LOCUS       NZ_CP069539             4178 bp    DNA     linear   CON 10-JAN-2024
DEFINITION  Acinetobacter pittii strain FDAARGOS_1214 plasmid unnamed2.
ACCESSION   NZ_CP069539
VERSION     NZ_CP069539.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN16357356
            Assembly: GCF_016889825.1
KEYWORDS    RefSeq.
SOURCE      Acinetobacter pittii
  ORGANISM  Acinetobacter pittii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Acinetobacter; Acinetobacter calcoaceticus/baumannii
            complex.
REFERENCE   1  (bases 1 to 4178)
  AUTHORS   Sproer,C., Gronow,S., Severitt,S., Schroder,I., Tallon,L.,
            Sadzewicz,L., Zhao,X., Boylan,J., Ott,S., Bowen,H., Vavikolanu,K.,
            Mehta,A., Aluvathingal,J., Nadendla,S., Lowell,S., Myers,T., Yan,Y.
            and Sichtig,H.
  TITLE     FDA dAtabase for Regulatory Grade micrObial Sequences (FDA-ARGOS):
            Supporting development and validation of Infectious Disease Dx tests
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4178)
  AUTHORS   Sproer,C., Gronow,S., Severitt,S., Schroder,I., Tallon,L.,
            Sadzewicz,L., Zhao,X., Boylan,J., Ott,S., Bowen,H., Vavikolanu,K.,
            Mehta,A., Aluvathingal,J., Nadendla,S., Lowell,S., Myers,T., Yan,Y.
            and Sichtig,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2021) Institute for Genome Sciences, University of
            Maryland School of Medicine, BioPark II, 801 W. Baltimore St., Suite
            619, Baltimore, MD 21201, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: SMRT v. 8.0.0, HGAP v. 4, SPAdes v. 3.14.1
            Genome Coverage                   :: 1835.78x
            Sequencing Technology             :: Pacbio; Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/10/2024 01:54:04
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,952
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,766
            CDSs (with protein)               :: 3,766
            Genes (RNA)                       :: 96
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 41 of 90
            Pseudo Genes (incomplete)         :: 35 of 90
            Pseudo Genes (internal stop)      :: 28 of 90
            Pseudo Genes (multiple problems)  :: 11 of 90
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP069539.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..4178
                     /organism="Acinetobacter pittii"
                     /mol_type="genomic DNA"
                     /strain="FDAARGOS_1214"
                     /culture_collection="DSM:9306"
                     /db_xref="taxon:48296"
                     /plasmid="unnamed2"
                     /geo_loc_name="Germany: Braunschweig"
                     /collected_by="DSMZ"
     gene            complement(195..1097)
                     /locus_tag="I6J44_RS19130"
                     /old_locus_tag="I6J44_19130"
     CDS             complement(195..1097)
                     /locus_tag="I6J44_RS19130"
                     /old_locus_tag="I6J44_19130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005000587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_204086200.1"
                     /translation="MFGIFSSKKQNSLKNPVYLEKFINNAYLELSNSIKAPNELYLFLI
                     EELCGASQGNNDGKQLVGFSRFHEIEYRDALNKESAMDLPNSPLSILNNSVSPQLIKEL
                     GIDEAVKIRCTLIKRLIEANQNTLNSSRLTFAKSNIQMGSSYLSEGEIQAWFDVINSIQ
                     GASKKTILEPDDLTEIITPSNHTAQGKYYDMFKDLEDYLSSLYEQPSHSTFMPLLYALR
                     IAYAGMYSQGICSKADFDAVDQGFFNRVILIGQSISREEQVSFQESSLDKALEWINKYY
                     IVIDRQTSSHLVNTAKSGL"
                     /name="hypothetical protein"
     gene            complement(1372..1917)
                     /locus_tag="I6J44_RS19135"
                     /old_locus_tag="I6J44_19135"
     CDS             complement(1372..1917)
                     /locus_tag="I6J44_RS19135"
                     /old_locus_tag="I6J44_19135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018680119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid replication DNA-binding protein"
                     /protein_id="WP_016803253.1"
                     /translation="MKALSVIELSQLYGMNRQSIYKRINKGDLSKNSDGKIDLSEAIRV
                     FGEPSNKNSNVTTLQSTAVQKETEVDMLKQQVDILKKQLELAHEREVFQREQLEAKDNQ
                     IEAIQRLLEAPKPNMTTFTDQKPGQNIATDPRPEPELKHDGLTTPEQTENKRIPVPEHV
                     EQEPEKRGFWSRFFRPYD"
                     /name="plasmid replication DNA-binding protein"
     gene            complement(1986..2921)
                     /gene="repM"
                     /locus_tag="I6J44_RS19140"
                     /old_locus_tag="I6J44_19140"
     CDS             complement(1986..2921)
                     /gene="repM"
                     /locus_tag="I6J44_RS19140"
                     /old_locus_tag="I6J44_19140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237873.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein RepM"
                     /protein_id="WP_016803254.1"
                     /translation="MPTSELIVKDNALINASYNLDTVEQRLILLAILQARETRTGIDAN
                     TRLRIHASDYMSRFNVNKHAAYKALKTAVTNLFGRQFSYQTIDEKTGKSKKVISRWVQN
                     ISYIDDAATLEVTFTMDVVPLITRLEKQFTSYQLKQVTQLTGKYAIRLYELLIAWREIG
                     KTPIFEVSDFRSKLGLTSDDYPRLDTFKRRVLESAVKQINEHTDIIVKVEQHKEGRSIS
                     GFSFSFKQKRNQNKTIERKKDPNTLDIFSRITDAQRHLFANKLSELPEMGKYSQGTESY
                     QQFAIRIAEMLQDPAKFQELHPYLEKVGYR"
                     /name="repM"
     Typing          complement(1987..2921)
                     /name="rep_cluster_1233"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="98.077"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 cgcccattat gttaaatgat gaaattttag atatttttac taaaaaacga tataggctta
       61 actagaacag taaaagtagc tgagcaaacc tcgatctaag caagatcgag gtatggattt
      121 tagtttttat agctaggaaa tagtaaaaac tttatcttaa atataagcgc ttatgtaaat
      181 tatagactac tatcttacaa gcctgattta gctgtattca ctaaatgtga agaagtctgt
      241 ctgtctataa caatataata tttattgatc cactctagtg ctttatctaa tgatgattct
      301 tgaaaagaga cttgctcttc tctagaaata gattggccga ttagaatgac tctattaaaa
      361 aagccttgat caacagcatc aaagtcagct tttgaacata ttccttgaga atacatgcca
      421 gcataagcga tacgaagagc gtaaagtaag ggcataaaag tactatgtga tggctgttca
      481 tataagctac ttaaataatc ctcaaggtct ttgaacatgt catagtactt tccttgtgct
      541 gtatggtttg aaggagtaat aatttctgtt aaatcatcgg gttctaggat agttttttta
      601 ctagctcctt gtattgagtt aatgacatca aaccatgctt gaatttcacc ttctgaaaga
      661 taagaacttc ccatctgaat gttgcttttt gcaaaagtta aacggctact atttaatgtg
      721 ttttgatttg cctctattaa cctttttata agcgtacatc ttatttttac agcctcatct
      781 attcctaact ctttaatcaa ctgggggctg acactattat ttaatattga tagtgggcta
      841 ttaggtaagt ccattgcgga ttctttattt agtgcatccc tatattcaat ttcatgaaat
      901 cgtgaaaaac ccactagttg ttttccatca ttgttccctt gtgatgctcc acaaagttcc
      961 tcaattaaaa aaagataaag ctcattgggt gctttaatac tgttacttaa ttctaaatag
     1021 gcattattta tgaatttttc taagtaaaca gggttcttta aggagttctg ctttttagaa
     1081 gaaaaaatac caaacatata ttgcactctt taaatttctt ttaattatta tactgtttct
     1141 atctattaga aatatctaaa ttttattaat aggaactgta tagatcttct acaattaaca
     1201 taatacacct tatacgtaat gcttgaattt ttcctataag catcaatatc ttagtctaag
     1261 ccgttctaga gttcagcgca ctggaacggc tttttcatga ctattcacgt tccacgccta
     1321 ttctttgacc aatgtttcac gactttgttc atattagtta aacaatcgtg gttaatcata
     1381 gggtctaaaa aaacggctcc aaaagcccct tttctccggt tcctgttcaa catgctccgg
     1441 cacgggaatc cgcttatttt cagtctgctc cggagtagtc aatccgtcat gtttcagctc
     1501 aggttctggc cgaggatccg ttgctatatt ctggccaggt ttctgatcgg taaacgtagt
     1561 catattgggt tttggggctt ctagcagtcg ttgtattgct tctatttgat tgtcttttgc
     1621 ttctagttgt tctcgttgga aaacttcccg ctcatgtgca agttctagct gtttttttag
     1681 tatgtctacc tgctgtttta gcatgtctac ttctgtctct ttttgtaccg cagtagactg
     1741 taacgtggtt acattactat ttttattaga tggttcgcca aatactcgta tggcttcaga
     1801 taaatctatt ttcccatcac tattttttga taaatctcct ttatttatac gcttataaat
     1861 actttgtcga ttcatcccat aaagttgtga taactcaata acagatagtg ctttcatcat
     1921 gtaacccaag taaaaatctg tatgcttgta gacaataatt aatcatgtaa cgttacatca
     1981 gcagtttaac gatagccaac tttttcaaga taaggatgta attcttgaaa cttagcagga
     2041 tcttgaagca tttcagcaat tcttattgca aactgttgat agctttcagt gccctgagag
     2101 tatttcccca tttcaggtag ttccgaaagc ttgttagcaa ataggtggcg ttgagcatct
     2161 gttattctgg aaaaaatatc taatgtattg ggatctttct tacgttctat tgttttattc
     2221 tgatttcttt tttgtttgaa gctaaaagag aatccagaaa tagagcgacc ttctttatgc
     2281 tgttcaactt taacgatgat atctgtatgt tcatttatct gtttgactgc tgattcaagt
     2341 acacgtcgtt taaatgtatc tagtcttggg taatcatctg aagttaagcc aagcttcgaa
     2401 cgaaagtcag aaacctcaaa tataggagtc ttaccgattt cacgccatgc tataagcaac
     2461 tcatagagtc gaatggcata ttttcctgtt aattgggtaa cttgttttaa ctgataactt
     2521 gtaaattgtt tttctaaacg tgtaattaag ggtactacat ccattgtaaa agtaacctct
     2581 aaggttgcag catcatcaat ataagaaata ttttgaaccc atcgagaaat aactttcttt
     2641 gattttcctg ttttttcatc aattgtttga tagctaaact gtctaccaaa aaggttagta
     2701 accgctgttt ttagggcttt gtaggcagca tgcttattaa cattaaatct gctcatatag
     2761 tcactggcat ggatccgtag cctagtgttt gcatcaatcc ctgttcttgt ttctcttgct
     2821 tgtaagatgg ctagtagaat taaccgttgt tctacggtat ccaaattata actcgcatta
     2881 attaaagcgt tatcttttac tattaattca ctggtaggca tttgctacac ctttattata
     2941 cgtggtgtta ttacaccatc attaatcaat ggtgtaaata ggtgactttt tggtgttgta
     3001 taggtgactt tttggtgttg tataggtgac tttttggtgt tgtataggtg actttttggt
     3061 gttgtatatc gattgtatat actataaata aaggctttca gtgtttttaa aagcatttaa
     3121 aatattaaaa ataattaaaa gccatggcaa agaaaaattg cccttggttt tcgctacgct
     3181 caaactctat tgaatctcgc tttcgctcga ttcgtggggc aattttttag atcattctgt
     3241 tgtgactttg agaaaaagca aaggcaagag caaaagcaac tcggaattcg cttcgctcat
     3301 aaagcttttt actcgctacg ctcgatctag gcccaatttt ctgcatgtga tttgcgctta
     3361 tttaggcttc acagcctaaa aagctgtttt tcaaagagtc gaggtgcgaa ttttgttgct
     3421 tcgctcgtag acactcgctc tattcatccc gattagaata aaaataacct ctatttggcg
     3481 ctcatttgcg ttttttgact tgattactca tttcaatagt ggattctatg tcttaagtaa
     3541 aaatgtggct gaaatcgcat gtaagagcgt tttaacttgg gttttatttt tgatctgtaa
     3601 gctagagttt gcgcttatag atttttctct tagaaacaca atagtcagaa tctcttttgg
     3661 ccaagcacta tctttactgt cagttaagtc aattaaatgg attgggagcc ggtacatacc
     3721 ggcaattttg ggttcacaca ccacgcatct aaagataaag gctctaaagc cttatgctat
     3781 aagggataca gcggtttttt atgtgtctgg aaagcgttta cgcttttatc atgattttta
     3841 ttatttttca tagatttagc tgtaaacact ttttgttgca gaaaaagtgc aatatctact
     3901 tacaaaacaa taggatagct tgtaatcggc tttgtttaca tgtttaggtt agtttctctt
     3961 tttatattaa atatttaacc tgtaataatt aaaacttaac acagtgttta cttatatttg
     4021 atagttactt tttagaaatt aggtgcttag attgaaaaca aatgatagga gtttttcatg
     4081 aacagtactc gaaccgttgc cgtcagatta gacaatcaag tcgcacaaga atatgagcgt
     4141 ttggctcagt tgaagggcga taaacttgga acttattt
//
