 tttatttctg cccatgcttc ggaaaacgtg aatacgcccg aaaaccgcca gttcctgaag
    13501 cagcaggaaa cactgagtca gcagttacgt gaaaagccgg acacacagtt gcaggcatgg
    13561 gcagagcagc aggtcagggc gaatcccctt cagcattccg ataaccagtt tctcgatgaa
    13621 ctggtccgta aacagcaggc tgctcagaag gataaaccac agcagggcgc cgtgtacttt
    13681 gtgtcgtttt ccattcccga agaggggctg aaacggatgc tgggcgaaac ccggcactac
    13741 ggtattccgg ccacactgcg gggcatggtg aacaatgacc tgaagaccac cgctgaagcc
    13801 gtgttgtccc tggtgaaaga cggcgcaact gatggtgttc agatcgaccc gacgctgttt
    13861 tcacagtacg gcattcgcag cgtaccggcc ctggtggtgt tctgcagcca ggggcacgac
    13921 atcatccggg gaaatctgcg cgtcaggcag gcgctggaga aagtggccgc cgcaggagac
    13981 tgccggcagg tggcccgggc gttgctgaat aaccccggag ataaacaaaa atgactttac
    14041 ctgccgggta ttaccggatt gacccggaca tccgtgcact ggtggccgcg atgaacgttc
    14101 acggatttcg tacatatgcg tcctgtcagg ggcatggttt ccctgttacg aaactgccgc
    14161 cttatattgc ctttgtctgt ccggtaaaaa aggcggctct tcttgagcag cgactgcggc
    14221 aggatgcgga gtccatgatg ccccggttgc tgtggggctg gtccgtcggg gcttctttta
    14281 acagtgacct tcagctctgt ttccggcttc agcctgaagg ccctcatcac tggtatcacc
    14341 ggtactgtcg ccgctcactg cgtgcagact tcaggacgct ggtccgctta ctgaatccgt
    14401 aaatctgttc ccgtaaggag gcattgatgg cgctgacaaa gaagcagcgg gaaaaactgc
    14461 gtatgaaatt cggcggtcgt tgtgcgtact gcggctgtga actgccggaa aaaggctggc
    14521 atgccgacca tgttcaggcg gtgctccgca agtcagagca gtgcatgaag gctgcagaaa
    14581 agcgcatttt caggctgaat atccaccggt gatgttttca gaccggaggc cgactgtccg
    14641 gaaaacctcg tcccggcctg tgcgccgtgc aacctgctga aaacaacata ttcgctggag
    14701 atgttcagaa cgcaggtgtc cctgcaggtt gaacgggggc gcagaagcag cgtgaatttc
    14761 aggaccgcag aacggttcgg gctgattcag gtagtgaaca aaccagtcgt gttctggttt
    14821 gagcaatatc agagggagag tgaggtatga aacgtcttct gccattgctg ccggggctgg
    14881 cagtcagtgt ggcacaggct gacagcaaca gtgattaccg ggcgggttct gattttgccc
    14941 gtcagattca ggggcagggt acaggcagta ttcagggctt taagccacag gagagtatcc
    15001 ccagctataa cgcgaatccg gacgagacaa aatattacgg tggcgtgact gccggcgggg
    15061 atggcggcct gaaaaatgac ggcaccacgg aatgggcgac cggtgaaacc ggaaaaacca
    15121 tcacagagtc cttcatgaac aagccgaaag acattctgtc accggatgca ccgttcattc
    15181 agaccggcag ggatgtggtg aaccgggctg acagcattgt gggaaatacc ggacagcagt
    15241 gcagtgcgca ggagataaac cgcagtgaat tcacaaacta tacctgtgag cgggacacga
    15301 tggtggagga gtactgcaca cggactgcca gtatcaccgg ggactggaaa tacacggatg
    15361 aatatcgtga agtgacgata ccgcactcac agttccggtt cagtatgaac gggctgaaac
    15421 tggtgttcag tgtcacggct ccggtaaccg gtacagtgga aagtgcatca ttgtctgtat
    15481 acgccgcttt tttcttcctg aactcccgtt ataccttcat gaatacaacc tttaatgtcg
    15541 gcctggcaaa cgggcagtcg gatacttacc ccctgtccgg ggcgacaggt ttgcagg
//
