LOCUS       NZ_CP069541             4546 bp    DNA     linear   CON 10-JAN-2024
DEFINITION  Acinetobacter pittii strain FDAARGOS_1214 plasmid unnamed4.
ACCESSION   NZ_CP069541
VERSION     NZ_CP069541.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN16357356
            Assembly: GCF_016889825.1
KEYWORDS    RefSeq.
SOURCE      Acinetobacter pittii
  ORGANISM  Acinetobacter pittii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Acinetobacter; Acinetobacter calcoaceticus/baumannii
            complex.
REFERENCE   1  (bases 1 to 4546)
  AUTHORS   Sproer,C., Gronow,S., Severitt,S., Schroder,I., Tallon,L.,
            Sadzewicz,L., Zhao,X., Boylan,J., Ott,S., Bowen,H., Vavikolanu,K.,
            Mehta,A., Aluvathingal,J., Nadendla,S., Lowell,S., Myers,T., Yan,Y.
            and Sichtig,H.
  TITLE     FDA dAtabase for Regulatory Grade micrObial Sequences (FDA-ARGOS):
            Supporting development and validation of Infectious Disease Dx tests
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4546)
  AUTHORS   Sproer,C., Gronow,S., Severitt,S., Schroder,I., Tallon,L.,
            Sadzewicz,L., Zhao,X., Boylan,J., Ott,S., Bowen,H., Vavikolanu,K.,
            Mehta,A., Aluvathingal,J., Nadendla,S., Lowell,S., Myers,T., Yan,Y.
            and Sichtig,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2021) Institute for Genome Sciences, University of
            Maryland School of Medicine, BioPark II, 801 W. Baltimore St., Suite
            619, Baltimore, MD 21201, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: SMRT v. 8.0.0, HGAP v. 4, SPAdes v. 3.14.1
            Genome Coverage                   :: 1835.78x
            Sequencing Technology             :: Pacbio; Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/10/2024 01:54:04
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,952
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,766
            CDSs (with protein)               :: 3,766
            Genes (RNA)                       :: 96
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 41 of 90
            Pseudo Genes (incomplete)         :: 35 of 90
            Pseudo Genes (internal stop)      :: 28 of 90
            Pseudo Genes (multiple problems)  :: 11 of 90
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP069541.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..4546
                     /organism="Acinetobacter pittii"
                     /mol_type="genomic DNA"
                     /strain="FDAARGOS_1214"
                     /culture_collection="DSM:9306"
                     /db_xref="taxon:48296"
                     /plasmid="unnamed4"
                     /geo_loc_name="Germany: Braunschweig"
                     /collected_by="DSMZ"
     gene            complement(<1..565)
                     /locus_tag="I6J44_RS19615"
                     /old_locus_tag="I6J44_19615"
     CDS             complement(<1..565)
                     /locus_tag="I6J44_RS19615"
                     /old_locus_tag="I6J44_19615"
                     /inference="COORDINATES: protein motif:HMM:NF021564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2290 domain-containing protein"
                     /protein_id="WP_204086202.1"
                     /translation="MDSDAISKQCTEIIDRLILSGLSIEQSYPSTRMNASGDYEIGFKK
                     GIEISQTLKNIPYEDIYKSVLDEKSYHIHLIDGGLVSFNYIFEKNDDVFFIKKHRLSFY
                     PSPSLPSFEDCPELYSNDEIFLECTKKNLVKFPIRFDFDPKNHVNVTHPASHVTFRQFL
                     NCRIPVSMPVTPRKFILFLLRNFYY"
                     /name="DUF2290 domain-containing protein"
     gene            complement(567..2705)
                     /locus_tag="I6J44_RS19620"
                     /old_locus_tag="I6J44_19620"
     CDS             complement(567..2705)
                     /locus_tag="I6J44_RS19620"
                     /old_locus_tag="I6J44_19620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007355371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding domain-containing protein"
                     /protein_id="WP_204086203.1"
                     /translation="MIKVIWGTNKEKPIASRQLAETLSSNMSLEGFLYIGYPIVGSPLG
                     PTKFDALLLSESKGIIAFDLVEGTDLTNYINNQDEMASMLEVKLKPYPKLKKGRHLKFD
                     IKTVTFCPAKKNNLPQVEDNLYNIANISNLNEVINSFEWESDEDTFKELKAAIQVVTNI
                     RSGAIRRQTKKENSLGSRLQKLEDSIANLDQHQSEAVIETVDGVQRSRGLAGSGKTIVL
                     ALKVAYLHAQNQSWKIAVTFHTRALKEQFKRLINNFTIEQLGSEPDWDSIDIFNSWGAP
                     GDKENDGMYHRYCVENSIDYYDFQTAKNEFGGDDAFKNVCKLALEQSSSNKIEKYDLIL
                     IDEAQDFPPEFIKLCYKFLKEPKRLVYAYDELQSLNGLSVLPPEQLFGKDSKGVPLVTL
                     EEEDPTKPKKDIILEKCYRNSRPLLATAHTLGFGLTRPKGLIQFFDNDSLWEDVGYSVK
                     KGKIEGGKEVELIRTSESSPLFLEEHTPLEELIKFEVFDSVRDMNQMLFDSIVKNIHED
                     ELKPEDILVINPDPFTTQKNVGIVRKALQQNEIDSEIAGVTTSRDIFRKNGSVTFTGIF
                     RAKGNEAAMVYIIHAQDCADSYDPNHLALIRNRLFTAITRSKAWVRVLGIGPSMQALKE
                     EWETLKNNDYALKFVYPTEKQKNTLKLINKDMSVQERNRRRKLTHNIQEIIHAINSGEL
                     PTELIPEELINILKKSGH"
                     /name="ATP-binding domain-containing protein"
     gene            complement(2928..3242)
                     /locus_tag="I6J44_RS19625"
                     /old_locus_tag="I6J44_19625"
     CDS             complement(2928..3242)
                     /locus_tag="I6J44_RS19625"
                     /old_locus_tag="I6J44_19625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006582675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnA antitoxin family protein"
                     /protein_id="WP_057095489.1"
                     /translation="MSMVKYSRKELNEKFSDKQDAEIERLLAKGTVSDDHLDLSDMPEI
                     TDWSNAVRHNQFYRPVKQQTSIRLDADVLAWFKAQGKGYQTRMNEILRDAMLKELKNHQ
                     "
                     /name="BrnA antitoxin family protein"
     gene            complement(3229..3516)
                     /locus_tag="I6J44_RS19630"
                     /old_locus_tag="I6J44_19630"
     CDS             complement(3229..3516)
                     /locus_tag="I6J44_RS19630"
                     /old_locus_tag="I6J44_19630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019383542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnT family toxin"
                     /protein_id="WP_039890878.1"
                     /translation="MEQYFEWDEAKNRKNQKKHDVSFETASLVFEDPLRISIQDRHTDG
                     EERWQTIGKVKGVLMLLVAHTIFDEDDCEIIRIISARQVTKAERDKYEHG"
                     /name="BrnT family toxin"
     gene            complement(3766..4095)
                     /locus_tag="I6J44_RS19885"
     CDS             complement(3766..4095)
                     /locus_tag="I6J44_RS19885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004785588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_005237346.1"
                     /translation="MKNLQIVIAQALHSYEFEHMEQSISKSISDTILEILVNDGPFEKI
                     DCYLYSKKDSKKILKSSIHYVIAVLERAKYRVQKAQQDNLSPLYICLYVIKKIEGFIEY
                     LNTKF"
                     /name="hypothetical protein"
     gene            <4214..4348
                     /locus_tag="I6J44_RS19635"
                     /old_locus_tag="I6J44_19635"
                     /pseudo=""
     CDS             <4214..4348
                     /locus_tag="I6J44_RS19635"
                     /old_locus_tag="I6J44_19635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004733157.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /name="cysteine hydrolase"
ORIGIN
        1 aataataaaa attacgaagt aaaaatagaa taaattttct tggagtaact ggcattgaaa
       61 caggtattct acaattgaga aattgcctaa atgttacatg cgatgcagga tgagtaacat
      121 tcacatgatt tttgggatca aagtcgaatc taatgggaaa tttaactaaa ttttttttag
      181 tacactctaa gaaaatttca tcattactat aaagttctgg gcaatcttca aaggatggca
      241 atgatggtga aggatagaat gacaatctat gtttttttat aaaaaaaaca tcatcatttt
      301 tctcaaaaat ataattaaaa gaaactaagc ctccatctat caggtgaata tgatagcttt
      361 tttcatctaa aacagactta tatatatctt cataagggat attttttagc gtttgagaaa
      421 tttcaatacc ttttttaaat ccaatttcat aatctccact cgcattcata cgtgtggatg
      481 gatagctttg ttctattgat aacccagata aaataaggcg gtcaatgatt tcagtacatt
      541 gtttagaaat agcatcagaa tccatcttaa tgccctgatt tttttaaaat attaattaat
      601 tcttcaggaa ttaattcagt tggtaattct cctgaattga tagcatgtat aatttcttga
      661 atattatgtg tcagttttct tcgtctattc ctctcttgaa cagacatatc tttattaatt
      721 aactttaatg tatttttctg cttttctgtc ggataaacaa actttaaagc ataatcgttg
      781 ttttttagag tttcccattc ttcttttaga gcctgcatag atggcccaat tcccaagaca
      841 cgaacccaag ctttactccg agtaatagca gtaaataatc tatttctgat tagtgctaag
      901 tgatttggat cataggaatc tgcacaatct tgagcgtgga taatgtagac cattgcagcc
      961 tcattacctt ttgctctgaa gatacctgta aaagtaacag atccattttt tctgaagata
     1021 tctctggagg ttgtgacacc tgcaatttca gaatctatct cattttgttg taatgctttt
     1081 cttacaatcc ctacgttttt ctgtgttgtg aaagggtcag gattaataac taagatatct
     1141 tcaggcttaa gctcatcttc atggatattt ttgacaatag aatcgaaaag catttgattc
     1201 atatctcgaa ctgaatcaaa tacctcaaat tttataagtt cttcaagagg tgtatgttct
     1261 tccaaaaata gagggcttga ttcactagtt ctaattaact ctacttcttt tccaccttca
     1321 attttacctt ttttaaccga ataaccaaca tcttcccata gagaatcatt atcaaaaaat
     1381 tgaattaatc cttttggtct agttaatcca aaccctagtg tatgagcggt tgcaagtagt
     1441 ggacgtgaat tgcggtaaca tttttcgaga ataatatctt ttttaggttt tgttggatct
     1501 tcttcttcta aggttactaa tggcactcct ttggaatctt tcccaaataa ctgttcagga
     1561 ggtaaaacgg acagaccatt taaagactga agttcatcat atgcataaac taatcgctta
     1621 ggttccttta aaaatttata acataactta atgaactcag gagggaagtc ttgagcttca
     1681 tcaatcaaaa ttaaatcata tttttctatt ttattagatg aggactgttc taaagctagt
     1741 ttacaaacat ttttgaaagc atcatcccct ccgaactcat ttttagccgt ttggaaatca
     1801 tagtaatcta ttgagttttc aacacaatat cggtgataca tcccatcgtt ttctttatct
     1861 ccgggtgctc cccatgagtt aaatatatca atagaatccc aatcaggttc gctacctaat
     1921 tgttcaatag taaagttgtt gattagcctt ttaaactgtt cttttaacgc acgtgtatgg
     1981 aaagttactg ctattttcca actttggttc tgtgcatgta aataagcaac ttttaacgct
     2041 aatacgatag tttttcctga tcctgctaac cctctgctcc gttgtacacc atccactgtt
     2101 tcaatgactg cttcagactg gtgctgatct aggttcgcta tagaatcttc taatttttgt
     2161 aggcgacttc ctaaagaatt ttccttttta gtttgtcttc taatagcacc agatcgaata
     2221 ttagtaacaa cttgaattgc tgcttttaat tctttaaacg tatcttcatc tgattcccac
     2281 tcaaatgaat tgataacttc atttagattg gaaatattag cgatgttgta tagattatcc
     2341 tcgacttgtg gcaaattatt ttttttagcg ggacagaaag ttacggtttt aatatcaaac
     2401 ttaagatgac gacctttttt taacttggga taaggtttta atttcacctc caacatggag
     2461 gccatttcat cttgattatt tatatagttt gtaaggtccg ttccttccac taaatcaaaa
     2521 gcaatgatcc cttttgactc gcttaaaagt agtgcatcaa attttgtcgg tcctagagga
     2581 gagcctacaa ttggataacc tatataaaga aagccttcta aactcatgtt agaagataat
     2641 gtttccgcaa gttgtcttga agcaattggt ttttctttat tagtgcccca aataacttta
     2701 atcacagcaa tacctttatt taaaatcttg aaatcaagca atgctctact atatttataa
     2761 gccttagtct tatcaagtta agcattgata tgaataactc ttatggttag ttcgaaatca
     2821 aataaactta aacatctcaa tttagagatt gcttattatt aaaaatatct aaaattaata
     2881 taatacacgt tatacgcagt caaaaatggg agcctaagct cccattttta ttgatgattt
     2941 tttaattctt ttagcatagc atcccgtaag atttcattca tacgtgtctg ataacctttg
     3001 ccttgtgctt tgaaccatgc aagtacgtca gcatctaaac gaatagatgt ttgttgcttc
     3061 actggacgat agaattgatt gtggcgtaca gcattactcc agtcggtaat ttcaggcata
     3121 tctgatagat ctagatgatc gtcagaaact gttcccttag caagcaagcg ttcaatttca
     3181 gcatcttgct tgtcgctgaa tttttcattc agttctttgc gtgagtattt aaccatgctc
     3241 atatttatct cgctctgctt tagttacttg tcttgcacta atgattcgta tgatttcaca
     3301 gtcatcttca tcaaagatgg tgtgagctac taagagcatt agtacgcctt ttactttccc
     3361 aatggtctgc caacgttctt cgccatcggt atgtctgtct tggattgaga tcctcaacgg
     3421 atcttcaaaa acaaggcttg cagtttcgaa agagacatcg tgctttttct gattctttcg
     3481 attcttagcc tcatcccatt caaaatactg ttccataaaa aacattcaaa tttgtatata
     3541 caataatgta tcgcaaaatt gtatcactca agctgaaaag cgtaattttt agtcaaattt
     3601 attggcaaaa tatttcaaaa ctgctcataa aaaagccgac ttgtttcaaa atcggctttt
     3661 tgtgcatttt ttggctagag ccaaaatttt aagtatctga tatttatatt aatatacaag
     3721 tgtacttcgt ataatcgcca ttatgttaaa tggaggcttt cacagttaaa atttagtatt
     3781 taagtattcg ataaagccct caattttctt aatcacgtat aagcagatat acaaaggact
     3841 cagattatct tgctgagctt tctgtactct atacttcgcc ctttctaaga cagcaattac
     3901 atagtgtata gagcttttta gaattttttt agaatctttt tttgaataca gataacaatc
     3961 aattttttca aaaggcccat cattgaccaa tatttccaat atagtgtcgc taatggattt
     4021 acttattgat tgctccatat gttcaaattc atatgaatgt agagcttgtg ctataactat
     4081 ttgaagattc ttcattatga ttatttaatg ttagccaatt ttgatcctag cattatctcc
     4141 tgaaaagaaa aagcgtataa aacctaaagt aaacattgag ttatttttat tcttcgttat
     4201 gaaaagttaa aaaaatgatt ctagggccag agctgacttg gaatttaatg gcgttaaagt
     4261 acctgctggt catgtccatg caacattaat ggccgctttt gaatttgcat atgctcaagt
     4321 aatatcaact gaagattatg ttggctaaag cttaagattt cctaaaatta acataataca
     4381 ccttatacga aatgcgttat aattctcttg aaaattcaac gctgtagttt cttttttgcc
     4441 caacctcccc aaggtgggct ttttttttta tgatttttca cgttccacgc ctattatttg
     4501 atcaatattt cacgacttta ttcatattag ttaaacaatc gtggtt
//
