LOCUS       NZ_CP069550            22870 bp    DNA     linear   CON 17-JUN-2023
DEFINITION  Escherichia coli strain FDAARGOS_1253 plasmid unnamed1.
ACCESSION   NZ_CP069550
VERSION     NZ_CP069550.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN16357395
            Assembly: GCF_016889865.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22870)
  AUTHORS   Sokurenko,E., Tallon,L., Sadzewicz,L., Zhao,X., Boylan,J., Ott,S.,
            Bowen,H., Vavikolanu,K., Mehta,A., Aluvathingal,J., Nadendla,S.,
            Yan,Y. and Sichtig,H.
  TITLE     FDA dAtabase for Regulatory Grade micrObial Sequences (FDA-ARGOS):
            Supporting development and validation of Infectious Disease Dx tests
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 22870)
  AUTHORS   Sokurenko,E., Tallon,L., Sadzewicz,L., Zhao,X., Boylan,J., Ott,S.,
            Bowen,H., Vavikolanu,K., Mehta,A., Aluvathingal,J., Nadendla,S.,
            Yan,Y. and Sichtig,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2021) Institute for Genome Sciences, University of
            Maryland School of Medicine, BioPark II, 801 W. Baltimore St., Suite
            619, Baltimore, MD 21201, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: SMRT v. 8.0.0, HGAP v. 4, SPAdes v. 3.14.1
            Genome Coverage                   :: 1090.86x
            Sequencing Technology             :: Pacbio; Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 06/17/2023 05:01:47
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,554
            CDSs (total)                      :: 4,441
            Genes (coding)                    :: 4,315
            CDSs (with protein)               :: 4,315
            Genes (RNA)                       :: 113
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 49 of 126
            Pseudo Genes (incomplete)         :: 81 of 126
            Pseudo Genes (internal stop)      :: 27 of 126
            Pseudo Genes (multiple problems)  :: 27 of 126
            Pseudo Genes (short protein)      :: 3 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP069550.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..22870
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="FDAARGOS_1253"
                     /host="Homo sapiens"
                     /culture_collection="FDA:FDAARGOS_1253"
                     /db_xref="taxon:562"
                     /plasmid="unnamed1"
                     /geo_loc_name="USA: WA"
                     /collected_by="ARMADA"
     gene            <1..804
                     /locus_tag="I6J83_RS22200"
                     /old_locus_tag="I6J83_22200"
     CDS             <1..804
                     /locus_tag="I6J83_RS22200"
                     /old_locus_tag="I6J83_22200"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001617890.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006304 - DNA modification [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="WP_251302542.1"
                     /translation="PDNPEKSLAIQSEIVRILDKFTALTAELTAELTAELNMRKKQYNY
                     YRDQLLTFKEGEVEWKALGEIGEFIRGKRFTKADYVEDGGISVIHYGEIYTRYGVYTTH
                     SLSQVRADMAASLRYAKHGDVVITDVGETVEDVGKAVAWLGDDDIAIHDHCYAFRHSLN
                     PKFISYYMQTDSFISEKAKYVARTKVNTLLINGFSKIMIPVPYPKDHEKSLKEQARIVE
                     ILDKFDTLTNSITEGLPREIELRQKQYEYYRDLLFSFPKPETVSN"
                     /name="restriction endonuclease subunit S"
     gene            926..4042
                     /locus_tag="I6J83_RS22205"
                     /old_locus_tag="I6J83_22205"
     CDS             926..4042
                     /locus_tag="I6J83_RS22205"
                     /old_locus_tag="I6J83_22205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001764166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HsdR family type I site-specific
                     deoxyribonuclease"
                     /protein_id="WP_001553854.1"
                     /translation="MTHQTHTIAESNNFIVLDKYIKAEPTGDSYQSESDLERELIQDLR
                     NQGYEFISVKSQSAMLANVREQLQNLNGVVFNDSEWRRFTEQYLDNPSDGILDKTRKIH
                     IDYICDFIFDDERLENIYLIDKKNLMRNKVQIIQQFEQTGSHANRYDVTILVNGLPLVQ
                     IELKKRGVAIREAFNQIHRYSKESFNSENSLFKYLQLFVISNGTDTRYFANTTKRDKNS
                     FDFTMNWAKSDNTLIKDLKDFTATFFQKHTLLNVLVNYSVFDSSQTLLVMRPYQIAATE
                     RILWKINSSYKAKNWSTPESGGFIWHTTGSGKTLTSFKAARLATELDFIDKVFFVVDRK
                     DLDYQTMKEYQRFSPDSVNGSENTAGLKRNLDKDDNKIIVTTIQKLNNLMKAESDLPVY
                     NQQVVFIFDECHRSQFGEAQKNLKKKFKRYYQFGFTGTPIFPENALGSETTASVFGREL
                     HSYVITDAIRDEKVLKFKVDYNDVRPQFKSLETETDEKKLSAAENQQAFLHPMRIQEIT
                     QYILNNFRQKTHRTFPGSKGFNAMLAVSSVDAAKAYYATFKRLQEEAANKSATYKPLRI
                     ATIFSFAANEEQNAIGEISDETFDTSAMDSSAKEFLDAAIREYNSHFKTNFSTDSNGFQ
                     NYYRDLAQRVKNQDIDLLIVVGMFLTGFDAPTLNTLFVDKNLRFHGLMQAFSRTNRIYD
                     ATKTFGNIVTFRDLERSTIDAITLFGDKNTKNVVLEKSYAEYMEGFTDAATGEAKRGFM
                     AVVAELEQRFPDPASIESEKEKKDFVKLFGEYLRTENILQNYDEFATLKALQQIDLSDP
                     VAVEKFKAEHYVDDEKLAELQTIRLPADRKIQDYRSAYNDIRDWQRREKEAEKKEKSTT
                     DWDDVVFEVDLLKSQEINLDYILGLIFEHNRQNKGKGEMIEEVKRLIRSSLGNRAKEGL
                     VVDFIQQTNLDDLPDKASIIDAFFTFAQHEQLREAEALIKEENLNEEAAKRYIRTSLKR
                     EYATENGTELNETLPKLSPLNPQYKTKKQAVFQKIVSFIEKFKGVGGKI"
                     /name="HsdR family type I site-specific deoxyribonuclease"
     gene            4256..4953
                     /locus_tag="I6J83_RS22210"
                     /old_locus_tag="I6J83_22210"
     CDS             join(4256..4505,4505..4953)
                     /locus_tag="I6J83_RS22210"
                     /old_locus_tag="I6J83_22210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743396.1"
                     /ribosomal_slippage=""
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1-like element IS1A family transposase"
                     /protein_id="WP_103215986.1"
                     /translation="MASVSISCPSCSATDGVVRNGKSTAGHQRYLCSHCRKTWQLQFTY
                     TASQPGTHQKIIDMAMNGVGCRATARIMGVGLNTILRHFKKLRPQSVTSRIQPGSDVIV
                     CAEMDEQWGYVGAKSRQRWLFYAYDRLRKTVVAHVFGERTMATLGRLMSLLSPFDVVIW
                     MTDGWPLYESRLKGKLHVISKRYTQRIERHNLNLRQHLARLGRKSLSFSKSVELHDKVI
                     GHYLNIKHYQ"
                     /name="IS1-like element IS1A family transposase"
     gene            complement(5090..6022)
                     /locus_tag="I6J83_RS22215"
                     /old_locus_tag="I6J83_22215"
     CDS             complement(5090..6022)
                     /locus_tag="I6J83_RS22215"
                     /old_locus_tag="I6J83_22215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001710756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-4TM family putative pore-forming effector"
                     /protein_id="WP_000991832.1"
                     /translation="MNAIYTNQSTRENMDLLYAQARVYDRVKNWNRLNFLFSIIVPLLL
                     SFVTVYNRSREFVDSELLSSLLGLYGLLVLTFNIVISGHISALRRKAASIQEMYDCRVL
                     GIRRNELKVEEIPRDDIIREAAYFRDSPEKARKRFGEEGWYVSKVYDAPQAVMALLCHG
                     KNLGWDKSLREVLHVFYLSAFIVSPVAMLVYGIVMKSGLNEILFYVVFTLPVIRYFLLQ
                     FLDNRSSMKRSEKLKKYVEKELSGIRVSGRAEEEQLAYTLRNIQDEMFTYRASCPPVPN
                     GIQLIMKPKNERIYVDYFETNLKELHLQK"
                     /name="S-4TM family putative pore-forming effector"
     gene            complement(6026..7021)
                     /locus_tag="I6J83_RS22220"
                     /old_locus_tag="I6J83_22220"
     CDS             complement(6026..7021)
                     /locus_tag="I6J83_RS22220"
                     /old_locus_tag="I6J83_22220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013578371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000246636.1"
                     /translation="MATTVIAAFNEFMKDTVNLKKADTDDARASRDWLIGKMNDFEKDD
                     KFPVSFPAIHIAFGSFARRTKIRPLDDIDLMFGLTGQGATYTILSDRITVTSSGEGSRL
                     HSYRHSGADTVCSVRILNAFKNRLQDIAQYAQADIRRNQEAVTLKLVSKDWNFDIVPCF
                     ITSEDAFGRTYYLIPDGNGHWKFTDPRKDRDRVTTINVQNNGNVLNVIRAVKYWQRRPT
                     MPSMSSYLLETLILDYYAGRTSCSSFVDMELEALFRHLGQSVRYSVNDPKGIQGDINSL
                     SAEARKAISDRCYLDAQKVSEARWFENNKEYEKSINKWRDVFGPFFPVYG"
                     /name="hypothetical protein"
     gene            7729..7947
                     /gene="ccdA"
                     /locus_tag="I6J83_RS22225"
                     /old_locus_tag="I6J83_22225"
     CDS             7729..7947
                     /gene="ccdA"
                     /locus_tag="I6J83_RS22225"
                     /old_locus_tag="I6J83_22225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin CcdA"
                     /protein_id="WP_000813634.1"
                     /translation="MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWK
                     AENQEGMAEVARFIEMNGSFADENRDW"
                     /name="ccdA"
     gene            7949..8254
                     /gene="ccdB"
                     /locus_tag="I6J83_RS22230"
                     /old_locus_tag="I6J83_22230"
     CDS             7949..8254
                     /gene="ccdB"
                     /locus_tag="I6J83_RS22230"
                     /old_locus_tag="I6J83_22230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin CcdB"
                     /protein_id="WP_001159868.1"
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
                     /name="ccdB"
     gene            8255..9061
                     /locus_tag="I6J83_RS22235"
                     /old_locus_tag="I6J83_22235"
     CDS             8255..9061
                     /locus_tag="I6J83_RS22235"
                     /old_locus_tag="I6J83_22235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052636.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0015074 - DNA integration [Evidence IEA];
                     GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="WP_000016982.1"
                     /translation="MSGSVIHSQSAVMVPAVYSAGQPASLPVAIDYPAALALRQMSMVH
                     DELPKYLLAPEVSALLHYVPDLHRKMLLATLWNTGARINEALALTRGDFSLAPPYPFVQ
                     LATLKQRTEKAARTAGRMPAGQQTHRLVPLSDSWYVSQLQTMVATLKIPMERRNRRTGR
                     TEKARIWEVTDRTVRTWIGEAVAAAAADGVTFSVPVTPHTFRHSYAMHMLYAGIPLKVL
                     QSLMGHKSISSTEVYTKVFALDVAARHRVQFAMPESDAVAMLKQLS"
                     /name="site-specific integrase"
     gene            9835..10590
                     /gene="repE"
                     /locus_tag="I6J83_RS22240"
                     /old_locus_tag="I6J83_22240"
     CDS             9835..10590
                     /gene="repE"
                     /locus_tag="I6J83_RS22240"
                     /old_locus_tag="I6J83_22240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000852146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein RepE"
                     /protein_id="WP_000852146.1"
                     /translation="MAETAVINHKKRKNSPRIVQSNDLTEAAYSLSRDQKRMLYLFVDQ
                     IRKSDGTLQEHDGICEIHVAKYAEIFGLTSAEASKDIRQALKSFAGKEVVFYRPEEDAG
                     DEKGYESFPWFIKRAHSPSRGLYSVHINPYLIPFFIGLQNRFTQFRLSETKEITNPYAM
                     RLYESLCQYRKPDGSGIVSLKIDWIIERYQLPQSYQRMPDFRRRFLQVCVNEINSRTPM
                     RLSYIEKKKGRQTTHIVFSFRDITSMTTG"
                     /name="repE"
     gene            11178..12344
                     /gene="sopA"
                     /locus_tag="I6J83_RS22245"
                     /old_locus_tag="I6J83_22245"
     CDS             11178..12344
                     /gene="sopA"
                     /locus_tag="I6J83_RS22245"
                     /old_locus_tag="I6J83_22245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid-partitioning protein SopA"
                     /protein_id="WP_000772446.1"
                     /translation="MKLMETLNQCINAGHEMTKAIAIAQFNDDSPEARKITRRWRIGEA
                     ADLVGVSSQAIRDAEKAGRLPHPDMEIRGRVEQRVGYTIEQINHMRDVFGTRLRRAEDV
                     FPPVIGVAAHKGGVYKTSVSVHLAQDLALKGLRVLLVEGNDPQGTASMYHGWVPDLHIH
                     AEDTLLPFYLGEKDDVTYAIKPTCWPGLDIIPSCLALHRIETELMGKFDEGKLPTDPHL
                     MLRLAIETVAHDYDVIVIDSAPNLGIGTINVVCAADVLIVPTPAELFDYTSALQFFDML
                     RDLLKNVDLKGFEPDVRILLTKYSNSNGSQSPWMEEQIRDAWGSMVLKNVVRETDEVGK
                     GQIRMRTVFEQAIDQRSSTGAWRNALSIWEPVCNEIFDRLIKPRWEIR"
                     /name="sopA"
     gene            12344..13315
                     /locus_tag="I6J83_RS22250"
                     /old_locus_tag="I6J83_22250"
     CDS             12344..13315
                     /locus_tag="I6J83_RS22250"
                     /old_locus_tag="I6J83_22250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family plasmid partition protein"
                     /protein_id="WP_000817036.1"
                     /translation="MKRAPVIPKHTLNTQPVGDTSLSTPAAPMVDSLIARVGVMARGNA
                     ITLPVCGRDVKFTLEVLRGDSVEKTSRVWSGNERDQELLTEDSLDDLIPSFLLTGQQTP
                     AFGRRVSGVIEIADGSRRRKAAALTESDYRVLVGELDDEQMAALSRLGNDYRPTSAYER
                     GQRYASRLQNEFAGNISALADAENISRKIITRCINTAKLPKSVVALFSHPGELSARSGD
                     ALQKAFTDKEELLKQQASNLHEQKKAGVIFEAEEVITLLTSVLKTSSASRTSLSSRHQF
                     APGATVLYKGDKMVLNLDRSRVPTECIEKIEAILKELEKPAP"
                     /name="ParB/RepB/Spo0J family plasmid partition protein"
     gene            14182..15084
                     /locus_tag="I6J83_RS22255"
                     /old_locus_tag="I6J83_22255"
     CDS             14182..15084
                     /locus_tag="I6J83_RS22255"
                     /old_locus_tag="I6J83_22255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1281 domain-containing protein"
                     /protein_id="WP_001348615.1"
                     /translation="MHFSGEPAQIAEIKRLASGAVTPLYRRATNEGIQLFLAGSAGLLQ
                     TTEDVQFEPCPGLTDAGRGVVSPENIAFTRWLTHLQNGVLLDEQNCLMLHELWLQSGTG
                     QRRWEGLPDEVRETITVHFTAKRGDWCGFWSNEDVSVWWNRLCDNVLPEKTMPFDLLTV
                     LPTRLDVEVNGFNGGVLNGVPSAYHWYTEQYGVKWPVGYEVNISRQGENFIQVDFDTPW
                     CQPESNVVAELSRRFGCTLEHWYAEQGCNFCGWQRYERGELVDVLWGELEWSSPTDDDE
                     LPEVTAPEWIVDKVAHYGG"
                     /name="DUF1281 domain-containing protein"
     gene            15088..15393
                     /locus_tag="I6J83_RS22260"
                     /old_locus_tag="I6J83_22260"
     CDS             15088..15393
                     /locus_tag="I6J83_RS22260"
                     /old_locus_tag="I6J83_22260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_251302543.1"
                     /translation="MTETGGQPPVSFPTKDVAVLLFLLRRLTRRGRNAACGQCPASGGH
                     GTGAFTCSRLASVTVYASLSPFSDERPSSRFRGISSPSKRRRLRYSTVGLTRYRTR"
                     /name="hypothetical protein"
     gene            15470..16153
                     /locus_tag="I6J83_RS22265"
                     /old_locus_tag="I6J83_22265"
                     /pseudo=""
     CDS             15470..16153
                     /locus_tag="I6J83_RS22265"
                     /old_locus_tag="I6J83_22265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858331.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methylase"
                     /name="DNA methylase"
     gene            16154..16375
                     /locus_tag="I6J83_RS22270"
                     /old_locus_tag="I6J83_22270"
     CDS             16154..16375
                     /locus_tag="I6J83_RS22270"
                     /old_locus_tag="I6J83_22270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001104873.1"
                     /translation="MNYAGHEKLRADVAEVANTMCDLRARLNDMEHRCRFDSDVLVERL
                     TRQTLYRANRLFMEAYTEILELDSCFKD"
                     /name="hypothetical protein"
     gene            16305..16823
                     /locus_tag="I6J83_RS22275"
                     /old_locus_tag="I6J83_22275"
     CDS             16305..16823
                     /locus_tag="I6J83_RS22275"
                     /old_locus_tag="I6J83_22275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 family protein"
                     /protein_id="WP_251302544.1"
                     /translation="MAPIACSWRRIPKFWNWIPASKIEKETRMYGTCETLCRELAAKYP
                     GDTPLMLVIWSPEEIQALADGMDISLTDHEIRTVLARLEDIPEDQRTESGISSAAVMEI
                     ISNVSENRQVTVPAELLASLIQTAEQALWKREWAARDNGLAVPECVTRRQAVVNQARTL
                     LKNNTHENN"
                     /name="DUF1380 family protein"
     gene            16874..17651
                     /locus_tag="I6J83_RS22280"
                     /old_locus_tag="I6J83_22280"
                     /pseudo=""
     CDS             16874..17651
                     /locus_tag="I6J83_RS22280"
                     /old_locus_tag="I6J83_22280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052647.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /name="hypothetical protein"
     gene            18069..>18486
                     /locus_tag="I6J83_RS22285"
                     /old_locus_tag="I6J83_22285"
                     /pseudo=""
     CDS             18069..>18486
                     /locus_tag="I6J83_RS22285"
                     /old_locus_tag="I6J83_22285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001198918.1"
                     /note="frameshifted; incomplete; partial in the middle of a
                     contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /name="antirestriction protein"
     gene            18543..18970
                     /locus_tag="I6J83_RS22815"
                     /pseudo=""
     CDS             18543..18970
                     /locus_tag="I6J83_RS22815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052650.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /name="hypothetical protein"
     gene            18967..19158
                     /locus_tag="I6J83_RS22300"
                     /old_locus_tag="I6J83_22300"
     CDS             18967..19158
                     /locus_tag="I6J83_RS22300"
                     /old_locus_tag="I6J83_22300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001027516.1"
                     /translation="MNISTETREILRNYRAVINARRREMGQKPLTTAQIVDEICDFVAN
                     QQAVFLGGHYILQGSRNR"
                     /name="hypothetical protein"
     gene            20207..20425
                     /locus_tag="I6J83_RS22305"
                     /old_locus_tag="I6J83_22305"
     CDS             20207..20425
                     /locus_tag="I6J83_RS22305"
                     /old_locus_tag="I6J83_22305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009310031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_029976502.1"
                     /translation="MVSPSAAAVKPRRHPRFRREDIPAPEIDPVLKAFGRHIARSFHRG
                     RGVHIPAMKNTAFGQVLRTLELKRAFN"
                     /name="hypothetical protein"
     gene            20477..21840
                     /locus_tag="I6J83_RS22315"
                     /old_locus_tag="I6J83_22315"
                     /pseudo=""
     CDS             20477..21840
                     /locus_tag="I6J83_RS22315"
                     /old_locus_tag="I6J83_22315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052653.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3560 domain-containing protein"
                     /name="DUF3560 domain-containing protein"
     gene            21887..22451
                     /locus_tag="I6J83_RS22320"
                     /old_locus_tag="I6J83_22320"
                     /pseudo=""
     CDS             21887..22451
                     /locus_tag="I6J83_RS22320"
                     /old_locus_tag="I6J83_22320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052654.1"
                     /GO_function="GO:0008168 - methyltransferase activity
                     [Evidence IEA]; GO:1904047 - S-adenosyl-L-methionine
                     binding [Evidence IEA]"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /name="class I SAM-dependent methyltransferase"
     gene            complement(22535..>22870)
                     /locus_tag="I6J83_RS22325"
                     /old_locus_tag="I6J83_22325"
                     /pseudo=""
     CDS             complement(22535..>22870)
                     /locus_tag="I6J83_RS22325"
                     /old_locus_tag="I6J83_22325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052655.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /name="hypothetical protein"
     Typing          9694..10081
                     /name="IncFIA"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="88.235"
                     /coverage="100.0"
                     /evalue="1.27e-127"
ORIGIN
        1 ccggataatc cggaaaagtc ccttgccatc cagtctgaaa tcgttcggat tctggataag
       61 tttactgcac ttaccgctga gcttaccgct gagcttaccg ctgagcttaa catgcgtaaa
      121 aaacagtaca actactatcg cgaccaattg ctgactttta aagaaggtga ggttgagtgg
      181 aaagctttgg gggaaattgg tgagtttatt cgtggaaaac gttttactaa agcagattat
      241 gtagaagatg gtggtatcag tgtaatccat tatggtgaga tatatacacg atatggtgtt
      301 tacacaactc attcgttatc acaggttaga gctgatatgg cagcgtcatt acgctatgcc
      361 aaacatggag atgtcgtgat aactgacgtg ggggagactg tagaagacgt tggtaaagca
      421 gtggcatggc ttggggatga cgatatcgct attcatgatc actgttatgc ttttcgacat
      481 tctttgaatc caaaatttat ttcctattac atgcaaaccg attcgtttat ttctgaaaaa
      541 gcaaagtatg tagccagaac aaaagttaat acactactca ttaatggttt ttcaaaaatt
      601 atgattccgg ttccatatcc taaagatcac gaaaaatcac ttaaggaaca agctcgcatt
      661 gtcgaaatac tcgacaaatt cgacacactg actaactcca tcaccgaagg tcttccgcgt
      721 gaaatcgagt tgcgccagaa acaatacgaa tactaccgtg atttactgtt cagtttccca
      781 aaacctgaaa ctgtcagtaa ttaattgacc gttgctaccg accgggccac cttaacatcc
      841 ggtctgtata tagactattt tttacgtgcc ggaagtcacc cttaactccc ttccggccct
      901 tgccagacgg cacaaaggat gcgctatgac tcatcagaca cacaccattg ctgaatccaa
      961 taactttatc gtccttgata agtacatcaa agctgagcca acaggcgaca gctaccagag
     1021 cgaatcggac ctggaacgag aactgattca ggacctgcgg aatcagggct atgaatttat
     1081 atccgtaaaa tcacagtcgg cgatgctggc caatgttcgg gaacagcttc agaacctcaa
     1141 tggtgtggtg tttaatgaca gcgagtggcg gcgtttcacg gagcagtatc tggataaccc
     1201 gagcgatggc attctggata agacccgtaa aatccatatc gactatattt gcgactttat
     1261 ttttgatgac gagcgtcttg agaacatcta tttgatagat aaaaagaatc tcatgcgcaa
     1321 taaggtgcag attatccagc agtttgaaca gacgggttct catgctaacc gttatgacgt
     1381 cacgatcctg gttaatggtt taccgctggt gcaaatcgaa ctaaaaaaac gcggggtggc
     1441 gattcgtgag gctttcaacc agatacatcg ttacagtaaa gagagtttta acagcgaaaa
     1501 ttccctgttt aagtatctgc aactgtttgt catttctaac ggcaccgata cccgttattt
     1561 tgccaacacg acaaagcgcg ataaaaacag ttttgacttc accatgaatt gggcaaaatc
     1621 agacaacacg ctgattaaag acctcaaaga ctttaccgct acctttttcc agaaacatac
     1681 tctgctgaat gttctggtga actacagcgt ttttgacagt agccagacgc tactggtgat
     1741 gcgaccgtac cagattgccg ccactgagcg tatcttatgg aaaatcaaca gctcctataa
     1801 agcgaaaaac tggtctaccc ccgaaagtgg tggctttatc tggcacacta ccggttctgg
     1861 taaaaccctg accagcttta aagccgcgcg tctggcaaca gaactggact ttattgataa
     1921 agtcttcttt gtggtcgaca ggaaagacct cgattaccag accatgaagg aatatcagcg
     1981 tttttcgcca gacagtgtca atggatcgga aaataccgca ggtcttaaac gaaatctgga
     2041 taaggatgat aacaaaatta tcgtcaccac tattcagaaa ctcaataacc tgatgaaagc
     2101 agaaagcgac ctgcctgtat acaatcagca agtggtgttt atatttgatg aatgccaccg
     2161 cagccagttt ggagaagcgc agaaaaacct gaagaagaaa ttcaaacgct attatcagtt
     2221 tggttttacc ggtaccccta ttttcccgga aaacgcctta ggctcagaaa caaccgccag
     2281 cgtatttggt cgtgaattgc attcatatgt tattaccgat gcgattcgag atgaaaaagt
     2341 gcttaaattc aaggtggact acaacgatgt gcggccacag tttaagtctt tagagacaga
     2401 aaccgacgag aaaaaactga gtgcggctga aaatcagcag gcgtttcttc atcccatgcg
     2461 tatacaggaa attacgcaat atattctgaa taatttccgc cagaaaactc accgtacctt
     2521 ccctggctca aaaggtttta atgctatgtt ggcagtgagc agcgtggatg ccgcgaaagc
     2581 ctattatgcg acgtttaaac ggttacaaga agaagccgct aataaatcgg ctacctataa
     2641 accgctgcgt attgcgacaa tcttctcctt tgccgccaat gaagaacaaa atgccattgg
     2701 tgagatttcc gatgaaactt ttgataccag cgcaatggac agcagtgcta aagagtttct
     2761 ggacgctgca attcgtgaat ataacagcca ttttaaaact aactttagca ccgacagtaa
     2821 cggttttcag aactactatc gtgatttagc ccagcgggtt aaaaatcagg atatcgatct
     2881 gctaattgtc gtggggatgt tcttaaccgg cttcgatgct ccaacattga acacgctatt
     2941 cgtcgataaa aacttacgtt ttcacggcct gatgcaggca ttttcccgca ccaaccgcat
     3001 ttatgacgcc actaaaacct tcggtaatat cgtcactttc cgggatctgg aacgctcaac
     3061 tattgatgcc ataacgctgt ttggtgacaa aaacaccaaa aatgtagtgt tagaaaagag
     3121 ttatgcagag tatatggaag gctttactga tgctgctact ggtgaagcta agcgcggctt
     3181 tatggcagta gttgcagaac tggaacaacg gttccctgac cctgccagta ttgaaagtga
     3241 aaaagagaag aaagacttcg taaaactgtt tggtgaatac ctgcgtaccg agaacatcct
     3301 gcaaaactat gatgaatttg ccacgctgaa agccctgcaa caaatcgatc ttagcgatcc
     3361 tgttgcagta gaaaaattca aagcagaaca ttatgtggat gatgaaaagc tcgctgaatt
     3421 gcaaacaatt cgtctccctg ctgatcgcaa gattcaggat tatcgttctg cctataacga
     3481 tattcgcgac tggcagcgcc gtgagaaaga agctgagaaa aaagagaaat caaccactga
     3541 ctgggatgac gtggtttttg aggtcgattt gctgaagtct caggaaataa acctggatta
     3601 tatccttgga ctgattttcg aacataacag acaaaataaa ggcaagggcg aaatgatcga
     3661 agaggtcaaa cgcttaattc gttcaagcct ggggaaccgg gcgaaagagg gcctggtggt
     3721 cgattttatt cagcaaacga acctggatga tttaccagac aaagccagca tcattgacgc
     3781 gttctttacc tttgctcaac acgaacagct acgtgaagca gaagcgttga ttaaagaaga
     3841 aaatctcaat gaagaggcgg cgaaacgcta tattcgcacg tctttaaaac gcgaatacgc
     3901 caccgaaaat ggcacagaat taaacgagac attaccaaaa cttagtccgt taaacccgca
     3961 atataaaacg aaaaaacagg cagttttcca gaaaatcgtc tcgtttattg agaagtttaa
     4021 aggcgtaggc ggaaaaatat agcccaattc gtgtttttct tgcgggttct taattaaacc
     4081 cgcaagagac cgtggggttc caaatggcta atatactccc cttacccatg agcgacgatg
     4141 ccgccaaaag tgatagagaa cagccagaaa tagatcgcgg ccataatgat tttgaatgcc
     4201 ggtgatgctg ccaacttact gatttagtgt atgatggtgt ttttgaggtg ctcctgtggc
     4261 ttctgtttct atcagctgtc cctcctgttc agctactgac ggggtggtgc gtaacggcaa
     4321 aagcactgcc ggacatcagc gctatctctg ctctcactgc cgtaaaacat ggcaactgca
     4381 gttcacttac accgcttctc aacccggtac gcaccagaaa atcattgata tggccatgaa
     4441 tggcgttgga tgccgggcaa ccgcccgcat tatgggcgtt ggcctcaaca cgattttacg
     4501 tcacttaaaa aactcaggcc gcagtcggta acctcgcgca tacagccggg cagtgacgtc
     4561 atcgtctgcg cggaaatgga cgaacagtgg ggatacgtcg gggctaaatc gcgccagcgc
     4621 tggctgtttt acgcgtatga caggctccgg aagacggttg ttgcgcacgt attcggtgaa
     4681 cgcactatgg cgacgctggg gcgtcttatg agcctgctgt caccctttga cgtggtgata
     4741 tggatgacgg atggctggcc gctgtatgaa tcccgcctga agggaaagct gcacgtaatc
     4801 agcaagcgtt acactcagcg aattgagcgg cataacctga atctgaggca gcacctggca
     4861 cggctgggac ggaagtcgct gtcgttctca aaatcggtgg agctgcatga caaagtcatc
     4921 gggcattatc tgaacataaa acactatcag taagttggag tcattacctg tgttactaac
     4981 cgtctgaggc gaaggtgact atgggtttac gctgacactc aggcaaccaa gaattgttta
     5041 tcggatgtgc tccggcatac gtaaatccta caccggagct aaggagggat tacttctgaa
     5101 ggtgcagctc cttcagattt gtttcaaagt aatcaacata aattcgttca tttttgggct
     5161 tcattatcag ctgaataccg ttgggtacgg gcggacatga ggcccggtag gtaaacatct
     5221 catcttggat attccggagc gtataagcca gttgctcttc ttctgccctt ccggacaccc
     5281 ttatgccgga taattccttc tcaacgtact ttttcagctt ctcgcttctt ttcatactgc
     5341 tgcgattatc cagaaactga agcaagaaat agcggatgac cggcagtgtg aacaccacat
     5401 agaagaggat ctcattcagc ccggatttca tgacgatgcc gtaaacaagc atcgcgacag
     5461 gcgagacaat aaaagcggaa agataaaata cgtgcagaac ctccctgagc gatttgtccc
     5521 agccgagatt ttttccgtgg caaaggagcg ccattacggc ctgcggcgcg tcatacacct
     5581 tactgacgta ccacccctct tcgccaaatc gctttcgtgc tttttccggg ctgtctctaa
     5641 aatatgcagc ttcccggatg atgtcatccc gtgggatctc ctccaccttc agttcattac
     5701 gccggatgcc aagtacccgg cagtcgtaca tttcctggat tgaggctgcc ttcctgcgca
     5761 gagccgagat gtgtccactg ataacaatgt taaaggtaag caccaggagt ccatataacc
     5821 ccaacagcga agacaacaac tctgaatcga caaattcccg gctccggttg tatacggtaa
     5881 cgaaagaaag caggagcggt acgatgatgc tgaagagaaa attcagtctg ttccagtttt
     5941 ttaccctgtc gtagacacgg gcctgcgcat acagtaaatc catgttttct ctggtcgact
     6001 ggtttgtgta aatggcattc atgattcacc cgtaaacagg aaagaatggc ccgaacacat
     6061 cgcgccattt attgatggat ttttcatact ctttattatt ttcaaaccat ctcgcctccg
     6121 ataccttttg cgcatcgaga taacatctgt ccgatatcgc cttacgtgcc tcggctgaaa
     6181 gtgagttgat atcaccctgt atgcctttgg gatcgttgac agagtaccgc acggattgac
     6241 caaggtggcg gaaaagcgct tccagctcca tgtcaacaaa cgatgagcag gatgtcctgc
     6301 cagcgtaata gtccagaatc agcgtttcca gcaggtagga actcatggat ggcatcgtcg
     6361 gtcgccgctg ccagtatttg actgctctga tgacattgag cacattgccg ttattctgca
     6421 cattgatagt agtgactctg tccctgtctt tacgggggtc ggtaaacttc cagtggccgt
     6481 tgccatcggg gatcaggtag taagtccgtc caaaggcatc ttcgctggta ataaagcagg
     6541 ggacgatgtc aaaattccag tccttgctga ccagtttcag cgtgacagct tcctggttac
     6601 gcctgatatc agcctgggcg tactgggcaa tgtcctgcag gcggttttta aaggcattca
     6661 gaattctgac ggagcagacg gtgtcagctc cggaatggcg gtaactgtgc agacgtgacc
     6721 cctctccgga ggaggttaca gttatccggt cactcaggat ggtataggtg gcaccttgcc
     6781 cggttaagcc aaacatcaga tcgatatcat caagcggacg gattttggtt cttctggcaa
     6841 aggagccaaa ggcaatatgg atcgctggaa aactcaccgg aaatttatcg tccttctcaa
     6901 aatcattcat cttaccgata agccagtcgc gacttgcacg cgcgtcatcg gtatctgcct
     6961 ttttgagatt cacggtatct ttcataaatt cgttaaaggc agctattact gtggtcgcca
     7021 tgttcagtcc tttaattatt tattgcaaaa ggcgcggggc tctgtcgtac atctgaacat
     7081 tccagtgccc gggaagagtc ggtacagttt attattagtg ccgcaagttc actaccgtct
     7141 cgctctctgc aagggcatat aatttacatg ggaaatatga gcctcgttct ttcattgata
     7201 attatgcaaa gtatttcagt tattaatgcc agataagcaa taatgcaatt aagttcaaaa
     7261 tatatcatta gtattgcatc atcagcacaa atccttcttc ccctctccag agatccgaaa
     7321 aaagtaagtt acggatcttc ctctccctcc gcacagcgtt acatcccgtc agcacagcat
     7381 gtagtgcctc atacagttgc caatggcact atatgttgtg ttgtatctct ggactgtgat
     7441 gcgccgcgca ggggcggaaa acagcgatat gatgattttc tcagcgttgt acacttccgg
     7501 aaagtcgttt attcaaataa agtcggatcc atacgaaacg ggaatgcggt aattacgctt
     7561 tgtttttata agtcagattt taatttttat tggttaacat aacgaaaggt aaaatacata
     7621 aggcttacta aaagccagat aacagtatgc gtatttgcgc gctgattttt gcggcataag
     7681 aatatatact gatatgtata cccgaagtat gtcaaaaaga ggtgtgctat gaagcagcgt
     7741 attacagtga cagttgacag cgacagctat cagttgctca aggcatatga tgtcaatatc
     7801 tccggtctgg taagcacaac catgcagaat gaagcccgtc gtctgcgtgc cgaacgctgg
     7861 aaagcggaaa atcaggaagg gatggctgag gtcgcccggt ttattgaaat gaacggctct
     7921 tttgctgacg agaacaggga ctggtgaaat gcagtttaag gtttacacct ataaaagaga
     7981 gagccgttat cgtctgtttg tggatgtaca gagtgatatt attgacacgc ccgggcgacg
     8041 gatggtgatc cccctggcca gtgcacgtct gctgtcagat aaagtctccc gtgaacttta
     8101 cccggtggtg catatcgggg atgaaagctg gcgcatgatg accaccgata tggccagtgt
     8161 gccggtctcc gttatcgggg aagaagtggc tgatctcagc caccgcgaaa atgacatcaa
     8221 aaacgccatt aacctgatgt tctggggaat ataaatgtca ggctccgtta tacacagcca
     8281 gtctgcagtc atggtaccgg cagtgtattc tgccgggcag cctgcatcgc tgcctgttgc
     8341 cattgattat ccggcagctc tggcacttcg ccagatgtcg atggtccatg atgaactgcc
     8401 aaaatatctg ctggctccgg aagtgagtgc cctgctccat tacgtcccgg atctgcaccg
     8461 caagatgctg ctggccacac tgtggaacac cggagcacgc attaatgaag cactggcgct
     8521 gacgcggggg gatttttcgc ttgcgcctcc gtatccgttt gtgcagcttg cgaccctgaa
     8581 acaacggacc gaaaaagctg ccaggacggc ggggagaatg cccgccggtc agcagactca
     8641 ccggctggtt ccgctctctg actcctggta cgtcagccag ctgcagacga tggtggcaac
     8701 actgaaaata cccatggagc ggcgtaaccg tcgcacagga aggacagaga aagcgcggat
     8761 ctgggaagtg acggacagaa cggtcaggac ctggattggg gaggcggttg ccgccgctgc
     8821 tgctgacggt gtgacgttct ctgttccggt cacaccacat acgttccgcc attcctatgc
     8881 gatgcacatg ctgtatgccg gtataccgct gaaagttctg caaagcctga tgggacataa
     8941 gtccatcagt tcaacggaag tctacacgaa ggtttttgcg ctggatgtgg ctgcccggca
     9001 ccgggtgcag tttgcgatgc cggagtctga tgcggttgcg atgctgaaac aattatcctg
     9061 agaataaatg ccttggcctt tatatggaaa tgtggaactg agtggatatg ctgtttttgt
     9121 ctgttaaaca gagaagctgg ctgttatcca ctgagaagcg aacgaaacag tcgggaaaat
     9181 ctcccattat cgtagagatc cgcattatta atctcaggag cctgtgtagc gtttatagga
     9241 agtagtgttc tgtcatgatg cctgcaagcg gtaacgaaaa cgatttgaat atgccttcag
     9301 gaacaataga aatcttcgtg tggtgttacg ttgaagtgga gcggattatg tcagcaatgg
     9361 acagaacaac ctaatgaaca cagaaccatg atgtggtctg tccttttaca gccagtagtg
     9421 ctcgccgcag tcgagcgaca gggcgaagcc ctcgagtgag cgaggaagca ccagggaaca
     9481 gcacttatat attctgctta cacacgatgc ctgaaaaaac ttcccttggg gttatccact
     9541 tatccacggg gatattttta taattatttt ttttatagtt tttagatctt cttttttaga
     9601 gcgccttgta ggcctttatc catgctggtt ctagagaagg tgttgtgaca aattgccctt
     9661 tcagtgtgac aaatcaccct caaatgacag tcctgtctgt gacaaattgc ccttaaccct
     9721 gtgacaaatt gccctcagaa gaagctgttt tttcacaaag ttatccctgc ttattgactc
     9781 ttttttattt agtgtgacaa tctaaaaact tgtcacactt cacatggatc tgtcatggcg
     9841 gaaacagcgg ttatcaatca caagaaacgt aaaaatagcc cgcgaatcgt ccagtcaaac
     9901 gacctcactg aggcggcata tagtctctcc cgggatcaaa aacgtatgct gtatctgttc
     9961 gttgaccaga tcagaaaatc tgatggcacc ctacaggaac atgacggtat ctgcgagatc
    10021 catgttgcta aatatgctga aatattcgga ttgacctctg cggaagccag taaggatata
    10081 cggcaggcat tgaagagttt cgcggggaag gaagtggttt tttatcgccc tgaagaggat
    10141 gccggcgatg aaaaaggcta tgaatctttt ccctggttta tcaaacgtgc gcacagtcca
    10201 tccagagggc tttacagtgt acatatcaac ccatatctca ttcccttctt tatcgggtta
    10261 cagaaccggt ttacgcagtt tcggcttagt gaaacaaaag aaatcaccaa tccgtatgcc
    10321 atgcgtttat acgaatccct gtgtcagtat cgtaagccgg atggctcagg catcgtctct
    10381 ctgaaaatcg actggatcat agagcgttac cagctgcctc aaagttacca gcgtatgcct
    10441 gacttccgcc gccgcttcct gcaggtctgt gttaatgaga tcaacagcag aactccaatg
    10501 cgcctctcat acattgagaa aaagaaaggc cgccagacga ctcatatcgt attttccttc
    10561 cgcgatatca cttccatgac gacaggatag tctgagggtt atctgtcaca gatttgaggg
    10621 tggttcgtca catttgttct gacctactga gggtaatttg tcacagtttt gctgtttcct
    10681 tcagcctgca tggattttct catacttttt gaactgtaat ttttaaggaa gccaaatttg
    10741 agggcagttt gtcacagttg atttccttct ctttcccttc gtcatgtgac ctgatatcgg
    10801 gggttagttc gtcatcattg atgagggttg attatcacag tttattactc tgaattggct
    10861 atccgcgtgt gtacctctac ctggagtttt tcccacggtg gatatttctt cttgcgctga
    10921 gcgtaagagc tatctgacag aacagttctt ctttgcttcc tcgccagttc gctcgctatg
    10981 ctcggttaca cggctgcggc gagcgctagt gataataagt gactgaggta tgtgctcttc
    11041 ttatctcctt ttgtagtgtt gctcttattt taaacaactt tgcggttttt tgatgacttt
    11101 gcgattttgt tgttgctttg cagtaaattg caagatttaa taaaaaaacg caaagcaatg
    11161 attaaaggat gttcagaatg aaactcatgg aaacacttaa ccagtgcata aacgctggtc
    11221 atgaaatgac gaaggctatc gccattgcac agtttaatga tgacagcccg gaagcgagga
    11281 aaataacccg gcgctggaga ataggtgaag cagcggattt agttggggtt tcttctcagg
    11341 ctatcagaga tgccgagaaa gcagggcgac taccgcaccc ggatatggaa attcgaggac
    11401 gggttgagca acgtgttggt tatacaattg aacaaattaa tcatatgcgt gatgtgtttg
    11461 gtacgcgatt gcgacgtgct gaagacgtat ttccaccggt gatcggggtt gctgcccata
    11521 aaggtggcgt ttacaaaacc tcagtttctg ttcatcttgc tcaggatctg gctctgaagg
    11581 ggctacgtgt tttgctcgtg gaaggtaacg acccccaggg aacagcctca atgtatcacg
    11641 gatgggtacc agatcttcat attcatgcag aagacactct cctgcctttc tatcttgggg
    11701 aaaaggacga tgtcacttat gcaataaagc ccacttgctg gcctgggctt gacattattc
    11761 cttcctgtct ggctctgcac cgtattgaaa ctgagttaat gggcaaattt gatgaaggta
    11821 aactgcccac cgatccacac ctgatgctcc gactggccat tgaaactgtt gctcatgact
    11881 atgatgtcat agttattgac agcgcgccta acctgggtat cggcacgatt aatgtcgtat
    11941 gtgctgctga tgtgctgatt gttcccacgc ctgctgagtt gtttgactac acctccgcac
    12001 tgcagttttt cgatatgctt cgtgatctgc tcaagaacgt tgatcttaaa gggttcgagc
    12061 ctgatgtacg tattttgctt accaaataca gcaatagtaa tggctctcag tccccgtgga
    12121 tggaggagca aattcgggat gcctggggaa gcatggttct aaaaaatgtt gtacgtgaaa
    12181 cggatgaagt tggtaaaggt cagatccgga tgagaactgt ttttgaacag gccattgatc
    12241 aacgctcttc aactggtgcc tggagaaatg ctctttctat ttgggaacct gtctgcaatg
    12301 aaattttcga tcgtctgatt aaaccacgct gggagattag ataatgaagc gtgcgcctgt
    12361 tattccaaaa catacgctca atactcaacc ggttggagat acttcgttat cgacaccagc
    12421 tgccccgatg gtggattcgt taattgcgcg cgtaggagta atggctcgcg gtaatgccat
    12481 tactttgcct gtatgtggtc gggatgtgaa gtttactctt gaagtgctcc ggggtgatag
    12541 tgttgagaag acctctcggg tatggtcagg taatgaacgt gaccaggagc tgcttactga
    12601 ggactcactg gatgatctca tcccttcttt tctactgact ggtcaacaga caccggcgtt
    12661 cggtcgaaga gtatctggtg tcatagaaat tgccgatggg agtcgccgtc gtaaagctgc
    12721 tgcacttacc gaaagtgatt atcgtgttct ggttggcgag ctggatgatg agcagatggc
    12781 tgcattatcc agattgggta acgattatcg cccaacaagt gcttatgaac gtggtcagcg
    12841 ttatgcaagc cgattgcaga atgaatttgc tggaaatatt tctgcgctgg ctgatgcgga
    12901 aaatatttca cgtaagatta ttacccgctg tatcaacaca gccaaattgc ctaaatcagt
    12961 tgttgctctt ttttctcacc ccggtgaact atctgcccgg tcaggtgatg cacttcaaaa
    13021 agcctttaca gataaagagg aattacttaa gcagcaggca tctaaccttc atgagcagaa
    13081 aaaagctggg gtgatatttg aagctgaaga agttatcact cttttaactt ctgtgcttaa
    13141 aacgtcatct gcatcaagaa ctagtttaag ctcacgacat cagtttgctc ctggagcgac
    13201 agtattgtat aagggcgata aaatggtgct taacctggac aggtctcgtg ttccaactga
    13261 gtgtatagag aaaattgagg ccattcttaa ggaacttgaa aagccagcac cctgatgcga
    13321 ccacgtttta gtctacgttt atctgtcttt acttaatgtc ctttgttaca ggccagaaag
    13381 cataactggc ctgaatattc tctctgggcc cactgttcca cttgtatcgt cggtctgata
    13441 atcagactgg gaccacggtc ccactcgtat cgtcggtctg attattagtc tgggaccacg
    13501 gtcccactcg tatcgtcggt ctgattatta gtctgggacc atggtcccac tcgtatcgtc
    13561 ggtctgatta ttagtctggg accatggtcc cactcgtatc gtcggtctga ttattagtct
    13621 gggaccacgg tcccactcgt atcgtcggtc tgattattag tctgggacca cggtcccact
    13681 cgtatcgtcg gtctgattat tagtctggga ccacgatccc actcgtgttg tcggtctgat
    13741 tatcggtctg ggaccacggt cccacttgta ttgtcgatca gactatcagc gtgagactac
    13801 gattccatca atgcctgtca agggcaagta ttgacatgtc gtcgtaacct gtagaacgga
    13861 gtaacctcgg tgtgcggttg tatgcctgct gtggattgct gctgtgtcct gcttatccac
    13921 aacattttgc gcacggttat gtggacaaaa tacctggtta cccaggccgt gccggcacgt
    13981 taaccgggct gcatccgatg caagtgtgtc gctgtcgccg gcctcctcac ccggtcacgt
    14041 ttcgtcgttc ctcctccacg cgctgcggct tcggggccgc acctgcattc gtatgcggtc
    14101 gcccggttac aggtgcggca cggcctgatg gaggccgcat gtgagaggag aattcccatg
    14161 ccaaactggt gctcgaatcg tatgcatttt tctggtgaac cggcacagat tgctgagatt
    14221 aaacgactgg ccagcggtgc cgtcacaccg ctttatcgcc gcgccacaaa tgaaggtatt
    14281 cagctgtttc tggccggaag tgccggactt ctgcagacca ctgaagatgt gcagtttgaa
    14341 ccgtgccccg gactgacgga tgccggacgt ggtgttgtat cgccggagaa tatcgcgttc
    14401 acccgctggc tgacacacct gcagaacggt gtactgctgg atgagcaaaa ctgcctgatg
    14461 ctgcatgaac tctggctgca gagcggtact ggccagcgtc gctgggaagg attaccggat
    14521 gaggtcaggg aaaccatcac cgtgcatttc accgcaaaaa gaggtgactg gtgtggcttc
    14581 tggagtaacg aggatgtatc ggtgtggtgg aaccgtctgt gtgacaatgt actgccggaa
    14641 aaaaccatgc cgtttgacct gctgacggtt ctgcccaccc gcctggatgt tgaagtgaat
    14701 ggctttaacg gtggtgttct gaacggtgtt ccttctgcgt atcactggta tacggaacag
    14761 tatggcgtga agtggcctgt ggggtatgag gtgaatatca gccgtcaggg agaaaacttc
    14821 attcaagtgg atttcgacac gccgtggtgt cagccggaaa gcaacgttgt tgcagaatta
    14881 agccgccgtt tcggctgcac gctggagcac tggtacgccg aacagggctg caatttctgt
    14941 ggctggcagc gatatgagcg tggagagctt gttgatgtgc tgtgggggga actggaatgg
    15001 tcttccccga cagatgatga tgagctgccg gaagtcaccg cgccggagtg gatagtcgac
    15061 aaggtggcgc attacggggg atgaagtatg acggagacgg gcgggcaacc gcccgtttct
    15121 tttccgacaa aggatgtcgc cgtgctcctc tttttactgc gccggctgac gcggcgcggc
    15181 aggaacgctg cctgtggtca gtgtcccgcg tccggtgggc acgggacggg cgcttttacc
    15241 tgctcccggc tggcgtcggt gacagtgtac gccagcctgt cgcccttttc tgatgaacgc
    15301 ccttcaagcc gctttcgcgg catatcctcg ccgtcaaaaa gacgacggct gcgatattcc
    15361 acggtcggcc tgacccgtta ccggacgcgg tgaacagccc acaggcagcg gggaacgggc
    15421 accacagggg ggccctcccg gtgccctctg taagagaagg agcgttgtta tgtcccgttt
    15481 tgtccttggt aactgcatcg atgttatgac gcgtatccct gataacgcca ttgatttcat
    15541 attaactgac ccgccagtat ctcgtcggtt ttcgtgaccg ttccgggcgc accatcgccg
    15601 gcgataaaac cgatgaatgg ctgcaaccgg cctgtaatga aatgtaccgc gtactcaaaa
    15661 aagacgcgct gatggtgagc ttctacggct ggaaccgcgt cgatcgcttt atggccgcct
    15721 ggaaaaatgc gggattcagc gttgttggtc acctggtctt caccaaaacc tacacatcga
    15781 aggccgcata tgtgggctat cgccacgaat gcgcctacat cctggcaaaa ggccgtccac
    15841 gtctgccaca aacccgctgc cggacgtgct gggctggaaa tattctggca atcgtcatca
    15901 cccgacggaa aagcccgtta ccagcctgca accgctgatt gagagcttca cacacccgaa
    15961 cgcaattgtg ctggacccgt ttgcaggcag cggctcaacc tgcgtcgccg ccctccagtc
    16021 cggacgccgg tatatcggta tcgagctgct tgagcagtat caccgtgccg ggcagcaacg
    16081 ccttgccgcc gtgcaacggg ccatgcagca gggggccgcg aatgatgact ggtttatgcc
    16141 ggaggctgcg taaatgaact atgccggaca cgaaaaactt cgcgccgacg tggcggaggt
    16201 ggccaacacc atgtgtgacc ttcgggccag gctgaacgat atggagcatc gctgtcgctt
    16261 tgattccgat gtgcttgttg aacgcctgac acgccagacg ctgtatcgcg ccaatcgcct
    16321 gttcatggag gcgtataccg aaattctgga actggattcc tgcttcaaag attgagaagg
    16381 agacgagaat gtacggaaca tgcgaaacgc tatgccggga gctggcagca aagtatccgg
    16441 gagacacgcc gctgatgctg gttatctggt ccccggaaga gattcaggcc ctggctgacg
    16501 gaatggatat ttctctgacc gatcatgaaa tcaggaccgt cctggcgcgc ctggaggaca
    16561 tcccggaaga ccagcggact gaatccggta tttcttccgc cgcggtgatg gagattatca
    16621 gcaacgtgag cgaaaaccgc caggtgaccg tccctgctga actgctggcg tccctgattc
    16681 agaccgctga acaggcactg tggaaacgtg agtgggccgc ccgtgataac ggcctcgccg
    16741 tcccggaatg cgttacccgc cgtcaggcgg tggttaatca ggcccgcacc ctgctgaaaa
    16801 acaacacaca cgaaaacaac tgatgttatc gccgcctgcc ctgcgggcgg cgatacaggg
    16861 agaacgctta atcatgaacg aaaacagcac gctgaacgca ctgatttgtc gtcacgcccg
    16921 taacctgctg ctggcgcagg gctggccgga agagacggat gttgaccagc gaaatccgaa
    16981 ctatccgggc tggatcagca tttacgtgcg gctggatgcg ccccggctgg cgacgttact
    17041 tatcaaccgt cacggcggcg tactgccgcc gctcctggcc tccgccattc agagactgac
    17101 cggaaccggg gcggaactgg tactgtccgg cagtcagtgg cagtcgctgc cggtacttcc
    17161 ggcagacgga acgcaggtgt ctttcccgta tgccggagaa tggctgaccg aagacgaaat
    17221 cagggctgtt cttgatgcgg tgcacgatgc aggtacgaag catctgttac caggtggcag
    17281 aagatgcgcg gcgtatccgt gcggcgctga ccaccaccgg tcagacgttg ctgacccgcc
    17341 agacgcgccg ctttcgcctg gtcgtgaagg aaagcgatca cccctgctgg ctcgatgaag
    17401 atgacgaaaa cctgcccgtg gtgctcgatg ccatcgtgaa ccggggcgca cgtttttcgt
    17461 cggtggaaat gtatctggtc agtgagtgtg tggagcatat cctgtccagt gggctggcct
    17521 gcgatgtgct gcgtataccg gatgaaccgc cccgccgctg gtttgaccgt ggtgttctgc
    17581 gggaggtggt ccgggaagcc cggaccgaaa tccgcagcat ggcggatgcc ctggcaaaaa
    17641 tccggaaatg atgacactgg cggggtaaaa tccccgcctt tttcctgccg gtccccggac
    17701 agagcacaaa ggaagtcgcc gccggctccg ctttacccgg ccatgcaggg catggccttg
    17761 tggtttttca gttatgtggc ctcagcgtcg tgtgcgggct gtgccgtgcc tccatcttag
    17821 ccgggctggc gttgatgcaa gggtacgctt cgccgctgcg gtcacccggt ccctccttcc
    17881 cgtctgccgt gattttccgg tcgttcaccc gctcagattt cgcggtctca ccctgcaact
    17941 cccgtcagcc gtgtgcgtca ggctgcggct tcccttgcgc ccctgcatcc ccgcctgttc
    18001 gcccggcttt tatggaggca cggcaccgcc cggtgtcgcg gaatatgtga acaacggagg
    18061 aaaacatcat gcaatatgcg aaacctgtca ctctgaatgt tgaagagtgc gaccgtctct
    18121 cctttctgcc gtatctgttt ggcaacgatt ttctgtatgc cgaggcgtat gtgtacgcgc
    18181 tggcgcaaaa aatgatgccg gaatatcagg gaggcttctg gcacttcatc cgcctgccgg
    18241 acggtggcgg ttacatgatg ccggatggcg accgttttca tcatggtgaa cggtgcaaac
    18301 tggtttgacc gtaccgtgag tgctgatgcc gcaggcatca tcctgacctc ccttgtgatt
    18361 aaccgccagt tgtggctgta ccacgacagc ggggatgcag gactgaccca gctttaccgg
    18421 atgcgcgatg cgcagttgtg gcgtcacatc gaatttcacc ccgaatgcaa cgcgatttac
    18481 gcggcatctg gactgattaa cggacggggc ggcaatgccg cccctgtaaa ggagacgaga
    18541 acatgtactg tactgttaaa gaaattcatc cgcgaagtgc tggatacaga cgtgccggac
    18601 agtgaatgcg tttttgccgt agtgctgacc cgtggggatg tgcgtcacat agcccaggac
    18661 tggagtctga cagacgatga gctggaaacc gtcatgcagc ggctggacga tgccttttgt
    18721 aatatggtgc ggatgtgcag agttgtttca cggcgttgta agtgaactga tggaagaaaa
    18781 gcgcgccagc cgtcaggtga cagtcccggc ggtgatgctg gaaaaagtga tggcgctggc
    18841 aggcagtgaa atgaagcgcc tgtatgccgt cgggagtgag aacgggggcg acggtgacgc
    18901 attcgtcagg gaggaacgcg aagcaatgga cgttgtgtta caggcgctgg acggggagca
    18961 catgtcatga atatcagcac agaaacccgc gaaattctgc gtaattacag ggccgtgatt
    19021 aatgcgcggc gtcgtgaaat ggggcagaaa ccgctcacca ccgcgcagat tgttgatgaa
    19081 atctgcgatt ttgtggcgaa tcagcaggcg gttttcctgg gcggtcacta catcttacag
    19141 ggtagcagaa acaggtgata caggggcagg cggtggaggc cgcctgtcag cccgcgcgaa
    19201 accccctggc aggctgacgc ctgccggaag ggaagcgggc cacgctggcg cgtcgcccgt
    19261 tacccttccc gtctgtgatg tgtctgactc aggagtgttt ctttcttatg ttatggaact
    19321 gttcctgatg ttgttgttcc tttatctgct gctctgattc tgactactgc agtgttgtcc
    19381 tgtctgccgg gcgcaccttc ggctcccggc tgcctggcgg catccggtcg tcaggcccga
    19441 ctccgacagg cagctgctgg cgcacctgcc agtctccgcc gaacccgctg gcgcggtttc
    19501 gggctgtcgc tacggtccga tgcgtgaaaa ccccgggctt gcagcgaccg ttccggtcgc
    19561 gccacccttg tcgcgccgtg gcctgcgctc ccggcatccc ggcaaaaggc cgccgggatg
    19621 taaggggcaa cgccgtttcg cgcacgcgct ctccgtcgtt gcggcgggtc cctgctcatt
    19681 gcctgccggt gccacacacc cgtatgagtc cctgtcgccg ggggctgata cagccacaac
    19741 ctgccgcact gactgtccag ggtaaatgaa cagcccacaa aattttttac ccaaaacccg
    19801 gtaaaaaatt ttgcgttctg cccaggacag gtgcgtcagg ccgtggcagt gatgcccctt
    19861 gcggcgaagg ttctcaaacg ggtgaagttc ataccacagg gtaatacagc agcaggttcc
    19921 cgcgaaacgt caaaaccgcg gctcccgaaa ctcaacgggt gatgttcttt aaaaatcagg
    19981 atttatttca ggctccggca atacgcattt atgtgttctg tcagtcatcc ggctggcagg
    20041 taatgagaac gcaggaacag aaggaggaaa acaccggaga acgtcagacg cagatatgaa
    20101 aaaagcaggt cagggacctg cttaatccac accgcagaga ctgcacgaac agtctttacg
    20161 gcttcattaa ttaacgtcat aagaggctaa attatgacaa gtaagtattg tttcaccttc
    20221 cgcagcggct gtaaagcccc gccgtcatcc ccgtttcaga cgcgaagaca tccccgcgcc
    20281 ggaaattgac cccgtgctga aagccttcgg gcgacacata gcccggagtt ttcaccgggg
    20341 gcgtggtgta cacatccccg ccatgaaaaa cacggccttc gggcaggtgc tccgcacact
    20401 cgaactgaaa cgcgctttta actgatgagg tgagccgccc cgcgaggggc ggcaacagac
    20461 ggaaggagaa cattttatga cattagcagc aatcaccatg accgccccgg aagccgccag
    20521 ccctgtgcag atgtaccgcg cgacctactc accggatgac aacaaactgc gcctgtatgc
    20581 cgtgtcacgt cttgacccgg agacgtataa aaaagtgcat gatgccggtt ttccgctggg
    20641 caccaaaaca ggcgctgttt gtcgcgccag cctggacacc gggccgggaa gacgtgctcc
    20701 tctcacttgc cggagagatt gaggatgaag acagcacgct cgctgaacgt caggaagcac
    20761 gggcggagcg gtttaccgga tacagcggaa agcgggccag tgaatccgca caggcacttg
    20821 atgaagtgga aagactggcc gcgatgatcc cgcccggtca gcccattctt gtggggcatc
    20881 acagcgaacg ccgcgcccgt cgtgatgcgc agcgtattga aaacggcatg aaacgtgccg
    20941 tgatgctctt tgaacgtgcg gaatactggg aagaacgggc gcggtcggca ctgcttcacg
    21001 cgaagtataa agaacgtccg gacgttcgct ggcgtcgtat caaaaaaatc gaagctgatt
    21061 tgcgcaaggc tgaaaagacc atcgcacagt cgcagaaata tctgacgatg tggcgggctg
    21121 aatcgctgga tctgaatatg gcaaaactca tcagcagtca tgaccatatc agcgcctgtt
    21181 tcccgctgga tacgtatccg cgcccggcag aaaaaagcca gtatgaagga agtcgatcgt
    21241 tatggtcggc cctggatgat gacatcatca ccacggagca ggcccgcgaa attgcgatcc
    21301 gctgtcatga acggcagatt cagcatcagc aacgctgggt taaccactat cagaaccgcc
    21361 tgatctatga gcgtgccatg ctggacgaaa gcggcggcgt ggttacccgg acacaggatt
    21421 ttgagccggg cggacaggtt ttcagccggg gcgagtggct gaccatcatc cgcgtgaaca
    21481 aaagcaacgg ggcggtgagt tcagtcacaa cgccgaatta cagttttctc gggtacagcg
    21541 gcacgatgaa agtgacgccc gatcgcatca cggactacaa agcaccatcg gcagaagagg
    21601 ctgccgtcgc cagtccaggc cgcgaagcgt ccgccggtag tcaactatcc gggggaaggt
    21661 ttccgggaaa tgacaaaggc gcagtgggcc gccctgcccc gggactgtaa ggccgtgcgc
    21721 agtgtggcag agacagaaga ccacggggca taccgctacc gccgcacaat ggacaataat
    21781 ttccgcctgg tgaatgtgta tatcaccgac atgaaaatca cggaaatccc gcagaaataa
    21841 ggtatatccc cccgggaaat ccccggggga tgacggagat aatgatatgc acagtcagtt
    21901 aaaagaacgt atccggctga tgcgcgcaag gctggataac gccgcgccgg ttgctgaaat
    21961 ccgggctgaa tctcagcttt ttgtgactcc tgccccggtc tgtgatcgcc tggtgacgct
    22021 ggcggagatc agcaaccgtg atcacattct ggaaccctct gccggcaccg gggccatact
    22081 gcgggcgatt cgtcatgtcg cgccgggggc catgtgtgat gcggtggaga ttaacagcgg
    22141 gctggtccgg tatctgcggg ataattttaa cggtgtcagg gttcagtgtg gcgacttcat
    22201 ggaatggcag ccggtgcagt attacagccg gattatcatg aatccgccgt tcagtcacgg
    22261 gcaggatatc cggcatatcc tgcgggcttt ttccctgttg cgtccgggtg gtgtgctggt
    22321 tgccgtctgt ctgaacgggc cgcgccagca ggagaagctg ttaccgtttt ctgatcgtcc
    22381 gcgaggagct gccgtgcggc acatttgctt atacccgtgt tccgacgatg attattcgtc
    22441 tgcgtgcctg atgactccgg gcaggcggga ggataaatgc ccgtctgccc gcgccgggaa
    22501 aaccgcgcgc cggtggcgct gaaaggggcc gcgcttacgc gccgcccctt tcattacgcg
    22561 gacgttctgc tgtgggcttc tgtcttttca gacctgtcat ttgcctgtta ctgatgatgt
    22621 ttttgttcct gctactgcta ctgctactgc tactgttctt cttttttctg ctgctctgat
    22681 tactgtctgt tttgttgttc tcctgcttat gccttttccg ggcgtccccc tggcggggcg
    22741 ggctgtcgtg aacggagcca gcggagctgt ctccgccctg gcgggcttcc atccctgacg
    22801 caaaacccgg gtttcagcga ccggaacggt cgcgccaccc tgtcgcgccg tggcctgcgc
    22861 tcccggcatc
//
